RLG00000008807

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002695
Physical Location & Seq
Reverse (-)
39723521 .. 39725933
2413 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008807

Sequence Viewer

Length: 582 bp
ATGTTGATCTGGTGCCAGATTCCAAATTCTAACAAGACTGATAAGGCTTCAATGCTGGATGAAGCAATTGAGTATTTGAAGCAGCTCCAGCTTCAAGTACAGGTCGATACAAGAGGCGAGCCCGGGCCGCGCTCCGGTGTAAGGCGGCCGGAAAACGCCTTGCCGGTTCAAAATGAGACTTGCTGCTGGGAAAAGCGTCCGCCCAGGCGTCGGGGCGGCGTTTTTTTGCAGAAGCAGAGCATAAAAGCGACAGAGCGAGAGGAAGAAGATGAAAAAGAGAAATGGAAGAGACAAAATATGAGAAAACTTACTGCATTACTTAGGGATGAAGATGATGAGATAGAAGACCTTACTTTGGCTCCCCACACAGATGCAGCATTGAACATTGATGATTGTGATGATATTCATCCGGGAGCTGACTATATTATTTACATCATAGATGTATCTGGAGGTGATAAAATTCCACGAAAAGGTGGCCCTGGAATCACTCAAGCTGACCTTCTGGTGATAAACAAGACTGACCTTGCACAAGCAGTTGGAGTTGATTTCGCAGTGATGGAGTGTGATGCACTTCGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

194

Amino Acids

21.83

Weight (kDa)

