pycom06g04660

DWNN domain, A CCHC-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001377
Physical Location & Seq
Forward (+)
6068876 .. 6078293
9418 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g04660.1

Sequence Viewer

Length: 3657 bp
ATGGCGGTCTATTATAAATTTAAGAGTGCAAAAGATTATGATTCTATTCCTATGGACGGCCCGTTCATCGCAGTTGGTATTTTGAAAGAAAAAATATATGAATCCAAGCACTTAGGGCGGGGTACTGATTACGACCTCGTCGTCACCAATGCCCAGACCAATGAAGAGTATCTTGATGAAGCGATGTTGATTCCAAAAAATACGTCAGTTTTAATTCGCCGTGTACCTGGAAGGCCTCCAATGCCTATTGTTACTGATTCAGAGCGAAAGGTGGAAGATAAGGTGGACACTGAACCAGAAAGGACTAGCTTTCCGGCGGCTGAATCATCTGCCACGAAATATCCTGGTGATCCAGATTGGGATGATTATGGGGATGATTTGTATAAAATTCCTGAAGTGATGACAGTGCAGACAAGTTATCAGGCTATGGATGCGCAGCCAACTGATAAAGCTGATGAAGATAGCAAGATTAAGGCTTTAATCGACACTCCAGCCTTGGAATGGCAACACCAAGGTCCTGATGGCTTTGGTCCTGGTAGAGGTTTTGGCAGGGGTGGAAGAATGATGAATGGACGTGGTATTGGTCGAGGAGTAGGGGGCTTCGAGAGGAAAACACCTCCACAGGGCTATATTTGTCACAGGTGTAAGGTGCCTGGGCATTATATTCAGCACTGCCCTACGAATGGTGATCCAAATTTTGACATAAAAAGAGTGAAGCCACCCACTGGAATTCCTAAGTCCATGCTGATGGCAAACCCGGATGGCTCATATAAGTTGCCAAGTGGTGATAAAGCTGTATTGAGGCCGAATGAGGCTGCTTTTGAGAAAGAGATTGAAGGTTTACCATCTACGCGTTCTGTTGGTGATCTACCGCCCGAGCTACACTGCCCGTTGTGCAAAGAAGTGATGAAAGATGCTGTGCTAACGAGCAAGTGTTGCTTTAATAGCTTTTGTGATAAGTGTATCAGGAACTACATTATGTCCAAGTCAGTGTGTGTCTGTGGGGCTACAAATACACTGGCAGATGATCTACTGCCAAATAAGACACTGAGGGATACAATCAATCGCATCTTGGAGTCTGGTGGTAACAGTAGTGCTGACAATGCTGGGAGTACTTTCCAAGTTCAAGATATGGAGTCTGCTCGCTGTCCACAACCCAAGATTCCTTCCCCTACAGTATCTGCTGCATCAAAAGGAGATGAAAAGCAGTTACCTCAGAATGAAGAACCTCCAAAGTTTCCTGAATCTGAAAATGACGTAAAGCCTGTTCTTCCCCAGCAGCAGATTTTAGAGAGAGTGAGAACTACAAAAGTAGCTGATGCATCTGAAGCCACGCGTGAGTCAATGAGTGTGAAGGAACCAGCGTCACAAGGTAGTGTTCCACTGGTTGAGGAGGAAGTGCAGCAAAGGATGGCTTCTGGGGAGGCAGGAAAGAAAAGGAAAAAGAAGAAAATTCGTGTGCCTGTGAATGCCGAAAACTATATGATGCCTATGGGCCCCGCTGCTTATAATCCATACTGGAATGGCATGCAACCTGGCATGGGCATGGAAGGATATATGACACCATATGGTGGTCCGATGCCGTATATGGGTTATGGTCCCATTGACATGCCATTTGGGGGTCTTGGTCCACAGGACCCATTTGGTGCTCAAGGTTATATGATGCCCATGGTTCCGCCTCAGAGGGATCTTGCGGACTTTGGTATGGGCATGAATGGCATGAATGGGATGAATGGGATGAATGGACCCCCCATCATGAGCAAAGAAGAATTTGAGGCTCGGAAAGCTGATTTGAGAAGGAAGCGTGAAAATGAAAGACAGGGTCAAAGCCGGGAGTTCTCCAAAGACAGGGAATACAGAAGGGAAGTTAGCAGCGGTGGCGATGGTCCTCCAATGAAATCCAAATCTAAGTCAATGGCCCGATCACCAAGCCCAGACTATCCTAATCAGCACCGCCGTCACCGACACGAGAGGCAATCTCCAGAACGCAGGCCCTCACGTGACCCACGTGACCTGGAGCCACAGCCGCCTCCACGTCCTCCAAAGAGAAAATCCGAGCAGCACCACCACGACAGGGAACGCGAGCGGGACCGAGATCGAGAACGCGAGAGGGAGCACGACCACCACGACCATGAGGAGGATCATCCCCGCCAGAAACGACACCGTTCTGAGTCGTCCTCAGCCAAACCTTCTGCCCAAACGACAGCATCGGCGAAACCTGCCTCCTCTGCAGCATCAGCTGCTGCGGCAGAAGCGGCGGCCGCGGCGGAGCGGAAGCACAAGGCAAGCGTGTTCTCTCGCATAAGTTTTCCCGAGGGAGAAGTGAGCAAGAAGCGGAAGGCCTCTTCATCCTCCACTGCTTCTCCGGCGGCGGAGGATACCAAACCGTCGGCGCATCATCATCACAAGGAGTCGTCGTCGTCCTTGGCGAATGGGTTCTACGACGAGTACAAGTCGTCGTCGACGGCCAAGGCTGCGAGCAGCGGAACTGGAAGCAGAAAGACTATGATCAGTGCTGCTTCCATGGAGTACGAGTCGAGCGACGATCGATCGGCACTTCAAGAGGAAGCCGTCGAGGTACGAGGCTTCGCCGCCGCAGGCCGCCGAGCAGGAAGAGCCCTCCAGGCACTCGAGAGGAGGCAGGGATCGGGATCGCAGGCAGAGATAGTGTTTTGGGTAACGCCGAAGTTGGCTTTCTCCGAGAACGACCTCGTTCGGCCCCACTCACTTAATGTATTGGTGTTGGGCCTTTTGGGGCGCTGGGTGTTGTACTGTATTTGTAAAGAAGCCCAATCGGGAGTTGTTTCGGGGGCCGAATGTATGTTTTCGTGTTTTTTGTTGATGCTTGATATTTCGTATCCGATGTGTTTTGCGTATCTTAAAAACCCAAAAATTACAAATATAAATAAATTTACTGATGGAAATCCCAATCCTTTCCAGCACTATAAAGTCGAGTTTTCACCGCAAAGCCTCACTGTGTTTGGAAAAACCCTAGCCCCGTCTGCCGAACCCTGCGCGTTGCAGCTCGTTTCTGATCCGACCATGGGAGCTTACAAGTACGTATCCGAGATATGGAGGAAGAAGCAGTCCGATGTGATGAGATTCCTCCAGAGGGTCCGCTGCTGGGAGTATCGCCAACATCCCTCGATTGTCCGAGTCACAAGACCCACACGCCCTGATAAGGCGCGTCGTTTAGGTTACAAGGCCAAGCAGGGTTATGTTGTTTACCGTGTCCGTGTGAGGCGTGGTGGCCGCAAGAGGCCTGTTTCCAAGGGTATTGTATATGGAAAGCCCACAAACCAGGGTGTGACACAGCTCAAGTTCCAGCGTAGCAAGAGGTCTGTTGCTGAGGAGCGTGCAGGGCGCAAGTTGGGAGGTCTCAGGGTTCTCAACTCATACTGGCTCAATGAGGACTCGACCTACAAGTACTTTGAAGTTATCTTGGTTGATGTTGCCCACAATGCCATCAGGAATGACCCAAGAATCAACTGGCTTTGCAACCCTGTTCATAAGCACAGAGAGCTTCGTGGTCTTACTTCTGCTGGAAAGAAATACAGGGGTCTTCGTGGAAAGGGACATCTGTACCACAAGAACCGACCTTCTCGCAGAGCAACCTGGAAGAGAAACACCACCCTTTCACTCCGTCGTTACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005815 GO:0005829 GO:0005856 GO:0006275 GO:0006325 GO:0006464 GO:0006479 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0006979 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009056 GO:0009057 GO:0009414 GO:0009415 GO:0009628 GO:0009635 GO:0009636 GO:0009651 GO:0009743 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010555 GO:0010556 GO:0015630 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016607 GO:0016740 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032991 GO:0033554 GO:0034969 