Rorug02G0601800

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003404
Physical Location & Seq
Forward (+)
72045106 .. 72049962
4857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0601800.1

Sequence Viewer

Length: 2676 bp
ATGTTCCAGTTGAAAAATGTCCAGTTCTTCGCCGTACTTGATGTTGAAATTGATGCTTTCCTAGTCCAACTAGGTAACACAACTTACGAGGTGGGCAGATTGCAGAGCCAATCTATGTCGCTGCATGCCTTGGATTCAAACAAGAACAAAATAATGTCCTTCCTCTACTTGGCTTGTGCATCATGGCTTACTTGTTTCCTAGAGGGGTTTTGTTGGACAAGAACTGGGGAGAGACAAGCTGCAAAAATGAGAACAAAGTATTTGAAAGCGGTGTTACGCCAAGAGGTGCCAGACTTCTTAACTAGAGTCTCTAGTTTCATTGCAAGTTACGCGATAGCATTTGTATTGATGTGGGAACTGGCTTTGGTTGGACTCCCCTTTATATTGCTTCTGGTAATTCTTGGTTGGATTTGTGGAAGAACCTTAACACGAATAGCAGGGACGAGAAGACAAGAACAAATTAAGGCGGCGACGATAGCAGAACAGGCAATATCATCTATTAGAACAGTTTATGCATTTGTTGGAGAAAGCAATACTATAACACAATTTTCTGCAGCTCTACATGGGAATACAAAGCTTGGGTTGAAGCAAGGATTGGTTAAAGGCTTGGAAATTGGTAGCACCTATTCTGTCTTTGCCTTTTGGGCTGTCATGTCTTATTTAGGTAGCAGAATGGTTATATACCATGGTGCTCTAGGAGGGACTGTTTTTGCTGTTGGTGTTTCACTGGTCAGTGGAGCAATGGCAATGGGTTTTGCTTTATCAAACTTAAAGTACATTGCAGAAGTATGCTCAGCTGAAGAGAATCTCATGAAGGTTATCAATCGAGTTCCTGAAATTGATTCAGAAAACATGGAAGGCACAATTCCGGACGATGTCTTGAGTGAAGTCCAATTTAATCATGTTAAGTTTGCATATCCATCCAGGCCTGAGAGCATTATTCTGAACAACTTTTCTCTAACCATACCAGCCGGAAAGACTGTTGCCTTGGTCGGCTCAAGTGGATCAGGAAAATCAACAGTGACATTACTGATGGAAAGATTTTATGACCCACTTGAGGGACAAATTCTACTAGATGGGTTTGCCATTGACAAGTTACAGCTCAAGTGGCTAAGGTCTAAAATGGCATCCGTAAACCAAGAGCCTTCATTGTTCTCAACCACCATTAAGGAGAATATACTTTTCGGCAAGGAGGATGGCACAATGGAGGAAGTAGTTGAGGCTGCCAAGGCTAGCAATGCTCATAATTTCATATGTCAGTTGCCTCATGGGTATGACACCCAGGTTGGTGAGAGAGGCATTCAAGTATCAAGTGGACAGAAGCAGAGAATAGCAATAGCCAGAGCACTTATAAAGAAACCCCAAATCCTTCTCCTTGATGAGGCCACCAGTGCATTAGACTCAGAGTCAGAAAGACTCGTTCAAGAAGCCCTCCAGACAGCAGCCACAGGAAGAACCACCATAGTCATTGCTCACCGCTTGTCCACCATTCAGAAGGCTGATAAAGATGGTATTTACTCCTCATTTGTCCGCCTCCAACAAACGCCACAAAAGGAGGCACCAGAAGATCAAGGTCATTGGAACTATAGTGCTAGTACTAGACAGTTGGACTCAACCTCCCAGCTTGTTGTATCTAATAGTACTAGTCCCACTGCTCAAGAATCTACAAGCGACCTTTCCAAGCAGAAATCTTCACTTTGGAGATTGTTATCTATGAATATTCCAGAGTGGAAGCAAGCAGTGTTAGGTTGGTTATCTGCAATTCTTTTTGGAGCTGTTGAACATGTGTTTCAATTGACCTTGGGAACTACATTATCCATCTTTTTCTTGACAAATACCGAGGAGATTAAGGAGAAAATCACAACTCTCGCTATCTGCTTTTGCGGGTTGTCTGTGTTTGCATCGCTGATTACTCTCCTGCAACACTATAGTTTTGCATACACTGGGGAGCATTTGAGTAATCGGATTAGAGAAAGCTTGCTCACGAAAATACTTAGTTTTGAAGTTGGCTGGTTCGATGACGATAGGAATTCTAGCGGTGCCATTTGCTCTAAACTCACCAGAGATGCTGAACAAGTGAGATCTTTAGTTGGTGATAGAATGTGTCTTCTTGTCCAAACCATCTCAGGTGTAACTATAGCTTGGACAATGAGCCTAATAATTGCATGGAGGCTTGCCGTAGTCATCATTGCGCTCCAACCCATAGTCATTTTCACCCTTTATTCTAGGCGTGTCCTGCTCGGAAGAATGTCCAGCAAAGCCATTAAAAGCCAAGAGGAAAGTAGCAAGCTTGCAGCTGAAGCTATCTACAACCACCGGACCGTAACTGCCTTCTCAGCTCAGAGCAAGATTCTGAAAATGCTAGAAAAGGTCCAAGAAGGCCCACATAGAGAAATCTTACGACAATCGTGGTTTGCCGGTATTGGGCTCGGGTTTTCCATGAGCATTGCAATGCTCATTGCTGGTTTAAGCTATTGGTATGGCAACATGCTCGTCTCCCATGGCTACCTCACACCAAGATCAGCCTTTCAAACCATTCTCATTTTATTAGTCACGGGTCGGGTCATAGCAGATGCTGGAAGCATGACATCAGACCTTACCAATGGCTGGGAAGCAATTGGGTCAATTTTCAGTATCTTGGACCGAACCCCAAGTTCCAAAAGACTCCCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

891

Amino Acids

98.37

Weight (kDa)

8.58

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 51 - 99 7.5e-07 ABC transporter transmembrane region
ABC_membrane PF00664 97 - 247 6.4e-18 ABC transporter transmembrane region
ABC_tran PF00005 315 - 464 4.4e-32 ABC transporter
ABC_membrane PF00664 584 - 851 7.6e-43 ABC transporter transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1352
AccB1I GGYRCC 3 cut(s) 286, 1558, 2039
AccB7I CCANNNNNTGG 1 cut(s) 2607
AccII CGCG 1 cut(s) 332
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 6 cut(s) 269, 467, 1477, 1531, 1884, 2037
AclWI GGATC 1 cut(s) 1012
AcsI RAATTY 2 cut(s) 1065, 2029
AcuI CTGAAG 2 cut(s) 819, 2319
AfaI GTAC 4 cut(s) 36, 776, 1597, 1642
AfiI CCNNNNNNNGG 6 cut(s) 169, 1057, 1058, 1381, 2424, 2607
AflIII ACRYGT 1 cut(s) 1783
AhdI GACNNNNNGTC 1 cut(s) 1405
AhlI ACTAGT 1 cut(s) 1643
AjnI CCWGG 2 cut(s) 923, 1281
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 2 cut(s) 694, 1348
Alw26I GTCTC 3 cut(s) 226, 313, 2500
AlwI GGATC 1 cut(s) 1012
AlwNI CAGNNNCTG 1 cut(s) 2576
Ama87I CYCGRG 1 cut(s) 2429
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 3 cut(s) 926, 1383, 2380
ApeKI GCWGC 6 cut(s) 121, 239, 554, 1223, 1442, 2294
ApoI RAATTY 2 cut(s) 1065, 2029
AspLEI GCGC 1 cut(s) 2194
AspS9I GGNCC 4 cut(s) 2319, 2371, 2381, 2641
