Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0000666

Physical Location & Seq

Genomic Coordinates Forward (+)

8939303 .. 8945191

Gene Structure 5889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G009330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGAATATTGATCCATATGGTTGGACAACTGATGGATCAGTGAGAACACAATTTCCTGCACAGGTTCAGATTCGTGTCCAGCTGCAGTGCCAACCACTGCTTGAGAGTCAGTTTAAACTGATTATTGCGGACAACTACTACAGTGCAAATAAATTCTGGTTTGAGCTTGATCATGCTCAGACAGACAAGCTGATGTCTTTGTTAGCATCTTGCCCAATCGCTCCAGGTACTAAAGTACCGCAGAAAATACCAAAGAGGAAAACTGATCTTCCAATGCAACCCTCACGTAAGACAACAGTGGAAGCTCAGTGGCTTAAACCACTCACTTCAGGGGCGAAAACACTAGTTGGAAACTACAATCCATCTGAAGCTGTATTAGATGTGAAAGACGCTGAACAAGAAGAACAGCACCTCATTTGCGCAAAACTGAAAGAGTTGTCTCTTCAACGTGATCTCAACAGTGAATACCAAAATGTGCCTTTGTCAATAGATGTTAAAGATAGTGCTATTGTTAATGCAACGCCTGTAGCGGTCAATTGGAATCCACCTGAACCATTGGGTTTAGTGGAGATGAGGGTAGAGAGGTCTCATTCCTGGTCTGAGAAGAAGAGTGGAGGAAGTCCTTGCTCATTGTCTGCGTTGAGTCCTCGATCCTCGTCTGATCAGTCACAAATCGATAAGTTGGTTCAAGAGGTTGAAGAGTTAAAAGCTTTCAAGAATGAACAAGCTAAGAAGATAGGCTATTTGGAGCATAAGCTGGAGCAGGCACAGTTTGAAATTAAACAGTTAAAAGATGGTATAAAGTCGGAATCTGAGTCTACACCATCCCTTGCACATATTGATGAAAAGCAGTCAGAGTCTGAGATTCAGTTAAAAGACGGTATGAAGTTGGAATGTGAGTCTGTACCCTCCACACAAGAGAAGGATATTGCATCGTGCGATATGCTGGATCTGGATCCTAATGAGTCAATATATCTAGTTGGAGGATCTGATGGTGAATCATGGTTGTCATTGGATTCTTATTATCCTTCTCGAAATGTGATAAAATCTCTTGGACCAATGAGCTCAGATCGTGGATATGCTTCTGTTGCAAAATTAAATGGTGATCTCTATGTGATTGGAGGTGGTGCTAGTGCTGTAGGTGGTGATGGTGCTGTGTGGTATGATACAGTTGAATCATACTGCCCTGCTGACGAGCAGTGGAAGGTCTGCCCTTCTTTGAGAGAGAAAAAGGGGAGCTTAGCTGCAGCTACCACAAACAATAAAATTTTTGCAATGGGTGGCGGCAATGGAATTGAGTGTTTTTCTGATGTTGAAATGCTTGATTTAGATGTTGGTCGATGGATCCGTACACAGTCAATGCTACAAAAGAGATTTGCTCTCGCTGCGGCAGAACTAAATGGAGTAATATACGCTACTGGAGGGTATGATGGGAATGGTTATTTGAAGTCCGTTGATAGATTTGATCCAAGGGAGCATAGTTGGAAGAAAGTCCCTTGTATGAAATCAGAAAGGGGTTGCCATTCATTGGTTACATTAAATGAAAAATTGTATGCTCTGGGTGGTCATGATGGAGATTCTATGGTGTCAAGTGTAGAAATTTTTGACCCACGCCTTGAGTCATGGATGCCTGGGGAACCAATGAACTACTCTAGGGGATATTCGGCTGCGGCTGTTGTTAATGATACCATCTATGTAATTGGAGGGGTGAAAGGGGATACCAGCATTGCAGAGGCTGTTGAATGCTACAAGGAGGGCCAAGGTTGGCAAGAAATGGCGGGGGTGATTAGCAAAAGGTGCTTCCTGTCGGCCACTGCTTACTCGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005856 GO:0008150 GO:0008582 GO:0040008 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044087 GO:0044424 GO:0044464 GO:0045886 GO:0045926 GO:0048519 GO:0048523 GO:0048638 GO:0048640 GO:0050789 GO:0050793 GO:0050794 GO:0050803 GO:0050807 GO:0051093 GO:0051128 GO:0051129 GO:0051239 GO:0051241 GO:0051960 GO:0051961 GO:0051963 GO:0051964 GO:0065007 GO:0065008 GO:1904396 GO:1904397 GO:1905809 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Dev_Cell_Death Kelch_1 Kelch_5

