pycom05g31380

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001481
Physical Location & Seq
Reverse (-)
31129101 .. 31133043
3943 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31380.3

Sequence Viewer

Length: 1374 bp
ATGCAACCAGAGAGTAATGCAAATTTTGTCTATAAATTTATCTCCCTAGTATTATCCTTTATCAGAACCCAGAGAGAGAGAGAGAGACTTCTTGCTAGCATGGAGTACTCAAGTGATAGTGATCTATCTAAGCCCATGTTAGTTCCCAAAACATCTTCATTACAGCAGATAGTAAGCTCGGAACTGGAAGACACACTAAACAACACTGACCTCTCAAACTTCCAGCGTCTCCGAACGGCCACGTGGCTCGAGACAAAGACCCTGTATCGCCTGGCAGCTCCGGCGGTGGTGGTGTACTTGCTCAACAATGTTATATCCATGTCCACCCAAATCTTATGTGGTCATCTTGGCAATCTTGAACTTGCTGCCTCATCTCTTGGCAACACTGGGATTCAAGTTTTCGCCTATGGCCTCATGCTTGGTATGGGTAGTGCAGTGGAAACTCTATGTGGTCAAGCCTATGGAGCACATAAATATGAAATGCTGGGCATATACATGCAGAGATCAACAATCCTCCTCGTGGCAACTGGATCGATCCCACTTATGATAATTTACATCTTCTCCAAGCCCCTCTTGCTAGCCCTAGGTGAATCAGCCTCCATCTCAGCCGCAGCCGCGATTTTCGTATACGGTCTGATCCCTCAAATATTTGCCTACGCTTGCAATTTCCCCATACAAAAATTCCTCCAAGCCCAGAGCATAGTGTTCCCTAGTGCCTACATCTCAGCAGGGGCACTTGTGGTGCACATAGTGTTAAGCTGGGTGGTGGTGTACAAACTGGATTGGGGTTTGCTGGGTGCGGCGTTGATGCTGAGCTTTTCATGGTGGATTATAGTGGTGGCACAGTTTGTGTACATTGTTTGGACTCCAAGGTGCAAGCGTACATGGAGAGGTTTTAGCATTAGGGCATTTTCCGGTCTTTGGAGATTTTTGAAGTTGTCTGCAGCATCGGCCGTAATGTTATGCCTTGAGACTTGGTATTATCAAATTATAGTTTTGATTGCTGGGTTGCTTCAAGATGCTGAAATTGCCTTGGACGCCCTCTCTATCTGCATTACCATTAGTGGATGGGTGTACATGGTTTCTGTGGGGTTCAATGCTGCTGCAAGTGTGAGGGTCGGGAATGAGCTTGGGGCTGGGCATCCAAAAGCGGCTGCATTTGCAGTTGTGATGGTAACTTCAAGCTCCTTCCTCATCTCGGTGGTCTGTGCCATAATTGTACTTGCATTACGAGATGTCATAAGTTACATTTTCACTGAGGGGGAAACAGTCTCTAATGCAGTCTCAGAACTTTCTCCTTATCTTGCAATATCTATCATTCTCAACGGTATTCAACCTGTATTGTCAGCTTCTCCTACTACTCGACATCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

458

Amino Acids

49.98

Weight (kDa)

8.38

Isoelectric Point (pI)

