Rw5G002370

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001481
Physical Location & Seq
Reverse (-)
2066003 .. 2083404
17402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002370.1

Sequence Viewer

Length: 1464 bp
ATGGACTACTCCAGTGATAGTAATGAGCTCTCTGAACCCATCTTGACTGACAAAAGATCTTCATCTGTTCAACTAGTGAGCTCGGAACTGGAAGAAACGCTATCCAACACCGAGCTTCCGTACTTCCACCGTCTTAGACGTGCCACGTGGGTCGAACTGAAGATTCTCTTCCGCCTTGCTGCTCCGGCAGTGGTGGTTTACTTGCTAAACAACGTTATTTCCATGTCCACCCAAATCTACTGCTGGCATCTCGGCAATCTCGAACTCGTTAGATCAAAAGTGGACTCATCACAACTTGGAATGGGAAGTGCGGTGGAAACGCTCTGTGGTCAAGCCTACGGAGCAAAGAAATATGACATGCTAGGCGTATATCTGCAGCGTTCAACGATCCTGCTAATGGCAACTGGGATCCCAGTTATGTTCATCTACATCTTCTCCCATCTTGATTGCCCTAGGCGAATCGGCCACCATTGCCTCTTCAGCCGCAATCTTCGTTTACGGACTTATCCCCCAAATCTTTGCCTACGCTTGCAATTTCCCCATACAAAAATTCCTCCAGGGCTCCAGGCTCAGAGCATCGTGTTCCCGAGCGCGTACATATCTGCGGCTGCCCTCGTGGTGCTTGTGGTGCTGAGTTGGGTAGTGATTTGTAAGTTCGGATGGGGCTTGTTAGGCGCATCGCTGATGTTGAGCTTTTCGTGGTGGATTATAGTGGTGGCGCAGTTTGTTTACATATTGCTGAGTCCAAGGTGTAAACGTACTTGGACAGGTTTTAGCATGCAGGCTTTTTCTGGGCTTTGGAGTTTTCTGAAGCTATCTGTTGCGTCTGCCGTAATGCTTTGCCTCGAGACTTGGTACTATCAGATTATCGTTTTGATTGCTGGGTTGCTTGAAGACGCTGAAATTGCCTTGGACGCTCTCTCTGTTTGCTTGACCATATCTGGATGGGTGTTCATGGTTGCTGTGGGTTTCAATGCTGCTGCAAGTGTGAGGATTGGGAATGAGCTCGGGGCGGGACATCCAAAGTCGGCCGCATTTGCAGTAGTGTGTGCCATAGTTGTGCTTGCACTACGAAATGTCATAAGCTACGCGTTCACCGAGGGCGAGACTGTGTCCAATGCAGTCTCAGCCCTATCTCCTTACCTTGCTGTCTCTATCATACTCAACGGCATCCAACCTGTTTTGTCTGGGGTGGCTGTTGGGTGTGGATGGCAAGCATTTGTGGCATATGTTAACGTCGGATGTTACTACATTATTGGTATTCCTTTGGGTTGTGTTCTTGGCTTCAAGTTCGACTTTGGAGCCCAGACGGTGATCTTATTGTGGGTCACACTTCGGACTGATTGGAATAAAGAGTGTTCCGAGGTAATCGCACCCATTCATTGGGTTTGTCTGAGTAAAGTGTCCGTGAGTATACATAGTAATCAACCCAATCTATTCGTACAGAACATAACAGCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

487

Amino Acids

53.7

Weight (kDa)

