Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003457

Physical Location & Seq

Genomic Coordinates Forward (+)

16570597 .. 16571953

Gene Structure 1357 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1077600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 741 bp

ATGTCTCTGTCCAACGACGACGACAGAAAGGTCTTCGGAATTGTTTTCCGAACACCGCCGATCGATTCGACTGGAGTCGCTGACATACTGCAGCAGAGTGTGTTATGTGGGTCAAGAAAGTACCCCGTGAAAAAACCATTTGATGAGTTGGTTAGAGGGAGCTTCAGTACTTCTCTGCATGCTAAAACAATGGCTGATAGGACCTACTACTCTGGTTCTTGCACAACTACAAAGGAATTGTATAATATGGTTGATGTACACCTTGATGCCGTTTTCTTTCCTAAATGTTTGGAGGACGTCCAGATTTTCCAGCAACAGGGTTGGCATTACGAGCTCAATATCTTATCAAGGAAAGAGTTCTATTCTCGGCCTTTGCCTGAAGATATACTGAAGCTGACTGCTCAACAGGCAAGCTTTCCTTCTACTCTTTCGTCCAATCGTTTATTGTTTATGTTTGTGGACTTGTTAATGCTGAACACGAGTACAAAAGACATGAATTTTATTCAACTTCATGAATTGGTTAAAAGAAAAACAGGAGGAATATCAATTGATCCAATGACGTCATCTGTGCGGTGCAAGAAAGATCCCTGTGGCCATATAGTTGTTCGAGGGAAAGTCATGGCAGGACGTGCTGAAGACCTATTTGAACTGGGACTGGGAATTCGAACTTGGATGAAGCGGGCTCCATCCAGCAAAACTCCTGCCTTATTCAAAAATTTAAAAAACTCAAAGAAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0010035 GO:0010038 GO:0010467 GO:0016485 GO:0016787 GO:0019538 GO:0031967 GO:0031974 GO:0031975 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:0051604 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

247

Amino Acids

27.88

Weight (kDa)

9.4

Isoelectric Point (pI)

43.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
M16C_assoc	PF08367	148 - 217	2.9e-07	Peptidase M16C associated

