pycom12g16990

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000666
Physical Location & Seq
Reverse (-)
19272025 .. 19273207
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16990.3

Sequence Viewer

Length: 333 bp
ATGCATTCGAAAAGAAGCTGCGATTCATCGGTTGTTCTTAATGAAAAAATATATGTTCTGGGAGGCTTTGATGGAGATTCACTGGTCCAGAGCGTGGAAAGTTTTGATCCACGGCTTGGGTCATGGATGCCGGCTGAACCAATGTATTTCCCCAGGGGATATCCAGCAGCTGCTATTGTCGAAGATACCATGTATGTAATCGGAGGGATGAAAGACGATGAAAACATAGCAGAGGCAGTCGAATACTACTACGAGGGGCAAGATTGGCGGGAAATGGCCACGGTGACCATCGGTAAAAGGTGTTTCATGTCCGCGGCTGCTTTTTCGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

12.29

Weight (kDa)

4.43

Isoelectric Point (pI)

69.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000666)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01660 AT5G01660 AT5G01660
fragaria_vesca FvH4_6g10590 FvH4_6g10591 FvH4_6g10591 FvH4_6g10591
malus_domestica MD04G1166900.v1.1 MD12G1180200.v1.1 MD12G1180500.v1.1
prunus_persica Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1
pyrus_communis pycom04g14830 pycom12g16990
rosa_chinensis RchiOBHm_Chr2g0145311 RchiOBHm_Chr3g0461571 RchiOBHm_Chr3g0461581
rosa_laevigata RLG00000024916 RLG00000024917
rosa_multiflora Rmu_sc0000790.1_g000001 Rmu_sc0003979.1_g000002 Rmu_sc0006964.1_g000002 Rmu_sc0010327.1_g000003 Rmu_ssc0000193.1_g000005 Rmu_ssc0000193.1_g000006
rosa_roxburghii Rroxscaffold_4G00330190 Rroxscaffold_6G00418120 Rroxscaffold_6G00418130 Rroxscaffold_6G00418490 Rroxscaffold_6G00418500
rosa_rugosa Rorug01G0017200 Rorug01G0017900 Rorug03G0051900 Rorug03G0052000 Rorug03G0052000 Rorug03G0052100
rosa_samantha Rh1CG027200 Rh1CG027400 Rh1CG027500 Rh1CG027700 Rh1CG073300 Rh1CG101500 Rh1CG101600 Rh1DG042300 Rh1DG042800 Rh3AG110200 Rh3AG110300 Rh3BG113400 Rh3BG113500 Rh3CG115300 Rh3CG115400 Rh3DG115100 Rh3DG115200 Rh5AG167800 Rh5BG556300 Rh5CG579000 Rh6AG136600 Rh7DG359300
rosa_wichuraiana Rw1G002200 Rw1G002230 Rw3G009320 Rw3G009330 Rw7G020340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 94, 116
AccII CGCG 1 cut(s) 314
AciI CCGC 3 cut(s) 268, 312, 314
AclWI GGATC 1 cut(s) 101
AcoI YGGCCR 1 cut(s) 276
AfiI CCNNNNNNNGG 2 cut(s) 94, 116
AjnI CCWGG 1 cut(s) 152
AluBI AGCT 2 cut(s) 18, 170
AluI AGCT 2 cut(s) 18, 170
AlwI GGATC 1 cut(s) 101
AlwNI CAGNNNCTG 1 cut(s) 170
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 4 cut(s) 18, 167, 170, 317
AspS9I GGNCC 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 1 cut(s) 85
BalI TGGCCA 1 cut(s) 278
BbvI GCAGC 4 cut(s) 5, 157, 179, 304
BccI CCATC 2 cut(s) 65, 296
BceAI ACGGC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 154
BisI GCNGC 5 cut(s) 19, 168, 171, 315, 318
BlsI GCNGC 5 cut(s) 20, 169, 172, 316, 319
Bme1390I CCNGG 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 154
BmsI GCATC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 8
BsaJI CCNNGG 5 cut(s) 110, 152, 153, 279, 312
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 116
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 5 cut(s) 110, 152, 153, 279, 312
BseGI GGATG 2 cut(s) 132, 213
BseLI CCNNNNNNNGG 2 cut(s) 94, 116
BseNI ACTGG 1 cut(s) 87
BseXI GCAGC 4 cut(s) 5, 157, 179, 304
Bsh1236I CGCG 1 cut(s) 314
BshFI GGCC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 94, 116
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 1 cut(s) 278
Bsp119I TTCGAA 1 cut(s) 8
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 3 cut(s) 268, 312, 314
BspANI GGCC 1 cut(s) 278
BspFNI CGCG 1 cut(s) 314
BspPI GGATC 1 cut(s) 101
BspT104I TTCGAA 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 5 cut(s) 110, 152, 153, 279, 312
BssMI GATC 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 132
BstDSI CCRYGG 3 cut(s) 110, 279, 312
BstEII GGTNACC 1 cut(s) 283
BstF5I GGATG 2 cut(s) 132, 213