24.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 627
AccII CGCG 1 cut(s) 617
AciI CCGC 5 cut(s) 284, 609, 615, 800, 1151
AclWI GGATC 3 cut(s) 529, 538, 631
AcoI YGGCCR 2 cut(s) 237, 951
AcsI RAATTY 3 cut(s) 22, 35, 680
AcvI CACGTG 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 1038
AdeI CACNNNGTG 1 cut(s) 751
AfaI GTAC 7 cut(s) 107, 296, 773, 854, 883, 1076, 1221
AfiI CCNNNNNNNGG 3 cut(s) 520, 921, 1198
AgsI TTSAA 7 cut(s) 359, 395, 934, 1016, 1096, 1182, 1334
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 7 cut(s) 177, 278, 759, 816, 1129, 1185, 1349
AluI AGCT 7 cut(s) 177, 278, 759, 816, 1129, 1185, 1349
Alw21I GWGCWC 2 cut(s) 469, 747
Alw26I GTCTC 6 cut(s) 79, 233, 245, 965, 1276, 1288
Alw44I GTGCAC 1 cut(s) 743
AlwI GGATC 3 cut(s) 529, 538, 631
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 3 cut(s) 237, 409, 951
ApaLI GTGCAC 1 cut(s) 743
ApeKI GCWGC 7 cut(s) 275, 365, 611, 944, 1100, 1103, 1154
ApoI RAATTY 3 cut(s) 22, 35, 680
AspA2I CCTAGG 1 cut(s) 583
AsuHPI GGTGA 1 cut(s) 599
AsuNHI GCTAGC 2 cut(s) 95, 577
AvaI CYCGRG 1 cut(s) 248
AvrII CCTAGG 1 cut(s) 583
BaeGI GKGCMC 2 cut(s) 736, 747
BauI CACGAG 1 cut(s) 518
BbrPI CACGTG 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 2 cut(s) 469, 747
BbvI GCAGC 7 cut(s) 287, 352, 623, 956, 1087, 1090, 1141
BccI CCATC 3 cut(s) 608, 1062, 1165
BceAI ACGGC 2 cut(s) 252, 938
BcgI CGANNNNNNTGC 2 cut(s) 513, 547
BciT130I CCWGG 1 cut(s) 272
BcoDI GTCTC 6 cut(s) 79, 233, 245, 965, 1276, 1288
BfaI CTAG 5 cut(s) 47, 96, 578, 584, 711
BfmI CTRYAG 1 cut(s) 942
BlnI CCTAGG 1 cut(s) 583
BlpI GCTNAGC 1 cut(s) 812
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 4 cut(s) 798, 956, 1009, 1150
BmtI GCTAGC 2 cut(s) 99, 581
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 812
BpuEI CTTGAG 2 cut(s) 94, 989
Bsa29I ATCGAT 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 1038
BsaJI CCNNGG 3 cut(s) 583, 869, 1032
BsaWI WCCGGW 1 cut(s) 914
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 3 cut(s) 520, 921, 1198
Bse1I ACTGG 4 cut(s) 189, 391, 532, 783
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 1 cut(s) 272
BseCI ATCGAT 1 cut(s) 533
BseDI CCNNGG 3 cut(s) 583, 869, 1032
BseGI GGATG 2 cut(s) 1073, 1141
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 520, 921, 1198
BseMII CTCAG 5 cut(s) 618, 738, 803, 1248, 1299
BseNI ACTGG 4 cut(s) 189, 391, 532, 783
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 2 cut(s) 736, 747
BseX3I CGGCCG 1 cut(s) 951
BseXI GCAGC 7 cut(s) 287, 352, 623, 956, 1087, 1090, 1141
BseYI CCCAGC 5 cut(s) 484, 759, 793, 1004, 1136
BsgI GTGCAG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 617
Bsh1285I CGRYCG 1 cut(s) 954
BshFI GGCC 3 cut(s) 239, 411, 953
BshVI ATCGAT 1 cut(s) 533
BsiEI CGRYCG 1 cut(s) 954
BsiHKAI GWGCWC 2 cut(s) 469, 747
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 2 cut(s) 281, 915
BslI CCNNNNNNNGG 3 cut(s) 520, 921, 1198
BsmAI GTCTC 6 cut(s) 79, 233, 245, 965, 1276, 1288
BsmBI CGTCTC 1 cut(s) 233
BsnI GGCC 3 cut(s) 239, 411, 953
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 469, 736, 747
Bsp1407I TGTACA 3 cut(s) 771, 852, 1074
Bsp143I GATC 5 cut(s) 121, 503, 530, 534, 636
Bsp1720I GCTNAGC 1 cut(s) 812
BspACI CCGC 5 cut(s) 284, 609, 615, 800, 1151