Protein Analysis

609

Amino Acids

67.05

Weight (kDa)

5.04

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dev_Cell_Death	PF10539	1 - 67	3.8e-19	Development and cell death domain
Beta-prop_FBX42	PF13415	320 - 493	3.8e-08	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	321 - 453	3.1e-07	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1	PF01344	358 - 407	1.3e-06	Kelch motif
Kelch_FKB95	PF25210	406 - 506	6.4e-10	FKB95, Kelch-repeats domain
Kelch_1	PF01344	415 - 454	2.7e-07	Kelch motif
Beta-prop_Calicin	PF13964	446 - 585	1.5e-15	Calicin, beta-propeller domain
Kelch_1	PF01344	460 - 502	6e-13	Kelch motif
Kelch_KLHDC2_KLHL20_DRC7	PF24681	463 - 569	2.5e-12	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	485 - 580	1.5e-06	FBX42, beta-propeller domain
Kelch_FKB95	PF25210	498 - 583	2.9e-11	FKB95, Kelch-repeats domain
Kelch_1	PF01344	505 - 549	8.9e-11	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01660 AT5G01660 AT5G01660
fragaria_vesca	FvH4_6g10590 FvH4_6g10591 FvH4_6g10591 FvH4_6g10591
malus_domestica	MD04G1166900.v1.1 MD12G1180200.v1.1 MD12G1180500.v1.1
prunus_persica	Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285100_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1 Prupe.6G285200_v2.0.a1
pyrus_communis	pycom04g14830 pycom12g16990
rosa_chinensis	RchiOBHm_Chr2g0145311 RchiOBHm_Chr3g0461571 RchiOBHm_Chr3g0461581
rosa_laevigata	RLG00000024916 RLG00000024917
rosa_multiflora	Rmu_sc0000790.1_g000001 Rmu_sc0003979.1_g000002 Rmu_sc0006964.1_g000002 Rmu_sc0010327.1_g000003 Rmu_ssc0000193.1_g000005 Rmu_ssc0000193.1_g000006
rosa_roxburghii	Rroxscaffold_4G00330190 Rroxscaffold_6G00418120 Rroxscaffold_6G00418130 Rroxscaffold_6G00418490 Rroxscaffold_6G00418500
rosa_rugosa	Rorug01G0017200 Rorug01G0017900 Rorug03G0051900 Rorug03G0052000 Rorug03G0052000 Rorug03G0052100
rosa_samantha	Rh1CG027200 Rh1CG027400 Rh1CG027500 Rh1CG027700 Rh1CG073300 Rh1CG101500 Rh1CG101600 Rh1DG042300 Rh1DG042800 Rh3AG110200 Rh3AG110300 Rh3BG113400 Rh3BG113500 Rh3CG115300 Rh3CG115400 Rh3DG115100 Rh3DG115200 Rh5AG167800 Rh5BG556300 Rh5CG579000 Rh6AG136600 Rh7DG359300
rosa_wichuraiana	Rw1G002200 Rw1G002230 Rw3G009320 Rw3G009330 Rw7G020340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	421
AccB7I	CCANNNNNTGG	1 cut(s)	1528
AccI	GTMKAC	1 cut(s)	818
AciI	CCGC	7 cut(s)	128, 239, 530, 1284, 1388, 1670, 1778
AcoI	YGGCCR	1 cut(s)	1809
