RLG00000032986

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003404
Physical Location & Seq
Reverse (-)
21757160 .. 21759095
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032986

Sequence Viewer

Length: 1077 bp
ATGAAGCTAAGCCTCACTCCAAAACTCTCAACCTTCCTTAAACCCTCCCTTGCTTCTACTCCTCTCCGTCGCTCTATTTGCTCTTCATCACCACCACCACCACCATCACCCATTTCTCTAAAACCCCAAACCCCTCTCTTTCTGAGACCACCCATTCACTCAGCCACCTCCTCAGACCTCCAGCAATGGCAGGACTGGGCCAAAACCCTCGCTTCCTCAGTTGGGTCGACCTTCGCCGACTCAGACAACGGCCCGGACTCGACCCTTTTGCTCAGAGAGCTCAACTGGCTCGTAGACGACGCAGTTCAACAAGGAATCGAAAATGGTCAAAGGAAAATTAGATTGAGAGTGAGTCTGGAGGAGCTGTACTTGTTGTGGAAGCAGAGGATTGAAGAGAGGAGACCCTTTCAGTACATTGTTGGGTGTGAGCATTGGAGGGAGTTGGTGCTGTGTGTTCAAGAAGGGGTTTTGATTCCGAGGCCGGAGACTGAGCTCATTGTGGATTTGGTGGGTGATGTGGTGAAGGGTGAGGAGGGTTTAAGAGAGGGTTTGTGGGCTGATTTGGGGACTGGGAGTGGGGCTATTGCTATTGGGATTGCGAGGGTTTTGGGGACTGGTGGTGGCACAGTCATTGCAACTGATTTGAGTCCCACTGCGATTTCGGTTGCGGGGTTTAATGTGGAGAGGTATGGTTTACAGGATGTAGTGGAGCTAAGGCAAGGATCTTGGTTTGACCCGTTAAAGGATGTGGAAGGTAAACTTGCAGGGTTCGTAAGTAATCCACCGTACATACCAAGTGACAATATCTCGGGGCTACAAGCTGAAGTTGGAAGACATGAACCCAGAGTTGCACTGGATGGCGGTGTTGATGGCATGGATGATCTCTTACATCTTTGCAAAGGGGCTGCTTCCATTTTGAAACCTGGTGGATTTTTTGCTTTTGAGACAAATGGTGAGAAGCAGTGCAAGTATCTTGTGGAGTACATGGAAAATGAGGCTAGAGGCAGCTTCTGTAATTTGAGTATAATTCCTGATTTTGCGGGTATTCATAGATTTGTTACCGGATTCCGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

359

Amino Acids

39.15

Weight (kDa)

