Rorug07G0144900

Pleiotropic drug resistance protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003457
Physical Location & Seq
Forward (+)
11422786 .. 11429480
6695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0144900.1

Sequence Viewer

Length: 3000 bp
ATGGAGAATGCAGTGGGTGGGGAAGCCACCCCACCTAATTCGACGGAGGGTGCACAGCTTCAGAGCAAGGAGAACGGGCATGCATTGAGACATGGAAATGACCACATGTTCGACTACCAAGAGATGCGTATACCGGGTGGTGACGAAGATCATTCCCTGCGTATACCAGTTGGAAGCAATTGTCCTGAGAGCTCAAGTCAGGAGTGTTTTGTAAAACAACCGTATGATGGCTTAGGCTTATATCCAAGCGGATCAAGGAGCAGCGGGAATGGTGGGAGTTCACGAGTCAATACTGCATTCAGAGATTATGGTCAGATGAGGTCAAATGATTTGGAGAAAGTGGAGTACCCATCTCTGGATGATATTATGGCTCAGAGGGCAAATAGTGATAATCTGGTTGAAGTTGTGGCGGAATTGACAAATACTGTAGATGGAGGGGTTTCAGGCAATTCATATGGAGGTATCCGGACAAAAATGCTTTCCAAATCAAGATCAGAATTTTATGTTAAGTCTACATTGAAGGGTAAGGGTGTTATTTTCAAAGGTCCAAATCATGATGGCGGTCATGTTGAATCTAAGAACCTTAACAGTACAAAGGTTACTACTGGTGGTTCTAAGGCTACTTCTGATTCAATACAGAGTTTGGATGCCAAAATTGGTATGCCTTCTTCCAATGATGTGAGCGTTGTGCCCAAACCACCAGCCTTGGAACATGATGAAATCAGTTTAAGAGAATGGCTGAGAATTGGACGTCCTAATAAAGTCAATAAAGTTGAGTGTCTGTATGTATTCAAACAAATTGCAAATCTCGTGGATCATTTTCACTCTCAAGGAGTTGCTTTGCAAGAATTGCGCCCTTCTTTTTTTAAGGTGTCACCTTCAAATGAGGTCAAGTATGTTGGCTTACCAATCCCAAAAGAGATATTAGAAAGTTCTATGGATCAAGGTATCTGTCATTCAGAAAATAGTCCAATGAGAAAAATGTCAGGTGAGCAAGAATTTGCTTCTGTTGGTTCCAGTGCAAAGAAGCAAAAGATCAGCCAGAACACTAGATTAGTTAGGCAATGGCCTCAGTTCCCCACTAGTTCCCGTTTCCAACGTGAAACTAGGAATACTAGCCACATCAACATCAATGGTCTGCAAAATAGAAGTCCTCATATGTATAATGCAGCTCAACAACACTTGACTTCAATCACTGAGCAATTGGAAGAAAAGTGGTATATTAGTCCTGAAGAACTCAATGAAGGGAGCTGTACAGTTTTCTCAAATATCTACAGTCTGGGCGTTCTTCTTTTTGAGTTGCTGGCCCGTTTTGACTCAAATAGTGCCGTTGTCTCAGCAATGTTCAGTCTGCGTCATAGAATTCTTCCTCCGAATTTTCTGTCAGAAAACGCTAAAGAGGCTGGATTTTGTCTTTGGTTACTTCATCCTGAACCGTCTTCACGTCCAACAACAAGGGAAATTCTACAATCAGAAGTAGTTAATGGATTACAGGAGATGTGTGAAGAGAAAGTGGCATCATCTGTGGTCGAAGAGGATGCTGAATTGGAACTATTATCCCATTTCCTCACGTCACTGAAAGAAAAGAAGCAGAAGGATGCCTCCAAGTTGATGGCAAGCATTAGGGTTTTAGAAGCAGATGCTGAAGAAGCTGAGAGGAGGCACTATTCAAGAAATCCCGACTGGTACAAAGAGTCCCTCGGTGGATGGAAAAACGCATGTAAAGAAGCTTCAAGATCAGAGGTGCTTTCTCCGTTACCCCCTCTTCCTAGTTCAAATGAGTCAAGGTTGACGAGGAATGTTGATCAGCTTGAGAGTGCTTACTTCTCTATGAGATCTATGATTCAGCCTCCTGAAGCTGATTGTAATATACGCATGGATAAAGAGTTATTAAAATCTCGCAAGAATTGGTATTTGGAATCAGATGATGACGAAAAGAAGACTCCTACTGATCAATTGGGAGCTATCTTTGATGGTTTATGCAAGTATGATCGGTACAGTAAGTTTGAAGTATGTGGAGTACTAAGAAATGGAGATTTCAACAGTAACTCAAATTTGATTTGCTCTTTGAGTTTTGATCGAGATGAGGACTACATCGCTTCTGCTGGGGTTTCAAAGAAAATAAAGATTTATGAGTTCAATGCACTTTTCAATGATTCTGTTGATATTCATTATCCATCGATTGAGATGTCAAATGAATCAAAGATTAGCTGTATTTGCTGGAACAACTACATAAAAAACTATCTCACTTCAACTGATTATGAAGGTGTAGTGAAGTTGTGGGATGTCAGCACTGGTCAAGAGTTTGCTCAATACAAAGGGCATGAACAAAAGGCTTGGTCTGTTGACTTCTCTCAAGTAGATCCTACAAAATTAGCCAGCGGGAGTGATGACGGTTCTGTGAAACTCTGGAGCATTAATGAGAAAAACTGTTTAGGCACCATCAGAAACAGAAACATTGCAAATGTATGCTGTGTACAGTTCTCTGCTCACTCCACTAATTTGCTGGCCTTTGGGTCTGCGGACTACAACACATATTGCTATGATATTCGTAATGCTAGAACGCCCTGGTGTGTATTGGTTGGTCACCAAAAAAGCGTAAGCTATGTCAAATTCTTGGACTCTGAGACCCTTGTTTCTGCATCCACTGACAACACATTAAAGCTATGGGATCTCAACAAAACCACTGCCTGTGGTCCACCCACCAATGCTTGCAGCTTAACCCTCAGTGGGCATACCAATGAGAAGAACTTTGTGGGCTTATCCGTTTCTAATGGTTATATAGCATGCGGTTCAGAAACAAATGAGGTCTATGCTTATTATAGATCTCTGCCTATGCCGATCACTTCATACAAATTTGGCTCCATTGATCCTATAACCGGAAAGGAGACTGATGATATTAATGGATCATTTGTTTCAAGTGTCTGCTGGAGACAGAAATCAAACATGGTGGTTGCCGCCAATTCAAGCGGGTGCATAAAAGTGTTGCAGATTGTTTGA

Protein Analysis

999

Amino Acids

111.25

Weight (kDa)

5.76

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 398 - 492 5.9e-06 Protein kinase domain
Beta-prop_WDR5 PF25175 683 - 940 1e-32 WDR5 beta-propeller domain
WDR55 PF24796 685 - 816 5.2e-07 WDR55
Beta-prop_TEP1_2nd PF25047 686 - 805 4.9e-06 TEP-1 second beta-propeller
Beta-prop_EML PF23409 687 - 894 1e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 687 - 852 9.4e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 689 - 808 9.1e-07 WDR3 second beta-propeller domain
Beta-prop_RIG_2nd PF23775 694 - 784 2.9e-08 RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 698 - 890 1.4e-13 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 730 - 845 3.5e-16 THOC3 beta-propeller domain
WD40_Prp19 PF24814 731 - 818 1.1e-08 Prp19 WD40 domain
Beta-prop_WDR19_1st PF23389 739 - 895 1e-06 WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 747 - 896 7.1e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 750 - 896 5.6e-12 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 751 - 838 1.1e-07 EIF3I
WD40_WDHD1_1st PF24817 758 - 815 8.1e-06 WDHD1 first WD40 domain
WD40 PF00400 769 - 805 5.4e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 778 - 896 2.9e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 790 - 940 6.1e-20 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 792 - 896 4.3e-11 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 818 - 993 3e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 821 - 933 2.6e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 823 - 997 2.4e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 754
