Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000666

Physical Location & Seq

Genomic Coordinates Reverse (-)

25706493 .. 25712189

Gene Structure 5697 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1166900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2082 bp

ATGGGGGCAGGAAGGAAAACAAAAACCTTTACTGCGCCTCCTCAAGATCTGGTGACCTACTCTGCATCCATGAGAAATTTGAGCAAAAATCACCTTGGTGGAGTCGTATTTGGTTGCACCAAAACCACAATGGAAGAATGTCTATATAAACAACTCTTTGGCTTACCAGGTGCACACTTTTCATATGTAAAGAATATCACTCCTGGTTTGCTGCTTTTTCTCTTCAACTATAACGATAGGAAACTACATGGTATCTTTGAGGCTGCAAGCTGTGGCCAAATGAATATTAACCCGTATGGATGGACCACTGATGGTTCAGAGAAAACACAATTTCCTGCTCAGGTTCAAATCCGTGTCCGGCTGCAGTGCCGACCAGTGCCCGAAAGTCAGTTTAAACCAATACTTGTTGACAACTACTACAGTCAGCGTCATTTCTGGTTTGAGCTTGACCACGCTCAAACAAGTAGGGTGATGTCTTTGTTCGCAACTGCTGGAATCGCTTCAAGTACATCTGTAGGTTCTTTACCACGGAATAAAATTAAGTGGAGTGATACGTTTCAAGCCCGTACCTCAACTGGCACATTAGAATGGTTTAAACCACTTGCTTCAGCAGATTCCACAAAGTTAGTTGAAAGAAATGATCCATTCAACGCCCAAATAGAAATCATGGAGGCTGAACAATCGGAGGAAGAACTCATATTCATGAAACTGAAAGAGTTAGCACTCCAATCCAGCCTCAAGAGTAGTGAAGGTGAAGACATATCTTTGTCAGGAGTTGCTGAAGATCATCATGTCTTGAATGAATTGACCTTGGACGACAATGAGAATCCAACGGAGCCATCGGGTTTGGAGGAGAAGAGGGGAGGAAATCCTCACTCCTCATCTGAGGAGCAGAAGGTAGAAAGTCCTCAATTCTCATCTGAGGAGAAGAGGGGAGAAAGTCCTCAATCCTCATCTGAGCAGAGGAGTGGAGAGAGTGCTCAATCCTTGTCTGAGTTTCAGGCAATCATTACAAAGTTGAATCAAGAGATGGAGGAGCTCAGGGCTTATAAGACAGAGTTCACAGAACAATCTAAAAAGTTTGGTTATTTGGAGCATAAGCTGGCAGAGACGGAAATTCGATGCTTAAAATTTGGTCTACAGTTGGAATCAGGGTCTACACCTTCCAAGGCACATACTGATGAGAAGGATACTGAATCATGCAATGTGCTGCATTATGACCCCAATGACTCGATGTATCTAGTTGGTGGATATGATGGTGAATCATGTTTGTCGGCATTTGATGCTTATTATCCTTCTCAAGATATGATAAAACCTCTGAGAACAATTAGTTCAGTTCGTTCATATGCCTCTGTTGCACAATTGAATGGTGATCTTTATGTAATTGGTGGTGGCAATGGTCATGTGTGGTATGATACAGTTGAATCGTACAGCCCTGGTACTGATGAGTGGAAGCAGTGCCCTTCTCTGAGAGAGAAAAAGGGTAGCTTAGCTGCAGCCACCACAAACAGCAAAATTTTTGCAATGGGTGGTGGGAATGGAGTTGATTGCTTTTCAGATGTTGAGATGCTTGATTTAGATGTTGGACGATGGATCCGTACACAGTCAATGTCACAAAAGCGTATGGGTCTTGCTGCAGTAGAACTCAATGGTGTGCTTTATGCCACTGGTGGATTTGATGGGACTAGTTATTTGAACTCTGTTGATAGATTTGACCCCAGAGAACACAAGTGGACCAAAATTGCCTCTATGCATTCGAAAAGGGGTTGCCATTCATTGGTTGTTCTGAATGAAAAAATATATGCTCTGGGAGGCTTTGATGGAGATTCAATTATCCAGAGTGTGGAAAGTTTTGATCCACGGCTCGGGTCATGGATGCTGGCCGAACCAATGTATAACTCCAGGGGGTATTCAACGGCTGCCGTTATCAAGGATACCATATATGTAATCGGAGGGATGAAATCTGACGAATGCATAGCAGAGACAGTTGAATACTACAAGGAGGGGCAAGGTTGGCAGGAAATGGCGATGACGGCCATCGGTAAAAGGTGTTTCATGTCCGCCGTTGCTTATTCGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0008150 