Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002185

Physical Location & Seq

Genomic Coordinates Reverse (-)

1108719 .. 1117613

Gene Structure 8895 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1014700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3198 bp

ATGGGAGTTCCGGCGTTCTACCGGTGGTTGGCGGAGAAGTACCCGCTCGTGATTGTCGACGTGGTGGAAGAAGAGCCCGTCGTGATCGATGACGTATCGATTCCGGTCGACACCAGTAAACCAAATCCTAATGGGTTGGAATACGACAATCTGTATCTCGACATGAACGGGATTATTCACCCCTGTTTCCACCCAGAAGATAGGCCTTCTCCCTCGACCTTCGACGAGGTATTCCAGTGCATGTTTGATTACATTGACAGACTGTTTGTGATGGTTCGGCCTCGAAAGCTATTGTACATGGCGATTGATGGTGTTGCCCCCAGGGCAAAAATGAATCAACAGCGGTCTCGGCGATTTAGGGCTGCGAAAGATGCAGCAGACGCGGCAGCTGAAGAAGAAAGGCTGCGTGAGGAGTTTGAAATGGAGGGGAGAAAGCTTCCTCCCAAACAAGACTCACAAGTTGTTGATTCCAATGTCATCACTCCTGGAACTGAATTTATGGCTGTTCTGTCAGTAGCATTGCAGTATTACATCCATCTTAGATTAAACAATGACCCAGGATGGAAAAATGTTAAGGTTATTCTTTCTGATGCAAATGTTCCAGGTGAAGGTGAACACAAAATTATGTCCTATATTCGTCTTCAAAGAAATCTTCCTGGTTATGATCCAAATACACGCCATTGTCTGTATGGTTTGGATGCTGATTTGATTATGCTCGCTTTGGCTACCCATGAGGTTCATTTTTCAATTCTTCGAGAGGTTGTATTCACACCCGGACAGCAAGACAAATGCTTCATATGTGGTCAGGTGGGTCATTTAGCAGCAAATTGTGAAGGTAAGGCGAAACGGAAGGCTGGAGAGTTTGATGAGAAAGCTGATGGTGAGGCTGTGCCCAAGAAGCCTTACCAGTTTCTCAACATCTGGACTCTTAGAGAATATTTGGAATATGAGATGCGAATTCCCAACACCCCATTCGAGATTGATTTTGAGTGCATTGTGGACGATTTCATATTCATGTGCTTCTTCGTTGGTAACGATTTCTTGCCACACATGCCCACGCTGGAGATTCGTGAGGGTGCGATCAATTTGCTCTTGGCTGTATACAAGAAGGAATTTAGGGCATTGGGTGGATATCTGACTGATGGAAGCAAGCCGAATTTAAGTCGGGTAGAGCATTTCATTCAAGCAGTTGGATCTTATGAAGATAAAATATTTCAGAAAAGAGCTCGATTGCATCAGCGTCAGGCAGATAGAATAAAGCGTGATAAAAGGCAATTAAGAAGAGGAGATGATGCAGCGCCCCAAGTGCAACCTGATTCTTTGGTGCCAGTTTCTCGGTTTCAGGGATCTCGTCTTGCTTCGGGTCATACAGCTTCACCTTATCAACAGTCTGGAAAATCTAATGATCAAGGAAATCCTCGAAAGGTTGCACGTTTGTCTTCGGAGATGTCAACTGTTGGTGCTGCTATTGTTGAAGCCGAGGACAATCTTGAACTAGATGAACATGGAAACAAAGAAGACTTGAAAGCAAAGCTGAAGGAGATTCTTCGTGAGAAATCTGATGCTTTTAACTCTGGAAATCCCGACGTAGACAAGGTTAAATTGGGAGAACCTGGATGGAAAGATAGGTATTACGAAGAAAAGTTTTCTGCAAAAACTCCTGAAGAACGTGATGCAATTAGAAAGGATGTTGTTTTGCGATACACTGAGGGTCTATGTTGGGTGATGCACTATTATTATGAAGGTGTTTGCTCGTGGCAGTGGTTTTACCCCTATCACTATGCACCCTTTGCTTCTGATCTGAAAGACCTTGGTCAGCTAAATATAAGTTTCGACTTGGGCACTCCTTTTAAACCGTTCAATCAGCTATTGGGGGTTTTTCCTGCTGCAAGCTCCCATGCTCTTCCTGAGCACTACAGGAAGTTGATGACTGATCCAAATTCACCTATCATTGACTTTTACCCTATTGACTTTGATGTGGATATGAATGGGAAGCGGTATGCATGGCAGGGTATTGCAAAGTTACCTTTTATTGATGAAGTCCGCCTTCTAGCTGAGGTTGAAAAACTTGAACATACATTGACGGAGGAGGAGACCCGGAGAAACAGTGTGATGTTCGAGATGCTTTTTGTTGCATCGTCTCATCCTCTATCTGTATGCATATATTCTCTGGACAATCGATGTAAACAGTTAACAGACAAAGAGCGAGTTGAAGTCAAGGAACGGATTGATCCCAAGTACAGTGAGGGGATGAACGGTTATCTGTCTCCATGTACTGGGGAGCCTTGCCCTCCGGTTTTCCGGTCTCCTGTTGACAGCATGGAGGATATTATGGACAACCAAGTAATATGTGCGATATACATACTACCGGATGTACATAAGCATATAACTCGACCTCCAGCAGGTGTTATATTCCCGAAGAAGACCGTTACAGTGGGGGATGTGAAGCCTTTACCTCCTTTATGGCATGAAGAAACCGGGCGAAAGCCATGGCATGAAAATTCTGGGCGGACGCCATACGAAAGCTCCCATTCCAGGCAGAACCCTGCAGGAGCTATTTCTGGAAGACAGCTCGGGGCAGCGGCACATCGCCTTGTTGTTAATAGCTTACAGGCTAAAACGGATTCCAATGGGTATGGCAATCACATGCATTCGCATCGACCATCTTATGCCGAGCCTCAGTATTATCCACCGCCTCCTTCCTATGCAAACAACGGAAGCTATGATCAGGGACACAGTAGAATGCCACCACCTAGGACAATCCACCATCCTCAACAGCAACCCCGAGCTTCGAATATTAGTCACGGTGACCGTGGGTATAATGTCGCGCAATCTTCAACGGCTGTCCACTTTCAGAACAGGCCTCACACTCAGCACCATGAAAGGAATTCTGGGCATGTATACTACGAATCAGGAAATCACCAGAACGGTGGGCCAATCTACCCTCAGAGACCTATTTTACAGACTCCGCCGGGAGTCGGCGGTGCTCCTTATCCAGGTGGCTATAACTACTATCAGAGTTACCAACCATCTGCAGCGTCTGGCCATCATGGTTATGATGACTGGGTTCCACCGGTCGGCCATAACCCGGGCAGAGGTTATGGCCGCCCTCAGCAATCCGGAAACCAATTCTCTGGATTGGACAGGAGAGGAAATAGGAGGCCACCTGCACCCCAGTATGGTTATGGCCACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000966 GO:0000967 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004534 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008409 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0022613 GO:0034470 GO:0034471 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0071025 GO:0071027 GO:0071029 GO:0071035 GO:0071046 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03008 ko03018 map03008 map03018