5.32

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 187 - 248 5.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 227, 294
AciI CCGC 3 cut(s) 668, 861, 1040
AclWI GGATC 1 cut(s) 730
AcuI CTGAAG 1 cut(s) 843
AfaI GTAC 4 cut(s) 368, 413, 788, 983
AfiI CCNNNNNNNGG 2 cut(s) 222, 742
AgsI TTSAA 4 cut(s) 308, 392, 458, 919
AjnI CCWGG 1 cut(s) 922
AluBI AGCT 7 cut(s) 7, 280, 364, 493, 712, 821, 1008
AluI AGCT 7 cut(s) 7, 280, 364, 493, 712, 821, 1008
Alw21I GWGCWC 2 cut(s) 282, 495
Alw26I GTCTC 4 cut(s) 139, 394, 479, 938
AlwI GGATC 1 cut(s) 730
AlwNI CAGNNNCTG 1 cut(s) 1011
Ama87I CYCGRG 1 cut(s) 808
AoxI GGCC 3 cut(s) 198, 250, 479
ApeKI GCWGC 2 cut(s) 905, 1005
AspS9I GGNCC 2 cut(s) 198, 251
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 6 cut(s) 81, 99, 524, 532, 539, 965
AvaI CYCGRG 1 cut(s) 808
BanII GRGCYC 2 cut(s) 282, 495
BbsI GAAGAC 1 cut(s) 838
Bbv12I GWGCWC 2 cut(s) 282, 495
BbvI GCAGC 2 cut(s) 892, 1017
BccI CCATC 3 cut(s) 112, 851, 863
BceAI ACGGC 1 cut(s) 265
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 1 cut(s) 924
BcnI CCSGG 1 cut(s) 254
BcoDI GTCTC 4 cut(s) 139, 394, 479, 938
BfaI CTAG 1 cut(s) 999
BisI GCNGC 2 cut(s) 906, 1006
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 2 cut(s) 907, 1007
Bme1390I CCNGG 2 cut(s) 254, 924
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 198, 251
BmrFI CCNGG 2 cut(s) 254, 924
BmrI ACTGGG 2 cut(s) 205, 579
BmuI ACTGGG 2 cut(s) 205, 579
BpiI GAAGAC 1 cut(s) 838
BpmI CTGGAG 2 cut(s) 164, 377
Bpu10I CCTNAGC 1 cut(s) 713
Bpu1102I GCTNAGC 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 254
BsaI GGTCTC 2 cut(s) 139, 394
BsaJI CCNNGG 1 cut(s) 476
BsaWI WCCGGW 1 cut(s) 1061
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 2 cut(s) 222, 742
Bse1I ACTGG 5 cut(s) 200, 290, 574, 619, 858
Bse3DI GCAATG 2 cut(s) 191, 630
BseBI CCWGG 1 cut(s) 924
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 4 cut(s) 706, 751, 862, 883
BseLI CCNNNNNNNGG 2 cut(s) 222, 742
BseMI GCAATG 2 cut(s) 191, 630
BseMII CTCAG 7 cut(s) 134, 174, 186, 231, 255, 286, 480
BseNI ACTGG 5 cut(s) 200, 290, 574, 619, 858
BseRI GAGGAG 5 cut(s) 51, 160, 374, 412, 545
BseXI GCAGC 2 cut(s) 892, 1017
BshFI GGCC 3 cut(s) 200, 252, 481
BsiHKAI GWGCWC 2 cut(s) 282, 495
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 3 cut(s) 254, 482, 1062
BslFI GGGAC 3 cut(s) 580, 625, 633
BslI CCNNNNNNNGG 2 cut(s) 222, 742
BsmAI GTCTC 4 cut(s) 139, 394, 479, 938
BsmFI GGGAC 3 cut(s) 580, 625, 633
BsnI GGCC 3 cut(s) 200, 252, 481
Bso31I GGTCTC 2 cut(s) 139, 394
BsoBI CYCGRG 1 cut(s) 808
Bsp1286I GDGCHC 2 cut(s) 282, 495
Bsp143I GATC 2 cut(s) 722, 880
Bsp1720I GCTNAGC 1 cut(s) 8