AsuHPI GGTGA 5 cut(s) 1301, 1466, 2050, 2105, 2206
AsuNHI GCTAGC 1 cut(s) 1232
AvaI CYCGRG 1 cut(s) 2429
AvaII GGWCC 3 cut(s) 2319, 2371, 2641
BanI GGYRCC 3 cut(s) 286, 1558, 2039
BanII GRGCYC 1 cut(s) 2430
BbsI GAAGAC 2 cut(s) 454, 2099
Bbv12I GWGCWC 2 cut(s) 694, 1348
BbvI GCAGC 6 cut(s) 108, 226, 566, 1210, 1454, 2306
BccI CCATC 7 cut(s) 928, 1027, 1070, 1190, 1502, 1826, 2129
BceAI ACGGC 2 cut(s) 17, 2162
BcgI CGANNNNNNTGC 2 cut(s) 2473, 2507
BciT130I CCWGG 2 cut(s) 925, 1283
BcoDI GTCTC 3 cut(s) 226, 313, 2500
BcuI ACTAGT 1 cut(s) 1643
BfmI CTRYAG 4 cut(s) 552, 1585, 1927, 2136
BglI GCCNNNNNGGC 1 cut(s) 644
BglII AGATCT 1 cut(s) 2081
BisI GCNGC 7 cut(s) 122, 240, 468, 555, 1224, 1443, 2295
BlpI GCTNAGC 1 cut(s) 793
BlsI GCNGC 7 cut(s) 123, 241, 469, 556, 1225, 1444, 2296
BmcAI AGTACT 2 cut(s) 1597, 1642
Bme1390I CCNGG 2 cut(s) 925, 1283
Bme18I GGWCC 3 cut(s) 2319, 2371, 2641
BmeRI GACNNNNNGTC 1 cut(s) 1405
BmeT110I CYCGRG 1 cut(s) 2429
BmgT120I GGNCC 4 cut(s) 2319, 2371, 2381, 2641
BmiI GGNNCC 3 cut(s) 288, 1560, 2041
BmrFI CCNGG 2 cut(s) 925, 1283
BmrI ACTGGG 2 cut(s) 234, 1953
BmsI GCATC 6 cut(s) 43, 188, 1136, 1910, 2056, 2563
BmtI GCTAGC 1 cut(s) 1236
BmuI ACTGGG 2 cut(s) 234, 1953
BpiI GAAGAC 2 cut(s) 454, 2099
BpmI CTGGAG 1 cut(s) 1418
Bpu10I CCTNAGC 1 cut(s) 1112
Bpu1102I GCTNAGC 1 cut(s) 793
BpuEI CTTGAG 5 cut(s) 901, 982, 1076, 1088, 1641
BsaBI GATNNNNATC 1 cut(s) 1708
BsaJI CCNNGG 8 cut(s) 129, 685, 987, 1227, 1281, 1800, 1839, 2500
BsaWI WCCGGW 2 cut(s) 868, 2316
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 1601, 1631
Bsc4I CCNNNNNNNGG 6 cut(s) 169, 1057, 1058, 1381, 2424, 2607
Bse118I RCCGGY 1 cut(s) 2417
Bse1I ACTGG 7 cut(s) 7, 22, 229, 363, 732, 1389, 1948
Bse8I GATNNNNATC 1 cut(s) 1708
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 2 cut(s) 925, 1283
BseDI CCNNGG 8 cut(s) 129, 685, 987, 1227, 1281, 1800, 1839, 2500
BseGI GGATG 3 cut(s) 920, 1127, 1201
BseJI GATNNNNATC 1 cut(s) 1708
BseLI CCNNNNNNNGG 6 cut(s) 169, 1057, 1058, 1381, 2424, 2607
BseMII CTCAG 6 cut(s) 807, 921, 1416, 2139, 2349, 2354
BseNI ACTGG 7 cut(s) 7, 22, 229, 363, 732, 1389, 1948
BseRI GAGGAG 2 cut(s) 1510, 1856
