RchiOBHm_Chr2g0159471

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001481
Physical Location & Seq
Forward (+)
75575121 .. 75578727
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52804

Sequence Viewer

Length: 1527 bp
ATGGGTTCTTCCCAAGACGATACCTACCAACCTCTCCTTCAACCCATTTCATCCTCCCCATCCTTAACAGATAAGCATGACCACTCGAGTAGCACTGAGCTGGAAAGAATATTATCCGACACAGATAGGCCGTTCTTGCAGCGTCTCAAACCTGCTCTATGGATCGAGTCGAAGCTCCTTTTCTTCCTCGCCGGTCCGGCAGTGGTCGTCTACATGATCAACTACGTTATGTCCATGTCCACCCAAATCTTCTCCGGCCACCTTGGTAATCTTGAGCTCGCCGCTGCTTCTCTCGGAAACAATGGAATCCAAGTGTTCGCCTATGGCCTCATGTTAGGCATGGGAAGTGCGGTGGAGACGCTATGTGGACAAGCATATGGAGCTCAAAAGTACGAAATGCTAGGCATATATCTACAAAGATCAACCATCCTCCTAACGTTGGTCGGTTTCCTCCTCACCTTGGTCTACATCTTCTCAGAACCAGCTCTAATCTTTCTCGGCGAGTCCCCCAGAATCGCCGCTGCCGCCGCCATTTACGTCTACGGCCTGATCCCTCAAATCTTTGCCTACGCCGTCAACTTCCCGATCCAGAAATTCCTCCAAGCTCAAAGCATAGTGACTCCAAGTGCATACATATCAGCAGTGACACTAGCCGTACACCTCTTGCTGAGCTGGGTGGCCGTGTACAAGATTGGGCTTGGGCTTCTGGGCGCGTCGCTGGCGCTCAGCCTGTCGTGGTGGATCACTGTGGTGGCGCAGTTTGTATACATCGTTAAAAGCGAGAGGTGCAAGCACACGTGGAGCGGTTTTACGTGGCAAGCGTTCACCGGGTTGTATGGATTCTTCAAATTATCGGTGGCGTCTGCAGTGATGCTTTGTTTAGAGACGTGGTATTTTCAGATTCTGGTTCTGCTGGCTGGCTTGCTGGAAAATCCTGAGTTGGCTCTTGACTCGCTCTCGATTTGCACGACTATATCAGGATGGGTGTTCATGATCTCAGTTGGGTTAAATGCAGCTGCAAGTGTGCGAGTAAGCAATGAGCTTGGAGCCGGAAATCCCAAGTCCACAGCATTTTCTGTCATCGTGGTGACTACGATCTCCTTCATCATCTCCGTCATCGCAGCAACAGTTGTTCTTATTTTTCGTGATGTTATCAGCTACGCCTTCACGGAGGGTGAAGTCGTGGCCGCTGCTGTCTCAGATCTTACCCCTCTTCTTGCCCTCACCCTCCTCCTCAATGGAATTCAGCCTGTCTTGTCCGGTGTTGCTGTTGGGTGTGGATGGCAAGCTTTTGTGGCGTATGTAAATGTGGGCTGTTATTACTTTGTTGGAGTACCACTTGGTGCCCTTCTTGGATTTTACTTCAACTTTGGCGCTAAGGGAATATGGTTAGGGATGATGGCGGGCACGTTGATGCAAACAATCATTCTAATATGGATCACCGTTCAAACAGACTGGAAGAAGGAGGTGGAAGAGGCAGCCAAAAGGTTGAACAAGTGGGACGTGAAGGAACCAGTAAAAGGCTGA

Protein Analysis

508

Amino Acids

55.17

Weight (kDa)

5.79

Isoelectric Point (pI)

