Rorug07G0172100
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003951
Physical Location & Seq
Forward (+)
14053791 .. 14056401
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0172100.1

Sequence Viewer

Length: 858 bp
ATGGTGAAAGTCAAAAGGGAACAAATAGAGGAGGCTGTTATGCATTCCGACAGGAAATTTGGGGTTGAGTCGTGGCCATTATCTGAAGGCAATTGTTACTCGCCTGTTTCAGAGCCAAGCCCTCTTAGTTGGAGAACCAGTGGTCCAACCAGGCGATCATCAAGATGCTGGACAGAGAAAGAGGATAAACTTCTGGTAGAGCTGGTCAGAAAGTTTAATGGGAGTAATTGGAAGCAAATAGGTATCCCTGGCCGTTCAGATGTTCAGTGCCTGCATCGCTGGCAAAAAGTTTTGAATCCTGAAATCATCAAGGGATACTGGACAAAGGAGGAAGATGATTGTATAATCAAACTGGTTGAAAAGTATGGTGCTAGAAGATGGTCTGTCATTGCAAAGTTTTTACCAGGTCGAATAGGCAAGCAATGCAGAGAAAGGTGGTACAATCATTTAAGCCTGGCTATAAACAAAGATGCATGGACAGAAGAAGTGGAAAGAATTCTTTCTTTCTACCACCAACAGTATGGTAACAAGTGGGCAGAAATAGCAAGGTTTCTACCTGGAAGGACTGACAATGCAATTAAAAATTACTGGAATGGCGCATTCAAGAGGAAAGAAGATTCAAACTCTCCTAATGGTAATGAGTGGGACATGCATCCTGATATCTGTATTGATGAACTAAGATCAGATTTTGTAGGGGTTAAAACAGTCAGACAGAAGTATGTGAAATCACAGAAATGCAGGTACTTAGCCGCCGGCGAAATTGGTTGGTGGCCTCCTTGGCCTGACATCGACTCGGCCACCAAGATGATTTTCCTCGAGCTCCTGCTTCCAGATCTCACTCTACACTATTTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

285

Amino Acids

33.54

Weight (kDa)

8.94

Isoelectric Point (pI)

