Prupe.4G021900_v2.0.a1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001481
Physical Location & Seq
Forward (+)
1038736 .. 1041925
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G021900.1

Sequence Viewer

Length: 1524 bp
ATGGAGCCTTCTAGTGATAACGAGCCCTCTGAGCCCCTCTTGCTTTCCAAATCATCTTCGATGCATATAGTGAGCCGTGAACTGGAAGACACACTAAACAACACTGAACTCTCATACTTCCAGCGCCTCCGAACCGGCACATGGCTTGAGCTGAAGATCCTCTTCCGCCTGGCAGCTCCGGCGGTGGTGGTTTACTTGCTCAACAATGTCATATCCATGTCCACCCAAATCTTCTGTGGCCATCTTGGCAACCTTGAACTCGCTGCCTCCTCTCTTGGCAACACTGGCATTCAAGTTTTCGCCTATGGCCTCATGCTTGGTATGGGTAGTGCAGTGGAGACACTGTGTGGTCAAGCCTATGGTGCACATAAGTACGACATGCTAGGCATATATATGCAGCGATCGACAATCCTCCTCCTAGCAACTAGCATCCCACTGATGATAATTTACATCTTCTGCAAGCAAATCTTGTTAGGCCTAGGTGAATCAGCCTCCATTGCTGCCGCGGCAGCGATCTTTGTCTACGGTCTCATCCCTCAAATCTTTGCCTACGCCTGCAACTTCCCCATACAAAAGTTCCTCCAAGCCCAGAGCATAGTGTTCCCTAGCGCCTACATATCGCTGGGGGCACTGGTAGTGCACATAGTGCTAAGTTGGGTGGTGGTGTACAAATTGGGAGGGGGCCTGTTGGGCGCCGCGTTGATTTTGAGTTTTTCGTGGTGGATTATAGTGGTGGCGCAATTTGTTTACATATTGTGGACGCCAAGGTGCAAACGCACATGGAGAGGGTTTAGCATCAGGGCATTTTCTGGGCTGTGGGGCTTTTTGAAATTGTCTGTGGCATCTGCCGTCATGCTTTGCCTGGAGACTTGGTACTATCAAATTATAGTTTTGCTGGCTGGGTTGCTTAAAAATGCTGAAATTGCATTGGACGCTCTCTCTATTTGCATGACCATAACTGGATGGGTCTTCATGATTGCTGTGGGGTTCAATGCTGCTGCAAGTGTGAGGATTGGAAATGAGCTTGGGGCAGGACATCCAAAATCGGCAGCATTTGCAGTGGTGGTGGTGACTTCAAGCTCCTTCATCATATCGGCGGTGTGCGCCATAATTGTGCTTGTTTTACGACATGATATAAGTTATGTGTTCACTGAGGGAGAAACAGTCTCCAATGCCGTCTCAGACCTTTCCCCGTACCTAGCCATATCTATCGTTCTCAATGGCATCCAACCTGTTTTGTCTGGAGTGGCTGTCGGGTGCGGATGGCAAGCATTTGTGGCATATGTTAACGTGGGATGTTACTACATCATTGGGATCCCATTGGGTTGCCTTCTTGGATTCAAGTTCGATTTCGGAGCTCAGGGAATATGGACTGGCATGATAGGAGGCACTTTTATACAGACAATCATCTTACTGTGGGTCACATATCGGACTGACTGGAACAAAGAGGTTGAAAAAGCACAGAGTCGTTTGGACACTTACGAGGACAAGAAGGATTCCGATGATGAAGTGCTGACCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

508

Amino Acids

55.26

Weight (kDa)

5.9

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 692
AccI GTMKAC 1 cut(s) 522
AccII CGCG 2 cut(s) 506, 698
AciI CCGC 7 cut(s) 166, 182, 504, 506, 696, 1097, 1260
AclWI GGATC 3 cut(s) 151, 1309, 1322
AcoI YGGCCR 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 173
AcyI GRCGYC 2 cut(s) 693, 761
AdeI CACNNNGTG 2 cut(s) 347, 646
AfaI GTAC 4 cut(s) 374, 668, 875, 1196
AfiI CCNNNNNNNGG 2 cut(s) 82, 141
AgsI TTSAA 7 cut(s) 257, 293, 829, 991, 1077, 1342, 1454
AjnI CCWGG 2 cut(s) 168, 861
AluBI AGCT 5 cut(s) 151, 176, 1024, 1080, 1358
AluI AGCT 5 cut(s) 151, 176, 1024, 1080, 1358
Alw21I GWGCWC 3 cut(s) 367, 642, 1360
Alw26I GTCTC 5 cut(s) 332, 533, 860, 1171, 1183
Alw44I GTGCAC 2 cut(s) 363, 638
AlwI GGATC 3 cut(s) 151, 1309, 1322
