MD06G1055600.v1.1

DWNN domain, A CCHC-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001377
Physical Location & Seq
Forward (+)
8314083 .. 8320866
6784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1055600.v1.1.491

Sequence Viewer

Length: 2715 bp
ATGGCGGTGTATTATAAATTTAAGAGTGCAAAAGATTATGATTCTATTCCTATGGACGGCCCGTTCATCGCAGTTGGTATTTTGAAAGAAAAAATATATGAATCCAAGCACTTAGGGCGGGGTACTGATTACGACCTCGTCGTCACCAATGCCCAGACCAATGAAGAGTATCTTGATGAAGCAATGTTGATTCCAAAAAATACGTCAGTTTTAATTCGCCGTGTACCTGGAAGGCCTCCAATGCCTATTGTTACTGATTCAGAGCGAAAGATGGAAGATAAGGTGGAGTACACTGAACCAGAAAGGACTAGCTTTCTGGCGGCTGAATCATCTGCCACGAAATATCCTGGTGATCCAGATTGGGATGATTATGGGGATGATTTGTATGAAATTCCTGAAGTGATGACAGTGCAGACAAGTTATCAGGCTCCGGAAGCGCAGCCAACTGATAAAGCTGATGAAGATAGCAAGATTAAGGCTTTAATCGACACTCCAGCCTTGGCATGGCAACACCAAGGTCCTGATGGTTTTGGTCCTGGTAGAGGTTTTGGCAGGGGTGGAAGAATGATGAATGGACGTGGTATTGTAGGGGGCTTTGAGAGGAAAACACCTCCACAGGGCTATATTTGTCATAGGTGTAAGGTGCCTGGGCATTATATTCAGCACTGCCCTACGAATGGTGATCCAAATTTTGACGTTAAAAGAGTGAAGCCACCCACTGGAATTCCTAAGTCCATGCTGATGGCAAACCCGGATGGCTCATATAAGTTGCCAAGTGGTGATAAAGCTGTATTGAGGCCAAATGAGGCTGCTTTTGAGAAAGAGATTGAAGGTTTACCATCTACGCGTTCTGTTGGTGATCTACCCCCCGAGCTACACTGCCCGTTGTGCAAAGAAGTGATGAAGGATGCTGTGCTAACGAGCAAGTGTTGCTTTAATAGCTTTTGTGATAAGTGTATCAGGAACTACATTATGTCCAAGTCAGTGTGTGTCTGTGGGGCTACAAATACACTGGCAGACGATCTACTGCCAAATAAGACACTGAGAGATACAATCAATCGCATCTTGGAGTCTGGTGGTAACAGTAGTGCTGACAATGCTGGGAGTACTTTCCAAGTTCAAGATATGGAGTCTGCTCGCTGTCCACAACCCAAGATTCCTTCCCCTACAGTATCTGCTGCATCAAAAGGAGATGAAAAGCAGTTACCTCAGAATGAAGAACCTCCAAAGTTTCCTGAATCCGAAGATGACGTAAAGCCTGTTCTTCCCCAGCAGCAGATTTTAGAAAGAGTGAGAACTACAATAGTAGCTGATGCATCTGAAGCCACGCGTGAGTCAATGAGTGTGAAGGAACCAGCGTCACAAGGTAGTATTCCACTGGTTGAGGAAGAAGTGCAGCAAAGGATGGCTTCTGGGGATGCAGGAAAGAAAAGGAAAAAGAAGAAAATCCGTGTGCCTGTGAATGACATGCAATGGAGAAACCCTCAAGATCTTGCAGCCGAAAACTATATGATGCCTATGGGCCCCGCTGCTTATAATCCGTACTGGAATGGCATGCAACCTGGCATGGGCATGGAAGGATATATGACATCATATGGTGGTCCGATGCCATATATGGGTTATGGTCCCATTGACATGCCATTTGGGGGTCTTGGTCCACAGGACCCATTTGGTGCTCAAGGTTATATGATGCCCATGGTTCCGCCTCAGAGGGATCCTGCGGACTTTGGTATGGGCATGAATGGGATGAATGGGATGAATGGGATGAATGGACTCCCCATCATGAGCAAAGAAGAATTTGAGGCTCGGAAAGCTGATTTGAGAAGGAAGCGTGAAAATGAAAGACAGGGTCAAAGCCGGGAGTTCTCCAAAGACAGGGAATACAGAAGGGAAGTTAGCAGCGGCGGCGATGGTCCTCCAATGAAATCCAAATCTAAGTCGTTGGCCCGATCACCAAGCCCAGACTATCCTAATCAGCACCGCCGTCACCGACGCGAGAAGCAATCTCCAGAACGCAGGCCCTCACGTGACCCACGTGACCTTGAGCCACAGCCGCCTCCCCGTCCTCCAAAGAGAAAATCCGAGCAGCACCACCACGACAGGGAACGCGAGCGGGACCGAGATCGAGAACGCGAGAGGGAGCACGACCATGAAGAGGATCATCACCGCCAGAAACGACACCGTTCTGAGTCCTCCTCGGCCAAACCTTCCGCCCAAACGACAGCATCGGCGAAACCTGCCTCCTCTGCAGCATCAGCTGCTGCGGCAGAAGCGGCGGCCGCGGCGGAGCGGAAGCACAAGGCAAGCGTGTTCTCTCGCATAAGCTTTCCCGAGGGAGAAGTGAGCAAGAAGCGGAAGGCCTCTTCTTCCTCCACTGCTTCTGCCACCGCTCCTTCTCCGGCGGCGGAGGATACCAAACCGTCGGCGCATCACCATCACAAGGAGTCGTCGTCGTCGTCCTTGGCGAATGGGTTCTACGACGAGTACAAGTCGTCGTCGACGGCCAAGGCTGCGAGCAGCGGAACTGGAAGCAGAAAGACTATGATCAGTGCTGCTTCCATGGAGTACGAGTCGAGCGACGACGATCGGCACTTCAAGAGGAAGCCGTCGAGGTACGAGGCGTCACCGCCACAGGCCGCCGAGCAGGAAGAGCCCTCCGGGCACTCGAGAGGAGGCAGGGATCGGGATCGGGATCGGGATCGCAGGCAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005815 GO:0005829 GO:0005856 GO:0006275 GO:0006325 GO:0006464 GO:0006479 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0006979 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009056 GO:0009057 GO:0009414 GO:0009415 GO:0009628 GO:0009635 GO:0009636 GO:0009651 GO:0009743 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010555 GO:0010556 GO:0015630 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016607 GO:0016740 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032991 GO:0033554 GO:0034969 GO:0034971 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046677 GO:0046686 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051276 GO:0051603 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0072756 GO:0080090 GO:0080134 GO:0097237 GO:0140096 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902882 GO:1902883 GO:1902884 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DWNN zf-CCHC_2

Protein Analysis

905

Amino Acids

100.4

Weight (kDa)

8.06

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DWNN PF08783 3 - 76 5.4e-30 DWNN domain
zf-CCHC_2 PF13696 205 - 225 5.1e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 1536
AasI GACNNNNNNGTC 1 cut(s) 140
Acc36I ACCTGC 1 cut(s) 2245
AccB1I GGYRCC 1 cut(s) 643
AccB7I CCANNNNNTGG 1 cut(s) 719
AccBSI CCGCTC 3 cut(s) 2113, 2290, 2390
AccI GTMKAC 1 cut(s) 2498
AccII CGCG 6 cut(s) 847, 1330, 1995, 2109, 2133, 2282
AccIII TCCGGA 1 cut(s) 430
AclWI GGATC 9 cut(s) 347, 677, 1709, 1722, 2166, 2688, 2694, 2700, 2706
AcoI YGGCCR 3 cut(s) 2199, 2277, 2502
AcsI RAATTY 5 cut(s) 17, 390, 688, 723, 1796
AcuI CTGAAG 2 cut(s) 417, 1341
AcvI CACGTG 2 cut(s) 2027, 2036
AcyI GRCGYC 1 cut(s) 2621
AfaI GTAC 8 cut(s) 124, 225, 290, 1108, 1544, 2486, 2567, 2615
AflIII ACRYGT 2 cut(s) 845, 1328
AgsI TTSAA 4 cut(s) 85, 830, 1121, 2596
AjiI CACGTC 1 cut(s) 578
AjnI CCWGG 5 cut(s) 226, 346, 535, 646, 1561