GO:0008582 GO:0040008 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044087 GO:0044424 GO:0044464 GO:0045886 GO:0045926 GO:0048519 GO:0048523 GO:0048638 GO:0048640 GO:0050789 GO:0050793 GO:0050794 GO:0050803 GO:0050807 GO:0051093 GO:0051128 GO:0051129 GO:0051239 GO:0051241 GO:0051960 GO:0051961 GO:0051963 GO:0051964 GO:0065007 GO:0065008 GO:1904396 GO:1904397 GO:1905809 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dev_Cell_Death Kelch_1 Kelch_5

Protein Analysis

694

Amino Acids

77.07

Weight (kDa)

5.23

Isoelectric Point (pI)

53.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dev_Cell_Death	PF10539	33 - 161	1.3e-49	Development and cell death domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	406 - 539	3.6e-12	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	406 - 566	1.3e-07	FBX42, beta-propeller domain
Kelch_1	PF01344	447 - 490	4.8e-13	Kelch motif
Beta-prop_Calicin	PF13964	481 - 626	6.9e-14	Calicin, beta-propeller domain
Kelch_FKB95	PF25210	488 - 582	4.6e-08	FKB95, Kelch-repeats domain
Kelch_1	PF01344	498 - 538	2e-06	Kelch motif
Kelch_KLHDC2_KLHL20_DRC7	PF24681	499 - 678	1.5e-18	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	502 - 657	4.8e-14	FBX42, beta-propeller domain
Kelch_1	PF01344	541 - 584	4.2e-14	Kelch motif
Beta-prop_ATRN-LZTR1	PF24981	566 - 676	3.9e-07	Attractin/LZTR1 beta-propeller
Kelch_FKB95	PF25210	581 - 673	5.4e-10	FKB95, Kelch-repeats domain
Kelch_1	PF01344	588 - 631	4.9e-11	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01660 AT5G01660 AT5G01660
fragaria_vesca	FvH4_6g10590 FvH4_6g10591 FvH4_6g10591 FvH4_6g10591
malus_domestica	MD04G1166900.v1.1 MD12G1180200.v1.1 MD12G1180500.v1.1
prunus_persica	Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1
pyrus_communis	pycom04g14830 pycom12g16990