AccB1I GGYRCC 1 cut(s) 2438
AccI GTMKAC 3 cut(s) 130, 163, 512
AccIII TCCGGA 1 cut(s) 465
AciI CCGC 9 cut(s) 249, 264, 410, 561, 2382, 2522, 2790, 2958, 2970
AclWI GGATC 7 cut(s) 259, 822, 948, 2357, 2679, 2864, 2914
AcsI RAATTY 8 cut(s) 497, 998, 1362, 1375, 1461, 2053, 2612, 2855
AcuI CTGAAG 4 cut(s) 44, 1251, 1665, 1875
AcyI GRCGYC 1 cut(s) 751
AfaI GTAC 7 cut(s) 347, 592, 1255, 1688, 1997, 2022, 2478
AfiI CCNNNNNNNGG 4 cut(s) 227, 355, 2730, 2879
AflIII ACRYGT 1 cut(s) 105
AhlI ACTAGT 1 cut(s) 1082
AjiI CACGTC 2 cut(s) 1445, 1572
AjnI CCWGG 1 cut(s) 2567
AjuI GAANNNNNNNTTGG 2 cut(s) 1898, 1930
Alw21I GWGCWC 2 cut(s) 55, 194
Alw26I GTCTC 5 cut(s) 82, 1339, 2621, 2882, 2926
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 7 cut(s) 259, 822, 948, 2357, 2679, 2864, 2914
AlwNI CAGNNNCTG 1 cut(s) 1643
Aor13HI TCCGGA 1 cut(s) 465
AoxI GGCC 3 cut(s) 1067, 1305, 2508
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 3 cut(s) 261, 1169, 2715
ApoI RAATTY 8 cut(s) 497, 998, 1362, 1375, 1461, 2053, 2612, 2855
ArsI GACNNNNNNTTYG 6 cut(s) 92, 124, 1135, 1167, 1367, 1399
AseI ATTAAT 2 cut(s) 2418, 2901
AspLEI GCGC 1 cut(s) 855
AspS9I GGNCC 3 cut(s) 545, 1306, 2696
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 4 cut(s) 152, 867, 1001, 2579
AvaII GGWCC 2 cut(s) 545, 2696
BaeGI GKGCMC 2 cut(s) 55, 693
BanI GGYRCC 1 cut(s) 2438
BanII GRGCYC 1 cut(s) 194
BarI GAAGNNNNNNTAC 4 cut(s) 652, 684, 1714, 1746
BauI CACGAG 2 cut(s) 282, 809
BbsI GAAGAC 2 cut(s) 1431, 1946
Bbv12I GWGCWC 2 cut(s) 55, 194
BbvI GCAGC 3 cut(s) 273, 1181, 2727
BccI CCATC 9 cut(s) 221, 358, 425, 551, 1606, 1701, 1967, 2185, 2450
BceAI ACGGC 1 cut(s) 1313
BcgI CGANNNNNNTGC 2 cut(s) 1520, 1554
BciT130I CCWGG 1 cut(s) 2569
BciVI GTATCC 1 cut(s) 473
BclI TGATCA 2 cut(s) 1804, 1951
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 5 cut(s) 82, 1339, 2621, 2882, 2926
BcuI ACTAGT 1 cut(s) 1082
BfaI CTAG 6 cut(s) 1050, 1083, 1107, 1116, 1770, 2559
BfmI CTRYAG 2 cut(s) 426, 1273
BfuI GTATCC 1 cut(s) 473
BglII AGATCT 2 cut(s) 1835, 2825
BisI GCNGC 4 cut(s) 262, 1170, 2716, 2958
BlsI GCNGC 4 cut(s) 263, 1171, 2717, 2959
BmcAI AGTACT 1 cut(s) 2022
Bme1390I CCNGG 2 cut(s) 135, 2569
Bme18I GGWCC 2 cut(s) 545, 2696
BmgBI CACGTC 2 cut(s) 1445, 1572
BmgT120I GGNCC 3 cut(s) 545, 1306, 2696
BmiI GGNNCC 3 cut(s) 1015, 2440, 2863