54.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 56 - 98 1.2e-12 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 57 - 109 5.3e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 104 - 150 8.3e-20 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 107 - 165 1.9e-16 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 156 - 198 3.4e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 159 - 203 2.8e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 729
AciI CCGC 1 cut(s) 750
AcoI YGGCCR 3 cut(s) 74, 250, 795
AcsI RAATTY 2 cut(s) 56, 495
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 2 cut(s) 440, 743
AgsI TTSAA 4 cut(s) 295, 359, 604, 621
AjnI CCWGG 5 cut(s) 149, 247, 403, 453, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 794, 826
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
AluBI AGCT 2 cut(s) 202, 820
AluI AGCT 2 cut(s) 202, 820
Alw21I GWGCWC 1 cut(s) 822
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 5 cut(s) 74, 250, 770, 779, 795
ApoI RAATTY 2 cut(s) 56, 495
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
Asp700I GAANNNNTTC 2 cut(s) 495, 499
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 1 cut(s) 143
BalI TGGCCA 1 cut(s) 76
BanII GRGCYC 1 cut(s) 822
Bbv12I GWGCWC 1 cut(s) 822
BccI CCATC 1 cut(s) 372
BceAI ACGGC 1 cut(s) 237
BciT130I CCWGG 5 cut(s) 151, 249, 405, 455, 558
BciVI GTATCC 2 cut(s) 254, 308
BfaI CTAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 729
BfuI GTATCC 2 cut(s) 254, 308
BglI GCCNNNNNGGC 1 cut(s) 778
BglII AGATCT 1 cut(s) 832
BisI GCNGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 751
Bme1390I CCNGG 5 cut(s) 151, 249, 405, 455, 558
Bme18I GGWCC 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 1 cut(s) 143
BmrFI CCNGG 5 cut(s) 151, 249, 405, 455, 558
BmsI GCATC 4 cut(s) 155, 283, 460, 661
BsaJI CCNNGG 2 cut(s) 247, 776
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 4 cut(s) 138, 323, 357, 593
Bse3DI GCAATG 2 cut(s) 387, 428
BseBI CCWGG 5 cut(s) 151, 249, 405, 455, 558
BseDI CCNNGG 2 cut(s) 247, 776
BseGI GGATG 1 cut(s) 652
BseMI GCAATG 2 cut(s) 387, 428
BseNI ACTGG 4 cut(s) 138, 323, 357, 593
BseRI GAGGAG 1 cut(s) 44
BshFI GGCC 5 cut(s) 76, 252, 772, 781, 797
BsiHKAI GWGCWC 1 cut(s) 822
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 1 cut(s) 753
BslFI GGGAC 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 659
BsmI GAATGC 2 cut(s) 43, 599
BsnI GGCC 5 cut(s) 76, 252, 772, 781, 797
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 3 cut(s) 155, 680, 832
BspACI CCGC 1 cut(s) 750
BspANI GGCC 5 cut(s) 76, 252, 772, 781, 797
BspHI TCATGA 1 cut(s) 854
BspMI ACCTGC 1 cut(s) 729
BsrDI GCAATG 2 cut(s) 387, 428
BsrFI RCCGGY 1 cut(s) 752
BsrI ACTGG 4 cut(s) 138, 323, 357, 593
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 2 cut(s) 247, 776
BssMI GATC 3 cut(s) 155, 680, 832
BssT1I CCWWGG 1 cut(s) 776
Bst2UI CCWGG 5 cut(s) 151, 249, 405, 455, 558
Bst4CI ACNGT 2 cut(s) 519, 706
BstAPI GCANNNNNTGC 1 cut(s) 423
BstC8I GCNNGC 4 cut(s) 272, 281, 419, 754
BstDEI CTNAG 3 cut(s) 125, 677, 745
BstF5I GGATG 1 cut(s) 652
BstHHI GCGC 1 cut(s) 599
BstKTI GATC 3 cut(s) 158, 683, 835
BstMBI GATC 3 cut(s) 155, 680, 832
BstMWI GCNNNNNNNGC 5 cut(s) 276, 280, 423, 542, 778
BstNI CCWGG 5 cut(s) 151, 249, 405, 455, 558
BstNSI RCATGY 1 cut(s) 652
BstSCI CCNGG 5 cut(s) 149, 247, 403, 453, 556
BstX2I RGATCY 1 cut(s) 832
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 1 cut(s) 832
BsuI GTATCC 2 cut(s) 254, 308
BsuRI GGCC 5 cut(s) 76, 252, 772, 781, 797
BtgZI GCGATG 1 cut(s) 260
BtsCI GGATG 1 cut(s) 652
BtsIMutI CAGTG 2 cut(s) 145, 272
BveI ACCTGC 1 cut(s) 729
Cac8I GCNNGC 4 cut(s) 272, 281, 419, 754
CaiI CAGNNNCTG 1 cut(s) 271
CciI TCATGA 1 cut(s) 854
CfoI GCGC 1 cut(s) 599
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 1 cut(s) 143
CsiI ACCWGGT 1 cut(s) 403
Csp6I GTAC 2 cut(s) 439, 742
CviAII CATG 3 cut(s) 474, 649, 855
CviQI GTAC 2 cut(s) 439, 742
DdeI CTNAG 3 cut(s) 125, 677, 745
DpnI GATC 3 cut(s) 157, 682, 834
DpnII GATC 3 cut(s) 155, 680, 832
EaeI YGGCCR 3 cut(s) 74, 250, 795
Ecl136II GAGCTC 1 cut(s) 820
Eco130I CCWWGG 1 cut(s) 776
Eco24I GRGCYC 1 cut(s) 822
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 820
Eco57I CTGAAG 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 820
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 5 cut(s) 149, 247, 403, 453, 556
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 776
EcoT22I ATGCAT 3 cut(s) 45, 475, 654