27.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 66 - 226 1e-34 MatE
MatE PF01554 288 - 449 9.4e-30 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 160
AccB1I GGYRCC 1 cut(s) 1343
AccBSI CCGCTC 1 cut(s) 804
AccI GTMKAC 4 cut(s) 210, 465, 540, 765
AccII CGCG 1 cut(s) 713
AciI CCGC 8 cut(s) 282, 350, 519, 525, 528, 804, 1188, 1403
AclI AACGTT 1 cut(s) 437
AclWI GGATC 5 cut(s) 170, 544, 580, 749, 1445
AcoI YGGCCR 3 cut(s) 256, 678, 1185
AcsI RAATTY 2 cut(s) 593, 1242
AcvI CACGTG 1 cut(s) 798
AcyI GRCGYC 1 cut(s) 860
AdeI CACNNNGTG 1 cut(s) 1343
AfaI GTAC 4 cut(s) 392, 657, 686, 1335
AfiI CCNNNNNNNGG 3 cut(s) 439, 460, 1520
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 5 cut(s) 41, 847, 1366, 1448, 1492
AjiI CACGTC 2 cut(s) 888, 1504
AjuI GAANNNNNNNTTGG 2 cut(s) 21, 53
AleI CACNNNNGTG 1 cut(s) 749
Alw21I GWGCWC 2 cut(s) 279, 385
Alw26I GTCTC 4 cut(s) 149, 350, 878, 1201
AlwI GGATC 5 cut(s) 170, 544, 580, 749, 1445
AlwNI CAGNNNCTG 1 cut(s) 904
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 6 cut(s) 128, 256, 325, 544, 678, 1185
ApeKI GCWGC 8 cut(s) 139, 284, 521, 1013, 1016, 1121, 1190, 1478
ApoI RAATTY 2 cut(s) 593, 1242
AspLEI GCGC 4 cut(s) 713, 724, 757, 1376
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 829
AsuHPI GGTGA 6 cut(s) 448, 817, 1099, 1187, 1216, 1432
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 194
BaeGI GKGCMC 2 cut(s) 1348, 1409
BanI GGYRCC 1 cut(s) 1343
BanII GRGCYC 2 cut(s) 279, 385
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BbrPI CACGTG 1 cut(s) 798
Bbv12I GWGCWC 2 cut(s) 279, 385
BbvI GCAGC 8 cut(s) 151, 271, 508, 1003, 1025, 1133, 1177, 1490
BccI CCATC 5 cut(s) 67, 434, 975, 1275, 1393
BceAI ACGGC 5 cut(s) 115, 557, 559, 638, 665
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 1 cut(s) 829
BcoDI GTCTC 4 cut(s) 149, 350, 878, 1201
BfaI CTAG 2 cut(s) 401, 650
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 2 cut(s) 725, 1377
BfuAI ACCTGC 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 197
BglII AGATCT 1 cut(s) 1201
BlpI GCTNAGC 2 cut(s) 668, 725
Bme1390I CCNGG 1 cut(s) 829
Bme18I GGWCC 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 85
BmgBI CACGTC 2 cut(s) 888, 1504
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 3 cut(s) 1048, 1345, 1512
BmrFI CCNGG 1 cut(s) 829
BmsI GCATC 2 cut(s) 861, 1404
Bpu10I CCTNAGC 1 cut(s) 1377
Bpu1102I GCTNAGC 2 cut(s) 668, 725
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 829
BsaAI YACGTR 2 cut(s) 798, 813
BsaHI GRCGYC 1 cut(s) 860
BsaJI CCNNGG 2 cut(s) 262, 459
BsaWI WCCGGW 1 cut(s) 1259
Bsc4I CCNNNNNNNGG 3 cut(s) 439, 460, 1520
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 1460, 1514
Bse3DI GCAATG 1 cut(s) 1042
BseDI CCNNGG 2 cut(s) 262, 459
BseGI GGATG 6 cut(s) 50, 59, 426, 986, 1286, 1401
BseLI CCNNNNNNNGG 3 cut(s) 439, 460, 1520
BseMI GCAATG 1 cut(s) 1042
BseMII CTCAG 7 cut(s) 87, 489, 659, 739, 927, 1011, 1212
BseNI ACTGG 2 cut(s) 1460, 1514