BmrFI CCNGG 2 cut(s) 135, 2569
BmsI GCATC 7 cut(s) 114, 637, 1526, 1528, 1588, 1630, 2651
BpiI GAAGAC 2 cut(s) 1431, 1946
BpmI CTGGAG 2 cut(s) 2431, 2950
Bpu10I CCTNAGC 1 cut(s) 232
BpuEI CTTGAG 4 cut(s) 178, 813, 1832, 2340
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 2180
BsaHI GRCGYC 1 cut(s) 751
BsaI GGTCTC 1 cut(s) 2621
BsaJI CCNNGG 3 cut(s) 705, 1699, 2567
BsaWI WCCGGW 2 cut(s) 465, 2879
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 355, 2730, 2879
Bse1I ACTGG 5 cut(s) 167, 610, 1017, 1688, 2299
Bse3DI GCAATG 3 cut(s) 1070, 1347, 2457
BseAI TCCGGA 1 cut(s) 465
BseBI CCWGG 1 cut(s) 2569
BseCI ATCGAT 1 cut(s) 2180
BseDI CCNNGG 3 cut(s) 705, 1699, 2567
BseGI GGATG 8 cut(s) 364, 652, 1426, 1543, 1603, 1712, 2290, 2642
BseLI CCNNNNNNNGG 4 cut(s) 227, 355, 2730, 2879
BseMI GCAATG 3 cut(s) 1070, 1347, 2457
BseMII CTCAG 9 cut(s) 177, 386, 731, 1085, 1188, 1350, 1644, 2616, 2740
BseNI ACTGG 5 cut(s) 167, 610, 1017, 1688, 2299
BseRI GAGGAG 1 cut(s) 1672
BseSI GKGCMC 2 cut(s) 55, 693
BseXI GCAGC 3 cut(s) 273, 1181, 2727
BseYI CCCAGC 1 cut(s) 2105
BshFI GGCC 3 cut(s) 1069, 1307, 2510
BshNI GGYRCC 1 cut(s) 2438
BshVI ATCGAT 1 cut(s) 2180
BsiHKAI GWGCWC 2 cut(s) 55, 194
BsiSI CCGG 3 cut(s) 134, 466, 2880
BslFI GGGAC 1 cut(s) 1681
BslI CCNNNNNNNGG 4 cut(s) 227, 355, 2730, 2879
BsmAI GTCTC 5 cut(s) 82, 1339, 2621, 2882, 2926
BsmFI GGGAC 1 cut(s) 1681
BsmI GAATGC 2 cut(s) 13, 296
BsnI GGCC 3 cut(s) 1069, 1307, 2510
Bso31I GGTCTC 1 cut(s) 2621
Bsp1286I GDGCHC 3 cut(s) 55, 194, 693
Bsp13I TCCGGA 1 cut(s) 465
Bsp1407I TGTACA 2 cut(s) 1253, 2476
BspACI CCGC 9 cut(s) 249, 264, 410, 561, 2382, 2522, 2790, 2958, 2970
BspANI GGCC 3 cut(s) 1069, 1307, 2510
BspCNI CTCAG 9 cut(s) 178, 385, 732, 1084, 1189, 1349, 1645, 2617, 2739
BspDI ATCGAT 1 cut(s) 2180
BspEI TCCGGA 1 cut(s) 465
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 3 cut(s) 1015, 2440, 2863
BspPI GGATC 7 cut(s) 259, 822, 948, 2357, 2679, 2864, 2914
BspT107I GGYRCC 1 cut(s) 2438
BspTNI GGTCTC 1 cut(s) 2621
BsrDI GCAATG 3 cut(s) 1070, 1347, 2457
BsrGI TGTACA 2 cut(s) 1253, 2476
BsrI ACTGG 5 cut(s) 167, 610, 1017, 1688, 2299
BssECI CCNNGG 3 cut(s) 705, 1699, 2567
BssNAI GTATAC 2 cut(s) 131, 164
BssNI GRCGYC 1 cut(s) 751
BssSI CACGAG 2 cut(s) 282, 809
BssT1I CCWWGG 1 cut(s) 705
Bst1107I GTATAC 2 cut(s) 131, 164
Bst2BI CACGAG 2 cut(s) 282, 809
Bst2UI CCWGG 1 cut(s) 2569
Bst6I CTCTTC 3 cut(s) 1500, 1527, 1770
