Rorug01G0017900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000666
Physical Location & Seq
Reverse (-)
2955133 .. 2968979
13847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0017900.1

Sequence Viewer

Length: 4953 bp
ATGGAGGAGAGCAACAAAAAAATTCACAGTGTTGATAGAATTGACATTCCTAAAGGATGCTCATTGCAGTTACAAAAACTTTTCACAGATGTAGCCCTTCATGTTGGAGGTTTCTTCTTAATGTATATCCTGTGTCATCTTTCGCACTACCTGCTAAGGCCCTATTCACAGCCTCGCATTACCTCTGATACCTTTGTAGGACTAGTCATCGGAAACATAGGGTTCATACGCAATATTCTATCAAAGCGAGTATTGCAGTACATAGTTGATTTTGGAATGATTGGCTATATGTTTGTGCTGGGAATAGAAATGGATCCATATGTGCTTGTTAGAGCACCAACCAAAGACGCAAAAGTAGCCTATGCTGGTATGCTCTTCACATTCGCCCTGGCCTGTGGAATAACCCCATTTCTGAACTATACAGACGGTTTTAAGATTGACTTCACTCTCTCCCTCTCCATGGTCCTCTCTAGCACGGCTTCGCCTGTACTGACACGTATGATAACAAATCTCAAAATAGGCAAGTCTGACATAGGCCAGCTTGTCATTGCTACTGGAATGCATTCTGATTTTATAGCCACTCTTCTCCTCTCCCTTGGCTATGTCATTTGGCCTGTTCAAGTTGAAGATAAAAAGCCCAATGTTCGCAGATCCCTTCAGATGGGTGCTGCATTGTTGGTCCAGACAATTTTTACAGCCATTGTTTCACCAGTCGTCATGAACTGGGTCAACAATGAAAACCCCGAAGGCAAACCCATCAAAGGTTCACACCTAGTCCTCTCTATGGCTTTCATGGCCTTTGTTGTGGCTTGCCCATCATTTTGGGGGTACAGTGCTATTTTGAGTGCATTTATGGCAGGAATCTTTCTACCAAGGGAAGGAAGAGTATCAAAGTGGGTAATAGGCAAGATCAATTACTTGTTAAGTACCATATTCTATCCCATATTTTTCTTTTGGATGGGGTATGAAGCTTATTTTATGGAGTTTCAACCTCTTGACTGGATGACATGGGCAAGGTTATTTGTAGTTTTTGCTATAGCAACAATCGGAAAAGTTGCTGGAACAGTGGTCTCTGGAGCGTTGTTGGGGTTTAACTGGCCTGAATCTGTTGCACTTGGGCTTCTTCTCACTACCAAAGGCCATTTTCATATATACTTGGCTATTGCTGCAAAATCGTATGGGAAAACCACTACTTCAACCAGCACTGTGATGGTAATAGCTATTTTTTTTACTGTGGTGCACGCCCCATCAGTTGTGGCTCAAATTATTAAGCGTGCAAGGAGACGGGCACCCACTCATCGGATGTCACTTCAGTTGCTTGAACCTTCAAGCGAACTCCGCATATTGCTATGCCTTCATGGGCCACAAAATTTGCATACAACCATTAACTTCATTGAGATATGTAGAGGGACAGCAGACCCTGGAATCGTGGTATTTGTAACAGACATGATAGAACTCACAGACCAAATAGCAGCCACACTAGTTAAAGAAGAAGGAGTGAACACAATGACTGTGACTGACAAGGCAGTCACAGACAGGAGAGATCAAGTCACTGCTGCAGTTCAAGCCTATGTAGAGGAGGATGAAACTGGTATTACACTTGGACGATTGCTTGCACTTTCGACTTTCAATGGCATGCCCCAAGATATATGCATTTTGGCTGAGGACTCGATGGTGGCCCTCATCATATTGCCGTTTCACAAGACACAAGGTGCAGATGGGACATTAGATGGCGGCCATTCTGGTTTCAGATACGTGAACCGTAAGGTCCTCAGAAATGCTCCATGCTCCGTAGGAATTCTTGTAGACAGAGGTTTTGGACTGATTGAAAAAATATCAAGATCTCATGATCATTCTATCGATATGGCAGTCATCTTCATTGGTGGAAAAGATGATAGAGAAGCACTTGCTTATGCCGGTCATGTAGCACAACATCCTGGTGTAAAACTCACCATCTTAAGATTTTTGGTAGACAGTAGCTCGGATGCCTCAAGTAGACCTAGCCATTATCAGGTAAGTCGTGCTCTACAAGAAGAGGAAATGAAGCTTGATGATGAGTGCTTTGCTAAGTTCTACGAGAGACATGTTGCAATTGGGCAAGTTGCTTACATGGAGAAGCATCTTGCCAATTCTTCTGAGACCTATAATACCTTGATGACATTGGATGGGCAGTACTCACTTATTATAGTGGGAAGAGGGGAAAGGGTGAATACAGTTTTGACATTTGGGATGAATGACTGGCAGAAATGTCCGGAGTTGGGTCCGATCGGTGATGTTCTTTCAGGTTCTGAATTTTCAGTCAGAACCTCAGTTTTGATCATCCAACAAAACAGTGGAAAAGGAGAACTAGATGGACTTGATGATGACTTTTCTGTCATCCGGCGAATCTGCTTCTGTCCGCTAGGGTTTCCTCGATCCCTCGCCACCGCGAATTCCAAGGCGTGCTTCCTCGACGAGGTCATCAAGGTCCATGACTTTCTCAGCGATCCGTCCGGAATTAGGGCTCGCGGCGACGACGCCAAAACCGTCCAGGTCGCCGTTCCCAAAGTCGCGCCGCCGCCTCCTCCGCCGCCGACGGCTCAGCCTGTTCCGCCGTCGGTGGCCGGAGGAGATGCCGCGGTGACGGCGCCGGTCGATGATGAGTCGGCGGCTGCGGCCTCCGCGCAGGCCAAGCGCGCTGCTCTCCAGCGCAAGGCTGCCGCGGATATGGTGGCCGCTGAGGACTTCGCTCGCCGCTTTGAGTCTGGTGATTTGTCGGACACCTCCAGAGGAGTTGTGGGAGAAGAGCAGGGTCAGTCCAATGTTATGTGTCGGATATGCTTTTGTGGTGAAAATGAAGGAAGTCAGAGAGCAAGAAGGATGCTTCCATGTAAAACCTGTGGCAAAAAGTACCATAGGAACTGCTTGAAAACTTGGTCTCAACACAGAGATCTATTTCACTGGAGTTCATGGGCATGCCCCTCGTGCCGAATTTGTGAGGTATGTCGAAGAACAGGAGATCCAAACAAACTTATGTTCTGCAAAAGGTGTGATGGTGCTTACCACTGTTACTGCCAACATCCTTCACATAAGAATGTTTCTGCTGGAGTTTATGTGTGCCCAAAACATACAAAGTGTCACAGCTGTGGGTCAAACGTGCCAGGAAATGGTTTGAGTGTAAGGTGGTTTTTGGGGTACACCTGCTGTGATGCATGTGGACGATTATTTGTTAAGGGGAATTATTGCCCTGTTTGCTTGAAGGTTTATAGAGATTCAGAATCAACACCTATGGTCTGCTGTGATATTTGCCAGCGTTGGGTACATTGCCATTGTGATGGGATCAGTGATGAAAAATATCAACAATATCAGTTAGATGGAAATCTCCAGTACAAATGTGCCACATGTCGTGGAGAGTGCCACCAGGTCAAGAATCATGAGGATGCTGCGAAAGAGCTTTGGAAGCGAAAAGACGAAGCTGAACGAGATTTAATTACTAGCTTAAGGGCTGCTGCAGGGTTGCCAGCTCCTGAAGAAATATTTTCTATTCCTCCCTATTCTGATGATGAAGAAAATGGCCCTCCAATACAAAAGAGTGAATATGGACGTCCCTTGAAATTGTCTCTCAAAGGATTAGTTGATAAGTCGCCAAAAAAGACCAAGGACAGTGGAAAGAAATCTTTAATCAGAATCCCTGCTAAGAAGAAGGAATACCAGGAAATTTCAATCAGTAATATCGAAGCAAATCAGAGTGTTCGAGGACATGATCACCAATACCTTGGGTCGGACAAGAATGATGAGATGCATTCCTCTAAAAATGCAGAACCAGAAGTATATTCTTCACCTCTTGCTGGAAGTGTGAGCCATACTGAAGAGATTTTCTCTGTCAGTGAGCCAAGCGTATTGAAGCACAAGTTTGTTGATGAAGTGATGTTGAATGGTGGAGATAGGACCTCCAAAGTTCGTATTAAGGGCAAGTCTCATGGTCTGGATAGTGTAGAGGACACTGGGAAGCATGCTGGCAAGTCTAAGCCTGTGAAAGAGAAGAAGCTAGTGATAAACTTTGGTGCAAAAAAGTTAAATATAACTAAATCTCCAAGTTCTGATGCTTCAACCTATCAAAGAGAACATGCTTCGGTGACCTCCAATGGTGGTGAAGATGCGAGCCAGAGGACAACAAGTACTATGGTGGGAAATAGACATGATGGTTCTGCTAATTTTGGCGATGCTAAAGGTTCAAAGGTTGCTGGAAGAGAAGGAAATATTATAAAGTTGGGAAAGGCTAGGTCAGGAGCTCCAGATCATAACCCCAAACTTGTGAAAGGTGATAGGGTTGAGGAAGTCGAAGGCATTCCTCCGGAGCAGGCGCATGTTTCATCCGGAAAAAGCATTGAAGGAGGCATGACTGCAAGTGAGCCTGCAGTTGAGATCCCCACTATGAGAAATAACAAGGTATACTCAAGGAAGCACTCAGAAAGCAGGTCTGCTGTATCTGCTCATAGGAATGATGATTCTGGCCAAACACCAGTTTCACTTTCTTCCTCCAAAGATCATAAGCCTTTGTTGAAGTTTAAGCTTAAGAAACCTAATGTTGAAAGCCAGAGTTCTTCTCAGCATGAAGAAGAAAAAAGCTCTGTTAAGGGCCAACGGTCAAAAAGAAAGAGACCATCACCGTTTATGGAGAAAACATCATTTAGTGAAAATGATGATAGGGGACCGTCGGTTGAAGATAATTTAATGGATGAGATCATGGATGCTAATTGGATTTTGAAAAAGTTGGGAAAAGATGCAGTTGGAAAGCGAGTTGAAGTACAGCAGCTATCGGACAATTCATGGCACAAGGGAGTCGTTAGTGATGTTATTGAAGGCACATCACTGTTATCTGTTACTTTAGATGATGGCAAAGTCAAATCTCTGGAACTTGGAAAGCAAGGGGTTCGCTTTGTATCCCAAAAGCAGAAGAGGTCGAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1650

Amino Acids

181.71

Weight (kDa)

8.57

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 44 - 423 2.3e-36 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 476 - 613 2.1e-14 Plant cation/H(+) antiporter domain
