MD05G1344500.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001481
Physical Location & Seq
Reverse (-)
46428388 .. 46433408
5021 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1344500.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGGAGTACTCAAGTGATAGTGATCTCTCTGAGCCCATGTTAGTTCCCAAAACATCTTCATTACAGCAGATAGTAAGCTCGGAACTGGAAGACACACTAAACAACACTGACCTCTCAAACTTTCAGCGTCTCCGAACGGCCACGTGGCTCGAGACAAAGACCCTGTATCGCCTGGCAGCTCCGGCGGTGGTGGTTTACTTGCTCAACAATGTTATATCCATGTCCACCCAAATCTTATGTGGTCATCTTGGCAATCTTGAACTTGCTGCCTCATCTCTTGGCAACACTGGGATTCAAGTTTTCGCCTACGGCCTTATGCTTGGTATGGGTAGTGCAGTGGAAACTCTATGTGGTCAAGCCTATGGAGCACATAAATATGAAATGCTGGGCATATACATGCAGAGATCAACAATCCTCCTCGTGGCAACTGGGATCCCACTTACGATAGTTTACATCTTCTCCAAGCCCCTCTTGCTAGCCCTAGGTGAATCAGCCTCCATCTCAGCCGCAGCCGCGGTTTTCGTATACGGTCTGATCCCTCAAATATTTGCCTACGCTTGCAATTTCCCCATACAAAAATTCCTCCAAGCCCAGAGCATAGTGTTCCCTAGTGCCTACATCTCAGCAGGTGCACTTGTGGTGCACATAGTGTTAAGCTGGGTGGTGGTGTACAAACTGGATTGGGGTTTGCTGGGTGCGGCGTTGATGCTGAGTTTTTCATGGTGGATTATAGTGGTGGCACAGTTTGTGTACATCGTTTGGACTCCAAGGTGCAAGCGTACATGGAGAGGTTTTAGCATTAGGGCATTTTCCGGTCTTTGGAGATTTTTGAAGTTGTCTGCAGCATCGGCCGTAATGTTATGCCTTGAGACTTGGTATTATCAAATTATAGTCTTGATTGCTGGGTTGCTTGAAGATGCTGAAATTGCCTTGGACGCCCTCTCTATCTGCATGACCATAACTGGATGGGTGTTCATGGTTTCTGTGGGGTTCAATGCTGCTGCAAGTGTGAGGGTCGGGAATGAGCTTGGGGCTGGGCATCCAAAAGCGGCTGCATTTGCAGTTGTGATGGTAACTTCAAGCTCCTTCCTCATCTCGGTGGTCTGTGCCATAATTGTACTTGCATTGCGAGATGTCATAAGTTACATTTTCACTGAGGGGGAAACAGTCTCTAATGCAGTCTCAGAACTTTCTCCTTATCTTGCAATATCTATCATTCTTAATGGTATTCAACCTGTTTTGTCAGGGGTGGCTGTTGGGTGTGGATGGCAAGCATTTGTAGCATATGTTAACGTGGGATGCTACTACATCGTTGGCATTCCATTGGGTTGCCTTCTTGGCTTCAAGTTCGACTTTGGAGCTCAGGGAATATGGGGTGGGATGATAGGAGGTACTTTTATACAGACTGTTATCTTAATTTGGGTCACAGTTCGGACTGATTGGAACAAAGAGGTTGAAAAGGTAAGGAATCGTTTGGACAATTGGGAAGACACAAAGGAGCGTTCTCATGAGGATCTTCCGATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

510

Amino Acids

55.55

Weight (kDa)

5.49

Isoelectric Point (pI)

23.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 61 - 221 3.4e-34 MatE
MatE PF01554 283 - 443 3e-32 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 617
AccI GTMKAC 1 cut(s) 525
AccII CGCG 1 cut(s) 515
AciI CCGC 6 cut(s) 185, 507, 513, 515, 698, 1049
AclWI GGATC 4 cut(s) 427, 440, 529, 1521
AcoI YGGCCR 2 cut(s) 138, 849
AcsI RAATTY 1 cut(s) 578
AcvI CACGTG 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 936
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 6 cut(s) 8, 671, 752, 781, 1119, 1393
AfiI CCNNNNNNNGG 3 cut(s) 421, 819, 1096
AgsI TTSAA 9 cut(s) 260, 296, 832, 914, 994, 1080, 1232, 1345, 1457
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 6 cut(s) 78, 179, 657, 1027, 1083, 1361
