Rh5AG028800

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001481
Physical Location & Seq
Forward (+)
2000549 .. 2005937
5389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG028800.1

Sequence Viewer

Length: 1521 bp
ATGGACTACTCCAGTGATAGTGATGAGCTCTCTGAACCCATCTTGACTGACAAAAGATCTTCATCTGTTCAACTAGTGAGCTCGGAACTGGAAGAAACGCTATCCAACACCGAGCTTCCGTACTTCCACCGTCTTAGACGTGCCACGTGGGTCGAACTGAAGATTCTCTTCCGCCTTGCTGCTCCGGCAGTGGTGGTTTACTTGCTAAACAACGTTATTTCCATGTCCACCCAAATCTACTGCGGGCATCTCGGCAATCTCGAACTCGCTGCCTCTTCTCTTGGCAACACCGGCATCCAAGTTTTTGCCTACGGCCTCATGCTTGGAATGGGAAGTGCGGTGGAAACGCTCTGTGGTCAAGCCTATGGAGCAAAGAAATATGACATGCTAGGCGTGTATCTGCAGCGTTCAACGATCCTGCTAATGGCAACTGGGATCCCAGTTATGTTCATCTACATCTTCTCCGAGTCCATCTTGATTGCCCTAGGCGAATCGGCCACCATTGCCTCTGCAGCCGCAATCTTCGTTTACGGACTTATCCCCCAAATCTTTGCCTACGCTTGCAATTTCCCCATACAAAAATTCCTCCAGGCTCAGAGCATCGTGTTCCCGAGCGCGTACATATCCGCGGCTGCCCTCGTGGTGCATGTGGTGCTGAGTTGGGTAGTGATTTATAAGTTGGGATGGGGCTTGTTAGGCGCATCGCTGATGTTGAGTTTTTCGTGGTGGATTATAGTGGTGGCGCAGTTTGTTTACATATTGCTGAGTCCAAGGTGTAAACGTACTTGGACAGGTTTTAGCATGCAGGCTTTTTCTGGGCTTTGGACTTTTCTGAAGCTATCTGTTGCATCTGCCGTAATGCTTTGCCTCGAGACTTGGTACTATCAGATTATCGTTTTGATTGCTGGGTTGCTTGAAGACGCTGAAATTGCCTTGGACGCTCTCTCTGTGTGCATGACCATATCTGGATGGGTGTTCATGGTTGCTGTGGGTTTCAATGCTGCTGCAAGTGTAAGGATTGGGAATGAGCTCGGGGCGGGACATCCAAAGTCGGCCGCATTTGCAGTAATAATAGTGACATCAACCTCGTTCATCATCTCAGTAGTGTGTGCCATAGTTGTGCTTGCACTACGAAATGTCATAAGCTACGCGTTCACCGAGGGCGAGACTGTGTCCAATGCAGTCTCAGCCCTATCTCCTTACCTTGCTGTCTCTATTATGCTCAACGGCATCCAACCTGTTTTGTCTGGGGTGGCTGTTGGGTGTGGATGGCAAGCATTTGTGGCATATGTTAACGTCGGATGTTACTACATTATTGGTATTCCTTTGGGTTGTGTTCTTGGCTTCAAGTTTGACTTTGGAGCCCAGGGAATATGGTCTGGAATGATAGGAGGGACATTTATACAGACGGCGATCTTATTGTGGGTCACACTTCGGACTGATTGGAATAAAGAGGTCGCAAATGCAAAGAATCGTTTGGACAAGTGGGATGACAAGAAGGAGCCTCTTCTCACACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

506

Amino Acids

54.99

Weight (kDa)

