MD10G1197800.v1.1

Release factor glutamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003404
Physical Location & Seq
Forward (+)
29597410 .. 29599184
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1197800.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGAAGCTAAGCTTCGGTCCAACACTCTCACCGTTTCTTAAACCCTCTCTTTTCACAAACCTCCACCGCTCCGTTTGCTCTGCTTCTTCAATACCACCACCACCCACGACCCCAAAACCCCAAACCCCTCTGTTTCTGCGACCGCCCGCCCACTCCGCCACCACCTCCGACCTCCAAAAATGGCACGACTGGGCCAACACCCTCGTTTCCTCAGTTGGGTCAACGTTCGTGGACTTGGACAACGGTCCGGACTCGACCCTTTTGCGCAGAGAGCTCAAATGGCTCATGGAGGACGCAATCGAAGAACCCAAAAACATCGATAACAATGACGGAAGCGTTAGGCTAAGGGTGGAAATTGAGGAGCTTTATATGTTGTGGAAGCAGAGGGTTGAGGACAGGAGGCCCTTTCAGTATGTTGTCGGGTGCGAGCATTGGAGGGACTTGGTGTTGTGTGTTGAAGAAGGGGTTTTGATTCCGAGGCCAGAGACTGAGCTCATTGTTGATTTGGTGGGGGATGTGGTTTTGGGGAATGAGGGGTTGAGGGAGGGTTTGTGGGCTGATTTGGGGACGGGGAGTGGTGCGATTGCGATTGGGATTGCAAGGGTTTTGGGGAGTGGTGGGAGAGTCATTGCTACCGATTTGAGTCCCACGGCAATTGGGGTTGCAGCTTTTAATGTGCAGAGGTATGGTTTGCAGGATGTAGTGGAGCTAAGGCAAGGATCTTGGTTTGAACCATTAAAGGATGTGGAAGGCAAACTCTCAGGTTTTGTAAGTAATCCACCCTACATACCAAGTGACAATATCGCAGGGCTACAAGCTGAAGTTGGACGACATGAACCAAGAGTTGCATTGGACGGCGGTGCCAATGGCATGGATGATCTTTTACATCTTTGCAAAGGGGCTGCTGTTATGTTGAAACCTGGTGGATTCTTTGCTTTCGAGACAAATGGCGAGGAGCAGTGCAAGTATCTCGTAGAGTACATGGAAAACGATGCTGGAGGCAGCTTTTGTAATTTGAACATGGTTCCCGATTTTGCTGGTATTAATAGATTTGTTACTGGATTCCGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MTS Methyltransf_31 PrmA

Protein Analysis

358

Amino Acids

39.18

Weight (kDa)

4.95

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 187 - 247 5.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 266
AccB1I GGYRCC 1 cut(s) 860
AccBSI CCGCTC 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 247
AciI CCGC 5 cut(s) 67, 143, 147, 156, 858
AclI AACGTT 1 cut(s) 224
AclWI GGATC 1 cut(s) 727
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 1 cut(s) 980
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 5 cut(s) 90, 458, 731, 916, 1018
AjnI CCWGG 1 cut(s) 919
AluBI AGCT 9 cut(s) 7, 12, 274, 364, 493, 668, 709, 818, 1005
AluI AGCT 9 cut(s) 7, 12, 274, 364, 493, 668, 709, 818, 1005
Alw21I GWGCWC 2 cut(s) 276, 495
Alw26I GTCTC 2 cut(s) 479, 935
AlwI GGATC 1 cut(s) 727
AlwNI CAGNNNCTG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 3 cut(s) 192, 401, 479
ApeKI GCWGC 3 cut(s) 665, 902, 1002
AseI ATTAAT 1 cut(s) 1044
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 4 cut(s) 17, 192, 245, 402
AsuHPI GGTGA 1 cut(s) 21
AvaII GGWCC 2 cut(s) 17, 245
BanI GGYRCC 1 cut(s) 860
BanII GRGCYC 2 cut(s) 276, 495
Bbv12I GWGCWC 2 cut(s) 276, 495
BbvI GCAGC 3 cut(s) 677, 889, 1014
BceAI ACGGC 2 cut(s) 666, 871
BcgI CGANNNNNNTGC 4 cut(s) 244, 278, 952, 986
BciT130I CCWGG 1 cut(s) 921
BcoDI GTCTC 2 cut(s) 479, 935
BisI GCNGC 3 cut(s) 666, 903, 1003
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 3 cut(s) 667, 904, 1004
Bme1390I CCNGG 1 cut(s) 921
Bme18I GGWCC 2 cut(s) 17, 245
BmgT120I GGNCC 4 cut(s) 17, 192, 245, 402
BmiI GGNNCC 2 cut(s) 862, 1026
BmrFI CCNGG 1 cut(s) 921
BmrI ACTGGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 982
BmuI ACTGGG 1 cut(s) 199
BoxI GACNNNNGTC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 2 cut(s) 344, 710
Bpu1102I GCTNAGC 1 cut(s) 8
Bsa29I ATCGAT 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 476, 648
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 194, 1063
Bse3DI GCAATG 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 1 cut(s) 921
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 476, 648
BseGI GGATG 4 cut(s) 520, 703, 748, 880
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 3 cut(s) 225, 480, 774
BseNI ACTGG 2 cut(s) 194, 1063
BseRI GAGGAG 2 cut(s) 374, 968
BseXI GCAGC 3 cut(s) 677, 889, 1014
BsgI GTGCAG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 3 cut(s) 194, 403, 481
BshNI GGYRCC 1 cut(s) 860
BshVI ATCGAT 1 cut(s) 318
BsiEI CGRYCG 1 cut(s) 143
BsiHKAI GWGCWC 2 cut(s) 276, 495
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 3 cut(s) 452, 580, 630
BslI CCNNNNNNNGG 1 cut(s) 216
BsmAI GTCTC 2 cut(s) 479, 935
BsmFI GGGAC 3 cut(s) 452, 580, 630
BsnI GGCC 3 cut(s) 194, 403, 481
Bsp1286I GDGCHC 2 cut(s) 276, 495
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 2 cut(s) 719, 877
Bsp1720I GCTNAGC 1 cut(s) 8
BspACI CCGC 5 cut(s) 67, 143, 147, 156, 858
BspANI GGCC 3 cut(s) 194, 403, 481
BspCNI CTCAG 3 cut(s) 224, 481, 773
BspDI ATCGAT 1 cut(s) 318
BspEI TCCGGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 862, 1026
BspPI GGATC 1 cut(s) 727
BspT107I GGYRCC 1 cut(s) 860
BsrBI CCGCTC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 627
BsrI ACTGG 2 cut(s) 194, 1063
BssECI CCNNGG 2 cut(s) 476, 648
BssMI GATC 2 cut(s) 719, 877
Bst2UI CCWGG 1 cut(s) 921
Bst4CI ACNGT 2 cut(s) 33, 245
BstC8I GCNNGC 2 cut(s) 147, 428
BstDEI CTNAG 6 cut(s) 8, 211, 344, 489, 710, 760
BstDSI CCRYGG 1 cut(s) 648
BstF5I GGATG 4 cut(s) 520, 703, 748, 880
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 2 cut(s) 722, 880
BstMAI GTCTC 2 cut(s) 479, 935
BstMBI GATC 2 cut(s) 719, 877
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 4 cut(s) 75, 155, 271, 280
BstNI CCWGG 1 cut(s) 921
BstPAI GACNNNNGTC 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 919
BstV1I GCAGC 3 cut(s) 677, 889, 1014
BstX2I RGATCY 1 cut(s) 719
BstXI CCANNNNNNTGG 1 cut(s) 871
BstYI RGATCY 1 cut(s) 719
Bsu15I ATCGAT 1 cut(s) 318
BsuRI GGCC 3 cut(s) 194, 403, 481
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 4 cut(s) 520, 703, 748, 880
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 1 cut(s) 965
Cac8I GCNNGC 2 cut(s) 147, 428
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 4 cut(s) 17, 192, 245, 402
ClaI ATCGAT 1 cut(s) 318
CpoI CGGWCCG 1 cut(s) 245
CseI GACGC 1 cut(s) 302
CsiI ACCWGGT 1 cut(s) 919
Csp6I GTAC 1 cut(s) 979
CspCI CAANNNNNGTGG 4 cut(s) 210, 245, 637, 672
CspI CGGWCCG 1 cut(s) 245
CviAII CATG 5 cut(s) 286, 833, 871, 982, 1021