rosa_chinensis	RchiOBHm_Chr2g0145311 RchiOBHm_Chr3g0461571 RchiOBHm_Chr3g0461581
rosa_laevigata	RLG00000024916 RLG00000024917
rosa_multiflora	Rmu_sc0000790.1_g000001 Rmu_sc0003979.1_g000002 Rmu_sc0006964.1_g000002 Rmu_sc0010327.1_g000003 Rmu_ssc0000193.1_g000005 Rmu_ssc0000193.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330190 Rroxscaffold_6G00418120 Rroxscaffold_6G00418130 Rroxscaffold_6G00418490 Rroxscaffold_6G00418500
rosa_rugosa	Rorug01G0017200 Rorug01G0017900 Rorug03G0051900 Rorug03G0052000 Rorug03G0052000 Rorug03G0052100
rosa_samantha	Rh1CG027200 Rh1CG027400 Rh1CG027500 Rh1CG027700 Rh1CG073300 Rh1CG101500 Rh1CG101600 Rh1DG042300 Rh1DG042800 Rh3AG110200 Rh3AG110300 Rh3BG113400 Rh3BG113500 Rh3CG115300 Rh3CG115400 Rh3DG115100 Rh3DG115200 Rh5AG167800 Rh5BG556300 Rh5CG579000 Rh6AG136600 Rh7DG359300
rosa_wichuraiana	Rw1G002200 Rw1G002230 Rw3G009320 Rw3G009330 Rw7G020340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1050
AccB7I	CCANNNNNTGG	2 cut(s)	1777, 1843
AccI	GTMKAC	2 cut(s)	1138, 1157
AciI	CCGC	1 cut(s)	2061
AclWI	GGATC	4 cut(s)	635, 1588, 1601, 1850
AcoI	YGGCCR	3 cut(s)	274, 1881, 2034
AcsI	RAATTY	4 cut(s)	76, 1116, 1130, 1515
AcuI	CTGAAG	2 cut(s)	591, 801
AfaI	GTAC	5 cut(s)	508, 568, 1430, 1441, 1600
AfiI	CCNNNNNNNGG	3 cut(s)	1777, 1843, 1865
AhlI	ACTAGT	1 cut(s)	1685
AjnI	CCWGG	4 cut(s)	166, 202, 1435, 1901
AleI	CACNNNNGTG	1 cut(s)	96
AluBI	AGCT	6 cut(s)	270, 445, 1039, 1102, 1488, 1493
AluI	AGCT	6 cut(s)	270, 445, 1039, 1102, 1488, 1493
Alw21I	GWGCWC	3 cut(s)	175, 982, 1041
Alw26I	GTCTC	2 cut(s)	1103, 1976
Alw44I	GTGCAC	1 cut(s)	171
AlwI	GGATC	4 cut(s)	635, 1588, 1601, 1850
Ama87I	CYCGRG	1 cut(s)	1865
AoxI	GGCC	3 cut(s)	274, 1881, 2034
ApaLI	GTGCAC	1 cut(s)	171
ApeKI	GCWGC	8 cut(s)	211, 263, 361, 1210, 1493, 1496, 1634, 1919
ApoI	RAATTY	4 cut(s)	76, 1116, 1130, 1515
ArsI	GACNNNNNNTTYG	2 cut(s)	1675, 1707
Asp700I	GAANNNNTTC	1 cut(s)	499
AspLEI	GCGC	1 cut(s)	37
AspS9I	GGNCC	2 cut(s)	303, 1734
AsuHPI	GGTGA	6 cut(s)	64, 83, 481, 764, 1271, 1382
AsuII	TTCGAA	1 cut(s)	1757
AvaI	CYCGRG	1 cut(s)	1865
AvaII	GGWCC	2 cut(s)	303, 1734
BaeGI	GKGCMC	3 cut(s)	175, 381, 1463
BalI	TGGCCA	1 cut(s)	276
