pycom07g08170

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002695
Physical Location & Seq
Reverse (-)
7554238 .. 7558180
3943 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g08170.3

Sequence Viewer

Length: 1779 bp
ATGCTCTGTTTTATTTGTGATTTGTGTAACGTGCCTGAAGTGTGTTTCAGTACCGTGGAAGAAAATGTTGATAGTAACCAGCCTGGCGATTCTGGTGTGTCTAGGTTGAATGGTGAAAGTTTTTCAGAGGGTTCCAAGTCTGAAAAGAATATAGAGGGTGGTGCTTTATGGGATATTTTCCGGCGTGAAGATGTTCCTAAGCTGAACGATTATCTCCAGAAGCACTGCAAGGAATTCAGGCATACTTTCTGTTGTCCAGTGCAGCAGCCATGCATTAAAGTTGCACTTGAATTTGTCTCACCTGAAAATGTCGGTGAGTGTATTCGGTTGACCGATGAGTTCCGCACACTTCCAAAAAATCACAGGGCTAAAGAGGACAAATTGGAGGTAAAGAAAATGATTTTGCATTCTGTCCATCAGGTTGTGGAGAAGTTGGATACAAATATGAGACATGGATTGAGAAATGAAGCTGTGGAAAGACCAAAAAAAGGAGAGAAGAGGAGGAAGTCGAAGCTGTTGACAAGAAGGCCGCAGAAGGGAGGGGTGAAGAATAGGAGGTCCAAAGTTGTTGAGGAGCAGCGTTCACTGTGCCCTCCTGAACAATGCAGAAATGTATGTGCATGGAGAGAGGAAGAATCTTCCGGACAACAAGAAGGAATGGAAAAAGAGGAGGTTCAAGTTGTTGAGGAGCGTTCACTGCCCCCTCTTGAACAATGCAGAAATGTGCATGCATGGAGAGAGAAAGAATCTTCTGGACAACAAGAAGGAATGGAAATTGAGGTTCAAGTTGTTTTGGAGCAGCAGTCTTCACAGCCACCTGTTGAACTATGTAGAATTGAACATGCATGCAGAAGGAGTGGTGATGAGGAAGAGGCCCATGGATCCGAGGAGCAGCGATTCTTACTTCCTGCACTGCCCCCTCTTGAACGATGCATAAATGTGCACGAGGAAGAATCTTCCGGACAACAAAAAGGAATGGAAATCAAGGAGGTTCAAATTGTTGAGGAGCAGCGTGCACTGCCCCCTCTTGAACAATGCAGAAATGCGCATGCATGGAGAGAGGAAGAATCTTCCGGACAACAAGAAGGAATGGAAATTGAGGAGGTTCAAGCTGTTTTGGAGCAGCAGACTTCACGGCCACCTGTTGAAATAGGTAGAATTGAAAAGGCATGCAGAAGGGAAGAAGATGGAGGACAGACAGAAGGAAGTGGTGATGAGGAAGAGGCCCAAGGATCTGAGGAGCAGCGATTCTTACTTTCTGCACATTCCACTGTTCAAGAGCGAAGCATTGGACATGCATGGAGAGAGCTAGAAGGTGGAGGACAACAACAAAAAGGAAGGGCAAAAGGGGAAGAGTCAGTTGCGAAGAAGCCTCCACAATCATCACAGTCTGAAGAAGAGGATGATGTGATTATTGTGGAGAAGCCTCCACAATCATCACAGTCTGAAGAAGACGACGATGACGTGGTTATTGTAGAGAAGCCTTCGCAATCATCACATTCACGAGTCTCTAGACACACCAATGTTGGTCTTGTTACATTGGTGAAGTATCTCGATAACCTCACTGAGCGCATTACACATTTTGAACAGACAACAGAACAGAGGGATAGAATACAGAATCCTGAATTCCAGCATATCCGTTCAGAGCTTTTGACTTTAGCTTCCCGTCTTGGTTACACAACTCATCCGAGAGAGCCTGACATCCCACCATCATTGTCTCAGTCACCAAAAGAAGGCTGTCCGCCTCTCCTTGTTTACCGAAGAAGATCCACCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04714 map04714
Pfam Domains
Protein Families
JmjC zf-4CXXC_R1

