MD05G1333300.v1.1
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002557
Physical Location & Seq
Reverse (-)
45664080 .. 45668966
4887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1333300.v1.1.491

Sequence Viewer

Length: 2628 bp
ATGGCTAGGGTATTAAGGATAGTTGTTTATCTTGATTTTGTTGTAATTAGTCATTGGATAGTTAGTGATTTATGTTCCATGGCGTATCCTTCGTCGGAATCGGCTACACATGTTCATCCCATTCCGACTTCTTCCTCAAACCCTAATTCTTCTCTTTCTACCTCCATTATGATCCAAAACATCGGATCTATGGTGCCAATCAAGCTCACCACTACAAATTACTTGACCTGGAGTGCTCTCTTTGCCCCAATCTTCCGTCGCTACAATCTTACCGGTCTCATTGATGGATCTATGGCGGCACCGCCTCAATTTCTTCTTGATTCTTCCGGTAATCGCAATTTCAACCCTGACTATGTTTCTTGGTATGCGAATGATCAAAATATTCTCATATGGATCAACTCTACACTTTCTGAATCCCTAATTCCCTATACTATGGGTGTAAATTCCTCTCGAGAGCTATGGTCTAAGCTTGAATCGTGCCTTGCAGCAGCTTCTCAATCACATATTCATGAACTCCGATCTCGTCTTCATAATATTTCAAAAGGTGATTCCACCGCCTTTGCTTATCTTCAACAAATTGAAGACATTGCTGATGCTCTTGCCAGCGCCGAAGCTCCGACTGAAGACTCTGATCTGATTTCTATAACTCTTCATAGCCTGCCGCCGGAGTATGATTCCTTTGTTGATGCGATCCAATTCCGTCTTGGCTCTACAACACTTGATGAGTTACATGGCTTACTTCTAAGTAAAGAAATTCAGCTTACCACTCGCAAGAAAGCTACTACATCCTCTGGCGTTTTTCAGGCCTCAATTCGAATCTTGCATTATCCTCTGGCATTCTTCCTACACCGACTTCCAATACTGGCTCTCAGGCCTTTCTTGCTCAGAATTCATCTTAGTTTAATCAGTATCAAGGTTGTTCTCAAGCTCGCATTTTGTCTAACCCTAGGAATTCTCAAAATAAGGGCAATTTCTCCGGTTATTGTGGTAACAGTCAGCGTCCAAATTACTCTCGCTATACTCAGAGGAACAATTGTGGAGGTCGCTCGGGCTTTAATTTCAGTGGCAGAATATCAACTTGTCAAATATGTCATGGCTATGTGTGCTAATCCTTCACAGAGTACTTCTCATTCCTGGCTTCTTGATTCAGAAGCTAGCTCACATATGACAAGTTCTCTGGCGAATCTTCAAAATCCAGCACCATACACCGGCCCTGAACAAGTCTACATTGGTGAAGGTTTGCCTATATCTAATTCTGGTTCTACCTCTATATCTACTCCTCATCATCGTTTTGATTTGCATGATGTGTTACATGTGCCTGATTTGAAGCAAGATTTACTCTCTGCAAATAAATTTACACTTGATAATTGGTGTTCAATTCGCCTATATCATTTTCACTTCGCTGTGAAGGATCATATTTCGAAGAGGACGCTTTTTAAAGGACCTGTGCAAAATGGATTTTATCCATTCATTACATCTGTCAATGATGGTTCACATAAGATTTTTGCAGCTACTATCAAAGCTTCTCCAGAGATTTGGCGTCAAAGATTGGGTCATCTCTCTTTCAAAGTTTTACATAAACTTGTGTCTAAGTCTAGTATTCCTCTCACTACTATTATGAATAAAACCTTCTGTTCAAATTGTGCATTGGGTAAAAGTTCAAAACTGCCATTTGTATCAGCTTCTAGTGTTACCACTAGACCTTTGGAACTTTTACATGCAGATGTTTGGGGTCCAGCTCCATTTCTCTCTGTATCTGGTTATCGGTTTTATCTTTTTATAGTGGATGATTTCAGTCGATATAACTGGTTCTTTCCGATCAAATGTAAATCTGAGGTTGAAGCATTCTCCACAGCATTTTATCTCATAAACAGGTTGCCTACTATGTCTAGATCCTGTCCTTGGGAACAACTTTTCAGCAAGCCTCCAGATTATACAACACGTAAAGTGTTTGGGTGCAGGTGTTTTCCTTGGCTAAAACCTTATGTAGCTTCCAAACTTGAACCCAAAAGCAAGCCCTGTGTCTTTCTTGGCTTTAGTCTCAATCACAATGGCTATAGATGTCTTGATCTTCAATCCAACAAGATTTATATTTCAAGGCATGTCATCTTTGATGAGCTGACTTTCCCATTTCATCAAGTTTCTCCAGTTTCATCTTCTTCATCCTCAGCCTCAATCTTTTCCTCTCCCATACCTTCAACTCTTACCTTCACCAAATTCCCTGCAGCATCTTATCCTATGCAACTTGTAATCAACCTAAATCAATATGCACCTTTTTCTACTTCAACTTTTTCACCTCCTCTCCCTCATTCTCCTCACCCTGTCTCTCCTCATTCCTCACACATATCACCAACCCCACCTTTAAACACTCATCCTATGCAAACAAGATCAAAATCCAACATTTTTAAACCAAAAGCCTATTCAGCTACCAAACACCCTCTTCCTTCCCAAATTTCTGCAGATAATCTTCCTGCTACTTATCTCCAAGCTTCAAAACATCCTCACTGGAGACAAGATATGCAAGAGGAATTTAATGTCCTGGTTCACATAGGCACTTGGGCGCGTCCAGTTCCAGCAATAGGGGAATTATGGGGTTTTCTCTGTTTGCAACCTAGACAACAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

876

Amino Acids

97.85

Weight (kDa)

9.13

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 117 - 254 2.1e-12 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 379 - 453 7.2e-10 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 497 - 553 1.3e-11 GAG-pre-integrase domain
SH3_retrovirus PF25597 653 - 714 1.2e-22 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1950
Acc36I ACCTGC 1 cut(s) 1950
AccB1I GGYRCC 2 cut(s) 193, 298
AccI GTMKAC 1 cut(s) 1224
AccII CGCG 1 cut(s) 2563
AciI CCGC 4 cut(s) 296, 302, 555, 662
AclWI GGATC 7 cut(s) 166, 193, 295, 401, 685, 1419, 1887
AcsI RAATTY 8 cut(s) 442, 753, 888, 951, 1352, 2216, 2451, 2528
AcuI CTGAAG 1 cut(s) 642
AcyI GRCGYC 1 cut(s) 1540
AfaI GTAC 1 cut(s) 1123
AfiI CCNNNNNNNGG 4 cut(s) 664, 1208, 1902, 2579
AflIII ACRYGT 3 cut(s) 109, 1312, 1940
AgeI ACCGGT 1 cut(s) 272
AjnI CCWGG 3 cut(s) 227, 1133, 2538
AjuI GAANNNNNNNTTGG 2 cut(s) 2404, 2436
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 4 cut(s) 281, 2045, 2329, 2503
AlwI GGATC 7 cut(s) 166, 193, 295, 401, 685, 1419, 1887
Ama87I CYCGRG 2 cut(s) 450, 1047
AoxI GGCC 3 cut(s) 804, 872, 1210
ApeKI GCWGC 4 cut(s) 485, 488, 1508, 2225
ApoI RAATTY 8 cut(s) 442, 753, 888, 951, 1352, 2216, 2451, 2528
ArsI GACNNNNNNTTYG 3 cut(s) 1537, 1569, 2607
AsiGI ACCGGT 1 cut(s) 272
AspA2I CCTAGG 1 cut(s) 946
AspLEI GCGC 2 cut(s) 608, 2563
AspS9I GGNCC 3 cut(s) 1211, 1442, 1733
AsuHPI GGTGA 7 cut(s) 199, 557, 1244, 2203, 2286, 2309, 2340