BamHI	GGATCC	1 cut(s)	1593
BanII	GRGCYC	1 cut(s)	1041
BbsI	GAAGAC	1 cut(s)	762
Bbv12I	GWGCWC	3 cut(s)	175, 982, 1041
BbvI	GCAGC	8 cut(s)	198, 250, 348, 1197, 1480, 1508, 1621, 1906
BccI	CCATC	9 cut(s)	294, 305, 847, 1024, 1250, 1584, 1673, 1814, 2045
BceAI	ACGGC	5 cut(s)	1877, 1907, 1932, 2048, 2049
BciT130I	CCWGG	4 cut(s)	168, 204, 1437, 1903
BciVI	GTATCC	2 cut(s)	1183, 1927
BcoDI	GTCTC	2 cut(s)	1103, 1976
BcuI	ACTAGT	1 cut(s)	1685
BfaI	CTAG	2 cut(s)	1241, 1686
BfmI	CTRYAG	6 cut(s)	362, 418, 513, 1139, 1494, 1635
BfuI	GTATCC	2 cut(s)	1183, 1927
BglII	AGATCT	1 cut(s)	46
BisI	GCNGC	8 cut(s)	212, 264, 362, 1211, 1494, 1497, 1635, 1920
BlpI	GCTNAGC	1 cut(s)	1489
BlsI	GCNGC	8 cut(s)	213, 265, 363, 1212, 1495, 1498, 1636, 1921
Bme1390I	CCNGG	4 cut(s)	168, 204, 1437, 1903
Bme18I	GGWCC	2 cut(s)	303, 1734
BmeT110I	CYCGRG	1 cut(s)	1865
BmgT120I	GGNCC	2 cut(s)	303, 1734
BmiI	GGNNCC	2 cut(s)	837, 1595
BmrFI	CCNGG	4 cut(s)	168, 204, 1437, 1903
BmsI	GCATC	5 cut(s)	74, 1112, 1273, 1557, 1866
BpiI	GAAGAC	1 cut(s)	762
BpmI	CTGGAG	1 cut(s)	1885
Bpu10I	CCTNAGC	2 cut(s)	339, 1040
Bpu1102I	GCTNAGC	1 cut(s)	1489
Bpu14I	TTCGAA	1 cut(s)	1757
BpuEI	CTTGAG	3 cut(s)	27, 722, 1284
BsaJI	CCNNGG	7 cut(s)	94, 527, 810, 1167, 1435, 1859, 1902
BsaXI	ACNNNNNCTCC	2 cut(s)	22, 52
Bsc4I	CCNNNNNNNGG	3 cut(s)	1777, 1843, 1865
Bse1I	ACTGG	3 cut(s)	374, 580, 1672
Bse3DI	GCAATG	3 cut(s)	1210, 1402, 1530
BseBI	CCWGG	4 cut(s)	168, 204, 1437, 1903
BseDI	CCNNGG	7 cut(s)	94, 527, 810, 1167, 1435, 1859, 1902
BseGI	GGATG	4 cut(s)	65, 305, 1881, 1962
BseLI	CCNNNNNNNGG	3 cut(s)	1777, 1843, 1865
BseMI	GCAATG	3 cut(s)	1210, 1402, 1530
BseMII	CTCAG	8 cut(s)	353, 876, 912, 948, 984, 1054, 1310, 1460
BseNI	ACTGG	3 cut(s)	374, 580, 1672
BseRI	GAGGAG	7 cut(s)	30, 866, 868, 902, 938, 979, 1049
BseSI	GKGCMC	3 cut(s)	175, 381, 1463
BseXI	GCAGC	8 cut(s)	198, 250, 348, 1197, 1480, 1508, 1621, 1906
BshFI	GGCC	3 cut(s)	276, 1883, 2036
BsiHKAI	GWGCWC	3 cut(s)	175, 982, 1041
BsiHKCI	CYCGRG	1 cut(s)	1865
BsiSI	CCGG	1 cut(s)	358
BslFI	GGGAC	1 cut(s)	1696
BslI	CCNNNNNNNGG	3 cut(s)	1777, 1843, 1865
BsmAI	GTCTC	2 cut(s)	1103, 1976