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003457)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g30421
malus_domestica	MD05G1077600.v1.1 MD05G1078300.v1.1 MD05G1078500.v1.1
rosa_chinensis	RchiOBHm_Chr3g0496491 RchiOBHm_Chr5g0052551 RchiOBHm_Chr6g0274301
rosa_laevigata	RLG00000024686
rosa_multiflora	Rmu_co8446175.1_g000001 Rmu_sc0003641.1_g000017 Rmu_sc0004230.1_g000016
rosa_roxburghii	Rroxscaffold_1G00032360 Rroxscaffold_1G00038920 Rroxscaffold_4G00320760 Rroxscaffold_7G00201650
rosa_rugosa	Rorug05G0348000 Rorug07G0144800 Rorug07G0144900
rosa_samantha	Rh1AG009800 Rh1AG099800 Rh1AG100700 Rh1AG144900 Rh1CG204500 Rh1DG101900 Rh2AG517000 Rh2BG259000 Rh2DG439900 Rh3CG357900 Rh4CG191000 Rh4CG244300 Rh6AG274200 Rh6CG030900 Rh6CG098900 Rh6CG276500 Rh7AG405200 Rh7CG299500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	29
AatII	GACGTC	2 cut(s)	300, 563
AciI	CCGC	3 cut(s)	56, 571, 679
AclWI	GGATC	2 cut(s)	545, 578
AcoI	YGGCCR	1 cut(s)	592
AcsI	RAATTY	4 cut(s)	496, 660, 715, 735
AcuI	CTGAAG	4 cut(s)	148, 399, 410, 654
AcyI	GRCGYC	2 cut(s)	297, 560
AfaI	GTAC	4 cut(s)	122, 169, 258, 484
AfiI	CCNNNNNNNGG	1 cut(s)	316
AgsI	TTSAA	3 cut(s)	506, 647, 712
AjiI	CACGTC	1 cut(s)	629
AjuI	GAANNNNNNNTTGG	2 cut(s)	652, 684
AluBI	AGCT	4 cut(s)	162, 334, 394, 414
AluI	AGCT	4 cut(s)	162, 334, 394, 414
Alw21I	GWGCWC	1 cut(s)	336
Alw26I	GTCTC	1 cut(s)	9
AlwI	GGATC	2 cut(s)	545, 578
AoxI	GGCC	2 cut(s)	368, 592
ApeKI	GCWGC	1 cut(s)	91
ApoI	RAATTY	4 cut(s)	496, 660, 715, 735
ArsI	GACNNNNNNTTYG	2 cut(s)	645, 677
Asp700I	GAANNNNTTC	1 cut(s)	356
AspS9I	GGNCC	1 cut(s)	201
AsuII	TTCGAA	1 cut(s)	664
AvaII	GGWCC	1 cut(s)	201
BalI	TGGCCA	1 cut(s)	594
BanII	GRGCYC	2 cut(s)	336, 685
BauI	CACGAG	1 cut(s)	478
BbsI	GAAGAC	2 cut(s)	25, 642
Bbv12I	GWGCWC	1 cut(s)	336
BbvI	GCAGC	1 cut(s)	103
BccI	CCATC	1 cut(s)	694
BceAI	ACGGC	1 cut(s)	254
BcoDI	GTCTC	1 cut(s)	9
BfmI	CTRYAG	1 cut(s)	89
BisI	GCNGC	1 cut(s)	92
BlsI	GCNGC	1 cut(s)	93
BmcAI	AGTACT	1 cut(s)	169
Bme18I	GGWCC	1 cut(s)	201
BmgBI	CACGTC	1 cut(s)	629
BmgT120I	GGNCC	1 cut(s)	201
BmiI	GGNNCC	1 cut(s)	684
BmrI	ACTGGG	2 cut(s)	659, 665
BmsI	GCATC	1 cut(s)	256
BmuI	ACTGGG	2 cut(s)	659, 665
BoxI	GACNNNNGTC	1 cut(s)	74
BpiI	GAAGAC	2 cut(s)	25, 642
BpmI	CTGGAG	1 cut(s)	93
Bpu14I	TTCGAA	1 cut(s)	664
Bsa29I	ATCGAT	1 cut(s)	63
BsaHI	GRCGYC	2 cut(s)	297, 560
BsaXI	ACNNNNNCTCC	2 cut(s)	151, 181
Bsc4I	CCNNNNNNNGG	1 cut(s)	316
Bse1I	ACTGG	3 cut(s)	76, 654, 660
BseCI	ATCGAT	1 cut(s)	63
BseGI	GGATG	2 cut(s)	678, 686
BseLI	CCNNNNNNNGG	1 cut(s)	316
BseNI	ACTGG	3 cut(s)	76, 654, 660
BseXI	GCAGC	1 cut(s)	103
Bsh1285I	CGRYCG	1 cut(s)	63
BshFI	GGCC	2 cut(s)	370, 594
BshVI	ATCGAT	1 cut(s)	63
BsiEI	CGRYCG	1 cut(s)	63
BsiHKAI	GWGCWC	1 cut(s)	336
BslFI	GGGAC	1 cut(s)	666
BslI	CCNNNNNNNGG	1 cut(s)	316
