Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0001481

Physical Location & Seq

Genomic Coordinates Reverse (-)

2300302 .. 2301618

Gene Structure 1317 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g03100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 900 bp

ATGCTCACGAAGAGCATGCAAGCGGCGAGCTGGAACAAGTACTATGCGACACCGGCAAGCCGTTTTCACAGCGGCTTAAACCCGCCCTATGGATCGAGTCCAAGCTCCTCGTCGTCCTCGCCGCCCCTGCCATCATCGTGTACATCGATCAACTATGTCATGTCCATGTCCACCCAAATGTTTTGCGGCCACCTCGGTAATCTCGAGCTCGCCGCCTCCTCCCTCGGCAACAATGGCATCCAAATCTTCGCCTATGGTCTTTTGTTAGGTATGGGAAGCGCAGTGGAAACCCTATGTGGACAAGCATACGGTGCCCAAAAGTACGAAATGTTAGGCATATACCTACAAAGATCAGCAGTCCTCCTATTTTTCCCTGGTGTACTCCTCACCATTATTTACGTCTTCTCCGAACCAATCTTGCTTTTACTTGGTGAATCTCCGAGCATTGCTTCTTCAGCTGCAGTTTATGTGTACGGACTGATCCCTCAGATCTTTGCCTACGTTGTCAACTTCCCGATCCAAAAGTTCATGCAATCTCAAAGCATAGTTGCCCCAAGTGCTTATATATCGACTGGAACCCTAGTTATCCACATTTTGCTCAGCTGGGTTGCTGTGTACAAATTGGGGTTGGGTTTGCTGGGTGCGTCGCTGGTGCTCAGTCTGTCATGGTGGATCACCGTGGTGGCACAGTTCGTGTACATTGTTAAGAGTGACAGGTGTAAGAACACGTGGACACGGTTTTCGTGGCAAGCTTTCAGCGGATTGTACAGTTTTTTCAAGTTGTCTGTGGCGTCTGCTATCATGCTTTGCCTGGAGACGTGGTACTTTCAGATTCTTGTTTTGCTTGCTGGGTTGCTGGAAAACCCTGAGCTGGCCCTTGACTCCCTCTCCATCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0016020 GO:0022857 GO:0031090 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MatE

Protein Analysis

300

Amino Acids

32.63

Weight (kDa)

8.2

Isoelectric Point (pI)