Pfam Domains

Protein Families

XRN_N zf-CCHC

Protein Analysis

1066

Amino Acids

121.0

Weight (kDa)

6.37

Isoelectric Point (pI)

49.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XRN_N	PF03159	1 - 253	1e-101	XRN 5'-3' exonuclease N-terminus
XRN_M	PF17846	327 - 814	7.5e-165	Xrn1 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002185)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75660 AT5G42540 AT5G42540
fragaria_vesca	FvH4_2g38700 FvH4_2g38700 FvH4_5g21470
malus_domestica	MD00G1128500.v1.1 MD08G1014700.v1.1 MD15G1014000.v1.1
prunus_persica	Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1
pyrus_communis	pycom08g01310 pycom15g01210
rosa_chinensis	RchiOBHm_Chr5g0009961 RchiOBHm_Chr6g0302611
rosa_laevigata	RLG00000011157 RLG00000031714
rosa_multiflora	Rmu_sc0027322.1_g000001 Rmu_sc0027323.1_g000001 Rmu_sc0039467.1_g000001 Rmu_sc0039989.1_g000001 Rmu_sc0041627.1_g000001
rosa_roxburghii	Rroxscaffold_1G00066230 Rroxscaffold_7G00165520
rosa_rugosa	Rorug04G0447300 Rorug06G0315300 Rorug06G0315400
rosa_samantha	Rh5AG078800 Rh5BG073500 Rh5CG086100 Rh5DG074200 Rh5DG336700 Rh6AG428700 Rh6BG473800 Rh6CG441000 Rh6DG427700
rosa_wichuraiana	Rw6G037100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2393, 3175
Acc36I	ACCTGC	2 cut(s)	2393, 3175
AccB1I	GGYRCC	1 cut(s)	1324
AccB7I	CCANNNNNTGG	1 cut(s)	2276
AccBSI	CCGCTC	1 cut(s)	46
AccI	GTMKAC	5 cut(s)	57, 108, 1101, 1590, 2901
AccII	CGCG	2 cut(s)	383, 2828
AccIII	TCCGGA	1 cut(s)	3119
AclWI	GGATC	5 cut(s)	659, 1201, 1354, 1928, 2225
AcoI	YGGCCR	4 cut(s)	3043, 3079, 3103, 3187
AcsI	RAATTY	7 cut(s)	494, 957, 1112, 1156, 1939, 2500, 2887
AcuI	CTGAAG	3 cut(s)	411, 1556, 1683
AcyI	GRCGYC	1 cut(s)	2513
AfaI	GTAC	5 cut(s)	41, 296, 2240, 2275, 2376
AgeI	ACCGGT	2 cut(s)	21, 3073
AjiI	CACGTC	1 cut(s)	61
AjnI	CCWGG	8 cut(s)	320, 484, 556, 601, 655, 1612, 2534, 2995
AjuI	GAANNNNNNNTTGG	2 cut(s)	956, 988
Alw21I	GWGCWC	3 cut(s)	1228, 1914, 2989
Alw26I	GTCTC	6 cut(s)	351, 2087, 2145, 2271, 2310, 2944
AlwI	GGATC	5 cut(s)	659, 1201, 1354, 1928, 2225
AlwNI	CAGNNNCTG	1 cut(s)	3041
Ama87I	CYCGRG	3 cut(s)	2573, 2784, 3088
Aor13HI	TCCGGA	1 cut(s)	3119
AoxI	GGCC	9 cut(s)	203, 278, 2861, 2933, 3043, 3079, 3103, 3161, 3187
ApoI	RAATTY	7 cut(s)	494, 957, 1112, 1156, 1939, 2500, 2887
ArsI	GACNNNNNNTTYG	2 cut(s)	248, 280
AsiGI	ACCGGT	2 cut(s)	21, 3073
AspA2I	CCTAGG	1 cut(s)	2753
AspLEI	GCGC	2 cut(s)	1300, 2830
AspS9I	GGNCC	1 cut(s)	2933
