MD12G1177700.v1.1
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003951
Physical Location & Seq
Reverse (-)
25824027 .. 25825696
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1177700.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGCTGCTGCTACTTATTTATTCTTCACTTTGATCATTGCCAGCACTTTGTGCTCGTTCGGATCAGTTCCGAGTATTGAAGCTCAGAAACTTACTCTCTCAGTTTACTATGAAACTCTTTGCCCCCGTTGTGCTACTTTCATCGTCAAAAATCTTGCTCGAATTTTCGACAATGAACTCATCACCATTCTAAATCTCAGGCTGGTTCCTTGGGGAAATGCATCTACAAACAATTCCAAGAACGCCACTTTTTGTCAGCACGGACCAGATGAGTGCAGGCTAAATTCTCTGGAAGCTTGCGCAATTGACGTTTTACATGATGTGAACAAGCATTTTGCTTTGATATACTGCATAGAATTTCTGGCCATCGAGGGAAGGCACAAGGAATGGCAAACTTGTTTCAGCTCATTGGGATTGCCTTTAAAGCCCGTTTTGGATTGCTACGAAAGTGCAAATGCAACAAAGCTTGAACTAAAATATGCGAATGAAACCGCGTACCTCAGTCAGCCTCTTAAGTTCGTTCCATGGCTGCTTCTGAACAATCAACCGATTGGAAATGACTATGAAAATTTCGCTTCCTACGTATGCAAGGCTTACAAAGGTGTGGCCGTGCCGATGGCCTGCGGATCTGTGCATTTGAAGCAAAAGGCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

219

Amino Acids

24.34

Weight (kDa)