38.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MatE	PF01554	50 - 203	2e-34	MatE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001481)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21690
fragaria_vesca	FvH4_3g02250 FvH4_3g02250 FvH4_6g43220 FvH4_6g43220
malus_domestica	MD05G1344500.v1.1 MD05G1344900.v1.1 MD09G1108500.v1.1 MD17G1096400.v1.1
prunus_persica	Prupe.3G217600_v2.0.a1 Prupe.3G217600_v2.0.a1 Prupe.3G217600_v2.0.a1 Prupe.4G021900_v2.0.a1
pyrus_communis	pycom05g31380 pycom09g03100 pycom17g09250
rosa_chinensis	RchiOBHm_Chr2g0159351 RchiOBHm_Chr2g0159471 RchiOBHm_Chr5g0003321 RchiOBHm_Chr5g0003371
rosa_laevigata	RLG00000021157 RLG00000021161 RLG00000031126 RLG00000031130
rosa_multiflora	Rmu_sc0004800.1_g000007 Rmu_sc0006842.1_g000002 Rmu_sc0007710.1_g000003 Rmu_sc0010917.1_g000005
rosa_roxburghii	Rroxscaffold_1G00072650
rosa_rugosa	Rorug02G0475900 Rorug02G0476200 Rorug02G0476300 Rorug02G0476500 Rorug02G0476600 Rorug02G0476700 Rorug04G0402700.1 Rorug04G0402800
rosa_samantha	Rh2AG542800 Rh2AG543100 Rh2BG555300 Rh2BG555800 Rh2DG564900 Rh2DG565200 Rh5AG028800 Rh5BG028900 Rh5DG029400
rosa_wichuraiana	Rw2G044870 Rw2G044920 Rw5G002370 Rw5G002680 Rw5G002730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	311
AciI	CCGC	7 cut(s)	23, 72, 83, 122, 186, 213, 759
AclWI	GGATC	4 cut(s)	100, 475, 511, 680
AcoI	YGGCCR	1 cut(s)	187
AcuI	CTGAAG	1 cut(s)	438
AcvI	CACGTG	1 cut(s)	729
AcyI	GRCGYC	1 cut(s)	791
AfaI	GTAC	9 cut(s)	41, 142, 323, 381, 473, 617, 698, 767, 824
AfiI	CCNNNNNNNGG	2 cut(s)	89, 871
AflIII	ACRYGT	1 cut(s)	726
AgsI	TTSAA	1 cut(s)	778
AjiI	CACGTC	1 cut(s)	819
AjnI	CCWGG	2 cut(s)	373, 810
AleI	CACNNNNGTG	1 cut(s)	680
AluBI	AGCT	7 cut(s)	30, 105, 208, 458, 603, 752, 871
AluI	AGCT	7 cut(s)	30, 105, 208, 458, 603, 752, 871
Alw21I	GWGCWC	2 cut(s)	210, 657
Alw26I	GTCTC	1 cut(s)	809
AlwI	GGATC	4 cut(s)	100, 475, 511, 680
Ama87I	CYCGRG	1 cut(s)	203
AoxI	GGCC	2 cut(s)	187, 873
ApeKI	GCWGC	1 cut(s)	458
ArsI	GACNNNNNNTTYG	2 cut(s)	724, 756
AspLEI	GCGC	1 cut(s)	281
AspS9I	GGNCC	1 cut(s)	874
AsuHPI	GGTGA	3 cut(s)	379, 443, 667
AvaI	CYCGRG	1 cut(s)	203
BaeGI	GKGCMC	1 cut(s)	316
BanI	GGYRCC	1 cut(s)	311
BanII	GRGCYC	1 cut(s)	210
BbrPI	CACGTG	1 cut(s)	729
BbsI	GAAGAC	1 cut(s)	394