GO:0034971 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046677 GO:0046686 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051276 GO:0051603 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0072756 GO:0080090 GO:0080134 GO:0097237 GO:0140096 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902882 GO:1902883 GO:1902884 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DWNN zf-CCHC_2

Protein Analysis

1219

Amino Acids

136.34

Weight (kDa)

9.45

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DWNN PF08783 3 - 76 7.9e-30 DWNN domain
zf-CCHC_2 PF13696 207 - 227 7.1e-09 Zinc knuckle
Ribosomal_L15e PF00827 1016 - 1204 8.5e-90 Ribosomal L15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 1509
AasI GACNNNNNNGTC 1 cut(s) 140
Acc16I TGCGCA 1 cut(s) 435
Acc36I ACCTGC 1 cut(s) 2227
AccB1I GGYRCC 1 cut(s) 649
AccB7I CCANNNNNTGG 2 cut(s) 725, 1571
AccBSI CCGCTC 2 cut(s) 2086, 2272
AccI GTMKAC 1 cut(s) 2462
AccII CGCG 7 cut(s) 853, 1336, 2082, 2106, 2264, 3017, 3187
AclWI GGATC 7 cut(s) 344, 683, 1695, 2148, 2653, 2659, 3029
AcoI YGGCCR 3 cut(s) 2259, 2466, 3250
AcsI RAATTY 7 cut(s) 17, 387, 694, 729, 1452, 1769, 2909
AcuI CTGAAG 2 cut(s) 414, 1347
AcvI CACGTG 2 cut(s) 2000, 2009
AflIII ACRYGT 2 cut(s) 851, 1334
AgsI TTSAA 5 cut(s) 85, 836, 1127, 2561, 3434
AjiI CACGTC 2 cut(s) 575, 2036
AjnI CCWGG 9 cut(s) 226, 343, 532, 652, 1534, 2013, 2622, 3300, 3614
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
Alw21I GWGCWC 2 cut(s) 1651, 2118
Alw26I GTCTC 1 cut(s) 3383
AlwI GGATC 7 cut(s) 344, 683, 1695, 2148, 2653, 2659, 3029
AlwNI CAGNNNCTG 2 cut(s) 1181, 2243
Ama87I CYCGRG 3 cut(s) 875, 2312, 2630
ApaI GGGCCC 1 cut(s) 1499
ApoI RAATTY 7 cut(s) 17, 387, 694, 729, 1452, 1769, 2909
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 1598, 1630
Asp700I GAANNNNTTC 1 cut(s) 2435
AspLEI GCGC 6 cut(s) 436, 2395, 2760, 3017, 3187, 3366
AsuC2I CCSGG 2 cut(s) 758, 1832
AsuHPI GGTGA 8 cut(s) 136, 359, 698, 797, 875, 1917, 1952, 2952
AvaI CYCGRG 3 cut(s) 875, 2312, 2630
BaeGI GKGCMC 1 cut(s) 1499
BaeI ACNNNNGTAYC 2 cut(s) 3566, 3599
BanI GGYRCC 1 cut(s) 649
BanII GRGCYC 2 cut(s) 1499, 2620
BauI CACGAG 1 cut(s) 1967
BbrPI CACGTG 2 cut(s) 2000, 2009
BbsI GAAGAC 1 cut(s) 3554