AsuII TTCGAA 2 cut(s) 814, 1421
AsuNHI GCTAGC 1 cut(s) 1154
AvaI CYCGRG 2 cut(s) 450, 1047
AvaII GGWCC 2 cut(s) 1442, 1733
AvrII CCTAGG 1 cut(s) 946
BanI GGYRCC 2 cut(s) 193, 298
BbsI GAAGAC 3 cut(s) 518, 588, 630
Bbv12I GWGCWC 1 cut(s) 238
BbvCI CCTCAGC 1 cut(s) 2167
BbvI GCAGC 4 cut(s) 497, 500, 1520, 2237
BccI CCATC 2 cut(s) 278, 1481
BciT130I CCWGG 3 cut(s) 229, 1135, 2540
BciVI GTATCC 1 cut(s) 96
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 4 cut(s) 281, 2045, 2329, 2503
BfaI CTAG 8 cut(s) 6, 947, 1155, 1596, 1686, 1698, 1890, 2613
BfmI CTRYAG 3 cut(s) 2056, 2223, 2457
BfoI RGCGCY 1 cut(s) 609
BfuAI ACCTGC 1 cut(s) 1950
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 6 cut(s) 297, 486, 489, 662, 1509, 2226
BlnI CCTAGG 1 cut(s) 946
BlsI GCNGC 6 cut(s) 298, 487, 490, 663, 1510, 2227
BmcAI AGTACT 1 cut(s) 1123
Bme1390I CCNGG 3 cut(s) 229, 1135, 2540
Bme18I GGWCC 2 cut(s) 1442, 1733
BmeT110I CYCGRG 2 cut(s) 450, 1047
BmgT120I GGNCC 3 cut(s) 1211, 1442, 1733
BmiI GGNNCC 3 cut(s) 195, 300, 1734
BmrFI CCNGG 3 cut(s) 229, 1135, 2540
BmsI GCATC 3 cut(s) 583, 676, 2237
BmtI GCTAGC 1 cut(s) 1158
BpiI GAAGAC 3 cut(s) 518, 588, 630
BplI GAGNNNNNCTC 2 cut(s) 1111, 1143
BpmI CTGGAG 5 cut(s) 250, 1512, 1911, 2130, 2527
Bpu10I CCTNAGC 1 cut(s) 2167
Bpu14I TTCGAA 2 cut(s) 814, 1421
BpuEI CTTGAG 1 cut(s) 908
BsaAI YACGTR 1 cut(s) 1943
BsaBI GATNNNNATC 1 cut(s) 2392
BsaHI GRCGYC 1 cut(s) 1540
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 4 cut(s) 78, 946, 1901, 1970
BsaWI WCCGGW 3 cut(s) 272, 326, 976
BsaXI ACNNNNNCTCC 2 cut(s) 2286, 2316
Bsc4I CCNNNNNNNGG 4 cut(s) 664, 1208, 1902, 2579
Bse118I RCCGGY 2 cut(s) 272, 1208
Bse1I ACTGG 5 cut(s) 867, 1811, 2147, 2510, 2567
Bse3DI GCAATG 1 cut(s) 585
Bse8I GATNNNNATC 1 cut(s) 2392
BseBI CCWGG 3 cut(s) 229, 1135, 2540
BseDI CCNNGG 4 cut(s) 78, 946, 1901, 1970
BseGI GGATG 6 cut(s) 115, 785, 1792, 2162, 2371, 2497
BseJI GATNNNNATC 1 cut(s) 2392
BseLI CCNNNNNNNGG 4 cut(s) 664, 1208, 1902, 2579
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 5 cut(s) 883, 898, 1036, 1824, 2181
BseNI ACTGG 5 cut(s) 867, 1811, 2147, 2510, 2567
BseRI GAGGAG 4 cut(s) 1269, 2289, 2304, 2319
BseXI GCAGC 4 cut(s) 497, 500, 1520, 2237
