Rw5G002680

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001481
Physical Location & Seq
Forward (+)
2542501 .. 2546676
4176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002680.1

Sequence Viewer

Length: 1449 bp
ATGGACTACTCCAGTGATAGTAATGAGCTCTCTGAACCCATCTTGACTGACAAAAGATGTTCATCTGTTCAACTAGTGAGCTCGGAACTGGAAGAAACGTTATCCAACACCGAGCTTCCGTACTTCCACCGTCTTAGACGTGCCACGTGGGTCGAACTGAAGATTCTCTTCCGCCTTGCAGCTCCGGCAGTGGTGGTTTACTTGCTAAACAACGTTATTTCTATGTCCACCCAAATCTACTGCGGGCATCTCGGCAATCTCGAACTCGCTGCCTCTTCTCTTGGCAACACCGGCATCCAAGTTTTTGCCTACGGCCTCATGCTTGGAATGGGAAGTGCGGTGGAAACGCTCTGTGGTCAAGCCTATGGAGCAAAGAAATATGACATGCTAGGCGTATATCTGCAGCGTTCAACGATCCTGCTAATGGCAACTGGGATCCCAGTTATGTTCATCTACATCTTCTCCGAGTCCATCTTGATTGCCCTAGGCGAATCGGCCACCATTGCCTCTGCAGCCGCAATCTTCGTTTACGGACTTATTCCCCAAATCTTTGCCTACGCTTGCAATTTTCCCATACAAAAATTCCTCCAGGCTCAGAGCATCGTGTTCCCGAGCGCGTACATATCCGCGGCTGCCCTCGTGGTGCATGTGGTGCTGAGTTGGGTAGTGATTTATAAGTTGGGATGGGGCTTGTTAGGCGCATCGCTGATGTTGAGCTTTTCGTGGTGGATTATAGTGGTGGCGCAGTTTGTTTACATATTGCTGAGTCCAAGGTGTAAACGTACTTGGACAGGTTTTAGCATGCAGGCTTTTTCTGGGCTTTGGACTTTTCTGAAGCTATCTGTTGCATCTGCCGTAATGCTTTGCCTCGAGACTTGGTACTATCAGATTATCGTTTTGATTGCTGGGTTGCTTGAAGACGCTGAAATTGCCTTGGACGCTCTCTCTGTTTGCATGACCATATCTGGATGGGTGTTCATGGTTGCTGTGGGTTTCAATGCTGCTGCAAGTGTGAGGATTGGGAATGAGCTCGGGGCGGGACATCCAAAGTCGGCCGCATTTGCAGTAGTGTGTGCCATAGTTGTGCTTGCACTACGAAATGTCATAAGCTACGTGTCCAATGCAGTCTCACTTACCTTGCTGTCTCTATTATGCTCAACGGCATCCAACCTGTTTTGGGTGGCTGTTGGGTGTGGATGGCAAGCATTTGTGGCATATGTTAACGTCGGATGTTACTACATTATTGGTATTCCTTTGGGTTGTGTTCTTGGCTTCAAGTTTGACTTTGGAGCCCAGGGAATATGGTCTGGAATGATAGGAGGGACATTTATACAGACGGCGATCTTATTGTGGGTCACACTTCGGACTGATTGGAATAAAGAGGTCGCAAATGCAAAGAATCGTTTGGACAAGTGGGATGACAAGAAAGAGCCTCTTCTCACAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

482

Amino Acids

52.65

Weight (kDa)

