Rh3BG173000
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002557
Physical Location & Seq
Reverse (-)
14850722 .. 14851567
846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG173000.1

Sequence Viewer

Length: 846 bp
ATGATGTCTGATACAAAGTACGAAGGCTCTGCTATGGTTGCTCAATACCAACCACCGCATCTCCGTAGCTCCCAGCCACCTCCATCATCATCATCATCTTGGAAAGCATCCTCTCCAGGATCCATGAAATGCACTCATTGCAAATCAACCTGGCATCTGGTAGAGACTTGTTTCCACTTACATGGCTTCCCGGAATGGTGGGGGGAGCACATTTCCAGAAAGGAAGCACAAGCAAAAGAAGTACAAGGGAAAGCAAGATCATTTAAGCCATCTGGCAACAACAACCCAAAGAGTTATCGATCCAAAGGCAAACCTCAAGTCTCCCTCCAAATTGCTTCCCCAGAACTGAGTTCGAGTACAGCTTCTGATGAACCTCCTCACAGTGTTCACTCTGGTAACTCCACTTTTGCCTTCATGACAACTTCATGTACTCCTTGTTCTTTGCTATCGAGTAACTCTGCCTTTAATTGGAAGGATATGTGGATAATTGATTCAGGTGCAACAGACCATATGACTAATAACCCTAGCAATATTCACCATTTAACCCCAGTTCGCCGGTCTGGCGTAACTAATGCCAATGGCGATTCTTATCCCATTACTGGAGCTGGATCTGTTACTCTTACAAAATCCATTACTCTTGATACAGTGTTACTTGTTCCTTCCCTATCTCATAATCTCTTGTCTGTCAAACAGATAACAAAACAATTTTATTGTTGTGTAATTCTTTACCCGTGGTGTTGTATATTTCAGGACATCCTAACAGGGGAAATCATTGGGCGGGGTATTGAGAAGGGGGGGGTTAAACTACATGGAGATGACCACTGCTGGCAATCGTCGCATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 rve

Protein Analysis

281

Amino Acids

30.82

Weight (kDa)

