Rh5BG028900

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001481
Physical Location & Seq
Forward (+)
2033975 .. 2038986
5012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG028900.1

Sequence Viewer

Length: 1530 bp
ATGTTCCAGTTTATTTGTTTGAGTTTGAATGAGTTTATTATTAATTTATTTTATTTAAGGTTACTTGGTGCGCTAAGCACATATATCAGCACACCATATATATGTAGTATAAAAGCAGGTAATGGGATTCTGACAGTGGAAGTACTAAGAGAATTAAAGAAGGTCGAGAGAACTTGGGTAGATAGCATGGACTACTCCAGTGATAGTAATGAGCTCTCTGAACCCATCTTGACTGACAAAAGATCTTCATCTGTTCAACTAGTGAGCTCGGAACTGGAAGAAACGCTCTCCAGCACCGAGCTTCCGTACTTCCACCGTCTTAGACGTGCCACGTGGGTCGAACTGAAGATTCTCTTCCGCCTTGCAGCTCCGGCAGTGGTGGTTTACTTGCTAAACAACGTTATTTCCATGTCCAGCCAAATCTACTGCGGGCATCTCGGCAATCTCGAACTCGCTGCCTCTTCTCTTGGCAACACCGGCATCCAAGTTTTTGCCTACGGCCTCATGCTTGGAATGGGAAGTGCGGTGGAAACGCTCTGTGGTCAAGCCTATGGAGCAAAGAAATATGACATGCTAGGCGTATATCTGCAGCGTTCAACGATCCTGCTAATGGCAACTGGGATCCCAGTTATGTTCATCTACATCTTCTCCAAGCCCATCTTGATTGCCCTAGGCGAATCGGCCACCATTGCCTCTGCAGCCGCAATCTTTGTTTACGGACTTATCCCCCAAATCTTTGCCTACGCTTGCAATTTCCCCATACAAAAATTCCTCCAGGCTCAGAGCATCGTGTTCCCGAGCGCGTACATATCCGCGGCTGCCCTCGTGGTGCATGTGGTGCTGAGTTGGGTAGTGATTTATAAGTTGGGATGGGGCTTGTTAGGCGCATCGCTGATGTTGAGCTTTTCTTGGTGGATTATAGTGGTGGCGCAGTTTGTTTACATATTGCTGAGTCCAAGGTGTAAACGTACTTGGACAGGTTTTAGCATGCAGGCTTTTTCTGGGCTTTGGACTTTTCTGAAGCTATCTGTTGCGTCTGCCGTAATGCTTTGCCTCGAGACTTGGTACTATCAGATTATCGTTTTGATTGCTGGGTTGCTTGAAGACGCTGAAATTGCCTTGGACGCTCTCTCTGTTTGCATGACCATATCTGGATGGGTGTTCATGGTTGCTGTGGGTTTCAATGCTGCTGCAAGTGTGAGGATTGGGAATGAGCTTGGGGCGGGACATCCAAAGTCGGCCGCATTTGCAGTAATAATAGTGACATCAACCTCGTTCATCATCTCAGTAGTGTGTGCCATAGTTGTGCTTGCACTACGAAATGTCATAAGCTACGCGTTCACCGAGGGCGAGACTGTGTCCAATGCAGTCTCAGCCCTATCTCCTTACCTTGCTGTCTCTATTATGCTCAACGGCATCCAACCTGTTTTGTCTGGTACGTCTCATGCAGATTCTAGCTATATATCGGTTTCCTTATGTTTTTCTTGGTCAGTTGTTGTTTTTCCCCCAATTTTTATTATGAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

509

Amino Acids

55.54

Weight (kDa)