AoxI GGCC 4 cut(s) 238, 307, 475, 682
ApaLI GTGCAC 2 cut(s) 363, 638
ApeKI GCWGC 8 cut(s) 173, 263, 397, 500, 509, 995, 998, 1049
AspA2I CCTAGG 1 cut(s) 478
AspLEI GCGC 5 cut(s) 126, 611, 695, 739, 1106
AspS9I GGNCC 1 cut(s) 682
AsuHPI GGTGA 2 cut(s) 494, 1081
AvrII CCTAGG 1 cut(s) 478
BaeGI GKGCMC 3 cut(s) 367, 631, 642
BalI TGGCCA 1 cut(s) 240
BamHI GGATCC 1 cut(s) 1314
BanI GGYRCC 1 cut(s) 692
BanII GRGCYC 3 cut(s) 27, 36, 1360
BbsI GAAGAC 2 cut(s) 93, 961
Bbv12I GWGCWC 3 cut(s) 367, 642, 1360
BbvI GCAGC 8 cut(s) 185, 250, 409, 487, 521, 982, 985, 1061
BccI CCATC 3 cut(s) 249, 957, 1257
BceAI ACGGC 3 cut(s) 60, 833, 1160
BciT130I CCWGG 2 cut(s) 170, 863
BcoDI GTCTC 5 cut(s) 332, 533, 860, 1171, 1183
BfaI CTAG 7 cut(s) 12, 383, 419, 426, 479, 606, 1199
BfoI RGCGCY 3 cut(s) 127, 612, 696
BglI GCCNNNNNGGC 2 cut(s) 246, 690
BlnI CCTAGG 1 cut(s) 478
Bme1390I CCNGG 2 cut(s) 170, 863
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 4 cut(s) 6, 683, 694, 1316
BmrFI CCNGG 2 cut(s) 170, 863
BmsI GCATC 5 cut(s) 51, 438, 804, 851, 1233
BpiI GAAGAC 2 cut(s) 93, 961
BpmI CTGGAG 2 cut(s) 884, 1263
Bpu10I CCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 1 cut(s) 167
BsaHI GRCGYC 2 cut(s) 693, 761
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 3 cut(s) 478, 504, 764
BsaXI ACNNNNNCTCC 8 cut(s) 329, 359, 857, 887, 1149, 1179, 1236, 1266
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 141
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 6 cut(s) 87, 289, 636, 964, 1378, 1442
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 2 cut(s) 170, 863
BseDI CCNNGG 3 cut(s) 478, 504, 764
BseGI GGATG 7 cut(s) 429, 531, 968, 1036, 1224, 1268, 1301
BseLI CCNNNNNNNGG 2 cut(s) 82, 141
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 4 cut(s) 21, 1143, 1194, 1373
BseNI ACTGG 6 cut(s) 87, 289, 636, 964, 1378, 1442
BseRI GAGGAG 2 cut(s) 259, 404
BseSI GKGCMC 3 cut(s) 367, 631, 642
BseXI GCAGC 8 cut(s) 185, 250, 409, 487, 521, 982, 985, 1061
BseYI CCCAGC 2 cut(s) 622, 899
BsgI GTGCAG 1 cut(s) 351
Bsh1236I CGCG 2 cut(s) 506, 698
Bsh1285I CGRYCG 1 cut(s) 404
BshFI GGCC 4 cut(s) 240, 309, 477, 684
BshNI GGYRCC 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 404
BsiHKAI GWGCWC 3 cut(s) 367, 642, 1360
BsiSI CCGG 2 cut(s) 135, 179
BslI CCNNNNNNNGG 2 cut(s) 82, 141
BsmAI GTCTC 5 cut(s) 332, 533, 860, 1171, 1183
BsmBI CGTCTC 1 cut(s) 1183
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 4 cut(s) 240, 309, 477, 684
Bso31I GGTCTC 1 cut(s) 533
Bsp1286I GDGCHC 6 cut(s) 27, 36, 367, 631, 642, 1360
Bsp1407I TGTACA 1 cut(s) 666
Bsp143I GATC 4 cut(s) 156, 401, 513, 1314