8.3

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 99 - 147 1.5e-11 MatE
MatE PF01554 276 - 424 2.6e-23 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1383
AccI GTMKAC 1 cut(s) 1414
AccII CGCG 2 cut(s) 593, 1091
AciI CCGC 6 cut(s) 172, 311, 484, 605, 1013, 1032
AclI AACGTT 1 cut(s) 213
AclWI GGATC 3 cut(s) 382, 403, 416
AcoI YGGCCR 2 cut(s) 463, 1029
AcsI RAATTY 1 cut(s) 549
AcuI CTGAAG 3 cut(s) 179, 463, 830
AcvI CACGTG 1 cut(s) 147
AfaI GTAC 5 cut(s) 122, 596, 760, 857, 1443
AfiI CCNNNNNNNGG 2 cut(s) 397, 1383
AflIII ACRYGT 1 cut(s) 1089
AgsI TTSAA 5 cut(s) 71, 384, 893, 973, 1288
AhlI ACTAGT 1 cut(s) 73
AjiI CACGTC 1 cut(s) 140
AjnI CCWGG 2 cut(s) 556, 564
AluBI AGCT 7 cut(s) 28, 81, 115, 693, 814, 1006, 1086
AluI AGCT 7 cut(s) 28, 81, 115, 693, 814, 1006, 1086
Alw21I GWGCWC 3 cut(s) 30, 83, 1008
Alw26I GTCTC 4 cut(s) 842, 1100, 1129, 1156
AlwI GGATC 3 cut(s) 382, 403, 416
Ama87I CYCGRG 3 cut(s) 586, 845, 1007
AoxI GGCC 2 cut(s) 463, 1029
ApeKI GCWGC 5 cut(s) 179, 376, 608, 977, 980
ApoI RAATTY 1 cut(s) 549
AspA2I CCTAGG 1 cut(s) 452
AspLEI GCGC 3 cut(s) 593, 677, 721
AsuHPI GGTGA 2 cut(s) 1087, 1324
AvaI CYCGRG 3 cut(s) 586, 845, 1007
AvrII CCTAGG 1 cut(s) 452
BamHI GGATCC 1 cut(s) 408
BanII GRGCYC 5 cut(s) 30, 83, 564, 1008, 1306
BauI CACGAG 1 cut(s) 614
BbrPI CACGTG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 900
Bbv12I GWGCWC 3 cut(s) 30, 83, 1008
BbvI GCAGC 5 cut(s) 166, 388, 595, 964, 967
BccI CCATC 5 cut(s) 47, 447, 654, 939, 1203
BceAI ACGGC 2 cut(s) 815, 1183
BciT130I CCWGG 2 cut(s) 558, 566
BcoDI GTCTC 4 cut(s) 842, 1100, 1129, 1156
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 3 cut(s) 74, 362, 453
BfmI CTRYAG 1 cut(s) 374
BglII AGATCT 1 cut(s) 56
BisI GCNGC 8 cut(s) 180, 377, 484, 606, 609, 978, 981, 1032
BlnI CCTAGG 1 cut(s) 452
BlsI GCNGC 8 cut(s) 181, 378, 485, 607, 610, 979, 982, 1033
Bme1390I CCNGG 2 cut(s) 558, 566
BmeT110I CYCGRG 3 cut(s) 586, 845, 1007
BmgBI CACGTC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 410, 563, 1303
BmrFI CCNGG 2 cut(s) 558, 566
BmrI ACTGGG 2 cut(s) 407, 414
BmsI GCATC 4 cut(s) 256, 585, 686, 1179
BmuI ACTGGG 2 cut(s) 407, 414
BpiI GAAGAC 1 cut(s) 900
BpmI CTGGAG 2 cut(s) 540, 548
BsaAI YACGTR 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 6 cut(s) 452, 557, 746, 909, 1098, 1362
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 397, 1383
Bse1I ACTGG 4 cut(s) 12, 93, 409, 413
Bse3DI GCAATG 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 61
BseBI CCWGG 2 cut(s) 558, 566
BseDI CCNNGG 6 cut(s) 452, 557, 746, 909, 1098, 1362
BseGI GGATG 6 cut(s) 665, 950, 1018, 1170, 1214, 1247
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 397, 1383
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 5 cut(s) 584, 623, 731, 1140, 1385
BseNI ACTGG 4 cut(s) 12, 93, 409, 413
BseX3I CGGCCG 1 cut(s) 1029