BsmBI	CGTCTC	1 cut(s)	1103
BsmFI	GGGAC	1 cut(s)	1696
BsmI	GAATGC	2 cut(s)	1753, 1976
BsnI	GGCC	3 cut(s)	276, 1883, 2036
BsoBI	CYCGRG	1 cut(s)	1865
Bsp119I	TTCGAA	1 cut(s)	1757
Bsp1286I	GDGCHC	5 cut(s)	175, 381, 982, 1041, 1463
Bsp143I	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
Bsp1720I	GCTNAGC	1 cut(s)	1489
BspACI	CCGC	1 cut(s)	2061
BspANI	GGCC	3 cut(s)	276, 1883, 2036
BspCNI	CTCAG	8 cut(s)	352, 877, 913, 949, 985, 1053, 1311, 1461
BspHI	TCATGA	1 cut(s)	702
BspLI	GGNNCC	2 cut(s)	837, 1595
BspMAI	CTGCAG	3 cut(s)	366, 1498, 1639
BspPI	GGATC	4 cut(s)	635, 1588, 1601, 1850
BspT104I	TTCGAA	1 cut(s)	1757
BsrDI	GCAATG	3 cut(s)	1210, 1402, 1530
BsrI	ACTGG	3 cut(s)	374, 580, 1672
BssECI	CCNNGG	7 cut(s)	94, 527, 810, 1167, 1435, 1859, 1902
BssMI	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
BssT1I	CCWWGG	3 cut(s)	94, 810, 1167
Bst2UI	CCWGG	4 cut(s)	168, 204, 1437, 1903
Bst4CI	ACNGT	5 cut(s)	422, 1143, 1420, 1605, 1987
Bst6I	CTCTTC	3 cut(s)	227, 851, 923
BstAPI	GCANNNNNTGC	1 cut(s)	1283
BstBI	TTCGAA	1 cut(s)	1757
BstC8I	GCNNGC	3 cut(s)	268, 1104, 1881
BstDEI	CTNAG	9 cut(s)	339, 885, 921, 957, 993, 1040, 1319, 1469, 1489
BstDSI	CCRYGG	2 cut(s)	527, 1859
BstEII	GGTNACC	1 cut(s)	52
BstF5I	GGATG	4 cut(s)	65, 305, 1881, 1962
BstHHI	GCGC	1 cut(s)	37
BstKTI	GATC	6 cut(s)	49, 643, 787, 1375, 1596, 1858
BstMAI	GTCTC	2 cut(s)	1103, 1976
BstMBI	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
BstMWI	GCNNNNNNNGC	5 cut(s)	497, 1283, 1355, 2014, 2033
BstNI	CCWGG	4 cut(s)	168, 204, 1437, 1903
BstPI	GGTNACC	1 cut(s)	52
BstSCI	CCNGG	4 cut(s)	166, 202, 1435, 1901
BstSFI	CTRYAG	6 cut(s)	362, 418, 513, 1139, 1494, 1635
BstSLI	GKGCMC	3 cut(s)	175, 381, 1463
BstV1I	GCAGC	8 cut(s)	198, 250, 348, 1197, 1480, 1508, 1621, 1906
BstV2I	GAAGAC	1 cut(s)	762
BstX2I	RGATCY	2 cut(s)	46, 1593
BstYI	RGATCY	2 cut(s)	46, 1593
BsuI	GTATCC	2 cut(s)	1183, 1927
BsuRI	GGCC	3 cut(s)	276, 1883, 2036
BtgI	CCRYGG	2 cut(s)	527, 1859
BtgZI	GCGATG	1 cut(s)	2042
BtsCI	GGATG	4 cut(s)	65, 305, 1881, 1962
BtsI	GCAGTG	2 cut(s)	371, 1463
BtsIMutI	CAGTG	5 cut(s)	306, 371, 381, 1463, 1665
Cac8I	GCNNGC	3 cut(s)	268, 1104, 1881