5.67

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 62 - 222 1.5e-34 MatE
MatE PF01554 284 - 444 5.4e-30 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AccII CGCG 3 cut(s) 617, 629, 1151
AciI CCGC 8 cut(s) 172, 243, 338, 516, 627, 629, 1037, 1056
AclI AACGTT 1 cut(s) 213
AclWI GGATC 3 cut(s) 409, 430, 443
AcoI YGGCCR 2 cut(s) 495, 1053
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 2 cut(s) 179, 854
AcvI CACGTG 1 cut(s) 147
AfaI GTAC 4 cut(s) 122, 620, 784, 881
AfiI CCNNNNNNNGG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 1149
AgsI TTSAA 5 cut(s) 71, 411, 917, 997, 1348
AhlI ACTAGT 1 cut(s) 73
AjiI CACGTC 1 cut(s) 140
AjnI CCWGG 2 cut(s) 588, 1365
AluBI AGCT 6 cut(s) 28, 81, 115, 838, 1030, 1146
AluI AGCT 6 cut(s) 28, 81, 115, 838, 1030, 1146
Alw21I GWGCWC 3 cut(s) 30, 83, 1032
Alw26I GTCTC 4 cut(s) 866, 1160, 1189, 1216
AlwI GGATC 3 cut(s) 409, 430, 443
Ama87I CYCGRG 3 cut(s) 610, 869, 1031
AoxI GGCC 3 cut(s) 313, 495, 1053
ApeKI GCWGC 7 cut(s) 179, 269, 403, 512, 632, 1001, 1004
ApoI RAATTY 1 cut(s) 581
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 3 cut(s) 617, 701, 745
AsuHPI GGTGA 1 cut(s) 1147
AvaI CYCGRG 3 cut(s) 610, 869, 1031
AvrII CCTAGG 1 cut(s) 484
BamHI GGATCC 1 cut(s) 435
BanII GRGCYC 4 cut(s) 30, 83, 1032, 1366
BauI CACGAG 1 cut(s) 638
BbrPI CACGTG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 3 cut(s) 30, 83, 1032
BbvI GCAGC 7 cut(s) 166, 256, 415, 524, 619, 988, 991
BccI CCATC 5 cut(s) 47, 479, 678, 963, 1263
BceAI ACGGC 4 cut(s) 328, 839, 1243, 1425
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 590, 1367
BcoDI GTCTC 4 cut(s) 866, 1160, 1189, 1216
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 3 cut(s) 74, 389, 485
BfmI CTRYAG 2 cut(s) 401, 510
BglII AGATCT 1 cut(s) 56
BlnI CCTAGG 1 cut(s) 484
Bme1390I CCNGG 2 cut(s) 590, 1367
BmeT110I CYCGRG 3 cut(s) 610, 869, 1031
BmgBI CACGTC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 437, 1363, 1503
BmrFI CCNGG 2 cut(s) 590, 1367
BmrI ACTGGG 2 cut(s) 434, 441
BmsI GCATC 6 cut(s) 256, 303, 609, 710, 857, 1239
BmuI ACTGGG 2 cut(s) 434, 441
BpiI GAAGAC 1 cut(s) 924
BpmI CTGGAG 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 61
BsaJI CCNNGG 7 cut(s) 484, 627, 770, 933, 1158, 1365, 1366
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 4 cut(s) 12, 93, 436, 440
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 1 cut(s) 61
BseBI CCWGG 2 cut(s) 590, 1367
BseDI CCNNGG 7 cut(s) 484, 627, 770, 933, 1158, 1365, 1366
BseGI GGATG 8 cut(s) 294, 689, 974, 1042, 1230, 1274, 1307, 1495
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 5 cut(s) 608, 647, 755, 1113, 1200
BseNI ACTGG 4 cut(s) 12, 93, 436, 440
BseX3I CGGCCG 1 cut(s) 1053
BseXI GCAGC 7 cut(s) 166, 256, 415, 524, 619, 988, 991
BseYI CCCAGC 1 cut(s) 905