BseXI GCAGC 5 cut(s) 166, 388, 595, 964, 967
BseYI CCCAGC 1 cut(s) 881
Bsh1236I CGCG 2 cut(s) 593, 1091
Bsh1285I CGRYCG 1 cut(s) 1032
BshFI GGCC 2 cut(s) 465, 1031
BsiEI CGRYCG 1 cut(s) 1032
BsiHKAI GWGCWC 3 cut(s) 30, 83, 1008
BsiHKCI CYCGRG 3 cut(s) 586, 845, 1007
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 1029
BslI CCNNNNNNNGG 2 cut(s) 397, 1383
BsmAI GTCTC 4 cut(s) 842, 1100, 1129, 1156
BsmFI GGGAC 1 cut(s) 1029
BsnI GGCC 2 cut(s) 465, 1031
BsoBI CYCGRG 3 cut(s) 586, 845, 1007
Bsp1286I GDGCHC 5 cut(s) 30, 83, 564, 1008, 1306
Bsp143I GATC 5 cut(s) 56, 272, 387, 408, 1314
BspACI CCGC 6 cut(s) 172, 311, 484, 605, 1013, 1032
BspANI GGCC 2 cut(s) 465, 1031
BspCNI CTCAG 5 cut(s) 583, 624, 732, 1139, 1386
BspFNI CGCG 2 cut(s) 593, 1091
BspLI GGNNCC 3 cut(s) 410, 563, 1303
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 3 cut(s) 382, 403, 416
BsrDI GCAATG 1 cut(s) 469
BsrI ACTGG 4 cut(s) 12, 93, 409, 413
BssECI CCNNGG 6 cut(s) 452, 557, 746, 909, 1098, 1362
BssMI GATC 5 cut(s) 56, 272, 387, 408, 1314
BssNAI GTATAC 1 cut(s) 1415
BssSI CACGAG 1 cut(s) 614
BssT1I CCWWGG 3 cut(s) 452, 746, 909
Bst1107I GTATAC 1 cut(s) 1415
Bst2BI CACGAG 1 cut(s) 614
Bst2UI CCWGG 2 cut(s) 558, 566
Bst4CI ACNGT 3 cut(s) 131, 1111, 1312
Bst6I CTCTTC 2 cut(s) 173, 482
BstBAI YACGTR 1 cut(s) 147
BstC8I GCNNGC 6 cut(s) 245, 530, 779, 783, 1065, 1215
BstDEI CTNAG 6 cut(s) 134, 570, 632, 740, 1126, 1394
BstF5I GGATG 6 cut(s) 665, 950, 1018, 1170, 1214, 1247
BstFNI CGCG 2 cut(s) 593, 1091
BstHHI GCGC 3 cut(s) 593, 677, 721
BstKTI GATC 5 cut(s) 59, 275, 390, 411, 1317
BstMAI GTCTC 4 cut(s) 842, 1100, 1129, 1156
BstMBI GATC 5 cut(s) 56, 272, 387, 408, 1314
BstMCI CGRYCG 1 cut(s) 1032
BstNI CCWGG 2 cut(s) 558, 566
BstNSI RCATGY 2 cut(s) 361, 781
BstSCI CCNGG 2 cut(s) 556, 564
BstSFI CTRYAG 1 cut(s) 374
BstUI CGCG 2 cut(s) 593, 1091
BstV1I GCAGC 5 cut(s) 166, 388, 595, 964, 967
BstV2I GAAGAC 1 cut(s) 900
BstX2I RGATCY 2 cut(s) 56, 408
BstYI RGATCY 2 cut(s) 56, 408
BstZ17I GTATAC 1 cut(s) 1415
BstZI CGGCCG 1 cut(s) 1029
BsuRI GGCC 2 cut(s) 465, 1031
BtgZI GCGATG 1 cut(s) 663
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 6 cut(s) 665, 950, 1018, 1170, 1214, 1247
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 19, 195
Cac8I GCNNGC 6 cut(s) 245, 530, 779, 783, 1065, 1215
CfoI GCGC 3 cut(s) 593, 677, 721
CseI GACGC 3 cut(s) 813, 905, 923
Csp6I GTAC 5 cut(s) 121, 595, 759, 856, 1442
CviAII CATG 5 cut(s) 223, 358, 778, 955, 1458
CviQI GTAC 5 cut(s) 121, 595, 759, 856, 1442
DdeI CTNAG 6 cut(s) 134, 570, 632, 740, 1126, 1394
DpnI GATC 5 cut(s) 58, 274, 389, 410, 1316
DpnII GATC 5 cut(s) 56, 272, 387, 408, 1314
EaeI YGGCCR 2 cut(s) 463, 1029
EagI CGGCCG 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 173, 482
EarI CTCTTC 2 cut(s) 173, 482
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 3 cut(s) 28, 81, 1006
