RchiOBHm_Chr5g0003321

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001481
Physical Location & Seq
Forward (+)
2066234 .. 2070855
4622 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28463

Sequence Viewer

Length: 1512 bp
ATGGACTACTCCAGTGAGCTCTCTGAACCCATCTTGACTGACAAAAGATCTTCATCTGTTCAACTAGTGAGCTCGGAACTGGAAGAAACGCTATCCAACACCGAGCTTCCGTTCTTCCACCGTCTTAGACGTGCCACGTGGGTCGAACTGAAGATTCTCTGCCGCCTTGCAGCTCCGGCAGTGGTGGTTTACTTGCTAAACAACGTTATTTCCATGTCCACCCAAATCTACTGCGGGCATCTAGGCAATCTCGAACTCGCTGCCTCTTCTCTTGGCAACACCGGCATCCAAGTTTTTGCCTACGGCCTCATGCTTGGAATGGGAAGTGCGGTGGAAACGCTCTGTGGTCAAGCCTATGGAGCAAAGAAATATGACATGCTAGGCGTATATCTGCAGCGTTCAACGATCCTGCTAATGGCAACTGGGATCCCAGTTATGTTCATTTACATCTTCTCCAAGGCCATCTTGATTGCCCTAGGCGAATCGGCCACCATTGCCTCTTCAGCCGCAATCTTCGTTTACGGACTTATCCCCCAAATCTTTGCCTACGCTTGCAATTTCCCCATACAAAAATTCCTCCAGGCTCAGAGCATCGTGTTCCCGAGCGCGTACATATCTGCGGCTGCCCTCGTGGTGCATGTGGTGCTGAGTTGGGTAGTGATTTATAAGTTGGGATGGGGCTTGTTAGGCGCATCGCTGATGTTGAGCTTTTCGTGGTGGATTATAGTGGTGGCGCAGTTTGTTTACATATTGCTGAGTCCAAGGTGTAAACGTACTTGGACAGGTTTCAGCATGCAGGCTTTTTCTGGGCTTTGGAGTTTTCTGAAGCTATCTGTTGCGTCTGCCGTAATGCTTTGCCTCGAGACTTGGTACTATCAGATTATCGTTTTGATCGCTGGGTTGCTTGAAGACGCTGAAATTGCCTTGGACGCTCTCTCTGTTTGCATGACCATATCTGGATGGGTGTTCATGGTTGCTGTGGGTTTCAATGCTGCTGCAAGTGTGAGGATTGGGAATGAGCTCGGGGCGGGACATCCAAAGTCGGCCGCATTTGCAGTAATAATAGTGACATCAACCTCGTTCATCATCTCAGTAGTGTGTGCCATAGTTGTGCTTGCACTACGAAATGTCATAAGCTACGCGTTCACCGAGGGCGAGACTGTGTCCAATGCAGTCTCAGCCCTATCTCCTTACCTTGCTGTCTCTATTATGCTCAACGGCATCCAACCTGTTTTGTCTGGGGTGGCTGTTGGGTGTGGATGGCAAGCATTTGTGGCATATGTTAACGTCGGATGTTACTACATTATTGGTGTTCCTTTGGGTTGTGTTCTTGGCTTCAAGTTCGACTTTGGAGCCCAGGGAATATGGTCTGGAATGATAGGAGGGACATTTATACAGACGGTGATCTTATTGTGGGTCACACTTCGGACTGATTGGAATAAAGAGGTCGCAAATGCAAAGAATCGTTTGGACAAGTGGGATGACAAGAAAGAGCCTCTTCTCATAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

503

Amino Acids

54.63

Weight (kDa)