BspACI CCGC 7 cut(s) 166, 182, 504, 506, 696, 1097, 1260
BspANI GGCC 4 cut(s) 240, 309, 477, 684
BspCNI CTCAG 4 cut(s) 22, 1144, 1193, 1372
BspFNI CGCG 2 cut(s) 506, 698
BspHI TCATGA 1 cut(s) 972
BspLI GGNNCC 4 cut(s) 6, 683, 694, 1316
BspPI GGATC 3 cut(s) 151, 1309, 1322
BspT107I GGYRCC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 666
BsrI ACTGG 6 cut(s) 87, 289, 636, 964, 1378, 1442
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 3 cut(s) 478, 504, 764
BssMI GATC 4 cut(s) 156, 401, 513, 1314
BssNI GRCGYC 2 cut(s) 693, 761
BssT1I CCWWGG 2 cut(s) 478, 764
Bst2UI CCWGG 2 cut(s) 170, 863
Bst4CI ACNGT 4 cut(s) 345, 527, 1165, 1416
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 2 cut(s) 693, 761
BstAPI GCANNNNNTGC 2 cut(s) 646, 1055
BstAUI TGTACA 1 cut(s) 666
BstC8I GCNNGC 4 cut(s) 461, 556, 897, 1269
BstDEI CTNAG 5 cut(s) 30, 650, 1152, 1180, 1359
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 7 cut(s) 429, 531, 968, 1036, 1224, 1268, 1301
BstFNI CGCG 2 cut(s) 506, 698
BstH2I RGCGCY 3 cut(s) 127, 612, 696
BstHHI GCGC 5 cut(s) 126, 611, 695, 739, 1106
BstKTI GATC 4 cut(s) 159, 404, 516, 1317
BstMAI GTCTC 5 cut(s) 332, 533, 860, 1171, 1183
BstMBI GATC 4 cut(s) 156, 401, 513, 1314
BstMCI CGRYCG 1 cut(s) 404
BstNI CCWGG 2 cut(s) 170, 863
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 168, 861
BstSLI GKGCMC 3 cut(s) 367, 631, 642
BstUI CGCG 2 cut(s) 506, 698
BstV1I GCAGC 8 cut(s) 185, 250, 409, 487, 521, 982, 985, 1061
BstV2I GAAGAC 2 cut(s) 93, 961
BstX2I RGATCY 2 cut(s) 156, 1314
BstYI RGATCY 2 cut(s) 156, 1314
BsuRI GGCC 4 cut(s) 240, 309, 477, 684
BtgI CCRYGG 1 cut(s) 504
BtsCI GGATG 7 cut(s) 429, 531, 968, 1036, 1224, 1268, 1301
BtsI GCAGTG 2 cut(s) 339, 1065
BtsIMutI CAGTG 8 cut(s) 102, 282, 339, 341, 434, 629, 1065, 1149
Cac8I GCNNGC 4 cut(s) 461, 556, 897, 1269
CciI TCATGA 1 cut(s) 972
CfoI GCGC 5 cut(s) 126, 611, 695, 739, 1106
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 682
Cfr42I CCGCGG 1 cut(s) 507
CseI GACGC 2 cut(s) 769, 941
Csp6I GTAC 4 cut(s) 373, 667, 874, 1195
CviQI GTAC 4 cut(s) 373, 667, 874, 1195
DdeI CTNAG 5 cut(s) 30, 650, 1152, 1180, 1359
DinI GGCGCC 1 cut(s) 694
DpnI GATC 4 cut(s) 158, 403, 515, 1316
DpnII GATC 4 cut(s) 156, 401, 513, 1314
DraIII CACNNNGTG 2 cut(s) 347, 646
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 1358
Eco130I CCWWGG 2 cut(s) 478, 764
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 3 cut(s) 27, 36, 1360
Eco31I GGTCTC 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 1358
Eco57I CTGAAG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 1358