AluI AGCT 6 cut(s) 78, 179, 657, 1027, 1083, 1361
Alw21I GWGCWC 4 cut(s) 370, 634, 645, 1363
Alw26I GTCTC 5 cut(s) 134, 146, 863, 1174, 1186
Alw44I GTGCAC 2 cut(s) 630, 641
AlwI GGATC 4 cut(s) 427, 440, 529, 1521
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 3 cut(s) 138, 310, 849
ApaLI GTGCAC 2 cut(s) 630, 641
ApeKI GCWGC 7 cut(s) 176, 266, 509, 842, 998, 1001, 1052
ApoI RAATTY 1 cut(s) 578
AseI ATTAAT 1 cut(s) 1524
AspA2I CCTAGG 1 cut(s) 481
AsuHPI GGTGA 1 cut(s) 497
AsuNHI GCTAGC 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 149
AvrII CCTAGG 1 cut(s) 481
BaeGI GKGCMC 2 cut(s) 634, 645
BamHI GGATCC 1 cut(s) 432
BanII GRGCYC 2 cut(s) 36, 1363
BauI CACGAG 1 cut(s) 419
BbrPI CACGTG 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 96, 1494
Bbv12I GWGCWC 4 cut(s) 370, 634, 645, 1363
BbvI GCAGC 7 cut(s) 188, 253, 521, 854, 985, 988, 1039
BccI CCATC 4 cut(s) 506, 960, 1063, 1260
BceAI ACGGC 3 cut(s) 153, 325, 836
BciT130I CCWGG 1 cut(s) 173
BcoDI GTCTC 5 cut(s) 134, 146, 863, 1174, 1186
BfaI CTAG 3 cut(s) 476, 482, 609
BfmI CTRYAG 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 1338
BlnI CCTAGG 1 cut(s) 481
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 1 cut(s) 173
BmrI ACTGGG 2 cut(s) 297, 438
BmsI GCATC 5 cut(s) 696, 854, 907, 1048, 1289
BmtI GCTAGC 1 cut(s) 479
BmuI ACTGGG 2 cut(s) 297, 438
BpiI GAAGAC 2 cut(s) 96, 1494
Bpu10I CCTNAGC 1 cut(s) 1362
BpuEI CTTGAG 1 cut(s) 887
BsaAI YACGTR 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 936
BsaJI CCNNGG 4 cut(s) 481, 513, 767, 930
BsaWI WCCGGW 1 cut(s) 812
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 819, 1096
Bse1I ACTGG 5 cut(s) 90, 292, 433, 681, 967
Bse3DI GCAATG 1 cut(s) 1124
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 4 cut(s) 481, 513, 767, 930
BseGI GGATG 5 cut(s) 971, 1039, 1271, 1304, 1386
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 3 cut(s) 421, 819, 1096
BseMI GCAATG 1 cut(s) 1124
BseMII CTCAG 7 cut(s) 21, 516, 636, 701, 1146, 1197, 1376
BseNI ACTGG 5 cut(s) 90, 292, 433, 681, 967
BseRI GAGGAG 1 cut(s) 407
BseSI GKGCMC 2 cut(s) 634, 645
BseX3I CGGCCG 1 cut(s) 849
BseXI GCAGC 7 cut(s) 188, 253, 521, 854, 985, 988, 1039
BseYI CCCAGC 5 cut(s) 385, 657, 691, 902, 1034
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 515
Bsh1285I CGRYCG 1 cut(s) 852
BshFI GGCC 3 cut(s) 140, 312, 851
BsiEI CGRYCG 1 cut(s) 852
BsiHKAI GWGCWC 4 cut(s) 370, 634, 645, 1363
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 2 cut(s) 182, 813
BslI CCNNNNNNNGG 3 cut(s) 421, 819, 1096
BsmAI GTCTC 5 cut(s) 134, 146, 863, 1174, 1186
BsmBI CGTCTC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 1317