BsgI GTGCAG 1 cut(s) 1978
Bsh1236I CGCG 1 cut(s) 2563
BshFI GGCC 3 cut(s) 806, 874, 1212
BshNI GGYRCC 2 cut(s) 193, 298
BshTI ACCGGT 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 2 cut(s) 450, 1047
BsiSI CCGG 5 cut(s) 273, 327, 665, 977, 1209
BslI CCNNNNNNNGG 4 cut(s) 664, 1208, 1902, 2579
BsmAI GTCTC 4 cut(s) 281, 2045, 2329, 2503
BsmI GAATGC 2 cut(s) 836, 1844
BsnI GGCC 3 cut(s) 806, 874, 1212
Bso31I GGTCTC 1 cut(s) 281
BsoBI CYCGRG 2 cut(s) 450, 1047
Bsp119I TTCGAA 2 cut(s) 814, 1421
Bsp1286I GDGCHC 1 cut(s) 238
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 4 cut(s) 296, 302, 555, 662
BspANI GGCC 3 cut(s) 806, 874, 1212
BspCNI CTCAG 5 cut(s) 882, 897, 1035, 1825, 2180
BspFNI CGCG 1 cut(s) 2563
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 195, 300, 1734
BspMAI CTGCAG 2 cut(s) 2227, 2461
BspMI ACCTGC 1 cut(s) 1950
BspOI GCTAGC 1 cut(s) 1158
BspPI GGATC 7 cut(s) 166, 193, 295, 401, 685, 1419, 1887
BspT104I TTCGAA 2 cut(s) 814, 1421
BspT107I GGYRCC 2 cut(s) 193, 298
BspTNI GGTCTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 585
BsrFI RCCGGY 2 cut(s) 272, 1208
BsrI ACTGG 5 cut(s) 867, 1811, 2147, 2510, 2567
BssAI RCCGGY 2 cut(s) 272, 1208
BssECI CCNNGG 4 cut(s) 78, 946, 1901, 1970
BssNI GRCGYC 1 cut(s) 1540
BssT1I CCWWGG 4 cut(s) 78, 946, 1901, 1970
Bst2UI CCWGG 3 cut(s) 229, 1135, 2540
Bst4CI ACNGT 2 cut(s) 994, 2622
Bst6I CTCTTC 3 cut(s) 654, 1418, 2445
BstACI GRCGYC 1 cut(s) 1540
BstBAI YACGTR 1 cut(s) 1943
BstBI TTCGAA 2 cut(s) 814, 1421
BstC8I GCNNGC 6 cut(s) 604, 659, 930, 1156, 1922, 2015
BstDEI CTNAG 9 cut(s) 465, 743, 869, 884, 896, 1022, 1590, 1833, 2167
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 6 cut(s) 115, 785, 1792, 2162, 2371, 2497
BstFNI CGCG 1 cut(s) 2563
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 2 cut(s) 608, 2563
BstMAI GTCTC 4 cut(s) 281, 2045, 2329, 2503
BstMWI GCNNNNNNNGC 5 cut(s) 202, 242, 880, 1103, 2423
BstNI CCWGG 3 cut(s) 229, 1135, 2540
BstNSI RCATGY 4 cut(s) 113, 1316, 1721, 2105
BstSCI CCNGG 3 cut(s) 227, 1133, 2538
BstSFI CTRYAG 3 cut(s) 2056, 2223, 2457
BstUI CGCG 1 cut(s) 2563
BstV1I GCAGC 4 cut(s) 497, 500, 1520, 2237
BstV2I GAAGAC 3 cut(s) 518, 588, 630
