Rroxscaffold_1G00052430

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003404
Physical Location & Seq
Forward (+)
73206538 .. 73210238
3701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052430.1

Sequence Viewer

Length: 993 bp
ATGGAACAAAAGAGCAATAAAGAGACCGACTCTTTTACAACTGCAGGATTGAAACTCAAATTGCTCGAAGCTTGGATGAGCAAGGCCACTCATGTCTCCCACCTTCTCCTCTTTTCGACTTTTCTCAAGGAATTAAATAAAGTACTTTTGAACTTCACACATGACAACGGCCCCGACTCGACCCTTTTGCTCAGAGAGCTCAACTGGCTCGTAGACGACGCAGTTCAACAAGAAATCCAAACTGGTCAAAGAAAAATTAGATTGAGAGTGAGTCTGGAGGAGCTGTACTCGTTGTGGAAGCAGAGGATTGAAGAGAGGAAGCCCTTTCAGTACATTGTTGGGTGTGAGCATTGGAGGGAGTTGGTGCTGTGTGTTCAAGAAGGGGTTTTGATTCCGAGGCCGGAGACTGAGCTTATTGTCGATTTGGTGGGTGATGTGGTGAAGGGTGAGGAGGGTTTAAGAGAGGGTTTGTGGGCTGATTTGGGGACTGGGAGTGGGGCTATTGCCATTGGGATTGGAAGGGTTTTGGGGACTGGTGGGAAAGTCATTGCAACTGATTTGAGTTCAACAGCGATTTCGGTTGCGGGGTTTAATGTGGAGAGGTATGGTTTGCAGGATGTAGTGGAGCTAAGGCAAGGATCTTGGTTTGACCCATTAAAGGATGTGGAAGGTAAACTTGCGGGGTTCGTAAGTAATCCACCGTACATACCAAGTGACAATATCTCGGGGCTACAAGCTGAAGTTGGAAGACATGAACCCAGAGTTGCACTGGATGGCGGTGTTGATGGCATGGATGATCTCTTACATCTTTGCAAAGGGGCTGCTTCCATTTTGAAGCCTGGTGGATTTTTTGCTTTTGAGACAAATGGTGAGAAGCAGTGCAAGTATCTTGTGGAGTACATGGAAAATGAGGCTAGAGGCAGTTTTTTTAATTTGAGTATAATTCCTGATTTTGCGGGTATTCATAGATTTGTTACCGGATTCCGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

330

Amino Acids

36.65

Weight (kDa)

5.32

Isoelectric Point (pI)