BsnI GGCC 3 cut(s) 140, 312, 851
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 5 cut(s) 36, 370, 634, 645, 1363
Bsp1407I TGTACA 2 cut(s) 669, 750
Bsp143I GATC 5 cut(s) 22, 404, 432, 534, 1513
BspACI CCGC 6 cut(s) 185, 507, 513, 515, 698, 1049
BspANI GGCC 3 cut(s) 140, 312, 851
BspCNI CTCAG 7 cut(s) 22, 515, 635, 702, 1147, 1196, 1375
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 1 cut(s) 1507
BspLI GGNNCC 1 cut(s) 434
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 617
BspOI GCTAGC 1 cut(s) 479
BspPI GGATC 4 cut(s) 427, 440, 529, 1521
BsrDI GCAATG 1 cut(s) 1124
BsrGI TGTACA 2 cut(s) 669, 750
BsrI ACTGG 5 cut(s) 90, 292, 433, 681, 967
BssECI CCNNGG 4 cut(s) 481, 513, 767, 930
BssMI GATC 5 cut(s) 22, 404, 432, 534, 1513
BssNAI GTATAC 1 cut(s) 526
BssNI GRCGYC 1 cut(s) 936
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 3 cut(s) 481, 767, 930
Bst1107I GTATAC 1 cut(s) 526
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 5 cut(s) 530, 744, 1168, 1408, 1429
BstACI GRCGYC 1 cut(s) 936
BstAUI TGTACA 2 cut(s) 669, 750
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 4 cut(s) 477, 559, 776, 1272
BstDEI CTNAG 7 cut(s) 30, 502, 622, 710, 1155, 1183, 1362
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 5 cut(s) 971, 1039, 1271, 1304, 1386
BstFNI CGCG 1 cut(s) 515
BstKTI GATC 5 cut(s) 25, 407, 435, 537, 1516
BstMAI GTCTC 5 cut(s) 134, 146, 863, 1174, 1186
BstMBI GATC 5 cut(s) 22, 404, 432, 534, 1513
BstMCI CGRYCG 1 cut(s) 852
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 2 cut(s) 634, 645
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 7 cut(s) 188, 253, 521, 854, 985, 988, 1039
BstV2I GAAGAC 2 cut(s) 96, 1494
BstX2I RGATCY 2 cut(s) 432, 1513
BstYI RGATCY 2 cut(s) 432, 1513
BstZ17I GTATAC 1 cut(s) 526
BstZI CGGCCG 1 cut(s) 849
BsuRI GGCC 3 cut(s) 140, 312, 851
BtgI CCRYGG 1 cut(s) 513
BtsCI GGATG 5 cut(s) 971, 1039, 1271, 1304, 1386
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 4 cut(s) 105, 285, 342, 1152
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 4 cut(s) 477, 559, 776, 1272
CciI TCATGA 1 cut(s) 1507
Cfr42I CCGCGG 1 cut(s) 516
CseI GACGC 2 cut(s) 116, 944
Csp6I GTAC 6 cut(s) 7, 670, 751, 780, 1118, 1392
CviAII CATG 8 cut(s) 37, 220, 397, 720, 783, 952, 976, 1508
CviQI GTAC 6 cut(s) 7, 670, 751, 780, 1118, 1392
DdeI CTNAG 7 cut(s) 30, 502, 622, 710, 1155, 1183, 1362
DpnI GATC 5 cut(s) 24, 406, 434, 536, 1515
DpnII GATC 5 cut(s) 22, 404, 432, 534, 1513
DraIII CACNNNGTG 1 cut(s) 649
EaeI YGGCCR 2 cut(s) 138, 849
EagI CGGCCG 1 cut(s) 849
Ecl136II GAGCTC 1 cut(s) 1361
EclXI CGGCCG 1 cut(s) 849