Bsh1236I CGCG 3 cut(s) 617, 629, 1151
Bsh1285I CGRYCG 1 cut(s) 1056
BshFI GGCC 3 cut(s) 315, 497, 1055
BsiEI CGRYCG 1 cut(s) 1056
BsiHKAI GWGCWC 3 cut(s) 30, 83, 1032
BsiHKCI CYCGRG 3 cut(s) 610, 869, 1031
BsiSI CCGG 2 cut(s) 185, 291
BslFI GGGAC 2 cut(s) 1053, 1408
BslI CCNNNNNNNGG 1 cut(s) 424
BsmAI GTCTC 4 cut(s) 866, 1160, 1189, 1216
BsmFI GGGAC 2 cut(s) 1053, 1408
BsnI GGCC 3 cut(s) 315, 497, 1055
BsoBI CYCGRG 3 cut(s) 610, 869, 1031
Bsp1286I GDGCHC 4 cut(s) 30, 83, 1032, 1366
Bsp143I GATC 4 cut(s) 56, 414, 435, 1413
BspACI CCGC 8 cut(s) 172, 243, 338, 516, 627, 629, 1037, 1056
BspANI GGCC 3 cut(s) 315, 497, 1055
BspCNI CTCAG 5 cut(s) 607, 648, 756, 1112, 1199
BspFNI CGCG 3 cut(s) 617, 629, 1151
BspLI GGNNCC 3 cut(s) 437, 1363, 1503
BspMAI CTGCAG 2 cut(s) 405, 514
BspPI GGATC 3 cut(s) 409, 430, 443
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 4 cut(s) 12, 93, 436, 440
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 7 cut(s) 484, 627, 770, 933, 1158, 1365, 1366
BssMI GATC 4 cut(s) 56, 414, 435, 1413
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 3 cut(s) 484, 770, 933
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 2 cut(s) 590, 1367
Bst4CI ACNGT 2 cut(s) 131, 1171
Bst6I CTCTTC 3 cut(s) 173, 280, 1512
BstAPI GCANNNNNTGC 1 cut(s) 652
BstBAI YACGTR 1 cut(s) 147
BstC8I GCNNGC 6 cut(s) 245, 562, 803, 807, 1125, 1275
BstDEI CTNAG 6 cut(s) 134, 594, 656, 764, 1099, 1186
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 8 cut(s) 294, 689, 974, 1042, 1230, 1274, 1307, 1495
BstFNI CGCG 3 cut(s) 617, 629, 1151
BstHHI GCGC 3 cut(s) 617, 701, 745
BstKTI GATC 4 cut(s) 59, 417, 438, 1416
BstMAI GTCTC 4 cut(s) 866, 1160, 1189, 1216
BstMBI GATC 4 cut(s) 56, 414, 435, 1413
BstMCI CGRYCG 1 cut(s) 1056
BstNI CCWGG 2 cut(s) 590, 1367
BstNSI RCATGY 3 cut(s) 388, 650, 805
BstSCI CCNGG 2 cut(s) 588, 1365
BstSFI CTRYAG 2 cut(s) 401, 510
BstUI CGCG 3 cut(s) 617, 629, 1151
BstV1I GCAGC 7 cut(s) 166, 256, 415, 524, 619, 988, 991
BstV2I GAAGAC 1 cut(s) 924
BstX2I RGATCY 2 cut(s) 56, 435
BstYI RGATCY 2 cut(s) 56, 435
BstZI CGGCCG 1 cut(s) 1053
BsuRI GGCC 3 cut(s) 315, 497, 1055
BtgI CCRYGG 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 8 cut(s) 294, 689, 974, 1042, 1230, 1274, 1307, 1495
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 19, 195
Cac8I GCNNGC 6 cut(s) 245, 562, 803, 807, 1125, 1275
CfoI GCGC 3 cut(s) 617, 701, 745
Cfr10I RCCGGY 1 cut(s) 290
Cfr42I CCGCGG 1 cut(s) 630
CseI GACGC 2 cut(s) 929, 947
Csp6I GTAC 4 cut(s) 121, 619, 783, 880
CviAII CATG 7 cut(s) 223, 319, 385, 647, 802, 955, 979
CviQI GTAC 4 cut(s) 121, 619, 783, 880
DdeI CTNAG 6 cut(s) 134, 594, 656, 764, 1099, 1186
DpnI GATC 4 cut(s) 58, 416, 437, 1415
DpnII GATC 4 cut(s) 56, 414, 435, 1413
EaeI YGGCCR 2 cut(s) 495, 1053
EagI CGGCCG 1 cut(s) 1053