Protein Analysis

593

Amino Acids

67.51

Weight (kDa)

5.22

Isoelectric Point (pI)

81.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1047
AccIII TCCGGA 3 cut(s) 641, 959, 1073
AciI CCGC 3 cut(s) 343, 530, 1742
AclWI GGATC 4 cut(s) 876, 889, 1240, 1761
AcoI YGGCCR 1 cut(s) 1136
AcsI RAATTY 3 cut(s) 233, 290, 1625
AcuI CTGAAG 3 cut(s) 57, 1413, 1467
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 3 cut(s) 488, 536, 1733
AjiI CACGTC 1 cut(s) 1465
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 7 cut(s) 202, 470, 514, 1112, 1309, 1648, 1661
AluI AGCT 7 cut(s) 202, 470, 514, 1112, 1309, 1648, 1661
Alw21I GWGCWC 2 cut(s) 945, 1018
Alw26I GTCTC 4 cut(s) 301, 442, 1513, 1722
Alw44I GTGCAC 2 cut(s) 941, 1014
AlwI GGATC 4 cut(s) 876, 889, 1240, 1761
Aor13HI TCCGGA 3 cut(s) 641, 959, 1073
AoxI GGCC 4 cut(s) 527, 873, 1136, 1224
ApaLI GTGCAC 2 cut(s) 941, 1014
ApeKI GCWGC 8 cut(s) 262, 265, 577, 799, 892, 1009, 1123, 1243
ApoI RAATTY 3 cut(s) 233, 290, 1625
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 2 cut(s) 1048, 1572
AspS9I GGNCC 3 cut(s) 558, 874, 1225
AsuHPI GGTGA 8 cut(s) 125, 291, 326, 556, 872, 1223, 1555, 1716
AvaII GGWCC 1 cut(s) 558
BaeGI GKGCMC 3 cut(s) 593, 945, 1018
BamHI GGATCC 1 cut(s) 881
BauI CACGAG 2 cut(s) 944, 1503
BbsI GAAGAC 2 cut(s) 798, 1458
Bbv12I GWGCWC 2 cut(s) 945, 1018
BbvI GCAGC 8 cut(s) 274, 277, 589, 811, 904, 1021, 1135, 1255
BccI CCATC 3 cut(s) 423, 1181, 1717
BceAI ACGGC 1 cut(s) 1151
BciT130I CCWGG 1 cut(s) 84
BciVI GTATCC 1 cut(s) 430
BcoDI GTCTC 4 cut(s) 301, 442, 1513, 1722
BfaI CTAG 3 cut(s) 102, 1310, 1512
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 9 cut(s) 263, 266, 530, 578, 800, 893, 1010, 1124, 1244
BlsI GCNGC 9 cut(s) 264, 267, 531, 579, 801, 894, 1011, 1125, 1245
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 558
BmgBI CACGTC 1 cut(s) 1465
BmgT120I GGNCC 3 cut(s) 558, 874, 1225
BmiI GGNNCC 2 cut(s) 133, 883
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 920
BpiI GAAGAC 2 cut(s) 798, 1458
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 198
BsaJI CCNNGG 4 cut(s) 54, 877, 885, 1228
BsaWI WCCGGW 3 cut(s) 641, 959, 1073
BsaXI ACNNNNNCTCC 2 cut(s) 788, 818
Bsc4I CCNNNNNNNGG 3 cut(s) 488, 536, 1733
Bse118I RCCGGY 1 cut(s) 1772
Bse1I ACTGG 1 cut(s) 257
BseAI TCCGGA 3 cut(s) 641, 959, 1073
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 4 cut(s) 54, 877, 885, 1228
BseGI GGATG 3 cut(s) 1408, 1684, 1701
BseLI CCNNNNNNNGG 3 cut(s) 488, 536, 1733
BseMII CTCAG 3 cut(s) 1227, 1557, 1733