6.13

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 62 - 222 1.4e-34 MatE
MatE PF01554 284 - 384 5.4e-14 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AccII CGCG 2 cut(s) 617, 629
AciI CCGC 8 cut(s) 172, 243, 338, 516, 627, 629, 1037, 1056
AclI AACGTT 2 cut(s) 98, 213
AclWI GGATC 3 cut(s) 409, 430, 443
AcoI YGGCCR 2 cut(s) 495, 1053
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 179, 854
AcvI CACGTG 1 cut(s) 147
AfaI GTAC 4 cut(s) 122, 620, 784, 881
AfiI CCNNNNNNNGG 2 cut(s) 424, 1177
AflIII ACRYGT 1 cut(s) 1113
AgsI TTSAA 5 cut(s) 71, 411, 917, 997, 1276
AhlI ACTAGT 1 cut(s) 73
AjiI CACGTC 1 cut(s) 140
AjnI CCWGG 2 cut(s) 588, 1293
AluBI AGCT 8 cut(s) 28, 81, 115, 182, 717, 838, 1030, 1110
AluI AGCT 8 cut(s) 28, 81, 115, 182, 717, 838, 1030, 1110
Alw21I GWGCWC 3 cut(s) 30, 83, 1032
Alw26I GTCTC 3 cut(s) 866, 1132, 1149
AlwI GGATC 3 cut(s) 409, 430, 443
Ama87I CYCGRG 3 cut(s) 610, 869, 1031
AoxI GGCC 3 cut(s) 313, 495, 1053
ApeKI GCWGC 7 cut(s) 179, 269, 403, 512, 632, 1001, 1004
ApoI RAATTY 1 cut(s) 581
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 3 cut(s) 617, 701, 745
AvaI CYCGRG 3 cut(s) 610, 869, 1031
AvrII CCTAGG 1 cut(s) 484
BamHI GGATCC 1 cut(s) 435
BanII GRGCYC 4 cut(s) 30, 83, 1032, 1294
BauI CACGAG 1 cut(s) 638
BbrPI CACGTG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 3 cut(s) 30, 83, 1032
BbvI GCAGC 7 cut(s) 191, 256, 415, 524, 619, 988, 991
BccI CCATC 5 cut(s) 47, 479, 678, 963, 1191
BceAI ACGGC 4 cut(s) 328, 839, 1176, 1353
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 590, 1295
BcoDI GTCTC 3 cut(s) 866, 1132, 1149
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 3 cut(s) 74, 389, 485
BfmI CTRYAG 2 cut(s) 401, 510
BlnI CCTAGG 1 cut(s) 484
Bme1390I CCNGG 2 cut(s) 590, 1295
BmeT110I CYCGRG 3 cut(s) 610, 869, 1031
BmgBI CACGTC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 437, 1291
BmrFI CCNGG 2 cut(s) 590, 1295
BmrI ACTGGG 2 cut(s) 434, 441
BmsI GCATC 6 cut(s) 256, 303, 609, 710, 857, 1172
BmuI ACTGGG 2 cut(s) 434, 441
BpiI GAAGAC 1 cut(s) 924
BpmI CTGGAG 1 cut(s) 572
BsaAI YACGTR 2 cut(s) 147, 1114
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 6 cut(s) 484, 627, 770, 933, 1293, 1294
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 1177
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 4 cut(s) 12, 93, 436, 440
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 1 cut(s) 61
BseBI CCWGG 2 cut(s) 590, 1295
BseDI CCNNGG 6 cut(s) 484, 627, 770, 933, 1293, 1294
BseGI GGATG 8 cut(s) 294, 689, 974, 1042, 1163, 1202, 1235, 1423
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 424, 1177
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 608, 647, 755
BseNI ACTGG 4 cut(s) 12, 93, 436, 440