Eam1104I CTCTTC 3 cut(s) 173, 280, 1512
EarI CTCTTC 3 cut(s) 173, 280, 1512
EciI GGCGGA 1 cut(s) 161
Ecl136II GAGCTC 3 cut(s) 28, 81, 1030
EclXI CGGCCG 1 cut(s) 1053
Eco130I CCWWGG 3 cut(s) 484, 770, 933
Eco24I GRGCYC 4 cut(s) 30, 83, 1032, 1366
Eco52I CGGCCG 1 cut(s) 1053
Eco53kI GAGCTC 3 cut(s) 28, 81, 1030
Eco57I CTGAAG 2 cut(s) 179, 854
Eco72I CACGTG 1 cut(s) 147
Eco88I CYCGRG 3 cut(s) 610, 869, 1031
EcoICRI GAGCTC 3 cut(s) 28, 81, 1030
EcoRII CCWGG 2 cut(s) 588, 1365
EcoT14I CCWWGG 3 cut(s) 484, 770, 933
EcoT38I GRGCYC 4 cut(s) 30, 83, 1032, 1366
ErhI CCWWGG 3 cut(s) 484, 770, 933
FaeI CATG 7 cut(s) 226, 322, 388, 650, 805, 958, 982
FalI AAGNNNNNCTT 4 cut(s) 152, 184, 1340, 1372
FaqI GGGAC 2 cut(s) 1053, 1408
FatI CATG 7 cut(s) 222, 318, 384, 646, 801, 954, 978
FauI CCCGC 2 cut(s) 236, 1030
FauNDI CATATG 1 cut(s) 1288
FokI GGATG 8 cut(s) 281, 696, 981, 1029, 1217, 1281, 1314, 1502
FriOI GRGCYC 4 cut(s) 30, 83, 1032, 1366
FspBI CTAG 3 cut(s) 74, 389, 485
GlaI GCGC 3 cut(s) 616, 700, 744
GsaI CCCAGC 1 cut(s) 909
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 3 cut(s) 315, 497, 1055
HapII CCGG 2 cut(s) 185, 291
HgaI GACGC 2 cut(s) 929, 947
HhaI GCGC 3 cut(s) 617, 701, 745
Hin1II CATG 7 cut(s) 226, 322, 388, 650, 805, 958, 982
Hin6I GCGC 3 cut(s) 615, 699, 743
HinP1I GCGC 3 cut(s) 615, 699, 743
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HinfI GANTC 5 cut(s) 163, 467, 491, 766, 1471
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 2 cut(s) 185, 291
HphI GGTGA 1 cut(s) 1147
Hpy166II GTNNAC 7 cut(s) 199, 228, 529, 754, 779, 1155, 1294
Hpy188I TCNGA 8 cut(s) 34, 85, 466, 597, 834, 888, 1301, 1437
Hpy188III TCNNGA 7 cut(s) 43, 260, 475, 610, 871, 966, 1380
Hpy8I GTNNAC 7 cut(s) 199, 228, 529, 754, 779, 1155, 1294
Hpy99I CGWCG 1 cut(s) 1301
HpyAV CCTTC 1 cut(s) 1492
HpyCH4III ACNGT 2 cut(s) 131, 1171
HpyCH4IV ACGT 5 cut(s) 139, 146, 213, 781, 1296
HpyF3I CTNAG 6 cut(s) 134, 594, 656, 764, 1099, 1186
HpySE526I ACGT 5 cut(s) 139, 146, 213, 781, 1296
Hsp92II CATG 7 cut(s) 226, 322, 388, 650, 805, 958, 982
HspAI GCGC 3 cut(s) 615, 699, 743
KspAI GTTAAC 1 cut(s) 1294
KspI CCGCGG 1 cut(s) 630
Kzo9I GATC 4 cut(s) 56, 414, 435, 1413
LmnI GCTCC 4 cut(s) 187, 368, 1361, 1501
Lsp1109I GCAGC 7 cut(s) 166, 256, 415, 524, 619, 988, 991
LweI GCATC 6 cut(s) 256, 303, 609, 710, 857, 1239
MaeI CTAG 3 cut(s) 74, 389, 485
MaeII ACGT 5 cut(s) 139, 146, 213, 781, 1296
MaeIII GTNAC 3 cut(s) 1075, 1304, 1426
MalI GATC 4 cut(s) 58, 416, 437, 1415
MboI GATC 4 cut(s) 56, 414, 435, 1413
MboII GAAGA 9 cut(s) 51, 104, 160, 172, 267, 451, 514, 929, 1499
MflI RGATCY 2 cut(s) 56, 435
MhlI GDGCHC 4 cut(s) 30, 83, 1032, 1366
MluCI AATT 3 cut(s) 565, 581, 927
MluI ACGCGT 1 cut(s) 1149
MlyI GAGTC 2 cut(s) 476, 775
MmeI TCCRAC 3 cut(s) 129, 1258, 1279
MseI TTAA 1 cut(s) 1293