BseXI GCAGC 6 cut(s) 108, 226, 566, 1210, 1454, 2306
BseYI CCCAGC 2 cut(s) 1620, 2607
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 3 cut(s) 928, 1385, 2382
BshNI GGYRCC 3 cut(s) 286, 1558, 2039
BsiHKAI GWGCWC 2 cut(s) 694, 1348
BsiHKCI CYCGRG 1 cut(s) 2429
BsiSI CCGG 4 cut(s) 869, 972, 2317, 2418
BslFI GGGAC 4 cut(s) 454, 715, 1074, 1632
BslI CCNNNNNNNGG 6 cut(s) 169, 1057, 1058, 1381, 2424, 2607
BsmAI GTCTC 3 cut(s) 226, 313, 2500
BsmBI CGTCTC 1 cut(s) 2500
BsmFI GGGAC 4 cut(s) 454, 715, 1074, 1632
BsmI GAATGC 1 cut(s) 1299
BsnI GGCC 3 cut(s) 928, 1385, 2382
BsoBI CYCGRG 1 cut(s) 2429
Bsp1286I GDGCHC 3 cut(s) 694, 1348, 2430
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 4 cut(s) 1004, 1567, 2081, 2519
Bsp1720I GCTNAGC 1 cut(s) 793
Bsp19I CCATGG 2 cut(s) 685, 2500
BspACI CCGC 6 cut(s) 269, 467, 1477, 1531, 1884, 2037
BspANI GGCC 3 cut(s) 928, 1385, 2382
BspCNI CTCAG 6 cut(s) 806, 922, 1415, 2138, 2348, 2353
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 1 cut(s) 332
BspHI TCATGA 1 cut(s) 810
BspLI GGNNCC 3 cut(s) 288, 1560, 2041
BspMAI CTGCAG 1 cut(s) 556
BspOI GCTAGC 1 cut(s) 1236
BspPI GGATC 1 cut(s) 1012
BspT107I GGYRCC 3 cut(s) 286, 1558, 2039
BsrFI RCCGGY 1 cut(s) 2417
BsrI ACTGG 7 cut(s) 7, 22, 229, 363, 732, 1389, 1948
BssAI RCCGGY 1 cut(s) 2417
BssECI CCNNGG 8 cut(s) 129, 685, 987, 1227, 1281, 1800, 1839, 2500
BssMI GATC 4 cut(s) 1004, 1567, 2081, 2519
BssT1I CCWWGG 6 cut(s) 129, 685, 987, 1227, 1800, 2500
Bst2UI CCWGG 2 cut(s) 925, 1283
Bst4CI ACNGT 6 cut(s) 508, 706, 982, 1021, 1605, 2323
Bst6I CTCTTC 1 cut(s) 795
BstC8I GCNNGC 7 cut(s) 126, 1234, 1737, 1979, 2175, 2288, 2292
BstDEI CTNAG 8 cut(s) 793, 930, 1112, 1402, 1994, 2125, 2335, 2340
BstDSI CCRYGG 2 cut(s) 685, 2500
BstENI CCTNNNNNAGG 1 cut(s) 1379
BstF5I GGATG 3 cut(s) 920, 1127, 1201
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 2194
BstKTI GATC 4 cut(s) 1007, 1570, 2084, 2522
BstMAI GTCTC 3 cut(s) 226, 313, 2500
BstMBI GATC 4 cut(s) 1004, 1567, 2081, 2519
BstNI CCWGG 2 cut(s) 925, 1283
BstNSI RCATGY 3 cut(s) 128, 1787, 2491
BstSCI CCNGG 2 cut(s) 923, 1281
BstSFI CTRYAG 4 cut(s) 552, 1585, 1927, 2136