BseX3I CGGCCG 1 cut(s) 1053
BseXI GCAGC 7 cut(s) 191, 256, 415, 524, 619, 988, 991
BseYI CCCAGC 1 cut(s) 905
Bsh1236I CGCG 2 cut(s) 617, 629
Bsh1285I CGRYCG 1 cut(s) 1056
BshFI GGCC 3 cut(s) 315, 497, 1055
BsiEI CGRYCG 1 cut(s) 1056
BsiHKAI GWGCWC 3 cut(s) 30, 83, 1032
BsiHKCI CYCGRG 3 cut(s) 610, 869, 1031
BsiSI CCGG 2 cut(s) 185, 291
BslFI GGGAC 2 cut(s) 1053, 1336
BslI CCNNNNNNNGG 2 cut(s) 424, 1177
BsmAI GTCTC 3 cut(s) 866, 1132, 1149
BsmFI GGGAC 2 cut(s) 1053, 1336
BsnI GGCC 3 cut(s) 315, 497, 1055
BsoBI CYCGRG 3 cut(s) 610, 869, 1031
Bsp1286I GDGCHC 4 cut(s) 30, 83, 1032, 1294
Bsp143I GATC 3 cut(s) 414, 435, 1341
BspACI CCGC 8 cut(s) 172, 243, 338, 516, 627, 629, 1037, 1056
BspANI GGCC 3 cut(s) 315, 497, 1055
BspCNI CTCAG 3 cut(s) 607, 648, 756
BspFNI CGCG 2 cut(s) 617, 629
BspLI GGNNCC 2 cut(s) 437, 1291
BspMAI CTGCAG 2 cut(s) 405, 514
BspPI GGATC 3 cut(s) 409, 430, 443
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 4 cut(s) 12, 93, 436, 440
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 6 cut(s) 484, 627, 770, 933, 1293, 1294
BssMI GATC 3 cut(s) 414, 435, 1341
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 3 cut(s) 484, 770, 933
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 2 cut(s) 590, 1295
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 3 cut(s) 173, 280, 1440
BstAPI GCANNNNNTGC 1 cut(s) 652
BstBAI YACGTR 2 cut(s) 147, 1114
BstC8I GCNNGC 6 cut(s) 245, 562, 803, 807, 1089, 1203
BstDEI CTNAG 4 cut(s) 134, 594, 656, 764
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 8 cut(s) 294, 689, 974, 1042, 1163, 1202, 1235, 1423
BstFNI CGCG 2 cut(s) 617, 629
BstHHI GCGC 3 cut(s) 617, 701, 745
BstKTI GATC 3 cut(s) 417, 438, 1344
BstMAI GTCTC 3 cut(s) 866, 1132, 1149
BstMBI GATC 3 cut(s) 414, 435, 1341
BstMCI CGRYCG 1 cut(s) 1056
BstNI CCWGG 2 cut(s) 590, 1295
BstNSI RCATGY 3 cut(s) 388, 650, 805
BstSCI CCNGG 2 cut(s) 588, 1293
BstSFI CTRYAG 2 cut(s) 401, 510
BstUI CGCG 2 cut(s) 617, 629
BstV1I GCAGC 7 cut(s) 191, 256, 415, 524, 619, 988, 991
BstV2I GAAGAC 1 cut(s) 924
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BstZI CGGCCG 1 cut(s) 1053
BsuRI GGCC 3 cut(s) 315, 497, 1055
BtgI CCRYGG 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 8 cut(s) 294, 689, 974, 1042, 1163, 1202, 1235, 1423
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 19, 195
Cac8I GCNNGC 6 cut(s) 245, 562, 803, 807, 1089, 1203
CfoI GCGC 3 cut(s) 617, 701, 745
Cfr10I RCCGGY 1 cut(s) 290
Cfr42I CCGCGG 1 cut(s) 630
CseI GACGC 2 cut(s) 929, 947
Csp6I GTAC 4 cut(s) 121, 619, 783, 880
CviAII CATG 6 cut(s) 319, 385, 647, 802, 955, 979
CviQI GTAC 4 cut(s) 121, 619, 783, 880
DdeI CTNAG 4 cut(s) 134, 594, 656, 764
DpnI GATC 3 cut(s) 416, 437, 1343
