MD05G1214000.v1.1

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003404
Physical Location & Seq
Forward (+)
34533298 .. 34534537
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1214000.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGCACGACTGGGCCAACACCCTCGCTTCATCTGTTGTACAACGTTCGTCTACTTGGACAACGGTCCGGACTCGACCCTTCTGCGTAGAGAGCTCAAATGGCTCGTGGAGGACGCAATTGAAGAACCTGAAAATCTTGATAACAATGGGGGACGCGTTAGGCTGAGGGTGGAAATTGAGGGGCTTTATATGTTGTGGAAGCAGAGGGTTGAGGAGAGGAGGCCTTTTCAGTATGTTGTCGGGTGCGAGCATTGGAGGGACTTGGTGTTGTGTGTTGAAGAAGTGGGGTTGTGGGCTGATTTGGGGACGGGGAGCGGCGCCATTGCGATTGGGATTGCGAGGGTTTTGGGGAGTGGTGGGAGAGTCATTGCTACGGATTTGAGTCCCGCGGCAATTGGGGTTGCAGCTTTTAATGTGCAGAGATATGGTTTGCAGGATGTAGTGGAGCTAAGGCAAGGATCTTGGTTTGAACCATTAAAGGATGTGGAAGGTAAAGTCTCAGGTTTTGTGAGTAATCCACCGTACATACCAAGTGCCAATATATCAGGGCTGCAAGCTGATGTTGGACGGCATGAACCTAGAGTTGCATTGGACAATGGTATCAATGGCATTGTTGATCTTTTACATCTTTGCAAAGGGACTGCTTTTATGTTGAAACCCGGTGGATTCTTTGCGTTCGAAGTATCTATTTTTGCGTTTGCTAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

237

Amino Acids

26.03

Weight (kDa)

