RchiOBHm_Chr5g0003371

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001481
Physical Location & Seq
Forward (+)
2099300 .. 2114695
15396 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28468

Sequence Viewer

Length: 813 bp
ATGTTGAGCTTTTCGTGGTGGATTATAGTGGTGGCGCAGTTTGTTTACATATTGCTGAGTCCAAGGTGTAAACGTACTTGGACAGGTTTTAGCATGCAGGCTTTTTCTGGGCTTTGGAGTTTTCTGAAGCTATCTGTTGCGTCTGCCGTAATTCTTTGCCTCGAGACTTGGTACTATCAGATTATCGTTTTGATTGCTGGGTTGCTTGAAGACGCTGAAATTGCCTTGGACGCTCTCTCTGTTTGCATGACCATATCTGGATGGGTGTTCATGGTTTCTGTGGGTTTCAATGCTGCTGCAAGTGTGAGGATTGGGAATGAGCTCGGGGCGGGACATCCAAAGTCGGCTGCATTTGCAGTAATAATAGTGACATCAACCTCGTTCCTCATCTCTGTAGTGTGTGCCATTGTTGTGCTTGCACTACGAAATGTCATAAGCTACGCGTTCACCGAGGGCAAGACTGTGTCCAATGCAGTTTCAGCCCTATCTTCTTACCTTGCTGTCTCTATTATGCTCAACGGCATCCAACCTGTTTTGTCTGGGGTGGCTGTTGGGTGTGGATGGCAAGCATTTGTGGCATATGTTAACGTCGGATGTTACTACATTATTGGTATTCCTTTGGGTTGTGTTCTTGGCTTTAAGTTCGACTTTGGAGCCCACGGAATATGGTCTGGAATGATAGGAGGGACATTTATACAGACGGTGATCTTATTGTGGGTCACACTTCGGACTGATTGGAATAAAGAGGTCGCAAATGCAAAGAATCGTTTGGACAAGTGGGATGACAAGAAAGAGCCTCTTCTCATAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

270

Amino Acids

29.36

Weight (kDa)

7.53

Isoelectric Point (pI)