Bbv12I	GWGCWC	2 cut(s)	210, 657
BbvI	GCAGC	1 cut(s)	445
BccI	CCATC	2 cut(s)	139, 899
BceAI	ACGGC	1 cut(s)	45
BcgI	CGANNNNNNTGC	1 cut(s)	32
BciT130I	CCWGG	2 cut(s)	375, 812
BcoDI	GTCTC	1 cut(s)	809
BfaI	CTAG	1 cut(s)	581
BfmI	CTRYAG	1 cut(s)	459
BglII	AGATCT	1 cut(s)	489
BisI	GCNGC	6 cut(s)	24, 73, 122, 187, 213, 459
BlpI	GCTNAGC	1 cut(s)	599
BlsI	GCNGC	6 cut(s)	25, 74, 123, 188, 214, 460
BmcAI	AGTACT	1 cut(s)	41
Bme1390I	CCNGG	2 cut(s)	375, 812
BmeT110I	CYCGRG	1 cut(s)	203
BmgBI	CACGTC	1 cut(s)	819
BmgT120I	GGNCC	1 cut(s)	874
BmiI	GGNNCC	2 cut(s)	313, 577
BmrFI	CCNGG	2 cut(s)	375, 812
BmsI	GCATC	1 cut(s)	246
BpiI	GAAGAC	1 cut(s)	394
BpmI	CTGGAG	1 cut(s)	833
Bpu10I	CCTNAGC	1 cut(s)	867
Bpu1102I	GCTNAGC	1 cut(s)	599
Bsa29I	ATCGAT	1 cut(s)	146
BsaAI	YACGTR	1 cut(s)	729
BsaHI	GRCGYC	1 cut(s)	791
BsaJI	CCNNGG	4 cut(s)	193, 223, 373, 678
BsaXI	ACNNNNNCTCC	5 cut(s)	389, 419, 806, 836, 872
Bsc4I	CCNNNNNNNGG	2 cut(s)	89, 871
Bse118I	RCCGGY	1 cut(s)	52
Bse1I	ACTGG	1 cut(s)	577
Bse3DI	GCAATG	1 cut(s)	444
BseBI	CCWGG	2 cut(s)	375, 812
BseCI	ATCGAT	1 cut(s)	146
BseDI	CCNNGG	4 cut(s)	193, 223, 373, 678
BseGI	GGATG	1 cut(s)	237
BseLI	CCNNNNNNNGG	2 cut(s)	89, 871
BseMI	GCAATG	1 cut(s)	444
BseMII	CTCAG	4 cut(s)	500, 613, 670, 858
BseNI	ACTGG	1 cut(s)	577
BseRI	GAGGAG	3 cut(s)	97, 208, 374
BseSI	GKGCMC	1 cut(s)	316
BseXI	GCAGC	1 cut(s)	445
BseYI	CCCAGC	3 cut(s)	603, 637, 848
BshFI	GGCC	2 cut(s)	189, 875
BshNI	GGYRCC	1 cut(s)	311
BshVI	ATCGAT	1 cut(s)	146
BsiHKAI	GWGCWC	2 cut(s)	210, 657
BsiHKCI	CYCGRG	1 cut(s)	203
BsiSI	CCGG	1 cut(s)	53
BslI	CCNNNNNNNGG	2 cut(s)	89, 871
BsmAI	GTCTC	1 cut(s)	809
BsmBI	CGTCTC	1 cut(s)	809
BsnI	GGCC	2 cut(s)	189, 875
BsoBI	CYCGRG	1 cut(s)	203
Bsp1286I	GDGCHC	3 cut(s)	210, 316, 657
Bsp1407I	TGTACA	4 cut(s)	140, 615, 696, 765
Bsp143I	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
Bsp1720I	GCTNAGC	1 cut(s)	599
BspACI	CCGC	7 cut(s)	23, 72, 83, 122, 186, 213, 759
BspANI	GGCC	2 cut(s)	189, 875
BspCNI	CTCAG	4 cut(s)	499, 612, 669, 859
BspDI	ATCGAT	1 cut(s)	146
BspLI	GGNNCC	2 cut(s)	313, 577
BspMAI	CTGCAG	1 cut(s)	463
BspPI	GGATC	4 cut(s)	100, 475, 511, 680
BspQI	GCTCTTC	1 cut(s)	5
BspT107I	GGYRCC	1 cut(s)	311
BsrDI	GCAATG	1 cut(s)	444
BsrFI	RCCGGY	1 cut(s)	52
BsrGI	TGTACA	4 cut(s)	140, 615, 696, 765
