pycom14g07330

Perakine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002557
Physical Location & Seq
Reverse (-)
7389559 .. 7390377
819 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07330.3

Sequence Viewer

Length: 429 bp
ATGTTGGCCAAGGCCTCATCTGTAGTAATTGTTAATGGGTGCATTTTGGATTGTTCCAGTGAGTTTTCCATCACTGTGGTACTTGCAGATGGTAATTGTAATACTTGGGCCAAAGTCCAATTCGGTCGAAAAACACCTGAAATTGGTCTCGATCCATCAAAAACCCTAAAGTTGGTGTTCTTTAATTTGTCGAATCTACAGCTTCATCGTCCCCAAACTTGTTTGGCCTTTGTTCCTCAGTTCAAGAGATGTCTAATGGTAGTGATGGTCAATGGAATACCCTTTAGAAACGGCCTATCGTCGTCGTGCACCCACGAAGCCCATCGGTGCAAACTTCACGAAACTGAGGTCAAATCATGTCGGTTTTTGGGTGGAAATGGAAGAGGGAAGGTTGATGAGTATGGTGGTGGTGACAAGTGTTGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

143

Amino Acids

15.55

Weight (kDa)

8.87

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 6
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 143, 172
AgsI TTSAA 1 cut(s) 244
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 307
AlwI GGATC 1 cut(s) 146
AoxI GGCC 5 cut(s) 6, 12, 108, 225, 292
ApaLI GTGCAC 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
AspS9I GGNCC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 311
BccI CCATC 5 cut(s) 77, 83, 163, 259, 330
BceAI ACGGC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 152
BfmI CTRYAG 2 cut(s) 21, 197
BmgT120I GGNCC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 172
Bse1I ACTGG 2 cut(s) 57, 424
BseDI CCNNGG 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 143, 172
BseMII CTCAG 2 cut(s) 251, 336
BseNI ACTGG 2 cut(s) 57, 424
BseSI GKGCMC 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 5 cut(s) 8, 14, 110, 227, 294
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 311
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 143, 172
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 5 cut(s) 8, 14, 110, 227, 294
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 311
Bsp143I GATC 1 cut(s) 151
BspANI GGCC 5 cut(s) 8, 14, 110, 227, 294
BspCNI CTCAG 2 cut(s) 250, 337
BspPI GGATC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 152
BsrI ACTGG 2 cut(s) 57, 424
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 237, 345
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 151
BstMCI CGRYCG 1 cut(s) 127
BstSFI CTRYAG 2 cut(s) 21, 197
BstSLI GKGCMC 1 cut(s) 311
BstXI CCANNNNNNTGG 1 cut(s) 76
BsuRI GGCC 5 cut(s) 8, 14, 110, 227, 294
BtsIMutI CAGTG 2 cut(s) 64, 72
Cfr13I GGNCC 1 cut(s) 108
Csp6I GTAC 1 cut(s) 80
CviAII CATG 1 cut(s) 357
CviJI RGCY 7 cut(s) 8, 14, 110, 202, 227, 294, 320
CviKI_1 RGCY 7 cut(s) 8, 14, 110, 202, 227, 294, 320
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 2 cut(s) 237, 345
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 9
Eco147I AGGCCT 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 1 cut(s) 360
FaiI YATR 2 cut(s) 358, 402
FaqI GGGAC 1 cut(s) 195
FatI CATG 1 cut(s) 356
HaeIII GGCC 5 cut(s) 8, 14, 110, 227, 294
Hin1II CATG 1 cut(s) 360
HinfI GANTC 1 cut(s) 193
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 1 cut(s) 309
Hpy188III TCNNGA 3 cut(s) 149, 244, 338
Hpy8I GTNNAC 1 cut(s) 309
Hpy99I CGWCG 2 cut(s) 304, 307
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 4 cut(s) 42, 86, 309, 330
HpyF3I CTNAG 2 cut(s) 237, 345
Hsp92II CATG 1 cut(s) 360
Kzo9I GATC 1 cut(s) 151
LpnPI CCDG 2 cut(s) 70, 150
MaeIII GTNAC 1 cut(s) 410
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 1 cut(s) 393
MhlI GDGCHC 1 cut(s) 311
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 5 cut(s) 27, 94, 119, 141, 184
MluNI TGGCCA 1 cut(s) 8
MnlI CCTC 4 cut(s) 25, 246, 340, 377
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 2 cut(s) 33, 183
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 8
NdeII GATC 1 cut(s) 151
NlaIII CATG 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 410
PceI AGGCCT 1 cut(s) 14
PfeI GAWTC 1 cut(s) 193
PspPI GGNCC 1 cut(s) 108
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 2 cut(s) 33, 183
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 108
SduI GDGCHC 1 cut(s) 311
SetI ASST 4 cut(s) 139, 204, 351, 393
SfcI CTRYAG 2 cut(s) 21, 197
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 5 cut(s) 27, 94, 119, 141, 184
SseBI AGGCCT 1 cut(s) 14
StuI AGGCCT 1 cut(s) 14
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 3 cut(s) 127, 150, 191
TaqII GACCGA 1 cut(s) 113
TasI AATT 5 cut(s) 27, 94, 119, 141, 184
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 2 cut(s) 33, 183
Tru9I TTAA 2 cut(s) 33, 183
TscAI CASTG 2 cut(s) 64, 79
TseFI GTSAC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 194
TspRI CASTG 2 cut(s) 64, 79
VneI GTGCAC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.