4.86

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 139 - 189 4.6e-08 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 12
AccII CGCG 1 cut(s) 130
AciI CCGC 4 cut(s) 128, 145, 200, 216
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 2 cut(s) 25, 459
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 5 cut(s) 134, 141, 210, 355, 470
AgsI TTSAA 5 cut(s) 51, 79, 95, 170, 382
AjnI CCWGG 2 cut(s) 203, 478
AluBI AGCT 4 cut(s) 85, 91, 416, 494
AluI AGCT 4 cut(s) 85, 91, 416, 494
Alw26I GTCTC 2 cut(s) 170, 283
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 3 cut(s) 125, 146, 475
ApeKI GCWGC 3 cut(s) 82, 183, 374
ApoI RAATTY 2 cut(s) 25, 459
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 125, 476
AsuC2I CCSGG 3 cut(s) 123, 124, 411
AsuHPI GGTGA 2 cut(s) 464, 517
AsuII TTCGAA 1 cut(s) 574
AvaI CYCGRG 1 cut(s) 122
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 94, 170, 386
BccI CCATC 1 cut(s) 550
BciT130I CCWGG 2 cut(s) 205, 480
BcnI CCSGG 3 cut(s) 123, 124, 411
BcoDI GTCTC 2 cut(s) 170, 283
BisI GCNGC 6 cut(s) 83, 128, 146, 184, 217, 375
BlsI GCNGC 6 cut(s) 84, 129, 147, 185, 218, 376
Bme1390I CCNGG 5 cut(s) 123, 124, 205, 411, 480
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 125, 476
BmiI GGNNCC 2 cut(s) 14, 360
BmrFI CCNGG 5 cut(s) 123, 124, 205, 411, 480
BmsI GCATC 2 cut(s) 361, 556
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 2 cut(s) 71, 468
Bpu14I TTCGAA 1 cut(s) 574
BpuEI CTTGAG 1 cut(s) 474
BpuMI CCSGG 3 cut(s) 123, 124, 411
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 3 cut(s) 122, 203, 478
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 141, 210, 355, 470
Bse118I RCCGGY 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 2 cut(s) 205, 480
BseDI CCNNGG 3 cut(s) 122, 203, 478
BseGI GGATG 3 cut(s) 64, 331, 406
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 5 cut(s) 134, 141, 210, 355, 470
BseX3I CGGCCG 1 cut(s) 146
BseXI GCAGC 3 cut(s) 94, 170, 386
BseYI CCCAGC 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 130
Bsh1285I CGRYCG 1 cut(s) 149
BshFI GGCC 3 cut(s) 127, 148, 477
BshNI GGYRCC 1 cut(s) 12
BsiEI CGRYCG 1 cut(s) 149
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 5 cut(s) 123, 135, 149, 164, 410
BslI CCNNNNNNNGG 5 cut(s) 134, 141, 210, 355, 470
BsmAI GTCTC 2 cut(s) 170, 283
BsnI GGCC 3 cut(s) 127, 148, 477
BsoBI CYCGRG 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 574
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 4 cut(s) 128, 145, 200, 216
BspANI GGCC 3 cut(s) 127, 148, 477
BspFNI CGCG 1 cut(s) 130
BspLI GGNNCC 2 cut(s) 14, 360
BspT104I TTCGAA 1 cut(s) 574
BspT107I GGYRCC 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 3 cut(s) 122, 203, 478
BssMI GATC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 205, 480
Bst6I CTCTTC 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 208
BstBI TTCGAA 1 cut(s) 574
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 320
BstF5I GGATG 3 cut(s) 64, 331, 406
BstFNI CGCG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 2 cut(s) 170, 283
BstMBI GATC 1 cut(s) 6
BstMCI CGRYCG 1 cut(s) 149
BstMWI GCNNNNNNNGC 2 cut(s) 88, 127
BstNI CCWGG 2 cut(s) 205, 480
BstSCI CCNGG 5 cut(s) 121, 122, 203, 409, 478
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 3 cut(s) 94, 170, 386
BstV2I GAAGAC 1 cut(s) 351
BstZI CGGCCG 1 cut(s) 146
BsuRI GGCC 3 cut(s) 127, 148, 477
BtsCI GGATG 3 cut(s) 64, 331, 406
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 1 cut(s) 132
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 125, 476
Cfr9I CCCGGG 1 cut(s) 122
CseI GACGC 2 cut(s) 185, 197
Csp6I GTAC 1 cut(s) 98
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 1 cut(s) 320
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 146
EagI CGGCCG 1 cut(s) 146
Eam1104I CTCTTC 1 cut(s) 281
EarI CTCTTC 1 cut(s) 281
EciI GGCGGA 1 cut(s) 189
EclXI CGGCCG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 123
Eco52I CGGCCG 1 cut(s) 146
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 203, 478
EcoT38I GRGCYC 1 cut(s) 123
FaiI YATR 4 cut(s) 242, 299, 423, 437
FalI AAGNNNNNCTT 2 cut(s) 483, 515
Fnu4HI GCNGC 6 cut(s) 83, 128, 146, 184, 217, 375
FokI GGATG 3 cut(s) 71, 338, 393
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 6 cut(s) 83, 128, 146, 184, 217, 375
GlaI GCGC 1 cut(s) 131
GluI GCNGC 6 cut(s) 83, 128, 146, 184, 217, 375
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 2 cut(s) 71, 468
HaeIII GGCC 3 cut(s) 127, 148, 477
HapII CCGG 5 cut(s) 123, 135, 149, 164, 410
HgaI GACGC 2 cut(s) 185, 197
HhaI GCGC 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 208
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HinfI GANTC 2 cut(s) 19, 483
HpaII CCGG 5 cut(s) 123, 135, 149, 164, 410
HphI GGTGA 2 cut(s) 464, 517
Hpy188III TCNNGA 1 cut(s) 447
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 1 cut(s) 509
HpyCH4V TGCA 5 cut(s) 229, 314, 374, 527, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 127
HpyF3I CTNAG 1 cut(s) 320
Hsp92I GRCGYC 1 cut(s) 208
HspAI GCGC 1 cut(s) 130
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 4 cut(s) 90, 137, 364, 413
Lsp1109I GCAGC 3 cut(s) 94, 170, 386
LweI GCATC 2 cut(s) 361, 556
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 5 cut(s) 275, 278, 298, 341, 356
MfeI CAATTG 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 3 cut(s) 25, 66, 459
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 3 cut(s) 107, 253, 443
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 5 cut(s) 123, 135, 149, 164, 410
MspR9I CCNGG 5 cut(s) 123, 124, 205, 411, 480
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 2 cut(s) 205, 480
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 88, 127
NciI CCSGG 3 cut(s) 123, 124, 411
NdeII GATC 1 cut(s) 6
NlaIV GGNNCC 2 cut(s) 14, 360
NspV TTCGAA 1 cut(s) 574
PfeI GAWTC 2 cut(s) 19, 483
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 6 cut(s) 84, 129, 147, 185, 218, 376
Psp6I CCWGG 2 cut(s) 203, 478
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 2 cut(s) 203, 478
PspN4I GGNNCC 2 cut(s) 14, 360
PspPI GGNCC 2 cut(s) 125, 476
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 369
SatI GCNGC 6 cut(s) 83, 128, 146, 184, 217, 375
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 2 cut(s) 125, 476
ScrFI CCNGG 5 cut(s) 123, 124, 205, 411, 480
SduI GDGCHC 1 cut(s) 123
SfaNI GCATC 2 cut(s) 361, 556
SfuI TTCGAA 1 cut(s) 574
SmaI CCCGGG 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
SrfI GCCCGGGC 1 cut(s) 124
Sse9I AATT 3 cut(s) 25, 66, 459
SsiI CCGC 4 cut(s) 128, 145, 200, 216
StyD4I CCNGG 5 cut(s) 121, 122, 203, 409, 478
TaqI TCGA 2 cut(s) 105, 574
TasI AATT 3 cut(s) 25, 66, 459
TatI WGTACW 1 cut(s) 97
TauI GCSGC 3 cut(s) 130, 148, 219
TfiI GAWTC 2 cut(s) 19, 483
TscAI CASTG 1 cut(s) 558
TseI GCWGC 3 cut(s) 82, 183, 374
TspDTI ATGAA 4 cut(s) 75, 285, 342, 395
TspMI CCCGGG 1 cut(s) 122
TspRI CASTG 1 cut(s) 558
XapI RAATTY 2 cut(s) 25, 459
XmaI CCCGGG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.