BsrI	ACTGG	1 cut(s)	577
BssAI	RCCGGY	1 cut(s)	52
BssECI	CCNNGG	4 cut(s)	193, 223, 373, 678
BssMI	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
BssNI	GRCGYC	1 cut(s)	791
Bst2UI	CCWGG	2 cut(s)	375, 812
Bst4CI	ACNGT	5 cut(s)	311, 679, 690, 738, 770
Bst6I	CTCTTC	1 cut(s)	5
BstACI	GRCGYC	1 cut(s)	791
BstAPI	GCANNNNNTGC	1 cut(s)	311
BstAUI	TGTACA	4 cut(s)	140, 615, 696, 765
BstBAI	YACGTR	1 cut(s)	729
BstC8I	GCNNGC	8 cut(s)	17, 21, 28, 58, 210, 750, 846, 873
BstDEI	CTNAG	4 cut(s)	486, 599, 656, 867
BstDSI	CCRYGG	1 cut(s)	678
BstF5I	GGATG	1 cut(s)	237
BstHHI	GCGC	1 cut(s)	281
BstKTI	GATC	7 cut(s)	95, 150, 353, 483, 492, 519, 675
BstMAI	GTCTC	1 cut(s)	809
BstMBI	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
BstMWI	GCNNNNNNNGC	6 cut(s)	53, 127, 234, 311, 455, 557
BstNI	CCWGG	2 cut(s)	375, 812
BstNSI	RCATGY	1 cut(s)	19
BstSCI	CCNGG	2 cut(s)	373, 810
BstSFI	CTRYAG	1 cut(s)	459
BstSLI	GKGCMC	1 cut(s)	316
BstV1I	GCAGC	1 cut(s)	445
BstV2I	GAAGAC	1 cut(s)	394
BstX2I	RGATCY	1 cut(s)	489
BstYI	RGATCY	1 cut(s)	489
Bsu15I	ATCGAT	1 cut(s)	146
BsuRI	GGCC	2 cut(s)	189, 875
BsuTUI	ATCGAT	1 cut(s)	146
BtgI	CCRYGG	1 cut(s)	678
BtrI	CACGTC	1 cut(s)	819
BtsCI	GGATG	1 cut(s)	237
BtsI	GCAGTG	1 cut(s)	288
BtsIMutI	CAGTG	1 cut(s)	288
Cac8I	GCNNGC	8 cut(s)	17, 21, 28, 58, 210, 750, 846, 873
CfoI	GCGC	1 cut(s)	281
Cfr10I	RCCGGY	1 cut(s)	52
Cfr13I	GGNCC	1 cut(s)	874
ClaI	ATCGAT	1 cut(s)	146
CseI	GACGC	2 cut(s)	633, 780
Csp6I	GTAC	9 cut(s)	40, 141, 322, 380, 472, 616, 697, 766, 823
CviAII	CATG	6 cut(s)	16, 160, 166, 529, 666, 802
CviQI	GTAC	9 cut(s)	40, 141, 322, 380, 472, 616, 697, 766, 823
DdeI	CTNAG	4 cut(s)	486, 599, 656, 867
DpnI	GATC	7 cut(s)	94, 149, 352, 482, 491, 518, 674
DpnII	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
EaeI	YGGCCR	1 cut(s)	187
Eam1104I	CTCTTC	1 cut(s)	5
EarI	CTCTTC	1 cut(s)	5
Ecl136II	GAGCTC	1 cut(s)	208
Eco24I	GRGCYC	1 cut(s)	210
Eco53kI	GAGCTC	1 cut(s)	208
Eco57I	CTGAAG	1 cut(s)	438
Eco72I	CACGTG	1 cut(s)	729
Eco88I	CYCGRG	1 cut(s)	203
EcoICRI	GAGCTC	1 cut(s)	208
EcoRII	CCWGG	2 cut(s)	373, 810
EcoT38I	GRGCYC	1 cut(s)	210
Esp3I	CGTCTC	1 cut(s)	809
FaeI	CATG	6 cut(s)	19, 163, 169, 532, 669, 805
FatI	CATG	6 cut(s)	15, 159, 165, 528, 665, 801
FauI	CCCGC	1 cut(s)	90