19.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 48 - 208 1.2e-29 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 443
AciI CCGC 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 146
AfaI GTAC 2 cut(s) 76, 173
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 2 cut(s) 209, 289
AluBI AGCT 4 cut(s) 9, 130, 322, 438
AluI AGCT 4 cut(s) 9, 130, 322, 438
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 2 cut(s) 158, 508
Ama87I CYCGRG 2 cut(s) 161, 323
ApeKI GCWGC 3 cut(s) 293, 296, 347
AspLEI GCGC 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 439, 715
AvaI CYCGRG 2 cut(s) 161, 323
BanII GRGCYC 2 cut(s) 324, 658
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 3 cut(s) 280, 283, 334
BccI CCATC 2 cut(s) 255, 555
BceAI ACGGC 2 cut(s) 131, 535
BcoDI GTCTC 2 cut(s) 158, 508
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 3 cut(s) 294, 297, 348
BlsI GCNGC 3 cut(s) 295, 298, 349
BmeT110I CYCGRG 2 cut(s) 161, 323
BmiI GGNNCC 1 cut(s) 655
BmsI GCATC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 216
BsaJI CCNNGG 4 cut(s) 62, 225, 450, 658
BseDI CCNNGG 4 cut(s) 62, 225, 450, 658
BseGI GGATG 6 cut(s) 266, 334, 522, 566, 599, 787
BseMII CTCAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 280, 283, 334
BseYI CCCAGC 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 443
BsiHKAI GWGCWC 1 cut(s) 324
BsiHKCI CYCGRG 2 cut(s) 161, 323
BslFI GGGAC 2 cut(s) 345, 700
BsmAI GTCTC 2 cut(s) 158, 508
BsmFI GGGAC 2 cut(s) 345, 700
BsoBI CYCGRG 2 cut(s) 161, 323
Bsp1286I GDGCHC 2 cut(s) 324, 658
Bsp143I GATC 1 cut(s) 705
BspACI CCGC 1 cut(s) 329
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 1 cut(s) 443
BspLI GGNNCC 1 cut(s) 655
BssECI CCNNGG 4 cut(s) 62, 225, 450, 658
BssMI GATC 1 cut(s) 705
BssT1I CCWWGG 2 cut(s) 62, 225
Bst4CI ACNGT 2 cut(s) 463, 703
Bst6I CTCTTC 1 cut(s) 804
BstC8I GCNNGC 4 cut(s) 95, 99, 417, 567
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 6 cut(s) 266, 334, 522, 566, 599, 787
BstFNI CGCG 1 cut(s) 443
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 1 cut(s) 708
BstMAI GTCTC 2 cut(s) 158, 508
BstMBI GATC 1 cut(s) 705
BstMWI GCNNNNNNNGC 5 cut(s) 221, 230, 353, 479, 575
BstNSI RCATGY 1 cut(s) 97
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 1 cut(s) 443
BstV1I GCAGC 3 cut(s) 280, 283, 334
BstV2I GAAGAC 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 6 cut(s) 266, 334, 522, 566, 599, 787
Cac8I GCNNGC 4 cut(s) 95, 99, 417, 567
CfoI GCGC 1 cut(s) 37
CseI GACGC 3 cut(s) 129, 221, 239
Csp6I GTAC 2 cut(s) 75, 172
CviAII CATG 3 cut(s) 94, 247, 271
CviQI GTAC 2 cut(s) 75, 172
DdeI CTNAG 1 cut(s) 56
DpnI GATC 1 cut(s) 707
DpnII GATC 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 62, 225
Eco24I GRGCYC 2 cut(s) 324, 658
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 146
Eco88I CYCGRG 2 cut(s) 161, 323
EcoICRI GAGCTC 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 62, 225
EcoT38I GRGCYC 2 cut(s) 324, 658
ErhI CCWWGG 2 cut(s) 62, 225
FaeI CATG 3 cut(s) 97, 250, 274
FalI AAGNNNNNCTT 3 cut(s) 632, 664, 783
FaqI GGGAC 2 cut(s) 345, 700
FatI CATG 3 cut(s) 93, 246, 270
FauI CCCGC 1 cut(s) 322
FauNDI CATATG 1 cut(s) 580
Fnu4HI GCNGC 3 cut(s) 294, 297, 348
FokI GGATG 6 cut(s) 273, 321, 509, 573, 606, 794
FriOI GRGCYC 2 cut(s) 324, 658
Fsp4HI GCNGC 3 cut(s) 294, 297, 348
GlaI GCGC 1 cut(s) 36
GluI GCNGC 3 cut(s) 294, 297, 348
GsaI CCCAGC 1 cut(s) 201
HgaI GACGC 3 cut(s) 129, 221, 239
HhaI GCGC 1 cut(s) 37
Hin1II CATG 3 cut(s) 97, 250, 274
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 2 cut(s) 58, 763
HpaI GTTAAC 1 cut(s) 586
HphI GGTGA 2 cut(s) 439, 715
Hpy166II GTNNAC 4 cut(s) 46, 71, 447, 586
Hpy188I TCNGA 4 cut(s) 126, 180, 593, 729
Hpy188III TCNNGA 3 cut(s) 163, 258, 672
Hpy8I GTNNAC 4 cut(s) 46, 71, 447, 586
Hpy99I CGWCG 1 cut(s) 593
HpyCH4III ACNGT 2 cut(s) 463, 703
HpyCH4IV ACGT 2 cut(s) 73, 588
HpyCH4V TGCA 8 cut(s) 97, 246, 299, 350, 356, 419, 473, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 230, 353, 479, 575
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 2 cut(s) 73, 588
Hsp92II CATG 3 cut(s) 97, 250, 274
HspAI GCGC 1 cut(s) 35
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 1 cut(s) 705
LmnI GCTCC 1 cut(s) 653
LpnPI CCDG 8 cut(s) 69, 83, 93, 183, 243, 525, 543, 657
Lsp1109I GCAGC 3 cut(s) 280, 283, 334
LweI GCATC 1 cut(s) 531
MaeII ACGT 2 cut(s) 73, 588
MaeIII GTNAC 3 cut(s) 367, 596, 718
MalI GATC 1 cut(s) 707
MboI GATC 1 cut(s) 705
MboII GAAGA 3 cut(s) 221, 480, 791
MhlI GDGCHC 2 cut(s) 324, 658
MluCI AATT 2 cut(s) 150, 219
MluI ACGCGT 1 cut(s) 441
MlyI GAGTC 1 cut(s) 67
MmeI TCCRAC 2 cut(s) 550, 571
MnlI CCTC 8 cut(s) 170, 300, 388, 395, 445, 677, 739, 807
MseI TTAA 2 cut(s) 585, 639
MslI CAYNNNNRTG 1 cut(s) 410
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 5 cut(s) 221, 230, 353, 479, 575
NdeI CATATG 1 cut(s) 580
NdeII GATC 1 cut(s) 705
NlaIII CATG 3 cut(s) 97, 250, 274
NlaIV GGNNCC 1 cut(s) 655
NmuCI GTSAC 2 cut(s) 367, 718
NspI RCATGY 1 cut(s) 97
PaeI GCATGC 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PfeI GAWTC 1 cut(s) 763
PflFI GACNNNGTC 1 cut(s) 463
PkrI GCNGC 3 cut(s) 295, 298, 349
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 655
PsyI GACNNNGTC 1 cut(s) 463
RsaI GTAC 2 cut(s) 76, 173
RsaNI GTAC 2 cut(s) 75, 172
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 585, 639
SatI GCNGC 3 cut(s) 294, 297, 348
Sau3AI GATC 1 cut(s) 705
SchI GAGTC 1 cut(s) 67
SduI GDGCHC 2 cut(s) 324, 658
SfaNI GCATC 1 cut(s) 531
SfcI CTRYAG 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SphI GCATGC 1 cut(s) 97
Sse9I AATT 2 cut(s) 150, 219
SsiI CCGC 1 cut(s) 329
SstI GAGCTC 1 cut(s) 324
StyI CCWWGG 2 cut(s) 62, 225
TaaI ACNGT 2 cut(s) 463, 703
TaiI ACGT 2 cut(s) 76, 591
TaqI TCGA 2 cut(s) 162, 645
TasI AATT 2 cut(s) 150, 219
TfiI GAWTC 1 cut(s) 763
Tru1I TTAA 2 cut(s) 585, 639
Tru9I TTAA 2 cut(s) 585, 639
TseFI GTSAC 2 cut(s) 367, 718
TseI GCWGC 3 cut(s) 293, 296, 347
Tsp45I GTSAC 2 cut(s) 367, 718
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 1 cut(s) 675
Tth111I GACNNNGTC 1 cut(s) 463
XceI RCATGY 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.