Rmu_sc0006842.1_g000002

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006842.1
OG0001481
Physical Location & Seq
Forward (+)
7647 .. 11343
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006842.1_g000002.1.cds

Sequence Viewer

Length: 1194 bp
atgggaagtgcggtggaaacgctctgtggtcaagcctatggagcaaagaaatatgacatgctaggcgtatatctgcagcgttcaacgatcctgctaatggcaactgggatcccagttatgttcatctacatcttctccgagtccatcttgattgccctaggcgaatcggccaccattgcctctgcagccgcaatcttcgtttacggacttatcccccaaatctttgcctacgcttgcaatttccccatacaaaaattcctccaggctcagagcatcgtgttcccgagcgcgtacatatccgcggctgccctcgtggtgcatgtggtgctgagttgggtagtgatttttaagttgggatggggcttgttaggcgcatcgctgatgttgagcttttcgtggtggattatagtggtggcgcagtttgtttacatattgctgagtccaaggtgtaaacgtacttggacaggttttagcatgcaggctttttctgggctttggacttttctgaagctatctgttgcatctgccgtaatgctttgcctcgagacttggtactatcagattatcgttttgattgctgggttgcttgaagacgctgaaattgccttggacgctctctctgtttgcatgaccatatctggatgggtgttcatggttgctgtgggtttcaatgctgctgcaagtgtaaggattgggaatgagctcggggcgggacatccaaagtcggccgcatttgcagtaataatagtgacatcaacctcgttcatcatctcagtagtgtgtgccatagttgtgcttgcactacgaaatgtcataagctacgcgttcaccgagggcgagactgtgtccaatgcagtctcagccctatctccttaccttgctgtctctattatgctcaacggcatccaacctgttttgtctggggtggctgttgggtgtggatggcaagcatttgtggcatatgttaacgtcggatgttactacattattggtattcctttgggttgtgttcttggcttcaagtttgactttggagcccagggaatatggtctggaatgataggagggacatttatacagacggcgatcttattgtgggtcacacttcggactgattggaataaagaggtcgcaaatgcaaagaatcgtttggacaagtgggatgacaagaaagagcctcttctcacagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

397

Amino Acids

42.93

Weight (kDa)

6.72

Isoelectric Point (pI)