CviQI GTAC 1 cut(s) 979
DdeI CTNAG 6 cut(s) 8, 211, 344, 489, 710, 760
DpnI GATC 2 cut(s) 721, 879
DpnII GATC 2 cut(s) 719, 877
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 2 cut(s) 274, 493
Eco24I GRGCYC 2 cut(s) 276, 495
Eco47I GGWCC 2 cut(s) 17, 245
Eco53kI GAGCTC 2 cut(s) 274, 493
Eco57I CTGAAG 1 cut(s) 840
EcoICRI GAGCTC 2 cut(s) 274, 493
EcoO109I RGGNCCY 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 919
EcoT38I GRGCYC 2 cut(s) 276, 495
FaeI CATG 5 cut(s) 289, 836, 874, 985, 1024
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 3 cut(s) 452, 580, 630
FatI CATG 5 cut(s) 285, 832, 870, 981, 1020
FauI CCCGC 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 666, 903, 1003
FokI GGATG 4 cut(s) 527, 710, 755, 887
FriOI GRGCYC 2 cut(s) 276, 495
Fsp4HI GCNGC 3 cut(s) 666, 903, 1003
FspI TGCGCA 1 cut(s) 266
GlaI GCGC 1 cut(s) 266
GluI GCNGC 3 cut(s) 666, 903, 1003
GsuI CTGGAG 1 cut(s) 1017
HaeIII GGCC 3 cut(s) 194, 403, 481
HapII CCGG 1 cut(s) 248
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 267
Hin1II CATG 5 cut(s) 289, 836, 874, 985, 1024
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 6 cut(s) 251, 472, 624, 643, 927, 1062
HpaII CCGG 1 cut(s) 248
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 2 cut(s) 222, 232
Hpy188I TCNGA 3 cut(s) 169, 477, 1067
Hpy188III TCNNGA 3 cut(s) 248, 940, 1028
Hpy8I GTNNAC 2 cut(s) 222, 232
HpyAV CCTTC 3 cut(s) 455, 743, 1062
HpyCH4III ACNGT 2 cut(s) 33, 245
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 7 cut(s) 599, 665, 679, 694, 848, 894, 963
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 155, 271, 280
HpyF3I CTNAG 6 cut(s) 8, 211, 344, 489, 710, 760
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 5 cut(s) 289, 836, 874, 985, 1024
HspAI GCGC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 719, 877
LmnI GCTCC 4 cut(s) 74, 361, 706, 955
Lsp1109I GCAGC 3 cut(s) 677, 889, 1014
LweI GCATC 1 cut(s) 982
MabI ACCWGGT 1 cut(s) 919
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 2 cut(s) 794, 1054
MalI GATC 2 cut(s) 721, 879
MbiI CCGCTC 1 cut(s) 69
MboI GATC 2 cut(s) 719, 877
MboII GAAGA 3 cut(s) 78, 314, 470
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 2 cut(s) 276, 495
MluCI AATT 3 cut(s) 354, 654, 1012
MlyI GAGTC 3 cut(s) 245, 633, 652
MmeI TCCRAC 3 cut(s) 44, 192, 805
MroI TCCGGA 1 cut(s) 247
MseI TTAA 4 cut(s) 39, 672, 737, 1044
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 921
MunI CAATTG 1 cut(s) 654
MvaI CCWGG 1 cut(s) 921
MwoI GCNNNNNNNGC 4 cut(s) 75, 155, 271, 280
NdeII GATC 2 cut(s) 719, 877
NlaIII CATG 5 cut(s) 289, 836, 874, 985, 1024
NlaIV GGNNCC 2 cut(s) 862, 1026
NmuCI GTSAC 1 cut(s) 794
NsbI TGCGCA 1 cut(s) 266
PfeI GAWTC 3 cut(s) 472, 927, 1062
PkrI GCNGC 3 cut(s) 667, 904, 1004
PleI GAGTC 3 cut(s) 245, 632, 651
PpsI GAGTC 3 cut(s) 245, 632, 651
PshAI GACNNNNGTC 1 cut(s) 243
PshBI ATTAAT 1 cut(s) 1044
Psp124BI GAGCTC 2 cut(s) 276, 495
Psp1406I AACGTT 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 919
PspGI CCWGG 1 cut(s) 919
PspN4I GGNNCC 2 cut(s) 862, 1026
PspPI GGNCC 4 cut(s) 17, 192, 245, 402
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 1 cut(s) 719
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
Rsr2I CGGWCCG 1 cut(s) 245
RsrII CGGWCCG 1 cut(s) 245
SacI GAGCTC 2 cut(s) 276, 495
SaqAI TTAA 4 cut(s) 39, 672, 737, 1044
SatI GCNGC 3 cut(s) 666, 903, 1003
Sau3AI GATC 2 cut(s) 719, 877
Sau96I GGNCC 4 cut(s) 17, 192, 245, 402
SchI GAGTC 3 cut(s) 245, 633, 652
ScrFI CCNGG 1 cut(s) 921
SduI GDGCHC 2 cut(s) 276, 495
SexAI ACCWGGT 1 cut(s) 919
SfaNI GCATC 1 cut(s) 982
SinI GGWCC 2 cut(s) 17, 245
Sse9I AATT 3 cut(s) 354, 654, 1012
SsiI CCGC 5 cut(s) 67, 143, 147, 156, 858
SstI GAGCTC 2 cut(s) 276, 495
StyD4I CCNGG 1 cut(s) 919
TaaI ACNGT 2 cut(s) 33, 245
TaiI ACGT 1 cut(s) 227
TaqI TCGA 4 cut(s) 254, 300, 318, 939
TaqII GACCGA 1 cut(s) 5
TasI AATT 3 cut(s) 354, 654, 1012
TatI WGTACW 1 cut(s) 978
TfiI GAWTC 3 cut(s) 472, 927, 1062
Tru1I TTAA 4 cut(s) 39, 672, 737, 1044
Tru9I TTAA 4 cut(s) 39, 672, 737, 1044
TscAI CASTG 1 cut(s) 965
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 3 cut(s) 665, 902, 1002
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 2 cut(s) 17, 849
TspGWI ACGGA 2 cut(s) 61, 345
TspRI CASTG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 17, 245
VspI ATTAAT 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.