6.45

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 59 - 219 6.7e-34 MatE
MatE PF01554 281 - 441 9.2e-30 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AccII CGCG 2 cut(s) 608, 1142
AciI CCGC 7 cut(s) 163, 234, 329, 507, 620, 1028, 1047
AclI AACGTT 1 cut(s) 204
AclWI GGATC 3 cut(s) 400, 421, 434
AcoI YGGCCR 2 cut(s) 486, 1044
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 3 cut(s) 170, 486, 845
AcvI CACGTG 1 cut(s) 138
AfaI GTAC 3 cut(s) 611, 775, 872
AfiI CCNNNNNNNGG 1 cut(s) 415
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 5 cut(s) 62, 402, 908, 988, 1339
AhlI ACTAGT 1 cut(s) 64
AjiI CACGTC 1 cut(s) 131
AjnI CCWGG 2 cut(s) 579, 1356
AluBI AGCT 8 cut(s) 19, 72, 106, 173, 708, 829, 1021, 1137
AluI AGCT 8 cut(s) 19, 72, 106, 173, 708, 829, 1021, 1137
Alw21I GWGCWC 3 cut(s) 21, 74, 1023
Alw26I GTCTC 4 cut(s) 857, 1151, 1180, 1207
AlwI GGATC 3 cut(s) 400, 421, 434
Ama87I CYCGRG 3 cut(s) 601, 860, 1022
AoxI GGCC 4 cut(s) 304, 459, 486, 1044
ApeKI GCWGC 6 cut(s) 170, 260, 394, 623, 992, 995
ApoI RAATTY 1 cut(s) 572
AspA2I CCTAGG 1 cut(s) 475
AspLEI GCGC 3 cut(s) 608, 692, 736
AsuHPI GGTGA 2 cut(s) 1138, 1414
AvaI CYCGRG 3 cut(s) 601, 860, 1022
AvrII CCTAGG 1 cut(s) 475
BamHI GGATCC 1 cut(s) 426
BanII GRGCYC 4 cut(s) 21, 74, 1023, 1357
BauI CACGAG 1 cut(s) 629
BbrPI CACGTG 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 915
Bbv12I GWGCWC 3 cut(s) 21, 74, 1023
BbvI GCAGC 6 cut(s) 182, 247, 406, 610, 979, 982
BccI CCATC 5 cut(s) 38, 470, 669, 954, 1254
BceAI ACGGC 3 cut(s) 319, 830, 1234
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 2 cut(s) 581, 1358
BcoDI GTCTC 4 cut(s) 857, 1151, 1180, 1207
BcuI ACTAGT 1 cut(s) 64
BfaI CTAG 4 cut(s) 65, 242, 380, 476
BfmI CTRYAG 1 cut(s) 392
BglII AGATCT 1 cut(s) 47
BlnI CCTAGG 1 cut(s) 475
Bme1390I CCNGG 2 cut(s) 581, 1358
BmeT110I CYCGRG 3 cut(s) 601, 860, 1022
BmgBI CACGTC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 428, 1354
BmrFI CCNGG 2 cut(s) 581, 1358
BmrI ACTGGG 2 cut(s) 425, 432
BmsI GCATC 5 cut(s) 247, 294, 600, 701, 1230
BmuI ACTGGG 2 cut(s) 425, 432
BpiI GAAGAC 1 cut(s) 915
BpmI CTGGAG 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 52
BsaJI CCNNGG 7 cut(s) 456, 475, 761, 924, 1149, 1356, 1357
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 4 cut(s) 12, 84, 427, 431
Bse3DI GCAATG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 52
BseBI CCWGG 2 cut(s) 581, 1358
BseDI CCNNGG 7 cut(s) 456, 475, 761, 924, 1149, 1356, 1357
BseGI GGATG 8 cut(s) 285, 680, 965, 1033, 1221, 1265, 1298, 1486
BseJI GATNNNNATC 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 5 cut(s) 599, 638, 746, 1104, 1191
BseNI ACTGG 4 cut(s) 12, 84, 427, 431
