Rh4BG038500

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003404
Physical Location & Seq
Reverse (-)
6920482 .. 6924412
3931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG038500.1

Sequence Viewer

Length: 396 bp
ATGATTCAATGGGGAGAGCATTGGAAGATACTGGACTATTCATGCAATGAAGGGGTTTTGATTCTGAGGTCGGAGACTGAGCTCATTGTTGATTTGGTGGGTGATGTGGTGAAGGATGAGGAGGGTTTCAGAGAGGGTTTGTGGGCATATTTGGGGACTGGGAGTGGGGCTATTGCTATTGGGATTGGGAGGGTTTTGGGGACTGGTGTCAGAGTCATTACAACTGATTTGAGTCTCATTGCGATTTCGGTTGCGGGTTTTAATGTGGAGAGGGAGTTGGTACTTGGATTTTGCCTCATCTTATGGATGGCTGCGCTTGTAATGATCATCAAGAAAGATAAGCACTTGAATAAGGTTGATGCTGTAGATGATGGAGATGTGCGCACATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

131

Amino Acids

14.29

Weight (kDa)

4.7

Isoelectric Point (pI)

21.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
AciI CCGC 1 cut(s) 254
AfaI GTAC 1 cut(s) 282
AgsI TTSAA 2 cut(s) 8, 349
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 68, 239
ApeKI GCWGC 1 cut(s) 311
AspLEI GCGC 2 cut(s) 316, 384
AsuHPI GGTGA 2 cut(s) 113, 121
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 298
BccI CCATC 2 cut(s) 301, 365
BclI TGATCA 1 cut(s) 324
BcoDI GTCTC 2 cut(s) 68, 239
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 349
BmuI ACTGGG 1 cut(s) 168
BoxI GACNNNNGTC 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bse1I ACTGG 3 cut(s) 36, 163, 208
Bse3DI GCAATG 2 cut(s) 52, 237
BseGI GGATG 2 cut(s) 121, 312
BseMI GCAATG 2 cut(s) 52, 237
BseMII CTCAG 2 cut(s) 56, 69
BseNI ACTGG 3 cut(s) 36, 163, 208
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 84
BslFI GGGAC 2 cut(s) 169, 214
BsmAI GTCTC 2 cut(s) 68, 239
BsmFI GGGAC 2 cut(s) 169, 214
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 324
BspACI CCGC 1 cut(s) 254
BspCNI CTCAG 2 cut(s) 57, 70
BsrDI GCAATG 2 cut(s) 52, 237
BsrI ACTGG 3 cut(s) 36, 163, 208
BssMI GATC 1 cut(s) 324
BstDEI CTNAG 3 cut(s) 65, 78, 393
BstF5I GGATG 2 cut(s) 121, 312
BstHHI GCGC 2 cut(s) 316, 384
BstKTI GATC 1 cut(s) 327
BstMAI GTCTC 2 cut(s) 68, 239
BstMBI GATC 1 cut(s) 324
BstPAI GACNNNNGTC 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 298
BtsCI GGATG 2 cut(s) 121, 312
CfoI GCGC 2 cut(s) 316, 384
Csp6I GTAC 1 cut(s) 281
CviAII CATG 1 cut(s) 42
CviJI RGCY 3 cut(s) 82, 170, 311
CviKI_1 RGCY 3 cut(s) 82, 170, 311
CviQI GTAC 1 cut(s) 281
DdeI CTNAG 3 cut(s) 65, 78, 393
DpnI GATC 1 cut(s) 326
DpnII GATC 1 cut(s) 324
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 1 cut(s) 45
FaiI YATR 3 cut(s) 43, 148, 304
FaqI GGGAC 2 cut(s) 169, 214
FatI CATG 1 cut(s) 41
FauI CCCGC 1 cut(s) 247
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 128, 319
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 312
FspAI RTGCGCAY 1 cut(s) 383
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 2 cut(s) 315, 383
GluI GCNGC 1 cut(s) 312
HhaI GCGC 2 cut(s) 316, 384
Hin1II CATG 1 cut(s) 45
Hin6I GCGC 2 cut(s) 314, 382
HinP1I GCGC 2 cut(s) 314, 382
HinfI GANTC 4 cut(s) 4, 61, 213, 232
HphI GGTGA 2 cut(s) 113, 121
Hpy188I TCNGA 4 cut(s) 66, 73, 131, 212
Hpy188III TCNNGA 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 44, 106
HpyCH4V TGCA 1 cut(s) 45
HpyF3I CTNAG 3 cut(s) 65, 78, 393
Hsp92II CATG 1 cut(s) 45
HspAI GCGC 2 cut(s) 314, 382
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 1 cut(s) 324
LpnPI CCDG 3 cut(s) 17, 144, 189
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 1 cut(s) 349
MalI GATC 1 cut(s) 326
MboI GATC 1 cut(s) 324
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 222, 241
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 7 cut(s) 60, 112, 115, 127, 183, 264, 305
MseI TTAA 1 cut(s) 261
NdeII GATC 1 cut(s) 324
NlaIII CATG 1 cut(s) 45
NsbI TGCGCA 1 cut(s) 383
PfeI GAWTC 2 cut(s) 4, 61
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 2 cut(s) 221, 240
PpsI GAGTC 2 cut(s) 221, 240
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 84
RsaI GTAC 1 cut(s) 282
RsaNI GTAC 1 cut(s) 281
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 1 cut(s) 324
SchI GAGTC 2 cut(s) 222, 241
SduI GDGCHC 1 cut(s) 84
SetI ASST 3 cut(s) 71, 84, 357
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 363
SgeI CNNG 9 cut(s) 44, 54, 171, 216, 267, 296, 329, 343, 358
SsiI CCGC 1 cut(s) 254
SstI GAGCTC 1 cut(s) 84
TfiI GAWTC 2 cut(s) 4, 61
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseI GCWGC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 30, 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.