AsuC2I	CCSGG	6 cut(s)	774, 2098, 2479, 2973, 3089, 3090
AsuHPI	GGTGA	9 cut(s)	170, 617, 623, 893, 1368, 1735, 1935, 2819, 2912
AsuII	TTCGAA	1 cut(s)	2792
AvaI	CYCGRG	3 cut(s)	2573, 2784, 3088
AvrII	CCTAGG	1 cut(s)	2753
BaeGI	GKGCMC	2 cut(s)	894, 1844
BalI	TGGCCA	2 cut(s)	3045, 3189
BanI	GGYRCC	1 cut(s)	1324
BanII	GRGCYC	2 cut(s)	78, 1228
BauI	CACGAG	2 cut(s)	47, 1753
BbsI	GAAGAC	5 cut(s)	632, 1431, 1524, 2429, 2572
Bbv12I	GWGCWC	3 cut(s)	1228, 1914, 2989
BbvCI	CCTCAGC	2 cut(s)	2055, 3111
BceAI	ACGGC	1 cut(s)	2856
BcgI	CGANNNNNNTGC	6 cut(s)	1069, 1103, 2372, 2406, 2639, 2673
BciT130I	CCWGG	8 cut(s)	322, 486, 558, 603, 657, 1614, 2536, 2997
BclI	TGATCA	2 cut(s)	1405, 2725
BcnI	CCSGG	6 cut(s)	774, 2098, 2479, 2973, 3089, 3090
BcoDI	GTCTC	6 cut(s)	351, 2087, 2145, 2271, 2310, 2944
BfaI	CTAG	3 cut(s)	1496, 2051, 2754
BfmI	CTRYAG	3 cut(s)	1915, 2547, 3033
BfoI	RGCGCY	1 cut(s)	1301
BfuAI	ACCTGC	2 cut(s)	2393, 3175
BglI	GCCNNNNNGGC	1 cut(s)	323
BlnI	CCTAGG	1 cut(s)	2753
BmeT110I	CYCGRG	3 cut(s)	2573, 2784, 3088
BmgBI	CACGTC	1 cut(s)	61
BmgT120I	GGNCC	1 cut(s)	2933
BmiI	GGNNCC	3 cut(s)	1326, 2283, 3069
BmrI	ACTGGG	3 cut(s)	2286, 3073, 3169
BmuI	ACTGGG	3 cut(s)	2286, 3073, 3169
BoxI	GACNNNNGTC	1 cut(s)	1812
BpiI	GAAGAC	5 cut(s)	632, 1431, 1524, 2429, 2572
BpmI	CTGGAG	3 cut(s)	876, 1082, 2382
Bpu10I	CCTNAGC	3 cut(s)	1908, 2055, 3111
Bpu14I	TTCGAA	1 cut(s)	2792
BpuMI	CCSGG	6 cut(s)	774, 2098, 2479, 2973, 3089, 3090
Bsa29I	ATCGAT	3 cut(s)	87, 98, 2179
BsaHI	GRCGYC	1 cut(s)	2513
BsaI	GGTCTC	4 cut(s)	351, 2087, 2310, 2944
BsaJI	CCNNGG	9 cut(s)	320, 321, 556, 1479, 1810, 2489, 2753, 2812, 3088
BsaWI	WCCGGW	7 cut(s)	21, 103, 2293, 2301, 2368, 3073, 3119
BsaXI	ACNNNNNCTCC	6 cut(s)	2092, 2122, 2380, 2410, 2510, 2540
Bse118I	RCCGGY	2 cut(s)	21, 3073
Bse1I	ACTGG	7 cut(s)	114, 235, 907, 1328, 2281, 3068, 3175
Bse3DI	GCAATG	1 cut(s)	518
BseAI	TCCGGA	1 cut(s)	3119
BseBI	CCWGG	8 cut(s)	322, 486, 558, 603, 657, 1614, 2536, 2997
BseCI	ATCGAT	3 cut(s)	87, 98, 2179
BseDI	CCNNGG	9 cut(s)	320, 321, 556, 1479, 1810, 2489, 2753, 2812, 3088
BseMI	GCAATG	1 cut(s)	518
BseMII	CTCAG	7 cut(s)	1698, 1899, 2046, 2693, 2885, 2960, 3125
BseNI	ACTGG	7 cut(s)	114, 235, 907, 1328, 2281, 3068, 3175
BseRI	GAGGAG	4 cut(s)	425, 1299, 2102, 2105
BseSI	GKGCMC	2 cut(s)	894, 1844
BsgI	GTGCAG	1 cut(s)	3153
Bsh1236I	CGCG	2 cut(s)	383, 2828
Bsh1285I	CGRYCG	2 cut(s)	108, 3078