DpnII GATC 3 cut(s) 414, 435, 1341
EaeI YGGCCR 2 cut(s) 495, 1053
EagI CGGCCG 1 cut(s) 1053
Eam1104I CTCTTC 3 cut(s) 173, 280, 1440
EarI CTCTTC 3 cut(s) 173, 280, 1440
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 3 cut(s) 28, 81, 1030
EclXI CGGCCG 1 cut(s) 1053
Eco130I CCWWGG 3 cut(s) 484, 770, 933
Eco24I GRGCYC 4 cut(s) 30, 83, 1032, 1294
Eco52I CGGCCG 1 cut(s) 1053
Eco53kI GAGCTC 3 cut(s) 28, 81, 1030
Eco57I CTGAAG 2 cut(s) 179, 854
Eco72I CACGTG 1 cut(s) 147
Eco88I CYCGRG 3 cut(s) 610, 869, 1031
EcoICRI GAGCTC 3 cut(s) 28, 81, 1030
EcoRII CCWGG 2 cut(s) 588, 1293
EcoT14I CCWWGG 3 cut(s) 484, 770, 933
EcoT38I GRGCYC 4 cut(s) 30, 83, 1032, 1294
ErhI CCWWGG 3 cut(s) 484, 770, 933
FaeI CATG 6 cut(s) 322, 388, 650, 805, 958, 982
FalI AAGNNNNNCTT 5 cut(s) 152, 184, 1268, 1300, 1419
FaqI GGGAC 2 cut(s) 1053, 1336
FatI CATG 6 cut(s) 318, 384, 646, 801, 954, 978
FauI CCCGC 2 cut(s) 236, 1030
FauNDI CATATG 1 cut(s) 1216
FokI GGATG 8 cut(s) 281, 696, 981, 1029, 1150, 1209, 1242, 1430
FriOI GRGCYC 4 cut(s) 30, 83, 1032, 1294
FspBI CTAG 3 cut(s) 74, 389, 485
GlaI GCGC 3 cut(s) 616, 700, 744
GsaI CCCAGC 1 cut(s) 909
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 3 cut(s) 315, 497, 1055
HapII CCGG 2 cut(s) 185, 291
HgaI GACGC 2 cut(s) 929, 947
HhaI GCGC 3 cut(s) 617, 701, 745
Hin1II CATG 6 cut(s) 322, 388, 650, 805, 958, 982
Hin6I GCGC 3 cut(s) 615, 699, 743
HinP1I GCGC 3 cut(s) 615, 699, 743
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HinfI GANTC 5 cut(s) 163, 467, 491, 766, 1399
HpaI GTTAAC 1 cut(s) 1222
HpaII CCGG 2 cut(s) 185, 291
Hpy166II GTNNAC 6 cut(s) 199, 228, 529, 754, 779, 1222
Hpy188I TCNGA 8 cut(s) 34, 85, 466, 597, 834, 888, 1229, 1365
Hpy188III TCNNGA 7 cut(s) 43, 260, 475, 610, 871, 966, 1308
Hpy8I GTNNAC 6 cut(s) 199, 228, 529, 754, 779, 1222
Hpy99I CGWCG 1 cut(s) 1229
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 7 cut(s) 98, 139, 146, 213, 781, 1113, 1224
HpyF3I CTNAG 4 cut(s) 134, 594, 656, 764
HpySE526I ACGT 7 cut(s) 98, 139, 146, 213, 781, 1113, 1224
Hsp92II CATG 6 cut(s) 322, 388, 650, 805, 958, 982
HspAI GCGC 3 cut(s) 615, 699, 743
KspAI GTTAAC 1 cut(s) 1222
KspI CCGCGG 1 cut(s) 630
Kzo9I GATC 3 cut(s) 414, 435, 1341
LmnI GCTCC 3 cut(s) 187, 368, 1289
Lsp1109I GCAGC 7 cut(s) 191, 256, 415, 524, 619, 988, 991
LweI GCATC 6 cut(s) 256, 303, 609, 710, 857, 1172
MaeI CTAG 3 cut(s) 74, 389, 485
MaeII ACGT 7 cut(s) 98, 139, 146, 213, 781, 1113, 1224
MaeIII GTNAC 2 cut(s) 1232, 1354
MalI GATC 3 cut(s) 416, 437, 1343
MboI GATC 3 cut(s) 414, 435, 1341
MboII GAAGA 8 cut(s) 104, 160, 172, 267, 451, 514, 929, 1427
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 4 cut(s) 30, 83, 1032, 1294
MluCI AATT 3 cut(s) 565, 581, 927
