Rmu_sc0000847.1_g000007

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000847.1
OG0003404
Physical Location & Seq
Forward (+)
27959 .. 30959
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000847.1_g000007.1.cds

Sequence Viewer

Length: 519 bp
atgcgacctaagcccaagagaaatcgaagccatgcatcgaccccgacctttctcttccgctgtccttctcgctgccactgccgggaaacccaacttccggtcaccacctcacatcaccgccactaccaaaccgtagctcagaggatgacgagcaaaactcgagcgacaacttcaaattccatggcaggactcgagcgtgtgcatgttgatttggagagtttcagagagggtttgtgggcagatttggggactgggagtggggctattgctattgggattgggagggttttggggactggtgtcagagtcattgcaactgatttgagtctcactgcgattttgattgcgggttttaatgtggagagggaggtgccaagtagtaacattgcttttatattcatcagcatatatttgagccctggagaggcagtaattgttgctcaggaagttgtcaatacaatggctgcgaagggatatgatctgggacaagatgcattaatcaaggccaaaaccttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

172

Amino Acids

18.74

Weight (kDa)

9.77

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AciI CCGC 3 cut(s) 58, 118, 347
AcsI RAATTY 1 cut(s) 175
AfiI CCNNNNNNNGG 3 cut(s) 82, 97, 424
AgsI TTSAA 1 cut(s) 174
AjnI CCWGG 1 cut(s) 418
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 332
Ama87I CYCGRG 2 cut(s) 159, 191
AoxI GGCC 1 cut(s) 504
ApeKI GCWGC 2 cut(s) 72, 464
ApoI RAATTY 1 cut(s) 175
AseI ATTAAT 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 94, 107
AvaI CYCGRG 2 cut(s) 159, 191
BanI GGYRCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 59, 451
BciT130I CCWGG 1 cut(s) 420
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 332
BisI GCNGC 2 cut(s) 73, 465
BlsI GCNGC 2 cut(s) 74, 466
Bme1390I CCNGG 2 cut(s) 83, 420
BmeT110I CYCGRG 2 cut(s) 159, 191
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 2 cut(s) 83, 420
BmrI ACTGGG 1 cut(s) 261
BmsI GCATC 2 cut(s) 44, 481
BmuI ACTGGG 1 cut(s) 261
BoxI GACNNNNGTC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 142, 174
BpmI CTGGAG 1 cut(s) 441
Bpu10I CCTNAGC 2 cut(s) 9, 441
BpuMI CCSGG 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 180, 418
BsaWI WCCGGW 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 97, 424
Bse1I ACTGG 2 cut(s) 256, 301
Bse3DI GCAATG 2 cut(s) 309, 384
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 180, 418
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 3 cut(s) 82, 97, 424
BseMI GCAATG 2 cut(s) 309, 384
BseMII CTCAG 2 cut(s) 152, 455
BseNI ACTGG 2 cut(s) 256, 301
BseXI GCAGC 2 cut(s) 59, 451
BshFI GGCC 1 cut(s) 506
BshNI GGYRCC 1 cut(s) 370
BsiHKCI CYCGRG 2 cut(s) 159, 191
BsiSI CCGG 2 cut(s) 82, 98
BslFI GGGAC 3 cut(s) 262, 307, 498
BslI CCNNNNNNNGG 3 cut(s) 82, 97, 424
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 3 cut(s) 262, 307, 498
BsnI GGCC 1 cut(s) 506
BsoBI CYCGRG 2 cut(s) 159, 191
Bsp1286I GDGCHC 1 cut(s) 419
Bsp143I GATC 1 cut(s) 478
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 3 cut(s) 58, 118, 347
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 2 cut(s) 151, 454
BspLI GGNNCC 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 370
BsrDI GCAATG 2 cut(s) 309, 384
BsrI ACTGG 2 cut(s) 256, 301
BssECI CCNNGG 2 cut(s) 180, 418
BssMI GATC 1 cut(s) 478
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 59
BstDEI CTNAG 3 cut(s) 9, 138, 441
BstDSI CCRYGG 1 cut(s) 180
BstEII GGTNACC 1 cut(s) 100
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 10, 78
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 206
BstPAI GACNNNNGTC 1 cut(s) 299
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 81, 418
BstV1I GCAGC 2 cut(s) 59, 451
BsuRI GGCC 1 cut(s) 506
