MD05G1214300.v1.1

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003404
Physical Location & Seq
Forward (+)
34563085 .. 34564040
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1214300.v1.1.491

Sequence Viewer

Length: 327 bp
ATGTTGTGGAAGCAGAGGGTTGAGGAGAGGAGGCATTTTCAGTATGTTGTCGGGTGCGAGCATTGGAGGGACTTGGTGTTGTGTGTTGAAGAAGGGGTTTTGATTCCGAGGCCGGAGACTGAGCTCATTGTTGATTTGGTGGGGGGTGTGGTTTTGGGGAATGATGGGTTGTGGGCTGATTTGGGGACGGGGAGCGGCGCCATTGCGATTGGGATTGTGAGGGTTTTGGGGAGTGGTGGGAGAGTCATTGCTACGGATTTGAGTCCCGCGGCAATTGGGGTTGCAGCTTTTAATGTGCAGGGGTATGGTTTGCAGGTTAGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

109

Amino Acids

11.51

Weight (kDa)

4.97

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 304
AccB1I GGYRCC 1 cut(s) 197
AccBSI CCGCTC 1 cut(s) 195
AccII CGCG 1 cut(s) 269
AciI CCGC 3 cut(s) 195, 267, 269
AcyI GRCGYC 1 cut(s) 198
AgsI TTSAA 1 cut(s) 89
AluBI AGCT 2 cut(s) 124, 287
AluI AGCT 2 cut(s) 124, 287
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 296
BccI CCATC 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 201
BfuAI ACCTGC 1 cut(s) 304
BisI GCNGC 3 cut(s) 196, 270, 285
BlsI GCNGC 3 cut(s) 197, 271, 286
BmiI GGNNCC 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 107, 267
Bse3DI GCAATG 2 cut(s) 201, 246
BseDI CCNNGG 2 cut(s) 107, 267
BseMI GCAATG 2 cut(s) 201, 246
BseMII CTCAG 1 cut(s) 111
BseRI GAGGAG 2 cut(s) 38, 43
BseXI GCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 1 cut(s) 112
BshNI GGYRCC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 113
BslFI GGGAC 3 cut(s) 83, 199, 249
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 3 cut(s) 83, 199, 249
BsnI GGCC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 126
BspACI CCGC 3 cut(s) 195, 267, 269
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 1 cut(s) 112
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 304
BspT107I GGYRCC 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 2 cut(s) 201, 246
BssECI CCNNGG 2 cut(s) 107, 267
BssNI GRCGYC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 267
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstMAI GTCTC 1 cut(s) 110
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 296
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 267
BveI ACCTGC 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 59
CfoI GCGC 1 cut(s) 200
Cfr42I CCGCGG 1 cut(s) 270
CviJI RGCY 4 cut(s) 112, 124, 176, 287
CviKI_1 RGCY 4 cut(s) 112, 124, 176, 287
DdeI CTNAG 1 cut(s) 120
DinI GGCGCC 1 cut(s) 199
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoT38I GRGCYC 1 cut(s) 126
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
FaiI YATR 2 cut(s) 45, 306
FaqI GGGAC 3 cut(s) 83, 199, 249
FauI CCCGC 1 cut(s) 274
Fnu4HI GCNGC 3 cut(s) 196, 270, 285
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 196, 270, 285
GlaI GCGC 1 cut(s) 199
GluI GCNGC 3 cut(s) 196, 270, 285
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 1 cut(s) 112
HapII CCGG 1 cut(s) 113
HhaI GCGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 198
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 3 cut(s) 103, 243, 262
HpaII CCGG 1 cut(s) 113
Hpy188I TCNGA 1 cut(s) 108
HpyAV CCTTC 1 cut(s) 86
HpyCH4V TGCA 3 cut(s) 284, 298, 313
HpyF3I CTNAG 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 198
HspAI GCGC 1 cut(s) 198
KasI GGCGCC 1 cut(s) 197
KspI CCGCGG 1 cut(s) 270
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 3 cut(s) 126, 284, 299
Lsp1109I GCAGC 1 cut(s) 296
MbiI CCGCTC 1 cut(s) 195
MboII GAAGA 1 cut(s) 101
MfeI CAATTG 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 273
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 252, 271
MnlI CCTC 7 cut(s) 9, 16, 21, 24, 60, 102, 213
MseI TTAA 2 cut(s) 291, 325
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 1 cut(s) 113
MunI CAATTG 1 cut(s) 273
MvnI CGCG 1 cut(s) 269
NarI GGCGCC 1 cut(s) 198
NlaIV GGNNCC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 197, 271, 286
PleI GAGTC 2 cut(s) 251, 270
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 2 cut(s) 251, 270
Psp124BI GAGCTC 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 199
SacI GAGCTC 1 cut(s) 126
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 2 cut(s) 291, 325
SatI GCNGC 3 cut(s) 196, 270, 285
SchI GAGTC 2 cut(s) 252, 271
SduI GDGCHC 1 cut(s) 126
SetI ASST 3 cut(s) 126, 289, 318
SfoI GGCGCC 1 cut(s) 199
Sfr303I CCGCGG 1 cut(s) 270
SgeI CNNG 9 cut(s) 64, 70, 85, 120, 125, 201, 278, 280, 311
SgrBI CCGCGG 1 cut(s) 270
Sse9I AATT 1 cut(s) 273
SsiI CCGC 3 cut(s) 195, 267, 269
SspDI GGCGCC 1 cut(s) 197
SstI GAGCTC 1 cut(s) 126
TasI AATT 1 cut(s) 273
TauI GCSGC 2 cut(s) 198, 272
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 291, 325
Tru9I TTAA 2 cut(s) 291, 325
TseI GCWGC 1 cut(s) 284
TspGWI ACGGA 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.