AcsI	RAATTY	3 cut(s)	152, 1266, 1599
AcuI	CTGAAG	2 cut(s)	312, 387
AfaI	GTAC	4 cut(s)	229, 237, 906, 1351
AfiI	CCNNNNNNNGG	1 cut(s)	1528
AgsI	TTSAA	9 cut(s)	446, 689, 698, 715, 776, 1175, 1316, 1447, 1742
AhlI	ACTAGT	1 cut(s)	343
AjnI	CCWGG	3 cut(s)	223, 593, 1630
AloI	GAACNNNNNNTCC	2 cut(s)	37, 69
Alw21I	GWGCWC	1 cut(s)	1067
Alw26I	GTCTC	2 cut(s)	444, 591
AlwNI	CAGNNNCTG	2 cut(s)	860, 1736
AoxI	GGCC	2 cut(s)	1756, 1809
ApeKI	GCWGC	5 cut(s)	82, 1244, 1247, 1385, 1667
ApoI	RAATTY	3 cut(s)	152, 1266, 1599
AspLEI	GCGC	1 cut(s)	422
AspS9I	GGNCC	2 cut(s)	1055, 1756
AsuHPI	GGTGA	5 cut(s)	1007, 1115, 1157, 1720, 1795
AvaII	GGWCC	1 cut(s)	1055
BaeI	ACNNNNGTAYC	4 cut(s)	219, 219, 252, 252
BamHI	GGATCC	2 cut(s)	955, 1344
BanII	GRGCYC	1 cut(s)	1067
Bbv12I	GWGCWC	1 cut(s)	1067
BbvI	GCAGC	5 cut(s)	69, 1231, 1259, 1372, 1654
BciT130I	CCWGG	3 cut(s)	225, 595, 1632
BciVI	GTATCC	1 cut(s)	1711
BclI	TGATCA	2 cut(s)	169, 661
BcoDI	GTCTC	2 cut(s)	444, 591
BcuI	ACTAGT	1 cut(s)	343
BfaI	CTAG	4 cut(s)	344, 977, 1131, 1653
BfmI	CTRYAG	5 cut(s)	83, 139, 525, 1137, 1245
BfuI	GTATCC	1 cut(s)	1711
BisI	GCNGC	8 cut(s)	83, 1245, 1248, 1285, 1386, 1389, 1668, 1671
BlpI	GCTNAGC	1 cut(s)	1240
BlsI	GCNGC	8 cut(s)	84, 1246, 1249, 1286, 1387, 1390, 1669, 1672
Bme1390I	CCNGG	3 cut(s)	225, 595, 1632
Bme18I	GGWCC	1 cut(s)	1055
BmgT120I	GGNCC	2 cut(s)	1055, 1756
BmiI	GGNNCC	3 cut(s)	957, 1346, 1638
BmrFI	CCNGG	3 cut(s)	225, 595, 1632
BmsI	GCATC	3 cut(s)	215, 941, 1617
BplI	GAGNNNNNCTC	2 cut(s)	1363, 1395
BpmI	CTGGAG	3 cut(s)	207, 779, 1440
Bpu1102I	GCTNAGC	1 cut(s)	1240
BpuEI	CTTGAG	2 cut(s)	122, 1637
Bsa29I	ATCGAT	1 cut(s)	675
BsaAI	YACGTR	1 cut(s)	287
BsaBI	GATNNNNATC	1 cut(s)	954
BsaI	GGTCTC	1 cut(s)	591
BsaJI	CCNNGG	3 cut(s)	1469, 1631, 1759
Bsc4I	CCNNNNNNNGG	1 cut(s)	1528
Bse1I	ACTGG	1 cut(s)	1423
Bse3DI	GCAATG	3 cut(s)	1281, 1294, 1725
Bse8I	GATNNNNATC	1 cut(s)	954
BseBI	CCWGG	3 cut(s)	225, 595, 1632
BseCI	ATCGAT	1 cut(s)	675
BseDI	CCNNGG	3 cut(s)	1469, 1631, 1759
BseGI	GGATG	2 cut(s)	824, 1632
BseJI	GATNNNNATC	1 cut(s)	954
BseLI	CCNNNNNNNGG	1 cut(s)	1528
BseMI	GCAATG	3 cut(s)	1281, 1294, 1725
BseMII	CTCAG	6 cut(s)	191, 320, 591, 804, 852, 1080
BseNI	ACTGG	1 cut(s)	1423
BseXI	GCAGC	5 cut(s)	69, 1231, 1259, 1372, 1654
BsgI	GTGCAG	1 cut(s)	42
BshFI	GGCC	2 cut(s)	1758, 1811
BshVI	ATCGAT	1 cut(s)	675
BsiHKAI	GWGCWC	1 cut(s)	1067
BslFI	GGGAC	1 cut(s)	1478