Fnu4HI	GCNGC	6 cut(s)	24, 73, 122, 187, 213, 459
FokI	GGATG	1 cut(s)	224
FriOI	GRGCYC	1 cut(s)	210
Fsp4HI	GCNGC	6 cut(s)	24, 73, 122, 187, 213, 459
FspBI	CTAG	1 cut(s)	581
GlaI	GCGC	1 cut(s)	280
GluI	GCNGC	6 cut(s)	24, 73, 122, 187, 213, 459
GsaI	CCCAGC	3 cut(s)	607, 641, 852
GsuI	CTGGAG	1 cut(s)	833
HaeIII	GGCC	2 cut(s)	189, 875
HapII	CCGG	1 cut(s)	53
HgaI	GACGC	2 cut(s)	633, 780
HhaI	GCGC	1 cut(s)	281
Hin1I	GRCGYC	1 cut(s)	791
Hin1II	CATG	6 cut(s)	19, 163, 169, 532, 669, 805
Hin6I	GCGC	1 cut(s)	279
HinP1I	GCGC	1 cut(s)	279
HincII	GTYRAC	1 cut(s)	508
HindII	GTYRAC	1 cut(s)	508
HindIII	AAGCTT	1 cut(s)	750
HinfI	GANTC	4 cut(s)	97, 434, 832, 881
HpaII	CCGG	1 cut(s)	53
HphI	GGTGA	3 cut(s)	379, 443, 667
Hpy166II	GTNNAC	9 cut(s)	141, 171, 299, 380, 472, 508, 616, 697, 732
Hpy188I	TCNGA	4 cut(s)	409, 441, 489, 831
Hpy188III	TCNNGA	3 cut(s)	7, 203, 514
Hpy8I	GTNNAC	9 cut(s)	141, 171, 299, 380, 472, 508, 616, 697, 732
Hpy99I	CGWCG	2 cut(s)	115, 649
HpyCH4III	ACNGT	5 cut(s)	311, 679, 690, 738, 770
HpyCH4IV	ACGT	4 cut(s)	399, 501, 728, 818
HpyCH4V	TGCA	3 cut(s)	19, 461, 532
HpyF10VI	GCNNNNNNNGC	6 cut(s)	53, 127, 234, 311, 455, 557
HpyF3I	CTNAG	4 cut(s)	486, 599, 656, 867
HpySE526I	ACGT	4 cut(s)	399, 501, 728, 818
Hsp92I	GRCGYC	1 cut(s)	791
Hsp92II	CATG	6 cut(s)	19, 163, 169, 532, 669, 805
HspAI	GCGC	1 cut(s)	279
Kzo9I	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
LguI	GCTCTTC	1 cut(s)	5
LmnI	GCTCC	1 cut(s)	110
Lsp1109I	GCAGC	1 cut(s)	445
LweI	GCATC	1 cut(s)	246
MaeI	CTAG	1 cut(s)	581
MaeII	ACGT	4 cut(s)	399, 501, 728, 818
MaeIII	GTNAC	1 cut(s)	710
MalI	GATC	7 cut(s)	94, 149, 352, 482, 491, 518, 674
MboI	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
MboII	GAAGA	4 cut(s)	22, 238, 394, 444
MflI	RGATCY	1 cut(s)	489
MhlI	GDGCHC	3 cut(s)	210, 316, 657
MluCI	AATT	1 cut(s)	620
MlyI	GAGTC	2 cut(s)	106, 875
MseI	TTAA	2 cut(s)	77, 705
MslI	CAYNNNNRTG	4 cut(s)	136, 164, 176, 680
MspA1I	CMGCKG	4 cut(s)	72, 458, 603, 759
MspI	CCGG	1 cut(s)	53
MspR9I	CCNGG	2 cut(s)	375, 812
MvaI	CCWGG	2 cut(s)	375, 812
MwoI	GCNNNNNNNGC	6 cut(s)	53, 127, 234, 311, 455, 557
NdeII	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
NlaIII	CATG	6 cut(s)	19, 163, 169, 532, 669, 805
NlaIV	GGNNCC	2 cut(s)	313, 577