Bbv12I GWGCWC 2 cut(s) 1651, 2118
BbvCI CCTCAGC 2 cut(s) 2179, 3348
BccI CCATC 9 cut(s) 515, 742, 755, 853, 1405, 1760, 1877, 2912, 3473
BceAI ACGGC 6 cut(s) 73, 204, 1567, 1941, 2481, 2555
BciT130I CCWGG 9 cut(s) 228, 345, 534, 654, 1536, 2015, 2624, 3302, 3616
BciVI GTATCC 4 cut(s) 1048, 2371, 2868, 3073
BclI TGATCA 1 cut(s) 2508
BcnI CCSGG 2 cut(s) 758, 1832
BcoDI GTCTC 1 cut(s) 3383
BfaI CTAG 2 cut(s) 306, 2993
BfmI CTRYAG 2 cut(s) 1173, 2229
BfoI RGCGCY 1 cut(s) 2761
BfuAI ACCTGC 1 cut(s) 2227
BfuI GTATCC 4 cut(s) 1048, 2371, 2868, 3073
BglI GCCNNNNNGGC 1 cut(s) 2624
BmcAI AGTACT 2 cut(s) 1114, 3428
BmeT110I CYCGRG 3 cut(s) 875, 2312, 2630
BmgBI CACGTC 2 cut(s) 575, 2036
BpiI GAAGAC 1 cut(s) 3554
BplI GAGNNNNNCTC 4 cut(s) 1963, 1995, 2162, 2194
BpmI CTGGAG 5 cut(s) 474, 1965, 2036, 2606, 3092
Bpu10I CCTNAGC 2 cut(s) 2179, 3348
BpuEI CTTGAG 2 cut(s) 1635, 3302
BpuMI CCSGG 2 cut(s) 758, 1832
Bsa29I ATCGAT 1 cut(s) 2548
BsaAI YACGTR 3 cut(s) 2000, 2009, 3061
BsaBI GATNNNNATC 2 cut(s) 2650, 2922
BsaI GGTCTC 1 cut(s) 3383
BsaXI ACNNNNNCTCC 6 cut(s) 2347, 2377, 2402, 2432, 2519, 2549
Bse1I ACTGG 7 cut(s) 730, 1023, 1389, 1523, 2494, 3404, 3494
Bse8I GATNNNNATC 2 cut(s) 2650, 2922
BseBI CCWGG 9 cut(s) 228, 345, 534, 654, 1536, 2015, 2624, 3302, 3616
BseCI ATCGAT 1 cut(s) 2548
BseJI GATNNNNATC 2 cut(s) 2650, 2922
BseMII CTCAG 7 cut(s) 1040, 1229, 1694, 2160, 2193, 3339, 3394
BseNI ACTGG 7 cut(s) 730, 1023, 1389, 1523, 2494, 3404, 3494
BseRI GAGGAG 6 cut(s) 603, 1406, 2150, 2215, 2650, 3365
BseSI GKGCMC 1 cut(s) 1499
BseX3I CGGCCG 1 cut(s) 2259
BseYI CCCAGC 4 cut(s) 1106, 1275, 2760, 3123
BsgI GTGCAG 3 cut(s) 428, 1421, 3378
Bsh1236I CGCG 7 cut(s) 853, 1336, 2082, 2106, 2264, 3017, 3187
Bsh1285I CGRYCG 3 cut(s) 2262, 2548, 2552
BshNI GGYRCC 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 2548
BsiEI CGRYCG 3 cut(s) 2262, 2548, 2552
BsiHKAI GWGCWC 2 cut(s) 1651, 2118
BsiHKCI CYCGRG 3 cut(s) 875, 2312, 2630
BsiSI CCGG 4 cut(s) 314, 758, 1831, 2366
BslFI GGGAC 3 cut(s) 1584, 2102, 3588
BsmAI GTCTC 1 cut(s) 3383
BsmFI GGGAC 3 cut(s) 1584, 2102, 3588
BsmI GAATGC 1 cut(s) 1474
Bso31I GGTCTC 1 cut(s) 3383
BsoBI CYCGRG 3 cut(s) 875, 2312, 2630
Bsp120I GGGCCC 1 cut(s) 1495
Bsp1286I GDGCHC 4 cut(s) 1499, 1651, 2118, 2620