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 9 cut(s) 312, 455, 788, 874, 942, 1310, 1814, 2258, 2325
AluI AGCT 9 cut(s) 312, 455, 788, 874, 942, 1310, 1814, 2258, 2325
Alw21I GWGCWC 2 cut(s) 1678, 2145
AlwI GGATC 9 cut(s) 347, 677, 1709, 1722, 2166, 2688, 2694, 2700, 2706
AlwNI CAGNNNCTG 2 cut(s) 1175, 2261
Ama87I CYCGRG 3 cut(s) 869, 2330, 2665
Aor13HI TCCGGA 1 cut(s) 430
ApaI GGGCCC 1 cut(s) 1526
ApoI RAATTY 5 cut(s) 17, 390, 688, 723, 1796
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 1625, 1657
Asp700I GAANNNNTTC 1 cut(s) 2471
AspLEI GCGC 2 cut(s) 439, 2428
AsuC2I CCSGG 3 cut(s) 752, 1859, 2659
AvaI CYCGRG 3 cut(s) 869, 2330, 2665
AvaII GGWCC 8 cut(s) 518, 533, 1601, 1625, 1655, 1663, 1913, 2116
BaeGI GKGCMC 2 cut(s) 1526, 2664
BamHI GGATCC 1 cut(s) 1714
BanI GGYRCC 1 cut(s) 643
BanII GRGCYC 2 cut(s) 1526, 2655
BbrPI CACGTG 2 cut(s) 2027, 2036
Bbv12I GWGCWC 2 cut(s) 1678, 2145
BccI CCATC 9 cut(s) 265, 518, 736, 749, 847, 1399, 1787, 1904, 2442
BceAI ACGGC 5 cut(s) 73, 204, 1968, 2517, 2590
BciT130I CCWGG 5 cut(s) 228, 348, 537, 648, 1563
BciVI GTATCC 1 cut(s) 2404
BclI TGATCA 1 cut(s) 2544
BcnI CCSGG 3 cut(s) 752, 1859, 2659
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 2 cut(s) 1167, 2247
BfuAI ACCTGC 1 cut(s) 2245
BfuI GTATCC 1 cut(s) 2404
BglI GCCNNNNNGGC 1 cut(s) 2659
BglII AGATCT 1 cut(s) 1489
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 8 cut(s) 228, 348, 537, 648, 752, 1563, 1859, 2659
Bme18I GGWCC 8 cut(s) 518, 533, 1601, 1625, 1655, 1663, 1913, 2116
BmeT110I CYCGRG 3 cut(s) 869, 2330, 2665
BmgBI CACGTC 1 cut(s) 578
BmrFI CCNGG 8 cut(s) 228, 348, 537, 648, 752, 1563, 1859, 2659
BplI GAGNNNNNCTC 4 cut(s) 1468, 1500, 2180, 2212
BpmI CTGGAG 2 cut(s) 477, 1992
BpuEI CTTGAG 3 cut(s) 1470, 1662, 2063
BpuMI CCSGG 3 cut(s) 752, 1859, 2659
BsaAI YACGTR 2 cut(s) 2027, 2036
BsaBI GATNNNNATC 3 cut(s) 2685, 2691, 2697
BsaHI GRCGYC 1 cut(s) 2621
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 4 cut(s) 2435, 2465, 2555, 2585
Bse1I ACTGG 5 cut(s) 724, 1017, 1383, 1550, 2530
Bse3DI GCAATG 2 cut(s) 189, 1478
Bse8I GATNNNNATC 3 cut(s) 2685, 2691, 2697
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 5 cut(s) 228, 348, 537, 648, 1563
BseGI GGATG 9 cut(s) 370, 382, 760, 913, 1410, 1423, 1752, 1761, 1770
BseJI GATNNNNATC 3 cut(s) 2685, 2691, 2697
BseMI GCAATG 2 cut(s) 189, 1478
BseMII CTCAG 4 cut(s) 1034, 1223, 1721, 2178
BseNI ACTGG 5 cut(s) 724, 1017, 1383, 1550, 2530