MslI CAYNNNNRTG 1 cut(s) 1118
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 2 cut(s) 185, 291
MspR9I CCNGG 2 cut(s) 590, 1367
MvaI CCWGG 2 cut(s) 590, 1367
MvnI CGCG 3 cut(s) 617, 629, 1151
NdeI CATATG 1 cut(s) 1288
NdeII GATC 4 cut(s) 56, 414, 435, 1413
NlaIII CATG 7 cut(s) 226, 322, 388, 650, 805, 958, 982
NlaIV GGNNCC 3 cut(s) 437, 1363, 1503
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 1075, 1426
NspI RCATGY 3 cut(s) 388, 650, 805
PaeI GCATGC 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 869
PasI CCCWGGG 1 cut(s) 1366
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 1155
PfeI GAWTC 3 cut(s) 163, 491, 1471
PflFI GACNNNGTC 1 cut(s) 1171
PleI GAGTC 2 cut(s) 475, 774
PmaCI CACGTG 1 cut(s) 147
PmlI CACGTG 1 cut(s) 147
PpsI GAGTC 2 cut(s) 475, 774
Ppu21I YACGTR 1 cut(s) 147
PsiI TTATAA 1 cut(s) 675
Psp124BI GAGCTC 3 cut(s) 30, 83, 1032
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 588, 1365
PspCI CACGTG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 905
PspGI CCWGG 2 cut(s) 588, 1365
PspN4I GGNNCC 3 cut(s) 437, 1363, 1503
PstI CTGCAG 2 cut(s) 405, 514
PsuI RGATCY 2 cut(s) 56, 435
PsyI GACNNNGTC 1 cut(s) 1171
RsaI GTAC 4 cut(s) 122, 620, 784, 881
RsaNI GTAC 4 cut(s) 121, 619, 783, 880
RseI CAYNNNNRTG 1 cut(s) 1118
SacI GAGCTC 3 cut(s) 30, 83, 1032
SacII CCGCGG 1 cut(s) 630
SaqAI TTAA 1 cut(s) 1293
Sau3AI GATC 4 cut(s) 56, 414, 435, 1413
SchI GAGTC 2 cut(s) 476, 775
ScrFI CCNGG 2 cut(s) 590, 1367
SduI GDGCHC 4 cut(s) 30, 83, 1032, 1366
SfaNI GCATC 6 cut(s) 256, 303, 609, 710, 857, 1239
SfcI CTRYAG 2 cut(s) 401, 510
Sfr274I CTCGAG 1 cut(s) 869
Sfr303I CCGCGG 1 cut(s) 630
SgrBI CCGCGG 1 cut(s) 630
SlaI CTCGAG 1 cut(s) 869
SmiMI CAYNNNNRTG 1 cut(s) 1118
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
SpeI ACTAGT 1 cut(s) 73
SphI GCATGC 1 cut(s) 805
Sse9I AATT 3 cut(s) 565, 581, 927
SsiI CCGC 8 cut(s) 172, 243, 338, 516, 627, 629, 1037, 1056
SspMI CTAG 3 cut(s) 74, 389, 485
SstI GAGCTC 3 cut(s) 30, 83, 1032
StyD4I CCNGG 2 cut(s) 588, 1365
StyI CCWWGG 3 cut(s) 484, 770, 933
TaaI ACNGT 2 cut(s) 131, 1171
TaiI ACGT 5 cut(s) 142, 149, 216, 784, 1299
TaqI TCGA 3 cut(s) 153, 261, 870
TasI AATT 3 cut(s) 565, 581, 927
TauI GCSGC 3 cut(s) 518, 632, 1058
TfiI GAWTC 3 cut(s) 163, 491, 1471
Tru1I TTAA 1 cut(s) 1293
Tru9I TTAA 1 cut(s) 1293
TscAI CASTG 2 cut(s) 19, 195
TseFI GTSAC 2 cut(s) 1075, 1426
TseI GCWGC 7 cut(s) 179, 269, 403, 512, 632, 1001, 1004
Tsp45I GTSAC 2 cut(s) 1075, 1426
TspDTI ATGAA 4 cut(s) 51, 439, 967, 1081
TspGWI ACGGA 2 cut(s) 108, 546
TspRI CASTG 2 cut(s) 19, 195
Tth111I GACNNNGTC 1 cut(s) 1171
XapI RAATTY 1 cut(s) 581
XceI RCATGY 3 cut(s) 388, 650, 805
XhoI CTCGAG 1 cut(s) 869
XmaJI CCTAGG 1 cut(s) 484
XspI CTAG 3 cut(s) 74, 389, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.