BseX3I CGGCCG 1 cut(s) 1044
BseXI GCAGC 6 cut(s) 182, 247, 406, 610, 979, 982
BseYI CCCAGC 1 cut(s) 896
Bsh1236I CGCG 2 cut(s) 608, 1142
Bsh1285I CGRYCG 1 cut(s) 1047
BshFI GGCC 4 cut(s) 306, 461, 488, 1046
BsiEI CGRYCG 1 cut(s) 1047
BsiHKAI GWGCWC 3 cut(s) 21, 74, 1023
BsiHKCI CYCGRG 3 cut(s) 601, 860, 1022
BsiSI CCGG 2 cut(s) 176, 282
BslFI GGGAC 2 cut(s) 1044, 1399
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 4 cut(s) 857, 1151, 1180, 1207
BsmFI GGGAC 2 cut(s) 1044, 1399
BsnI GGCC 4 cut(s) 306, 461, 488, 1046
BsoBI CYCGRG 3 cut(s) 601, 860, 1022
Bsp1286I GDGCHC 4 cut(s) 21, 74, 1023, 1357
Bsp143I GATC 5 cut(s) 47, 405, 426, 891, 1404
BspACI CCGC 7 cut(s) 163, 234, 329, 507, 620, 1028, 1047
BspANI GGCC 4 cut(s) 306, 461, 488, 1046
BspCNI CTCAG 5 cut(s) 598, 639, 747, 1103, 1190
BspFNI CGCG 2 cut(s) 608, 1142
BspLI GGNNCC 2 cut(s) 428, 1354
BspMAI CTGCAG 1 cut(s) 396
BspPI GGATC 3 cut(s) 400, 421, 434
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 4 cut(s) 12, 84, 427, 431
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 7 cut(s) 456, 475, 761, 924, 1149, 1356, 1357
BssMI GATC 5 cut(s) 47, 405, 426, 891, 1404
BssSI CACGAG 1 cut(s) 629
BssT1I CCWWGG 4 cut(s) 456, 475, 761, 924
Bst2BI CACGAG 1 cut(s) 629
Bst2UI CCWGG 2 cut(s) 581, 1358
Bst4CI ACNGT 3 cut(s) 122, 1162, 1402
Bst6I CTCTTC 3 cut(s) 271, 505, 1503
BstAPI GCANNNNNTGC 1 cut(s) 643
BstBAI YACGTR 1 cut(s) 138
BstC8I GCNNGC 6 cut(s) 236, 553, 794, 798, 1116, 1266
BstDEI CTNAG 6 cut(s) 125, 585, 647, 755, 1090, 1177
BstF5I GGATG 8 cut(s) 285, 680, 965, 1033, 1221, 1265, 1298, 1486
BstFNI CGCG 2 cut(s) 608, 1142
BstHHI GCGC 3 cut(s) 608, 692, 736
BstKTI GATC 5 cut(s) 50, 408, 429, 894, 1407
BstMAI GTCTC 4 cut(s) 857, 1151, 1180, 1207
BstMBI GATC 5 cut(s) 47, 405, 426, 891, 1404
BstMCI CGRYCG 1 cut(s) 1047
BstNI CCWGG 2 cut(s) 581, 1358
BstNSI RCATGY 3 cut(s) 379, 641, 796
BstSCI CCNGG 2 cut(s) 579, 1356
BstSFI CTRYAG 1 cut(s) 392
BstUI CGCG 2 cut(s) 608, 1142
BstV1I GCAGC 6 cut(s) 182, 247, 406, 610, 979, 982
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 2 cut(s) 47, 426
BstYI RGATCY 2 cut(s) 47, 426
BstZI CGGCCG 1 cut(s) 1044
BsuRI GGCC 4 cut(s) 306, 461, 488, 1046
BtgZI GCGATG 1 cut(s) 678
BtrI CACGTC 1 cut(s) 131
BtsCI GGATG 8 cut(s) 285, 680, 965, 1033, 1221, 1265, 1298, 1486
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 19, 186
Cac8I GCNNGC 6 cut(s) 236, 553, 794, 798, 1116, 1266
CfoI GCGC 3 cut(s) 608, 692, 736
Cfr10I RCCGGY 1 cut(s) 281
CseI GACGC 3 cut(s) 828, 920, 938
Csp6I GTAC 3 cut(s) 610, 774, 871
CviAII CATG 7 cut(s) 214, 310, 376, 638, 793, 946, 970
CviQI GTAC 3 cut(s) 610, 774, 871
DdeI CTNAG 6 cut(s) 125, 585, 647, 755, 1090, 1177