BseRI GAGGAG 3 cut(s) 2185, 2233, 2685
BseSI GKGCMC 2 cut(s) 1526, 2664
BseX3I CGGCCG 1 cut(s) 2277
BseYI CCCAGC 2 cut(s) 1100, 1269
BsgI GTGCAG 2 cut(s) 431, 1415
Bsh1236I CGCG 6 cut(s) 847, 1330, 1995, 2109, 2133, 2282
Bsh1285I CGRYCG 2 cut(s) 2280, 2587
BshNI GGYRCC 1 cut(s) 643
BsiEI CGRYCG 2 cut(s) 2280, 2587
BsiHKAI GWGCWC 2 cut(s) 1678, 2145
BsiHKCI CYCGRG 3 cut(s) 869, 2330, 2665
BsiSI CCGG 5 cut(s) 431, 752, 1858, 2399, 2658
BslFI GGGAC 2 cut(s) 1611, 2129
BsmFI GGGAC 2 cut(s) 1611, 2129
BsoBI CYCGRG 3 cut(s) 869, 2330, 2665
Bsp120I GGGCCC 1 cut(s) 1522
Bsp1286I GDGCHC 5 cut(s) 1526, 1678, 2145, 2655, 2664
Bsp13I TCCGGA 1 cut(s) 430
Bsp19I CCATGG 2 cut(s) 1695, 2559
BspCNI CTCAG 4 cut(s) 1035, 1222, 1720, 2179
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 6 cut(s) 847, 1330, 1995, 2109, 2133, 2282
BspHI TCATGA 1 cut(s) 1782
BspMAI CTGCAG 1 cut(s) 2251
BspMI ACCTGC 1 cut(s) 2245
BspPI GGATC 9 cut(s) 347, 677, 1709, 1722, 2166, 2688, 2694, 2700, 2706
BspQI GCTCTTC 1 cut(s) 2643
BspT107I GGYRCC 1 cut(s) 643
BsrBI CCGCTC 3 cut(s) 2113, 2290, 2390
BsrDI GCAATG 2 cut(s) 189, 1478
BsrI ACTGG 5 cut(s) 724, 1017, 1383, 1550, 2530
BssNI GRCGYC 1 cut(s) 2621
BssT1I CCWWGG 6 cut(s) 498, 514, 1695, 2460, 2505, 2559
Bst2UI CCWGG 5 cut(s) 228, 348, 537, 648, 1563
Bst4CI ACNGT 5 cut(s) 409, 1085, 1171, 2183, 2421
Bst6I CTCTTC 4 cut(s) 159, 2148, 2368, 2643
BstACI GRCGYC 1 cut(s) 2621
BstAPI GCANNNNNTGC 2 cut(s) 930, 2258
BstBAI YACGTR 2 cut(s) 2027, 2036
BstC8I GCNNGC 7 cut(s) 1138, 1556, 2018, 2111, 2305, 2515, 2705
BstDEI CTNAG 7 cut(s) 112, 729, 1043, 1209, 1707, 1935, 2187
BstDSI CCRYGG 3 cut(s) 1695, 2280, 2559
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 9 cut(s) 370, 382, 760, 913, 1410, 1423, 1752, 1761, 1770
BstFNI CGCG 6 cut(s) 847, 1330, 1995, 2109, 2133, 2282
BstHHI GCGC 2 cut(s) 439, 2428
BstMCI CGRYCG 2 cut(s) 2280, 2587
BstNI CCWGG 5 cut(s) 228, 348, 537, 648, 1563
BstNSI RCATGY 3 cut(s) 1471, 1558, 1639
BstSCI CCNGG 8 cut(s) 226, 346, 535, 646, 750, 1561, 1857, 2657
BstSFI CTRYAG 2 cut(s) 1167, 2247
BstSLI GKGCMC 2 cut(s) 1526, 2664
BstUI CGCG 6 cut(s) 847, 1330, 1995, 2109, 2133, 2282
BstX2I RGATCY 2 cut(s) 1489, 1714
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 2 cut(s) 1489, 1714
BstZI CGGCCG 1 cut(s) 2277
BsuI GTATCC 1 cut(s) 2404
BtgI CCRYGG 3 cut(s) 1695, 2280, 2559
BtgZI GCGATG 2 cut(s) 52, 1923
BtrI CACGTC 1 cut(s) 578