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 8 cut(s) 514, 587, 683, 701, 902, 1019, 1115, 1253
BseSI GKGCMC 3 cut(s) 593, 945, 1018
BseXI GCAGC 8 cut(s) 274, 277, 589, 811, 904, 1021, 1135, 1255
BsgI GTGCAG 3 cut(s) 281, 894, 1245
BshFI GGCC 4 cut(s) 529, 875, 1138, 1226
BsiHKAI GWGCWC 2 cut(s) 945, 1018
BsiSI CCGG 5 cut(s) 181, 642, 960, 1074, 1773
BslI CCNNNNNNNGG 3 cut(s) 488, 536, 1733
BsmAI GTCTC 4 cut(s) 301, 442, 1513, 1722
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 4 cut(s) 529, 875, 1138, 1226
Bsp1286I GDGCHC 3 cut(s) 593, 945, 1018
Bsp13I TCCGGA 3 cut(s) 641, 959, 1073
Bsp143I GATC 3 cut(s) 881, 1232, 1766
Bsp19I CCATGG 1 cut(s) 877
BspACI CCGC 3 cut(s) 343, 530, 1742
BspANI GGCC 4 cut(s) 529, 875, 1138, 1226
BspCNI CTCAG 3 cut(s) 1228, 1558, 1732
BspEI TCCGGA 3 cut(s) 641, 959, 1073
BspLI GGNNCC 2 cut(s) 133, 883
BspPI GGATC 4 cut(s) 876, 889, 1240, 1761
BsrFI RCCGGY 1 cut(s) 1772
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 1772
BssECI CCNNGG 4 cut(s) 54, 877, 885, 1228
BssMI GATC 3 cut(s) 881, 1232, 1766
BssSI CACGAG 2 cut(s) 944, 1503
BssT1I CCWWGG 2 cut(s) 877, 1228
Bst2BI CACGAG 2 cut(s) 944, 1503
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 5 cut(s) 55, 588, 1273, 1389, 1443
Bst6I CTCTTC 5 cut(s) 491, 864, 1215, 1347, 1392
BstC8I GCNNGC 5 cut(s) 729, 847, 1014, 1050, 1171
BstDEI CTNAG 4 cut(s) 198, 1236, 1566, 1719
BstDSI CCRYGG 2 cut(s) 54, 877
BstF5I GGATG 3 cut(s) 1408, 1684, 1701
BstHHI GCGC 2 cut(s) 1048, 1572
BstKTI GATC 3 cut(s) 884, 1235, 1769
BstMAI GTCTC 4 cut(s) 301, 442, 1513, 1722
BstMBI GATC 3 cut(s) 881, 1232, 1766
BstMWI GCNNNNNNNGC 2 cut(s) 697, 1018
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 6 cut(s) 731, 845, 849, 1052, 1173, 1298
BstSCI CCNGG 1 cut(s) 82
BstSLI GKGCMC 3 cut(s) 593, 945, 1018
BstV1I GCAGC 8 cut(s) 274, 277, 589, 811, 904, 1021, 1135, 1255
BstV2I GAAGAC 2 cut(s) 798, 1458
BstX2I RGATCY 3 cut(s) 881, 1232, 1766
BstYI RGATCY 3 cut(s) 881, 1232, 1766
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 4 cut(s) 529, 875, 1138, 1226
BtgI CCRYGG 2 cut(s) 54, 877
BtrI CACGTC 1 cut(s) 1465
BtsCI GGATG 3 cut(s) 1408, 1684, 1701
BtsI GCAGTG 4 cut(s) 223, 695, 911, 1016
BtsIMutI CAGTG 8 cut(s) 223, 264, 584, 695, 911, 1016, 1269, 1563
Cac8I GCNNGC 5 cut(s) 729, 847, 1014, 1050, 1171
CfoI GCGC 2 cut(s) 1048, 1572
Cfr10I RCCGGY 1 cut(s) 1772
Cfr13I GGNCC 3 cut(s) 558, 874, 1225
Csp6I GTAC 1 cut(s) 51
CspCI CAANNNNNGTGG 2 cut(s) 1695, 1730
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 4 cut(s) 198, 1236, 1566, 1719