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 6 cut(s) 108, 226, 566, 1210, 1454, 2306
BstV2I GAAGAC 2 cut(s) 454, 2099
BstX2I RGATCY 1 cut(s) 2081
BstYI RGATCY 1 cut(s) 2081
BsuRI GGCC 3 cut(s) 928, 1385, 2382
BtgI CCRYGG 2 cut(s) 685, 2500
BtgZI GCGATG 1 cut(s) 1887
BtsCI GGATG 3 cut(s) 920, 1127, 1201
BtsI GCAGTG 2 cut(s) 1650, 1746
BtsIMutI CAGTG 7 cut(s) 725, 739, 1026, 1396, 1650, 1746, 1941
Cac8I GCNNGC 7 cut(s) 126, 1234, 1737, 1979, 2175, 2288, 2292
CaiI CAGNNNCTG 1 cut(s) 2576
CciI TCATGA 1 cut(s) 810
CfoI GCGC 1 cut(s) 2194
Cfr10I RCCGGY 1 cut(s) 2417
Cfr13I GGNCC 4 cut(s) 2319, 2371, 2381, 2641
CpoI CGGWCCG 1 cut(s) 2319
Csp6I GTAC 4 cut(s) 35, 775, 1596, 1641
CspI CGGWCCG 1 cut(s) 2319
CviQI GTAC 4 cut(s) 35, 775, 1596, 1641
DdeI CTNAG 8 cut(s) 793, 930, 1112, 1402, 1994, 2125, 2335, 2340
DpnI GATC 4 cut(s) 1006, 1569, 2083, 2521
DpnII GATC 4 cut(s) 1004, 1567, 2081, 2519
DriI GACNNNNNGTC 1 cut(s) 1405
Eam1104I CTCTTC 1 cut(s) 795
Eam1105I GACNNNNNGTC 1 cut(s) 1405
EarI CTCTTC 1 cut(s) 795
EciI GGCGGA 1 cut(s) 1520
Eco130I CCWWGG 6 cut(s) 129, 685, 987, 1227, 1800, 2500
Eco147I AGGCCT 1 cut(s) 928
Eco24I GRGCYC 1 cut(s) 2430
Eco47I GGWCC 3 cut(s) 2319, 2371, 2641
Eco57I CTGAAG 2 cut(s) 819, 2319
Eco88I CYCGRG 1 cut(s) 2429
EcoNI CCTNNNNNAGG 1 cut(s) 1379
EcoRI GAATTC 1 cut(s) 2029
EcoRII CCWGG 2 cut(s) 923, 1281
EcoT14I CCWWGG 6 cut(s) 129, 685, 987, 1227, 1800, 2500
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 2430
ErhI CCWWGG 6 cut(s) 129, 685, 987, 1227, 1800, 2500
Esp3I CGTCTC 1 cut(s) 2500
FaqI GGGAC 4 cut(s) 454, 715, 1074, 1632
FauI CCCGC 1 cut(s) 1877
FauNDI CATATG 1 cut(s) 1253
Fnu4HI GCNGC 7 cut(s) 122, 240, 468, 555, 1224, 1443, 2295
FokI GGATG 3 cut(s) 907, 1114, 1208
FriOI GRGCYC 1 cut(s) 2430
Fsp4HI GCNGC 7 cut(s) 122, 240, 468, 555, 1224, 1443, 2295
GlaI GCGC 1 cut(s) 2193
GluI GCNGC 7 cut(s) 122, 240, 468, 555, 1224, 1443, 2295
GsaI CCCAGC 2 cut(s) 1624, 2611
GsuI CTGGAG 1 cut(s) 1418
HaeIII GGCC 3 cut(s) 928, 1385, 2382
HapII CCGG 4 cut(s) 869, 972, 2317, 2418
HhaI GCGC 1 cut(s) 2194
Hin6I GCGC 1 cut(s) 2192
HinP1I GCGC 1 cut(s) 2192
HindIII AAGCTT 3 cut(s) 575, 1975, 2288