BstACI GRCGYC 1 cut(s) 751
BstAPI GCANNNNNTGC 1 cut(s) 850
BstAUI TGTACA 2 cut(s) 1253, 2476
BstC8I GCNNGC 7 cut(s) 81, 1305, 1618, 2380, 2508, 2713, 2788
BstEII GGTNACC 1 cut(s) 2585
BstF5I GGATG 8 cut(s) 364, 652, 1426, 1543, 1603, 1712, 2290, 2642
BstHHI GCGC 1 cut(s) 855
BstMAI GTCTC 5 cut(s) 82, 1339, 2621, 2882, 2926
BstMWI GCNNNNNNNGC 5 cut(s) 377, 850, 1400, 1649, 2217
BstNI CCWGG 1 cut(s) 2569
BstNSI RCATGY 4 cut(s) 83, 109, 1722, 2790
BstPI GGTNACC 1 cut(s) 2585
BstSCI CCNGG 2 cut(s) 133, 2567
BstSFI CTRYAG 2 cut(s) 426, 1273
BstSLI GKGCMC 2 cut(s) 55, 693
BstV1I GCAGC 3 cut(s) 273, 1181, 2727
BstV2I GAAGAC 2 cut(s) 1431, 1946
BstX2I RGATCY 4 cut(s) 1835, 2362, 2671, 2825
BstXI CCANNNNNNTGG 1 cut(s) 1612
BstYI RGATCY 4 cut(s) 1835, 2362, 2671, 2825
BstZ17I GTATAC 2 cut(s) 131, 164
Bsu15I ATCGAT 1 cut(s) 2180
BsuI GTATCC 1 cut(s) 473
BsuRI GGCC 3 cut(s) 1069, 1307, 2510
BsuTUI ATCGAT 1 cut(s) 2180
BtgZI GCGATG 1 cut(s) 2080
BtrI CACGTC 2 cut(s) 1445, 1572
BtsCI GGATG 8 cut(s) 364, 652, 1426, 1543, 1603, 1712, 2290, 2642
BtsI GCAGTG 2 cut(s) 18, 2685
BtsIMutI CAGTG 8 cut(s) 18, 1024, 1194, 1574, 2292, 2646, 2685, 2734
Cac8I GCNNGC 7 cut(s) 81, 1305, 1618, 2380, 2508, 2713, 2788
CaiI CAGNNNCTG 1 cut(s) 1643
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 855
Cfr13I GGNCC 3 cut(s) 545, 1306, 2696
ClaI ATCGAT 1 cut(s) 2180
CseI GACGC 1 cut(s) 1343
Csp6I GTAC 7 cut(s) 346, 591, 1254, 1687, 1996, 2021, 2477
CspCI CAANNNNNGTGG 6 cut(s) 687, 722, 792, 827, 2931, 2966
CviQI GTAC 7 cut(s) 346, 591, 1254, 1687, 1996, 2021, 2477
Eam1104I CTCTTC 3 cut(s) 1500, 1527, 1770
EarI CTCTTC 3 cut(s) 1500, 1527, 1770
EciI GGCGGA 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 705
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 2621
Eco47I GGWCC 2 cut(s) 545, 2696
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 4 cut(s) 44, 1251, 1665, 1875
Eco91I GGTNACC 1 cut(s) 2585
EcoICRI GAGCTC 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 2585
EcoRI GAATTC 1 cut(s) 1362
EcoRII CCWGG 1 cut(s) 2567
EcoT14I CCWWGG 1 cut(s) 705
EcoT22I ATGCAT 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 705
FaqI GGGAC 1 cut(s) 1681
FauI CCCGC 3 cut(s) 257, 2375, 2963
FauNDI CATATG 2 cut(s) 454, 1158
FbaI TGATCA 2 cut(s) 1804, 1951
FblI GTMKAC 3 cut(s) 130, 163, 512
Fnu4HI GCNGC 4 cut(s) 262, 1170, 2716, 2958
FokI GGATG 8 cut(s) 371, 659, 1413, 1550, 1610, 1719, 2297, 2629
