Rh5AG190600

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003404
Physical Location & Seq
Reverse (-)
21953407 .. 21957815
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG190600.1

Sequence Viewer

Length: 1080 bp
ATGAAGCTAAGCCTCACTCCAAAACTCTCAACCTTCCTTAAACCCTCCCTTGCTTCTACTCCCCTCCGTCGCTCTCTTTGCTCTTCTTCACCATCACCACCACCACCACCACCCATTTCTCTAAAACCCCAAACCCCTCTCTTTCTGAGACCACCCATTCACTCAGCCACCTCCTCAGACCTCCAGCAATGGCAGGACTGGGCCAAAACCCTCGCTTCCTCAGTTGGCTCTACCTTCGCCGACTCCGACAACGGCCCCGACTCGACCCTTTTGCTCAGAGAGCTCAACTGGCTCGTAGACGACGCAGTTCAACAAGGAATCGAAAATGGTCAAAGAAAAATTAGATTGAGAGTGAGTCTGGAGGAGCTGTACTTGTTGTGGAAGCAGAGGATTGAAGAGAGGAAGCCCTTTCAGTACATTGTTGGGTGTGAGCATTGGAGGGAGCTGGTGCTGTGTGTTCAAGAAGGGGTTTTGATTCCGAGGCCGGAGACTGAGCTCATTGTGGATTTGGTGGGTGATGTGGTGAAGGGTGAGGAGGGTTTAAGAGAGGGTTTGTGGGCAGATTTGGGGACTGGGAGTGGGGCTATTGCTATTGGGATTGCGAGGGTTTTGGGGACTGGTGGTGGCAGAGTCATTGCAACTGATTTGAGTCCCACTGCGATTTCGGTTGCGGGGTTTAATGTGGAGAGGTATGGTTTGCAGGATGTAGTGGAGCTAAGGCAAGGATCTTGGTTTGACCCATTAAAGGATGTGGAAGGTAAACTTGCGGGGTTCGTAAGTAATCCACCGTACATACCAAGTGACAATATCTCGGGGCTACAAGCTGAAGTTGGAAGACATGAACCCAGAGTTGCACTGGATGGCGGTGTTGATGGCATGGATGATCTCTTACATCTTTGCAAAGGGGCTGCTTCCATTTTGAAACCTGGTGGATTTTTTGCTTTTGAGACAAATGGTGAGAAGCAGTGCAAGTATCTTGTGGAGTACATGGAAAATGAGGCTAGAGGCAGCTTTTGTAATTTGAGTATAATTCCTGATTTTGCGGGTATTCATAGATTTGTTACCGGATTCCATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

359

Amino Acids

39.26

Weight (kDa)

