Rroxscaffold_5G00336230
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002557
Physical Location & Seq
Reverse (-)
3973913 .. 3975648
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00336230.1

Sequence Viewer

Length: 654 bp
ATGGCAATGCTTAGAACAATGCTTCAACAAGTATGGTGGAGTGCCAAATTTGTGGTCAAAAGGGGCATGCTGCTCTTGATTGTGTTCACAGAGGTAACTATGCTTACCAGGGAGCTCCTCCTTCACATAAACTTCATGCTATGGCAGCTCAAAGTCTACATGACTTCTCTGATGGTGATGCTTAGATCCTTGATACCGGTGCAATACATCATATATATCACTTCTGATATGAACAACCTCACTAGTGCAAAGGCGCATAATGCCAATGACAAGATTATTGTCGGGAATGGTGATGGTCTACAAATCAAACATATTGGTTCAACTGATATTACTCCTTCATCTCACACTTTACACTTTAAAAATGTGTTGCATGTGCCTCAGATTACTCAAAAGTTGATGTCTGTCAACAAGTTATGTAGAGATAATAAATGCAGGATTATTCTTGATGATGAAATTTTTCTAGTGCAGGACAAGGCAACTTTGGAGGTTCTGTTCAAAGGAAGCAGTAGTAGTAATGGCCTTTACAAAATCCCACATAAGCATGTCTCATTGTCACCATTTGCTTTTCTAGGTCAAAGAATTCAATCCTCTCTATGGCATCAACGGCTAGCCCATCCTACCAATGCCATCGTAATCAAAATGCTTGTAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

217

Amino Acids

24.79

Weight (kDa)