BspACI CCGC 3 cut(s) 668, 861, 1040
BspANI GGCC 3 cut(s) 200, 252, 481
BspCNI CTCAG 7 cut(s) 135, 173, 185, 230, 254, 285, 481
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 1 cut(s) 88
BspTNI GGTCTC 2 cut(s) 139, 394
BsrDI GCAATG 2 cut(s) 191, 630
BsrI ACTGG 5 cut(s) 200, 290, 574, 619, 858
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 2 cut(s) 722, 880
Bst2UI CCWGG 1 cut(s) 924
Bst4CI ACNGT 2 cut(s) 628, 786
Bst6I CTCTTC 2 cut(s) 88, 387
BstDEI CTNAG 9 cut(s) 8, 143, 160, 172, 217, 241, 272, 489, 713
BstF5I GGATG 4 cut(s) 706, 751, 862, 883
BstKTI GATC 2 cut(s) 725, 883
BstMAI GTCTC 4 cut(s) 139, 394, 479, 938
BstMBI GATC 2 cut(s) 722, 880
BstMWI GCNNNNNNNGC 3 cut(s) 78, 277, 286
BstNI CCWGG 1 cut(s) 924
BstSCI CCNGG 2 cut(s) 252, 922
BstV1I GCAGC 2 cut(s) 892, 1017
BstV2I GAAGAC 1 cut(s) 838
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BsuRI GGCC 3 cut(s) 200, 252, 481
BtsCI GGATG 4 cut(s) 706, 751, 862, 883
BtsI GCAGTG 2 cut(s) 651, 968
BtsIMutI CAGTG 3 cut(s) 651, 851, 968
CaiI CAGNNNCTG 1 cut(s) 1011
Cfr13I GGNCC 2 cut(s) 198, 251
CseI GACGC 1 cut(s) 308
CsiI ACCWGGT 1 cut(s) 922
Csp6I GTAC 4 cut(s) 367, 412, 787, 982
CviAII CATG 3 cut(s) 836, 874, 985
CviQI GTAC 4 cut(s) 367, 412, 787, 982
DdeI CTNAG 9 cut(s) 8, 143, 160, 172, 217, 241, 272, 489, 713
DpnI GATC 2 cut(s) 724, 882
DpnII GATC 2 cut(s) 722, 880
Eam1104I CTCTTC 2 cut(s) 88, 387
EarI CTCTTC 2 cut(s) 88, 387
Ecl136II GAGCTC 2 cut(s) 280, 493
Eco24I GRGCYC 2 cut(s) 282, 495
Eco31I GGTCTC 2 cut(s) 139, 394
Eco53kI GAGCTC 2 cut(s) 280, 493
Eco57I CTGAAG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 808
EcoICRI GAGCTC 2 cut(s) 280, 493
EcoRII CCWGG 1 cut(s) 922
EcoT38I GRGCYC 2 cut(s) 282, 495
FaeI CATG 3 cut(s) 839, 877, 988
FaiI YATR 7 cut(s) 690, 791, 837, 875, 986, 1025, 1050
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 3 cut(s) 580, 625, 633
FatI CATG 3 cut(s) 835, 873, 984
FauI CCCGC 2 cut(s) 661, 1033
FblI GTMKAC 2 cut(s) 227, 294
Fnu4HI GCNGC 2 cut(s) 906, 1006
FokI GGATG 4 cut(s) 713, 758, 869, 890
FriOI GRGCYC 2 cut(s) 282, 495
Fsp4HI GCNGC 2 cut(s) 906, 1006
FspBI CTAG 1 cut(s) 999
GluI GCNGC 2 cut(s) 906, 1006
GsuI CTGGAG 2 cut(s) 164, 377
HaeIII GGCC 3 cut(s) 200, 252, 481
HapII CCGG 3 cut(s) 254, 482, 1062
HgaI GACGC 1 cut(s) 308
Hin1II CATG 3 cut(s) 839, 877, 988
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 7 cut(s) 239, 257, 315, 352, 472, 646, 1065
HpaII CCGG 3 cut(s) 254, 482, 1062
HphI GGTGA 6 cut(s) 81, 99, 524, 532, 539, 965
Hpy166II GTNNAC 4 cut(s) 228, 295, 695, 758
Hpy188I TCNGA 5 cut(s) 144, 175, 244, 275, 477
Hpy188III TCNNGA 3 cut(s) 356, 458, 1031