DpnI GATC 3 cut(s) 883, 1234, 1768
DpnII GATC 3 cut(s) 881, 1232, 1766
EaeI YGGCCR 1 cut(s) 1136
Eam1104I CTCTTC 5 cut(s) 491, 864, 1215, 1347, 1392
EarI CTCTTC 5 cut(s) 491, 864, 1215, 1347, 1392
EciI GGCGGA 1 cut(s) 1731
Eco130I CCWWGG 2 cut(s) 877, 1228
Eco47I GGWCC 1 cut(s) 558
Eco57I CTGAAG 3 cut(s) 57, 1413, 1467
EcoRI GAATTC 2 cut(s) 233, 1625
EcoRII CCWGG 1 cut(s) 82
EcoT14I CCWWGG 2 cut(s) 877, 1228
EcoT22I ATGCAT 6 cut(s) 275, 733, 847, 935, 1054, 1300
ErhI CCWWGG 2 cut(s) 877, 1228
FalI AAGNNNNNCTT 2 cut(s) 270, 302
Fnu4HI GCNGC 9 cut(s) 263, 266, 530, 578, 800, 893, 1010, 1124, 1244
FokI GGATG 3 cut(s) 1415, 1671, 1688
Fsp4HI GCNGC 9 cut(s) 263, 266, 530, 578, 800, 893, 1010, 1124, 1244
FspAI RTGCGCAY 1 cut(s) 1047
FspBI CTAG 3 cut(s) 102, 1310, 1512
FspI TGCGCA 1 cut(s) 1047
GlaI GCGC 2 cut(s) 1047, 1571
GluI GCNGC 9 cut(s) 263, 266, 530, 578, 800, 893, 1010, 1124, 1244
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 4 cut(s) 529, 875, 1138, 1226
HapII CCGG 5 cut(s) 181, 642, 960, 1074, 1773
HhaI GCGC 2 cut(s) 1048, 1572
Hin6I GCGC 2 cut(s) 1046, 1570
HinP1I GCGC 2 cut(s) 1046, 1570
HincII GTYRAC 2 cut(s) 330, 519
HindII GTYRAC 2 cut(s) 330, 519
HpaII CCGG 5 cut(s) 181, 642, 960, 1074, 1773
HphI GGTGA 8 cut(s) 125, 291, 326, 556, 872, 1223, 1555, 1716
Hpy166II GTNNAC 7 cut(s) 330, 519, 584, 695, 943, 1016, 1756
Hpy188I TCNGA 8 cut(s) 127, 142, 886, 1237, 1393, 1447, 1645, 1689
Hpy8I GTNNAC 7 cut(s) 330, 519, 584, 695, 943, 1016, 1756
Hpy99I CGWCG 1 cut(s) 1460
HpyCH4III ACNGT 5 cut(s) 55, 588, 1273, 1389, 1443
HpyCH4IV ACGT 2 cut(s) 30, 1464
HpyF10VI GCNNNNNNNGC 2 cut(s) 697, 1018
HpyF3I CTNAG 4 cut(s) 198, 1236, 1566, 1719
HpySE526I ACGT 2 cut(s) 30, 1464
HspAI GCGC 2 cut(s) 1046, 1570
Kpn2I TCCGGA 3 cut(s) 641, 959, 1073
Kzo9I GATC 3 cut(s) 881, 1232, 1766
LmnI GCTCC 7 cut(s) 574, 688, 796, 889, 1006, 1120, 1240
Lsp1109I GCAGC 8 cut(s) 274, 277, 589, 811, 904, 1021, 1135, 1255
LweI GCATC 1 cut(s) 920
MaeI CTAG 3 cut(s) 102, 1310, 1512
MaeII ACGT 2 cut(s) 30, 1464
MaeIII GTNAC 5 cut(s) 26, 74, 1534, 1673, 1722
MalI GATC 3 cut(s) 883, 1234, 1768
MboI GATC 3 cut(s) 881, 1232, 1766
MflI RGATCY 3 cut(s) 881, 1232, 1766
MhlI GDGCHC 3 cut(s) 593, 945, 1018
MluCI AATT 9 cut(s) 233, 290, 380, 774, 834, 996, 1095, 1158, 1625
MlyI GAGTC 2 cut(s) 1364, 1515
MmeI TCCRAC 1 cut(s) 414
Mph1103I ATGCAT 6 cut(s) 275, 733, 847, 935, 1054, 1300
MroI TCCGGA 3 cut(s) 641, 959, 1073
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 2 cut(s) 938, 1521