6.92

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 33 - 136 3.2e-30 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 301
AccII CGCG 1 cut(s) 494
AciI CCGC 2 cut(s) 492, 624
AclWI GGATC 2 cut(s) 70, 634
AcoI YGGCCR 2 cut(s) 363, 606
AcsI RAATTY 4 cut(s) 162, 283, 356, 568
AdeI CACNNNGTG 1 cut(s) 51
AfaI GTAC 1 cut(s) 497
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 3 cut(s) 80, 470, 640
AluBI AGCT 4 cut(s) 83, 296, 405, 466
AluI AGCT 4 cut(s) 83, 296, 405, 466
Alw21I GWGCWC 1 cut(s) 56
AlwI GGATC 2 cut(s) 70, 634
AoxI GGCC 3 cut(s) 363, 606, 618
ApeKI GCWGC 3 cut(s) 5, 8, 529
ApoI RAATTY 4 cut(s) 162, 283, 356, 568
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 516
BccI CCATC 2 cut(s) 374, 610
BceAI ACGGC 1 cut(s) 593
BclI TGATCA 1 cut(s) 33
BfrI CTTAAG 1 cut(s) 512
BisI GCNGC 3 cut(s) 6, 9, 530
BlsI GCNGC 3 cut(s) 7, 10, 531
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 207
BmsI GCATC 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 583
BsaJI CCNNGG 2 cut(s) 209, 524
Bse3DI GCAATG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 209, 524
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 4 cut(s) 98, 114, 211, 514
BseXI GCAGC 1 cut(s) 516
BsgI GTGCAG 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 494
BshFI GGCC 3 cut(s) 365, 608, 620
BsiHKAI GWGCWC 1 cut(s) 56
BsnI GGCC 3 cut(s) 365, 608, 620
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 3 cut(s) 33, 62, 626
Bsp19I CCATGG 1 cut(s) 524
BspACI CCGC 2 cut(s) 492, 624
BspANI GGCC 3 cut(s) 365, 608, 620
BspCNI CTCAG 4 cut(s) 97, 113, 210, 513
BspFNI CGCG 1 cut(s) 494
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 2 cut(s) 70, 634
BspTI CTTAAG 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 209, 524
BssMI GATC 3 cut(s) 33, 62, 626
BssT1I CCWWGG 2 cut(s) 209, 524
BstAFI CTTAAG 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 583
BstC8I GCNNGC 4 cut(s) 43, 278, 298, 622
BstDEI CTNAG 4 cut(s) 84, 100, 197, 500
BstDSI CCRYGG 1 cut(s) 524
BstFNI CGCG 1 cut(s) 494
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 3 cut(s) 36, 65, 629
BstMBI GATC 3 cut(s) 33, 62, 626
BstMWI GCNNNNNNNGC 3 cut(s) 51, 424, 640
BstSNI TACGTA 1 cut(s) 583
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 3 cut(s) 365, 608, 620
BtgI CCRYGG 1 cut(s) 524
Cac8I GCNNGC 4 cut(s) 43, 278, 298, 622
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 496
CviAII CATG 2 cut(s) 317, 525
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 4 cut(s) 84, 100, 197, 500
DpnI GATC 3 cut(s) 35, 64, 628
DpnII GATC 3 cut(s) 33, 62, 626
DraI TTTAAA 1 cut(s) 423
DraIII CACNNNGTG 1 cut(s) 51
EaeI YGGCCR 2 cut(s) 363, 606
Eco105I TACGTA 1 cut(s) 583
Eco130I CCWWGG 2 cut(s) 209, 524
Eco47I GGWCC 1 cut(s) 263
EcoT14I CCWWGG 2 cut(s) 209, 524
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 209, 524
FaeI CATG 2 cut(s) 320, 528
FaiI YATR 8 cut(s) 111, 318, 346, 353, 480, 526, 564, 586
FatI CATG 2 cut(s) 316, 524
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 6, 9, 530
Fsp4HI GCNGC 3 cut(s) 6, 9, 530
FspI TGCGCA 1 cut(s) 301
GlaI GCGC 1 cut(s) 301
GluI GCNGC 3 cut(s) 6, 9, 530
HaeIII GGCC 3 cut(s) 365, 608, 620
HhaI GCGC 1 cut(s) 302
Hin1II CATG 2 cut(s) 320, 528
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HindIII AAGCTT 2 cut(s) 294, 464
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 106, 325
Hpy188I TCNGA 4 cut(s) 62, 72, 87, 537
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 2 cut(s) 106, 325
HpyAV CCTTC 1 cut(s) 369
HpyCH4IV ACGT 2 cut(s) 309, 582
HpyCH4V TGCA 7 cut(s) 221, 276, 351, 452, 458, 588, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 424, 640
HpyF3I CTNAG 4 cut(s) 84, 100, 197, 500
HpySE526I ACGT 2 cut(s) 309, 582
Hsp92II CATG 2 cut(s) 320, 528
HspAI GCGC 1 cut(s) 300
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 3 cut(s) 33, 62, 626
LpnPI CCDG 8 cut(s) 55, 184, 188, 262, 275, 279, 347, 634
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 1 cut(s) 230
MaeII ACGT 2 cut(s) 309, 582
MalI GATC 3 cut(s) 35, 64, 628
MboI GATC 3 cut(s) 33, 62, 626
MboII GAAGA 1 cut(s) 16
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 626
MhlI GDGCHC 1 cut(s) 56
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 6 cut(s) 162, 232, 283, 303, 356, 568
MluNI TGGCCA 1 cut(s) 365
MnlI CCTC 3 cut(s) 364, 509, 519
Mox20I TGGCCA 1 cut(s) 365
Mph1103I ATGCAT 1 cut(s) 223
MscI TGGCCA 1 cut(s) 365
MseI TTAA 2 cut(s) 422, 513
Msp20I TGGCCA 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 512
MunI CAATTG 1 cut(s) 303
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 3 cut(s) 51, 424, 640
NcoI CCATGG 1 cut(s) 524
NdeII GATC 3 cut(s) 33, 62, 626
NlaIII CATG 2 cut(s) 320, 528
NlaIV GGNNCC 1 cut(s) 207
NsbI TGCGCA 1 cut(s) 301
NsiI ATGCAT 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PkrI GCNGC 3 cut(s) 7, 10, 531
Ppu21I YACGTR 1 cut(s) 583
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 626
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
SaqAI TTAA 2 cut(s) 422, 513
SatI GCNGC 3 cut(s) 6, 9, 530
Sau3AI GATC 3 cut(s) 33, 62, 626
Sau96I GGNCC 1 cut(s) 263
SduI GDGCHC 1 cut(s) 56
SetI ASST 8 cut(s) 85, 298, 312, 407, 468, 501, 585, 604
SfaNI GCATC 1 cut(s) 230
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SnaBI TACGTA 1 cut(s) 583
Sse9I AATT 6 cut(s) 162, 232, 283, 303, 356, 568
SsiI CCGC 2 cut(s) 492, 624
StyI CCWWGG 2 cut(s) 209, 524
TaiI ACGT 2 cut(s) 312, 585
TaqI TCGA 3 cut(s) 160, 168, 369
TasI AATT 6 cut(s) 162, 232, 283, 303, 356, 568
Tru1I TTAA 2 cut(s) 422, 513
Tru9I TTAA 2 cut(s) 422, 513
TseI GCWGC 3 cut(s) 5, 8, 529
TspDTI ATGAA 5 cut(s) 126, 130, 189, 501, 579
TspGWI ACGGA 1 cut(s) 276
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 4 cut(s) 162, 283, 356, 568
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.