CHX17_C PF23259 619 - 780 3e-15 Plant cation/H(+) antiporter, C-terminal domain
PHD PF00628 992 - 1035 1.7e-06 PHD-finger
PHDvar_NSD PF23004 992 - 1037 6.7e-06 Histone-lysine N-methyltransferase NSD-like, variant PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000666)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01660 AT5G01660 AT5G01660
fragaria_vesca FvH4_6g10590 FvH4_6g10591 FvH4_6g10591 FvH4_6g10591
malus_domestica MD04G1166900.v1.1 MD12G1180200.v1.1 MD12G1180500.v1.1
prunus_persica Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1
pyrus_communis pycom04g14830 pycom12g16990
rosa_chinensis RchiOBHm_Chr2g0145311 RchiOBHm_Chr3g0461571 RchiOBHm_Chr3g0461581
rosa_laevigata RLG00000024916 RLG00000024917
rosa_multiflora Rmu_sc0000790.1_g000001 Rmu_sc0003979.1_g000002 Rmu_sc0006964.1_g000002 Rmu_sc0010327.1_g000003 Rmu_ssc0000193.1_g000005 Rmu_ssc0000193.1_g000006
rosa_roxburghii Rroxscaffold_4G00330190 Rroxscaffold_6G00418120 Rroxscaffold_6G00418130 Rroxscaffold_6G00418490 Rroxscaffold_6G00418500
rosa_rugosa Rorug01G0017200 Rorug01G0017900 Rorug03G0051900 Rorug03G0052000 Rorug03G0052000 Rorug03G0052100
rosa_samantha Rh1CG027200 Rh1CG027400 Rh1CG027500 Rh1CG027700 Rh1CG073300 Rh1CG101500 Rh1CG101600 Rh1DG042300 Rh1DG042800 Rh3AG110200 Rh3AG110300 Rh3BG113400 Rh3BG113500 Rh3CG115300 Rh3CG115400 Rh3DG115100 Rh3DG115200 Rh5AG167800 Rh5BG556300 Rh5CG579000 Rh6AG136600 Rh7DG359300
rosa_wichuraiana Rw1G002200 Rw1G002230 Rw3G009320 Rw3G009330 Rw7G020340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4244
AarI CACCTGC 1 cut(s) 3188
AasI GACNNNNNNGTC 2 cut(s) 1526, 2372
AatII GACGTC 1 cut(s) 3586
Acc36I ACCTGC 3 cut(s) 159, 3188, 4446
AccB1I GGYRCC 2 cut(s) 1288, 2626
AccB7I CCANNNNNTGG 1 cut(s) 3146
AccI GTMKAC 4 cut(s) 1806, 1971, 1996, 4431
AccII CGCG 7 cut(s) 2429, 2508, 2552, 2618, 2663, 2676, 2702
AccIII TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
AclWI GGATC 8 cut(s) 308, 321, 645, 2409, 2480, 2993, 3326, 4399
AcoI YGGCCR 4 cut(s) 1735, 2601, 2712, 4492
AcsI RAATTY 7 cut(s) 21, 1369, 1797, 2291, 2431, 2970, 3696
AcuI CTGAAG 4 cut(s) 641, 1295, 3528, 3867
AcyI GRCGYC 3 cut(s) 2517, 2627, 3583
AdeI CACNNNGTG 1 cut(s) 1712
AflII CTTAAG 3 cut(s) 1957, 3478, 4553
AflIII ACRYGT 3 cut(s) 494, 2083, 3380
AhlI ACTAGT 2 cut(s) 202, 1480
AjnI CCWGG 7 cut(s) 387, 1420, 1936, 2529, 3139, 3399, 3690
AjuI GAANNNNNNNTTGG 2 cut(s) 2429, 2461
AleI CACNNNNGTG 1 cut(s) 3123
AloI GAACNNNNNNTCC 2 cut(s) 2983, 3015
Alw21I GWGCWC 4 cut(s) 337, 1242, 2026, 4273
Alw26I GTCTC 8 cut(s) 1075, 1276, 2074, 2132, 2922, 3603, 3960, 4633
Alw44I GTGCAC 1 cut(s) 1238