BstX2I RGATCY 3 cut(s) 185, 287, 1892
BstXI CCANNNNNNTGG 1 cut(s) 1536
BstYI RGATCY 3 cut(s) 185, 287, 1892
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 3 cut(s) 806, 874, 1212
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 6 cut(s) 115, 785, 1792, 2162, 2371, 2497
BtsIMutI CAGTG 2 cut(s) 1068, 2503
BveI ACCTGC 1 cut(s) 1950
Cac8I GCNNGC 6 cut(s) 604, 659, 930, 1156, 1922, 2015
CciI TCATGA 1 cut(s) 508
CfoI GCGC 2 cut(s) 608, 2563
Cfr10I RCCGGY 2 cut(s) 272, 1208
Cfr13I GGNCC 3 cut(s) 1211, 1442, 1733
CseI GACGC 4 cut(s) 988, 1438, 1529, 2552
Csp6I GTAC 1 cut(s) 1122
CspAI ACCGGT 1 cut(s) 272
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviAII CATG 9 cut(s) 79, 110, 509, 731, 1093, 1301, 1313, 1718, 2102
CviQI GTAC 1 cut(s) 1122
DdeI CTNAG 9 cut(s) 465, 743, 869, 884, 896, 1022, 1590, 1833, 2167
DraI TTTAAA 3 cut(s) 1438, 2364, 2407
Eam1104I CTCTTC 3 cut(s) 654, 1418, 2445
EarI CTCTTC 3 cut(s) 654, 1418, 2445
Eco130I CCWWGG 4 cut(s) 78, 946, 1901, 1970
Eco147I AGGCCT 2 cut(s) 806, 874
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 2 cut(s) 1442, 1733
Eco57I CTGAAG 1 cut(s) 642
Eco88I CYCGRG 2 cut(s) 450, 1047
EcoO109I RGGNCCY 1 cut(s) 1442
EcoRI GAATTC 2 cut(s) 888, 951
EcoRII CCWGG 3 cut(s) 227, 1133, 2538
EcoT14I CCWWGG 4 cut(s) 78, 946, 1901, 1970
ErhI CCWWGG 4 cut(s) 78, 946, 1901, 1970
FaeI CATG 9 cut(s) 82, 113, 512, 734, 1096, 1304, 1316, 1721, 2105
FatI CATG 9 cut(s) 78, 109, 508, 730, 1092, 1300, 1312, 1717, 2101
FauNDI CATATG 2 cut(s) 389, 1164
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 1224
Fnu4HI GCNGC 6 cut(s) 297, 486, 489, 662, 1509, 2226
FokI GGATG 6 cut(s) 102, 772, 1799, 2149, 2358, 2484
Fsp4HI GCNGC 6 cut(s) 297, 486, 489, 662, 1509, 2226
FspBI CTAG 8 cut(s) 6, 947, 1155, 1596, 1686, 1698, 1890, 2613
GlaI GCGC 2 cut(s) 607, 2562
GluI GCNGC 6 cut(s) 297, 486, 489, 662, 1509, 2226
GsuI CTGGAG 5 cut(s) 250, 1512, 1911, 2130, 2527
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 3 cut(s) 806, 874, 1212
HapII CCGG 5 cut(s) 273, 327, 665, 977, 1209
HgaI GACGC 4 cut(s) 988, 1438, 1529, 2552
HhaI GCGC 2 cut(s) 608, 2563
Hin1I GRCGYC 1 cut(s) 1540
Hin1II CATG 9 cut(s) 82, 113, 512, 734, 1096, 1304, 1316, 1721, 2105