BstFNI CGCG 1 cut(s) 314
BstKTI GATC 1 cut(s) 109
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstNI CCWGG 1 cut(s) 154
BstPI GGTNACC 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 152
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 4 cut(s) 5, 157, 179, 304
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 3 cut(s) 110, 279, 312
BtsCI GGATG 2 cut(s) 132, 213
BtsIMutI CAGTG 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 132
CaiI CAGNNNCTG 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 85
Cfr42I CCGCGG 1 cut(s) 315
CviAII CATG 3 cut(s) 123, 190, 307
CviJI RGCY 7 cut(s) 18, 66, 115, 134, 170, 278, 317
CviKI_1 RGCY 7 cut(s) 18, 66, 115, 134, 170, 278, 317
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 276
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 85
Eco91I GGTNACC 1 cut(s) 283
EcoO65I GGTNACC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 152
EcoRV GATATC 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 126, 193, 310
FaiI YATR 7 cut(s) 52, 54, 124, 191, 195, 227, 308
FatI CATG 3 cut(s) 122, 189, 306
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 19, 168, 171, 315, 318
FokI GGATG 2 cut(s) 139, 220
Fsp4HI GCNGC 5 cut(s) 19, 168, 171, 315, 318
GluI GCNGC 5 cut(s) 19, 168, 171, 315, 318
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 131
Hin1II CATG 3 cut(s) 126, 193, 310
HinfI GANTC 2 cut(s) 23, 77
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 295
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
Hsp92II CATG 3 cut(s) 126, 193, 310
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
KspI CCGCGG 1 cut(s) 315
Kzo9I GATC 1 cut(s) 106
LpnPI CCDG 7 cut(s) 44, 68, 101, 139, 144, 166, 177
Lsp1109I GCAGC 4 cut(s) 5, 157, 179, 304
LweI GCATC 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 283
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 1 cut(s) 194
MlsI TGGCCA 1 cut(s) 278
MluNI TGGCCA 1 cut(s) 278
MnlI CCTC 4 cut(s) 56, 197, 226, 247
Mox20I TGGCCA 1 cut(s) 278
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 130
MscI TGGCCA 1 cut(s) 278
MseI TTAA 1 cut(s) 39
Msp20I TGGCCA 1 cut(s) 278
MspA1I CMGCKG 2 cut(s) 170, 314
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 154
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 1 cut(s) 154
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 265
NaeI GCCGGC 1 cut(s) 132
NdeII GATC 1 cut(s) 106
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 3 cut(s) 126, 193, 310
NmuCI GTSAC 1 cut(s) 283
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 8
PasI CCCWGGG 1 cut(s) 153
PctI GAATGC 1 cut(s) 4
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 2 cut(s) 23, 77
PflMI CCANNNNNTGG 2 cut(s) 94, 116
PkrI GCNGC 5 cut(s) 20, 169, 172, 316, 319
Psp6I CCWGG 1 cut(s) 152
PspEI GGTNACC 1 cut(s) 283
PspGI CCWGG 1 cut(s) 152
PspPI GGNCC 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 170
SacII CCGCGG 1 cut(s) 315
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 5 cut(s) 19, 168, 171, 315, 318
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 154
SetI ASST 4 cut(s) 20, 172, 302, 332
SfaNI GCATC 1 cut(s) 117
Sfr303I CCGCGG 1 cut(s) 315
SfuI TTCGAA 1 cut(s) 8
SgrBI CCGCGG 1 cut(s) 315
SinI GGWCC 1 cut(s) 85
SsiI CCGC 3 cut(s) 268, 312, 314
StyD4I CCNGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 283
TaqI TCGA 4 cut(s) 8, 180, 240, 326
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 2 cut(s) 23, 77
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 4 cut(s) 18, 167, 170, 317
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 5 cut(s) 15, 57, 224, 234, 295
TspRI CASTG 1 cut(s) 87
Van91I CCANNNNNTGG 2 cut(s) 94, 116
VpaK11BI GGWCC 1 cut(s) 85
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.