Hpy8I GTNNAC 4 cut(s) 228, 295, 695, 758
Hpy99I CGWCG 2 cut(s) 72, 302
HpyAV CCTTC 5 cut(s) 43, 241, 455, 517, 746
HpyCH4III ACNGT 2 cut(s) 628, 786
HpyCH4V TGCA 5 cut(s) 635, 764, 851, 897, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 277, 286
HpyF3I CTNAG 9 cut(s) 8, 143, 160, 172, 217, 241, 272, 489, 713
Hsp92II CATG 3 cut(s) 839, 877, 988
Kzo9I GATC 2 cut(s) 722, 880
LguI GCTCTTC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 361, 709
Lsp1109I GCAGC 2 cut(s) 892, 1017
MabI ACCWGGT 1 cut(s) 922
MaeI CTAG 1 cut(s) 999
MaeIII GTNAC 2 cut(s) 797, 1057
MalI GATC 2 cut(s) 724, 882
MboI GATC 2 cut(s) 722, 880
MboII GAAGA 3 cut(s) 75, 404, 843
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 2 cut(s) 282, 495
MluCI AATT 3 cut(s) 336, 1015, 1026
MlyI GAGTC 4 cut(s) 233, 251, 361, 655
MmeI TCCRAC 1 cut(s) 808
MseI TTAA 4 cut(s) 39, 539, 675, 740
MspI CCGG 3 cut(s) 254, 482, 1062
MspR9I CCNGG 2 cut(s) 254, 924
MvaI CCWGG 1 cut(s) 924
MwoI GCNNNNNNNGC 3 cut(s) 78, 277, 286
NciI CCSGG 1 cut(s) 254
NdeII GATC 2 cut(s) 722, 880
NlaIII CATG 3 cut(s) 839, 877, 988
NmuCI GTSAC 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 88
PfeI GAWTC 3 cut(s) 315, 472, 1065
PkrI GCNGC 2 cut(s) 907, 1007
PleI GAGTC 4 cut(s) 233, 251, 360, 654
PpsI GAGTC 4 cut(s) 233, 251, 360, 654
Psp124BI GAGCTC 2 cut(s) 282, 495
Psp6I CCWGG 1 cut(s) 922
PspGI CCWGG 1 cut(s) 922
PspPI GGNCC 2 cut(s) 198, 251
PstNI CAGNNNCTG 1 cut(s) 1011
PsuI RGATCY 1 cut(s) 722
RsaI GTAC 4 cut(s) 368, 413, 788, 983
RsaNI GTAC 4 cut(s) 367, 412, 787, 982
SacI GAGCTC 2 cut(s) 282, 495
SalI GTCGAC 1 cut(s) 226
SapI GCTCTTC 1 cut(s) 88
SaqAI TTAA 4 cut(s) 39, 539, 675, 740
SatI GCNGC 2 cut(s) 906, 1006
Sau3AI GATC 2 cut(s) 722, 880
Sau96I GGNCC 2 cut(s) 198, 251
SchI GAGTC 4 cut(s) 233, 251, 361, 655
ScrFI CCNGG 2 cut(s) 254, 924
SduI GDGCHC 2 cut(s) 282, 495
SexAI ACCWGGT 1 cut(s) 922
Sse9I AATT 3 cut(s) 336, 1015, 1026
SsiI CCGC 3 cut(s) 668, 861, 1040
SspMI CTAG 1 cut(s) 999
SstI GAGCTC 2 cut(s) 282, 495
StyD4I CCNGG 2 cut(s) 252, 922
TaaI ACNGT 2 cut(s) 628, 786
TaqI TCGA 3 cut(s) 227, 260, 318
TasI AATT 3 cut(s) 336, 1015, 1026
TatI WGTACW 3 cut(s) 366, 411, 981
TfiI GAWTC 3 cut(s) 315, 472, 1065
Tru1I TTAA 4 cut(s) 39, 539, 675, 740
Tru9I TTAA 4 cut(s) 39, 539, 675, 740
TscAI CASTG 3 cut(s) 658, 858, 968
TseFI GTSAC 1 cut(s) 797
TseI GCWGC 2 cut(s) 905, 1005
Tsp45I GTSAC 1 cut(s) 797
TspDTI ATGAA 4 cut(s) 17, 75, 852, 1037
TspGWI ACGGA 2 cut(s) 56, 1058
TspRI CASTG 3 cut(s) 658, 858, 968
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XmiI GTMKAC 2 cut(s) 227, 294
XspI CTAG 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.