BseRI GAGGAG 3 cut(s) 443, 1220, 1223
BseSI GKGCMC 2 cut(s) 1348, 1409
BseXI GCAGC 8 cut(s) 151, 271, 508, 1003, 1025, 1133, 1177, 1490
BseYI CCCAGC 1 cut(s) 672
Bsh1236I CGCG 1 cut(s) 713
BshFI GGCC 6 cut(s) 130, 258, 327, 546, 680, 1187
BshNI GGYRCC 1 cut(s) 1343
BsiHKAI GWGCWC 2 cut(s) 279, 385
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 6 cut(s) 192, 197, 255, 828, 1050, 1260
BslFI GGGAC 2 cut(s) 490, 1514
BslI CCNNNNNNNGG 3 cut(s) 439, 460, 1520
BsmAI GTCTC 4 cut(s) 149, 350, 878, 1201
BsmBI CGTCTC 3 cut(s) 149, 350, 878
BsmFI GGGAC 2 cut(s) 490, 1514
BsnI GGCC 6 cut(s) 130, 258, 327, 546, 680, 1187
BsoBI CYCGRG 1 cut(s) 85
Bsp1286I GDGCHC 4 cut(s) 279, 385, 1348, 1409
Bsp1407I TGTACA 1 cut(s) 684
Bsp1720I GCTNAGC 2 cut(s) 668, 725
BspACI CCGC 8 cut(s) 282, 350, 519, 525, 528, 804, 1188, 1403
BspANI GGCC 6 cut(s) 130, 258, 327, 546, 680, 1187
BspCNI CTCAG 7 cut(s) 88, 488, 660, 738, 928, 1010, 1211
BspFNI CGCG 1 cut(s) 713
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 3 cut(s) 1048, 1345, 1512
BspMAI CTGCAG 1 cut(s) 868
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 5 cut(s) 170, 544, 580, 749, 1445
BspT107I GGYRCC 1 cut(s) 1343
BsrBI CCGCTC 1 cut(s) 804
BsrDI GCAATG 1 cut(s) 1042
BsrFI RCCGGY 1 cut(s) 191
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 2 cut(s) 1460, 1514
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 262, 459
BssNAI GTATAC 1 cut(s) 766
BssNI GRCGYC 1 cut(s) 860
BssT1I CCWWGG 2 cut(s) 262, 459
Bst1107I GTATAC 1 cut(s) 766
Bst4CI ACNGT 3 cut(s) 748, 1129, 1444
Bst6I CTCTTC 2 cut(s) 1218, 1467
BstACI GRCGYC 1 cut(s) 860
BstAUI TGTACA 1 cut(s) 684
BstBAI YACGTR 2 cut(s) 798, 813
BstC8I GCNNGC 9 cut(s) 279, 720, 791, 819, 915, 919, 923, 1287, 1405
BstDEI CTNAG 8 cut(s) 96, 475, 668, 725, 936, 997, 1198, 1377
BstF5I GGATG 6 cut(s) 50, 59, 426, 986, 1286, 1401
BstFNI CGCG 1 cut(s) 713
BstH2I RGCGCY 2 cut(s) 725, 1377
BstHHI GCGC 4 cut(s) 713, 724, 757, 1376
BstMAI GTCTC 4 cut(s) 149, 350, 878, 1201
BstMWI GCNNNNNNNGC 8 cut(s) 136, 197, 380, 524, 527, 719, 786, 1295
BstSCI CCNGG 1 cut(s) 827
BstSFI CTRYAG 1 cut(s) 864
BstSLI GKGCMC 2 cut(s) 1348, 1409
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 8 cut(s) 151, 271, 508, 1003, 1025, 1133, 1177, 1490
BstX2I RGATCY 1 cut(s) 1201
BstYI RGATCY 1 cut(s) 1201
BstZ17I GTATAC 1 cut(s) 766
BsuRI GGCC 6 cut(s) 130, 258, 327, 546, 680, 1187
BtgZI GCGATG 1 cut(s) 1102
BtrI CACGTC 2 cut(s) 888, 1504
BtsCI GGATG 6 cut(s) 50, 59, 426, 986, 1286, 1401
BtsI GCAGTG 3 cut(s) 207, 648, 873
BtsIMutI CAGTG 5 cut(s) 93, 207, 648, 744, 873
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 9 cut(s) 279, 720, 791, 819, 915, 919, 923, 1287, 1405
CaiI CAGNNNCTG 1 cut(s) 904
CciI TCATGA 1 cut(s) 990
CfoI GCGC 4 cut(s) 713, 724, 757, 1376
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 194
CpoI CGGWCCG 1 cut(s) 194
CseI GACGC 4 cut(s) 131, 367, 702, 849
Csp6I GTAC 4 cut(s) 391, 656, 685, 1334