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 1 cut(s) 150
BtsI GCAGTG 2 cut(s) 76, 330
BtsIMutI CAGTG 2 cut(s) 76, 330
CviAII CATG 3 cut(s) 32, 181, 203
CviJI RGCY 7 cut(s) 13, 30, 137, 263, 417, 464, 506
CviKI_1 RGCY 7 cut(s) 13, 30, 137, 263, 417, 464, 506
DdeI CTNAG 3 cut(s) 9, 138, 441
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 419
Eco88I CYCGRG 2 cut(s) 159, 191
Eco91I GGTNACC 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 2 cut(s) 37, 496
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 3 cut(s) 35, 184, 206
FaiI YATR 7 cut(s) 33, 182, 204, 395, 407, 409, 477
FaqI GGGAC 3 cut(s) 262, 307, 498
FatI CATG 3 cut(s) 31, 180, 202
FauI CCCGC 1 cut(s) 340
Fnu4HI GCNGC 2 cut(s) 73, 465
FokI GGATG 1 cut(s) 157
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 2 cut(s) 73, 465
GluI GCNGC 2 cut(s) 73, 465
GsuI CTGGAG 1 cut(s) 441
HaeIII GGCC 1 cut(s) 506
HapII CCGG 2 cut(s) 82, 98
Hin1II CATG 3 cut(s) 35, 184, 206
HinfI GANTC 3 cut(s) 189, 306, 325
HpaII CCGG 2 cut(s) 82, 98
HphI GGTGA 2 cut(s) 94, 107
Hpy188I TCNGA 4 cut(s) 141, 224, 305, 518
Hpy188III TCNNGA 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 75, 463
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4V TGCA 4 cut(s) 35, 202, 314, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 78
HpyF3I CTNAG 3 cut(s) 9, 138, 441
Hsp92II CATG 3 cut(s) 35, 184, 206
Kzo9I GATC 1 cut(s) 478
LpnPI CCDG 9 cut(s) 95, 111, 171, 237, 282, 405, 428, 432, 467
Lsp1109I GCAGC 2 cut(s) 59, 451
LweI GCATC 2 cut(s) 44, 481
MaeIII GTNAC 2 cut(s) 100, 380
MalI GATC 1 cut(s) 480
MboI GATC 1 cut(s) 478
MboII GAAGA 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 2 cut(s) 175, 432
MlyI GAGTC 3 cut(s) 183, 315, 334
MnlI CCTC 7 cut(s) 118, 135, 220, 276, 357, 361, 418
Mph1103I ATGCAT 2 cut(s) 37, 496
MseI TTAA 2 cut(s) 354, 497
MspA1I CMGCKG 1 cut(s) 60
MspI CCGG 2 cut(s) 82, 98
MspR9I CCNGG 2 cut(s) 83, 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 2 cut(s) 10, 78
NciI CCSGG 1 cut(s) 83
NcoI CCATGG 1 cut(s) 180
NdeII GATC 1 cut(s) 478
NlaIII CATG 3 cut(s) 35, 184, 206
NlaIV GGNNCC 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 100
NsiI ATGCAT 2 cut(s) 37, 496
NspI RCATGY 1 cut(s) 206
PaeR7I CTCGAG 2 cut(s) 159, 191
PkrI GCNGC 2 cut(s) 74, 466
PleI GAGTC 3 cut(s) 183, 314, 333
PpsI GAGTC 3 cut(s) 183, 314, 333
PshAI GACNNNNGTC 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 418
PspEI GGTNACC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 372
PspXI VCTCGAGB 2 cut(s) 159, 191
SaqAI TTAA 2 cut(s) 354, 497
SatI GCNGC 2 cut(s) 73, 465
Sau3AI GATC 1 cut(s) 478
SchI GAGTC 3 cut(s) 183, 315, 334
ScrFI CCNGG 2 cut(s) 83, 420
SduI GDGCHC 1 cut(s) 419
SetI ASST 6 cut(s) 10, 50, 110, 139, 372, 515
SfaNI GCATC 2 cut(s) 44, 481
Sfr274I CTCGAG 2 cut(s) 159, 191
SlaI CTCGAG 2 cut(s) 159, 191
SmlI CTYRAG 2 cut(s) 159, 191
SmoI CTYRAG 2 cut(s) 159, 191
Sse9I AATT 2 cut(s) 175, 432
SsiI CCGC 3 cut(s) 58, 118, 347
StyD4I CCNGG 2 cut(s) 81, 418
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 133
TaqI TCGA 4 cut(s) 25, 38, 160, 192
TasI AATT 2 cut(s) 175, 432
Tru1I TTAA 2 cut(s) 354, 497
Tru9I TTAA 2 cut(s) 354, 497
TscAI CASTG 2 cut(s) 83, 337
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 2 cut(s) 72, 464
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 388
TspRI CASTG 2 cut(s) 83, 337
VspI ATTAAT 1 cut(s) 497
XapI RAATTY 1 cut(s) 175
XceI RCATGY 1 cut(s) 206
XhoI CTCGAG 2 cut(s) 159, 191
Zsp2I ATGCAT 2 cut(s) 37, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.