Bsp19I CCATGG 3 cut(s) 1668, 2523, 3042
BspCNI CTCAG 7 cut(s) 1041, 1228, 1693, 2161, 2192, 3340, 3393
BspDI ATCGAT 1 cut(s) 2548
BspFNI CGCG 7 cut(s) 853, 1336, 2082, 2106, 2264, 3017, 3187
BspHI TCATGA 1 cut(s) 1755
BspMAI CTGCAG 1 cut(s) 2233
BspMI ACCTGC 1 cut(s) 2227
BspPI GGATC 7 cut(s) 344, 683, 1695, 2148, 2653, 2659, 3029
BspQI GCTCTTC 1 cut(s) 2608
BspT107I GGYRCC 1 cut(s) 649
BspTNI GGTCTC 1 cut(s) 3383
BsrBI CCGCTC 2 cut(s) 2086, 2272
BsrI ACTGG 7 cut(s) 730, 1023, 1389, 1523, 2494, 3404, 3494
BssSI CACGAG 1 cut(s) 1967
BssT1I CCWWGG 8 cut(s) 495, 511, 1668, 2424, 2469, 2523, 3042, 3270
Bst2BI CACGAG 1 cut(s) 1967
Bst2UI CCWGG 9 cut(s) 228, 345, 534, 654, 1536, 2015, 2624, 3302, 3616
Bst4CI ACNGT 9 cut(s) 406, 1091, 1177, 2165, 2388, 2774, 2977, 3230, 3653
Bst6I CTCTTC 4 cut(s) 159, 2350, 2608, 3614
BstAPI GCANNNNNTGC 2 cut(s) 936, 2240
BstBAI YACGTR 3 cut(s) 2000, 2009, 3061
BstC8I GCNNGC 9 cut(s) 1144, 1529, 1991, 2084, 2287, 2479, 2599, 2658, 3357
BstDSI CCRYGG 4 cut(s) 1668, 2262, 2523, 3042
BstENI CCTNNNNNAGG 1 cut(s) 537
BstFNI CGCG 7 cut(s) 853, 1336, 2082, 2106, 2264, 3017, 3187
BstH2I RGCGCY 1 cut(s) 2761
BstHHI GCGC 6 cut(s) 436, 2395, 2760, 3017, 3187, 3366
BstMAI GTCTC 1 cut(s) 3383
BstMCI CGRYCG 3 cut(s) 2262, 2548, 2552
BstNI CCWGG 9 cut(s) 228, 345, 534, 654, 1536, 2015, 2624, 3302, 3616
BstNSI RCATGY 2 cut(s) 1531, 1612
BstSFI CTRYAG 2 cut(s) 1173, 2229
BstSLI GKGCMC 1 cut(s) 1499
BstSNI TACGTA 1 cut(s) 3061
BstUI CGCG 7 cut(s) 853, 1336, 2082, 2106, 2264, 3017, 3187
BstV2I GAAGAC 1 cut(s) 3554
BstX2I RGATCY 1 cut(s) 1687
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 1 cut(s) 1687
BstZI CGGCCG 1 cut(s) 2259
Bsu15I ATCGAT 1 cut(s) 2548
BsuI GTATCC 4 cut(s) 1048, 2371, 2868, 3073
BsuTUI ATCGAT 1 cut(s) 2548
BtgI CCRYGG 4 cut(s) 1668, 2262, 2523, 3042
BtgZI GCGATG 3 cut(s) 52, 197, 1896
BtrI CACGTC 2 cut(s) 575, 2036
BtsI GCAGTG 3 cut(s) 670, 883, 2355
BveI ACCTGC 1 cut(s) 2227
Cac8I GCNNGC 9 cut(s) 1144, 1529, 1991, 2084, 2287, 2479, 2599, 2658, 3357
CaiI CAGNNNCTG 2 cut(s) 1181, 2243
CciI TCATGA 1 cut(s) 1755
CciNI GCGGCCGC 1 cut(s) 2259
CfoI GCGC 6 cut(s) 436, 2395, 2760, 3017, 3187, 3366
Cfr42I CCGCGG 1 cut(s) 2265
ClaI ATCGAT 1 cut(s) 2548