BsmAI	GTCTC	1 cut(s)	9
BsmFI	GGGAC	1 cut(s)	666
BsnI	GGCC	2 cut(s)	370, 594
Bsp119I	TTCGAA	1 cut(s)	664
Bsp1286I	GDGCHC	2 cut(s)	336, 685
Bsp1407I	TGTACA	1 cut(s)	256
Bsp143I	GATC	3 cut(s)	60, 550, 583
BspACI	CCGC	3 cut(s)	56, 571, 679
BspANI	GGCC	2 cut(s)	370, 594
BspDI	ATCGAT	1 cut(s)	63
BspHI	TCATGA	1 cut(s)	511
BspLI	GGNNCC	1 cut(s)	684
BspMAI	CTGCAG	1 cut(s)	93
BspPI	GGATC	2 cut(s)	545, 578
BspT104I	TTCGAA	1 cut(s)	664
BsrGI	TGTACA	1 cut(s)	256
BsrI	ACTGG	3 cut(s)	76, 654, 660
BssMI	GATC	3 cut(s)	60, 550, 583
BssNI	GRCGYC	2 cut(s)	297, 560
BssSI	CACGAG	1 cut(s)	478
Bst2BI	CACGAG	1 cut(s)	478
BstACI	GRCGYC	2 cut(s)	297, 560
BstAPI	GCANNNNNTGC	1 cut(s)	629
BstAUI	TGTACA	1 cut(s)	256
BstBI	TTCGAA	1 cut(s)	664
BstC8I	GCNNGC	3 cut(s)	180, 412, 681
BstF5I	GGATG	2 cut(s)	678, 686
BstKTI	GATC	3 cut(s)	63, 553, 586
BstMAI	GTCTC	1 cut(s)	9
BstMBI	GATC	3 cut(s)	60, 550, 583
BstMCI	CGRYCG	1 cut(s)	63
BstMWI	GCNNNNNNNGC	3 cut(s)	331, 407, 629
BstNSI	RCATGY	1 cut(s)	182
BstPAI	GACNNNNGTC	1 cut(s)	74
BstSFI	CTRYAG	1 cut(s)	89
BstV1I	GCAGC	1 cut(s)	103
BstV2I	GAAGAC	2 cut(s)	25, 642
BstX2I	RGATCY	1 cut(s)	583
BstYI	RGATCY	1 cut(s)	583
Bsu15I	ATCGAT	1 cut(s)	63
BsuRI	GGCC	2 cut(s)	370, 594
BsuTUI	ATCGAT	1 cut(s)	63
BtrI	CACGTC	1 cut(s)	629
BtsCI	GGATG	2 cut(s)	678, 686
Cac8I	GCNNGC	3 cut(s)	180, 412, 681
CciI	TCATGA	1 cut(s)	511
Cfr13I	GGNCC	1 cut(s)	201
ClaI	ATCGAT	1 cut(s)	63
Csp6I	GTAC	4 cut(s)	121, 168, 257, 483
CviAII	CATG	4 cut(s)	179, 493, 512, 619
CviJI	RGCY	8 cut(s)	162, 194, 334, 370, 394, 414, 594, 683
CviKI_1	RGCY	8 cut(s)	162, 194, 334, 370, 394, 414, 594, 683
CviQI	GTAC	4 cut(s)	121, 168, 257, 483
DpnI	GATC	3 cut(s)	62, 552, 585
DpnII	GATC	3 cut(s)	60, 550, 583
DraI	TTTAAA	1 cut(s)	720
DrdI	GACNNNNNNGTC	1 cut(s)	29
DseDI	GACNNNNNNGTC	1 cut(s)	29
EaeI	YGGCCR	1 cut(s)	592
Ecl136II	GAGCTC	1 cut(s)	334
Eco24I	GRGCYC	2 cut(s)	336, 685
Eco47I	GGWCC	1 cut(s)	201
Eco53kI	GAGCTC	1 cut(s)	334
Eco57I	CTGAAG	4 cut(s)	148, 399, 410, 654
EcoICRI	GAGCTC	1 cut(s)	334
EcoO109I	RGGNCCY	1 cut(s)	201
EcoRI	GAATTC	1 cut(s)	660
EcoT38I	GRGCYC	2 cut(s)	336, 685
FaeI	CATG	4 cut(s)	182, 496, 515, 622
FalI	AAGNNNNNCTT	2 cut(s)	403, 435
FaqI	GGGAC	1 cut(s)	666
FatI	CATG	4 cut(s)	178, 492, 511, 618
FauI	CCCGC	1 cut(s)	672
Fnu4HI	GCNGC	1 cut(s)	92
FokI	GGATG	2 cut(s)	673, 685
FriOI	GRGCYC	2 cut(s)	336, 685
Fsp4HI	GCNGC	1 cut(s)	92
GluI	GCNGC	1 cut(s)	92
GsuI	CTGGAG	1 cut(s)	93
HaeIII	GGCC	2 cut(s)	370, 594
Hin1I	GRCGYC	2 cut(s)	297, 560
Hin1II	CATG	4 cut(s)	182, 496, 515, 622
HindIII	AAGCTT	1 cut(s)	412
HinfI	GANTC	2 cut(s)	65, 75
Hpy166II	GTNNAC	2 cut(s)	259, 460
Hpy188I	TCNGA	2 cut(s)	38, 50
Hpy188III	TCNNGA	3 cut(s)	114, 301, 512
Hpy8I	GTNNAC	2 cut(s)	259, 460
Hpy99I	CGWCG	2 cut(s)	20, 23
HpyAV	CCTTC	1 cut(s)	429