BspANI GGCC 3 cut(s) 239, 411, 953
BspCNI CTCAG 5 cut(s) 617, 737, 804, 1249, 1298
BspDI ATCGAT 1 cut(s) 533
BspFNI CGCG 1 cut(s) 617
BspMAI CTGCAG 1 cut(s) 946
BspOI GCTAGC 2 cut(s) 99, 581
BspPI GGATC 3 cut(s) 529, 538, 631
BsrGI TGTACA 3 cut(s) 771, 852, 1074
BsrI ACTGG 4 cut(s) 189, 391, 532, 783
BssECI CCNNGG 3 cut(s) 583, 869, 1032
BssMI GATC 5 cut(s) 121, 503, 530, 534, 636
BssNAI GTATAC 1 cut(s) 628
BssNI GRCGYC 1 cut(s) 1038
BssSI CACGAG 1 cut(s) 518
BssT1I CCWWGG 3 cut(s) 583, 869, 1032
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 518
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 632, 846, 1270, 1328
BstACI GRCGYC 1 cut(s) 1038
BstAUI TGTACA 3 cut(s) 771, 852, 1074
BstBAI YACGTR 1 cut(s) 243
BstC8I GCNNGC 4 cut(s) 97, 579, 661, 878
BstDEI CTNAG 6 cut(s) 129, 604, 724, 812, 1257, 1285
BstF5I GGATG 2 cut(s) 1073, 1141
BstFNI CGCG 1 cut(s) 617
BstKTI GATC 5 cut(s) 124, 506, 533, 537, 639
BstMAI GTCTC 6 cut(s) 79, 233, 245, 965, 1276, 1288
BstMBI GATC 5 cut(s) 121, 503, 530, 534, 636
BstMCI CGRYCG 1 cut(s) 954
BstMWI GCNNNNNNNGC 8 cut(s) 281, 464, 574, 614, 950, 1028, 1037, 1160
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 499
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 2 cut(s) 736, 747
BstUI CGCG 1 cut(s) 617
BstV1I GCAGC 7 cut(s) 287, 352, 623, 956, 1087, 1090, 1141
BstV2I GAAGAC 1 cut(s) 195
BstZ17I GTATAC 1 cut(s) 628
BstZI CGGCCG 1 cut(s) 951
Bsu15I ATCGAT 1 cut(s) 533
BsuRI GGCC 3 cut(s) 239, 411, 953
BsuTUI ATCGAT 1 cut(s) 533
BtsCI GGATG 2 cut(s) 1073, 1141
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 4 cut(s) 204, 384, 441, 1254
Cac8I GCNNGC 4 cut(s) 97, 579, 661, 878
ClaI ATCGAT 1 cut(s) 533
CseI GACGC 2 cut(s) 215, 1046
Csp6I GTAC 7 cut(s) 106, 295, 772, 853, 882, 1075, 1220
CviAII CATG 8 cut(s) 100, 136, 319, 415, 496, 822, 885, 1078
CviQI GTAC 7 cut(s) 106, 295, 772, 853, 882, 1075, 1220
DdeI CTNAG 6 cut(s) 129, 604, 724, 812, 1257, 1285
DpnI GATC 5 cut(s) 123, 505, 532, 536, 638
DpnII GATC 5 cut(s) 121, 503, 530, 534, 636
DraIII CACNNNGTG 1 cut(s) 751
EaeI YGGCCR 2 cut(s) 237, 951
EagI CGGCCG 1 cut(s) 951
EclXI CGGCCG 1 cut(s) 951
Eco130I CCWWGG 3 cut(s) 583, 869, 1032
Eco52I CGGCCG 1 cut(s) 951
Eco72I CACGTG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 3 cut(s) 583, 869, 1032
ErhI CCWWGG 3 cut(s) 583, 869, 1032
Esp3I CGTCTC 1 cut(s) 233
FaeI CATG 8 cut(s) 103, 139, 322, 418, 499, 825, 888, 1081
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FatI CATG 8 cut(s) 99, 135, 318, 414, 495, 821, 884, 1077
FblI GTMKAC 1 cut(s) 627
FokI GGATG 2 cut(s) 1080, 1128
FspBI CTAG 5 cut(s) 47, 96, 578, 584, 711
GsaI CCCAGC 5 cut(s) 488, 763, 797, 1008, 1140
HaeIII GGCC 3 cut(s) 239, 411, 953
HapII CCGG 2 cut(s) 281, 915
HgaI GACGC 2 cut(s) 215, 1046
Hin1I GRCGYC 1 cut(s) 1038
Hin1II CATG 8 cut(s) 103, 139, 322, 418, 499, 825, 888, 1081
HinfI GANTC 3 cut(s) 391, 590, 865
HpaII CCGG 2 cut(s) 281, 915
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 7 cut(s) 295, 324, 628, 745, 772, 853, 1075
Hpy188I TCNGA 5 cut(s) 65, 181, 233, 636, 1288
Hpy188III TCNNGA 4 cut(s) 250, 356, 1016, 1120
Hpy8I GTNNAC 7 cut(s) 295, 324, 628, 745, 772, 853, 1075
HpyAV CCTTC 1 cut(s) 1198
HpyCH4III ACNGT 4 cut(s) 632, 846, 1270, 1328