8.64

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 161 - 235 1.2e-17 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 56, 778
AclWI GGATC 4 cut(s) 114, 127, 294, 616
AfaI GTAC 4 cut(s) 20, 243, 358, 430
AfiI CCNNNNNNNGG 3 cut(s) 468, 599, 763
AjnI CCWGG 2 cut(s) 115, 149
AluBI AGCT 3 cut(s) 69, 362, 605
AluI AGCT 3 cut(s) 69, 362, 605
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 158, 325
AlwI GGATC 4 cut(s) 114, 127, 294, 616
AlwNI CAGNNNCTG 1 cut(s) 365
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 527
BamHI GGATCC 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 2 cut(s) 91, 277
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BciT130I CCWGG 2 cut(s) 117, 151
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 2 cut(s) 158, 325
BfaI CTAG 1 cut(s) 525
BglI GCCNNNNNGGC 1 cut(s) 561
Bme1390I CCNGG 3 cut(s) 117, 151, 191
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 3 cut(s) 117, 151, 191
BmrI ACTGGG 1 cut(s) 542
BmsI GCATC 3 cut(s) 67, 116, 163
BmuI ACTGGG 1 cut(s) 542
BpmI CTGGAG 2 cut(s) 99, 621
BpuEI CTTGAG 1 cut(s) 300
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 3 cut(s) 468, 599, 763
Bse118I RCCGGY 1 cut(s) 555
Bse1I ACTGG 2 cut(s) 548, 604
Bse3DI GCAATG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 2 cut(s) 117, 151
BseCI ATCGAT 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 2 cut(s) 107, 753
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 468, 599, 763
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 2 cut(s) 548, 604
BseRI GAGGAG 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 2 cut(s) 191, 556
BslI CCNNNNNNNGG 3 cut(s) 468, 599, 763
BsmAI GTCTC 2 cut(s) 158, 325
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 4 cut(s) 119, 257, 299, 608
BspACI CCGC 2 cut(s) 56, 778
BspCNI CTCAG 1 cut(s) 339
BspDI ATCGAT 1 cut(s) 298
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 121
BspPI GGATC 4 cut(s) 114, 127, 294, 616
BsrDI GCAATG 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 555
BsrI ACTGG 2 cut(s) 548, 604
BssAI RCCGGY 1 cut(s) 555
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 4 cut(s) 119, 257, 299, 608
Bst2UI CCWGG 2 cut(s) 117, 151
Bst4CI ACNGT 2 cut(s) 383, 646
BstAPI GCANNNNNTGC 1 cut(s) 138
BstC8I GCNNGC 1 cut(s) 827
BstDEI CTNAG 1 cut(s) 347
BstDSI CCRYGG 1 cut(s) 731
BstF5I GGATG 2 cut(s) 107, 753
BstKTI GATC 4 cut(s) 122, 260, 302, 611
BstMAI GTCTC 2 cut(s) 158, 325
BstMBI GATC 4 cut(s) 119, 257, 299, 608
BstMWI GCNNNNNNNGC 4 cut(s) 38, 138, 561, 835
BstNI CCWGG 2 cut(s) 117, 151
BstSCI CCNGG 3 cut(s) 115, 149, 189
BstX2I RGATCY 2 cut(s) 119, 608
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 2 cut(s) 119, 608
Bsu15I ATCGAT 1 cut(s) 298
BsuTUI ATCGAT 1 cut(s) 298
BtgI CCRYGG 1 cut(s) 731
BtsCI GGATG 2 cut(s) 107, 753
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 3 cut(s) 388, 651, 820
Cac8I GCNNGC 1 cut(s) 827
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 1 cut(s) 414
Cfr10I RCCGGY 1 cut(s) 555
ClaI ATCGAT 1 cut(s) 298
Csp6I GTAC 4 cut(s) 19, 242, 357, 429
CviAII CATG 5 cut(s) 124, 182, 415, 426, 809
CviJI RGCY 7 cut(s) 27, 69, 76, 186, 268, 362, 605
CviKI_1 RGCY 7 cut(s) 27, 69, 76, 186, 268, 362, 605
CviQI GTAC 4 cut(s) 19, 242, 357, 429
DdeI CTNAG 1 cut(s) 347
DpnI GATC 4 cut(s) 121, 259, 301, 610
DpnII GATC 4 cut(s) 119, 257, 299, 608
EcoRII CCWGG 2 cut(s) 115, 149
FaeI CATG 5 cut(s) 127, 185, 418, 429, 812
FatI CATG 5 cut(s) 123, 181, 414, 425, 808
FauI CCCGC 1 cut(s) 771
FauNDI CATATG 1 cut(s) 510
FokI GGATG 2 cut(s) 94, 740
FspBI CTAG 1 cut(s) 525
GsaI CCCAGC 1 cut(s) 76
GsuI CTGGAG 2 cut(s) 99, 621
HapII CCGG 2 cut(s) 191, 556
Hin1II CATG 5 cut(s) 127, 185, 418, 429, 812
HinfI GANTC 2 cut(s) 491, 584
HpaII CCGG 2 cut(s) 191, 556
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 2 cut(s) 10, 367
Hpy188III TCNNGA 4 cut(s) 216, 415, 638, 749
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 1 cut(s) 838
HpyAV CCTTC 5 cut(s) 17, 421, 466, 669, 784
HpyCH4III ACNGT 2 cut(s) 383, 646
HpyCH4V TGCA 3 cut(s) 132, 141, 500
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 138, 561, 835
HpyF3I CTNAG 1 cut(s) 347
Hsp92II CATG 5 cut(s) 127, 185, 418, 429, 812
Kzo9I GATC 4 cut(s) 119, 257, 299, 608
LmnI GCTCC 3 cut(s) 74, 205, 602
LweI GCATC 3 cut(s) 67, 116, 163
MaeI CTAG 1 cut(s) 525
MaeIII GTNAC 5 cut(s) 395, 452, 565, 613, 648
MalI GATC 4 cut(s) 121, 259, 301, 610
MboI GATC 4 cut(s) 119, 257, 299, 608
MflI RGATCY 2 cut(s) 119, 608
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 5 cut(s) 330, 466, 486, 704, 720
MnlI CCTC 6 cut(s) 90, 121, 324, 335, 384, 387
MseI TTAA 4 cut(s) 264, 465, 542, 801
MslI CAYNNNNRTG 2 cut(s) 180, 813
MspI CCGG 2 cut(s) 191, 556
MspR9I CCNGG 3 cut(s) 117, 151, 191
MvaI CCWGG 2 cut(s) 117, 151
MwoI GCNNNNNNNGC 4 cut(s) 38, 138, 561, 835
NciI CCSGG 1 cut(s) 191
NdeI CATATG 1 cut(s) 510
NdeII GATC 4 cut(s) 119, 257, 299, 608
NlaIII CATG 5 cut(s) 127, 185, 418, 429, 812
NlaIV GGNNCC 1 cut(s) 121
PagI TCATGA 1 cut(s) 414
PfeI GAWTC 2 cut(s) 491, 584
PfoI TCCNGGA 2 cut(s) 115, 189
Psp6I CCWGG 2 cut(s) 115, 149
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 2 cut(s) 115, 149
PspN4I GGNNCC 1 cut(s) 121
PsrI GAACNNNNNNTAC 2 cut(s) 421, 453
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 2 cut(s) 119, 608
RsaI GTAC 4 cut(s) 20, 243, 358, 430
RsaNI GTAC 4 cut(s) 19, 242, 357, 429
RseI CAYNNNNRTG 2 cut(s) 180, 813
SaqAI TTAA 4 cut(s) 264, 465, 542, 801
Sau3AI GATC 4 cut(s) 119, 257, 299, 608
ScrFI CCNGG 3 cut(s) 117, 151, 191
SduI GDGCHC 1 cut(s) 210
SetI ASST 8 cut(s) 71, 82, 152, 316, 364, 376, 499, 607
SfaNI GCATC 3 cut(s) 67, 116, 163
SmiMI CAYNNNNRTG 2 cut(s) 180, 813
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 5 cut(s) 330, 466, 486, 704, 720
SsiI CCGC 2 cut(s) 56, 778
SspI AATATT 1 cut(s) 532
SspMI CTAG 1 cut(s) 525
StyD4I CCNGG 3 cut(s) 115, 149, 189
TaaI ACNGT 2 cut(s) 383, 646
TaqI TCGA 3 cut(s) 298, 353, 449
TasI AATT 5 cut(s) 330, 466, 486, 704, 720
TatI WGTACW 3 cut(s) 241, 356, 428
TfiI GAWTC 2 cut(s) 491, 584
Tru1I TTAA 4 cut(s) 264, 465, 542, 801
Tru9I TTAA 4 cut(s) 264, 465, 542, 801
TscAI CASTG 3 cut(s) 388, 651, 827
TspDTI ATGAA 4 cut(s) 140, 384, 403, 414
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 3 cut(s) 388, 651, 827
XspI CTAG 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.