Rw5G017360

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003404
Physical Location & Seq
Reverse (-)
22114375 .. 22116607
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017360.1

Sequence Viewer

Length: 894 bp
ATGGAGTTGATTGTTTTTGAAATTCTCTATCGATTGTTTGGGTCGACCTTCGCCGACTCAGACAACGGCCCAGACTCGACCCTTTTGCTCAGAGAGCTCAACTGGCTCGTAGACGACGCAGTTCAACAAGGAATCGAAAATGGTCAAAGAAAGATTAGATTGAGAGTGAGTCTGGAGGAGCTGTACTTGTTGTGGAAGCAGAGGATTGAAGAGAGGAAGCCCTTTCAGTACATTGTTGGGTGTGAGCATTGGAGGGAGTTGGTGCTGTGCGTTCAAGAAGGGGTTTTGATTCCGAGGCCGGAGACTGAGCTCATTGTGGATTTGGTGGGTGATGTGGTGAAGGGTGAGGAGGGTTTAAGAGAGGGTTTGTGGGCAGATTTGGGGACTGGGAGTGGGGCTATTGCTATTGGGATTGCGAGGGTTTTGGGGACTGGTGGTGGCAGAGTCATTGCAACTGATTTGAGTCCCACTGCGATTTCGGTTGCGGGGTTTAATGTGGAGAGGTATGGTTTGCAGGATGTAGTGGAGCTAAGGCAAGGATCTTGGTTTGACCCATTAAAGGATGTGGAAGGTAAACTTGCAGGGTTCGTAAGTAATCCACCGTACATACCAAGTGACAATATCTCGGGGCTACAAGCTGAAGTTGGAAGACATGAACCCAGAGTTGCACTGGATGGCGGTGTTGATGGCATGGATGATCTCTTACATCTTTGCAAAGGGGCTGCTTCCATTTTGAAACCTGGTGGATTTTTTGCTTTTGAGACAAATGGTGAGAAGCAGTGCAAGTATCTTGTGGAGTACATGGAAAATGAGGCTAGAGGCAGCTTTTGTAATTTGAGTATAATTCCTGATTTTGCCGGTATTCATAGATTTGTAACCGGATTCCATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

297

Amino Acids

32.79

Weight (kDa)