AlwI GGATC 8 cut(s) 308, 321, 645, 2409, 2480, 2993, 3326, 4399
AlwNI CAGNNNCTG 3 cut(s) 1421, 2288, 3506
Aor13HI TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
ApaLI GTGCAC 1 cut(s) 1238
ApoI RAATTY 7 cut(s) 21, 1369, 1797, 2291, 2431, 2970, 3696
ArsI GACNNNNNNTTYG 2 cut(s) 1799, 1831
Asp700I GAANNNNTTC 2 cut(s) 562, 4326
AspLEI GCGC 7 cut(s) 2554, 2629, 2665, 2676, 2678, 2691, 4345
AvaII GGWCC 7 cut(s) 463, 679, 1768, 2261, 2467, 3927, 4691
BaeGI GKGCMC 3 cut(s) 1242, 1291, 3101
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BalI TGGCCA 1 cut(s) 4494
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 2 cut(s) 1288, 2626
BanII GRGCYC 2 cut(s) 2506, 4273
BarI GAAGNNNNNNTAC 2 cut(s) 3671, 3703
BauI CACGAG 1 cut(s) 2962
Bbv12I GWGCWC 4 cut(s) 337, 1242, 2026, 4273
BbvCI CCTCAGC 2 cut(s) 1662, 2718
BceAI ACGGC 6 cut(s) 492, 1678, 2522, 2578, 2592, 2640
BcgI CGANNNNNNTGC 4 cut(s) 2943, 2977, 4895, 4929
BciT130I CCWGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
BciVI GTATCC 1 cut(s) 4933
BclI TGATCA 3 cut(s) 1849, 2316, 3742
BcoDI GTCTC 8 cut(s) 1075, 1276, 2074, 2132, 2922, 3603, 3960, 4633
BcuI ACTAGT 2 cut(s) 202, 1480
BfmI CTRYAG 4 cut(s) 1035, 1557, 3489, 4395
BfoI RGCGCY 1 cut(s) 2630
BfrI CTTAAG 3 cut(s) 1957, 3478, 4553
BfuAI ACCTGC 3 cut(s) 159, 3188, 4446
BfuI GTATCC 1 cut(s) 4933
BglII AGATCT 2 cut(s) 1841, 2929
BlpI GCTNAGC 1 cut(s) 2580
BmcAI AGTACT 2 cut(s) 2174, 4159
Bme1390I CCNGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
Bme18I GGWCC 7 cut(s) 463, 679, 1768, 2261, 2467, 3927, 4691
BmiI GGNNCC 5 cut(s) 315, 1290, 2262, 2628, 4692
BmrFI CCNGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
BmrI ACTGGG 2 cut(s) 733, 3993
BmuI ACTGGG 2 cut(s) 733, 3993
BplI GAGNNNNNCTC 4 cut(s) 3842, 3874, 4570, 4602
BpmI CTGGAG 7 cut(s) 1095, 2669, 2749, 2962, 3105, 3347, 4257
Bpu10I CCTNAGC 3 cut(s) 155, 1662, 2718
Bpu1102I GCTNAGC 1 cut(s) 2580
BpuEI CTTGAG 2 cut(s) 1975, 4420
Bsa29I ATCGAT 1 cut(s) 1860
BsaAI YACGTR 2 cut(s) 497, 1756
BsaBI GATNNNNATC 1 cut(s) 3357
BsaHI GRCGYC 3 cut(s) 2517, 2627, 3583
BsaI GGTCTC 4 cut(s) 1075, 2132, 2922, 4633
BsaWI WCCGGW 4 cut(s) 2251, 2492, 4333, 4355
BsaXI ACNNNNNCTCC 4 cut(s) 3078, 3108, 4054, 4084
Bse118I RCCGGY 2 cut(s) 1916, 2629
Bse3DI GCAATG 3 cut(s) 62, 546, 3301
Bse8I GATNNNNATC 1 cut(s) 3357
BseAI TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
BseBI CCWGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
BseCI ATCGAT 1 cut(s) 1860
BseJI GATNNNNATC 1 cut(s) 3357
BseMI GCAATG 3 cut(s) 62, 546, 3301
BseMII CTCAG 9 cut(s) 1653, 1786, 2127, 2322, 2494, 2594, 2709, 4461, 4601
BsePI GCGCGC 1 cut(s) 2674
BseRI GAGGAG 6 cut(s) 20, 578, 1592, 2553, 2622, 2784
BseSI GKGCMC 3 cut(s) 1242, 1291, 3101