EclXI CGGCCG 1 cut(s) 1029
Eco130I CCWWGG 3 cut(s) 452, 746, 909
Eco24I GRGCYC 5 cut(s) 30, 83, 564, 1008, 1306
Eco52I CGGCCG 1 cut(s) 1029
Eco53kI GAGCTC 3 cut(s) 28, 81, 1006
Eco57I CTGAAG 3 cut(s) 179, 463, 830
Eco72I CACGTG 1 cut(s) 147
Eco88I CYCGRG 3 cut(s) 586, 845, 1007
EcoICRI GAGCTC 3 cut(s) 28, 81, 1006
EcoRII CCWGG 2 cut(s) 556, 564
EcoT14I CCWWGG 3 cut(s) 452, 746, 909
EcoT38I GRGCYC 5 cut(s) 30, 83, 564, 1008, 1306
ErhI CCWWGG 3 cut(s) 452, 746, 909
FaeI CATG 5 cut(s) 226, 361, 781, 958, 1461
FalI AAGNNNNNCTT 4 cut(s) 152, 184, 1280, 1312
FaqI GGGAC 1 cut(s) 1029
FatI CATG 5 cut(s) 222, 357, 777, 954, 1457
FauI CCCGC 1 cut(s) 1006
FauNDI CATATG 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 1414
Fnu4HI GCNGC 8 cut(s) 180, 377, 484, 606, 609, 978, 981, 1032
FokI GGATG 6 cut(s) 672, 957, 1005, 1157, 1221, 1254
FriOI GRGCYC 5 cut(s) 30, 83, 564, 1008, 1306
Fsp4HI GCNGC 8 cut(s) 180, 377, 484, 606, 609, 978, 981, 1032
FspBI CTAG 3 cut(s) 74, 362, 453
GlaI GCGC 3 cut(s) 592, 676, 720
GluI GCNGC 8 cut(s) 180, 377, 484, 606, 609, 978, 981, 1032
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 2 cut(s) 540, 548
HaeIII GGCC 2 cut(s) 465, 1031
HapII CCGG 1 cut(s) 185
HgaI GACGC 3 cut(s) 813, 905, 923
HhaI GCGC 3 cut(s) 593, 677, 721
Hin1II CATG 5 cut(s) 226, 361, 781, 958, 1461
Hin6I GCGC 3 cut(s) 591, 675, 719
HinP1I GCGC 3 cut(s) 591, 675, 719
HincII GTYRAC 1 cut(s) 1234
HindII GTYRAC 1 cut(s) 1234
HinfI GANTC 4 cut(s) 163, 284, 459, 742
HpaI GTTAAC 1 cut(s) 1234
HpaII CCGG 1 cut(s) 185
HphI GGTGA 2 cut(s) 1087, 1324
Hpy166II GTNNAC 9 cut(s) 199, 228, 283, 497, 730, 755, 1095, 1234, 1415
Hpy188III TCNNGA 6 cut(s) 43, 260, 443, 586, 847, 942
Hpy8I GTNNAC 9 cut(s) 199, 228, 283, 497, 730, 755, 1095, 1234, 1415
Hpy99I CGWCG 1 cut(s) 1241
HpyCH4III ACNGT 3 cut(s) 131, 1111, 1312
HpyCH4IV ACGT 5 cut(s) 139, 146, 213, 757, 1236
HpyCH4V TGCA 7 cut(s) 376, 532, 781, 983, 1040, 1067, 1121
HpyF3I CTNAG 6 cut(s) 134, 570, 632, 740, 1126, 1394
HpySE526I ACGT 5 cut(s) 139, 146, 213, 757, 1236
Hsp92II CATG 5 cut(s) 226, 361, 781, 958, 1461
HspAI GCGC 3 cut(s) 591, 675, 719
KspAI GTTAAC 1 cut(s) 1234
Kzo9I GATC 5 cut(s) 56, 272, 387, 408, 1314
LmnI GCTCC 4 cut(s) 187, 341, 567, 1301
Lsp1109I GCAGC 5 cut(s) 166, 388, 595, 964, 967
LweI GCATC 4 cut(s) 256, 585, 686, 1179
MaeI CTAG 3 cut(s) 74, 362, 453
MaeII ACGT 5 cut(s) 139, 146, 213, 757, 1236
MaeIII GTNAC 2 cut(s) 1244, 1327
MalI GATC 5 cut(s) 58, 274, 389, 410, 1316
MboI GATC 5 cut(s) 56, 272, 387, 408, 1314
MboII GAAGA 8 cut(s) 51, 104, 160, 172, 424, 469, 482, 905
MflI RGATCY 2 cut(s) 56, 408
MhlI GDGCHC 5 cut(s) 30, 83, 564, 1008, 1306
MluCI AATT 3 cut(s) 533, 549, 903
MluI ACGCGT 1 cut(s) 1089
MlyI GAGTC 2 cut(s) 278, 751
MmeI TCCRAC 3 cut(s) 129, 1198, 1219
MnlI CCTC 7 cut(s) 485, 564, 623, 854, 984, 1093, 1357
