Rorug04G0402800

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001481
Physical Location & Seq
Reverse (-)
56730578 .. 56733178
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0402800.1

Sequence Viewer

Length: 999 bp
ATGGAGGCTCACGGGTCGGGGCTCCGGCGTGTCATCGTCTTGGCCTTCTGCGTCGCCGGTATCTGGGCCGCTTATATCTACCAAGGTGTTCTTCAGGAAACTCTGTCCACCAAGCGGTTCGGTCCGGATGGGATTAGGTTCGAGCATCTTTCGTTCCTGAACTTGGCCCAAAATGTGGTCTGTTTAATCTGGTCATATACAATGTTGAAGATTTGGTCCAGCAGTAATGCTGGCGGTGCGCCTTTGTGGACGTACTGGAGTGCAGGGATTACTAATACGATTGGACCGGCTATGGGAATCGAAGCTTTGAAGTATATCAGCTACCCGGCCCAGGTCCTGGCCAAATCCTCAAAAATGATTCCAGTGATGCTGATGGGTACTCTAGTCTATGGCATAAGATACACCTTTCCTGAGTATGTTTGTACTCTCCTTGTTGCTGGAGGGGTATCCATGTTTGCACTCTTGAAGACTAGCTCAAAGACCATTAGCAAGTTGGCACACCCAAATGCACCTCTTGGATATGGACTATGCTTCCTTAACCTTGCTTTTGATGGATTCACAAATGCTACTCAGGATTCAATAACAGCAAGGTACCCAAAAACAAGTGCCTGGGAAATAATGTTAGGCATGAACCTATGGGGTACCATATACAACATGATCTACATGTTTGGCTGGCCGCGAGGTAGCGGATTTGAGGCAGTTCAATTCTGCAAGATGCATCCTGAAGCAGCATGGGACATTTTTCTCTATTGTCTCTGTGGCGCAGTTGGCCAGAATTTCATCTTCCTAACCATAAGCAGATTTGGTTCCCTGGCTAATACCACCATTACCACCACCCGCAAGTTTGTGAGCATTGTGGTGTCGTCATTGCTCAGTGGCAATCCCCTGTCATCAAAGCAATGGGGTTGTGTTGTCACGGTCTTCTCTGGGTTGTCGTATCAGATCTACCTCAAATGGAAGAAGTTGCAGAGACTGCAGAAGAAGAGAAAGCCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

332

Amino Acids

36.87

Weight (kDa)

9.59

Isoelectric Point (pI)