HpyCH4IV	ACGT	3 cut(s)	297, 560, 628
HpyCH4V	TGCA	4 cut(s)	91, 178, 222, 576
HpyF10VI	GCNNNNNNNGC	3 cut(s)	331, 407, 629
HpySE526I	ACGT	3 cut(s)	297, 560, 628
Hsp92I	GRCGYC	2 cut(s)	297, 560
Hsp92II	CATG	4 cut(s)	182, 496, 515, 622
Kzo9I	GATC	3 cut(s)	60, 550, 583
LmnI	GCTCC	2 cut(s)	159, 688
Lsp1109I	GCAGC	1 cut(s)	103
LweI	GCATC	1 cut(s)	256
MaeII	ACGT	3 cut(s)	297, 560, 628
MalI	GATC	3 cut(s)	62, 552, 585
MboI	GATC	3 cut(s)	60, 550, 583
MboII	GAAGA	3 cut(s)	25, 392, 647
MfeI	CAATTG	1 cut(s)	546
MflI	RGATCY	1 cut(s)	583
MhlI	GDGCHC	2 cut(s)	336, 685
MlsI	TGGCCA	1 cut(s)	594
MluCI	AATT	8 cut(s)	39, 236, 496, 515, 546, 660, 715, 735
MluNI	TGGCCA	1 cut(s)	594
MlyI	GAGTC	1 cut(s)	84
MmeI	TCCRAC	1 cut(s)	36
MnlI	CCTC	4 cut(s)	149, 286, 530, 602
Mox20I	TGGCCA	1 cut(s)	594
MroXI	GAANNNNTTC	1 cut(s)	356
MscI	TGGCCA	1 cut(s)	594
MseI	TTAA	3 cut(s)	467, 522, 719
MslI	CAYNNNNRTG	1 cut(s)	264
Msp20I	TGGCCA	1 cut(s)	594
MunI	CAATTG	1 cut(s)	546
MwoI	GCNNNNNNNGC	3 cut(s)	331, 407, 629
NdeII	GATC	3 cut(s)	60, 550, 583
NlaIII	CATG	4 cut(s)	182, 496, 515, 622
NlaIV	GGNNCC	1 cut(s)	684
NmeAIII	GCCGAG	1 cut(s)	346
NspI	RCATGY	1 cut(s)	182
NspV	TTCGAA	1 cut(s)	664
PaeI	GCATGC	1 cut(s)	182
PagI	TCATGA	1 cut(s)	511
PdmI	GAANNNNTTC	1 cut(s)	356
PfeI	GAWTC	1 cut(s)	65
PkrI	GCNGC	1 cut(s)	93
Ple19I	CGATCG	1 cut(s)	63
PleI	GAGTC	1 cut(s)	83
PpsI	GAGTC	1 cut(s)	83
PpuMI	RGGWCCY	1 cut(s)	201
PshAI	GACNNNNGTC	1 cut(s)	74
Psp124BI	GAGCTC	1 cut(s)	336
Psp5II	RGGWCCY	1 cut(s)	201
PspN4I	GGNNCC	1 cut(s)	684
PspPI	GGNCC	1 cut(s)	201
PspPPI	RGGWCCY	1 cut(s)	201
PstI	CTGCAG	1 cut(s)	93
PsuI	RGATCY	1 cut(s)	583
PvuI	CGATCG	1 cut(s)	63
RsaI	GTAC	4 cut(s)	122, 169, 258, 484
RsaNI	GTAC	4 cut(s)	121, 168, 257, 483
RseI	CAYNNNNRTG	1 cut(s)	264
SacI	GAGCTC	1 cut(s)	336
SaqAI	TTAA	3 cut(s)	467, 522, 719
SatI	GCNGC	1 cut(s)	92
Sau3AI	GATC	3 cut(s)	60, 550, 583
Sau96I	GGNCC	1 cut(s)	201
ScaI	AGTACT	1 cut(s)	169
SchI	GAGTC	1 cut(s)	84
SduI	GDGCHC	2 cut(s)	336, 685
SfaNI	GCATC	1 cut(s)	256
SfcI	CTRYAG	1 cut(s)	89
SfuI	TTCGAA	1 cut(s)	664
SinI	GGWCC	1 cut(s)	201
SmiMI	CAYNNNNRTG	1 cut(s)	264
SphI	GCATGC	1 cut(s)	182
Sse9I	AATT	8 cut(s)	39, 236, 496, 515, 546, 660, 715, 735
SsiI	CCGC	3 cut(s)	56, 571, 679
SstI	GAGCTC	1 cut(s)	336
TaiI	ACGT	3 cut(s)	300, 563, 631
TaqI	TCGA	4 cut(s)	63, 68, 607, 664
TasI	AATT	8 cut(s)	39, 236, 496, 515, 546, 660, 715, 735
TatI	WGTACW	3 cut(s)	167, 256, 482
TfiI	GAWTC	1 cut(s)	65
Tru1I	TTAA	3 cut(s)	467, 522, 719
Tru9I	TTAA	3 cut(s)	467, 522, 719
TseI	GCWGC	1 cut(s)	91
TspDTI	ATGAA	4 cut(s)	500, 509, 528, 689
VpaK11BI	GGWCC	1 cut(s)	201
XapI	RAATTY	4 cut(s)	496, 660, 715, 735
XceI	RCATGY	1 cut(s)	182
XmnI	GAANNNNTTC	1 cut(s)	356
ZraI	GACGTC	2 cut(s)	298, 561
ZrmI	AGTACT	1 cut(s)	169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.