5.4

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 188 - 249 6.2e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 297
AciI CCGC 4 cut(s) 671, 767, 864, 1043
AclWI GGATC 1 cut(s) 733
AcuI CTGAAG 1 cut(s) 846
AfaI GTAC 4 cut(s) 371, 416, 791, 986
AfiI CCNNNNNNNGG 1 cut(s) 745
AgsI TTSAA 4 cut(s) 311, 395, 461, 922
AjnI CCWGG 1 cut(s) 925
AluBI AGCT 8 cut(s) 7, 283, 367, 445, 496, 715, 824, 1011
AluI AGCT 8 cut(s) 7, 283, 367, 445, 496, 715, 824, 1011
Alw21I GWGCWC 2 cut(s) 285, 498
Alw26I GTCTC 3 cut(s) 142, 482, 941
AlwI GGATC 1 cut(s) 733
Ama87I CYCGRG 1 cut(s) 811
AoxI GGCC 3 cut(s) 201, 253, 482
ApeKI GCWGC 2 cut(s) 908, 1008
AspS9I GGNCC 2 cut(s) 201, 254
AsuHPI GGTGA 6 cut(s) 81, 87, 527, 535, 542, 968
AvaI CYCGRG 1 cut(s) 811
BanII GRGCYC 2 cut(s) 285, 498
BbsI GAAGAC 1 cut(s) 841
Bbv12I GWGCWC 2 cut(s) 285, 498
BbvI GCAGC 2 cut(s) 895, 1020
BccI CCATC 3 cut(s) 100, 854, 866
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 1 cut(s) 927
BcoDI GTCTC 3 cut(s) 142, 482, 941
BfaI CTAG 1 cut(s) 1002
BisI GCNGC 2 cut(s) 909, 1009
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 2 cut(s) 910, 1010
Bme1390I CCNGG 1 cut(s) 927
BmeT110I CYCGRG 1 cut(s) 811
BmgT120I GGNCC 2 cut(s) 201, 254
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 927
BmrI ACTGGG 2 cut(s) 208, 582
BmuI ACTGGG 2 cut(s) 208, 582
BpiI GAAGAC 1 cut(s) 841
BpmI CTGGAG 2 cut(s) 167, 380
Bpu10I CCTNAGC 1 cut(s) 716
Bpu1102I GCTNAGC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 479
BsaWI WCCGGW 1 cut(s) 1064
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 745
Bse1I ACTGG 5 cut(s) 203, 293, 577, 622, 861
Bse3DI GCAATG 2 cut(s) 194, 633
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 1 cut(s) 479
BseGI GGATG 4 cut(s) 709, 754, 865, 886
BseLI CCNNNNNNNGG 1 cut(s) 745
BseMI GCAATG 2 cut(s) 194, 633
BseMII CTCAG 6 cut(s) 137, 177, 189, 234, 289, 483
BseNI ACTGG 5 cut(s) 203, 293, 577, 622, 861
BseRI GAGGAG 3 cut(s) 163, 377, 548
BseXI GCAGC 2 cut(s) 895, 1020
BshFI GGCC 3 cut(s) 203, 255, 484
BsiHKAI GWGCWC 2 cut(s) 285, 498
BsiHKCI CYCGRG 1 cut(s) 811
BsiSI CCGG 2 cut(s) 485, 1065
BslFI GGGAC 3 cut(s) 583, 628, 636
BslI CCNNNNNNNGG 1 cut(s) 745
BsmAI GTCTC 3 cut(s) 142, 482, 941
BsmFI GGGAC 3 cut(s) 583, 628, 636
BsnI GGCC 3 cut(s) 203, 255, 484
Bso31I GGTCTC 1 cut(s) 142
BsoBI CYCGRG 1 cut(s) 811
Bsp1286I GDGCHC 2 cut(s) 285, 498
Bsp143I GATC 2 cut(s) 725, 883
Bsp1720I GCTNAGC 1 cut(s) 8
BspACI CCGC 4 cut(s) 671, 767, 864, 1043
BspANI GGCC 3 cut(s) 203, 255, 484
BspCNI CTCAG 6 cut(s) 138, 176, 188, 233, 288, 484
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 733
BspQI GCTCTTC 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 142
BsrDI GCAATG 2 cut(s) 194, 633
BsrI ACTGG 5 cut(s) 203, 293, 577, 622, 861
BssECI CCNNGG 1 cut(s) 479
BssMI GATC 2 cut(s) 725, 883
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 1 cut(s) 789
Bst6I CTCTTC 2 cut(s) 88, 390
BstDEI CTNAG 8 cut(s) 8, 146, 163, 175, 220, 275, 492, 716
BstF5I GGATG 4 cut(s) 709, 754, 865, 886
BstKTI GATC 2 cut(s) 728, 886
BstMAI GTCTC 3 cut(s) 142, 482, 941
BstMBI GATC 2 cut(s) 725, 883
BstMWI GCNNNNNNNGC 3 cut(s) 78, 280, 289
BstNI CCWGG 1 cut(s) 927
BstSCI CCNGG 1 cut(s) 925
BstV1I GCAGC 2 cut(s) 895, 1020
BstV2I GAAGAC 1 cut(s) 841
BstX2I RGATCY 1 cut(s) 725
BstYI RGATCY 1 cut(s) 725
BsuRI GGCC 3 cut(s) 203, 255, 484
BtsCI GGATG 4 cut(s) 709, 754, 865, 886
BtsI GCAGTG 2 cut(s) 654, 971
BtsIMutI CAGTG 3 cut(s) 654, 854, 971
Cfr13I GGNCC 2 cut(s) 201, 254
CseI GACGC 1 cut(s) 311
CsiI ACCWGGT 1 cut(s) 925
Csp6I GTAC 4 cut(s) 370, 415, 790, 985
CviAII CATG 3 cut(s) 839, 877, 988
CviQI GTAC 4 cut(s) 370, 415, 790, 985
DdeI CTNAG 8 cut(s) 8, 146, 163, 175, 220, 275, 492, 716
DpnI GATC 2 cut(s) 727, 885
DpnII GATC 2 cut(s) 725, 883
Eam1104I CTCTTC 2 cut(s) 88, 390