CspI CGGWCCG 1 cut(s) 194
CviAII CATG 6 cut(s) 77, 214, 235, 331, 340, 991
CviQI GTAC 4 cut(s) 391, 656, 685, 1334
DdeI CTNAG 8 cut(s) 96, 475, 668, 725, 936, 997, 1198, 1377
DraIII CACNNNGTG 1 cut(s) 1343
EaeI YGGCCR 3 cut(s) 256, 678, 1185
Eam1104I CTCTTC 2 cut(s) 1218, 1467
EarI CTCTTC 2 cut(s) 1218, 1467
Ecl136II GAGCTC 2 cut(s) 277, 383
Eco130I CCWWGG 2 cut(s) 262, 459
Eco24I GRGCYC 2 cut(s) 279, 385
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 2 cut(s) 277, 383
Eco72I CACGTG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 85
EcoICRI GAGCTC 2 cut(s) 277, 383
EcoRI GAATTC 1 cut(s) 1242
EcoT14I CCWWGG 2 cut(s) 262, 459
EcoT38I GRGCYC 2 cut(s) 279, 385
ErhI CCWWGG 2 cut(s) 262, 459
Esp3I CGTCTC 3 cut(s) 149, 350, 878
FaeI CATG 6 cut(s) 80, 217, 238, 334, 343, 994
FaqI GGGAC 2 cut(s) 490, 1514
FatI CATG 6 cut(s) 76, 213, 234, 330, 339, 990
FauI CCCGC 1 cut(s) 1396
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 4 cut(s) 210, 465, 540, 765
FokI GGATG 6 cut(s) 37, 46, 413, 993, 1293, 1408
FriOI GRGCYC 2 cut(s) 279, 385
FspBI CTAG 2 cut(s) 401, 650
GlaI GCGC 4 cut(s) 712, 723, 756, 1375
GsaI CCCAGC 1 cut(s) 676
HaeII RGCGCY 2 cut(s) 725, 1377
HaeIII GGCC 6 cut(s) 130, 258, 327, 546, 680, 1187
HapII CCGG 6 cut(s) 192, 197, 255, 828, 1050, 1260
HgaI GACGC 4 cut(s) 131, 367, 702, 849
HhaI GCGC 4 cut(s) 713, 724, 757, 1376
Hin1I GRCGYC 1 cut(s) 860
Hin1II CATG 6 cut(s) 80, 217, 238, 334, 343, 994
Hin6I GCGC 4 cut(s) 711, 722, 755, 1374
HinP1I GCGC 4 cut(s) 711, 722, 755, 1374
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 1287
HinfI GANTC 8 cut(s) 167, 306, 503, 513, 619, 840, 901, 950
HpaII CCGG 6 cut(s) 192, 197, 255, 828, 1050, 1260
HphI GGTGA 6 cut(s) 448, 817, 1099, 1187, 1216, 1432
Hpy188I TCNGA 5 cut(s) 118, 296, 478, 900, 1201
Hpy188III TCNNGA 9 cut(s) 272, 583, 589, 935, 947, 958, 978, 991, 1145
Hpy99I CGWCG 1 cut(s) 718
HpyAV CCTTC 6 cut(s) 47, 1111, 1174, 1358, 1456, 1501
HpyCH4III ACNGT 3 cut(s) 748, 1129, 1444
HpyCH4IV ACGT 8 cut(s) 225, 437, 537, 797, 812, 887, 1409, 1503
HpyCH4V TGCA 8 cut(s) 139, 629, 789, 866, 966, 1013, 1019, 1417
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 197, 380, 524, 527, 719, 786, 1295
HpyF3I CTNAG 8 cut(s) 96, 475, 668, 725, 936, 997, 1198, 1377
HpySE526I ACGT 8 cut(s) 225, 437, 537, 797, 812, 887, 1409, 1503
Hsp92I GRCGYC 1 cut(s) 860
Hsp92II CATG 6 cut(s) 80, 217, 238, 334, 343, 994
HspAI GCGC 4 cut(s) 711, 722, 755, 1374
Ksp22I TGATCA 1 cut(s) 216
LmnI GCTCC 4 cut(s) 180, 380, 801, 1046
Lsp1109I GCAGC 8 cut(s) 151, 271, 508, 1003, 1025, 1133, 1177, 1490
LweI GCATC 2 cut(s) 861, 1404
MaeI CTAG 2 cut(s) 401, 650
MaeII ACGT 8 cut(s) 225, 437, 537, 797, 812, 887, 1409, 1503
MaeIII GTNAC 3 cut(s) 616, 643, 1087
MbiI CCGCTC 1 cut(s) 804
MboII GAAGA 7 cut(s) 175, 241, 463, 835, 1205, 1471, 1484
MflI RGATCY 1 cut(s) 1201
MhlI GDGCHC 4 cut(s) 279, 385, 1348, 1409
MluCI AATT 3 cut(s) 593, 848, 1242