24.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 290, 302, 824
AciI CCGC 6 cut(s) 11, 189, 300, 302, 710, 729
AclWI GGATC 3 cut(s) 82, 103, 116
AcoI YGGCCR 2 cut(s) 168, 726
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 527
AfaI GTAC 3 cut(s) 293, 457, 554
AfiI CCNNNNNNNGG 1 cut(s) 97
AflIII ACRYGT 1 cut(s) 822
AgsI TTSAA 4 cut(s) 84, 590, 670, 1021
AjnI CCWGG 2 cut(s) 261, 1038
AluBI AGCT 4 cut(s) 390, 511, 703, 819
AluI AGCT 4 cut(s) 390, 511, 703, 819
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 4 cut(s) 539, 833, 862, 889
AlwI GGATC 3 cut(s) 82, 103, 116
Ama87I CYCGRG 3 cut(s) 283, 542, 704
AoxI GGCC 2 cut(s) 168, 726
ApeKI GCWGC 5 cut(s) 76, 185, 305, 674, 677
ApoI RAATTY 1 cut(s) 254
AspA2I CCTAGG 1 cut(s) 157
AspLEI GCGC 3 cut(s) 290, 374, 418
AsuHPI GGTGA 1 cut(s) 820
AvaI CYCGRG 3 cut(s) 283, 542, 704
AvrII CCTAGG 1 cut(s) 157
BamHI GGATCC 1 cut(s) 108
BanII GRGCYC 2 cut(s) 705, 1039
BauI CACGAG 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 705
BbvI GCAGC 5 cut(s) 88, 197, 292, 661, 664
BccI CCATC 4 cut(s) 152, 351, 636, 936
BceAI ACGGC 3 cut(s) 512, 916, 1098
BciT130I CCWGG 2 cut(s) 263, 1040
BcoDI GTCTC 4 cut(s) 539, 833, 862, 889
BfaI CTAG 2 cut(s) 62, 158
BfmI CTRYAG 2 cut(s) 74, 183
BisI GCNGC 8 cut(s) 77, 186, 189, 303, 306, 675, 678, 729
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 8 cut(s) 78, 187, 190, 304, 307, 676, 679, 730
Bme1390I CCNGG 2 cut(s) 263, 1040
BmeT110I CYCGRG 3 cut(s) 283, 542, 704
BmiI GGNNCC 2 cut(s) 110, 1036
BmrFI CCNGG 2 cut(s) 263, 1040
BmrI ACTGGG 2 cut(s) 107, 114
BmsI GCATC 4 cut(s) 282, 383, 530, 912
BmuI ACTGGG 2 cut(s) 107, 114
BpiI GAAGAC 1 cut(s) 597
BpmI CTGGAG 1 cut(s) 245
BsaJI CCNNGG 7 cut(s) 157, 300, 443, 606, 831, 1038, 1039
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 2 cut(s) 109, 113
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 2 cut(s) 263, 1040
BseDI CCNNGG 7 cut(s) 157, 300, 443, 606, 831, 1038, 1039
BseGI GGATG 7 cut(s) 362, 647, 715, 903, 947, 980, 1168
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 5 cut(s) 281, 320, 428, 786, 873
BseNI ACTGG 2 cut(s) 109, 113
BseX3I CGGCCG 1 cut(s) 726
BseXI GCAGC 5 cut(s) 88, 197, 292, 661, 664
BseYI CCCAGC 1 cut(s) 578
Bsh1236I CGCG 3 cut(s) 290, 302, 824
Bsh1285I CGRYCG 1 cut(s) 729
BshFI GGCC 2 cut(s) 170, 728
BsiEI CGRYCG 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 705
BsiHKCI CYCGRG 3 cut(s) 283, 542, 704
BslFI GGGAC 2 cut(s) 726, 1081
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 4 cut(s) 539, 833, 862, 889
BsmFI GGGAC 2 cut(s) 726, 1081
BsnI GGCC 2 cut(s) 170, 728
BsoBI CYCGRG 3 cut(s) 283, 542, 704
Bsp1286I GDGCHC 2 cut(s) 705, 1039
Bsp143I GATC 3 cut(s) 87, 108, 1086
BspACI CCGC 6 cut(s) 11, 189, 300, 302, 710, 729
BspANI GGCC 2 cut(s) 170, 728
BspCNI CTCAG 5 cut(s) 280, 321, 429, 785, 872
BspFNI CGCG 3 cut(s) 290, 302, 824
BspLI GGNNCC 2 cut(s) 110, 1036
BspMAI CTGCAG 2 cut(s) 78, 187
BspPI GGATC 3 cut(s) 82, 103, 116
BsrDI GCAATG 1 cut(s) 174
BsrI ACTGG 2 cut(s) 109, 113
BssECI CCNNGG 7 cut(s) 157, 300, 443, 606, 831, 1038, 1039
BssMI GATC 3 cut(s) 87, 108, 1086
BssSI CACGAG 1 cut(s) 311
BssT1I CCWWGG 3 cut(s) 157, 443, 606