6.53

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 124 - 284 3e-35 MatE
MatE PF01554 346 - 481 2.3e-23 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 861
Acc36I ACCTGC 1 cut(s) 107
AccII CGCG 3 cut(s) 803, 815, 1337
AciI CCGC 8 cut(s) 358, 429, 524, 702, 813, 815, 1223, 1242
AclI AACGTT 1 cut(s) 399
AclWI GGATC 3 cut(s) 595, 616, 629
AcoI YGGCCR 2 cut(s) 681, 1239
AcsI RAATTY 1 cut(s) 767
AcuI CTGAAG 2 cut(s) 365, 1040
AcvI CACGTG 1 cut(s) 333
AfaI GTAC 6 cut(s) 144, 308, 806, 970, 1067, 1438
AfiI CCNNNNNNNGG 1 cut(s) 610
AflIII ACRYGT 1 cut(s) 1335
AgsI TTSAA 5 cut(s) 28, 257, 597, 1103, 1183
AhlI ACTAGT 1 cut(s) 259
AjiI CACGTC 1 cut(s) 326
AjnI CCWGG 1 cut(s) 774
AluBI AGCT 9 cut(s) 214, 267, 301, 368, 903, 1024, 1216, 1332, 1458
AluI AGCT 9 cut(s) 214, 267, 301, 368, 903, 1024, 1216, 1332, 1458
Alw21I GWGCWC 2 cut(s) 216, 269
Alw26I GTCTC 5 cut(s) 1052, 1346, 1375, 1402, 1446
AlwI GGATC 3 cut(s) 595, 616, 629
Ama87I CYCGRG 2 cut(s) 796, 1055
AoxI GGCC 3 cut(s) 499, 681, 1239
ApeKI GCWGC 7 cut(s) 365, 455, 589, 698, 818, 1187, 1190
ApoI RAATTY 1 cut(s) 767
AseI ATTAAT 1 cut(s) 42
AspA2I CCTAGG 1 cut(s) 670
AspLEI GCGC 4 cut(s) 73, 803, 887, 931
AsuHPI GGTGA 1 cut(s) 1333
AvaI CYCGRG 2 cut(s) 796, 1055
AvrII CCTAGG 1 cut(s) 670
BamHI GGATCC 1 cut(s) 621
BanII GRGCYC 2 cut(s) 216, 269
BauI CACGAG 1 cut(s) 824
BbrPI CACGTG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 1110
Bbv12I GWGCWC 2 cut(s) 216, 269
BbvI GCAGC 7 cut(s) 377, 442, 601, 710, 805, 1174, 1177
BccI CCATC 4 cut(s) 233, 665, 864, 1149
BceAI ACGGC 3 cut(s) 514, 1025, 1429
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 1 cut(s) 776
BcoDI GTCTC 5 cut(s) 1052, 1346, 1375, 1402, 1446
BcuI ACTAGT 1 cut(s) 259
BfaI CTAG 4 cut(s) 260, 575, 671, 1455
BfmI CTRYAG 2 cut(s) 587, 696
BfuAI ACCTGC 1 cut(s) 107
BglII AGATCT 1 cut(s) 242
BlnI CCTAGG 1 cut(s) 670
BlpI GCTNAGC 1 cut(s) 74
BmcAI AGTACT 1 cut(s) 144
Bme1390I CCNGG 1 cut(s) 776
BmeT110I CYCGRG 2 cut(s) 796, 1055
BmgBI CACGTC 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 1 cut(s) 776
BmrI ACTGGG 2 cut(s) 620, 627
BmsI GCATC 5 cut(s) 442, 489, 795, 896, 1425
BmuI ACTGGG 2 cut(s) 620, 627
BpiI GAAGAC 1 cut(s) 1110
BpmI CTGGAG 3 cut(s) 181, 274, 758
Bpu1102I GCTNAGC 1 cut(s) 74
BsaAI YACGTR 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 247
BsaJI CCNNGG 5 cut(s) 670, 813, 956, 1119, 1344
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 5 cut(s) 7, 198, 279, 622, 626
Bse3DI GCAATG 1 cut(s) 687
Bse8I GATNNNNATC 1 cut(s) 247
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 5 cut(s) 670, 813, 956, 1119, 1344
BseGI GGATG 5 cut(s) 480, 875, 1160, 1228, 1416
BseJI GATNNNNATC 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 5 cut(s) 794, 833, 941, 1299, 1386
BseNI ACTGG 5 cut(s) 7, 198, 279, 622, 626
BseX3I CGGCCG 1 cut(s) 1239
BseXI GCAGC 7 cut(s) 377, 442, 601, 710, 805, 1174, 1177
BseYI CCCAGC 1 cut(s) 1091
Bsh1236I CGCG 3 cut(s) 803, 815, 1337
Bsh1285I CGRYCG 1 cut(s) 1242
BshFI GGCC 3 cut(s) 501, 683, 1241
BsiEI CGRYCG 1 cut(s) 1242
BsiHKAI GWGCWC 2 cut(s) 216, 269
BsiHKCI CYCGRG 2 cut(s) 796, 1055
BsiSI CCGG 2 cut(s) 371, 477
BslFI GGGAC 1 cut(s) 1239
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 5 cut(s) 1052, 1346, 1375, 1402, 1446
BsmBI CGTCTC 1 cut(s) 1446
BsmFI GGGAC 1 cut(s) 1239
BsnI GGCC 3 cut(s) 501, 683, 1241
BsoBI CYCGRG 2 cut(s) 796, 1055
Bsp1286I GDGCHC 2 cut(s) 216, 269
Bsp143I GATC 3 cut(s) 242, 600, 621
Bsp1720I GCTNAGC 1 cut(s) 74
BspACI CCGC 8 cut(s) 358, 429, 524, 702, 813, 815, 1223, 1242
BspANI GGCC 3 cut(s) 501, 683, 1241
BspCNI CTCAG 5 cut(s) 793, 834, 942, 1298, 1385
BspFNI CGCG 3 cut(s) 803, 815, 1337
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 2 cut(s) 591, 700
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 3 cut(s) 595, 616, 629
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 1 cut(s) 476
BsrI ACTGG 5 cut(s) 7, 198, 279, 622, 626
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 5 cut(s) 670, 813, 956, 1119, 1344
BssMI GATC 3 cut(s) 242, 600, 621
BssSI CACGAG 1 cut(s) 824
BssT1I CCWWGG 3 cut(s) 670, 956, 1119
Bst2BI CACGAG 1 cut(s) 824
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 3 cut(s) 136, 317, 1357
Bst6I CTCTTC 2 cut(s) 359, 466
BstAPI GCANNNNNTGC 1 cut(s) 838
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 5 cut(s) 431, 748, 989, 993, 1311
BstDEI CTNAG 8 cut(s) 74, 146, 320, 780, 842, 950, 1285, 1372
BstDSI CCRYGG 1 cut(s) 813
BstF5I GGATG 5 cut(s) 480, 875, 1160, 1228, 1416
BstFNI CGCG 3 cut(s) 803, 815, 1337
BstHHI GCGC 4 cut(s) 73, 803, 887, 931
BstKTI GATC 3 cut(s) 245, 603, 624
BstMAI GTCTC 5 cut(s) 1052, 1346, 1375, 1402, 1446
BstMBI GATC 3 cut(s) 242, 600, 621
BstMCI CGRYCG 1 cut(s) 1242
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 3 cut(s) 574, 836, 991
BstSCI CCNGG 1 cut(s) 774
BstSFI CTRYAG 2 cut(s) 587, 696
BstUI CGCG 3 cut(s) 803, 815, 1337
BstV1I GCAGC 7 cut(s) 377, 442, 601, 710, 805, 1174, 1177
BstV2I GAAGAC 1 cut(s) 1110
BstX2I RGATCY 2 cut(s) 242, 621
BstYI RGATCY 2 cut(s) 242, 621
BstZI CGGCCG 1 cut(s) 1239
BsuRI GGCC 3 cut(s) 501, 683, 1241
BtgI CCRYGG 1 cut(s) 813
BtgZI GCGATG 1 cut(s) 873
BtrI CACGTC 1 cut(s) 326
BtsCI GGATG 5 cut(s) 480, 875, 1160, 1228, 1416
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 3 cut(s) 141, 205, 381
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 5 cut(s) 431, 748, 989, 993, 1311
CfoI GCGC 4 cut(s) 73, 803, 887, 931
Cfr10I RCCGGY 1 cut(s) 476
Cfr42I CCGCGG 1 cut(s) 816
CseI GACGC 3 cut(s) 1023, 1115, 1133
Csp6I GTAC 6 cut(s) 143, 307, 805, 969, 1066, 1437
CviAII CATG 9 cut(s) 187, 409, 505, 571, 833, 988, 1141, 1165, 1445
CviQI GTAC 6 cut(s) 143, 307, 805, 969, 1066, 1437
DdeI CTNAG 8 cut(s) 74, 146, 320, 780, 842, 950, 1285, 1372