MlyI GAGTC 4 cut(s) 176, 512, 613, 944
MmeI TCCRAC 2 cut(s) 141, 1309
MseI TTAA 3 cut(s) 65, 774, 1007
MslI CAYNNNNRTG 3 cut(s) 749, 1085, 1412
MspA1I CMGCKG 4 cut(s) 284, 521, 1016, 1190
MspI CCGG 6 cut(s) 192, 197, 255, 828, 1050, 1260
MspR9I CCNGG 1 cut(s) 829
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 8 cut(s) 136, 197, 380, 524, 527, 719, 786, 1295
NciI CCSGG 1 cut(s) 829
NdeI CATATG 1 cut(s) 376
NlaIII CATG 6 cut(s) 80, 217, 238, 334, 343, 994
NlaIV GGNNCC 3 cut(s) 1048, 1345, 1512
NmeAIII GCCGAG 1 cut(s) 477
NmuCI GTSAC 3 cut(s) 616, 643, 1087
OliI CACNNNNGTG 1 cut(s) 749
PaeR7I CTCGAG 1 cut(s) 85
PagI TCATGA 1 cut(s) 990
PcsI WCGNNNNNNNCGW 3 cut(s) 777, 818, 965
PfeI GAWTC 4 cut(s) 306, 513, 840, 901
PleI GAGTC 4 cut(s) 175, 511, 613, 944
PmaCI CACGTG 1 cut(s) 798
PmlI CACGTG 1 cut(s) 798
PpsI GAGTC 4 cut(s) 175, 511, 613, 944
Ppu21I YACGTR 2 cut(s) 798, 813
Psp124BI GAGCTC 2 cut(s) 279, 385
Psp1406I AACGTT 1 cut(s) 437
PspCI CACGTG 1 cut(s) 798
PspFI CCCAGC 1 cut(s) 672
PspN4I GGNNCC 3 cut(s) 1048, 1345, 1512
PspPI GGNCC 1 cut(s) 194
PspXI VCTCGAGB 1 cut(s) 85
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 1 cut(s) 904
PsuI RGATCY 1 cut(s) 1201
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 4 cut(s) 392, 657, 686, 1335
RsaNI GTAC 4 cut(s) 391, 656, 685, 1334
RseI CAYNNNNRTG 3 cut(s) 749, 1085, 1412
Rsr2I CGGWCCG 1 cut(s) 194
RsrII CGGWCCG 1 cut(s) 194
SacI GAGCTC 2 cut(s) 279, 385
SaqAI TTAA 3 cut(s) 65, 774, 1007
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 4 cut(s) 176, 512, 613, 944
ScrFI CCNGG 1 cut(s) 829
SduI GDGCHC 4 cut(s) 279, 385, 1348, 1409
SfaNI GCATC 2 cut(s) 861, 1404
SfcI CTRYAG 1 cut(s) 864
Sfr274I CTCGAG 1 cut(s) 85
SinI GGWCC 1 cut(s) 194
SlaI CTCGAG 1 cut(s) 85
SmiMI CAYNNNNRTG 3 cut(s) 749, 1085, 1412
SmlI CTYRAG 2 cut(s) 85, 272
SmoI CTYRAG 2 cut(s) 85, 272
Sse9I AATT 3 cut(s) 593, 848, 1242
SsiI CCGC 8 cut(s) 282, 350, 519, 525, 528, 804, 1188, 1403
SspI AATATT 1 cut(s) 111
SspMI CTAG 2 cut(s) 401, 650
SstI GAGCTC 2 cut(s) 279, 385
StyD4I CCNGG 1 cut(s) 827
StyI CCWWGG 2 cut(s) 262, 459
TaaI ACNGT 3 cut(s) 748, 1129, 1444
TaiI ACGT 8 cut(s) 228, 440, 540, 800, 815, 890, 1412, 1506
TaqI TCGA 4 cut(s) 86, 165, 170, 959
TasI AATT 3 cut(s) 593, 848, 1242
TatI WGTACW 1 cut(s) 684
TauI GCSGC 5 cut(s) 284, 521, 527, 530, 1190
TfiI GAWTC 4 cut(s) 306, 513, 840, 901
Tru1I TTAA 3 cut(s) 65, 774, 1007
Tru9I TTAA 3 cut(s) 65, 774, 1007
TscAI CASTG 5 cut(s) 100, 207, 648, 751, 873
TseFI GTSAC 3 cut(s) 616, 643, 1087
TseI GCWGC 8 cut(s) 139, 284, 521, 1013, 1016, 1121, 1190, 1478
Tsp45I GTSAC 3 cut(s) 616, 643, 1087
TspDTI ATGAA 3 cut(s) 39, 979, 1093
TspGWI ACGGA 2 cut(s) 1102, 1184
TspRI CASTG 5 cut(s) 100, 207, 648, 751, 873
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 2 cut(s) 593, 1242
XhoI CTCGAG 1 cut(s) 85
XmiI GTMKAC 4 cut(s) 210, 465, 540, 765
XspI CTAG 2 cut(s) 401, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.