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 4 cut(s) 262, 1170, 2716, 2958
FspBI CTAG 6 cut(s) 1050, 1083, 1107, 1116, 1770, 2559
GlaI GCGC 1 cut(s) 854
GluI GCNGC 4 cut(s) 262, 1170, 2716, 2958
GsaI CCCAGC 1 cut(s) 2109
GsuI CTGGAG 2 cut(s) 2431, 2950
HaeIII GGCC 3 cut(s) 1069, 1307, 2510
HapII CCGG 3 cut(s) 134, 466, 2880
HgaI GACGC 1 cut(s) 1343
HhaI GCGC 1 cut(s) 855
Hin1I GRCGYC 1 cut(s) 751
Hin6I GCGC 1 cut(s) 853
HinP1I GCGC 1 cut(s) 853
HincII GTYRAC 2 cut(s) 1791, 2347
HindII GTYRAC 2 cut(s) 1791, 2347
HindIII AAGCTT 1 cut(s) 1728
HpaII CCGG 3 cut(s) 134, 466, 2880
HphI GGTGA 4 cut(s) 152, 867, 1001, 2579
Hpy166II GTNNAC 9 cut(s) 53, 131, 164, 281, 513, 1791, 2347, 2477, 2699
Hpy8I GTNNAC 9 cut(s) 53, 131, 164, 281, 513, 1791, 2347, 2477, 2699
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 7 cut(s) 514, 675, 867, 888, 1238, 1588, 2258
HpyCH4IV ACGT 4 cut(s) 751, 1099, 1444, 1571
HpyF10VI GCNNNNNNNGC 5 cut(s) 377, 850, 1400, 1649, 2217
HpySE526I ACGT 4 cut(s) 751, 1099, 1444, 1571
Hsp92I GRCGYC 1 cut(s) 751
HspAI GCGC 1 cut(s) 853
Kpn2I TCCGGA 1 cut(s) 465
Ksp22I TGATCA 2 cut(s) 1804, 1951
LmnI GCTCC 5 cut(s) 258, 1248, 1961, 2412, 2867
Lsp1109I GCAGC 3 cut(s) 273, 1181, 2727
LweI GCATC 7 cut(s) 114, 637, 1526, 1528, 1588, 1630, 2651
MaeI CTAG 6 cut(s) 1050, 1083, 1107, 1116, 1770, 2559
MaeII ACGT 4 cut(s) 751, 1099, 1444, 1571
MaeIII GTNAC 8 cut(s) 140, 598, 873, 1419, 1572, 1755, 2045, 2585
MfeI CAATTG 3 cut(s) 178, 1202, 1955
MflI RGATCY 4 cut(s) 1835, 2362, 2671, 2825
MhlI GDGCHC 3 cut(s) 55, 194, 693
MlyI GAGTC 6 cut(s) 294, 1310, 1703, 1790, 1936, 2615
MmeI TCCRAC 3 cut(s) 151, 1120, 1472
Mph1103I ATGCAT 1 cut(s) 85
MroI TCCGGA 1 cut(s) 465
MslI CAYNNNNRTG 3 cut(s) 96, 2739, 2981
MspA1I CMGCKG 2 cut(s) 264, 2382
MspI CCGG 3 cut(s) 134, 466, 2880
MspR9I CCNGG 2 cut(s) 135, 2569
MunI CAATTG 3 cut(s) 178, 1202, 1955
Mva1269I GAATGC 2 cut(s) 13, 296
MvaI CCWGG 1 cut(s) 2569
MwoI GCNNNNNNNGC 5 cut(s) 377, 850, 1400, 1649, 2217
NciI CCSGG 1 cut(s) 135
NdeI CATATG 2 cut(s) 454, 1158
NlaIV GGNNCC 3 cut(s) 1015, 2440, 2863
NmuCI GTSAC 4 cut(s) 140, 873, 1572, 2585
NsiI ATGCAT 1 cut(s) 85
NspI RCATGY 4 cut(s) 83, 109, 1722, 2790
PaeI GCATGC 2 cut(s) 83, 2790
PagI TCATGA 1 cut(s) 553
PciI ACATGT 1 cut(s) 105
PctI GAATGC 2 cut(s) 13, 296
PfeI GAWTC 6 cut(s) 572, 629, 1843, 1919, 2156, 2198
PkrI GCNGC 4 cut(s) 263, 1171, 2717, 2959