26.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UAA PF08449 15 - 317 1.7e-77 UAA transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 591, 641
AccB1I GGYRCC 2 cut(s) 591, 641
AccB7I CCANNNNNTGG 2 cut(s) 175, 337
AccII CGCG 1 cut(s) 679
AccIII TCCGGA 1 cut(s) 124
AciI CCGC 6 cut(s) 69, 115, 234, 677, 687, 838
AcoI YGGCCR 3 cut(s) 339, 674, 769
AcsI RAATTY 1 cut(s) 775
AcuI CTGAAG 2 cut(s) 77, 744
AfaI GTAC 5 cut(s) 254, 379, 424, 593, 643
AfiI CCNNNNNNNGG 7 cut(s) 63, 114, 163, 175, 293, 331, 337
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 5 cut(s) 208, 310, 466, 579, 704
AjnI CCWGG 4 cut(s) 330, 336, 608, 810
AjuI GAANNNNNNNTTGG 2 cut(s) 75, 107
AluBI AGCT 3 cut(s) 305, 321, 474
AluI AGCT 3 cut(s) 305, 321, 474
Alw26I GTCTC 2 cut(s) 758, 964
AlwNI CAGNNNCTG 2 cut(s) 337, 973
Aor13HI TCCGGA 1 cut(s) 124
AoxI GGCC 7 cut(s) 42, 66, 165, 327, 339, 674, 769
ApeKI GCWGC 1 cut(s) 728
ApoI RAATTY 1 cut(s) 775
Asp718I GGTACC 2 cut(s) 591, 641
AspLEI GCGC 2 cut(s) 241, 764
AspS9I GGNCC 7 cut(s) 66, 122, 166, 216, 284, 328, 334
AsuC2I CCSGG 1 cut(s) 326
AvaII GGWCC 4 cut(s) 122, 216, 284, 334
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BalI TGGCCA 2 cut(s) 341, 771
BanI GGYRCC 2 cut(s) 591, 641
BanII GRGCYC 1 cut(s) 24
BbsI GAAGAC 2 cut(s) 473, 913
BbvI GCAGC 1 cut(s) 740
BccI CCATC 3 cut(s) 122, 367, 545
BciT130I CCWGG 4 cut(s) 332, 338, 610, 812
BciVI GTATCC 1 cut(s) 457
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 758, 964
BfaI CTAG 2 cut(s) 383, 471
BfmI CTRYAG 1 cut(s) 974
BfuI GTATCC 1 cut(s) 457
BglII AGATCT 1 cut(s) 942
BisI GCNGC 3 cut(s) 69, 677, 729
BlsI GCNGC 3 cut(s) 70, 678, 730
Bme1390I CCNGG 5 cut(s) 326, 332, 338, 610, 812
Bme18I GGWCC 4 cut(s) 122, 216, 284, 334
BmgT120I GGNCC 7 cut(s) 66, 122, 166, 216, 284, 328, 334
BmiI GGNNCC 4 cut(s) 23, 593, 643, 808
BmrFI CCNGG 5 cut(s) 326, 332, 338, 610, 812
BmsI GCATC 4 cut(s) 154, 357, 705, 727
BpiI GAAGAC 2 cut(s) 473, 913
BpmI CTGGAG 2 cut(s) 277, 459
BpuMI CCSGG 1 cut(s) 326
BsaJI CCNNGG 4 cut(s) 82, 330, 609, 810
BsaWI WCCGGW 1 cut(s) 124
Bsc4I CCNNNNNNNGG 7 cut(s) 63, 114, 163, 175, 293, 331, 337
Bse118I RCCGGY 2 cut(s) 56, 286
Bse1I ACTGG 2 cut(s) 260, 362
Bse3DI GCAATG 2 cut(s) 866, 905
BseAI TCCGGA 1 cut(s) 124
BseBI CCWGG 4 cut(s) 332, 338, 610, 812
BseDI CCNNGG 4 cut(s) 82, 330, 609, 810
BseGI GGATG 2 cut(s) 133, 718
BseLI CCNNNNNNNGG 7 cut(s) 63, 114, 163, 175, 293, 331, 337
BseMI GCAATG 2 cut(s) 866, 905
BseMII CTCAG 3 cut(s) 402, 584, 886
BseNI ACTGG 2 cut(s) 260, 362
BseXI GCAGC 1 cut(s) 740
BsgI GTGCAG 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 679
BshFI GGCC 7 cut(s) 44, 68, 167, 329, 341, 676, 771
BshNI GGYRCC 2 cut(s) 591, 641
BsiSI CCGG 5 cut(s) 25, 57, 125, 287, 326
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 7 cut(s) 63, 114, 163, 175, 293, 331, 337
BsmAI GTCTC 2 cut(s) 758, 964
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 7 cut(s) 44, 68, 167, 329, 341, 676, 771
Bsp1286I GDGCHC 1 cut(s) 24
Bsp13I TCCGGA 1 cut(s) 124
Bsp143I GATC 2 cut(s) 657, 942
BspACI CCGC 6 cut(s) 69, 115, 234, 677, 687, 838
BspANI GGCC 7 cut(s) 44, 68, 167, 329, 341, 676, 771
BspCNI CTCAG 3 cut(s) 403, 583, 885
BspEI TCCGGA 1 cut(s) 124
BspFNI CGCG 1 cut(s) 679
BspLI GGNNCC 4 cut(s) 23, 593, 643, 808