BtsCI GGATG 9 cut(s) 370, 382, 760, 913, 1410, 1423, 1752, 1761, 1770
BtsI GCAGTG 3 cut(s) 664, 877, 2373
BveI ACCTGC 1 cut(s) 2245
Cac8I GCNNGC 7 cut(s) 1138, 1556, 2018, 2111, 2305, 2515, 2705
CaiI CAGNNNCTG 2 cut(s) 1175, 2261
CciI TCATGA 1 cut(s) 1782
CciNI GCGGCCGC 1 cut(s) 2277
CfoI GCGC 2 cut(s) 439, 2428
Cfr42I CCGCGG 1 cut(s) 2283
CseI GACGC 3 cut(s) 1347, 2001, 2610
Csp6I GTAC 8 cut(s) 123, 224, 289, 1107, 1543, 2485, 2566, 2614
CviQI GTAC 8 cut(s) 123, 224, 289, 1107, 1543, 2485, 2566, 2614
DdeI CTNAG 7 cut(s) 112, 729, 1043, 1209, 1707, 1935, 2187
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 3 cut(s) 2199, 2277, 2502
EagI CGGCCG 1 cut(s) 2277
Eam1104I CTCTTC 4 cut(s) 159, 2148, 2368, 2643
EarI CTCTTC 4 cut(s) 159, 2148, 2368, 2643
EciI GGCGGA 4 cut(s) 1692, 2200, 2300, 2420
EclXI CGGCCG 1 cut(s) 2277
Eco130I CCWWGG 6 cut(s) 498, 514, 1695, 2460, 2505, 2559
Eco147I AGGCCT 2 cut(s) 235, 2360
Eco24I GRGCYC 2 cut(s) 1526, 2655
Eco47I GGWCC 8 cut(s) 518, 533, 1601, 1625, 1655, 1663, 1913, 2116
Eco52I CGGCCG 1 cut(s) 2277
Eco57I CTGAAG 2 cut(s) 417, 1341
Eco72I CACGTG 2 cut(s) 2027, 2036
Eco88I CYCGRG 3 cut(s) 869, 2330, 2665
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 4 cut(s) 518, 1523, 1663, 2019
EcoRI GAATTC 1 cut(s) 723
EcoRII CCWGG 5 cut(s) 226, 346, 535, 646, 1561
EcoT14I CCWWGG 6 cut(s) 498, 514, 1695, 2460, 2505, 2559
EcoT22I ATGCAT 1 cut(s) 1318
EcoT38I GRGCYC 2 cut(s) 1526, 2655
ErhI CCWWGG 6 cut(s) 498, 514, 1695, 2460, 2505, 2559
FalI AAGNNNNNCTT 6 cut(s) 156, 188, 917, 949, 1393, 1425
FaqI GGGAC 2 cut(s) 1611, 2129
FauI CCCGC 3 cut(s) 111, 1534, 2106
FauNDI CATATG 1 cut(s) 1594
FbaI TGATCA 1 cut(s) 2544
FblI GTMKAC 1 cut(s) 2498
FokI GGATG 9 cut(s) 377, 389, 767, 920, 1417, 1430, 1759, 1768, 1777
FriOI GRGCYC 2 cut(s) 1526, 2655
FspBI CTAG 1 cut(s) 309
GlaI GCGC 2 cut(s) 438, 2427
GsaI CCCAGC 2 cut(s) 1104, 1273
GsuI CTGGAG 2 cut(s) 477, 1992
HapII CCGG 5 cut(s) 431, 752, 1858, 2399, 2658
HgaI GACGC 3 cut(s) 1347, 2001, 2610
HhaI GCGC 2 cut(s) 439, 2428
Hin1I GRCGYC 1 cut(s) 2621
Hin6I GCGC 2 cut(s) 437, 2426
HinP1I GCGC 2 cut(s) 437, 2426
HincII GTYRAC 1 cut(s) 2499
HindII GTYRAC 1 cut(s) 2499
HindIII AAGCTT 1 cut(s) 2323
HpaII CCGG 5 cut(s) 431, 752, 1858, 2399, 2658
Hpy166II GTNNAC 6 cut(s) 224, 291, 836, 1145, 1658, 2499
Hpy188I TCNGA 9 cut(s) 262, 1212, 1243, 1321, 1605, 1710, 1809, 2083, 2188
Hpy8I GTNNAC 6 cut(s) 224, 291, 836, 1145, 1658, 2499
HpyCH4III ACNGT 5 cut(s) 409, 1085, 1171, 2183, 2421