Eco130I CCWWGG 3 cut(s) 481, 767, 930
Eco24I GRGCYC 2 cut(s) 36, 1363
Eco52I CGGCCG 1 cut(s) 849
Eco53kI GAGCTC 1 cut(s) 1361
Eco72I CACGTG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 1361
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 3 cut(s) 481, 767, 930
EcoT38I GRGCYC 2 cut(s) 36, 1363
ErhI CCWWGG 3 cut(s) 481, 767, 930
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 8 cut(s) 40, 223, 400, 723, 786, 955, 979, 1511
FalI AAGNNNNNCTT 4 cut(s) 455, 487, 1337, 1369
FatI CATG 8 cut(s) 36, 219, 396, 719, 782, 951, 975, 1507
FauNDI CATATG 1 cut(s) 1285
FblI GTMKAC 1 cut(s) 525
FokI GGATG 5 cut(s) 978, 1026, 1278, 1311, 1393
FriOI GRGCYC 2 cut(s) 36, 1363
FspBI CTAG 3 cut(s) 476, 482, 609
GsaI CCCAGC 5 cut(s) 389, 661, 695, 906, 1038
HaeIII GGCC 3 cut(s) 140, 312, 851
HapII CCGG 2 cut(s) 182, 813
HgaI GACGC 2 cut(s) 116, 944
Hin1I GRCGYC 1 cut(s) 936
Hin1II CATG 8 cut(s) 40, 223, 400, 723, 786, 955, 979, 1511
HincII GTYRAC 1 cut(s) 1291
HindII GTYRAC 1 cut(s) 1291
HinfI GANTC 4 cut(s) 292, 488, 763, 1468
HpaI GTTAAC 1 cut(s) 1291
HpaII CCGG 2 cut(s) 182, 813
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 9 cut(s) 196, 225, 451, 526, 632, 643, 670, 751, 1291
Hpy188I TCNGA 7 cut(s) 31, 82, 134, 534, 1186, 1434, 1521
Hpy188III TCNNGA 5 cut(s) 151, 257, 895, 1018, 1508
Hpy8I GTNNAC 9 cut(s) 196, 225, 451, 526, 632, 643, 670, 751, 1291
HpyAV CCTTC 2 cut(s) 1096, 1343
HpyCH4III ACNGT 5 cut(s) 530, 744, 1168, 1408, 1429
HpyCH4IV ACGT 2 cut(s) 143, 1293
HpyF3I CTNAG 7 cut(s) 30, 502, 622, 710, 1155, 1183, 1362
HpySE526I ACGT 2 cut(s) 143, 1293
Hsp92I GRCGYC 1 cut(s) 936
Hsp92II CATG 8 cut(s) 40, 223, 400, 723, 786, 955, 979, 1511
KspAI GTTAAC 1 cut(s) 1291
KspI CCGCGG 1 cut(s) 516
Kzo9I GATC 5 cut(s) 22, 404, 432, 534, 1513
LmnI GCTCC 5 cut(s) 184, 365, 1088, 1358, 1498
Lsp1109I GCAGC 7 cut(s) 188, 253, 521, 854, 985, 988, 1039
LweI GCATC 5 cut(s) 696, 854, 907, 1048, 1289
MaeI CTAG 3 cut(s) 476, 482, 609
MaeII ACGT 2 cut(s) 143, 1293
MaeIII GTNAC 3 cut(s) 1072, 1142, 1423
MalI GATC 5 cut(s) 24, 406, 434, 536, 1515
MboI GATC 5 cut(s) 22, 404, 432, 534, 1513
MboII GAAGA 6 cut(s) 48, 101, 448, 926, 1499, 1508
MfeI CAATTG 1 cut(s) 1480
MflI RGATCY 2 cut(s) 432, 1513
MhlI GDGCHC 5 cut(s) 36, 370, 634, 645, 1363
MluCI AATT 8 cut(s) 562, 578, 885, 924, 1113, 1416, 1480, 1525
MlyI GAGTC 1 cut(s) 757
MseI TTAA 5 cut(s) 653, 1221, 1290, 1415, 1524
MslI CAYNNNNRTG 3 cut(s) 375, 395, 1097
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 2 cut(s) 182, 813
MspR9I CCNGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 1480
Mva1269I GAATGC 1 cut(s) 1317
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 515