NmeAIII	GCCGAG	1 cut(s)	204
NmuCI	GTSAC	1 cut(s)	710
NspI	RCATGY	1 cut(s)	19
OliI	CACNNNNGTG	1 cut(s)	680
PaeI	GCATGC	1 cut(s)	19
PaeR7I	CTCGAG	1 cut(s)	203
PciSI	GCTCTTC	1 cut(s)	5
PcsI	WCGNNNNNNNCGW	3 cut(s)	143, 201, 405
PfeI	GAWTC	2 cut(s)	434, 832
PkrI	GCNGC	6 cut(s)	25, 74, 123, 188, 214, 460
PleI	GAGTC	2 cut(s)	105, 875
PmaCI	CACGTG	1 cut(s)	729
PmlI	CACGTG	1 cut(s)	729
PpsI	GAGTC	2 cut(s)	105, 875
Ppu21I	YACGTR	1 cut(s)	729
Psp124BI	GAGCTC	1 cut(s)	210
Psp6I	CCWGG	2 cut(s)	373, 810
PspCI	CACGTG	1 cut(s)	729
PspFI	CCCAGC	3 cut(s)	603, 637, 848
PspGI	CCWGG	2 cut(s)	373, 810
PspN4I	GGNNCC	2 cut(s)	313, 577
PspPI	GGNCC	1 cut(s)	874
PstI	CTGCAG	1 cut(s)	463
PsuI	RGATCY	1 cut(s)	489
PvuII	CAGCTG	2 cut(s)	458, 603
RsaI	GTAC	9 cut(s)	41, 142, 323, 381, 473, 617, 698, 767, 824
RsaNI	GTAC	9 cut(s)	40, 141, 322, 380, 472, 616, 697, 766, 823
RseI	CAYNNNNRTG	4 cut(s)	136, 164, 176, 680
SacI	GAGCTC	1 cut(s)	210
SapI	GCTCTTC	1 cut(s)	5
SaqAI	TTAA	2 cut(s)	77, 705
SatI	GCNGC	6 cut(s)	24, 73, 122, 187, 213, 459
Sau3AI	GATC	7 cut(s)	92, 147, 350, 480, 489, 516, 672
Sau96I	GGNCC	1 cut(s)	874
ScaI	AGTACT	1 cut(s)	41
SchI	GAGTC	2 cut(s)	106, 875
ScrFI	CCNGG	2 cut(s)	375, 812
SduI	GDGCHC	3 cut(s)	210, 316, 657
SfaNI	GCATC	1 cut(s)	246
SfcI	CTRYAG	1 cut(s)	459
Sfr274I	CTCGAG	1 cut(s)	203
SlaI	CTCGAG	1 cut(s)	203
SmiMI	CAYNNNNRTG	4 cut(s)	136, 164, 176, 680
SmlI	CTYRAG	1 cut(s)	203
SmoI	CTYRAG	1 cut(s)	203
SphI	GCATGC	1 cut(s)	19
Sse9I	AATT	1 cut(s)	620
SsiI	CCGC	7 cut(s)	23, 72, 83, 122, 186, 213, 759
SspMI	CTAG	1 cut(s)	581
SstI	GAGCTC	1 cut(s)	210
StyD4I	CCNGG	2 cut(s)	373, 810
TaaI	ACNGT	5 cut(s)	311, 679, 690, 738, 770
TaiI	ACGT	4 cut(s)	402, 504, 731, 821
TaqI	TCGA	4 cut(s)	95, 146, 204, 569
TasI	AATT	1 cut(s)	620
TatI	WGTACW	6 cut(s)	39, 140, 379, 615, 696, 765
TauI	GCSGC	5 cut(s)	26, 75, 124, 189, 215
TfiI	GAWTC	2 cut(s)	434, 832
Tru1I	TTAA	2 cut(s)	77, 705
Tru9I	TTAA	2 cut(s)	77, 705
TscAI	CASTG	1 cut(s)	288
TseFI	GTSAC	1 cut(s)	710
TseI	GCWGC	1 cut(s)	458
Tsp45I	GTSAC	1 cut(s)	710
TspDTI	ATGAA	1 cut(s)	517
TspGWI	ACGGA	1 cut(s)	489
TspRI	CASTG	1 cut(s)	288
XceI	RCATGY	1 cut(s)	19
XhoI	CTCGAG	1 cut(s)	203
XspI	CTAG	1 cut(s)	581
ZrmI	AGTACT	1 cut(s)	41

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.