BshFI	GGCC	9 cut(s)	205, 280, 2863, 2935, 3045, 3081, 3105, 3163, 3189
BshNI	GGYRCC	1 cut(s)	1324
BshTI	ACCGGT	2 cut(s)	21, 3073
BshVI	ATCGAT	3 cut(s)	87, 98, 2179
BsiEI	CGRYCG	2 cut(s)	108, 3078
BsiHKAI	GWGCWC	3 cut(s)	1228, 1914, 2989
BsiHKCI	CYCGRG	3 cut(s)	2573, 2784, 3088
BslFI	GGGAC	1 cut(s)	2745
BsmAI	GTCTC	6 cut(s)	351, 2087, 2145, 2271, 2310, 2944
BsmBI	CGTCTC	1 cut(s)	2145
BsmFI	GGGAC	1 cut(s)	2745
BsmI	GAATGC	2 cut(s)	2650, 2748
BsnI	GGCC	9 cut(s)	205, 280, 2863, 2935, 3045, 3081, 3105, 3163, 3189
Bso31I	GGTCTC	4 cut(s)	351, 2087, 2310, 2944
BsoBI	CYCGRG	3 cut(s)	2573, 2784, 3088
Bsp119I	TTCGAA	1 cut(s)	2792
Bsp1286I	GDGCHC	6 cut(s)	78, 894, 1228, 1844, 1914, 2989
Bsp13I	TCCGGA	1 cut(s)	3119
Bsp1407I	TGTACA	2 cut(s)	294, 2374
Bsp19I	CCATGG	1 cut(s)	2489
BspANI	GGCC	9 cut(s)	205, 280, 2863, 2935, 3045, 3081, 3105, 3163, 3189
BspCNI	CTCAG	7 cut(s)	1699, 1900, 2047, 2692, 2884, 2959, 3124
BspDI	ATCGAT	3 cut(s)	87, 98, 2179
BspEI	TCCGGA	1 cut(s)	3119
BspFNI	CGCG	2 cut(s)	383, 2828
BspLI	GGNNCC	3 cut(s)	1326, 2283, 3069
BspMAI	CTGCAG	2 cut(s)	2551, 3037
BspMI	ACCTGC	2 cut(s)	2393, 3175
BspPI	GGATC	5 cut(s)	659, 1201, 1354, 1928, 2225
BspQI	GCTCTTC	2 cut(s)	66, 1908
BspT104I	TTCGAA	1 cut(s)	2792
BspT107I	GGYRCC	1 cut(s)	1324
BspTNI	GGTCTC	4 cut(s)	351, 2087, 2310, 2944
BsrBI	CCGCTC	1 cut(s)	46
BsrDI	GCAATG	1 cut(s)	518
BsrFI	RCCGGY	2 cut(s)	21, 3073
BsrGI	TGTACA	2 cut(s)	294, 2374
BsrI	ACTGG	7 cut(s)	114, 235, 907, 1328, 2281, 3068, 3175
BssAI	RCCGGY	2 cut(s)	21, 3073
BssECI	CCNNGG	9 cut(s)	320, 321, 556, 1479, 1810, 2489, 2753, 2812, 3088
BssNAI	GTATAC	2 cut(s)	1102, 2902
BssNI	GRCGYC	1 cut(s)	2513
BssSI	CACGAG	2 cut(s)	47, 1753
BssT1I	CCWWGG	3 cut(s)	1810, 2489, 2753
Bst1107I	GTATAC	2 cut(s)	1102, 2902
Bst2BI	CACGAG	2 cut(s)	47, 1753
Bst2UI	CCWGG	8 cut(s)	322, 486, 558, 603, 657, 1614, 2536, 2997
Bst6I	CTCTTC	3 cut(s)	66, 1276, 1908
BstACI	GRCGYC	1 cut(s)	2513
BstAPI	GCANNNNNTGC	1 cut(s)	1790
BstAUI	TGTACA	2 cut(s)	294, 2374
BstBI	TTCGAA	1 cut(s)	2792
BstC8I	GCNNGC	3 cut(s)	717, 1151, 1891
BstDEI	CTNAG	9 cut(s)	539, 929, 1707, 1908, 2055, 2679, 2871, 2946, 3111
BstDSI	CCRYGG	2 cut(s)	2489, 2812
BstEII	GGTNACC	1 cut(s)	2807
BstENI	CCTNNNNNAGG	2 cut(s)	2400, 2994
BstFNI	CGCG	2 cut(s)	383, 2828
BstH2I	RGCGCY	1 cut(s)	1301
BstHHI	GCGC	2 cut(s)	1300, 2830
BstMAI	GTCTC	6 cut(s)	351, 2087, 2145, 2271, 2310, 2944
BstMCI	CGRYCG	2 cut(s)	108, 3078
BstNI	CCWGG	8 cut(s)	322, 486, 558, 603, 657, 1614, 2536, 2997