9.88

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 73 - 139 7.5e-08 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 156, 298
AclWI GGATC 1 cut(s) 180
AcsI RAATTY 3 cut(s) 47, 453, 579
AfiI CCNNNNNNNGG 1 cut(s) 594
AgeI ACCGGT 1 cut(s) 196
AgsI TTSAA 4 cut(s) 26, 321, 496, 584
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 1 cut(s) 107
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 2 cut(s) 115, 148
AluI AGCT 2 cut(s) 115, 148
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 550
AlwI GGATC 1 cut(s) 180
AoxI GGCC 1 cut(s) 517
ApeKI GCWGC 2 cut(s) 70, 145
ApoI RAATTY 3 cut(s) 47, 453, 579
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AsiGI ACCGGT 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 456
AspLEI GCGC 1 cut(s) 256
AsuHPI GGTGA 3 cut(s) 187, 302, 546
AsuNHI GCTAGC 1 cut(s) 607
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 57, 157
BccI CCATC 4 cut(s) 166, 287, 621, 635
BceAI ACGGC 1 cut(s) 620
BciT130I CCWGG 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 550
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 5 cut(s) 243, 461, 569, 608, 652
BisI GCNGC 2 cut(s) 71, 146
BlsI GCNGC 2 cut(s) 72, 147
Bme1390I CCNGG 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 2 cut(s) 168, 607
BmtI GCTAGC 1 cut(s) 611
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse118I RCCGGY 1 cut(s) 196
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 392
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 2 cut(s) 57, 157
BsgI GTGCAG 1 cut(s) 485
BshFI GGCC 1 cut(s) 519
BshTI ACCGGT 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 1 cut(s) 550
BsnI GGCC 1 cut(s) 519
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 1 cut(s) 185
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 1 cut(s) 391
BspOI GCTAGC 1 cut(s) 611
BspPI GGATC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 109
BstC8I GCNNGC 2 cut(s) 68, 609
BstDEI CTNAG 3 cut(s) 11, 182, 378
BstF5I GGATG 1 cut(s) 613
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 1 cut(s) 188
BstMAI GTCTC 1 cut(s) 550
BstMBI GATC 1 cut(s) 185
BstMWI GCNNNNNNNGC 3 cut(s) 145, 260, 604
BstNI CCWGG 1 cut(s) 109
BstNSI RCATGY 3 cut(s) 70, 374, 545
BstSCI CCNGG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 57, 157
BstX2I RGATCY 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 1 cut(s) 519
BtsCI GGATG 1 cut(s) 613
Cac8I GCNNGC 2 cut(s) 68, 609
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 196
CspAI ACCGGT 1 cut(s) 196
CspCI CAANNNNNGTGG 2 cut(s) 17, 52
CviAII CATG 5 cut(s) 67, 136, 160, 371, 542
CviJI RGCY 6 cut(s) 115, 148, 519, 607, 611, 650
CviKI_1 RGCY 6 cut(s) 115, 148, 519, 607, 611, 650
DdeI CTNAG 3 cut(s) 11, 182, 378
DpnI GATC 1 cut(s) 187
DpnII GATC 1 cut(s) 185
DraI TTTAAA 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 115
EcoRI GAATTC 1 cut(s) 579
EcoRII CCWGG 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 117
FaeI CATG 5 cut(s) 70, 139, 163, 374, 545
FatI CATG 5 cut(s) 66, 135, 159, 370, 541
FblI GTMKAC 2 cut(s) 156, 298
Fnu4HI GCNGC 2 cut(s) 71, 146
FokI GGATG 1 cut(s) 600
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 2 cut(s) 71, 146
FspBI CTAG 5 cut(s) 243, 461, 569, 608, 652
GlaI GCGC 1 cut(s) 255
GluI GCNGC 2 cut(s) 71, 146
HaeIII GGCC 1 cut(s) 519
HapII CCGG 1 cut(s) 197
HhaI GCGC 1 cut(s) 256
Hin1II CATG 5 cut(s) 70, 139, 163, 374, 545
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 187, 302, 546
Hpy166II GTNNAC 4 cut(s) 87, 157, 299, 406
Hpy188I TCNGA 3 cut(s) 171, 226, 381
Hpy188III TCNNGA 3 cut(s) 76, 283, 443
Hpy8I GTNNAC 4 cut(s) 87, 157, 299, 406
HpyAV CCTTC 2 cut(s) 131, 345
HpyCH4V TGCA 5 cut(s) 202, 248, 370, 432, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 260, 604
HpyF3I CTNAG 3 cut(s) 11, 182, 378
Hsp92II CATG 5 cut(s) 70, 139, 163, 374, 545
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 1 cut(s) 185
LmnI GCTCC 2 cut(s) 112, 120
LpnPI CCDG 5 cut(s) 94, 121, 210, 418, 452
Lsp1109I GCAGC 2 cut(s) 57, 157
LweI GCATC 2 cut(s) 168, 607
MaeI CTAG 5 cut(s) 243, 461, 569, 608, 652
MaeIII GTNAC 2 cut(s) 94, 552
MalI GATC 1 cut(s) 187
MboI GATC 1 cut(s) 185
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 3 cut(s) 47, 453, 579
MnlI CCTC 6 cut(s) 85, 128, 248, 387, 478, 598
MroXI GAANNNNTTC 1 cut(s) 456
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 540
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 3 cut(s) 145, 260, 604
NdeII GATC 1 cut(s) 185
NheI GCTAGC 1 cut(s) 607
NlaIII CATG 5 cut(s) 70, 139, 163, 374, 545
NmuCI GTSAC 1 cut(s) 552
NspI RCATGY 3 cut(s) 70, 374, 545
PaeI GCATGC 1 cut(s) 70
PdmI GAANNNNTTC 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 196
PkrI GCNGC 2 cut(s) 72, 147
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PsuI RGATCY 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 540
SacI GAGCTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 2 cut(s) 71, 146
Sau3AI GATC 1 cut(s) 185
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 1 cut(s) 117
SetI ASST 6 cut(s) 96, 117, 150, 240, 489, 574
SfaNI GCATC 2 cut(s) 168, 607
SmiMI CAYNNNNRTG 1 cut(s) 540
SpeI ACTAGT 1 cut(s) 242
SphI GCATGC 1 cut(s) 70
Sse9I AATT 3 cut(s) 47, 453, 579
SspMI CTAG 5 cut(s) 243, 461, 569, 608, 652
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 107
TasI AATT 3 cut(s) 47, 453, 579
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseFI GTSAC 1 cut(s) 552
TseI GCWGC 2 cut(s) 70, 145
Tsp45I GTSAC 1 cut(s) 552
TspDTI ATGAA 4 cut(s) 124, 245, 327, 465
XapI RAATTY 3 cut(s) 47, 453, 579
XceI RCATGY 3 cut(s) 70, 374, 545
XmiI GTMKAC 2 cut(s) 156, 298
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 5 cut(s) 243, 461, 569, 608, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.