HpyCH4IV ACGT 6 cut(s) 203, 577, 696, 1251, 2026, 2035
HpyF3I CTNAG 7 cut(s) 112, 729, 1043, 1209, 1707, 1935, 2187
HpySE526I ACGT 6 cut(s) 203, 577, 696, 1251, 2026, 2035
Hsp92I GRCGYC 1 cut(s) 2621
HspAI GCGC 2 cut(s) 437, 2426
Kpn2I TCCGGA 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 2544
KspI CCGCGG 1 cut(s) 2283
LguI GCTCTTC 1 cut(s) 2643
LmnI GCTCC 4 cut(s) 433, 2140, 2287, 2395
MaeI CTAG 1 cut(s) 309
MaeII ACGT 6 cut(s) 203, 577, 696, 1251, 2026, 2035
MaeIII GTNAC 9 cut(s) 142, 250, 1079, 1203, 1359, 1985, 2027, 2036, 2622
MbiI CCGCTC 3 cut(s) 2113, 2290, 2390
MflI RGATCY 2 cut(s) 1489, 1714
MhlI GDGCHC 5 cut(s) 1526, 1678, 2145, 2655, 2664
MluCI AATT 6 cut(s) 17, 213, 390, 688, 723, 1796
MluI ACGCGT 2 cut(s) 845, 1328
MlyI GAGTC 7 cut(s) 1079, 1139, 1343, 1767, 2198, 2453, 2579
Mph1103I ATGCAT 1 cut(s) 1318
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 2471
MseI TTAA 6 cut(s) 21, 212, 474, 482, 699, 936
MslI CAYNNNNRTG 4 cut(s) 740, 1571, 1634, 2148
MspA1I CMGCKG 5 cut(s) 1529, 1902, 2258, 2282, 2520
MspI CCGG 5 cut(s) 431, 752, 1858, 2399, 2658
MspR9I CCNGG 8 cut(s) 228, 348, 537, 648, 752, 1563, 1859, 2659
MvaI CCWGG 5 cut(s) 228, 348, 537, 648, 1563
MvnI CGCG 6 cut(s) 847, 1330, 1995, 2109, 2133, 2282
NciI CCSGG 3 cut(s) 752, 1859, 2659
NcoI CCATGG 2 cut(s) 1695, 2559
NdeI CATATG 1 cut(s) 1594
NmeAIII GCCGAG 2 cut(s) 2177, 2665
NmuCI GTSAC 6 cut(s) 142, 1359, 1985, 2027, 2036, 2622
NotI GCGGCCGC 1 cut(s) 2277
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 3 cut(s) 1471, 1558, 1639
PaeI GCATGC 1 cut(s) 1558
PaeR7I CTCGAG 1 cut(s) 2665
PagI TCATGA 1 cut(s) 1782
PceI AGGCCT 2 cut(s) 235, 2360
PciSI GCTCTTC 1 cut(s) 2643
PcsI WCGNNNNNNNCGW 5 cut(s) 138, 2032, 2453, 2462, 2574
PdmI GAANNNNTTC 1 cut(s) 2471
PfeI GAWTC 7 cut(s) 41, 101, 190, 257, 326, 1156, 1238
PflFI GACNNNGTC 2 cut(s) 137, 1848
PflMI CCANNNNNTGG 1 cut(s) 719
Ple19I CGATCG 1 cut(s) 2587
PleI GAGTC 7 cut(s) 1078, 1138, 1342, 1767, 2197, 2452, 2578
PmaCI CACGTG 2 cut(s) 2027, 2036
PmlI CACGTG 2 cut(s) 2027, 2036
PpsI GAGTC 7 cut(s) 1078, 1138, 1342, 1767, 2197, 2452, 2578
Ppu21I YACGTR 2 cut(s) 2027, 2036
PpuMI RGGWCCY 2 cut(s) 518, 1663
PsiI TTATAA 2 cut(s) 15, 1536
Psp5II RGGWCCY 2 cut(s) 518, 1663
Psp6I CCWGG 5 cut(s) 226, 346, 535, 646, 1561
PspCI CACGTG 2 cut(s) 2027, 2036
PspFI CCCAGC 2 cut(s) 1100, 1269
PspGI CCWGG 5 cut(s) 226, 346, 535, 646, 1561
PspOMI GGGCCC 1 cut(s) 1522