BslI	CCNNNNNNNGG	1 cut(s)	1528
BsmAI	GTCTC	2 cut(s)	444, 591
BsmFI	GGGAC	1 cut(s)	1478
BsmI	GAATGC	1 cut(s)	1748
BsnI	GGCC	2 cut(s)	1758, 1811
Bso31I	GGTCTC	1 cut(s)	591
Bsp1286I	GDGCHC	1 cut(s)	1067
Bsp1720I	GCTNAGC	1 cut(s)	1240
BspACI	CCGC	7 cut(s)	128, 239, 530, 1284, 1388, 1670, 1778
BspANI	GGCC	2 cut(s)	1758, 1811
BspCNI	CTCAG	6 cut(s)	190, 319, 592, 805, 853, 1079
BspDI	ATCGAT	1 cut(s)	675
BspHI	TCATGA	1 cut(s)	1567
BspLI	GGNNCC	3 cut(s)	957, 1346, 1638
BspMAI	CTGCAG	2 cut(s)	87, 1249
BspTNI	GGTCTC	1 cut(s)	591
BsrDI	GCAATG	3 cut(s)	1281, 1294, 1725
BsrI	ACTGG	1 cut(s)	1423
BssECI	CCNNGG	3 cut(s)	1469, 1631, 1759
BssT1I	CCWWGG	2 cut(s)	1469, 1759
Bst2UI	CCWGG	3 cut(s)	225, 595, 1632
Bst4CI	ACNGT	8 cut(s)	143, 298, 461, 771, 786, 881, 1171, 1356
Bst6I	CTCTTC	3 cut(s)	447, 602, 693
BstAPI	GCANNNNNTGC	1 cut(s)	1797
BstBAI	YACGTR	1 cut(s)	287
BstC8I	GCNNGC	1 cut(s)	765
BstDEI	CTNAG	8 cut(s)	177, 306, 600, 729, 813, 861, 1066, 1240
BstF5I	GGATG	2 cut(s)	824, 1632
BstHHI	GCGC	1 cut(s)	422
BstMAI	GTCTC	2 cut(s)	444, 591
BstMWI	GCNNNNNNNGC	3 cut(s)	1088, 1385, 1797
BstNI	CCWGG	3 cut(s)	225, 595, 1632
BstSCI	CCNGG	3 cut(s)	223, 593, 1630
BstSFI	CTRYAG	5 cut(s)	83, 139, 525, 1137, 1245
BstV1I	GCAGC	5 cut(s)	69, 1231, 1259, 1372, 1654
BstX2I	RGATCY	4 cut(s)	949, 955, 986, 1344
BstXI	CCANNNNNNTGG	1 cut(s)	21
BstYI	RGATCY	4 cut(s)	949, 955, 986, 1344
Bsu15I	ATCGAT	1 cut(s)	675
BsuI	GTATCC	1 cut(s)	1711
BsuRI	GGCC	2 cut(s)	1758, 1811
BsuTUI	ATCGAT	1 cut(s)	675
BtsCI	GGATG	2 cut(s)	824, 1632
BtsI	GCAGTG	4 cut(s)	92, 95, 1205, 1812
BtsIMutI	CAGTG	9 cut(s)	45, 92, 95, 148, 303, 314, 466, 1205, 1812
Cac8I	GCNNGC	1 cut(s)	765
CaiI	CAGNNNCTG	2 cut(s)	860, 1736
CciI	TCATGA	1 cut(s)	1567
CfoI	GCGC	1 cut(s)	422
Cfr13I	GGNCC	2 cut(s)	1055, 1756
ClaI	ATCGAT	1 cut(s)	675
CseI	GACGC	1 cut(s)	398
Csp6I	GTAC	4 cut(s)	228, 236, 905, 1350
CviAII	CATG	4 cut(s)	173, 1002, 1568, 1623
CviQI	GTAC	4 cut(s)	228, 236, 905, 1350
DdeI	CTNAG	8 cut(s)	177, 306, 600, 729, 813, 861, 1066, 1240
DraI	TTTAAA	1 cut(s)	115
EaeI	YGGCCR	1 cut(s)	1809
Eam1104I	CTCTTC	3 cut(s)	447, 602, 693
EarI	CTCTTC	3 cut(s)	447, 602, 693
Ecl136II	GAGCTC	1 cut(s)	1065
Eco130I	CCWWGG	2 cut(s)	1469, 1759
Eco24I	GRGCYC	1 cut(s)	1067
Eco31I	GGTCTC	1 cut(s)	591
Eco47I	GGWCC	1 cut(s)	1055
Eco53kI	GAGCTC	1 cut(s)	1065
Eco57I	CTGAAG	2 cut(s)	312, 387
EcoICRI	GAGCTC	1 cut(s)	1065
