pycom04g05550

DWNN domain, A CCHC-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001377
Physical Location & Seq
Forward (+)
5356186 .. 5362423
6238 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05550.8

Sequence Viewer

Length: 2775 bp
ATGGCGGTGTATTATAAATTTAAGAGTGCAAAAGACTATGATTCTATTCCTATGGACGGCCCGTTCATCGCAGTTGGTATTTTGAAAGAAAAAATATATGAATCCAAGCACTTAGGGCGGGGTACTGATTACGACCTCGTCGTCACCAATGCCCAGACCAATGAAGAGTATCTTGATGAAGGGATGTTGATTCCAAAAAATACATCAGTTTTGATACGCCGTGTACCTGGAAGGCCTCCGATGCCCATTGTTACTGATTCAGAGCGAAAGGTAGAAGATAAGGTGGAGGACACTGAACCAGAAAGGACTAGCTTTCTGGCAGCTGAATCATCTGCCACGAAATATCCTGGTGATCCAGATTGGGATGATTATGGGGATGATTTGTATGAAATTCCTGAAGTGCTGGCTGTGCAGACAAGCTATCAGGCTCCGGATGCGCAGCCAACTGATAAAGCTGATGAAGATAGCAAGATTAAGGCTTTGATTGACACTTCAGCCTTGGAATGGCAACACCAAGGTCCCGATGGCTTTGGTCCTGGTAGAGGTTTTGGCAGGGGTGGAAGAATGATGAATGGACGTGGTATTGGTCGCGGAGGAGGGGGTTTTGAGAGGAAAACACCTCCACACGGATATATTTGTCATAGGTGTAAGGTGCCTGGGCATTATATTCAGCACTGCCCTACGAATGGTGATCGAAATTTCGACATTAAAAGAGTTAAGCCACCCACTGGAATTCCTAAGTCCATGCTGATGGCAAACCCAGATGGCTCATATAAATTGCCAAGTGGTGATAAAGCTGTATTGAGGCCGAACGAGGCTGCTTTTGAGAAAGAGATTGAAGGTTTACCATCTACACGTTCTGTTGGTGATCTACCGCCTGAGCTACACTGCCCATTGTGCAAAGAAGTGATGAAAGATGCTGTGCTAACGAGCAAGTGCTGCTTTAAAAGCTTTTGTGATAAGTGTATCCGGAACTACATTATGTCCATGTCAGTGTGTGTCTGTGGGGCTACAAATACACTGGCAGACGATCTACTGCCAAATAAGACACTCAGAGATACAATCAATCGCATCTTGGAATCTGGTGGTAACAGTAGTGCTGACAATGCTGGGAGTACATTCCATGTTCAAGATATGGAGTCTGCTCCCTGTCCACAACCCAAGATTCCTTCCCCCACTGTATCTGCTGCATCAAAAGGAGAAGAAAAACATTTACCTTTGAATGAAGAACCTCCAAAGATCCAGGAAACTGAAGATGAGATAAAGCCTGTTCTTCCCCAGCAGCAGATTTTAGACAGAGTGAGAACTACGAAAGTAGTTGATGTGTCTGAAGCCACACATGAGTCAATGAGTGTGAAGGAACCGGCATCACAAGGGAGTGCTCCACTGGTTGAGGAAGAAGTGCAGCAAAGGATGGCTTCTGGGGAGGCAGGAAAGAAAAGGAAAAAGAAGAAAATTAGAGTGCCAGCGAATGACATGCAATGGAGAAACCCTCAAGATCTTGCAGCTGAAAACTATATGATGCCTATGGGCCCTGCTGCTTATAATCCATACTGGAATGGCATGCAACCTAGCATGGGCATGGAAGGATATATGACACCATATGGTGGTCCGATGCCGTATATGGGTTATGGTCCGATGGACATGCCATTTGGGGGTCTTGGTCCACAGGACCCATTTGGTTCTCAAGGCTATATGATTCCCATGGTTCCGCCTCAGAGGGATCTTGCGGACTTTGGTATGGGCATGAATGGGATGAATGGGATGAATGGGATGAATGGGATGAATGGGATGAATGGCATGCATGGGATGAATGGCATGCATGGACCCCCCATCATGAGCAGAGAAGAATTTGAGGCTCGGAAAGCTGATTTGAGAAGGAAGCGTGAAAATGAAAGACGGGGTCAAAGGGAGTTGTCCAAAGATGGGGAGTATGGAAGGGAAGTGAGCAGTGGCGGCGGCGATGTTCCTCCAATGAAACCAAAATCTAAGTCAATGGCCCGATCACCAAGCCCAGATTATACTAATCATCACCGCAGTCACAGACGCGAGAGGCCGTCTCCAGAACGAAGACCCTCACGTGACCCACGTGACCTGGAGCCACAGCCGCCTCCGCGTCCTCCAAAGAGAAACTCCGAGCAGCAGCAGCACCACCACCACCACGACAGAGAACGCGAGCGCGACCGAGATCGAGAACGCGAGAGGGACCACGACCACGACCACGACCACGACCACGAGGATGGTCATCCCCGCCATAAACGCCACCGTTCTGAGTCGTCGTTGGGAAAGCCTTCCGGTCTAACGACAACATCGGCGAAATCTACCTCCTCTATAGCATTAGCTGCGGCAGAAGCGGCGGCGGAGCGGAAGCACAAGGCAAGCGTGTTCTCTCGCATAAGCTTTCCTGAGGGAGAAGTGAGCAAGAAGCGGAAGGCCTCTTCTTCCTCCACTGCTTCTGCCGCCGCTCCTTCTCCGGCGGAGGAGGATAACAGGTCGGCGCATCACCATCACAAGGAGTCGTCGTCGTCCTTGGCGAATGGGTTCTACGAGGAGTATAAGTCGTCGTCTACGGCGGCCAAGGCTGCGAGCAGCGGAGCTGGAAGCAGAAAGACTATGGTCAGAGCTGCTTCCATGGAGTATGAGTCGAGCGACGACGATCGGCACTTCAAGAGGAAGCCGTCGAGGTACGAGGAGTCGCCGCCGAAGGCAGCGGAGCAGGAAGAGCCCTCCAGGCACTCGAGAGGCGGCAAGGATCGGGAACGCAGGCAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005815 GO:0005829 GO:0005856 GO:0006275 GO:0006325 GO:0006464 GO:0006479 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0006974 GO:0006979 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009056 GO:0009057 GO:0009414 GO:0009415 GO:0009628 GO:0009635 GO:0009636 GO:0009651 GO:0009743 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010555 GO:0010556 GO:0015630 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016607 GO:0016740 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019900 GO:0019901 GO:0019941 GO:0030163 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032991 GO:0033554 GO:0034969 GO:0034971 GO:0036211 GO:0042221 GO:0042493 GO:0042542 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046677 GO:0046686 GO:0048518 GO:0048519 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051276 GO:0051603 GO:0051716 GO:0060255 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0070887 GO:0071310 GO:0071704 GO:0071840 GO:0072756 GO:0080090 GO:0080134 GO:0097237 GO:0140096 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902882 GO:1902883 GO:1902884 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DWNN zf-CCHC_2

Protein Analysis

925

Amino Acids

102.66

Weight (kDa)

7.08

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 15, 1545
AasI GACNNNNNNGTC 1 cut(s) 140
Acc16I TGCGCA 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 2 cut(s) 728, 1607
AccBSI CCGCTC 2 cut(s) 2365, 2465
AccI GTMKAC 1 cut(s) 2567
AccII CGCG 6 cut(s) 591, 2049, 2116, 2175, 2181, 2199
AccIII TCCGGA 2 cut(s) 430, 969
AclWI GGATC 4 cut(s) 347, 1234, 1731, 2760
AcoI YGGCCR 1 cut(s) 2574
AcsI RAATTY 5 cut(s) 17, 390, 697, 732, 1850
AcuI CTGAAG 4 cut(s) 417, 477, 1272, 1350
AcvI CACGTG 2 cut(s) 2081, 2090
AfaI GTAC 4 cut(s) 124, 225, 1117, 2687