DpnI GATC 3 cut(s) 244, 602, 623
DpnII GATC 3 cut(s) 242, 600, 621
EaeI YGGCCR 2 cut(s) 681, 1239
EagI CGGCCG 1 cut(s) 1239
Eam1104I CTCTTC 2 cut(s) 359, 466
EarI CTCTTC 2 cut(s) 359, 466
EciI GGCGGA 1 cut(s) 347
Ecl136II GAGCTC 2 cut(s) 214, 267
EclXI CGGCCG 1 cut(s) 1239
Eco130I CCWWGG 3 cut(s) 670, 956, 1119
Eco24I GRGCYC 2 cut(s) 216, 269
Eco52I CGGCCG 1 cut(s) 1239
Eco53kI GAGCTC 2 cut(s) 214, 267
Eco57I CTGAAG 2 cut(s) 365, 1040
Eco72I CACGTG 1 cut(s) 333
Eco88I CYCGRG 2 cut(s) 796, 1055
EcoICRI GAGCTC 2 cut(s) 214, 267
EcoRII CCWGG 1 cut(s) 774
EcoT14I CCWWGG 3 cut(s) 670, 956, 1119
EcoT38I GRGCYC 2 cut(s) 216, 269
ErhI CCWWGG 3 cut(s) 670, 956, 1119
Esp3I CGTCTC 1 cut(s) 1446
FaeI CATG 9 cut(s) 190, 412, 508, 574, 836, 991, 1144, 1168, 1448
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 644, 676
FaqI GGGAC 1 cut(s) 1239
FatI CATG 9 cut(s) 186, 408, 504, 570, 832, 987, 1140, 1164, 1444
FauI CCCGC 2 cut(s) 422, 1216
FokI GGATG 5 cut(s) 467, 882, 1167, 1215, 1403
FriOI GRGCYC 2 cut(s) 216, 269
FspBI CTAG 4 cut(s) 260, 575, 671, 1455
GlaI GCGC 4 cut(s) 72, 802, 886, 930
GsaI CCCAGC 1 cut(s) 1095
GsuI CTGGAG 3 cut(s) 181, 274, 758
HaeIII GGCC 3 cut(s) 501, 683, 1241
HapII CCGG 2 cut(s) 371, 477
HgaI GACGC 3 cut(s) 1023, 1115, 1133
HhaI GCGC 4 cut(s) 73, 803, 887, 931
Hin1II CATG 9 cut(s) 190, 412, 508, 574, 836, 991, 1144, 1168, 1448
Hin6I GCGC 4 cut(s) 71, 801, 885, 929
HinP1I GCGC 4 cut(s) 71, 801, 885, 929
HinfI GANTC 5 cut(s) 127, 349, 677, 952, 1451
HpaII CCGG 2 cut(s) 371, 477
HphI GGTGA 1 cut(s) 1333
Hpy166II GTNNAC 5 cut(s) 385, 715, 940, 965, 1341
Hpy188I TCNGA 6 cut(s) 132, 220, 271, 783, 1020, 1074
Hpy188III TCNNGA 7 cut(s) 166, 229, 446, 661, 796, 1057, 1152
Hpy8I GTNNAC 5 cut(s) 385, 715, 940, 965, 1341
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 3 cut(s) 136, 317, 1357
HpyCH4IV ACGT 5 cut(s) 325, 332, 399, 967, 1439
HpyF3I CTNAG 8 cut(s) 74, 146, 320, 780, 842, 950, 1285, 1372
HpySE526I ACGT 5 cut(s) 325, 332, 399, 967, 1439
Hsp92II CATG 9 cut(s) 190, 412, 508, 574, 836, 991, 1144, 1168, 1448
HspAI GCGC 4 cut(s) 71, 801, 885, 929
KspI CCGCGG 1 cut(s) 816
Kzo9I GATC 3 cut(s) 242, 600, 621
LmnI GCTCC 2 cut(s) 373, 554
Lsp1109I GCAGC 7 cut(s) 377, 442, 601, 710, 805, 1174, 1177
LweI GCATC 5 cut(s) 442, 489, 795, 896, 1425
MaeI CTAG 4 cut(s) 260, 575, 671, 1455
MaeII ACGT 5 cut(s) 325, 332, 399, 967, 1439
MaeIII GTNAC 2 cut(s) 60, 1261
MalI GATC 3 cut(s) 244, 602, 623
MboI GATC 3 cut(s) 242, 600, 621
MboII GAAGA 7 cut(s) 237, 290, 346, 358, 453, 637, 1115
MflI RGATCY 2 cut(s) 242, 621
MhlI GDGCHC 2 cut(s) 216, 269
MluCI AATT 6 cut(s) 43, 152, 751, 767, 1113, 1509
MluI ACGCGT 1 cut(s) 1335
MlyI GAGTC 1 cut(s) 961
MmeI TCCRAC 1 cut(s) 1444
MnlI CCTC 9 cut(s) 469, 512, 703, 782, 833, 1064, 1194, 1282, 1339
MseI TTAA 4 cut(s) 42, 56, 155, 1528
MslI CAYNNNNRTG 2 cut(s) 100, 1304