HpyCH4IV ACGT 1 cut(s) 242
HpyF10VI GCNNNNNNNGC 8 cut(s) 281, 464, 574, 614, 950, 1028, 1037, 1160
HpyF3I CTNAG 6 cut(s) 129, 604, 724, 812, 1257, 1285
HpySE526I ACGT 1 cut(s) 242
Hsp92I GRCGYC 1 cut(s) 1038
Hsp92II CATG 8 cut(s) 103, 139, 322, 418, 499, 825, 888, 1081
Kzo9I GATC 5 cut(s) 121, 503, 530, 534, 636
LmnI GCTCC 3 cut(s) 283, 464, 1190
Lsp1109I GCAGC 7 cut(s) 287, 352, 623, 956, 1087, 1090, 1141
LweI GCATC 4 cut(s) 798, 956, 1009, 1150
MaeI CTAG 5 cut(s) 47, 96, 578, 584, 711
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 2 cut(s) 1174, 1244
MalI GATC 5 cut(s) 123, 505, 532, 536, 638
MboI GATC 5 cut(s) 121, 503, 530, 534, 636
MboII GAAGA 3 cut(s) 147, 200, 550
MhlI GDGCHC 3 cut(s) 469, 736, 747
MluCI AATT 8 cut(s) 22, 35, 549, 664, 680, 987, 1026, 1215
MlyI GAGTC 1 cut(s) 859
MseI TTAA 1 cut(s) 755
MslI CAYNNNNRTG 3 cut(s) 474, 494, 1199
MspI CCGG 2 cut(s) 281, 915
MspR9I CCNGG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 617
MwoI GCNNNNNNNGC 8 cut(s) 281, 464, 574, 614, 950, 1028, 1037, 1160
NdeII GATC 5 cut(s) 121, 503, 530, 534, 636
NheI GCTAGC 2 cut(s) 95, 577
NlaIII CATG 8 cut(s) 103, 139, 322, 418, 499, 825, 888, 1081
NspI RCATGY 1 cut(s) 499
PaeR7I CTCGAG 1 cut(s) 248
PfeI GAWTC 2 cut(s) 391, 590
PleI GAGTC 1 cut(s) 859
PmaCI CACGTG 1 cut(s) 243
PmlI CACGTG 1 cut(s) 243
PpsI GAGTC 1 cut(s) 859
Ppu21I YACGTR 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 270
PspCI CACGTG 1 cut(s) 243
PspFI CCCAGC 5 cut(s) 484, 759, 793, 1004, 1136
PspGI CCWGG 1 cut(s) 270
PstI CTGCAG 1 cut(s) 946
RsaI GTAC 7 cut(s) 107, 296, 773, 854, 883, 1076, 1221
RsaNI GTAC 7 cut(s) 106, 295, 772, 853, 882, 1075, 1220
RseI CAYNNNNRTG 3 cut(s) 474, 494, 1199
SaqAI TTAA 1 cut(s) 755
Sau3AI GATC 5 cut(s) 121, 503, 530, 534, 636
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 1 cut(s) 859
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 3 cut(s) 469, 736, 747
SfaNI GCATC 4 cut(s) 798, 956, 1009, 1150
SfcI CTRYAG 1 cut(s) 942
Sfr274I CTCGAG 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 3 cut(s) 474, 494, 1199
SmlI CTYRAG 3 cut(s) 109, 248, 968
SmoI CTYRAG 3 cut(s) 109, 248, 968
Sse9I AATT 8 cut(s) 22, 35, 549, 664, 680, 987, 1026, 1215
SsiI CCGC 5 cut(s) 284, 609, 615, 800, 1151
SspI AATATT 1 cut(s) 648
SspMI CTAG 5 cut(s) 47, 96, 578, 584, 711
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 3 cut(s) 583, 869, 1032
TaaI ACNGT 4 cut(s) 632, 846, 1270, 1328
TaiI ACGT 1 cut(s) 245
TaqI TCGA 3 cut(s) 249, 533, 1363
TasI AATT 8 cut(s) 22, 35, 549, 664, 680, 987, 1026, 1215
TatI WGTACW 6 cut(s) 105, 294, 771, 852, 1074, 1219
TauI GCSGC 4 cut(s) 611, 617, 803, 1154
TfiI GAWTC 2 cut(s) 391, 590
Tru1I TTAA 1 cut(s) 755
Tru9I TTAA 1 cut(s) 755
TscAI CASTG 4 cut(s) 211, 391, 441, 1261
TseI GCWGC 7 cut(s) 275, 365, 611, 944, 1100, 1103, 1154
TspDTI ATGAA 3 cut(s) 147, 492, 810
TspRI CASTG 4 cut(s) 211, 391, 441, 1261
VneI GTGCAC 1 cut(s) 743
XapI RAATTY 3 cut(s) 22, 35, 680
XceI RCATGY 1 cut(s) 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 335
XhoI CTCGAG 1 cut(s) 248
XmaJI CCTAGG 1 cut(s) 583
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 5 cut(s) 47, 96, 578, 584, 711
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.