EarI CTCTTC 2 cut(s) 88, 390
Ecl136II GAGCTC 2 cut(s) 283, 496
Eco24I GRGCYC 2 cut(s) 285, 498
Eco31I GGTCTC 1 cut(s) 142
Eco53kI GAGCTC 2 cut(s) 283, 496
Eco57I CTGAAG 1 cut(s) 846
Eco88I CYCGRG 1 cut(s) 811
EcoICRI GAGCTC 2 cut(s) 283, 496
EcoRII CCWGG 1 cut(s) 925
EcoT38I GRGCYC 2 cut(s) 285, 498
FaeI CATG 3 cut(s) 842, 880, 991
FaiI YATR 8 cut(s) 693, 794, 840, 878, 989, 1028, 1053, 1074
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FaqI GGGAC 3 cut(s) 583, 628, 636
FatI CATG 3 cut(s) 838, 876, 987
FauI CCCGC 3 cut(s) 664, 760, 1036
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 909, 1009
FokI GGATG 4 cut(s) 716, 761, 872, 893
FriOI GRGCYC 2 cut(s) 285, 498
Fsp4HI GCNGC 2 cut(s) 909, 1009
FspBI CTAG 1 cut(s) 1002
GluI GCNGC 2 cut(s) 909, 1009
GsuI CTGGAG 2 cut(s) 167, 380
HaeIII GGCC 3 cut(s) 203, 255, 484
HapII CCGG 2 cut(s) 485, 1065
HgaI GACGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 842, 880, 991
HinfI GANTC 8 cut(s) 242, 260, 318, 355, 475, 630, 649, 1068
HpaII CCGG 2 cut(s) 485, 1065
HphI GGTGA 6 cut(s) 81, 87, 527, 535, 542, 968
Hpy166II GTNNAC 2 cut(s) 298, 761
Hpy188I TCNGA 5 cut(s) 147, 178, 247, 278, 480
Hpy188III TCNNGA 3 cut(s) 359, 461, 1034
Hpy8I GTNNAC 2 cut(s) 298, 761
Hpy99I CGWCG 2 cut(s) 72, 305
HpyAV CCTTC 5 cut(s) 43, 244, 458, 520, 749
HpyCH4III ACNGT 1 cut(s) 789
HpyCH4V TGCA 5 cut(s) 638, 700, 854, 900, 969
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 280, 289
HpyF3I CTNAG 8 cut(s) 8, 146, 163, 175, 220, 275, 492, 716
Hsp92II CATG 3 cut(s) 842, 880, 991
Kzo9I GATC 2 cut(s) 725, 883
LguI GCTCTTC 1 cut(s) 88
LmnI GCTCC 3 cut(s) 364, 442, 712
Lsp1109I GCAGC 2 cut(s) 895, 1020
MabI ACCWGGT 1 cut(s) 925
MaeI CTAG 1 cut(s) 1002
MaeIII GTNAC 2 cut(s) 800, 1060
MalI GATC 2 cut(s) 727, 885
MboI GATC 2 cut(s) 725, 883
MboII GAAGA 4 cut(s) 75, 78, 407, 846
MflI RGATCY 1 cut(s) 725
MhlI GDGCHC 2 cut(s) 285, 498
MluCI AATT 3 cut(s) 339, 1018, 1029
MlyI GAGTC 5 cut(s) 236, 254, 364, 639, 658
MmeI TCCRAC 2 cut(s) 270, 811
MseI TTAA 4 cut(s) 39, 542, 678, 743
MspI CCGG 2 cut(s) 485, 1065
MspR9I CCNGG 1 cut(s) 927
MvaI CCWGG 1 cut(s) 927
MwoI GCNNNNNNNGC 3 cut(s) 78, 280, 289
NdeII GATC 2 cut(s) 725, 883
NlaIII CATG 3 cut(s) 842, 880, 991
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 800
PciSI GCTCTTC 1 cut(s) 88
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PfeI GAWTC 3 cut(s) 318, 475, 1068
PkrI GCNGC 2 cut(s) 910, 1010
PleI GAGTC 5 cut(s) 236, 254, 363, 638, 657
PpsI GAGTC 5 cut(s) 236, 254, 363, 638, 657
Psp124BI GAGCTC 2 cut(s) 285, 498
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 2 cut(s) 201, 254
PsuI RGATCY 1 cut(s) 725
RsaI GTAC 4 cut(s) 371, 416, 791, 986
RsaNI GTAC 4 cut(s) 370, 415, 790, 985
SacI GAGCTC 2 cut(s) 285, 498
SapI GCTCTTC 1 cut(s) 88
SaqAI TTAA 4 cut(s) 39, 542, 678, 743
SatI GCNGC 2 cut(s) 909, 1009
Sau3AI GATC 2 cut(s) 725, 883
Sau96I GGNCC 2 cut(s) 201, 254
SchI GAGTC 5 cut(s) 236, 254, 364, 639, 658
ScrFI CCNGG 1 cut(s) 927
SduI GDGCHC 2 cut(s) 285, 498
SexAI ACCWGGT 1 cut(s) 925
Sse9I AATT 3 cut(s) 339, 1018, 1029
SsiI CCGC 4 cut(s) 671, 767, 864, 1043
SspMI CTAG 1 cut(s) 1002
SstI GAGCTC 2 cut(s) 285, 498
StyD4I CCNGG 1 cut(s) 925
TaaI ACNGT 1 cut(s) 789
TaqI TCGA 2 cut(s) 263, 321
TasI AATT 3 cut(s) 339, 1018, 1029
TatI WGTACW 3 cut(s) 369, 414, 984
TfiI GAWTC 3 cut(s) 318, 475, 1068
Tru1I TTAA 4 cut(s) 39, 542, 678, 743
Tru9I TTAA 4 cut(s) 39, 542, 678, 743
TscAI CASTG 3 cut(s) 661, 861, 971
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 2 cut(s) 908, 1008
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 3 cut(s) 17, 855, 1040
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 3 cut(s) 661, 861, 971
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 1 cut(s) 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.