BspMAI CTGCAG 1 cut(s) 978
BspT107I GGYRCC 2 cut(s) 591, 641
BsrDI GCAATG 2 cut(s) 866, 905
BsrFI RCCGGY 2 cut(s) 56, 286
BsrI ACTGG 2 cut(s) 260, 362
BssAI RCCGGY 2 cut(s) 56, 286
BssECI CCNNGG 4 cut(s) 82, 330, 609, 810
BssMI GATC 2 cut(s) 657, 942
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 4 cut(s) 332, 338, 610, 812
Bst4CI ACNGT 1 cut(s) 919
Bst6I CTCTTC 1 cut(s) 977
BstAPI GCANNNNNTGC 1 cut(s) 973
BstC8I GCNNGC 2 cut(s) 232, 674
BstDEI CTNAG 3 cut(s) 411, 570, 872
BstF5I GGATG 2 cut(s) 133, 718
BstFNI CGCG 1 cut(s) 679
BstHHI GCGC 2 cut(s) 241, 764
BstKTI GATC 2 cut(s) 660, 945
BstMAI GTCTC 2 cut(s) 758, 964
BstMBI GATC 2 cut(s) 657, 942
BstMWI GCNNNNNNNGC 3 cut(s) 236, 768, 973
BstNI CCWGG 4 cut(s) 332, 338, 610, 812
BstNSI RCATGY 1 cut(s) 667
BstSCI CCNGG 5 cut(s) 324, 330, 336, 608, 810
BstSFI CTRYAG 1 cut(s) 974
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 1 cut(s) 740
BstV2I GAAGAC 2 cut(s) 473, 913
BstX2I RGATCY 1 cut(s) 942
BstYI RGATCY 1 cut(s) 942
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 7 cut(s) 44, 68, 167, 329, 341, 676, 771
BtsCI GGATG 2 cut(s) 133, 718
BtsIMutI CAGTG 2 cut(s) 369, 880
Cac8I GCNNGC 2 cut(s) 232, 674
CaiI CAGNNNCTG 2 cut(s) 337, 973
CfoI GCGC 2 cut(s) 241, 764
Cfr10I RCCGGY 2 cut(s) 56, 286
Cfr13I GGNCC 7 cut(s) 66, 122, 166, 216, 284, 328, 334
CpoI CGGWCCG 1 cut(s) 122
CseI GACGC 1 cut(s) 40
Csp6I GTAC 5 cut(s) 253, 378, 423, 592, 642
CspI CGGWCCG 1 cut(s) 122
CviAII CATG 5 cut(s) 451, 628, 655, 664, 732
CviQI GTAC 5 cut(s) 253, 378, 423, 592, 642
DdeI CTNAG 3 cut(s) 411, 570, 872
DpnI GATC 2 cut(s) 659, 944
DpnII GATC 2 cut(s) 657, 942
EaeI YGGCCR 3 cut(s) 339, 674, 769
Eam1104I CTCTTC 1 cut(s) 977
EarI CTCTTC 1 cut(s) 977
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 24
Eco47I GGWCC 4 cut(s) 122, 216, 284, 334
Eco57I CTGAAG 2 cut(s) 77, 744
EcoO109I RGGNCCY 1 cut(s) 334
EcoRII CCWGG 4 cut(s) 330, 336, 608, 810
EcoT14I CCWWGG 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 720
EcoT38I GRGCYC 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 5 cut(s) 454, 631, 658, 667, 735
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 749
FatI CATG 5 cut(s) 450, 627, 654, 663, 731
FauI CCCGC 1 cut(s) 845
Fnu4HI GCNGC 3 cut(s) 69, 677, 729
FokI GGATG 2 cut(s) 140, 705
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 3 cut(s) 69, 677, 729
FspBI CTAG 2 cut(s) 383, 471
GlaI GCGC 2 cut(s) 240, 763
GluI GCNGC 3 cut(s) 69, 677, 729
GsuI CTGGAG 2 cut(s) 277, 459
HaeIII GGCC 7 cut(s) 44, 68, 167, 329, 341, 676, 771
HapII CCGG 5 cut(s) 25, 57, 125, 287, 326
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 241, 764
Hin1II CATG 5 cut(s) 454, 631, 658, 667, 735
Hin6I GCGC 2 cut(s) 239, 762
HinP1I GCGC 2 cut(s) 239, 762
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 4 cut(s) 297, 358, 555, 575
HpaII CCGG 5 cut(s) 25, 57, 125, 287, 326
Hpy166II GTNNAC 2 cut(s) 108, 249
Hpy188I TCNGA 1 cut(s) 942
Hpy188III TCNNGA 7 cut(s) 95, 125, 157, 410, 463, 572, 722
Hpy8I GTNNAC 2 cut(s) 108, 249
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 919
HpyCH4IV ACGT 1 cut(s) 251
HpyCH4V TGCA 7 cut(s) 263, 458, 509, 711, 718, 967, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 768, 973
HpyF3I CTNAG 3 cut(s) 411, 570, 872
HpySE526I ACGT 1 cut(s) 251