BseYI CCCAGC 1 cut(s) 298
BsgI GTGCAG 1 cut(s) 1734
Bsh1236I CGCG 7 cut(s) 2429, 2508, 2552, 2618, 2663, 2676, 2702
Bsh1285I CGRYCG 2 cut(s) 2268, 2634
BshNI GGYRCC 2 cut(s) 1288, 2626
BshVI ATCGAT 1 cut(s) 1860
BsiEI CGRYCG 2 cut(s) 2268, 2634
BsiHKAI GWGCWC 4 cut(s) 337, 1242, 2026, 4273
BsiSI CCGG 8 cut(s) 1917, 2252, 2380, 2493, 2604, 2630, 4334, 4356
BslFI GGGAC 4 cut(s) 1423, 1735, 3570, 4704
BsmAI GTCTC 8 cut(s) 1075, 1276, 2074, 2132, 2922, 3603, 3960, 4633
BsmBI CGTCTC 1 cut(s) 1276
BsmFI GGGAC 4 cut(s) 1423, 1735, 3570, 4704
BsmI GAATGC 4 cut(s) 562, 564, 3781, 4326
Bso31I GGTCTC 4 cut(s) 1075, 2132, 2922, 4633
Bsp1286I GDGCHC 7 cut(s) 337, 1242, 1291, 2026, 2506, 3101, 4273
Bsp13I TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
Bsp1720I GCTNAGC 1 cut(s) 2580
Bsp19I CCATGG 1 cut(s) 459
BspCNI CTCAG 9 cut(s) 1654, 1785, 2128, 2321, 2493, 2593, 2710, 4460, 4600
BspDI ATCGAT 1 cut(s) 1860
BspEI TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
BspFNI CGCG 7 cut(s) 2429, 2508, 2552, 2618, 2663, 2676, 2702
BspHI TCATGA 3 cut(s) 717, 1846, 3412
BspLI GGNNCC 5 cut(s) 315, 1290, 2262, 2628, 4692
BspMAI CTGCAG 3 cut(s) 1561, 3493, 4399
BspMI ACCTGC 3 cut(s) 159, 3188, 4446
BspPI GGATC 8 cut(s) 308, 321, 645, 2409, 2480, 2993, 3326, 4399
BspQI GCTCTTC 2 cut(s) 380, 2778
BspT107I GGYRCC 2 cut(s) 1288, 2626
BspTI CTTAAG 3 cut(s) 1957, 3478, 4553
BspTNI GGTCTC 4 cut(s) 1075, 2132, 2922, 4633
BsrDI GCAATG 3 cut(s) 62, 546, 3301
BsrFI RCCGGY 2 cut(s) 1916, 2629
BssAI RCCGGY 2 cut(s) 1916, 2629
BssHII GCGCGC 1 cut(s) 2674
BssNAI GTATAC 1 cut(s) 4432
BssNI GRCGYC 3 cut(s) 2517, 2627, 3583
BssSI CACGAG 1 cut(s) 2962
BssT1I CCWWGG 6 cut(s) 459, 595, 872, 2436, 3636, 3754
Bst1107I GTATAC 1 cut(s) 4432
Bst2BI CACGAG 1 cut(s) 2962
Bst2UI CCWGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
Bst6I CTCTTC 9 cut(s) 380, 588, 877, 2028, 2188, 2778, 3843, 4222, 4931
BstACI GRCGYC 3 cut(s) 2517, 2627, 3583
BstAFI CTTAAG 3 cut(s) 1957, 3478, 4553
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 2 cut(s) 497, 1756
BstDSI CCRYGG 3 cut(s) 459, 2616, 2700
BstEII GGTNACC 1 cut(s) 4114
BstENI CCTNNNNNAGG 1 cut(s) 2453
BstFNI CGCG 7 cut(s) 2429, 2508, 2552, 2618, 2663, 2676, 2702
BstH2I RGCGCY 1 cut(s) 2630
BstHHI GCGC 7 cut(s) 2554, 2629, 2665, 2676, 2678, 2691, 4345
BstMAI GTCTC 8 cut(s) 1075, 1276, 2074, 2132, 2922, 3603, 3960, 4633
BstMCI CGRYCG 2 cut(s) 2268, 2634
BstNI CCWGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
BstNSI RCATGY 8 cut(s) 1639, 2087, 2958, 3195, 3384, 3995, 4109, 4349
BstPI GGTNACC 1 cut(s) 4114
BstSCI CCNGG 7 cut(s) 387, 1420, 1936, 2529, 3139, 3399, 3690
BstSFI CTRYAG 4 cut(s) 1035, 1557, 3489, 4395