AflIII ACRYGT 1 cut(s) 854
AgsI TTSAA 5 cut(s) 85, 839, 1130, 1222, 2668
AjiI CACGTC 1 cut(s) 578
AjnI CCWGG 7 cut(s) 226, 346, 535, 655, 1242, 2094, 2729
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
Alw21I GWGCWC 1 cut(s) 1384
Alw26I GTCTC 1 cut(s) 2064
AlwI GGATC 4 cut(s) 347, 1234, 1731, 2760
Ama87I CYCGRG 1 cut(s) 2737
Aor13HI TCCGGA 2 cut(s) 430, 969
AoxI GGCC 8 cut(s) 58, 233, 806, 1531, 1998, 2054, 2433, 2574
ApaI GGGCCC 1 cut(s) 1535
ApoI RAATTY 5 cut(s) 17, 390, 697, 732, 1850
ArsI GACNNNNNNTTYG 4 cut(s) 1634, 1666, 1976, 2008
Asp700I GAANNNNTTC 2 cut(s) 2290, 2540
AspLEI GCGC 3 cut(s) 439, 2181, 2500
AsuHPI GGTGA 8 cut(s) 136, 362, 701, 800, 878, 1998, 2024, 2495
AvaI CYCGRG 1 cut(s) 2737
AvaII GGWCC 8 cut(s) 518, 533, 1610, 1634, 1664, 1672, 1826, 2206
AxyI CCTNAGG 1 cut(s) 2406
BaeGI GKGCMC 1 cut(s) 1535
BanI GGYRCC 1 cut(s) 652
BanII GRGCYC 2 cut(s) 1535, 2727
BauI CACGAG 1 cut(s) 2234
BbrPI CACGTG 2 cut(s) 2081, 2090
BbsI GAAGAC 1 cut(s) 2077
Bbv12I GWGCWC 1 cut(s) 1384
BceAI ACGGC 6 cut(s) 73, 204, 1603, 2041, 2586, 2662
BcgI CGANNNNNNTGC 4 cut(s) 1459, 1493, 1627, 1661
BciT130I CCWGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
BciVI GTATCC 1 cut(s) 977
BcoDI GTCTC 1 cut(s) 2064
BfaI CTAG 2 cut(s) 309, 1572
BfmI CTRYAG 1 cut(s) 2331
BfuI GTATCC 1 cut(s) 977
BglI GCCNNNNNGGC 1 cut(s) 2731
BglII AGATCT 1 cut(s) 1498
Bme1390I CCNGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
Bme18I GGWCC 8 cut(s) 518, 533, 1610, 1634, 1664, 1672, 1826, 2206
BmeT110I CYCGRG 1 cut(s) 2737
BmgBI CACGTC 1 cut(s) 578
BmrFI CCNGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
BmsI GCATC 9 cut(s) 231, 424, 907, 1080, 1199, 1376, 1512, 1605, 2509
BoxI GACNNNNGTC 1 cut(s) 2615
BpiI GAAGAC 1 cut(s) 2077
BplI GAGNNNNNCTC 4 cut(s) 1477, 1509, 2044, 2076
BpmI CTGGAG 3 cut(s) 2046, 2117, 2713
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 2 cut(s) 1479, 1671
BsaAI YACGTR 2 cut(s) 2081, 2090
BsaBI GATNNNNATC 1 cut(s) 2244
BsaJI CCNNGG 7 cut(s) 498, 514, 656, 1704, 2529, 2577, 2631
BsaWI WCCGGW 3 cut(s) 430, 969, 2294
BsaXI ACNNNNNCTCC 6 cut(s) 2507, 2537, 2543, 2573, 2627, 2657
Bse118I RCCGGY 1 cut(s) 1363
Bse1I ACTGG 4 cut(s) 733, 1026, 1392, 1559
Bse21I CCTNAGG 1 cut(s) 2406
Bse3DI GCAATG 1 cut(s) 1487
Bse8I GATNNNNATC 1 cut(s) 2244
BseAI TCCGGA 2 cut(s) 430, 969
BseBI CCWGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
BseDI CCNNGG 7 cut(s) 498, 514, 656, 1704, 2529, 2577, 2631