Bst2BI CACGAG 1 cut(s) 311
Bst2UI CCWGG 2 cut(s) 263, 1040
Bst4CI ACNGT 1 cut(s) 844
Bst6I CTCTTC 1 cut(s) 1185
BstAPI GCANNNNNTGC 1 cut(s) 325
BstC8I GCNNGC 5 cut(s) 235, 476, 480, 798, 948
BstDEI CTNAG 5 cut(s) 267, 329, 437, 772, 859
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 7 cut(s) 362, 647, 715, 903, 947, 980, 1168
BstFNI CGCG 3 cut(s) 290, 302, 824
BstHHI GCGC 3 cut(s) 290, 374, 418
BstKTI GATC 3 cut(s) 90, 111, 1089
BstMAI GTCTC 4 cut(s) 539, 833, 862, 889
BstMBI GATC 3 cut(s) 87, 108, 1086
BstMCI CGRYCG 1 cut(s) 729
BstNI CCWGG 2 cut(s) 263, 1040
BstNSI RCATGY 3 cut(s) 61, 323, 478
BstSCI CCNGG 2 cut(s) 261, 1038
BstSFI CTRYAG 2 cut(s) 74, 183
BstUI CGCG 3 cut(s) 290, 302, 824
BstV1I GCAGC 5 cut(s) 88, 197, 292, 661, 664
BstV2I GAAGAC 1 cut(s) 597
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BstZI CGGCCG 1 cut(s) 726
BsuRI GGCC 2 cut(s) 170, 728
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 7 cut(s) 362, 647, 715, 903, 947, 980, 1168
Cac8I GCNNGC 5 cut(s) 235, 476, 480, 798, 948
CfoI GCGC 3 cut(s) 290, 374, 418
Cfr42I CCGCGG 1 cut(s) 303
CseI GACGC 2 cut(s) 602, 620
Csp6I GTAC 3 cut(s) 292, 456, 553
CviAII CATG 5 cut(s) 58, 320, 475, 628, 652
CviQI GTAC 3 cut(s) 292, 456, 553
DdeI CTNAG 5 cut(s) 267, 329, 437, 772, 859
DpnI GATC 3 cut(s) 89, 110, 1088
DpnII GATC 3 cut(s) 87, 108, 1086
EaeI YGGCCR 2 cut(s) 168, 726
EagI CGGCCG 1 cut(s) 726
Eam1104I CTCTTC 1 cut(s) 1185
EarI CTCTTC 1 cut(s) 1185
Ecl136II GAGCTC 1 cut(s) 703
EclXI CGGCCG 1 cut(s) 726
Eco130I CCWWGG 3 cut(s) 157, 443, 606
Eco24I GRGCYC 2 cut(s) 705, 1039
Eco52I CGGCCG 1 cut(s) 726
Eco53kI GAGCTC 1 cut(s) 703
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 3 cut(s) 283, 542, 704
EcoICRI GAGCTC 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 261, 1038
EcoT14I CCWWGG 3 cut(s) 157, 443, 606
EcoT38I GRGCYC 2 cut(s) 705, 1039
ErhI CCWWGG 3 cut(s) 157, 443, 606
FaeI CATG 5 cut(s) 61, 323, 478, 631, 655
FalI AAGNNNNNCTT 3 cut(s) 1013, 1045, 1164
FaqI GGGAC 2 cut(s) 726, 1081
FatI CATG 5 cut(s) 57, 319, 474, 627, 651
FauI CCCGC 1 cut(s) 703
FauNDI CATATG 1 cut(s) 961
Fnu4HI GCNGC 8 cut(s) 77, 186, 189, 303, 306, 675, 678, 729
FokI GGATG 7 cut(s) 369, 654, 702, 890, 954, 987, 1175
FriOI GRGCYC 2 cut(s) 705, 1039
Fsp4HI GCNGC 8 cut(s) 77, 186, 189, 303, 306, 675, 678, 729
FspBI CTAG 2 cut(s) 62, 158
GlaI GCGC 3 cut(s) 289, 373, 417
GluI GCNGC 8 cut(s) 77, 186, 189, 303, 306, 675, 678, 729
GsaI CCCAGC 1 cut(s) 582
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 2 cut(s) 170, 728
HgaI GACGC 2 cut(s) 602, 620
HhaI GCGC 3 cut(s) 290, 374, 418
Hin1II CATG 5 cut(s) 61, 323, 478, 631, 655
Hin6I GCGC 3 cut(s) 288, 372, 416
HinP1I GCGC 3 cut(s) 288, 372, 416
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HinfI GANTC 4 cut(s) 140, 164, 439, 1144
HpaI GTTAAC 1 cut(s) 967
HphI GGTGA 1 cut(s) 820
Hpy166II GTNNAC 5 cut(s) 202, 427, 452, 828, 967
Hpy188I TCNGA 6 cut(s) 139, 270, 507, 561, 974, 1110
Hpy188III TCNNGA 5 cut(s) 148, 283, 544, 639, 1053
Hpy8I GTNNAC 5 cut(s) 202, 427, 452, 828, 967
Hpy99I CGWCG 1 cut(s) 974
HpyCH4III ACNGT 1 cut(s) 844
HpyCH4IV ACGT 2 cut(s) 454, 969