MspA1I CMGCKG 1 cut(s) 815
MspI CCGG 2 cut(s) 371, 477
MspR9I CCNGG 1 cut(s) 776
MvaI CCWGG 1 cut(s) 776
MvnI CGCG 3 cut(s) 803, 815, 1337
NdeII GATC 3 cut(s) 242, 600, 621
NlaIII CATG 9 cut(s) 190, 412, 508, 574, 836, 991, 1144, 1168, 1448
NlaIV GGNNCC 1 cut(s) 623
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 1261
NspI RCATGY 3 cut(s) 574, 836, 991
PaeI GCATGC 1 cut(s) 991
PaeR7I CTCGAG 1 cut(s) 1055
PcsI WCGNNNNNNNCGW 2 cut(s) 444, 1341
PfeI GAWTC 4 cut(s) 127, 349, 677, 1451
PflFI GACNNNGTC 1 cut(s) 1357
PleI GAGTC 1 cut(s) 960
PmaCI CACGTG 1 cut(s) 333
PmlI CACGTG 1 cut(s) 333
PpsI GAGTC 1 cut(s) 960
Ppu21I YACGTR 1 cut(s) 333
PshBI ATTAAT 1 cut(s) 42
PsiI TTATAA 1 cut(s) 861
Psp124BI GAGCTC 2 cut(s) 216, 269
Psp1406I AACGTT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 774
PspCI CACGTG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 1091
PspGI CCWGG 1 cut(s) 774
PspN4I GGNNCC 1 cut(s) 623
PstI CTGCAG 2 cut(s) 591, 700
PsuI RGATCY 2 cut(s) 242, 621
PsyI GACNNNGTC 1 cut(s) 1357
RsaI GTAC 6 cut(s) 144, 308, 806, 970, 1067, 1438
RsaNI GTAC 6 cut(s) 143, 307, 805, 969, 1066, 1437
RseI CAYNNNNRTG 2 cut(s) 100, 1304
SacI GAGCTC 2 cut(s) 216, 269
SacII CCGCGG 1 cut(s) 816
SaqAI TTAA 4 cut(s) 42, 56, 155, 1528
Sau3AI GATC 3 cut(s) 242, 600, 621
ScaI AGTACT 1 cut(s) 144
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 2 cut(s) 216, 269
SfaNI GCATC 5 cut(s) 442, 489, 795, 896, 1425
SfcI CTRYAG 2 cut(s) 587, 696
Sfr274I CTCGAG 1 cut(s) 1055
Sfr303I CCGCGG 1 cut(s) 816
SgrBI CCGCGG 1 cut(s) 816
SlaI CTCGAG 1 cut(s) 1055
SmiMI CAYNNNNRTG 2 cut(s) 100, 1304
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
SpeI ACTAGT 1 cut(s) 259
SphI GCATGC 1 cut(s) 991
Sse9I AATT 6 cut(s) 43, 152, 751, 767, 1113, 1509
SsiI CCGC 8 cut(s) 358, 429, 524, 702, 813, 815, 1223, 1242
SspMI CTAG 4 cut(s) 260, 575, 671, 1455
SstI GAGCTC 2 cut(s) 216, 269
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 3 cut(s) 670, 956, 1119
TaaI ACNGT 3 cut(s) 136, 317, 1357
TaiI ACGT 5 cut(s) 328, 335, 402, 970, 1442
TaqI TCGA 4 cut(s) 165, 339, 447, 1056
TasI AATT 6 cut(s) 43, 152, 751, 767, 1113, 1509
TatI WGTACW 1 cut(s) 142
TauI GCSGC 3 cut(s) 704, 818, 1244
TfiI GAWTC 4 cut(s) 127, 349, 677, 1451
Tru1I TTAA 4 cut(s) 42, 56, 155, 1528
Tru9I TTAA 4 cut(s) 42, 56, 155, 1528
TscAI CASTG 3 cut(s) 141, 205, 381
TseFI GTSAC 1 cut(s) 1261
TseI GCWGC 7 cut(s) 365, 455, 589, 698, 818, 1187, 1190
Tsp45I GTSAC 1 cut(s) 1261
TspDTI ATGAA 4 cut(s) 237, 625, 1153, 1267
TspGWI ACGGA 2 cut(s) 294, 732
TspRI CASTG 3 cut(s) 141, 205, 381
Tth111I GACNNNGTC 1 cut(s) 1357
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 1 cut(s) 767
XceI RCATGY 3 cut(s) 574, 836, 991
XhoI CTCGAG 1 cut(s) 1055
XmaJI CCTAGG 1 cut(s) 670
XspI CTAG 4 cut(s) 260, 575, 671, 1455
ZrmI AGTACT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.