BseJI GATNNNNATC 1 cut(s) 2244
BseMI GCAATG 1 cut(s) 1487
BseMII CTCAG 5 cut(s) 870, 1066, 1730, 2262, 2397
BseNI ACTGG 4 cut(s) 733, 1026, 1392, 1559
BseRI GAGGAG 5 cut(s) 609, 2317, 2495, 2564, 2705
BseSI GKGCMC 1 cut(s) 1535
BseYI CCCAGC 2 cut(s) 1109, 1278
BsgI GTGCAG 2 cut(s) 431, 1424
Bsh1236I CGCG 6 cut(s) 591, 2049, 2116, 2175, 2181, 2199
Bsh1285I CGRYCG 2 cut(s) 2185, 2659
BshFI GGCC 8 cut(s) 60, 235, 808, 1533, 2000, 2056, 2435, 2576
BshNI GGYRCC 1 cut(s) 652
BsiEI CGRYCG 2 cut(s) 2185, 2659
BsiHKAI GWGCWC 1 cut(s) 1384
BsiHKCI CYCGRG 1 cut(s) 2737
BsiSI CCGG 5 cut(s) 431, 970, 1364, 2295, 2474
BslFI GGGAC 2 cut(s) 504, 2219
BsmAI GTCTC 1 cut(s) 2064
BsmBI CGTCTC 1 cut(s) 2064
BsmFI GGGAC 2 cut(s) 504, 2219
BsnI GGCC 8 cut(s) 60, 235, 808, 1533, 2000, 2056, 2435, 2576
BsoBI CYCGRG 1 cut(s) 2737
Bsp120I GGGCCC 1 cut(s) 1531
Bsp1286I GDGCHC 3 cut(s) 1384, 1535, 2727
Bsp13I TCCGGA 2 cut(s) 430, 969
Bsp19I CCATGG 2 cut(s) 1704, 2631
BspANI GGCC 8 cut(s) 60, 235, 808, 1533, 2000, 2056, 2435, 2576
BspCNI CTCAG 5 cut(s) 871, 1065, 1729, 2263, 2398
BspEI TCCGGA 2 cut(s) 430, 969
BspFNI CGCG 6 cut(s) 591, 2049, 2116, 2175, 2181, 2199
BspHI TCATGA 1 cut(s) 1836
BspPI GGATC 4 cut(s) 347, 1234, 1731, 2760
BspQI GCTCTTC 1 cut(s) 2715
BspT107I GGYRCC 1 cut(s) 652
BsrBI CCGCTC 2 cut(s) 2365, 2465
BsrDI GCAATG 1 cut(s) 1487
BsrFI RCCGGY 1 cut(s) 1363
BsrI ACTGG 4 cut(s) 733, 1026, 1392, 1559
BssAI RCCGGY 1 cut(s) 1363
BssECI CCNNGG 7 cut(s) 498, 514, 656, 1704, 2529, 2577, 2631
BssSI CACGAG 1 cut(s) 2234
BssT1I CCWWGG 6 cut(s) 498, 514, 1704, 2529, 2577, 2631
Bst2BI CACGAG 1 cut(s) 2234
Bst2UI CCWGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
Bst4CI ACNGT 3 cut(s) 1094, 1180, 2267
Bst6I CTCTTC 3 cut(s) 159, 2443, 2715
BstAPI GCANNNNNTGC 2 cut(s) 939, 2342
BstBAI YACGTR 2 cut(s) 2081, 2090
BstC8I GCNNGC 9 cut(s) 405, 1467, 1565, 1802, 1820, 2177, 2380, 2587, 2765
BstDEI CTNAG 8 cut(s) 112, 738, 879, 1052, 1716, 1989, 2271, 2406
BstDSI CCRYGG 2 cut(s) 1704, 2631
BstENI CCTNNNNNAGG 1 cut(s) 540
BstFNI CGCG 6 cut(s) 591, 2049, 2116, 2175, 2181, 2199
BstHHI GCGC 3 cut(s) 439, 2181, 2500
BstMAI GTCTC 1 cut(s) 2064
BstMCI CGRYCG 2 cut(s) 2185, 2659
BstNI CCWGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
BstNSI RCATGY 5 cut(s) 1480, 1567, 1648, 1804, 1822
BstPAI GACNNNNGTC 1 cut(s) 2615
BstSCI CCNGG 7 cut(s) 226, 346, 535, 655, 1242, 2094, 2729
BstSFI CTRYAG 1 cut(s) 2331