4.83

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 126 - 187 4.4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 44, 111
AciI CCGC 2 cut(s) 485, 678
AclWI GGATC 1 cut(s) 547
AcsI RAATTY 1 cut(s) 21
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 4 cut(s) 185, 230, 605, 800
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 5 cut(s) 20, 125, 209, 275, 736
AjnI CCWGG 1 cut(s) 739
AluBI AGCT 6 cut(s) 97, 181, 310, 529, 638, 825
AluI AGCT 6 cut(s) 97, 181, 310, 529, 638, 825
Alw21I GWGCWC 2 cut(s) 99, 312
Alw26I GTCTC 2 cut(s) 296, 755
AlwI GGATC 1 cut(s) 547
Ama87I CYCGRG 1 cut(s) 625
AoxI GGCC 2 cut(s) 67, 296
ApeKI GCWGC 2 cut(s) 722, 822
ApoI RAATTY 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 4 cut(s) 341, 349, 356, 782
AvaI CYCGRG 1 cut(s) 625
BanII GRGCYC 2 cut(s) 99, 312
BbsI GAAGAC 1 cut(s) 655
Bbv12I GWGCWC 2 cut(s) 99, 312
BbvI GCAGC 2 cut(s) 709, 834
BccI CCATC 2 cut(s) 668, 680
BceAI ACGGC 1 cut(s) 82
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 296, 755
BfaI CTAG 1 cut(s) 816
BisI GCNGC 2 cut(s) 723, 823
BlsI GCNGC 2 cut(s) 724, 824
Bme1390I CCNGG 1 cut(s) 741
BmeT110I CYCGRG 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 68
BmrFI CCNGG 1 cut(s) 741
BmrI ACTGGG 1 cut(s) 396
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 655
BpmI CTGGAG 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 530
Bsa29I ATCGAT 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 293
BsaWI WCCGGW 1 cut(s) 878
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 4 cut(s) 107, 391, 436, 675
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 1 cut(s) 741
BseCI ATCGAT 1 cut(s) 31
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 4 cut(s) 523, 568, 679, 700
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 3 cut(s) 72, 103, 297
BseNI ACTGG 4 cut(s) 107, 391, 436, 675
BseRI GAGGAG 2 cut(s) 191, 362
BseXI GCAGC 2 cut(s) 709, 834
BshFI GGCC 2 cut(s) 69, 298
BshVI ATCGAT 1 cut(s) 31
BsiHKAI GWGCWC 2 cut(s) 99, 312
BsiHKCI CYCGRG 1 cut(s) 625
BsiSI CCGG 3 cut(s) 299, 858, 879
BslFI GGGAC 3 cut(s) 397, 442, 450
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 296, 755
BsmFI GGGAC 3 cut(s) 397, 442, 450
BsnI GGCC 2 cut(s) 69, 298
BsoBI CYCGRG 1 cut(s) 625
Bsp1286I GDGCHC 2 cut(s) 99, 312
Bsp143I GATC 2 cut(s) 539, 697
BspACI CCGC 2 cut(s) 485, 678
BspANI GGCC 2 cut(s) 69, 298
BspCNI CTCAG 3 cut(s) 71, 102, 298
BspDI ATCGAT 1 cut(s) 31
BspPI GGATC 1 cut(s) 547
BsrDI GCAATG 1 cut(s) 447
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 4 cut(s) 107, 391, 436, 675
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 2 cut(s) 539, 697
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 1 cut(s) 603
Bst6I CTCTTC 1 cut(s) 204
BstDEI CTNAG 4 cut(s) 58, 89, 306, 530
BstF5I GGATG 4 cut(s) 523, 568, 679, 700
BstKTI GATC 2 cut(s) 542, 700
BstMAI GTCTC 2 cut(s) 296, 755
BstMBI GATC 2 cut(s) 539, 697
BstMWI GCNNNNNNNGC 2 cut(s) 94, 103
BstNI CCWGG 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 739
BstV1I GCAGC 2 cut(s) 709, 834
BstV2I GAAGAC 1 cut(s) 655
BstX2I RGATCY 1 cut(s) 539
BstYI RGATCY 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 31
BsuRI GGCC 2 cut(s) 69, 298
BsuTUI ATCGAT 1 cut(s) 31
BtsCI GGATG 4 cut(s) 523, 568, 679, 700
BtsI GCAGTG 2 cut(s) 468, 785
BtsIMutI CAGTG 3 cut(s) 468, 668, 785
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 1 cut(s) 68
ClaI ATCGAT 1 cut(s) 31
CseI GACGC 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 739
Csp6I GTAC 4 cut(s) 184, 229, 604, 799
CviAII CATG 3 cut(s) 653, 691, 802
CviQI GTAC 4 cut(s) 184, 229, 604, 799
DdeI CTNAG 4 cut(s) 58, 89, 306, 530
DpnI GATC 2 cut(s) 541, 699
DpnII GATC 2 cut(s) 539, 697