EcoRII	CCWGG	3 cut(s)	223, 593, 1630
EcoT14I	CCWWGG	2 cut(s)	1469, 1759
EcoT38I	GRGCYC	1 cut(s)	1067
ErhI	CCWWGG	2 cut(s)	1469, 1759
FaeI	CATG	4 cut(s)	176, 1005, 1571, 1626
FalI	AAGNNNNNCTT	2 cut(s)	1223, 1255
FaqI	GGGAC	1 cut(s)	1478
FatI	CATG	4 cut(s)	172, 1001, 1567, 1622
FauI	CCCGC	1 cut(s)	1771
FauNDI	CATATG	1 cut(s)	16
FbaI	TGATCA	2 cut(s)	169, 661
FblI	GTMKAC	1 cut(s)	818
Fnu4HI	GCNGC	8 cut(s)	83, 1245, 1248, 1285, 1386, 1389, 1668, 1671
FokI	GGATG	2 cut(s)	811, 1639
FriOI	GRGCYC	1 cut(s)	1067
Fsp4HI	GCNGC	8 cut(s)	83, 1245, 1248, 1285, 1386, 1389, 1668, 1671
FspBI	CTAG	4 cut(s)	344, 977, 1131, 1653
FspI	TGCGCA	1 cut(s)	421
GlaI	GCGC	1 cut(s)	421
GluI	GCNGC	8 cut(s)	83, 1245, 1248, 1285, 1386, 1389, 1668, 1671
GsuI	CTGGAG	3 cut(s)	207, 779, 1440
HaeIII	GGCC	2 cut(s)	1758, 1811
HgaI	GACGC	1 cut(s)	398
HhaI	GCGC	1 cut(s)	422
Hin1II	CATG	4 cut(s)	176, 1005, 1571, 1626
Hin6I	GCGC	1 cut(s)	420
HinP1I	GCGC	1 cut(s)	420
HindIII	AAGCTT	1 cut(s)	708
HphI	GGTGA	5 cut(s)	1007, 1115, 1157, 1720, 1795
Hpy166II	GTNNAC	2 cut(s)	819, 1352
Hpy188III	TCNNGA	5 cut(s)	689, 715, 953, 1032, 1568
Hpy8I	GTNNAC	2 cut(s)	819, 1352
HpyAV	CCTTC	4 cut(s)	916, 1038, 1198, 1224
HpyCH4III	ACNGT	8 cut(s)	143, 298, 461, 771, 786, 881, 1171, 1356
HpyCH4IV	ACGT	2 cut(s)	286, 448
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1088, 1385, 1797
HpyF3I	CTNAG	8 cut(s)	177, 306, 600, 729, 813, 861, 1066, 1240
HpySE526I	ACGT	2 cut(s)	286, 448
Hsp92II	CATG	4 cut(s)	176, 1005, 1571, 1626
HspAI	GCGC	1 cut(s)	420
Ksp22I	TGATCA	2 cut(s)	169, 661
LmnI	GCTCC	5 cut(s)	226, 748, 760, 1236, 1474
Lsp1109I	GCAGC	5 cut(s)	69, 1231, 1259, 1372, 1654
LweI	GCATC	3 cut(s)	215, 941, 1617
MaeI	CTAG	4 cut(s)	344, 977, 1131, 1653
MaeII	ACGT	2 cut(s)	286, 448
MaeIII	GTNAC	2 cut(s)	666, 1531
MboII	GAAGA	8 cut(s)	260, 413, 434, 616, 619, 710, 745, 1498
MfeI	CAATTG	1 cut(s)	535
MflI	RGATCY	4 cut(s)	949, 955, 986, 1344
MhlI	GDGCHC	1 cut(s)	1067
MlyI	GAGTC	7 cut(s)	115, 652, 824, 866, 908, 974, 1628
MmeI	TCCRAC	5 cut(s)	328, 786, 870, 961, 1463
MslI	CAYNNNNRTG	1 cut(s)	840
MspA1I	CMGCKG	1 cut(s)	82
MspR9I	CCNGG	3 cut(s)	225, 595, 1632
MssI	GTTTAAAC	1 cut(s)	115
MunI	CAATTG	1 cut(s)	535
Mva1269I	GAATGC	1 cut(s)	1748
MvaI	CCWGG	3 cut(s)	225, 595, 1632
MwoI	GCNNNNNNNGC	3 cut(s)	1088, 1385, 1797
NdeI	CATATG	1 cut(s)	16
NlaIII	CATG	4 cut(s)	176, 1005, 1571, 1626