5.46

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 100 - 160 1.9e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 315
AccI GTMKAC 1 cut(s) 51
AccII CGCG 2 cut(s) 156, 389
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 3 cut(s) 315, 387, 389
AclI AACGTT 1 cut(s) 44
AclWI GGATC 1 cut(s) 466
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 2 cut(s) 40, 524
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 4 cut(s) 122, 278, 470, 655
AluBI AGCT 4 cut(s) 94, 407, 448, 557
AluI AGCT 4 cut(s) 94, 407, 448, 557
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 502
AlwI GGATC 1 cut(s) 466
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 2 cut(s) 13, 221
ApeKI GCWGC 2 cut(s) 404, 550
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 13, 65
AsuC2I CCSGG 1 cut(s) 660
AsuII TTCGAA 1 cut(s) 678
AvaII GGWCC 1 cut(s) 65
BaeI ACNNNNGTAYC 2 cut(s) 583, 616
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 96
BauI CACGAG 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 164
BbvI GCAGC 2 cut(s) 416, 537
BceAI ACGGC 1 cut(s) 584
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BcnI CCSGG 1 cut(s) 660
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 321
BisI GCNGC 4 cut(s) 316, 390, 405, 551
BlsI GCNGC 4 cut(s) 317, 391, 406, 552
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 13, 65
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 660
BmrI ACTGGG 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 20
BoxI GACNNNNGTC 1 cut(s) 63
Bpu10I CCTNAGC 2 cut(s) 164, 449
Bpu14I TTCGAA 1 cut(s) 678
BpuMI CCSGG 1 cut(s) 660
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 2 cut(s) 321, 366
BseAI TCCGGA 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 442, 487
BseMI GCAATG 2 cut(s) 321, 366
BseMII CTCAG 2 cut(s) 155, 513
BseNI ACTGG 1 cut(s) 15
BseRI GAGGAG 2 cut(s) 227, 232
BseXI GCAGC 2 cut(s) 416, 537
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 2 cut(s) 156, 389
BshFI GGCC 2 cut(s) 15, 223
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 2 cut(s) 68, 660
BslFI GGGAC 5 cut(s) 165, 272, 319, 369, 652
BsmAI GTCTC 1 cut(s) 502
BsmFI GGGAC 5 cut(s) 165, 272, 319, 369, 652
BsnI GGCC 2 cut(s) 15, 223
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 38
Bsp143I GATC 2 cut(s) 458, 616
BspACI CCGC 3 cut(s) 315, 387, 389
BspANI GGCC 2 cut(s) 15, 223
BspCNI CTCAG 2 cut(s) 156, 512
BspEI TCCGGA 1 cut(s) 67
BspFNI CGCG 2 cut(s) 156, 389
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 1 cut(s) 466
BspT104I TTCGAA 1 cut(s) 678
BspT107I GGYRCC 1 cut(s) 317
BsrBI CCGCTC 1 cut(s) 315
BsrDI GCAATG 2 cut(s) 321, 366
BsrGI TGTACA 1 cut(s) 38
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 458, 616
BssNI GRCGYC 1 cut(s) 318
BssSI CACGAG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 104
Bst4CI ACNGT 2 cut(s) 65, 522
BstACI GRCGYC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 678
BstC8I GCNNGC 2 cut(s) 248, 555
BstDEI CTNAG 3 cut(s) 164, 449, 499
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 442, 487
BstFNI CGCG 2 cut(s) 156, 389
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 2 cut(s) 461, 619
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 2 cut(s) 458, 616
BstMWI GCNNNNNNNGC 2 cut(s) 91, 100
BstPAI GACNNNNGTC 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 658
BstUI CGCG 2 cut(s) 156, 389
BstV1I GCAGC 2 cut(s) 416, 537
BstX2I RGATCY 1 cut(s) 458
BstYI RGATCY 1 cut(s) 458
BsuRI GGCC 2 cut(s) 15, 223
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 442, 487
Cac8I GCNNGC 2 cut(s) 248, 555
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 13, 65
Cfr42I CCGCGG 1 cut(s) 390
CpoI CGGWCCG 1 cut(s) 65
CseI GACGC 2 cut(s) 122, 162
Csp6I GTAC 2 cut(s) 39, 523
CspI CGGWCCG 1 cut(s) 65
CviAII CATG 1 cut(s) 572
CviQI GTAC 2 cut(s) 39, 523
DdeI CTNAG 3 cut(s) 164, 449, 499
DinI GGCGCC 1 cut(s) 319
DpnI GATC 2 cut(s) 460, 618