BstSLI GKGCMC 1 cut(s) 1535
BstUI CGCG 6 cut(s) 591, 2049, 2116, 2175, 2181, 2199
BstV2I GAAGAC 1 cut(s) 2077
BstX2I RGATCY 3 cut(s) 1239, 1498, 1723
BstXI CCANNNNNNTGG 2 cut(s) 751, 2240
BstYI RGATCY 3 cut(s) 1239, 1498, 1723
Bsu36I CCTNAGG 1 cut(s) 2406
BsuI GTATCC 1 cut(s) 977
BsuRI GGCC 8 cut(s) 60, 235, 808, 1533, 2000, 2056, 2435, 2576
BtgI CCRYGG 2 cut(s) 1704, 2631
BtgZI GCGATG 2 cut(s) 52, 1977
BtrI CACGTC 1 cut(s) 578
BtsI GCAGTG 4 cut(s) 673, 886, 1957, 2448
Cac8I GCNNGC 9 cut(s) 405, 1467, 1565, 1802, 1820, 2177, 2380, 2587, 2765
CciI TCATGA 1 cut(s) 1836
CfoI GCGC 3 cut(s) 439, 2181, 2500
Cfr10I RCCGGY 1 cut(s) 1363
CseI GACGC 2 cut(s) 2055, 2105
Csp6I GTAC 4 cut(s) 123, 224, 1116, 2686
CviQI GTAC 4 cut(s) 123, 224, 1116, 2686
DdeI CTNAG 8 cut(s) 112, 738, 879, 1052, 1716, 1989, 2271, 2406
DraI TTTAAA 1 cut(s) 946
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 2574
Eam1104I CTCTTC 3 cut(s) 159, 2443, 2715
EarI CTCTTC 3 cut(s) 159, 2443, 2715
EciI GGCGGA 3 cut(s) 1701, 2375, 2492
Eco130I CCWWGG 6 cut(s) 498, 514, 1704, 2529, 2577, 2631
Eco147I AGGCCT 2 cut(s) 235, 2435
Eco24I GRGCYC 2 cut(s) 1535, 2727
Eco47I GGWCC 8 cut(s) 518, 533, 1610, 1634, 1664, 1672, 1826, 2206
Eco57I CTGAAG 4 cut(s) 417, 477, 1272, 1350
Eco72I CACGTG 2 cut(s) 2081, 2090
Eco81I CCTNAGG 1 cut(s) 2406
Eco88I CYCGRG 1 cut(s) 2737
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 3 cut(s) 518, 1532, 1672
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 7 cut(s) 226, 346, 535, 655, 1242, 2094, 2729
EcoT14I CCWWGG 6 cut(s) 498, 514, 1704, 2529, 2577, 2631
EcoT22I ATGCAT 2 cut(s) 1806, 1824
EcoT38I GRGCYC 2 cut(s) 1535, 2727
ErhI CCWWGG 6 cut(s) 498, 514, 1704, 2529, 2577, 2631
Esp3I CGTCTC 1 cut(s) 2064
FalI AAGNNNNNCTT 6 cut(s) 156, 188, 926, 958, 1402, 1434
FaqI GGGAC 2 cut(s) 504, 2219
FauI CCCGC 2 cut(s) 111, 2258
FauNDI CATATG 1 cut(s) 1603
FblI GTMKAC 1 cut(s) 2567
FriOI GRGCYC 2 cut(s) 1535, 2727
FspBI CTAG 2 cut(s) 309, 1572
FspI TGCGCA 1 cut(s) 438
GlaI GCGC 3 cut(s) 438, 2180, 2499
GsaI CCCAGC 2 cut(s) 1113, 1282
GsuI CTGGAG 3 cut(s) 2046, 2117, 2713
HaeIII GGCC 8 cut(s) 60, 235, 808, 1533, 2000, 2056, 2435, 2576
HapII CCGG 5 cut(s) 431, 970, 1364, 2295, 2474
HgaI GACGC 2 cut(s) 2055, 2105
HhaI GCGC 3 cut(s) 439, 2181, 2500
Hin6I GCGC 3 cut(s) 437, 2179, 2498
HinP1I GCGC 3 cut(s) 437, 2179, 2498
HindIII AAGCTT 2 cut(s) 949, 2398
HpaII CCGG 5 cut(s) 431, 970, 1364, 2295, 2474