Hin6I GCGC 2 cut(s) 606, 2561
HinP1I GCGC 2 cut(s) 606, 2561
HindIII AAGCTT 3 cut(s) 467, 1521, 2487
HpaII CCGG 5 cut(s) 273, 327, 665, 977, 1209
HphI GGTGA 7 cut(s) 199, 557, 1244, 2203, 2286, 2309, 2340
Hpy166II GTNNAC 3 cut(s) 1225, 1493, 2545
Hpy8I GTNNAC 3 cut(s) 1225, 1493, 2545
Hpy99I CGWCG 2 cut(s) 97, 261
HpyAV CCTTC 8 cut(s) 99, 1122, 1229, 1402, 1639, 2205, 2218, 2454
HpyCH4III ACNGT 2 cut(s) 994, 2622
HpyCH4IV ACGT 1 cut(s) 1942
HpyF10VI GCNNNNNNNGC 5 cut(s) 202, 242, 880, 1103, 2423
HpyF3I CTNAG 9 cut(s) 465, 743, 869, 884, 896, 1022, 1590, 1833, 2167
HpySE526I ACGT 1 cut(s) 1942
Hsp92I GRCGYC 1 cut(s) 1540
Hsp92II CATG 9 cut(s) 82, 113, 512, 734, 1096, 1304, 1316, 1721, 2105
HspAI GCGC 2 cut(s) 606, 2561
Ksp22I TGATCA 1 cut(s) 373
LmnI GCTCC 2 cut(s) 619, 1744
Lsp1109I GCAGC 4 cut(s) 497, 500, 1520, 2237
LweI GCATC 3 cut(s) 583, 676, 2237
MaeI CTAG 8 cut(s) 6, 947, 1155, 1596, 1686, 1698, 1890, 2613
MaeII ACGT 1 cut(s) 1942
MaeIII GTNAC 4 cut(s) 726, 988, 1308, 1690
MfeI CAATTG 1 cut(s) 1032
MflI RGATCY 3 cut(s) 185, 287, 1892
MhlI GDGCHC 1 cut(s) 238
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 5 cut(s) 75, 149, 641, 2103, 2421
MseI TTAA 7 cut(s) 14, 902, 1055, 1437, 2363, 2406, 2532
MslI CAYNNNNRTG 3 cut(s) 507, 1097, 1722
MspI CCGG 5 cut(s) 273, 327, 665, 977, 1209
MspR9I CCNGG 3 cut(s) 229, 1135, 2540
MunI CAATTG 1 cut(s) 1032
Mva1269I GAATGC 2 cut(s) 836, 1844
MvaI CCWGG 3 cut(s) 229, 1135, 2540
MvnI CGCG 1 cut(s) 2563
MwoI GCNNNNNNNGC 5 cut(s) 202, 242, 880, 1103, 2423
NcoI CCATGG 1 cut(s) 78
NdeI CATATG 2 cut(s) 389, 1164
NheI GCTAGC 1 cut(s) 1154
NlaIII CATG 9 cut(s) 82, 113, 512, 734, 1096, 1304, 1316, 1721, 2105
NlaIV GGNNCC 3 cut(s) 195, 300, 1734
NspI RCATGY 4 cut(s) 113, 1316, 1721, 2105
NspV TTCGAA 2 cut(s) 814, 1421
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 1 cut(s) 508
PaqCI CACCTGC 1 cut(s) 1950
PceI AGGCCT 2 cut(s) 806, 874
PciI ACATGT 2 cut(s) 109, 1312
PctI GAATGC 2 cut(s) 836, 1844
PfeI GAWTC 9 cut(s) 98, 320, 413, 473, 548, 674, 816, 1145, 1183
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 6 cut(s) 298, 487, 490, 663, 1510, 2227
PleI GAGTC 1 cut(s) 620