CseI GACGC 2 cut(s) 1353, 3176
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 3 cut(s) 2259, 2466, 3250
EagI CGGCCG 1 cut(s) 2259
Eam1104I CTCTTC 4 cut(s) 159, 2350, 2608, 3614
EarI CTCTTC 4 cut(s) 159, 2350, 2608, 3614
EciI GGCGGA 3 cut(s) 1665, 2282, 2387
EclXI CGGCCG 1 cut(s) 2259
Eco105I TACGTA 1 cut(s) 3061
Eco130I CCWWGG 8 cut(s) 495, 511, 1668, 2424, 2469, 2523, 3042, 3270
Eco147I AGGCCT 3 cut(s) 235, 2342, 3262
Eco24I GRGCYC 2 cut(s) 1499, 2620
Eco31I GGTCTC 1 cut(s) 3383
Eco52I CGGCCG 1 cut(s) 2259
Eco57I CTGAAG 2 cut(s) 414, 1347
Eco72I CACGTG 2 cut(s) 2000, 2009
Eco88I CYCGRG 3 cut(s) 875, 2312, 2630
EcoNI CCTNNNNNAGG 1 cut(s) 537
EcoO109I RGGNCCY 4 cut(s) 515, 1496, 1636, 1992
EcoRI GAATTC 1 cut(s) 729
EcoRII CCWGG 9 cut(s) 226, 343, 532, 652, 1534, 2013, 2622, 3300, 3614
EcoT14I CCWWGG 8 cut(s) 495, 511, 1668, 2424, 2469, 2523, 3042, 3270
EcoT22I ATGCAT 1 cut(s) 1324
EcoT38I GRGCYC 2 cut(s) 1499, 2620
ErhI CCWWGG 8 cut(s) 495, 511, 1668, 2424, 2469, 2523, 3042, 3270
FalI AAGNNNNNCTT 6 cut(s) 156, 188, 923, 955, 1399, 1431
FaqI GGGAC 3 cut(s) 1584, 2102, 3588
FauI CCCGC 4 cut(s) 111, 1507, 2079, 2156
FauNDI CATATG 1 cut(s) 1567
FbaI TGATCA 1 cut(s) 2508
FblI GTMKAC 1 cut(s) 2462
FriOI GRGCYC 2 cut(s) 1499, 2620
FspBI CTAG 2 cut(s) 306, 2993
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 6 cut(s) 435, 2394, 2759, 3016, 3186, 3365
GsaI CCCAGC 4 cut(s) 1110, 1279, 2764, 3127
GsuI CTGGAG 5 cut(s) 474, 1965, 2036, 2606, 3092
HaeII RGCGCY 1 cut(s) 2761
HapII CCGG 4 cut(s) 314, 758, 1831, 2366
HgaI GACGC 2 cut(s) 1353, 3176
HhaI GCGC 6 cut(s) 436, 2395, 2760, 3017, 3187, 3366
Hin6I GCGC 6 cut(s) 434, 2393, 2758, 3015, 3185, 3364
HinP1I GCGC 6 cut(s) 434, 2393, 2758, 3015, 3185, 3364
HincII GTYRAC 1 cut(s) 2463
HindII GTYRAC 1 cut(s) 2463
HpaII CCGG 4 cut(s) 314, 758, 1831, 2366
HphI GGTGA 8 cut(s) 136, 359, 698, 797, 875, 1917, 1952, 2952
Hpy166II GTNNAC 7 cut(s) 224, 286, 842, 1151, 1631, 2463, 3226
Hpy8I GTNNAC 7 cut(s) 224, 286, 842, 1151, 1631, 2463, 3226
HpyCH4III ACNGT 9 cut(s) 406, 1091, 1177, 2165, 2388, 2774, 2977, 3230, 3653
HpyCH4IV ACGT 7 cut(s) 203, 574, 1257, 1999, 2008, 2035, 3060
HpySE526I ACGT 7 cut(s) 203, 574, 1257, 1999, 2008, 2035, 3060
HspAI GCGC 6 cut(s) 434, 2393, 2758, 3015, 3185, 3364