HphI GGTGA 8 cut(s) 136, 362, 701, 800, 878, 1998, 2024, 2495
Hpy166II GTNNAC 5 cut(s) 224, 845, 1154, 1667, 2568
Hpy8I GTNNAC 5 cut(s) 224, 845, 1154, 1667, 2568
Hpy99I CGWCG 8 cut(s) 143, 2281, 2524, 2527, 2566, 2654, 2657, 2683
HpyCH4III ACNGT 3 cut(s) 1094, 1180, 2267
HpyCH4IV ACGT 4 cut(s) 577, 856, 2080, 2089
HpyF3I CTNAG 8 cut(s) 112, 738, 879, 1052, 1716, 1989, 2271, 2406
HpySE526I ACGT 4 cut(s) 577, 856, 2080, 2089
HspAI GCGC 3 cut(s) 437, 2179, 2498
Kpn2I TCCGGA 2 cut(s) 430, 969
LguI GCTCTTC 1 cut(s) 2715
LmnI GCTCC 8 cut(s) 433, 1150, 1387, 2098, 2362, 2470, 2594, 2713
LweI GCATC 9 cut(s) 231, 424, 907, 1080, 1199, 1376, 1512, 1605, 2509
MaeI CTAG 2 cut(s) 309, 1572
MaeII ACGT 4 cut(s) 577, 856, 2080, 2089
MaeIII GTNAC 6 cut(s) 142, 250, 1088, 2039, 2081, 2090
MbiI CCGCTC 2 cut(s) 2365, 2465
MflI RGATCY 3 cut(s) 1239, 1498, 1723
MhlI GDGCHC 3 cut(s) 1384, 1535, 2727
MluCI AATT 7 cut(s) 17, 390, 697, 732, 776, 1455, 1850
MlyI GAGTC 6 cut(s) 1148, 1352, 2282, 2525, 2651, 2702
Mph1103I ATGCAT 2 cut(s) 1806, 1824
MroI TCCGGA 2 cut(s) 430, 969
MroXI GAANNNNTTC 2 cut(s) 2290, 2540
MseI TTAA 5 cut(s) 21, 474, 708, 717, 945
MslI CAYNNNNRTG 4 cut(s) 749, 992, 1580, 2238
MspA1I CMGCKG 4 cut(s) 323, 1508, 2592, 2711
MspI CCGG 5 cut(s) 431, 970, 1364, 2295, 2474
MspR9I CCNGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
MvaI CCWGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
MvnI CGCG 6 cut(s) 591, 2049, 2116, 2175, 2181, 2199
NcoI CCATGG 2 cut(s) 1704, 2631
NdeI CATATG 1 cut(s) 1603
NmuCI GTSAC 4 cut(s) 142, 2039, 2081, 2090
NsbI TGCGCA 1 cut(s) 438
NsiI ATGCAT 2 cut(s) 1806, 1824
NspI RCATGY 5 cut(s) 1480, 1567, 1648, 1804, 1822
PaeI GCATGC 3 cut(s) 1567, 1804, 1822
PaeR7I CTCGAG 1 cut(s) 2737
PagI TCATGA 1 cut(s) 1836
PceI AGGCCT 2 cut(s) 235, 2435
PciSI GCTCTTC 1 cut(s) 2715
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 2086, 2531
PdmI GAANNNNTTC 2 cut(s) 2290, 2540
PfeI GAWTC 8 cut(s) 41, 101, 190, 257, 326, 1079, 1165, 1699
PflFI GACNNNGTC 2 cut(s) 137, 1902
PflMI CCANNNNNTGG 2 cut(s) 728, 1607
PfoI TCCNGGA 1 cut(s) 1242
Ple19I CGATCG 1 cut(s) 2659
PleI GAGTC 6 cut(s) 1147, 1351, 2281, 2524, 2650, 2701
PmaCI CACGTG 2 cut(s) 2081, 2090
PmlI CACGTG 2 cut(s) 2081, 2090
PpsI GAGTC 6 cut(s) 1147, 1351, 2281, 2524, 2650, 2701
Ppu21I YACGTR 2 cut(s) 2081, 2090
PpuMI RGGWCCY 2 cut(s) 518, 1672
PshAI GACNNNNGTC 1 cut(s) 2615
PsiI TTATAA 2 cut(s) 15, 1545