BstSLI GKGCMC 3 cut(s) 1242, 1291, 3101
BstUI CGCG 7 cut(s) 2429, 2508, 2552, 2618, 2663, 2676, 2702
BstX2I RGATCY 6 cut(s) 313, 650, 1841, 2929, 2998, 4404
BstXI CCANNNNNNTGG 3 cut(s) 822, 3314, 3755
BstYI RGATCY 6 cut(s) 313, 650, 1841, 2929, 2998, 4404
BstZ17I GTATAC 1 cut(s) 4432
Bsu15I ATCGAT 1 cut(s) 1860
BsuI GTATCC 1 cut(s) 4933
BsuTUI ATCGAT 1 cut(s) 1860
BtgI CCRYGG 3 cut(s) 459, 2616, 2700
BtgZI GCGATG 1 cut(s) 4215
BtsI GCAGTG 1 cut(s) 1551
BveI ACCTGC 3 cut(s) 159, 3188, 4446
CaiI CAGNNNCTG 3 cut(s) 1421, 2288, 3506
CciI TCATGA 3 cut(s) 717, 1846, 3412
CfoI GCGC 7 cut(s) 2554, 2629, 2665, 2676, 2678, 2691, 4345
Cfr10I RCCGGY 2 cut(s) 1916, 2629
Cfr42I CCGCGG 2 cut(s) 2619, 2703
ClaI ATCGAT 1 cut(s) 1860
CseI GACGC 2 cut(s) 356, 2525
CsiI ACCWGGT 1 cut(s) 3399
CspCI CAANNNNNGTGG 4 cut(s) 1353, 1388, 3625, 3660
DinI GGCGCC 1 cut(s) 2628
DraIII CACNNNGTG 1 cut(s) 1712
DrdI GACNNNNNNGTC 2 cut(s) 1526, 2372
DseDI GACNNNNNNGTC 2 cut(s) 1526, 2372
EaeI YGGCCR 4 cut(s) 1735, 2601, 2712, 4492
Eam1104I CTCTTC 9 cut(s) 380, 588, 877, 2028, 2188, 2778, 3843, 4222, 4931
EarI CTCTTC 9 cut(s) 380, 588, 877, 2028, 2188, 2778, 3843, 4222, 4931
EciI GGCGGA 2 cut(s) 2556, 2580
Ecl136II GAGCTC 1 cut(s) 4271
Eco130I CCWWGG 6 cut(s) 459, 595, 872, 2436, 3636, 3754
Eco24I GRGCYC 2 cut(s) 2506, 4273
Eco31I GGTCTC 4 cut(s) 1075, 2132, 2922, 4633
Eco47I GGWCC 7 cut(s) 463, 679, 1768, 2261, 2467, 3927, 4691
Eco53kI GAGCTC 1 cut(s) 4271
Eco57I CTGAAG 4 cut(s) 641, 1295, 3528, 3867
Eco91I GGTNACC 1 cut(s) 4114
EcoICRI GAGCTC 1 cut(s) 4271
EcoNI CCTNNNNNAGG 1 cut(s) 2453
EcoO109I RGGNCCY 3 cut(s) 159, 1768, 3927
EcoO65I GGTNACC 1 cut(s) 4114
EcoRI GAATTC 2 cut(s) 1797, 2431
EcoRII CCWGG 7 cut(s) 387, 1420, 1936, 2529, 3139, 3399, 3690
EcoT14I CCWWGG 6 cut(s) 459, 595, 872, 2436, 3636, 3754
EcoT22I ATGCAT 4 cut(s) 564, 1655, 3193, 3783
EcoT38I GRGCYC 2 cut(s) 2506, 4273
EgeI GGCGCC 1 cut(s) 2628
EheI GGCGCC 1 cut(s) 2628
ErhI CCWWGG 6 cut(s) 459, 595, 872, 2436, 3636, 3754
Esp3I CGTCTC 1 cut(s) 1276
FalI AAGNNNNNCTT 4 cut(s) 425, 457, 2429, 2461
FaqI GGGAC 4 cut(s) 1423, 1735, 3570, 4704
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 3 cut(s) 1849, 2316, 3742
FblI GTMKAC 4 cut(s) 1806, 1971, 1996, 4431
FriOI GRGCYC 2 cut(s) 2506, 4273
GlaI GCGC 7 cut(s) 2553, 2628, 2664, 2675, 2677, 2690, 4344
GsaI CCCAGC 1 cut(s) 302
GsuI CTGGAG 7 cut(s) 1095, 2669, 2749, 2962, 3105, 3347, 4257
HaeII RGCGCY 1 cut(s) 2630
HapII CCGG 8 cut(s) 1917, 2252, 2380, 2493, 2604, 2630, 4334, 4356
HgaI GACGC 2 cut(s) 356, 2525
HhaI GCGC 7 cut(s) 2554, 2629, 2665, 2676, 2678, 2691, 4345
Hin1I GRCGYC 3 cut(s) 2517, 2627, 3583
Hin6I GCGC 7 cut(s) 2552, 2627, 2663, 2674, 2676, 2689, 4343