24.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 213
AciI CCGC 4 cut(s) 584, 680, 777, 956
AclWI GGATC 1 cut(s) 646
AcuI CTGAAG 1 cut(s) 759
AfaI GTAC 5 cut(s) 144, 287, 332, 704, 899
AfiI CCNNNNNNNGG 1 cut(s) 658
AgsI TTSAA 7 cut(s) 52, 151, 227, 311, 377, 567, 835
AjnI CCWGG 1 cut(s) 838
AluBI AGCT 6 cut(s) 71, 199, 283, 412, 628, 737
AluI AGCT 6 cut(s) 71, 199, 283, 412, 628, 737
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 4 cut(s) 17, 100, 398, 854
AlwI GGATC 1 cut(s) 646
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 3 cut(s) 84, 169, 398
ApeKI GCWGC 1 cut(s) 821
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 4 cut(s) 443, 451, 458, 881
AvaI CYCGRG 1 cut(s) 724
BanII GRGCYC 1 cut(s) 201
BbsI GAAGAC 1 cut(s) 754
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 808
BccI CCATC 2 cut(s) 767, 779
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciT130I CCWGG 1 cut(s) 840
BcoDI GTCTC 4 cut(s) 17, 100, 398, 854
BfaI CTAG 1 cut(s) 915
BfmI CTRYAG 1 cut(s) 42
BisI GCNGC 1 cut(s) 822
BlsI GCNGC 1 cut(s) 823
BmcAI AGTACT 1 cut(s) 144
Bme1390I CCNGG 1 cut(s) 840
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 1 cut(s) 840
BmrI ACTGGG 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 754
BplI GAGNNNNNCTC 4 cut(s) 14, 46, 272, 304
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 629
BpuEI CTTGAG 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 395
BsaWI WCCGGW 1 cut(s) 977
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 658
Bse1I ACTGG 5 cut(s) 209, 247, 493, 538, 774
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 840
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 5 cut(s) 81, 622, 667, 778, 799
BseLI CCNNNNNNNGG 1 cut(s) 658
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 2 cut(s) 205, 399
BseNI ACTGG 5 cut(s) 209, 247, 493, 538, 774
BseRI GAGGAG 3 cut(s) 98, 293, 464
BseXI GCAGC 1 cut(s) 808
BshFI GGCC 3 cut(s) 86, 171, 400
BsiHKAI GWGCWC 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 2 cut(s) 401, 978
BslFI GGGAC 2 cut(s) 499, 544
BslI CCNNNNNNNGG 1 cut(s) 658
BsmAI GTCTC 4 cut(s) 17, 100, 398, 854
BsmFI GGGAC 2 cut(s) 499, 544
BsnI GGCC 3 cut(s) 86, 171, 400
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 638, 796
BspACI CCGC 4 cut(s) 584, 680, 777, 956
BspANI GGCC 3 cut(s) 86, 171, 400
BspCNI CTCAG 2 cut(s) 204, 400
BspLI GGNNCC 1 cut(s) 172
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 1 cut(s) 646
BspTNI GGTCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 5 cut(s) 209, 247, 493, 538, 774
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 2 cut(s) 638, 796
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 1 cut(s) 702
Bst6I CTCTTC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 191, 408, 629
BstF5I GGATG 5 cut(s) 81, 622, 667, 778, 799
BstKTI GATC 2 cut(s) 641, 799
BstMAI GTCTC 4 cut(s) 17, 100, 398, 854
BstMBI GATC 2 cut(s) 638, 796
BstMWI GCNNNNNNNGC 2 cut(s) 196, 205
BstNI CCWGG 1 cut(s) 840
BstSCI CCNGG 1 cut(s) 838
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 1 cut(s) 808
BstV2I GAAGAC 1 cut(s) 754
BstX2I RGATCY 1 cut(s) 638
BstYI RGATCY 1 cut(s) 638
BsuRI GGCC 3 cut(s) 86, 171, 400
BtsCI GGATG 5 cut(s) 81, 622, 667, 778, 799
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 2 cut(s) 767, 884
Cfr13I GGNCC 1 cut(s) 170
CseI GACGC 1 cut(s) 227
Csp6I GTAC 5 cut(s) 143, 286, 331, 703, 898
CviAII CATG 5 cut(s) 92, 161, 752, 790, 901
CviQI GTAC 5 cut(s) 143, 286, 331, 703, 898
DdeI CTNAG 3 cut(s) 191, 408, 629
DpnI GATC 2 cut(s) 640, 798
DpnII GATC 2 cut(s) 638, 796
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 17