DpnI GATC 5 cut(s) 49, 407, 428, 893, 1406
DpnII GATC 5 cut(s) 47, 405, 426, 891, 1404
EaeI YGGCCR 2 cut(s) 486, 1044
EagI CGGCCG 1 cut(s) 1044
Eam1104I CTCTTC 3 cut(s) 271, 505, 1503
EarI CTCTTC 3 cut(s) 271, 505, 1503
Ecl136II GAGCTC 3 cut(s) 19, 72, 1021
EclXI CGGCCG 1 cut(s) 1044
Eco130I CCWWGG 4 cut(s) 456, 475, 761, 924
Eco24I GRGCYC 4 cut(s) 21, 74, 1023, 1357
Eco52I CGGCCG 1 cut(s) 1044
Eco53kI GAGCTC 3 cut(s) 19, 72, 1021
Eco57I CTGAAG 3 cut(s) 170, 486, 845
Eco72I CACGTG 1 cut(s) 138
Eco88I CYCGRG 3 cut(s) 601, 860, 1022
EcoICRI GAGCTC 3 cut(s) 19, 72, 1021
EcoRII CCWGG 2 cut(s) 579, 1356
EcoT14I CCWWGG 4 cut(s) 456, 475, 761, 924
EcoT38I GRGCYC 4 cut(s) 21, 74, 1023, 1357
ErhI CCWWGG 4 cut(s) 456, 475, 761, 924
FaeI CATG 7 cut(s) 217, 313, 379, 641, 796, 949, 973
FalI AAGNNNNNCTT 5 cut(s) 449, 481, 1331, 1363, 1482
FaqI GGGAC 2 cut(s) 1044, 1399
FatI CATG 7 cut(s) 213, 309, 375, 637, 792, 945, 969
FauI CCCGC 2 cut(s) 227, 1021
FauNDI CATATG 1 cut(s) 1279
FokI GGATG 8 cut(s) 272, 687, 972, 1020, 1208, 1272, 1305, 1493
FriOI GRGCYC 4 cut(s) 21, 74, 1023, 1357
FspBI CTAG 4 cut(s) 65, 242, 380, 476
GlaI GCGC 3 cut(s) 607, 691, 735
GsaI CCCAGC 1 cut(s) 900
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 4 cut(s) 306, 461, 488, 1046
HapII CCGG 2 cut(s) 176, 282
HgaI GACGC 3 cut(s) 828, 920, 938
HhaI GCGC 3 cut(s) 608, 692, 736
Hin1II CATG 7 cut(s) 217, 313, 379, 641, 796, 949, 973
Hin6I GCGC 3 cut(s) 606, 690, 734
HinP1I GCGC 3 cut(s) 606, 690, 734
HincII GTYRAC 1 cut(s) 1285
HindII GTYRAC 1 cut(s) 1285
HinfI GANTC 4 cut(s) 154, 482, 757, 1462
HpaI GTTAAC 1 cut(s) 1285
HpaII CCGG 2 cut(s) 176, 282
HphI GGTGA 2 cut(s) 1138, 1414
Hpy166II GTNNAC 7 cut(s) 190, 219, 520, 745, 770, 1146, 1285
Hpy188I TCNGA 7 cut(s) 25, 76, 588, 825, 879, 1292, 1428
Hpy188III TCNNGA 7 cut(s) 34, 251, 466, 601, 862, 957, 1371
Hpy8I GTNNAC 7 cut(s) 190, 219, 520, 745, 770, 1146, 1285
Hpy99I CGWCG 1 cut(s) 1292
HpyCH4III ACNGT 3 cut(s) 122, 1162, 1402
HpyCH4IV ACGT 5 cut(s) 130, 137, 204, 772, 1287
HpyF3I CTNAG 6 cut(s) 125, 585, 647, 755, 1090, 1177
HpySE526I ACGT 5 cut(s) 130, 137, 204, 772, 1287
Hsp92II CATG 7 cut(s) 217, 313, 379, 641, 796, 949, 973
HspAI GCGC 3 cut(s) 606, 690, 734
KspAI GTTAAC 1 cut(s) 1285
Kzo9I GATC 5 cut(s) 47, 405, 426, 891, 1404
LmnI GCTCC 3 cut(s) 178, 359, 1352
Lsp1109I GCAGC 6 cut(s) 182, 247, 406, 610, 979, 982
LweI GCATC 5 cut(s) 247, 294, 600, 701, 1230
MaeI CTAG 4 cut(s) 65, 242, 380, 476
MaeII ACGT 5 cut(s) 130, 137, 204, 772, 1287
MaeIII GTNAC 3 cut(s) 1066, 1295, 1417
MalI GATC 5 cut(s) 49, 407, 428, 893, 1406
MboI GATC 5 cut(s) 47, 405, 426, 891, 1404
MflI RGATCY 2 cut(s) 47, 426
MhlI GDGCHC 4 cut(s) 21, 74, 1023, 1357