HinP1I GCGC 7 cut(s) 2552, 2627, 2663, 2674, 2676, 2689, 4343
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 3 cut(s) 969, 2045, 4550
HpaII CCGG 8 cut(s) 1917, 2252, 2380, 2493, 2604, 2630, 4334, 4356
Hpy99I CGWCG 6 cut(s) 2456, 2516, 2519, 2578, 2599, 4699
HpyCH4IV ACGT 4 cut(s) 496, 1755, 3135, 3583
HpySE526I ACGT 4 cut(s) 496, 1755, 3135, 3583
Hsp92I GRCGYC 3 cut(s) 2517, 2627, 3583
HspAI GCGC 7 cut(s) 2552, 2627, 2663, 2674, 2676, 2689, 4343
KasI GGCGCC 1 cut(s) 2626
Kpn2I TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
Ksp22I TGATCA 3 cut(s) 1849, 2316, 3742
KspI CCGCGG 2 cut(s) 2619, 2703
LguI GCTCTTC 2 cut(s) 380, 2778
LmnI GCTCC 7 cut(s) 1076, 1786, 1793, 3508, 4268, 4276, 4336
MabI ACCWGGT 1 cut(s) 3399
MaeII ACGT 4 cut(s) 496, 1755, 3135, 3583
MfeI CAATTG 1 cut(s) 2091
MflI RGATCY 6 cut(s) 313, 650, 1841, 2929, 2998, 4404
MhlI GDGCHC 7 cut(s) 337, 1242, 1291, 2026, 2506, 3101, 4273
MlsI TGGCCA 1 cut(s) 4494
MluNI TGGCCA 1 cut(s) 4494
Mly113I GGCGCC 1 cut(s) 2627
MlyI GAGTC 4 cut(s) 1661, 2651, 2750, 4830
MmeI TCCRAC 6 cut(s) 85, 2347, 2736, 2792, 3741, 4750
Mox20I TGGCCA 1 cut(s) 4494
Mph1103I ATGCAT 4 cut(s) 564, 1655, 3193, 3783
MroI TCCGGA 4 cut(s) 2251, 2492, 4333, 4355
MroXI GAANNNNTTC 2 cut(s) 562, 4326
MscI TGGCCA 1 cut(s) 4494
MslI CAYNNNNRTG 8 cut(s) 1208, 1938, 2953, 3072, 3123, 3312, 3417, 4479
Msp20I TGGCCA 1 cut(s) 4494
MspA1I CMGCKG 4 cut(s) 2618, 2702, 2717, 3123
MspCI CTTAAG 3 cut(s) 1957, 3478, 4553
MspI CCGG 8 cut(s) 1917, 2252, 2380, 2493, 2604, 2630, 4334, 4356
MspR9I CCNGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
MunI CAATTG 1 cut(s) 2091
Mva1269I GAATGC 4 cut(s) 562, 564, 3781, 4326
MvaI CCWGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
MvnI CGCG 7 cut(s) 2429, 2508, 2552, 2618, 2663, 2676, 2702
NarI GGCGCC 1 cut(s) 2627
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 319
NlaIV GGNNCC 5 cut(s) 315, 1290, 2262, 2628, 4692
NmuCI GTSAC 7 cut(s) 1305, 1513, 1528, 1549, 2620, 3116, 4114
NsiI ATGCAT 4 cut(s) 564, 1655, 3193, 3783
NspI RCATGY 8 cut(s) 1639, 2087, 2958, 3195, 3384, 3995, 4109, 4349
OliI CACNNNNGTG 1 cut(s) 3123
PaeI GCATGC 3 cut(s) 1639, 2958, 3995
PagI TCATGA 3 cut(s) 717, 1846, 3412
PaqCI CACCTGC 1 cut(s) 3188
PauI GCGCGC 1 cut(s) 2674
PciI ACATGT 2 cut(s) 2083, 3380
PciSI GCTCTTC 2 cut(s) 380, 2778
PcsI WCGNNNNNNNCGW 1 cut(s) 2523
PctI GAATGC 4 cut(s) 562, 564, 3781, 4326
PdmI GAANNNNTTC 2 cut(s) 562, 4326
PfeI GAWTC 9 cut(s) 861, 1103, 1425, 2385, 3251, 3257, 3409, 3666, 4487
PflFI GACNNNGTC 1 cut(s) 2456
PflMI CCANNNNNTGG 1 cut(s) 3146
Ple19I CGATCG 1 cut(s) 2268
PleI GAGTC 4 cut(s) 1661, 2650, 2749, 4829
PluTI GGCGCC 1 cut(s) 2630
PpsI GAGTC 4 cut(s) 1661, 2650, 2749, 4829