CciI	TCATGA	1 cut(s)	702
CfoI	GCGC	1 cut(s)	37
Cfr13I	GGNCC	2 cut(s)	303, 1734
CseI	GACGC	1 cut(s)	416
CsiI	ACCWGGT	1 cut(s)	166
Csp6I	GTAC	5 cut(s)	507, 567, 1429, 1440, 1599
CviQI	GTAC	5 cut(s)	507, 567, 1429, 1440, 1599
DdeI	CTNAG	9 cut(s)	339, 885, 921, 957, 993, 1040, 1319, 1469, 1489
DpnI	GATC	6 cut(s)	48, 642, 786, 1374, 1595, 1857
DpnII	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
DraI	TTTAAA	2 cut(s)	394, 595
EaeI	YGGCCR	3 cut(s)	274, 1881, 2034
Eam1104I	CTCTTC	3 cut(s)	227, 851, 923
EarI	CTCTTC	3 cut(s)	227, 851, 923
EciI	GGCGGA	1 cut(s)	2050
Ecl136II	GAGCTC	1 cut(s)	1039
Eco130I	CCWWGG	3 cut(s)	94, 810, 1167
Eco24I	GRGCYC	1 cut(s)	1041
Eco47I	GGWCC	2 cut(s)	303, 1734
Eco53kI	GAGCTC	1 cut(s)	1039
Eco57I	CTGAAG	2 cut(s)	591, 801
Eco88I	CYCGRG	1 cut(s)	1865
Eco91I	GGTNACC	1 cut(s)	52
EcoICRI	GAGCTC	1 cut(s)	1039
EcoO65I	GGTNACC	1 cut(s)	52
EcoRII	CCWGG	4 cut(s)	166, 202, 1435, 1901
EcoT14I	CCWWGG	3 cut(s)	94, 810, 1167
EcoT22I	ATGCAT	2 cut(s)	1755, 1976
EcoT38I	GRGCYC	1 cut(s)	1041
ErhI	CCWWGG	3 cut(s)	94, 810, 1167
Esp3I	CGTCTC	1 cut(s)	1103
FalI	AAGNNNNNCTT	2 cut(s)	1472, 1504
FaqI	GGGAC	1 cut(s)	1696
FauNDI	CATATG	2 cut(s)	184, 1345
FblI	GTMKAC	2 cut(s)	1138, 1157
Fnu4HI	GCNGC	8 cut(s)	212, 264, 362, 1211, 1494, 1497, 1635, 1920
FokI	GGATG	4 cut(s)	52, 312, 1888, 1969
FriOI	GRGCYC	1 cut(s)	1041
Fsp4HI	GCNGC	8 cut(s)	212, 264, 362, 1211, 1494, 1497, 1635, 1920
FspBI	CTAG	2 cut(s)	1241, 1686
GlaI	GCGC	1 cut(s)	36
GluI	GCNGC	8 cut(s)	212, 264, 362, 1211, 1494, 1497, 1635, 1920
GsuI	CTGGAG	1 cut(s)	1885
HaeIII	GGCC	3 cut(s)	276, 1883, 2036
HapII	CCGG	1 cut(s)	358
HgaI	GACGC	1 cut(s)	416
HhaI	GCGC	1 cut(s)	37
Hin6I	GCGC	1 cut(s)	35
HinP1I	GCGC	1 cut(s)	35
HincII	GTYRAC	1 cut(s)	409
HindII	GTYRAC	1 cut(s)	409
HpaII	CCGG	1 cut(s)	358
HphI	GGTGA	6 cut(s)	64, 83, 481, 764, 1271, 1382
Hpy166II	GTNNAC	7 cut(s)	173, 409, 1062, 1139, 1158, 1601, 1734
Hpy188III	TCNNGA	8 cut(s)	44, 703, 739, 771, 796, 1025, 1301, 1837
Hpy8I	GTNNAC	7 cut(s)	173, 409, 1062, 1139, 1158, 1601, 1734
HpyAV	CCTTC	7 cut(s)	6, 743, 889, 1173, 1180, 1305, 1473
HpyCH4III	ACNGT	5 cut(s)	422, 1143, 1420, 1605, 1987
