pycom02g12970

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002557
Physical Location & Seq
Reverse (-)
9554364 .. 9554876
513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12970.1

Sequence Viewer

Length: 513 bp
ATGGTGCATGGACTTCCATACTTAGAAGAAGTTGATGAAGTTTGTGAAGGTTGTCAGCTTGGAAAACAGCATAGAGAGTGGTTTCCAAAGAATCAGGCATGGAGAGCAAGCAATCCTCTAGAATTAATTCATGTAGACTTATGTGGGCCTATGCAAAATGAGTCCATTGCTGGTAACAAGTACTTTATGTTTCTTGTAGATGACTGCACAAGAATGATGTGGGTTTATTTTCTTAGATACAAGTCAGATGCAGTGAATTGTTTTAGAAAATTCAAATCTATGGTAAAGCTACATAATGGTTTTAGAGTAAAATGCCTGAGAAGTGACAAAGGGGGTGAATTCACATCCTGTGAGTTTAACAAGTTGTGTGAAGATGAAGGTATTCAAAGACAACTCTCTATGGCCTATACTCCACAACTCCATTTTGTTGTAGAAAGAAAGAACAGAAATGTAGTTGAAATGGCAAAAGCCATGCTTCATGATAAAGGATTACCTTACTATTTGTGGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4219 RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

171

Amino Acids

20.03

Weight (kDa)

8.09

Isoelectric Point (pI)

24.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 38 - 138 1.4e-11 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 135
AcsI RAATTY 2 cut(s) 269, 338
AfaI GTAC 1 cut(s) 182
AgsI TTSAA 3 cut(s) 274, 386, 458
AluBI AGCT 2 cut(s) 58, 289
AluI AGCT 2 cut(s) 58, 289
AoxI GGCC 2 cut(s) 146, 402
ApoI RAATTY 2 cut(s) 269, 338
AseI ATTAAT 1 cut(s) 125
Asp700I GAANNNNTTC 2 cut(s) 126, 381
AspS9I GGNCC 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 347
BfaI CTAG 1 cut(s) 119
BmcAI AGTACT 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 146
BmsI GCATC 1 cut(s) 238
Bse3DI GCAATG 1 cut(s) 165
BseGI GGATG 1 cut(s) 344
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 2 cut(s) 148, 404
BsnI GGCC 2 cut(s) 148, 404
BspANI GGCC 2 cut(s) 148, 404
BspCNI CTCAG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 3 cut(s) 22, 233, 317
BstF5I GGATG 1 cut(s) 344
BstMWI GCNNNNNNNGC 1 cut(s) 104
BsuRI GGCC 2 cut(s) 148, 404
BtsCI GGATG 1 cut(s) 344
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 109
CciI TCATGA 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 146
Csp6I GTAC 1 cut(s) 181
CviAII CATG 5 cut(s) 8, 99, 131, 472, 479
CviJI RGCY 5 cut(s) 58, 148, 289, 404, 470
CviKI_1 RGCY 5 cut(s) 58, 148, 289, 404, 470
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 3 cut(s) 22, 233, 317
EcoRI GAATTC 1 cut(s) 338
FaeI CATG 5 cut(s) 11, 102, 134, 475, 482
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FatI CATG 5 cut(s) 7, 98, 130, 471, 478
FblI GTMKAC 1 cut(s) 135
FokI GGATG 1 cut(s) 331
FspBI CTAG 1 cut(s) 119
HaeIII GGCC 2 cut(s) 148, 404
Hin1II CATG 5 cut(s) 11, 102, 134, 475, 482
HinfI GANTC 2 cut(s) 91, 161
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 2 cut(s) 119, 479
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 41, 371
HpyCH4V TGCA 4 cut(s) 7, 154, 207, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 3 cut(s) 22, 233, 317
Hsp92II CATG 5 cut(s) 11, 102, 134, 475, 482
LpnPI CCDG 4 cut(s) 80, 156, 329, 361
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 2 cut(s) 173, 323
MboII GAAGA 2 cut(s) 38, 383
MluCI AATT 5 cut(s) 122, 126, 256, 269, 338
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 1 cut(s) 126
MroXI GAANNNNTTC 2 cut(s) 126, 381
MseI TTAA 2 cut(s) 125, 357
MslI CAYNNNNRTG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 104
NlaIII CATG 5 cut(s) 11, 102, 134, 475, 482
NmuCI GTSAC 1 cut(s) 323
PagI TCATGA 1 cut(s) 478
PdmI GAANNNNTTC 2 cut(s) 126, 381
PfeI GAWTC 1 cut(s) 91
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
PshBI ATTAAT 1 cut(s) 125
PspPI GGNCC 1 cut(s) 146
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 125, 357
Sau96I GGNCC 1 cut(s) 146
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 1 cut(s) 170
SetI ASST 5 cut(s) 52, 60, 291, 382, 496
SfaNI GCATC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 212
Sse9I AATT 5 cut(s) 122, 126, 256, 269, 338
SspMI CTAG 1 cut(s) 119
TasI AATT 5 cut(s) 122, 126, 256, 269, 338
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 2 cut(s) 125, 357
Tru9I TTAA 2 cut(s) 125, 357
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 1 cut(s) 323
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 4 cut(s) 51, 119, 390, 467
TspRI CASTG 1 cut(s) 258
VspI ATTAAT 1 cut(s) 125
XapI RAATTY 2 cut(s) 269, 338
XbaI TCTAGA 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 2 cut(s) 126, 381
XspI CTAG 1 cut(s) 119
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.