Psp5II RGGWCCY 2 cut(s) 518, 1672
Psp6I CCWGG 7 cut(s) 226, 346, 535, 655, 1242, 2094, 2729
PspCI CACGTG 2 cut(s) 2081, 2090
PspFI CCCAGC 2 cut(s) 1109, 1278
PspGI CCWGG 7 cut(s) 226, 346, 535, 655, 1242, 2094, 2729
PspOMI GGGCCC 1 cut(s) 1531
PspPPI RGGWCCY 2 cut(s) 518, 1672
PsuI RGATCY 3 cut(s) 1239, 1498, 1723
PsyI GACNNNGTC 2 cut(s) 137, 1902
PvuI CGATCG 1 cut(s) 2659
PvuII CAGCTG 2 cut(s) 323, 1508
RsaI GTAC 4 cut(s) 124, 225, 1117, 2687
RsaNI GTAC 4 cut(s) 123, 224, 1116, 2686
RseI CAYNNNNRTG 4 cut(s) 749, 992, 1580, 2238
SapI GCTCTTC 1 cut(s) 2715
SaqAI TTAA 5 cut(s) 21, 474, 708, 717, 945
SchI GAGTC 6 cut(s) 1148, 1352, 2282, 2525, 2651, 2702
ScrFI CCNGG 7 cut(s) 228, 348, 537, 657, 1244, 2096, 2731
SduI GDGCHC 3 cut(s) 1384, 1535, 2727
SfaNI GCATC 9 cut(s) 231, 424, 907, 1080, 1199, 1376, 1512, 1605, 2509
SfcI CTRYAG 1 cut(s) 2331
Sfr274I CTCGAG 1 cut(s) 2737
SinI GGWCC 8 cut(s) 518, 533, 1610, 1634, 1664, 1672, 1826, 2206
SlaI CTCGAG 1 cut(s) 2737
SmiMI CAYNNNNRTG 4 cut(s) 749, 992, 1580, 2238
SmlI CTYRAG 3 cut(s) 1494, 1686, 2737
SmoI CTYRAG 3 cut(s) 1494, 1686, 2737
SphI GCATGC 3 cut(s) 1567, 1804, 1822
Sse9I AATT 7 cut(s) 17, 390, 697, 732, 776, 1455, 1850
SseBI AGGCCT 2 cut(s) 235, 2435
SspMI CTAG 2 cut(s) 309, 1572
StuI AGGCCT 2 cut(s) 235, 2435
StyD4I CCNGG 7 cut(s) 226, 346, 535, 655, 1242, 2094, 2729
StyI CCWWGG 6 cut(s) 498, 514, 1704, 2529, 2577, 2631
TaaI ACNGT 3 cut(s) 1094, 1180, 2267
TaiI ACGT 4 cut(s) 580, 859, 2083, 2092
TaqI TCGA 6 cut(s) 694, 702, 2191, 2645, 2681, 2738
TaqII GACCGA 1 cut(s) 2199
TasI AATT 7 cut(s) 17, 390, 697, 732, 776, 1455, 1850
TatI WGTACW 1 cut(s) 1115
TfiI GAWTC 8 cut(s) 41, 101, 190, 257, 326, 1079, 1165, 1699
Tru1I TTAA 5 cut(s) 21, 474, 708, 717, 945
Tru9I TTAA 5 cut(s) 21, 474, 708, 717, 945
TseFI GTSAC 4 cut(s) 142, 2039, 2081, 2090
Tsp45I GTSAC 4 cut(s) 142, 2039, 2081, 2090
TspGWI ACGGA 1 cut(s) 642
Tth111I GACNNNGTC 2 cut(s) 137, 1902
Van91I CCANNNNNTGG 2 cut(s) 728, 1607
VpaK11BI GGWCC 8 cut(s) 518, 533, 1610, 1634, 1664, 1672, 1826, 2206
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 5 cut(s) 17, 390, 697, 732, 1850
XceI RCATGY 5 cut(s) 1480, 1567, 1648, 1804, 1822
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XhoI CTCGAG 1 cut(s) 2737
XmiI GTMKAC 1 cut(s) 2567
XmnI GAANNNNTTC 2 cut(s) 2290, 2540
XspI CTAG 2 cut(s) 309, 1572
Zsp2I ATGCAT 2 cut(s) 1806, 1824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.