HpyCH4IV	ACGT	1 cut(s)	554
HpyF10VI	GCNNNNNNNGC	5 cut(s)	497, 1283, 1355, 2014, 2033
HpyF3I	CTNAG	9 cut(s)	339, 885, 921, 957, 993, 1040, 1319, 1469, 1489
HpySE526I	ACGT	1 cut(s)	554
HspAI	GCGC	1 cut(s)	35
Kzo9I	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
LmnI	GCTCC	4 cut(s)	835, 889, 1036, 1093
Lsp1109I	GCAGC	8 cut(s)	198, 250, 348, 1197, 1480, 1508, 1621, 1906
LweI	GCATC	5 cut(s)	74, 1112, 1273, 1557, 1866
MabI	ACCWGGT	1 cut(s)	166
MaeI	CTAG	2 cut(s)	1241, 1686
MaeII	ACGT	1 cut(s)	554
MaeIII	GTNAC	2 cut(s)	52, 1611
MalI	GATC	6 cut(s)	48, 642, 786, 1374, 1595, 1857
MboI	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
MboII	GAAGA	7 cut(s)	146, 214, 701, 767, 794, 868, 940
MfeI	CAATTG	1 cut(s)	1361
MflI	RGATCY	2 cut(s)	46, 1593
MhlI	GDGCHC	5 cut(s)	175, 381, 982, 1041, 1463
MlsI	TGGCCA	1 cut(s)	276
MluNI	TGGCCA	1 cut(s)	276
MlyI	GAGTC	2 cut(s)	111, 1223
MmeI	TCCRAC	3 cut(s)	854, 1125, 1564
Mox20I	TGGCCA	1 cut(s)	276
Mph1103I	ATGCAT	2 cut(s)	1755, 1976
MroXI	GAANNNNTTC	1 cut(s)	499
MscI	TGGCCA	1 cut(s)	276
MseI	TTAA	5 cut(s)	288, 393, 540, 594, 1127
MslI	CAYNNNNRTG	4 cut(s)	96, 586, 701, 1179
Msp20I	TGGCCA	1 cut(s)	276
MspI	CCGG	1 cut(s)	358
MspR9I	CCNGG	4 cut(s)	168, 204, 1437, 1903
MssI	GTTTAAAC	2 cut(s)	394, 595
MunI	CAATTG	1 cut(s)	1361
Mva1269I	GAATGC	2 cut(s)	1753, 1976
MvaI	CCWGG	4 cut(s)	168, 204, 1437, 1903
MwoI	GCNNNNNNNGC	5 cut(s)	497, 1283, 1355, 2014, 2033
NdeI	CATATG	2 cut(s)	184, 1345
NdeII	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
NlaIV	GGNNCC	2 cut(s)	837, 1595
NmuCI	GTSAC	2 cut(s)	52, 1611
NsiI	ATGCAT	2 cut(s)	1755, 1976
NspV	TTCGAA	1 cut(s)	1757
OliI	CACNNNNGTG	1 cut(s)	96
PagI	TCATGA	1 cut(s)	702
PcsI	WCGNNNNNNNCGW	1 cut(s)	1594
PctI	GAATGC	2 cut(s)	1753, 1976
PdmI	GAANNNNTTC	1 cut(s)	499
PfeI	GAWTC	9 cut(s)	495, 614, 826, 1021, 1148, 1196, 1262, 1424, 1826
PflMI	CCANNNNNTGG	2 cut(s)	1777, 1843
PkrI	GCNGC	8 cut(s)	213, 265, 363, 1212, 1495, 1498, 1636, 1921
PleI	GAGTC	2 cut(s)	110, 1223
PmeI	GTTTAAAC	2 cut(s)	394, 595
PpsI	GAGTC	2 cut(s)	110, 1223
PsiI	TTATAA	1 cut(s)	1050
Psp124BI	GAGCTC	1 cut(s)	1041
Psp6I	CCWGG	4 cut(s)	166, 202, 1435, 1901