EcoT38I GRGCYC 1 cut(s) 822
ErhI CCWWGG 1 cut(s) 776
FaeI CATG 3 cut(s) 477, 652, 858
FaiI YATR 9 cut(s) 41, 344, 366, 461, 475, 522, 650, 720, 856
FaqI GGGAC 1 cut(s) 659
FatI CATG 3 cut(s) 473, 648, 854
Fnu4HI GCNGC 1 cut(s) 750
FokI GGATG 1 cut(s) 639
FriOI GRGCYC 1 cut(s) 822
Fsp4HI GCNGC 1 cut(s) 750
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 598
GluI GCNGC 1 cut(s) 750
HaeIII GGCC 5 cut(s) 76, 252, 772, 781, 797
HapII CCGG 1 cut(s) 753
HhaI GCGC 1 cut(s) 599
Hin1II CATG 3 cut(s) 477, 652, 858
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HinfI GANTC 4 cut(s) 68, 295, 617, 791
HpaII CCGG 1 cut(s) 753
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 7 cut(s) 49, 85, 112, 209, 259, 685, 710
Hpy188III TCNNGA 6 cut(s) 162, 299, 604, 656, 830, 855
HpyAV CCTTC 2 cut(s) 80, 555
HpyCH4III ACNGT 2 cut(s) 519, 706
HpyCH4V TGCA 8 cut(s) 43, 274, 392, 426, 473, 575, 652, 738
HpyF10VI GCNNNNNNNGC 5 cut(s) 276, 280, 423, 542, 778
HpyF3I CTNAG 3 cut(s) 125, 677, 745
Hsp92II CATG 3 cut(s) 477, 652, 858
HspAI GCGC 1 cut(s) 597
KroI GCCGGC 1 cut(s) 752
KroNI GCCGGC 1 cut(s) 754
Kzo9I GATC 3 cut(s) 155, 680, 832
LmnI GCTCC 1 cut(s) 825
LweI GCATC 4 cut(s) 155, 283, 460, 661
MabI ACCWGGT 1 cut(s) 403
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 95, 524
MalI GATC 3 cut(s) 157, 682, 834
MboI GATC 3 cut(s) 155, 680, 832
MboII GAAGA 4 cut(s) 344, 387, 494, 626
MfeI CAATTG 1 cut(s) 91
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 1 cut(s) 822
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 7 cut(s) 56, 91, 226, 495, 576, 583, 759
MluNI TGGCCA 1 cut(s) 76
MlyI GAGTC 2 cut(s) 77, 785
MmeI TCCRAC 3 cut(s) 72, 110, 170
MnlI CCTC 8 cut(s) 22, 25, 132, 175, 322, 600, 783, 824
Mox20I TGGCCA 1 cut(s) 76
Mph1103I ATGCAT 3 cut(s) 45, 475, 654
MreI CGCCGGCG 1 cut(s) 752
MroNI GCCGGC 1 cut(s) 752
MroXI GAANNNNTTC 2 cut(s) 495, 499
MscI TGGCCA 1 cut(s) 76
MseI TTAA 4 cut(s) 216, 449, 579, 699
MslI CAYNNNNRTG 3 cut(s) 163, 733, 803
Msp20I TGGCCA 1 cut(s) 76
MspI CCGG 1 cut(s) 753
MspR9I CCNGG 5 cut(s) 151, 249, 405, 455, 558
MunI CAATTG 1 cut(s) 91
Mva1269I GAATGC 2 cut(s) 43, 599
MvaI CCWGG 5 cut(s) 151, 249, 405, 455, 558
MwoI GCNNNNNNNGC 5 cut(s) 276, 280, 423, 542, 778
NaeI GCCGGC 1 cut(s) 754
NdeII GATC 3 cut(s) 155, 680, 832
NgoMIV GCCGGC 1 cut(s) 752
NlaIII CATG 3 cut(s) 477, 652, 858
NmeAIII GCCGAG 1 cut(s) 773
NsiI ATGCAT 3 cut(s) 45, 475, 654
NspI RCATGY 1 cut(s) 652
PaeR7I CTCGAG 1 cut(s) 815
PagI TCATGA 1 cut(s) 854
PctI GAATGC 2 cut(s) 43, 599
PdiI GCCGGC 1 cut(s) 754
PdmI GAANNNNTTC 2 cut(s) 495, 499
PfeI GAWTC 2 cut(s) 295, 617
PkrI GCNGC 1 cut(s) 751
PleI GAGTC 2 cut(s) 76, 785
PpsI GAGTC 2 cut(s) 76, 785
Psp124BI GAGCTC 1 cut(s) 822
Psp6I CCWGG 5 cut(s) 149, 247, 403, 453, 556
PspGI CCWGG 5 cut(s) 149, 247, 403, 453, 556
PspPI GGNCC 1 cut(s) 143
PspXI VCTCGAGB 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 440, 743
RsaNI GTAC 2 cut(s) 439, 742
RseI CAYNNNNRTG 3 cut(s) 163, 733, 803
SacI GAGCTC 1 cut(s) 822
SaqAI TTAA 4 cut(s) 216, 449, 579, 699
SatI GCNGC 1 cut(s) 750
Sau3AI GATC 3 cut(s) 155, 680, 832
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 2 cut(s) 77, 785
ScrFI CCNGG 5 cut(s) 151, 249, 405, 455, 558
SduI GDGCHC 1 cut(s) 822
SetI ASST 8 cut(s) 204, 244, 409, 437, 551, 559, 743, 822
SexAI ACCWGGT 1 cut(s) 403
SfaNI GCATC 4 cut(s) 155, 283, 460, 661
SfiI GGCCNNNNNGGCC 1 cut(s) 778
Sfr274I CTCGAG 1 cut(s) 815
SgrAI CRCCGGYG 1 cut(s) 752
SinI GGWCC 1 cut(s) 143
SlaI CTCGAG 1 cut(s) 815
SmiMI CAYNNNNRTG 3 cut(s) 163, 733, 803
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 7 cut(s) 56, 91, 226, 495, 576, 583, 759
SsiI CCGC 1 cut(s) 750
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 822
StyD4I CCNGG 5 cut(s) 149, 247, 403, 453, 556
StyI CCWWGG 1 cut(s) 776
TaaI ACNGT 2 cut(s) 519, 706
TaqI TCGA 3 cut(s) 409, 789, 816
TasI AATT 7 cut(s) 56, 91, 226, 495, 576, 583, 759
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 2 cut(s) 295, 617
Tru1I TTAA 4 cut(s) 216, 449, 579, 699
Tru9I TTAA 4 cut(s) 216, 449, 579, 699
TscAI CASTG 2 cut(s) 145, 272
TspDTI ATGAA 1 cut(s) 687
TspRI CASTG 2 cut(s) 145, 272
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 2 cut(s) 56, 495
XceI RCATGY 1 cut(s) 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 815
XmnI GAANNNNTTC 2 cut(s) 495, 499
XspI CTAG 1 cut(s) 372
Zsp2I ATGCAT 3 cut(s) 45, 475, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.