Hsp92II CATG 5 cut(s) 454, 631, 658, 667, 735
HspAI GCGC 2 cut(s) 239, 762
Kpn2I TCCGGA 1 cut(s) 124
KpnI GGTACC 2 cut(s) 595, 645
Kzo9I GATC 2 cut(s) 657, 942
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 1 cut(s) 740
LweI GCATC 4 cut(s) 154, 357, 705, 727
MaeI CTAG 2 cut(s) 383, 471
MaeII ACGT 1 cut(s) 251
MaeIII GTNAC 1 cut(s) 913
MalI GATC 2 cut(s) 659, 944
MboI GATC 2 cut(s) 657, 942
MboII GAAGA 8 cut(s) 83, 220, 478, 775, 913, 970, 991, 994
MflI RGATCY 1 cut(s) 942
MhlI GDGCHC 1 cut(s) 24
MlsI TGGCCA 2 cut(s) 341, 771
MluCI AATT 3 cut(s) 704, 775, 994
MluNI TGGCCA 2 cut(s) 341, 771
MnlI CCTC 6 cut(s) 358, 434, 522, 674, 688, 959
Mox20I TGGCCA 2 cut(s) 341, 771
Mph1103I ATGCAT 1 cut(s) 720
MroI TCCGGA 1 cut(s) 124
MscI TGGCCA 2 cut(s) 341, 771
MseI TTAA 3 cut(s) 185, 537, 997
MslI CAYNNNNRTG 2 cut(s) 504, 857
Msp20I TGGCCA 2 cut(s) 341, 771
MspI CCGG 5 cut(s) 25, 57, 125, 287, 326
MspR9I CCNGG 5 cut(s) 326, 332, 338, 610, 812
MvaI CCWGG 4 cut(s) 332, 338, 610, 812
MvnI CGCG 1 cut(s) 679
MwoI GCNNNNNNNGC 3 cut(s) 236, 768, 973
NciI CCSGG 1 cut(s) 326
NdeII GATC 2 cut(s) 657, 942
NlaIII CATG 5 cut(s) 454, 631, 658, 667, 735
NlaIV GGNNCC 4 cut(s) 23, 593, 643, 808
NmuCI GTSAC 1 cut(s) 913
NsiI ATGCAT 1 cut(s) 720
NspI RCATGY 1 cut(s) 667
PciI ACATGT 1 cut(s) 663
PfeI GAWTC 4 cut(s) 297, 358, 555, 575
PflMI CCANNNNNTGG 2 cut(s) 175, 337
PkrI GCNGC 3 cut(s) 70, 678, 730
PpuMI RGGWCCY 1 cut(s) 334
PscI ACATGT 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 4 cut(s) 330, 336, 608, 810
PspGI CCWGG 4 cut(s) 330, 336, 608, 810
PspN4I GGNNCC 4 cut(s) 23, 593, 643, 808
PspPI GGNCC 7 cut(s) 66, 122, 166, 216, 284, 328, 334
PspPPI RGGWCCY 1 cut(s) 334
PstI CTGCAG 1 cut(s) 978
PstNI CAGNNNCTG 2 cut(s) 337, 973
PsuI RGATCY 1 cut(s) 942
RsaI GTAC 5 cut(s) 254, 379, 424, 593, 643
RsaNI GTAC 5 cut(s) 253, 378, 423, 592, 642
RseI CAYNNNNRTG 2 cut(s) 504, 857
Rsr2I CGGWCCG 1 cut(s) 122
RsrII CGGWCCG 1 cut(s) 122
SaqAI TTAA 3 cut(s) 185, 537, 997
SatI GCNGC 3 cut(s) 69, 677, 729
Sau3AI GATC 2 cut(s) 657, 942
Sau96I GGNCC 7 cut(s) 66, 122, 166, 216, 284, 328, 334
ScrFI CCNGG 5 cut(s) 326, 332, 338, 610, 812
SduI GDGCHC 1 cut(s) 24
SfaNI GCATC 4 cut(s) 154, 357, 705, 727
SfcI CTRYAG 1 cut(s) 974
SinI GGWCC 4 cut(s) 122, 216, 284, 334
SmiMI CAYNNNNRTG 2 cut(s) 504, 857
Sse9I AATT 3 cut(s) 704, 775, 994
SsiI CCGC 6 cut(s) 69, 115, 234, 677, 687, 838
SspMI CTAG 2 cut(s) 383, 471
StyD4I CCNGG 5 cut(s) 324, 330, 336, 608, 810
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 919
TaiI ACGT 1 cut(s) 254
TaqI TCGA 2 cut(s) 141, 300
TaqII GACCGA 1 cut(s) 110
TasI AATT 3 cut(s) 704, 775, 994
TatI WGTACW 1 cut(s) 422
TauI GCSGC 2 cut(s) 71, 679
TfiI GAWTC 4 cut(s) 297, 358, 555, 575
Tru1I TTAA 3 cut(s) 185, 537, 997
Tru9I TTAA 3 cut(s) 185, 537, 997
TscAI CASTG 2 cut(s) 369, 880
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 1 cut(s) 728
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 2 cut(s) 644, 769
TspRI CASTG 2 cut(s) 369, 880
Van91I CCANNNNNTGG 2 cut(s) 175, 337
VpaK11BI GGWCC 4 cut(s) 122, 216, 284, 334
XapI RAATTY 1 cut(s) 775
XceI RCATGY 1 cut(s) 667
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XspI CTAG 2 cut(s) 383, 471
Zsp2I ATGCAT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.