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Ecl136II GAGCTC 2 cut(s) 97, 310
Eco24I GRGCYC 2 cut(s) 99, 312
Eco53kI GAGCTC 2 cut(s) 97, 310
Eco57I CTGAAG 1 cut(s) 660
Eco88I CYCGRG 1 cut(s) 625
EcoICRI GAGCTC 2 cut(s) 97, 310
EcoRII CCWGG 1 cut(s) 739
EcoT38I GRGCYC 2 cut(s) 99, 312
FaeI CATG 3 cut(s) 656, 694, 805
FaiI YATR 8 cut(s) 507, 608, 654, 692, 803, 842, 867, 888
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FaqI GGGAC 3 cut(s) 397, 442, 450
FatI CATG 3 cut(s) 652, 690, 801
FauI CCCGC 1 cut(s) 478
FblI GTMKAC 2 cut(s) 44, 111
Fnu4HI GCNGC 2 cut(s) 723, 823
FokI GGATG 4 cut(s) 530, 575, 686, 707
FriOI GRGCYC 2 cut(s) 99, 312
Fsp4HI GCNGC 2 cut(s) 723, 823
FspBI CTAG 1 cut(s) 816
GluI GCNGC 2 cut(s) 723, 823
GsuI CTGGAG 1 cut(s) 194
HaeIII GGCC 2 cut(s) 69, 298
HapII CCGG 3 cut(s) 299, 858, 879
HgaI GACGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 656, 694, 805
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 8 cut(s) 56, 74, 132, 169, 289, 444, 463, 882
HpaII CCGG 3 cut(s) 299, 858, 879
HphI GGTGA 4 cut(s) 341, 349, 356, 782
Hpy166II GTNNAC 3 cut(s) 45, 112, 575
Hpy188I TCNGA 3 cut(s) 61, 92, 294
Hpy188III TCNNGA 3 cut(s) 173, 275, 848
Hpy8I GTNNAC 3 cut(s) 45, 112, 575
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 4 cut(s) 58, 272, 334, 563
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4V TGCA 6 cut(s) 452, 514, 581, 668, 714, 783
HpyF10VI GCNNNNNNNGC 2 cut(s) 94, 103
HpyF3I CTNAG 4 cut(s) 58, 89, 306, 530
Hsp92II CATG 3 cut(s) 656, 694, 805
Kzo9I GATC 2 cut(s) 539, 697
LmnI GCTCC 2 cut(s) 178, 526
Lsp1109I GCAGC 2 cut(s) 709, 834
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 1 cut(s) 816
MaeIII GTNAC 2 cut(s) 614, 874
MalI GATC 2 cut(s) 541, 699
MboI GATC 2 cut(s) 539, 697
MboII GAAGA 2 cut(s) 221, 660
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 2 cut(s) 99, 312
MluCI AATT 3 cut(s) 21, 832, 843
MlyI GAGTC 5 cut(s) 50, 68, 178, 453, 472
MmeI TCCRAC 1 cut(s) 625
MseI TTAA 3 cut(s) 356, 492, 557
MspI CCGG 3 cut(s) 299, 858, 879
MspR9I CCNGG 1 cut(s) 741
MvaI CCWGG 1 cut(s) 741
MwoI GCNNNNNNNGC 2 cut(s) 94, 103
NdeII GATC 2 cut(s) 539, 697
NlaIII CATG 3 cut(s) 656, 694, 805
NmuCI GTSAC 1 cut(s) 614
PfeI GAWTC 3 cut(s) 132, 289, 882
PkrI GCNGC 2 cut(s) 724, 824
PleI GAGTC 5 cut(s) 50, 68, 177, 452, 471
PpsI GAGTC 5 cut(s) 50, 68, 177, 452, 471
Psp124BI GAGCTC 2 cut(s) 99, 312
Psp6I CCWGG 1 cut(s) 739
PspGI CCWGG 1 cut(s) 739
PspPI GGNCC 1 cut(s) 68
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 4 cut(s) 185, 230, 605, 800
RsaNI GTAC 4 cut(s) 184, 229, 604, 799
SacI GAGCTC 2 cut(s) 99, 312
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 3 cut(s) 356, 492, 557
SatI GCNGC 2 cut(s) 723, 823
Sau3AI GATC 2 cut(s) 539, 697
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 5 cut(s) 50, 68, 178, 453, 472
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 2 cut(s) 99, 312
SexAI ACCWGGT 1 cut(s) 739
Sse9I AATT 3 cut(s) 21, 832, 843
SsiI CCGC 2 cut(s) 485, 678
SspMI CTAG 1 cut(s) 816
SstI GAGCTC 2 cut(s) 99, 312
StyD4I CCNGG 1 cut(s) 739
TaaI ACNGT 1 cut(s) 603
TaqI TCGA 4 cut(s) 31, 44, 77, 135
TasI AATT 3 cut(s) 21, 832, 843
TatI WGTACW 3 cut(s) 183, 228, 798
TfiI GAWTC 3 cut(s) 132, 289, 882
Tru1I TTAA 3 cut(s) 356, 492, 557
Tru9I TTAA 3 cut(s) 356, 492, 557
TscAI CASTG 3 cut(s) 475, 675, 785
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 2 cut(s) 722, 822
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 2 cut(s) 669, 854
TspRI CASTG 3 cut(s) 475, 675, 785
XapI RAATTY 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XmiI GTMKAC 2 cut(s) 44, 111
XspI CTAG 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.