BstNSI	RCATGY	4 cut(s)	244, 1054, 2650, 2900
BstPAI	GACNNNNGTC	1 cut(s)	1812
BstPI	GGTNACC	1 cut(s)	2807
BstSFI	CTRYAG	3 cut(s)	1915, 2547, 3033
BstSLI	GKGCMC	2 cut(s)	894, 1844
BstUI	CGCG	2 cut(s)	383, 2828
BstV2I	GAAGAC	5 cut(s)	632, 1431, 1524, 2429, 2572
BstX2I	RGATCY	2 cut(s)	1193, 1346
BstXI	CCANNNNNNTGG	2 cut(s)	2930, 3134
BstYI	RGATCY	2 cut(s)	1193, 1346
BstZ17I	GTATAC	2 cut(s)	1102, 2902
Bsu15I	ATCGAT	3 cut(s)	87, 98, 2179
BsuRI	GGCC	9 cut(s)	205, 280, 2863, 2935, 3045, 3081, 3105, 3163, 3189
BsuTUI	ATCGAT	3 cut(s)	87, 98, 2179
BtgI	CCRYGG	2 cut(s)	2489, 2812
BtgZI	GCGATG	1 cut(s)	2573
BtrI	CACGTC	1 cut(s)	61
BtsI	GCAGTG	1 cut(s)	1766
BtsIMutI	CAGTG	6 cut(s)	242, 1704, 1766, 2113, 2248, 2439
BveI	ACCTGC	2 cut(s)	2393, 3175
Cac8I	GCNNGC	3 cut(s)	717, 1151, 1891
CaiI	CAGNNNCTG	1 cut(s)	3041
CfoI	GCGC	2 cut(s)	1300, 2830
Cfr10I	RCCGGY	2 cut(s)	21, 3073
Cfr13I	GGNCC	1 cut(s)	2933
Cfr9I	CCCGGG	1 cut(s)	3088
ClaI	ATCGAT	3 cut(s)	87, 98, 2179
CseI	GACGC	4 cut(s)	389, 1229, 2521, 3027
Csp6I	GTAC	5 cut(s)	40, 295, 2239, 2274, 2375
CspAI	ACCGGT	2 cut(s)	21, 3073
CviQI	GTAC	5 cut(s)	40, 295, 2239, 2274, 2375
DdeI	CTNAG	9 cut(s)	539, 929, 1707, 1908, 2055, 2679, 2871, 2946, 3111
DraI	TTTAAA	1 cut(s)	1852
EaeI	YGGCCR	4 cut(s)	3043, 3079, 3103, 3187
Eam1104I	CTCTTC	3 cut(s)	66, 1276, 1908
EarI	CTCTTC	3 cut(s)	66, 1276, 1908
EciI	GGCGGA	4 cut(s)	47, 2033, 2524, 2958
Ecl136II	GAGCTC	1 cut(s)	1226
Eco130I	CCWWGG	3 cut(s)	1810, 2489, 2753
Eco147I	AGGCCT	2 cut(s)	205, 2863
Eco24I	GRGCYC	2 cut(s)	78, 1228
Eco31I	GGTCTC	4 cut(s)	351, 2087, 2310, 2944
Eco32I	GATATC	1 cut(s)	1133
Eco53kI	GAGCTC	1 cut(s)	1226
Eco57I	CTGAAG	3 cut(s)	411, 1556, 1683
Eco88I	CYCGRG	3 cut(s)	2573, 2784, 3088
Eco91I	GGTNACC	1 cut(s)	2807
EcoICRI	GAGCTC	1 cut(s)	1226
EcoNI	CCTNNNNNAGG	2 cut(s)	2400, 2994
EcoO65I	GGTNACC	1 cut(s)	2807
EcoRI	GAATTC	2 cut(s)	957, 2887
EcoRII	CCWGG	8 cut(s)	320, 484, 556, 601, 655, 1612, 2534, 2995
EcoRV	GATATC	1 cut(s)	1133
EcoT14I	CCWWGG	3 cut(s)	1810, 2489, 2753
EcoT22I	ATGCAT	3 cut(s)	2005, 2162, 2652
EcoT38I	GRGCYC	2 cut(s)	78, 1228
ErhI	CCWWGG	3 cut(s)	1810, 2489, 2753
Esp3I	CGTCTC	1 cut(s)	2145
FalI	AAGNNNNNCTT	2 cut(s)	2031, 2063
FaqI	GGGAC	1 cut(s)	2745
FauI	CCCGC	1 cut(s)	51
FauNDI	CATATG	1 cut(s)	797
FbaI	TGATCA	2 cut(s)	1405, 2725
FblI	GTMKAC	5 cut(s)	57, 108, 1101, 1590, 2901
FriOI	GRGCYC	2 cut(s)	78, 1228
FspBI	CTAG	3 cut(s)	1496, 2051, 2754