MseI TTAA 1 cut(s) 1233
MslI CAYNNNNRTG 1 cut(s) 1058
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 2 cut(s) 558, 566
MvaI CCWGG 2 cut(s) 558, 566
MvnI CGCG 2 cut(s) 593, 1091
NdeI CATATG 1 cut(s) 1228
NdeII GATC 5 cut(s) 56, 272, 387, 408, 1314
NlaIII CATG 5 cut(s) 226, 361, 781, 958, 1461
NlaIV GGNNCC 3 cut(s) 410, 563, 1303
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 1327
NspI RCATGY 2 cut(s) 361, 781
PaeI GCATGC 1 cut(s) 781
PaeR7I CTCGAG 1 cut(s) 845
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 1095
PfeI GAWTC 2 cut(s) 163, 459
PflFI GACNNNGTC 1 cut(s) 1111
PflMI CCANNNNNTGG 1 cut(s) 1383
PkrI GCNGC 8 cut(s) 181, 378, 485, 607, 610, 979, 982, 1033
PleI GAGTC 2 cut(s) 278, 750
PmaCI CACGTG 1 cut(s) 147
PmlI CACGTG 1 cut(s) 147
PpsI GAGTC 2 cut(s) 278, 750
Ppu21I YACGTR 1 cut(s) 147
Psp124BI GAGCTC 3 cut(s) 30, 83, 1008
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 556, 564
PspCI CACGTG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 2 cut(s) 556, 564
PspN4I GGNNCC 3 cut(s) 410, 563, 1303
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 2 cut(s) 56, 408
PsyI GACNNNGTC 1 cut(s) 1111
RsaI GTAC 5 cut(s) 122, 596, 760, 857, 1443
RsaNI GTAC 5 cut(s) 121, 595, 759, 856, 1442
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 3 cut(s) 30, 83, 1008
SaqAI TTAA 1 cut(s) 1233
SatI GCNGC 8 cut(s) 180, 377, 484, 606, 609, 978, 981, 1032
Sau3AI GATC 5 cut(s) 56, 272, 387, 408, 1314
SchI GAGTC 2 cut(s) 278, 751
ScrFI CCNGG 2 cut(s) 558, 566
SduI GDGCHC 5 cut(s) 30, 83, 564, 1008, 1306
SfaNI GCATC 4 cut(s) 256, 585, 686, 1179
SfcI CTRYAG 1 cut(s) 374
Sfr274I CTCGAG 1 cut(s) 845
SlaI CTCGAG 1 cut(s) 845
SmiMI CAYNNNNRTG 1 cut(s) 1058
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SpeI ACTAGT 1 cut(s) 73
SphI GCATGC 1 cut(s) 781
Sse9I AATT 3 cut(s) 533, 549, 903
SsiI CCGC 6 cut(s) 172, 311, 484, 605, 1013, 1032
SspMI CTAG 3 cut(s) 74, 362, 453
SstI GAGCTC 3 cut(s) 30, 83, 1008
StyD4I CCNGG 2 cut(s) 556, 564
StyI CCWWGG 3 cut(s) 452, 746, 909
TaaI ACNGT 3 cut(s) 131, 1111, 1312
TaiI ACGT 5 cut(s) 142, 149, 216, 760, 1239
TaqI TCGA 4 cut(s) 153, 261, 846, 1293
TasI AATT 3 cut(s) 533, 549, 903
TauI GCSGC 3 cut(s) 486, 608, 1034
TfiI GAWTC 2 cut(s) 163, 459
Tru1I TTAA 1 cut(s) 1233
Tru9I TTAA 1 cut(s) 1233
TscAI CASTG 2 cut(s) 19, 195
TseFI GTSAC 1 cut(s) 1327
TseI GCWGC 5 cut(s) 179, 376, 608, 977, 980
Tsp45I GTSAC 1 cut(s) 1327
TspDTI ATGAA 4 cut(s) 51, 412, 943, 1370
TspGWI ACGGA 4 cut(s) 108, 354, 514, 1396
TspRI CASTG 2 cut(s) 19, 195
Tth111I GACNNNGTC 1 cut(s) 1111
Van91I CCANNNNNTGG 1 cut(s) 1383
XapI RAATTY 1 cut(s) 549
XceI RCATGY 2 cut(s) 361, 781
XhoI CTCGAG 1 cut(s) 845
XmaJI CCTAGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 1414
XspI CTAG 3 cut(s) 74, 362, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.