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 724
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 838
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 5 cut(s) 95, 164, 755, 793, 904
FaiI YATR 9 cut(s) 93, 162, 606, 707, 753, 791, 902, 941, 966
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 2 cut(s) 499, 544
FatI CATG 5 cut(s) 91, 160, 751, 789, 900
FauI CCCGC 3 cut(s) 577, 673, 949
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 822
FokI GGATG 5 cut(s) 88, 629, 674, 785, 806
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 822
FspBI CTAG 1 cut(s) 915
GluI GCNGC 1 cut(s) 822
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 3 cut(s) 86, 171, 400
HapII CCGG 2 cut(s) 401, 978
HgaI GACGC 1 cut(s) 227
Hin1II CATG 5 cut(s) 95, 164, 755, 793, 904
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 5 cut(s) 29, 176, 271, 391, 981
HpaII CCGG 2 cut(s) 401, 978
HphI GGTGA 4 cut(s) 443, 451, 458, 881
Hpy166II GTNNAC 2 cut(s) 214, 674
Hpy188I TCNGA 2 cut(s) 194, 396
Hpy188III TCNNGA 3 cut(s) 275, 377, 947
Hpy8I GTNNAC 2 cut(s) 214, 674
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 5 cut(s) 113, 374, 436, 513, 662
HpyCH4III ACNGT 1 cut(s) 702
HpyCH4V TGCA 6 cut(s) 44, 551, 613, 767, 813, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 205
HpyF3I CTNAG 3 cut(s) 191, 408, 629
Hsp92II CATG 5 cut(s) 95, 164, 755, 793, 904
Kzo9I GATC 2 cut(s) 638, 796
LmnI GCTCC 2 cut(s) 280, 625
Lsp1109I GCAGC 1 cut(s) 808
MaeI CTAG 1 cut(s) 915
MaeIII GTNAC 2 cut(s) 713, 973
MalI GATC 2 cut(s) 640, 798
MboI GATC 2 cut(s) 638, 796
MboII GAAGA 2 cut(s) 323, 759
MflI RGATCY 1 cut(s) 638
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 5 cut(s) 59, 131, 255, 931, 942
MlyI GAGTC 3 cut(s) 23, 170, 280
MmeI TCCRAC 1 cut(s) 724
MseI TTAA 5 cut(s) 134, 458, 591, 656, 930
MspI CCGG 2 cut(s) 401, 978
MspR9I CCNGG 1 cut(s) 840
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 2 cut(s) 196, 205
NdeII GATC 2 cut(s) 638, 796
NlaIII CATG 5 cut(s) 95, 164, 755, 793, 904
NlaIV GGNNCC 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 713
PfeI GAWTC 2 cut(s) 391, 981
PkrI GCNGC 1 cut(s) 823
PleI GAGTC 3 cut(s) 23, 170, 279
PpsI GAGTC 3 cut(s) 23, 170, 279
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 838
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 638
RsaI GTAC 5 cut(s) 144, 287, 332, 704, 899
RsaNI GTAC 5 cut(s) 143, 286, 331, 703, 898
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 5 cut(s) 134, 458, 591, 656, 930
SatI GCNGC 1 cut(s) 822
Sau3AI GATC 2 cut(s) 638, 796
Sau96I GGNCC 1 cut(s) 170
ScaI AGTACT 1 cut(s) 144
SchI GAGTC 3 cut(s) 23, 170, 280
ScrFI CCNGG 1 cut(s) 840
SduI GDGCHC 1 cut(s) 201
SetI ASST 9 cut(s) 73, 105, 201, 285, 414, 605, 630, 673, 739
SfcI CTRYAG 1 cut(s) 42
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 5 cut(s) 59, 131, 255, 931, 942
SsiI CCGC 4 cut(s) 584, 680, 777, 956
SspMI CTAG 1 cut(s) 915
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 838
TaaI ACNGT 1 cut(s) 702
TaqI TCGA 4 cut(s) 66, 116, 179, 420
TaqII GACCGA 1 cut(s) 41
TasI AATT 5 cut(s) 59, 131, 255, 931, 942
TatI WGTACW 4 cut(s) 142, 285, 330, 897
TfiI GAWTC 2 cut(s) 391, 981
Tru1I TTAA 5 cut(s) 134, 458, 591, 656, 930
Tru9I TTAA 5 cut(s) 134, 458, 591, 656, 930
TscAI CASTG 2 cut(s) 774, 884
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 1 cut(s) 821
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 2 cut(s) 768, 953
TspGWI ACGGA 1 cut(s) 974
TspRI CASTG 2 cut(s) 774, 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 1 cut(s) 915
ZrmI AGTACT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.