PspEI	GGTNACC	1 cut(s)	52
PspGI	CCWGG	4 cut(s)	166, 202, 1435, 1901
PspN4I	GGNNCC	2 cut(s)	837, 1595
PspPI	GGNCC	2 cut(s)	303, 1734
PstI	CTGCAG	3 cut(s)	366, 1498, 1639
PsuI	RGATCY	2 cut(s)	46, 1593
RsaI	GTAC	5 cut(s)	508, 568, 1430, 1441, 1600
RsaNI	GTAC	5 cut(s)	507, 567, 1429, 1440, 1599
RseI	CAYNNNNRTG	4 cut(s)	96, 586, 701, 1179
SacI	GAGCTC	1 cut(s)	1041
SaqAI	TTAA	5 cut(s)	288, 393, 540, 594, 1127
SatI	GCNGC	8 cut(s)	212, 264, 362, 1211, 1494, 1497, 1635, 1920
Sau3AI	GATC	6 cut(s)	46, 640, 784, 1372, 1593, 1855
Sau96I	GGNCC	2 cut(s)	303, 1734
SchI	GAGTC	2 cut(s)	111, 1223
ScrFI	CCNGG	4 cut(s)	168, 204, 1437, 1903
SduI	GDGCHC	5 cut(s)	175, 381, 982, 1041, 1463
SexAI	ACCWGGT	1 cut(s)	166
SfaNI	GCATC	5 cut(s)	74, 1112, 1273, 1557, 1866
SfcI	CTRYAG	6 cut(s)	362, 418, 513, 1139, 1494, 1635
SfuI	TTCGAA	1 cut(s)	1757
SinI	GGWCC	2 cut(s)	303, 1734
SmiMI	CAYNNNNRTG	4 cut(s)	96, 586, 701, 1179
SmlI	CTYRAG	3 cut(s)	42, 737, 1299
SmoI	CTYRAG	3 cut(s)	42, 737, 1299
SpeI	ACTAGT	1 cut(s)	1685
SsiI	CCGC	1 cut(s)	2061
SspI	AATATT	1 cut(s)	286
SspMI	CTAG	2 cut(s)	1241, 1686
SstI	GAGCTC	1 cut(s)	1041
StyD4I	CCNGG	4 cut(s)	166, 202, 1435, 1901
StyI	CCWWGG	3 cut(s)	94, 810, 1167
TaaI	ACNGT	5 cut(s)	422, 1143, 1420, 1605, 1987
TaiI	ACGT	1 cut(s)	557
TaqI	TCGA	4 cut(s)	1120, 1232, 1757, 2075
TatI	WGTACW	1 cut(s)	506
TfiI	GAWTC	9 cut(s)	495, 614, 826, 1021, 1148, 1196, 1262, 1424, 1826
Tru1I	TTAA	5 cut(s)	288, 393, 540, 594, 1127
Tru9I	TTAA	5 cut(s)	288, 393, 540, 594, 1127
TscAI	CASTG	5 cut(s)	313, 371, 381, 1463, 1672
TseFI	GTSAC	2 cut(s)	52, 1611
TseI	GCWGC	8 cut(s)	211, 263, 361, 1210, 1493, 1496, 1634, 1919
Tsp45I	GTSAC	2 cut(s)	52, 1611
TspGWI	ACGGA	5 cut(s)	341, 544, 848, 1127, 1586
TspRI	CASTG	5 cut(s)	313, 371, 381, 1463, 1672
Van91I	CCANNNNNTGG	2 cut(s)	1777, 1843
VneI	GTGCAC	1 cut(s)	171
VpaK11BI	GGWCC	2 cut(s)	303, 1734
XapI	RAATTY	4 cut(s)	76, 1116, 1130, 1515
XcmI	CCANNNNNNNNNTGG	1 cut(s)	127
XmiI	GTMKAC	2 cut(s)	1138, 1157
XmnI	GAANNNNTTC	1 cut(s)	499
XspI	CTAG	2 cut(s)	1241, 1686
Zsp2I	ATGCAT	2 cut(s)	1755, 1976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.