EcoO109I RGGNCCY 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 168, 861
EcoT14I CCWWGG 2 cut(s) 478, 764
EcoT22I ATGCAT 1 cut(s) 66
EcoT38I GRGCYC 3 cut(s) 27, 36, 1360
EgeI GGCGCC 1 cut(s) 694
EheI GGCGCC 1 cut(s) 694
ErhI CCWWGG 2 cut(s) 478, 764
Esp3I CGTCTC 1 cut(s) 1183
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 452, 484
FauNDI CATATG 1 cut(s) 1282
FblI GTMKAC 1 cut(s) 522
FokI GGATG 7 cut(s) 416, 518, 975, 1023, 1211, 1275, 1308
FriOI GRGCYC 3 cut(s) 27, 36, 1360
FspBI CTAG 7 cut(s) 12, 383, 419, 426, 479, 606, 1199
GlaI GCGC 5 cut(s) 125, 610, 694, 738, 1105
GsaI CCCAGC 2 cut(s) 626, 903
GsuI CTGGAG 2 cut(s) 884, 1263
HaeII RGCGCY 3 cut(s) 127, 612, 696
HaeIII GGCC 4 cut(s) 240, 309, 477, 684
HapII CCGG 2 cut(s) 135, 179
HgaI GACGC 2 cut(s) 769, 941
HhaI GCGC 5 cut(s) 126, 611, 695, 739, 1106
Hin1I GRCGYC 2 cut(s) 693, 761
Hin6I GCGC 5 cut(s) 124, 609, 693, 737, 1104
HinP1I GCGC 5 cut(s) 124, 609, 693, 737, 1104
HincII GTYRAC 1 cut(s) 1288
HindII GTYRAC 1 cut(s) 1288
HinfI GANTC 4 cut(s) 485, 1338, 1465, 1496
HpaI GTTAAC 1 cut(s) 1288
HpaII CCGG 2 cut(s) 135, 179
HphI GGTGA 2 cut(s) 494, 1081
Hpy188I TCNGA 6 cut(s) 31, 131, 1183, 1355, 1431, 1501
Hpy188III TCNNGA 2 cut(s) 973, 1242
HpyAV CCTTC 4 cut(s) 18, 1093, 1340, 1486
HpyCH4III ACNGT 4 cut(s) 345, 527, 1165, 1416
HpyCH4IV ACGT 1 cut(s) 1290
HpyF3I CTNAG 5 cut(s) 30, 650, 1152, 1180, 1359
HpySE526I ACGT 1 cut(s) 1290
Hsp92I GRCGYC 2 cut(s) 693, 761
HspAI GCGC 5 cut(s) 124, 609, 693, 737, 1104
KasI GGCGCC 1 cut(s) 692
KspAI GTTAAC 1 cut(s) 1288
KspI CCGCGG 1 cut(s) 507
Kzo9I GATC 4 cut(s) 156, 401, 513, 1314
LmnI GCTCC 4 cut(s) 4, 181, 1085, 1355
Lsp1109I GCAGC 8 cut(s) 185, 250, 409, 487, 521, 982, 985, 1061
LweI GCATC 5 cut(s) 51, 438, 804, 851, 1233
MaeI CTAG 7 cut(s) 12, 383, 419, 426, 479, 606, 1199
MaeII ACGT 1 cut(s) 1290
MaeIII GTNAC 3 cut(s) 1069, 1298, 1420
MalI GATC 4 cut(s) 158, 403, 515, 1316
MboI GATC 4 cut(s) 156, 401, 513, 1314
MboII GAAGA 7 cut(s) 48, 98, 154, 166, 223, 445, 961
MflI RGATCY 2 cut(s) 156, 1314
MhlI GDGCHC 6 cut(s) 27, 36, 367, 631, 642, 1360
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 8 cut(s) 444, 671, 740, 830, 882, 921, 1110, 1519
MluNI TGGCCA 1 cut(s) 240
Mly113I GGCGCC 1 cut(s) 693
MlyI GAGTC 1 cut(s) 1474
MmeI TCCRAC 1 cut(s) 1252
Mox20I TGGCCA 1 cut(s) 240
Mph1103I ATGCAT 1 cut(s) 66
MscI TGGCCA 1 cut(s) 240
MseI TTAA 2 cut(s) 909, 1287
MslI CAYNNNNRTG 3 cut(s) 215, 392, 1112
Msp20I TGGCCA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 2 cut(s) 135, 179
MspR9I CCNGG 2 cut(s) 170, 863
Mva1269I GAATGC 1 cut(s) 288