PpsI GAGTC 1 cut(s) 620
Ppu21I YACGTR 1 cut(s) 1943
PpuMI RGGWCCY 1 cut(s) 1442
PscI ACATGT 2 cut(s) 109, 1312
Psp5II RGGWCCY 1 cut(s) 1442
Psp6I CCWGG 3 cut(s) 227, 1133, 2538
PspGI CCWGG 3 cut(s) 227, 1133, 2538
PspN4I GGNNCC 3 cut(s) 195, 300, 1734
PspPI GGNCC 3 cut(s) 1211, 1442, 1733
PspPPI RGGWCCY 1 cut(s) 1442
PstI CTGCAG 2 cut(s) 2227, 2461
PsuI RGATCY 3 cut(s) 185, 287, 1892
RsaI GTAC 1 cut(s) 1123
RsaNI GTAC 1 cut(s) 1122
RseI CAYNNNNRTG 3 cut(s) 507, 1097, 1722
SaqAI TTAA 7 cut(s) 14, 902, 1055, 1437, 2363, 2406, 2532
SatI GCNGC 6 cut(s) 297, 486, 489, 662, 1509, 2226
Sau96I GGNCC 3 cut(s) 1211, 1442, 1733
ScaI AGTACT 1 cut(s) 1123
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 3 cut(s) 229, 1135, 2540
SduI GDGCHC 1 cut(s) 238
SfaNI GCATC 3 cut(s) 583, 676, 2237
SfcI CTRYAG 3 cut(s) 2056, 2223, 2457
Sfr274I CTCGAG 1 cut(s) 450
SfuI TTCGAA 2 cut(s) 814, 1421
SinI GGWCC 2 cut(s) 1442, 1733
SlaI CTCGAG 1 cut(s) 450
SmiMI CAYNNNNRTG 3 cut(s) 507, 1097, 1722
SmlI CTYRAG 2 cut(s) 450, 923
SmoI CTYRAG 2 cut(s) 450, 923
SseBI AGGCCT 2 cut(s) 806, 874
SsiI CCGC 4 cut(s) 296, 302, 555, 662
SspI AATATT 2 cut(s) 382, 535
SspMI CTAG 8 cut(s) 6, 947, 1155, 1596, 1686, 1698, 1890, 2613
StuI AGGCCT 2 cut(s) 806, 874
StyD4I CCNGG 3 cut(s) 227, 1133, 2538
StyI CCWWGG 4 cut(s) 78, 946, 1901, 1970
TaaI ACNGT 2 cut(s) 994, 2622
TaiI ACGT 1 cut(s) 1945
TaqI TCGA 4 cut(s) 451, 814, 1421, 1798
TatI WGTACW 1 cut(s) 1121
TauI GCSGC 2 cut(s) 299, 664
TfiI GAWTC 9 cut(s) 98, 320, 413, 473, 548, 674, 816, 1145, 1183
Tru1I TTAA 7 cut(s) 14, 902, 1055, 1437, 2363, 2406, 2532
Tru9I TTAA 7 cut(s) 14, 902, 1055, 1437, 2363, 2406, 2532
TscAI CASTG 2 cut(s) 1068, 2510
TseI GCWGC 4 cut(s) 485, 488, 1508, 2225
TspGWI ACGGA 2 cut(s) 245, 689
TspRI CASTG 2 cut(s) 1068, 2510
VpaK11BI GGWCC 2 cut(s) 1442, 1733
XapI RAATTY 8 cut(s) 442, 753, 888, 951, 1352, 2216, 2451, 2528
XbaI TCTAGA 1 cut(s) 1889
XceI RCATGY 4 cut(s) 113, 1316, 1721, 2105
XcmI CCANNNNNNNNNTGG 2 cut(s) 701, 1702
XhoI CTCGAG 1 cut(s) 450
XmaJI CCTAGG 1 cut(s) 946
XmiI GTMKAC 1 cut(s) 1224
XspI CTAG 8 cut(s) 6, 947, 1155, 1596, 1686, 1698, 1890, 2613
ZrmI AGTACT 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.