Ksp22I TGATCA 1 cut(s) 2508
KspI CCGCGG 1 cut(s) 2265
LguI GCTCTTC 1 cut(s) 2608
LmnI GCTCC 5 cut(s) 2017, 2113, 2269, 3047, 3352
MaeI CTAG 2 cut(s) 306, 2993
MaeII ACGT 7 cut(s) 203, 574, 1257, 1999, 2008, 2035, 3060
MbiI CCGCTC 2 cut(s) 2086, 2272
MflI RGATCY 1 cut(s) 1687
MhlI GDGCHC 4 cut(s) 1499, 1651, 2118, 2620
MluCI AATT 9 cut(s) 17, 213, 387, 694, 729, 1452, 1769, 2892, 2909
MluI ACGCGT 2 cut(s) 851, 1334
MlyI GAGTC 8 cut(s) 1085, 1145, 1349, 2180, 2420, 2543, 3165, 3407
MmeI TCCRAC 1 cut(s) 3062
Mph1103I ATGCAT 1 cut(s) 1324
MroXI GAANNNNTTC 1 cut(s) 2435
MseI TTAA 7 cut(s) 21, 212, 471, 479, 942, 2730, 2880
MslI CAYNNNNRTG 4 cut(s) 746, 1544, 1607, 2130
MspA1I CMGCKG 6 cut(s) 1502, 1875, 2240, 2264, 2484, 3120
MspI CCGG 4 cut(s) 314, 758, 1831, 2366
Mva1269I GAATGC 1 cut(s) 1474
MvaI CCWGG 9 cut(s) 228, 345, 534, 654, 1536, 2015, 2624, 3302, 3616
MvnI CGCG 7 cut(s) 853, 1336, 2082, 2106, 2264, 3017, 3187
NciI CCSGG 2 cut(s) 758, 1832
NcoI CCATGG 3 cut(s) 1668, 2523, 3042
NdeI CATATG 1 cut(s) 1567
NmeAIII GCCGAG 1 cut(s) 2630
NmuCI GTSAC 8 cut(s) 142, 635, 1365, 1958, 2000, 2009, 3157, 3307
NotI GCGGCCGC 1 cut(s) 2259
NsbI TGCGCA 1 cut(s) 435
NsiI ATGCAT 1 cut(s) 1324
NspI RCATGY 2 cut(s) 1531, 1612
PaeI GCATGC 1 cut(s) 1531
PaeR7I CTCGAG 1 cut(s) 2630
PagI TCATGA 1 cut(s) 1755
PceI AGGCCT 3 cut(s) 235, 2342, 3262
PciSI GCTCTTC 1 cut(s) 2608
PcsI WCGNNNNNNNCGW 5 cut(s) 138, 2005, 2124, 2426, 2538
PctI GAATGC 1 cut(s) 1474
PdmI GAANNNNTTC 1 cut(s) 2435
PfeI GAWTC 9 cut(s) 41, 101, 190, 257, 323, 1162, 1244, 3102, 3483
PflFI GACNNNGTC 2 cut(s) 137, 1821
PflMI CCANNNNNTGG 2 cut(s) 725, 1571
Ple19I CGATCG 2 cut(s) 2548, 2552
PleI GAGTC 8 cut(s) 1084, 1144, 1348, 2179, 2419, 2542, 3164, 3407
PmaCI CACGTG 2 cut(s) 2000, 2009
PmlI CACGTG 2 cut(s) 2000, 2009
PpsI GAGTC 8 cut(s) 1084, 1144, 1348, 2179, 2419, 2542, 3164, 3407
Ppu21I YACGTR 3 cut(s) 2000, 2009, 3061
PpuMI RGGWCCY 2 cut(s) 515, 1636
PsiI TTATAA 2 cut(s) 15, 1509
Psp5II RGGWCCY 2 cut(s) 515, 1636
Psp6I CCWGG 9 cut(s) 226, 343, 532, 652, 1534, 2013, 2622, 3300, 3614
PspCI CACGTG 2 cut(s) 2000, 2009
PspFI CCCAGC 4 cut(s) 1106, 1275, 2760, 3123
PspGI CCWGG 9 cut(s) 226, 343, 532, 652, 1534, 2013, 2622, 3300, 3614