MvaI CCWGG 2 cut(s) 170, 863
MvnI CGCG 2 cut(s) 506, 698
NarI GGCGCC 1 cut(s) 693
NdeI CATATG 1 cut(s) 1282
NdeII GATC 4 cut(s) 156, 401, 513, 1314
NlaIV GGNNCC 4 cut(s) 6, 683, 694, 1316
NmuCI GTSAC 2 cut(s) 1069, 1420
NsiI ATGCAT 1 cut(s) 66
NspI RCATGY 1 cut(s) 382
PagI TCATGA 1 cut(s) 972
PceI AGGCCT 1 cut(s) 477
PctI GAATGC 1 cut(s) 288
PfeI GAWTC 3 cut(s) 485, 1338, 1496
Ple19I CGATCG 1 cut(s) 404
PleI GAGTC 1 cut(s) 1473
PluTI GGCGCC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 1473
Psp124BI GAGCTC 1 cut(s) 1360
Psp6I CCWGG 2 cut(s) 168, 861
PspFI CCCAGC 2 cut(s) 622, 899
PspGI CCWGG 2 cut(s) 168, 861
PspN4I GGNNCC 4 cut(s) 6, 683, 694, 1316
PspPI GGNCC 1 cut(s) 682
PsuI RGATCY 2 cut(s) 156, 1314
PvuI CGATCG 1 cut(s) 404
RsaI GTAC 4 cut(s) 374, 668, 875, 1196
RsaNI GTAC 4 cut(s) 373, 667, 874, 1195
RseI CAYNNNNRTG 3 cut(s) 215, 392, 1112
SacI GAGCTC 1 cut(s) 1360
SacII CCGCGG 1 cut(s) 507
SaqAI TTAA 2 cut(s) 909, 1287
Sau3AI GATC 4 cut(s) 156, 401, 513, 1314
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 1 cut(s) 1474
ScrFI CCNGG 2 cut(s) 170, 863
SduI GDGCHC 6 cut(s) 27, 36, 367, 631, 642, 1360
SfaNI GCATC 5 cut(s) 51, 438, 804, 851, 1233
SfoI GGCGCC 1 cut(s) 694
Sfr303I CCGCGG 1 cut(s) 507
SgrBI CCGCGG 1 cut(s) 507
SmiMI CAYNNNNRTG 3 cut(s) 215, 392, 1112
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 8 cut(s) 444, 671, 740, 830, 882, 921, 1110, 1519
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 7 cut(s) 166, 182, 504, 506, 696, 1097, 1260
SspDI GGCGCC 1 cut(s) 692
SspMI CTAG 7 cut(s) 12, 383, 419, 426, 479, 606, 1199
SstI GAGCTC 1 cut(s) 1360
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 2 cut(s) 168, 861
StyI CCWWGG 2 cut(s) 478, 764
TaaI ACNGT 4 cut(s) 345, 527, 1165, 1416
TaiI ACGT 1 cut(s) 1293
TaqI TCGA 3 cut(s) 59, 404, 1347
TasI AATT 8 cut(s) 444, 671, 740, 830, 882, 921, 1110, 1519
TatI WGTACW 1 cut(s) 666
TauI GCSGC 3 cut(s) 506, 509, 698
TfiI GAWTC 3 cut(s) 485, 1338, 1496
Tru1I TTAA 2 cut(s) 909, 1287
Tru9I TTAA 2 cut(s) 909, 1287
TscAI CASTG 8 cut(s) 109, 289, 339, 348, 441, 636, 1065, 1156
TseFI GTSAC 2 cut(s) 1069, 1420
TseI GCWGC 8 cut(s) 173, 263, 397, 500, 509, 995, 998, 1049
Tsp45I GTSAC 2 cut(s) 1069, 1420
TspDTI ATGAA 3 cut(s) 961, 1075, 1521
TspRI CASTG 8 cut(s) 109, 289, 339, 348, 441, 636, 1065, 1156
VneI GTGCAC 2 cut(s) 363, 638
XceI RCATGY 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmaJI CCTAGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 7 cut(s) 12, 383, 419, 426, 479, 606, 1199
Zsp2I ATGCAT 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.