HpyF3I CTNAG 5 cut(s) 267, 329, 437, 772, 859
HpySE526I ACGT 2 cut(s) 454, 969
Hsp92II CATG 5 cut(s) 61, 323, 478, 631, 655
HspAI GCGC 3 cut(s) 288, 372, 416
KspAI GTTAAC 1 cut(s) 967
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 3 cut(s) 87, 108, 1086
LmnI GCTCC 2 cut(s) 41, 1034
Lsp1109I GCAGC 5 cut(s) 88, 197, 292, 661, 664
LweI GCATC 4 cut(s) 282, 383, 530, 912
MaeI CTAG 2 cut(s) 62, 158
MaeII ACGT 2 cut(s) 454, 969
MaeIII GTNAC 3 cut(s) 748, 977, 1099
MalI GATC 3 cut(s) 89, 110, 1088
MboI GATC 3 cut(s) 87, 108, 1086
MboII GAAGA 4 cut(s) 124, 187, 602, 1172
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 2 cut(s) 705, 1039
MluCI AATT 3 cut(s) 238, 254, 600
MluI ACGCGT 1 cut(s) 822
MlyI GAGTC 2 cut(s) 149, 448
MmeI TCCRAC 2 cut(s) 931, 952
MnlI CCTC 9 cut(s) 190, 269, 320, 551, 769, 826, 1058, 1120, 1188
MseI TTAA 2 cut(s) 348, 966
MslI CAYNNNNRTG 1 cut(s) 791
MspA1I CMGCKG 1 cut(s) 302
MspR9I CCNGG 2 cut(s) 263, 1040
MvaI CCWGG 2 cut(s) 263, 1040
MvnI CGCG 3 cut(s) 290, 302, 824
NdeI CATATG 1 cut(s) 961
NdeII GATC 3 cut(s) 87, 108, 1086
NlaIII CATG 5 cut(s) 61, 323, 478, 631, 655
NlaIV GGNNCC 2 cut(s) 110, 1036
NmuCI GTSAC 2 cut(s) 748, 1099
NspI RCATGY 3 cut(s) 61, 323, 478
PaeI GCATGC 1 cut(s) 478
PaeR7I CTCGAG 1 cut(s) 542
PasI CCCWGGG 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 828
PfeI GAWTC 2 cut(s) 164, 1144
PflFI GACNNNGTC 1 cut(s) 844
PkrI GCNGC 8 cut(s) 78, 187, 190, 304, 307, 676, 679, 730
PleI GAGTC 2 cut(s) 148, 447
PpsI GAGTC 2 cut(s) 148, 447
Psp124BI GAGCTC 1 cut(s) 705
Psp6I CCWGG 2 cut(s) 261, 1038
PspFI CCCAGC 1 cut(s) 578
PspGI CCWGG 2 cut(s) 261, 1038
PspN4I GGNNCC 2 cut(s) 110, 1036
PstI CTGCAG 2 cut(s) 78, 187
PsuI RGATCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 844
RsaI GTAC 3 cut(s) 293, 457, 554
RsaNI GTAC 3 cut(s) 292, 456, 553
RseI CAYNNNNRTG 1 cut(s) 791
SacI GAGCTC 1 cut(s) 705
SacII CCGCGG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 348, 966
SatI GCNGC 8 cut(s) 77, 186, 189, 303, 306, 675, 678, 729
Sau3AI GATC 3 cut(s) 87, 108, 1086
SchI GAGTC 2 cut(s) 149, 448
ScrFI CCNGG 2 cut(s) 263, 1040
SduI GDGCHC 2 cut(s) 705, 1039
SfaNI GCATC 4 cut(s) 282, 383, 530, 912
SfcI CTRYAG 2 cut(s) 74, 183
Sfr274I CTCGAG 1 cut(s) 542
Sfr303I CCGCGG 1 cut(s) 303
SgrBI CCGCGG 1 cut(s) 303
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SphI GCATGC 1 cut(s) 478
Sse9I AATT 3 cut(s) 238, 254, 600
SsiI CCGC 6 cut(s) 11, 189, 300, 302, 710, 729
SspMI CTAG 2 cut(s) 62, 158
SstI GAGCTC 1 cut(s) 705
StyD4I CCNGG 2 cut(s) 261, 1038
StyI CCWWGG 3 cut(s) 157, 443, 606
TaaI ACNGT 1 cut(s) 844
TaiI ACGT 2 cut(s) 457, 972
TaqI TCGA 1 cut(s) 543
TasI AATT 3 cut(s) 238, 254, 600
TauI GCSGC 3 cut(s) 191, 305, 731
TfiI GAWTC 2 cut(s) 164, 1144
Tru1I TTAA 2 cut(s) 348, 966
Tru9I TTAA 2 cut(s) 348, 966
TseFI GTSAC 2 cut(s) 748, 1099
TseI GCWGC 5 cut(s) 76, 185, 305, 674, 677
Tsp45I GTSAC 2 cut(s) 748, 1099
TspDTI ATGAA 3 cut(s) 112, 640, 754
TspGWI ACGGA 1 cut(s) 219
Tth111I GACNNNGTC 1 cut(s) 844
XapI RAATTY 1 cut(s) 254
XceI RCATGY 3 cut(s) 61, 323, 478
XhoI CTCGAG 1 cut(s) 542
XmaJI CCTAGG 1 cut(s) 157
XspI CTAG 2 cut(s) 62, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.