HpaII CCGG 4 cut(s) 869, 972, 2317, 2418
HphI GGTGA 5 cut(s) 1301, 1466, 2050, 2105, 2206
Hpy166II GTNNAC 3 cut(s) 1135, 1316, 1485
Hpy8I GTNNAC 3 cut(s) 1135, 1316, 1485
Hpy99I CGWCG 1 cut(s) 475
HpyAV CCTTC 8 cut(s) 169, 808, 851, 1155, 1379, 1489, 2341, 2372
HpyCH4III ACNGT 6 cut(s) 508, 706, 982, 1021, 1605, 2323
HpyF3I CTNAG 8 cut(s) 793, 930, 1112, 1402, 1994, 2125, 2335, 2340
HspAI GCGC 1 cut(s) 2192
Kpn2I TCCGGA 1 cut(s) 868
Kzo9I GATC 4 cut(s) 1004, 1567, 2081, 2519
LmnI GCTCC 4 cut(s) 737, 1772, 1948, 2199
Lsp1109I GCAGC 6 cut(s) 108, 226, 566, 1210, 1454, 2306
LweI GCATC 6 cut(s) 43, 188, 1136, 1910, 2056, 2563
MaeIII GTNAC 8 cut(s) 74, 273, 326, 1021, 1095, 2131, 2323, 2551
MalI GATC 4 cut(s) 1006, 1569, 2083, 2521
MboI GATC 4 cut(s) 1004, 1567, 2081, 2519
MboII GAAGA 9 cut(s) 19, 429, 459, 812, 1464, 1577, 1683, 2099, 2256
MfeI CAATTG 2 cut(s) 1793, 2616
MflI RGATCY 1 cut(s) 2081
MhlI GDGCHC 3 cut(s) 694, 1348, 2430
MlyI GAGTC 7 cut(s) 315, 366, 1394, 1410, 1415, 1604, 2658
MmeI TCCRAC 8 cut(s) 91, 194, 349, 386, 502, 1561, 1587, 2221
Mph1103I ATGCAT 1 cut(s) 517
MroI TCCGGA 1 cut(s) 868
MslI CAYNNNNRTG 2 cut(s) 1272, 2450
MspA1I CMGCKG 2 cut(s) 797, 2297
MspI CCGG 4 cut(s) 869, 972, 2317, 2418
MspR9I CCNGG 2 cut(s) 925, 1283
MunI CAATTG 2 cut(s) 1793, 2616
Mva1269I GAATGC 1 cut(s) 1299
MvaI CCWGG 2 cut(s) 925, 1283
MvnI CGCG 1 cut(s) 332
NcoI CCATGG 2 cut(s) 685, 2500
NdeI CATATG 1 cut(s) 1253
NdeII GATC 4 cut(s) 1004, 1567, 2081, 2519
NheI GCTAGC 1 cut(s) 1232
NlaIV GGNNCC 3 cut(s) 288, 1560, 2041
NmuCI GTSAC 2 cut(s) 1021, 2551
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 3 cut(s) 128, 1787, 2491
PaeI GCATGC 1 cut(s) 128
PagI TCATGA 1 cut(s) 810
PceI AGGCCT 1 cut(s) 928
PciI ACATGT 1 cut(s) 1783
PctI GAATGC 1 cut(s) 1299
PfeI GAWTC 5 cut(s) 134, 805, 842, 1661, 2350
PflFI GACNNNGTC 1 cut(s) 875
PflMI CCANNNNNTGG 1 cut(s) 2607
PkrI GCNGC 7 cut(s) 123, 241, 469, 556, 1225, 1444, 2296
PleI GAGTC 7 cut(s) 314, 366, 1394, 1410, 1414, 1604, 2658
PpsI GAGTC 7 cut(s) 314, 366, 1394, 1410, 1414, 1604, 2658
PscI ACATGT 1 cut(s) 1783
PsiI TTATAA 1 cut(s) 1352
Psp6I CCWGG 2 cut(s) 923, 1281