NlaIV	GGNNCC	3 cut(s)	957, 1346, 1638
NmuCI	GTSAC	1 cut(s)	666
NsbI	TGCGCA	1 cut(s)	421
PagI	TCATGA	1 cut(s)	1567
PcsI	WCGNNNNNNNCGW	1 cut(s)	1345
PctI	GAATGC	1 cut(s)	1748
PfeI	GAWTC	8 cut(s)	70, 541, 809, 865, 998, 1016, 1175, 1577
PflMI	CCANNNNNTGG	1 cut(s)	1528
PkrI	GCNGC	8 cut(s)	84, 1246, 1249, 1286, 1387, 1390, 1669, 1672
PleI	GAGTC	7 cut(s)	114, 651, 823, 865, 907, 973, 1627
PmeI	GTTTAAAC	1 cut(s)	115
PpsI	GAGTC	7 cut(s)	114, 651, 823, 865, 907, 973, 1627
Ppu21I	YACGTR	1 cut(s)	287
Psp124BI	GAGCTC	1 cut(s)	1067
Psp6I	CCWGG	3 cut(s)	223, 593, 1630
PspGI	CCWGG	3 cut(s)	223, 593, 1630
PspN4I	GGNNCC	3 cut(s)	957, 1346, 1638
PspPI	GGNCC	2 cut(s)	1055, 1756
PstI	CTGCAG	2 cut(s)	87, 1249
PstNI	CAGNNNCTG	2 cut(s)	860, 1736
PsuI	RGATCY	4 cut(s)	949, 955, 986, 1344
PvuII	CAGCTG	1 cut(s)	82
RsaI	GTAC	4 cut(s)	229, 237, 906, 1351
RsaNI	GTAC	4 cut(s)	228, 236, 905, 1350
RseI	CAYNNNNRTG	1 cut(s)	840
SacI	GAGCTC	1 cut(s)	1067
SatI	GCNGC	8 cut(s)	83, 1245, 1248, 1285, 1386, 1389, 1668, 1671
Sau96I	GGNCC	2 cut(s)	1055, 1756
SchI	GAGTC	7 cut(s)	115, 652, 824, 866, 908, 974, 1628
ScrFI	CCNGG	3 cut(s)	225, 595, 1632
SduI	GDGCHC	1 cut(s)	1067
SfaNI	GCATC	3 cut(s)	215, 941, 1617
SfcI	CTRYAG	5 cut(s)	83, 139, 525, 1137, 1245
SinI	GGWCC	1 cut(s)	1055
SmiMI	CAYNNNNRTG	1 cut(s)	840
SmlI	CTYRAG	2 cut(s)	101, 1616
SmoI	CTYRAG	2 cut(s)	101, 1616
SpeI	ACTAGT	1 cut(s)	343
SsiI	CCGC	7 cut(s)	128, 239, 530, 1284, 1388, 1670, 1778
SspI	AATATT	1 cut(s)	7
SspMI	CTAG	4 cut(s)	344, 977, 1131, 1653
SstI	GAGCTC	1 cut(s)	1067
StyD4I	CCNGG	3 cut(s)	223, 593, 1630
StyI	CCWWGG	2 cut(s)	1469, 1759
TaaI	ACNGT	8 cut(s)	143, 298, 461, 771, 786, 881, 1171, 1356
TaiI	ACGT	2 cut(s)	289, 451
TaqI	TCGA	5 cut(s)	649, 675, 1033, 1339, 1823
TauI	GCSGC	3 cut(s)	1287, 1391, 1673
TfiI	GAWTC	8 cut(s)	70, 541, 809, 865, 998, 1016, 1175, 1577
TscAI	CASTG	9 cut(s)	45, 92, 102, 148, 303, 314, 466, 1205, 1819
TseFI	GTSAC	1 cut(s)	666
TseI	GCWGC	5 cut(s)	82, 1244, 1247, 1385, 1667
Tsp45I	GTSAC	1 cut(s)	666
TspDTI	ATGAA	8 cut(s)	17, 735, 858, 899, 1515, 1517, 1557, 1658
TspGWI	ACGGA	2 cut(s)	1337, 1441
TspRI	CASTG	9 cut(s)	45, 92, 102, 148, 303, 314, 466, 1205, 1819
Van91I	CCANNNNNTGG	1 cut(s)	1528
VpaK11BI	GGWCC	1 cut(s)	1055
XapI	RAATTY	3 cut(s)	152, 1266, 1599
XmiI	GTMKAC	1 cut(s)	818
XspI	CTAG	4 cut(s)	344, 977, 1131, 1653

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.