MspI CCGG 5 cut(s) 181, 642, 960, 1074, 1773
MspR9I CCNGG 1 cut(s) 84
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 697, 1018
NcoI CCATGG 1 cut(s) 877
NdeII GATC 3 cut(s) 881, 1232, 1766
NlaIV GGNNCC 2 cut(s) 133, 883
NmuCI GTSAC 1 cut(s) 1722
NsbI TGCGCA 1 cut(s) 1047
NsiI ATGCAT 6 cut(s) 275, 733, 847, 935, 1054, 1300
NspI RCATGY 6 cut(s) 731, 845, 849, 1052, 1173, 1298
PaeI GCATGC 4 cut(s) 731, 849, 1052, 1173
PcsI WCGNNNNNNNCGW 1 cut(s) 1461
PctI GAATGC 1 cut(s) 406
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 8 cut(s) 89, 635, 746, 897, 953, 1067, 1248, 1618
PkrI GCNGC 9 cut(s) 264, 267, 531, 579, 801, 894, 1011, 1125, 1245
PleI GAGTC 2 cut(s) 1363, 1514
PpsI GAGTC 2 cut(s) 1363, 1514
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 2 cut(s) 133, 883
PspPI GGNCC 3 cut(s) 558, 874, 1225
PsuI RGATCY 3 cut(s) 881, 1232, 1766
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 2 cut(s) 938, 1521
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 9 cut(s) 263, 266, 530, 578, 800, 893, 1010, 1124, 1244
Sau3AI GATC 3 cut(s) 881, 1232, 1766
Sau96I GGNCC 3 cut(s) 558, 874, 1225
SchI GAGTC 2 cut(s) 1364, 1515
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 3 cut(s) 593, 945, 1018
SfaNI GCATC 1 cut(s) 920
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 938, 1521
SphI GCATGC 4 cut(s) 731, 849, 1052, 1173
Sse9I AATT 9 cut(s) 233, 290, 380, 774, 834, 996, 1095, 1158, 1625
SsiI CCGC 3 cut(s) 343, 530, 1742
SspMI CTAG 3 cut(s) 102, 1310, 1512
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 2 cut(s) 877, 1228
TaaI ACNGT 5 cut(s) 55, 588, 1273, 1389, 1443
TaiI ACGT 2 cut(s) 33, 1467
TaqI TCGA 2 cut(s) 509, 1554
TaqII GACCGA 1 cut(s) 347
TasI AATT 9 cut(s) 233, 290, 380, 774, 834, 996, 1095, 1158, 1625
TauI GCSGC 1 cut(s) 532
TfiI GAWTC 8 cut(s) 89, 635, 746, 897, 953, 1067, 1248, 1618
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 8 cut(s) 230, 264, 591, 702, 918, 1023, 1276, 1570
TseFI GTSAC 1 cut(s) 1722
TseI GCWGC 8 cut(s) 262, 265, 577, 799, 892, 1009, 1123, 1243
Tsp45I GTSAC 1 cut(s) 1722
TspDTI ATGAA 1 cut(s) 480
TspGWI ACGGA 1 cut(s) 1628
TspRI CASTG 8 cut(s) 230, 264, 591, 702, 918, 1023, 1276, 1570
VneI GTGCAC 2 cut(s) 941, 1014
VpaK11BI GGWCC 1 cut(s) 558
XapI RAATTY 3 cut(s) 233, 290, 1625
XbaI TCTAGA 1 cut(s) 1511
XceI RCATGY 6 cut(s) 731, 845, 849, 1052, 1173, 1298
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 3 cut(s) 102, 1310, 1512
Zsp2I ATGCAT 6 cut(s) 275, 733, 847, 935, 1054, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.