PspFI CCCAGC 2 cut(s) 1620, 2607
PspGI CCWGG 2 cut(s) 923, 1281
PspN4I GGNNCC 3 cut(s) 288, 1560, 2041
PspPI GGNCC 4 cut(s) 2319, 2371, 2381, 2641
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 2576
PsuI RGATCY 1 cut(s) 2081
PsyI GACNNNGTC 1 cut(s) 875
PvuII CAGCTG 2 cut(s) 797, 2297
RsaI GTAC 4 cut(s) 36, 776, 1597, 1642
RsaNI GTAC 4 cut(s) 35, 775, 1596, 1641
RseI CAYNNNNRTG 2 cut(s) 1272, 2450
Rsr2I CGGWCCG 1 cut(s) 2319
RsrII CGGWCCG 1 cut(s) 2319
SatI GCNGC 7 cut(s) 122, 240, 468, 555, 1224, 1443, 2295
Sau3AI GATC 4 cut(s) 1004, 1567, 2081, 2519
Sau96I GGNCC 4 cut(s) 2319, 2371, 2381, 2641
ScaI AGTACT 2 cut(s) 1597, 1642
SchI GAGTC 7 cut(s) 315, 366, 1394, 1410, 1415, 1604, 2658
ScrFI CCNGG 2 cut(s) 925, 1283
SduI GDGCHC 3 cut(s) 694, 1348, 2430
SfaNI GCATC 6 cut(s) 43, 188, 1136, 1910, 2056, 2563
SfcI CTRYAG 4 cut(s) 552, 1585, 1927, 2136
SinI GGWCC 3 cut(s) 2319, 2371, 2641
SmiMI CAYNNNNRTG 2 cut(s) 1272, 2450
SmlI CTYRAG 5 cut(s) 880, 997, 1055, 1103, 1656
SmoI CTYRAG 5 cut(s) 880, 997, 1055, 1103, 1656
SpeI ACTAGT 1 cut(s) 1643
SphI GCATGC 1 cut(s) 128
SseBI AGGCCT 1 cut(s) 928
SsiI CCGC 6 cut(s) 269, 467, 1477, 1531, 1884, 2037
SspI AATATT 1 cut(s) 1720
StuI AGGCCT 1 cut(s) 928
StyD4I CCNGG 2 cut(s) 923, 1281
StyI CCWWGG 6 cut(s) 129, 685, 987, 1227, 1800, 2500
TaaI ACNGT 6 cut(s) 508, 706, 982, 1021, 1605, 2323
TaqI TCGA 2 cut(s) 826, 2016
TaqII GACCGA 1 cut(s) 2658
TatI WGTACW 3 cut(s) 774, 1595, 1640
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 5 cut(s) 134, 805, 842, 1661, 2350
TscAI CASTG 7 cut(s) 732, 739, 1026, 1396, 1657, 1746, 1948
TseFI GTSAC 2 cut(s) 1021, 2551
TseI GCWGC 6 cut(s) 121, 239, 554, 1223, 1442, 2294
Tsp45I GTSAC 2 cut(s) 1021, 2551
TspDTI ATGAA 5 cut(s) 307, 827, 1137, 1240, 1730
TspGWI ACGGA 1 cut(s) 1120
TspRI CASTG 7 cut(s) 732, 739, 1026, 1396, 1657, 1746, 1948
Tth111I GACNNNGTC 1 cut(s) 875
Van91I CCANNNNNTGG 1 cut(s) 2607
VpaK11BI GGWCC 3 cut(s) 2319, 2371, 2641
XagI CCTNNNNNAGG 1 cut(s) 1379
XapI RAATTY 2 cut(s) 1065, 2029
XceI RCATGY 3 cut(s) 128, 1787, 2491
ZrmI AGTACT 2 cut(s) 1597, 1642
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.