MlyI GAGTC 2 cut(s) 476, 775
MmeI TCCRAC 3 cut(s) 129, 1191, 1207
MseI TTAA 1 cut(s) 1221
MslI CAYNNNNRTG 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 2 cut(s) 185, 291
MspR9I CCNGG 2 cut(s) 590, 1295
MvaI CCWGG 2 cut(s) 590, 1295
MvnI CGCG 2 cut(s) 617, 629
NdeI CATATG 1 cut(s) 1216
NdeII GATC 3 cut(s) 414, 435, 1341
NlaIII CATG 6 cut(s) 322, 388, 650, 805, 958, 982
NlaIV GGNNCC 2 cut(s) 437, 1291
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 1354
NspI RCATGY 3 cut(s) 388, 650, 805
PaeI GCATGC 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 869
PasI CCCWGGG 1 cut(s) 1294
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 3 cut(s) 163, 491, 1399
PleI GAGTC 2 cut(s) 475, 774
PmaCI CACGTG 1 cut(s) 147
PmlI CACGTG 1 cut(s) 147
PpsI GAGTC 2 cut(s) 475, 774
Ppu21I YACGTR 2 cut(s) 147, 1114
PsiI TTATAA 1 cut(s) 675
Psp124BI GAGCTC 3 cut(s) 30, 83, 1032
Psp1406I AACGTT 2 cut(s) 98, 213
Psp6I CCWGG 2 cut(s) 588, 1293
PspCI CACGTG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 905
PspGI CCWGG 2 cut(s) 588, 1293
PspN4I GGNNCC 2 cut(s) 437, 1291
PstI CTGCAG 2 cut(s) 405, 514
PsuI RGATCY 1 cut(s) 435
RsaI GTAC 4 cut(s) 122, 620, 784, 881
RsaNI GTAC 4 cut(s) 121, 619, 783, 880
RseI CAYNNNNRTG 1 cut(s) 1082
SacI GAGCTC 3 cut(s) 30, 83, 1032
SacII CCGCGG 1 cut(s) 630
SaqAI TTAA 1 cut(s) 1221
Sau3AI GATC 3 cut(s) 414, 435, 1341
SchI GAGTC 2 cut(s) 476, 775
ScrFI CCNGG 2 cut(s) 590, 1295
SduI GDGCHC 4 cut(s) 30, 83, 1032, 1294
SfaNI GCATC 6 cut(s) 256, 303, 609, 710, 857, 1172
SfcI CTRYAG 2 cut(s) 401, 510
Sfr274I CTCGAG 1 cut(s) 869
Sfr303I CCGCGG 1 cut(s) 630
SgrBI CCGCGG 1 cut(s) 630
SlaI CTCGAG 1 cut(s) 869
SmiMI CAYNNNNRTG 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
SpeI ACTAGT 1 cut(s) 73
SphI GCATGC 1 cut(s) 805
Sse9I AATT 3 cut(s) 565, 581, 927
SsiI CCGC 8 cut(s) 172, 243, 338, 516, 627, 629, 1037, 1056
SspMI CTAG 3 cut(s) 74, 389, 485
SstI GAGCTC 3 cut(s) 30, 83, 1032
StyD4I CCNGG 2 cut(s) 588, 1293
StyI CCWWGG 3 cut(s) 484, 770, 933
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 7 cut(s) 101, 142, 149, 216, 784, 1116, 1227
TaqI TCGA 3 cut(s) 153, 261, 870
TasI AATT 3 cut(s) 565, 581, 927
TauI GCSGC 3 cut(s) 518, 632, 1058
TfiI GAWTC 3 cut(s) 163, 491, 1399
Tru1I TTAA 1 cut(s) 1221
Tru9I TTAA 1 cut(s) 1221
TscAI CASTG 2 cut(s) 19, 195
TseFI GTSAC 1 cut(s) 1354
TseI GCWGC 7 cut(s) 179, 269, 403, 512, 632, 1001, 1004
Tsp45I GTSAC 1 cut(s) 1354
TspDTI ATGAA 3 cut(s) 51, 439, 967
TspGWI ACGGA 2 cut(s) 108, 546
TspRI CASTG 2 cut(s) 19, 195
XapI RAATTY 1 cut(s) 581
XceI RCATGY 3 cut(s) 388, 650, 805
XhoI CTCGAG 1 cut(s) 869
XmaJI CCTAGG 1 cut(s) 484
XspI CTAG 3 cut(s) 74, 389, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.