PleI GAGTC 6 cut(s) 293, 1310, 1702, 1789, 1936, 2615
PpsI GAGTC 6 cut(s) 293, 1310, 1702, 1789, 1936, 2615
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 2 cut(s) 2418, 2901
Psp124BI GAGCTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 2567
PspEI GGTNACC 1 cut(s) 2585
PspFI CCCAGC 1 cut(s) 2105
PspGI CCWGG 1 cut(s) 2567
PspN4I GGNNCC 3 cut(s) 1015, 2440, 2863
PspPI GGNCC 3 cut(s) 545, 1306, 2696
PstNI CAGNNNCTG 1 cut(s) 1643
PsuI RGATCY 4 cut(s) 1835, 2362, 2671, 2825
RsaI GTAC 7 cut(s) 347, 592, 1255, 1688, 1997, 2022, 2478
RsaNI GTAC 7 cut(s) 346, 591, 1254, 1687, 1996, 2021, 2477
RseI CAYNNNNRTG 3 cut(s) 96, 2739, 2981
SacI GAGCTC 1 cut(s) 194
SatI GCNGC 4 cut(s) 262, 1170, 2716, 2958
Sau96I GGNCC 3 cut(s) 545, 1306, 2696
ScaI AGTACT 1 cut(s) 2022
SchI GAGTC 6 cut(s) 294, 1310, 1703, 1790, 1936, 2615
ScrFI CCNGG 2 cut(s) 135, 2569
SduI GDGCHC 3 cut(s) 55, 194, 693
SfaNI GCATC 7 cut(s) 114, 637, 1526, 1528, 1588, 1630, 2651
SfcI CTRYAG 2 cut(s) 426, 1273
SinI GGWCC 2 cut(s) 545, 2696
SmiMI CAYNNNNRTG 3 cut(s) 96, 2739, 2981
SmlI CTYRAG 4 cut(s) 193, 828, 1811, 2355
SmoI CTYRAG 4 cut(s) 193, 828, 1811, 2355
SpeI ACTAGT 1 cut(s) 1082
SphI GCATGC 2 cut(s) 83, 2790
SsiI CCGC 9 cut(s) 249, 264, 410, 561, 2382, 2522, 2790, 2958, 2970
SspMI CTAG 6 cut(s) 1050, 1083, 1107, 1116, 1770, 2559
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 133, 2567
StyI CCWWGG 1 cut(s) 705
TaiI ACGT 4 cut(s) 754, 1102, 1447, 1574
TaqI TCGA 5 cut(s) 41, 111, 1530, 2080, 2180
TatI WGTACW 4 cut(s) 590, 1253, 2020, 2476
TauI GCSGC 1 cut(s) 2960
TfiI GAWTC 6 cut(s) 572, 629, 1843, 1919, 2156, 2198
TscAI CASTG 8 cut(s) 18, 1024, 1201, 1581, 2299, 2653, 2692, 2734
TseFI GTSAC 4 cut(s) 140, 873, 1572, 2585
TseI GCWGC 3 cut(s) 261, 1169, 2715
Tsp45I GTSAC 4 cut(s) 140, 873, 1572, 2585
TspDTI ATGAA 9 cut(s) 441, 732, 1257, 1415, 2159, 2211, 2277, 2340, 2838
TspGWI ACGGA 3 cut(s) 59, 1743, 2755
TspRI CASTG 8 cut(s) 18, 1024, 1201, 1581, 2299, 2653, 2692, 2734
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 2 cut(s) 545, 2696
VspI ATTAAT 2 cut(s) 2418, 2901
XapI RAATTY 8 cut(s) 497, 998, 1362, 1375, 1461, 2053, 2612, 2855
XceI RCATGY 4 cut(s) 83, 109, 1722, 2790
XcmI CCANNNNNNNNNTGG 1 cut(s) 2503
XmiI GTMKAC 3 cut(s) 130, 163, 512
XspI CTAG 6 cut(s) 1050, 1083, 1107, 1116, 1770, 2559
ZraI GACGTC 1 cut(s) 752
ZrmI AGTACT 1 cut(s) 2022
Zsp2I ATGCAT 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.