PspPPI RGGWCCY 2 cut(s) 518, 1663
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PstI CTGCAG 1 cut(s) 2251
PstNI CAGNNNCTG 2 cut(s) 1175, 2261
PsuI RGATCY 2 cut(s) 1489, 1714
PsyI GACNNNGTC 2 cut(s) 137, 1848
PvuI CGATCG 1 cut(s) 2587
PvuII CAGCTG 1 cut(s) 2258
RsaI GTAC 8 cut(s) 124, 225, 290, 1108, 1544, 2486, 2567, 2615
RsaNI GTAC 8 cut(s) 123, 224, 289, 1107, 1543, 2485, 2566, 2614
RseI CAYNNNNRTG 4 cut(s) 740, 1571, 1634, 2148
SacII CCGCGG 1 cut(s) 2283
SalI GTCGAC 1 cut(s) 2497
SapI GCTCTTC 1 cut(s) 2643
SaqAI TTAA 6 cut(s) 21, 212, 474, 482, 699, 936
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 7 cut(s) 1079, 1139, 1343, 1767, 2198, 2453, 2579
ScrFI CCNGG 8 cut(s) 228, 348, 537, 648, 752, 1563, 1859, 2659
SduI GDGCHC 5 cut(s) 1526, 1678, 2145, 2655, 2664
SfcI CTRYAG 2 cut(s) 1167, 2247
Sfr274I CTCGAG 1 cut(s) 2665
Sfr303I CCGCGG 1 cut(s) 2283
SgrBI CCGCGG 1 cut(s) 2283
SgrDI CGTCGACG 1 cut(s) 2497
SinI GGWCC 8 cut(s) 518, 533, 1601, 1625, 1655, 1663, 1913, 2116
SlaI CTCGAG 1 cut(s) 2665
SmiMI CAYNNNNRTG 4 cut(s) 740, 1571, 1634, 2148
SmlI CTYRAG 4 cut(s) 1485, 1677, 2042, 2665
SmoI CTYRAG 4 cut(s) 1485, 1677, 2042, 2665
SphI GCATGC 1 cut(s) 1558
Sse9I AATT 6 cut(s) 17, 213, 390, 688, 723, 1796
SseBI AGGCCT 2 cut(s) 235, 2360
SspMI CTAG 1 cut(s) 309
StuI AGGCCT 2 cut(s) 235, 2360
StyD4I CCNGG 8 cut(s) 226, 346, 535, 646, 750, 1561, 1857, 2657
StyI CCWWGG 6 cut(s) 498, 514, 1695, 2460, 2505, 2559
TaaI ACNGT 5 cut(s) 409, 1085, 1171, 2183, 2421
TaiI ACGT 6 cut(s) 206, 580, 699, 1254, 2029, 2038
TaqI TCGA 6 cut(s) 486, 2125, 2498, 2573, 2609, 2666
TaqII GACCGA 1 cut(s) 2133
TasI AATT 6 cut(s) 17, 213, 390, 688, 723, 1796
TatI WGTACW 3 cut(s) 288, 1106, 2484
TfiI GAWTC 7 cut(s) 41, 101, 190, 257, 326, 1156, 1238
Tru1I TTAA 6 cut(s) 21, 212, 474, 482, 699, 936
Tru9I TTAA 6 cut(s) 21, 212, 474, 482, 699, 936
TseFI GTSAC 6 cut(s) 142, 1359, 1985, 2027, 2036, 2622
Tsp45I GTSAC 6 cut(s) 142, 1359, 1985, 2027, 2036, 2622
TspGWI ACGGA 2 cut(s) 1439, 1530
Tth111I GACNNNGTC 2 cut(s) 137, 1848
Van91I CCANNNNNTGG 1 cut(s) 719
VpaK11BI GGWCC 8 cut(s) 518, 533, 1601, 1625, 1655, 1663, 1913, 2116
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 5 cut(s) 17, 390, 688, 723, 1796
XceI RCATGY 3 cut(s) 1471, 1558, 1639
XcmI CCANNNNNNNNNTGG 2 cut(s) 501, 521
XhoI CTCGAG 1 cut(s) 2665
XmiI GTMKAC 1 cut(s) 2498
XmnI GAANNNNTTC 1 cut(s) 2471
XspI CTAG 1 cut(s) 309
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.