DpnII GATC 2 cut(s) 458, 616
Ecl136II GAGCTC 1 cut(s) 94
Eco147I AGGCCT 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 96
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
FaeI CATG 1 cut(s) 575
FaiI YATR 8 cut(s) 189, 191, 234, 426, 527, 542, 573, 650
FaqI GGGAC 5 cut(s) 165, 272, 319, 369, 652
FatI CATG 1 cut(s) 571
FauI CCCGC 1 cut(s) 394
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 4 cut(s) 316, 390, 405, 551
FokI GGATG 2 cut(s) 449, 494
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 4 cut(s) 316, 390, 405, 551
FspBI CTAG 1 cut(s) 579
GlaI GCGC 1 cut(s) 319
GluI GCNGC 4 cut(s) 316, 390, 405, 551
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 2 cut(s) 15, 223
HapII CCGG 2 cut(s) 68, 660
HgaI GACGC 2 cut(s) 122, 162
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 1 cut(s) 575
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HinfI GANTC 4 cut(s) 71, 363, 382, 666
HpaII CCGG 2 cut(s) 68, 660
Hpy166II GTNNAC 1 cut(s) 52
Hpy188III TCNNGA 2 cut(s) 68, 137
Hpy8I GTNNAC 1 cut(s) 52
HpyAV CCTTC 2 cut(s) 89, 482
HpyCH4III ACNGT 2 cut(s) 65, 522
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 6 cut(s) 404, 418, 433, 553, 587, 633
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 100
HpyF3I CTNAG 3 cut(s) 164, 449, 499
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 1 cut(s) 575
HspAI GCGC 1 cut(s) 318
KasI GGCGCC 1 cut(s) 317
Kpn2I TCCGGA 1 cut(s) 67
KspI CCGCGG 1 cut(s) 390
Kzo9I GATC 2 cut(s) 458, 616
LmnI GCTCC 2 cut(s) 312, 445
LpnPI CCDG 6 cut(s) 81, 141, 419, 486, 531, 673
Lsp1109I GCAGC 2 cut(s) 416, 537
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 44
MalI GATC 2 cut(s) 460, 618
MbiI CCGCTC 1 cut(s) 315
MboI GATC 2 cut(s) 458, 616
MboII GAAGA 2 cut(s) 134, 290
MfeI CAATTG 2 cut(s) 117, 393
MflI RGATCY 1 cut(s) 458
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 117, 174, 393
MluI ACGCGT 1 cut(s) 154
Mly113I GGCGCC 1 cut(s) 318
MlyI GAGTC 3 cut(s) 65, 372, 391
MmeI TCCRAC 1 cut(s) 544
MroI TCCGGA 1 cut(s) 67
MseI TTAA 2 cut(s) 411, 476
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 2 cut(s) 68, 660
MspR9I CCNGG 1 cut(s) 660
MunI CAATTG 2 cut(s) 117, 393
MvnI CGCG 2 cut(s) 156, 389
MwoI GCNNNNNNNGC 2 cut(s) 91, 100
NarI GGCGCC 1 cut(s) 318
NciI CCSGG 1 cut(s) 660
NdeII GATC 2 cut(s) 458, 616
NlaIII CATG 1 cut(s) 575
NlaIV GGNNCC 1 cut(s) 319
NspV TTCGAA 1 cut(s) 678
PceI AGGCCT 1 cut(s) 223
PfeI GAWTC 1 cut(s) 666
PkrI GCNGC 4 cut(s) 317, 391, 406, 552
PleI GAGTC 3 cut(s) 65, 371, 390
PluTI GGCGCC 1 cut(s) 321
PpsI GAGTC 3 cut(s) 65, 371, 390
PshAI GACNNNNGTC 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 96
Psp1406I AACGTT 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 13, 65
PsuI RGATCY 1 cut(s) 458
RsaI GTAC 2 cut(s) 40, 524
RsaNI GTAC 2 cut(s) 39, 523
Rsr2I CGGWCCG 1 cut(s) 65
RsrII CGGWCCG 1 cut(s) 65
SacI GAGCTC 1 cut(s) 96
SacII CCGCGG 1 cut(s) 390
SaqAI TTAA 2 cut(s) 411, 476
SatI GCNGC 4 cut(s) 316, 390, 405, 551
Sau3AI GATC 2 cut(s) 458, 616
Sau96I GGNCC 2 cut(s) 13, 65
SchI GAGTC 3 cut(s) 65, 372, 391
ScrFI CCNGG 1 cut(s) 660
SduI GDGCHC 1 cut(s) 96
SetI ASST 9 cut(s) 47, 96, 130, 409, 450, 493, 505, 559, 580
SfoI GGCGCC 1 cut(s) 319
Sfr303I CCGCGG 1 cut(s) 390
SfuI TTCGAA 1 cut(s) 678
SgrBI CCGCGG 1 cut(s) 390
SinI GGWCC 1 cut(s) 65
Sse9I AATT 3 cut(s) 117, 174, 393
SseBI AGGCCT 1 cut(s) 223
SsiI CCGC 3 cut(s) 315, 387, 389
SspDI GGCGCC 1 cut(s) 317
SspMI CTAG 1 cut(s) 579
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 658
TaaI ACNGT 2 cut(s) 65, 522
TaiI ACGT 1 cut(s) 47
TaqI TCGA 2 cut(s) 74, 678
TasI AATT 3 cut(s) 117, 174, 393
TatI WGTACW 1 cut(s) 38
TauI GCSGC 2 cut(s) 318, 392
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 2 cut(s) 411, 476
Tru9I TTAA 2 cut(s) 411, 476
TseI GCWGC 2 cut(s) 404, 550
TspDTI ATGAA 2 cut(s) 19, 588
TspGWI ACGGA 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.