GlaI	GCGC	2 cut(s)	1299, 2829
GsuI	CTGGAG	3 cut(s)	876, 1082, 2382
HaeII	RGCGCY	1 cut(s)	1301
HaeIII	GGCC	9 cut(s)	205, 280, 2863, 2935, 3045, 3081, 3105, 3163, 3189
HgaI	GACGC	4 cut(s)	389, 1229, 2521, 3027
HhaI	GCGC	2 cut(s)	1300, 2830
Hin1I	GRCGYC	1 cut(s)	2513
Hin6I	GCGC	2 cut(s)	1298, 2828
HinP1I	GCGC	2 cut(s)	1298, 2828
HincII	GTYRAC	5 cut(s)	58, 109, 1452, 2193, 2314
HindII	GTYRAC	5 cut(s)	58, 109, 1452, 2193, 2314
HindIII	AAGCTT	1 cut(s)	434
HpaI	GTTAAC	1 cut(s)	2193
HphI	GGTGA	9 cut(s)	170, 617, 623, 893, 1368, 1735, 1935, 2819, 2912
Hpy99I	CGWCG	4 cut(s)	62, 83, 227, 1589
HpyCH4IV	ACGT	5 cut(s)	60, 93, 1432, 1587, 1669
HpyF3I	CTNAG	9 cut(s)	539, 929, 1707, 1908, 2055, 2679, 2871, 2946, 3111
HpySE526I	ACGT	5 cut(s)	60, 93, 1432, 1587, 1669
Hsp92I	GRCGYC	1 cut(s)	2513
HspAI	GCGC	2 cut(s)	1298, 2828
Kpn2I	TCCGGA	1 cut(s)	3119
Ksp22I	TGATCA	2 cut(s)	1405, 2725
KspAI	GTTAAC	1 cut(s)	2193
LguI	GCTCTTC	2 cut(s)	66, 1908
LmnI	GCTCC	5 cut(s)	1898, 2281, 2531, 2552, 2992
MaeI	CTAG	3 cut(s)	1496, 2051, 2754
MaeII	ACGT	5 cut(s)	60, 93, 1432, 1587, 1669
MaeIII	GTNAC	6 cut(s)	1031, 2022, 2428, 2801, 2807, 3020
MbiI	CCGCTC	1 cut(s)	46
MflI	RGATCY	2 cut(s)	1193, 1346
MhlI	GDGCHC	6 cut(s)	78, 894, 1228, 1844, 1914, 2989
MlsI	TGGCCA	2 cut(s)	3045, 3189
MluNI	TGGCCA	2 cut(s)	3045, 3189
MlyI	GAGTC	4 cut(s)	446, 919, 2959, 2985
MmeI	TCCRAC	2 cut(s)	117, 1171
Mox20I	TGGCCA	2 cut(s)	3045, 3189
Mph1103I	ATGCAT	3 cut(s)	2005, 2162, 2652
MroI	TCCGGA	1 cut(s)	3119
MscI	TGGCCA	2 cut(s)	3045, 3189
MseI	TTAA	9 cut(s)	545, 573, 1160, 1277, 1569, 1599, 1851, 2192, 2601
MslI	CAYNNNNRTG	2 cut(s)	1013, 3054
Msp20I	TGGCCA	2 cut(s)	3045, 3189
MspA1I	CMGCKG	3 cut(s)	343, 389, 2582
Mva1269I	GAATGC	2 cut(s)	2650, 2748
MvaI	CCWGG	8 cut(s)	322, 486, 558, 603, 657, 1614, 2536, 2997
MvnI	CGCG	2 cut(s)	383, 2828
NciI	CCSGG	6 cut(s)	774, 2098, 2479, 2973, 3089, 3090
NcoI	CCATGG	1 cut(s)	2489
NdeI	CATATG	1 cut(s)	797
NlaIV	GGNNCC	3 cut(s)	1326, 2283, 3069
NmeAIII	GCCGAG	3 cut(s)	328, 1504, 2698
NmuCI	GTSAC	2 cut(s)	2801, 2807
NsiI	ATGCAT	3 cut(s)	2005, 2162, 2652
NspI	RCATGY	4 cut(s)	244, 1054, 2650, 2900
NspV	TTCGAA	1 cut(s)	2792
PaqCI	CACCTGC	2 cut(s)	2393, 3175
PasI	CCCWGGG	1 cut(s)	321
PceI	AGGCCT	2 cut(s)	205, 2863
PciSI	GCTCTTC	2 cut(s)	66, 1908
PcsI	WCGNNNNNNNCGW	2 cut(s)	54, 1032
PctI	GAATGC	2 cut(s)	2650, 2748
PfeI	GAWTC	8 cut(s)	100, 334, 467, 1066, 1316, 1543, 2624, 2909