PspOMI GGGCCC 1 cut(s) 1495
PspPPI RGGWCCY 2 cut(s) 515, 1636
PsrI GAACNNNNNNTAC 2 cut(s) 1251, 1283
PstI CTGCAG 1 cut(s) 2233
PstNI CAGNNNCTG 2 cut(s) 1181, 2243
PsuI RGATCY 1 cut(s) 1687
PsyI GACNNNGTC 2 cut(s) 137, 1821
PvuI CGATCG 2 cut(s) 2548, 2552
PvuII CAGCTG 1 cut(s) 2240
RseI CAYNNNNRTG 4 cut(s) 746, 1544, 1607, 2130
SacII CCGCGG 1 cut(s) 2265
SalI GTCGAC 1 cut(s) 2461
SapI GCTCTTC 1 cut(s) 2608
SaqAI TTAA 7 cut(s) 21, 212, 471, 479, 942, 2730, 2880
ScaI AGTACT 2 cut(s) 1114, 3428
SchI GAGTC 8 cut(s) 1085, 1145, 1349, 2180, 2420, 2543, 3165, 3407
SduI GDGCHC 4 cut(s) 1499, 1651, 2118, 2620
SfcI CTRYAG 2 cut(s) 1173, 2229
Sfr274I CTCGAG 1 cut(s) 2630
Sfr303I CCGCGG 1 cut(s) 2265
SgrBI CCGCGG 1 cut(s) 2265
SgrDI CGTCGACG 1 cut(s) 2461
SlaI CTCGAG 1 cut(s) 2630
SmiMI CAYNNNNRTG 4 cut(s) 746, 1544, 1607, 2130
SmlI CTYRAG 3 cut(s) 1650, 2630, 3317
SmoI CTYRAG 3 cut(s) 1650, 2630, 3317
SnaBI TACGTA 1 cut(s) 3061
SphI GCATGC 1 cut(s) 1531
Sse9I AATT 9 cut(s) 17, 213, 387, 694, 729, 1452, 1769, 2892, 2909
SseBI AGGCCT 3 cut(s) 235, 2342, 3262
SspMI CTAG 2 cut(s) 306, 2993
StuI AGGCCT 3 cut(s) 235, 2342, 3262
StyI CCWWGG 8 cut(s) 495, 511, 1668, 2424, 2469, 2523, 3042, 3270
TaaI ACNGT 9 cut(s) 406, 1091, 1177, 2165, 2388, 2774, 2977, 3230, 3653
TaiI ACGT 7 cut(s) 206, 577, 1260, 2002, 2011, 2038, 3063
TaqII GACCGA 1 cut(s) 2106
TasI AATT 9 cut(s) 17, 213, 387, 694, 729, 1452, 1769, 2892, 2909
TatI WGTACW 4 cut(s) 1112, 2448, 2769, 3426
TfiI GAWTC 9 cut(s) 41, 101, 190, 257, 323, 1162, 1244, 3102, 3483
Tru1I TTAA 7 cut(s) 21, 212, 471, 479, 942, 2730, 2880
Tru9I TTAA 7 cut(s) 21, 212, 471, 479, 942, 2730, 2880
TseFI GTSAC 8 cut(s) 142, 635, 1365, 1958, 2000, 2009, 3157, 3307
Tsp45I GTSAC 8 cut(s) 142, 635, 1365, 1958, 2000, 2009, 3157, 3307
TspGWI ACGGA 2 cut(s) 3224, 3632
Tth111I GACNNNGTC 2 cut(s) 137, 1821
Van91I CCANNNNNTGG 2 cut(s) 725, 1571
XagI CCTNNNNNAGG 1 cut(s) 537
XapI RAATTY 7 cut(s) 17, 387, 694, 729, 1452, 1769, 2909
XceI RCATGY 2 cut(s) 1531, 1612
XcmI CCANNNNNNNNNTGG 3 cut(s) 498, 518, 3486
XhoI CTCGAG 1 cut(s) 2630
XmiI GTMKAC 1 cut(s) 2462
XmnI GAANNNNTTC 1 cut(s) 2435
XspI CTAG 2 cut(s) 306, 2993
ZrmI AGTACT 2 cut(s) 1114, 3428
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.