Ppu21I YACGTR 2 cut(s) 497, 1756
PpuMI RGGWCCY 2 cut(s) 1768, 3927
PscI ACATGT 2 cut(s) 2083, 3380
PsiI TTATAA 1 cut(s) 4244
Psp124BI GAGCTC 1 cut(s) 4273
Psp5II RGGWCCY 2 cut(s) 1768, 3927
Psp6I CCWGG 7 cut(s) 387, 1420, 1936, 2529, 3139, 3399, 3690
PspEI GGTNACC 1 cut(s) 4114
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 7 cut(s) 387, 1420, 1936, 2529, 3139, 3399, 3690
PspN4I GGNNCC 5 cut(s) 315, 1290, 2262, 2628, 4692
PspPPI RGGWCCY 2 cut(s) 1768, 3927
PstI CTGCAG 3 cut(s) 1561, 3493, 4399
PstNI CAGNNNCTG 3 cut(s) 1421, 2288, 3506
PsuI RGATCY 6 cut(s) 313, 650, 1841, 2929, 2998, 4404
PsyI GACNNNGTC 1 cut(s) 2456
PteI GCGCGC 1 cut(s) 2674
PvuI CGATCG 1 cut(s) 2268
PvuII CAGCTG 1 cut(s) 3123
RseI CAYNNNNRTG 8 cut(s) 1208, 1938, 2953, 3072, 3123, 3312, 3417, 4479
SacI GAGCTC 1 cut(s) 4273
SacII CCGCGG 2 cut(s) 2619, 2703
SapI GCTCTTC 2 cut(s) 380, 2778
ScaI AGTACT 2 cut(s) 2174, 4159
SchI GAGTC 4 cut(s) 1661, 2651, 2750, 4830
ScrFI CCNGG 7 cut(s) 389, 1422, 1938, 2531, 3141, 3401, 3692
SduI GDGCHC 7 cut(s) 337, 1242, 1291, 2026, 2506, 3101, 4273
SexAI ACCWGGT 1 cut(s) 3399
SfcI CTRYAG 4 cut(s) 1035, 1557, 3489, 4395
SfoI GGCGCC 1 cut(s) 2628
Sfr303I CCGCGG 2 cut(s) 2619, 2703
SgrBI CCGCGG 2 cut(s) 2619, 2703
SinI GGWCC 7 cut(s) 463, 679, 1768, 2261, 2467, 3927, 4691
SmiMI CAYNNNNRTG 8 cut(s) 1208, 1938, 2953, 3072, 3123, 3312, 3417, 4479
SmlI CTYRAG 5 cut(s) 1957, 1990, 3478, 4435, 4553
SmoI CTYRAG 5 cut(s) 1957, 1990, 3478, 4435, 4553
SpeI ACTAGT 2 cut(s) 202, 1480
SphI GCATGC 3 cut(s) 1639, 2958, 3995
SspDI GGCGCC 1 cut(s) 2626
SspI AATATT 3 cut(s) 235, 3516, 4240
SstI GAGCTC 1 cut(s) 4273
StyD4I CCNGG 7 cut(s) 387, 1420, 1936, 2529, 3139, 3399, 3690
StyI CCWWGG 6 cut(s) 459, 595, 872, 2436, 3636, 3754
TaiI ACGT 4 cut(s) 499, 1758, 3138, 3586
TatI WGTACW 6 cut(s) 258, 487, 2172, 3366, 4157, 4786
TfiI GAWTC 9 cut(s) 861, 1103, 1425, 2385, 3251, 3257, 3409, 3666, 4487
TseFI GTSAC 7 cut(s) 1305, 1513, 1528, 1549, 2620, 3116, 4114
Tsp45I GTSAC 7 cut(s) 1305, 1513, 1528, 1549, 2620, 3116, 4114
TspGWI ACGGA 2 cut(s) 1780, 2478
Tth111I GACNNNGTC 1 cut(s) 2456
Van91I CCANNNNNTGG 1 cut(s) 3146
Vha464I CTTAAG 3 cut(s) 1957, 3478, 4553
VneI GTGCAC 1 cut(s) 1238
VpaK11BI GGWCC 7 cut(s) 463, 679, 1768, 2261, 2467, 3927, 4691
XagI CCTNNNNNAGG 1 cut(s) 2453
XapI RAATTY 7 cut(s) 21, 1369, 1797, 2291, 2431, 2970, 3696
XceI RCATGY 8 cut(s) 1639, 2087, 2958, 3195, 3384, 3995, 4109, 4349
XcmI CCANNNNNNNNNTGG 3 cut(s) 1207, 2330, 2773
XmiI GTMKAC 4 cut(s) 1806, 1971, 1996, 4431
XmnI GAANNNNTTC 2 cut(s) 562, 4326
ZraI GACGTC 1 cut(s) 3584
ZrmI AGTACT 2 cut(s) 2174, 4159
Zsp2I ATGCAT 4 cut(s) 564, 1655, 3193, 3783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.