MluCI AATT 3 cut(s) 556, 572, 918
MluI ACGCGT 1 cut(s) 1140
MlyI GAGTC 1 cut(s) 766
MmeI TCCRAC 3 cut(s) 120, 1249, 1270
MseI TTAA 1 cut(s) 1284
MslI CAYNNNNRTG 1 cut(s) 1109
MspI CCGG 2 cut(s) 176, 282
MspR9I CCNGG 2 cut(s) 581, 1358
MvaI CCWGG 2 cut(s) 581, 1358
MvnI CGCG 2 cut(s) 608, 1142
NdeI CATATG 1 cut(s) 1279
NdeII GATC 5 cut(s) 47, 405, 426, 891, 1404
NlaIII CATG 7 cut(s) 217, 313, 379, 641, 796, 949, 973
NlaIV GGNNCC 2 cut(s) 428, 1354
NmuCI GTSAC 2 cut(s) 1066, 1417
NspI RCATGY 3 cut(s) 379, 641, 796
PaeI GCATGC 1 cut(s) 796
PaeR7I CTCGAG 1 cut(s) 860
PasI CCCWGGG 1 cut(s) 1357
PcsI WCGNNNNNNNCGW 1 cut(s) 1146
PfeI GAWTC 3 cut(s) 154, 482, 1462
PflFI GACNNNGTC 1 cut(s) 1162
PleI GAGTC 1 cut(s) 765
PmaCI CACGTG 1 cut(s) 138
PmlI CACGTG 1 cut(s) 138
PpsI GAGTC 1 cut(s) 765
Ppu21I YACGTR 1 cut(s) 138
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 3 cut(s) 21, 74, 1023
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 579, 1356
PspCI CACGTG 1 cut(s) 138
PspFI CCCAGC 1 cut(s) 896
PspGI CCWGG 2 cut(s) 579, 1356
PspN4I GGNNCC 2 cut(s) 428, 1354
PstI CTGCAG 1 cut(s) 396
PsuI RGATCY 2 cut(s) 47, 426
PsyI GACNNNGTC 1 cut(s) 1162
RsaI GTAC 3 cut(s) 611, 775, 872
RsaNI GTAC 3 cut(s) 610, 774, 871
RseI CAYNNNNRTG 1 cut(s) 1109
SacI GAGCTC 3 cut(s) 21, 74, 1023
SaqAI TTAA 1 cut(s) 1284
Sau3AI GATC 5 cut(s) 47, 405, 426, 891, 1404
SchI GAGTC 1 cut(s) 766
ScrFI CCNGG 2 cut(s) 581, 1358
SduI GDGCHC 4 cut(s) 21, 74, 1023, 1357
SfaNI GCATC 5 cut(s) 247, 294, 600, 701, 1230
SfcI CTRYAG 1 cut(s) 392
Sfr274I CTCGAG 1 cut(s) 860
SlaI CTCGAG 1 cut(s) 860
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SpeI ACTAGT 1 cut(s) 64
SphI GCATGC 1 cut(s) 796
Sse9I AATT 3 cut(s) 556, 572, 918
SsiI CCGC 7 cut(s) 163, 234, 329, 507, 620, 1028, 1047
SspMI CTAG 4 cut(s) 65, 242, 380, 476
SstI GAGCTC 3 cut(s) 21, 74, 1023
StyD4I CCNGG 2 cut(s) 579, 1356
StyI CCWWGG 4 cut(s) 456, 475, 761, 924
TaaI ACNGT 3 cut(s) 122, 1162, 1402
TaiI ACGT 5 cut(s) 133, 140, 207, 775, 1290
TaqI TCGA 4 cut(s) 144, 252, 861, 1344
TasI AATT 3 cut(s) 556, 572, 918
TauI GCSGC 4 cut(s) 165, 509, 623, 1049
TfiI GAWTC 3 cut(s) 154, 482, 1462
Tru1I TTAA 1 cut(s) 1284
Tru9I TTAA 1 cut(s) 1284
TscAI CASTG 2 cut(s) 19, 186
TseFI GTSAC 2 cut(s) 1066, 1417
TseI GCWGC 6 cut(s) 170, 260, 394, 623, 992, 995
Tsp45I GTSAC 2 cut(s) 1066, 1417
TspDTI ATGAA 4 cut(s) 42, 430, 958, 1072
TspGWI ACGGA 2 cut(s) 99, 537
TspRI CASTG 2 cut(s) 19, 186
Tth111I GACNNNGTC 1 cut(s) 1162
XapI RAATTY 1 cut(s) 572
XceI RCATGY 3 cut(s) 379, 641, 796
XhoI CTCGAG 1 cut(s) 860
XmaJI CCTAGG 1 cut(s) 475
XspI CTAG 4 cut(s) 65, 242, 380, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.