NdeI CATATG 1 cut(s) 1285
NdeII GATC 5 cut(s) 22, 404, 432, 534, 1513
NheI GCTAGC 1 cut(s) 475
NlaIII CATG 8 cut(s) 40, 223, 400, 723, 786, 955, 979, 1511
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 1423
NspI RCATGY 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 1507
PaqCI CACCTGC 1 cut(s) 617
PctI GAATGC 1 cut(s) 1317
PfeI GAWTC 3 cut(s) 292, 488, 1468
PleI GAGTC 1 cut(s) 757
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
PpsI GAGTC 1 cut(s) 757
Ppu21I YACGTR 1 cut(s) 144
PshBI ATTAAT 1 cut(s) 1524
Psp124BI GAGCTC 1 cut(s) 1363
Psp6I CCWGG 1 cut(s) 171
PspCI CACGTG 1 cut(s) 144
PspFI CCCAGC 5 cut(s) 385, 657, 691, 902, 1034
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 434
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 2 cut(s) 432, 1513
RsaI GTAC 6 cut(s) 8, 671, 752, 781, 1119, 1393
RsaNI GTAC 6 cut(s) 7, 670, 751, 780, 1118, 1392
RseI CAYNNNNRTG 3 cut(s) 375, 395, 1097
SacI GAGCTC 1 cut(s) 1363
SacII CCGCGG 1 cut(s) 516
SaqAI TTAA 5 cut(s) 653, 1221, 1290, 1415, 1524
Sau3AI GATC 5 cut(s) 22, 404, 432, 534, 1513
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 5 cut(s) 36, 370, 634, 645, 1363
SfaNI GCATC 5 cut(s) 696, 854, 907, 1048, 1289
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 516
SgrBI CCGCGG 1 cut(s) 516
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 3 cut(s) 375, 395, 1097
SmlI CTYRAG 3 cut(s) 10, 149, 866
SmoI CTYRAG 3 cut(s) 10, 149, 866
Sse9I AATT 8 cut(s) 562, 578, 885, 924, 1113, 1416, 1480, 1525
SsiI CCGC 6 cut(s) 185, 507, 513, 515, 698, 1049
SspI AATATT 1 cut(s) 546
SspMI CTAG 3 cut(s) 476, 482, 609
SstI GAGCTC 1 cut(s) 1363
StyD4I CCNGG 1 cut(s) 171
StyI CCWWGG 3 cut(s) 481, 767, 930
TaaI ACNGT 5 cut(s) 530, 744, 1168, 1408, 1429
TaiI ACGT 2 cut(s) 146, 1296
TaqI TCGA 2 cut(s) 150, 1350
TasI AATT 8 cut(s) 562, 578, 885, 924, 1113, 1416, 1480, 1525
TatI WGTACW 4 cut(s) 6, 669, 750, 1117
TauI GCSGC 4 cut(s) 509, 515, 701, 1052
TfiI GAWTC 3 cut(s) 292, 488, 1468
Tru1I TTAA 5 cut(s) 653, 1221, 1290, 1415, 1524
Tru9I TTAA 5 cut(s) 653, 1221, 1290, 1415, 1524
TscAI CASTG 4 cut(s) 112, 292, 342, 1159
TseFI GTSAC 1 cut(s) 1423
TseI GCWGC 7 cut(s) 176, 266, 509, 842, 998, 1001, 1052
Tsp45I GTSAC 1 cut(s) 1423
TspDTI ATGAA 4 cut(s) 48, 393, 708, 964
TspRI CASTG 4 cut(s) 112, 292, 342, 1159
VneI GTGCAC 2 cut(s) 630, 641
VspI ATTAAT 1 cut(s) 1524
XapI RAATTY 1 cut(s) 578
XceI RCATGY 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 149
XmaJI CCTAGG 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 525
XspI CTAG 3 cut(s) 476, 482, 609
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.