PflMI	CCANNNNNTGG	1 cut(s)	2276
PfoI	TCCNGGA	1 cut(s)	484
PinAI	ACCGGT	2 cut(s)	21, 3073
PleI	GAGTC	4 cut(s)	446, 919, 2959, 2984
PpsI	GAGTC	4 cut(s)	446, 919, 2959, 2984
PshAI	GACNNNNGTC	1 cut(s)	1812
Psp124BI	GAGCTC	1 cut(s)	1228
Psp6I	CCWGG	8 cut(s)	320, 484, 556, 601, 655, 1612, 2534, 2995
PspEI	GGTNACC	1 cut(s)	2807
PspGI	CCWGG	8 cut(s)	320, 484, 556, 601, 655, 1612, 2534, 2995
PspN4I	GGNNCC	3 cut(s)	1326, 2283, 3069
PspPI	GGNCC	1 cut(s)	2933
PstI	CTGCAG	2 cut(s)	2551, 3037
PstNI	CAGNNNCTG	1 cut(s)	3041
PsuI	RGATCY	2 cut(s)	1193, 1346
PvuII	CAGCTG	1 cut(s)	389
RsaI	GTAC	5 cut(s)	41, 296, 2240, 2275, 2376
RsaNI	GTAC	5 cut(s)	40, 295, 2239, 2274, 2375
RseI	CAYNNNNRTG	2 cut(s)	1013, 3054
SacI	GAGCTC	1 cut(s)	1228
SalI	GTCGAC	2 cut(s)	56, 107
SapI	GCTCTTC	2 cut(s)	66, 1908
SaqAI	TTAA	9 cut(s)	545, 573, 1160, 1277, 1569, 1599, 1851, 2192, 2601
Sau96I	GGNCC	1 cut(s)	2933
SbfI	CCTGCAGG	1 cut(s)	2551
SchI	GAGTC	4 cut(s)	446, 919, 2959, 2985
SdaI	CCTGCAGG	1 cut(s)	2551
SduI	GDGCHC	6 cut(s)	78, 894, 1228, 1844, 1914, 2989
SfcI	CTRYAG	3 cut(s)	1915, 2547, 3033
SfuI	TTCGAA	1 cut(s)	2792
SmaI	CCCGGG	1 cut(s)	3090
SmiMI	CAYNNNNRTG	2 cut(s)	1013, 3054
Sse8387I	CCTGCAGG	1 cut(s)	2551
SseBI	AGGCCT	2 cut(s)	205, 2863
SspI	AATATT	3 cut(s)	938, 1212, 2797
SspMI	CTAG	3 cut(s)	1496, 2051, 2754
SstI	GAGCTC	1 cut(s)	1228
StuI	AGGCCT	2 cut(s)	205, 2863
StyI	CCWWGG	3 cut(s)	1810, 2489, 2753
TaiI	ACGT	5 cut(s)	63, 96, 1435, 1590, 1672
TatI	WGTACW	4 cut(s)	294, 2238, 2273, 2374
TauI	GCSGC	3 cut(s)	386, 2585, 3108
TfiI	GAWTC	8 cut(s)	100, 334, 467, 1066, 1316, 1543, 2624, 2909
Tru1I	TTAA	9 cut(s)	545, 573, 1160, 1277, 1569, 1599, 1851, 2192, 2601
Tru9I	TTAA	9 cut(s)	545, 573, 1160, 1277, 1569, 1599, 1851, 2192, 2601
TscAI	CASTG	6 cut(s)	242, 1711, 1766, 2113, 2248, 2439
TseFI	GTSAC	2 cut(s)	2801, 2807
Tsp45I	GTSAC	2 cut(s)	2801, 2807
TspGWI	ACGGA	5 cut(s)	862, 2099, 2239, 2636, 2730
TspMI	CCCGGG	1 cut(s)	3088
TspRI	CASTG	6 cut(s)	242, 1711, 1766, 2113, 2248, 2439
Van91I	CCANNNNNTGG	1 cut(s)	2276
XagI	CCTNNNNNAGG	2 cut(s)	2400, 2994
XapI	RAATTY	7 cut(s)	494, 957, 1112, 1156, 1939, 2500, 2887
XceI	RCATGY	4 cut(s)	244, 1054, 2650, 2900
XcmI	CCANNNNNNNNNTGG	2 cut(s)	686, 3182
XmaI	CCCGGG	1 cut(s)	3088
XmaJI	CCTAGG	1 cut(s)	2753
XmiI	GTMKAC	5 cut(s)	57, 108, 1101, 1590, 2901
XspI	CTAG	3 cut(s)	1496, 2051, 2754
Zsp2I	ATGCAT	3 cut(s)	2005, 2162, 2652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.