Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002185

Physical Location & Seq

Genomic Coordinates Reverse (-)

777156 .. 785540

Gene Structure 8385 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1014000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3210 bp

ATGGGAGTTCCGGCGTTCTACCGGTGGTTGGCGGAGAAGTACCCGCTTGTGATTGTCGACGTCGTGGAAGAAGAGCCCGTCGTGATAGATGACGTATCGATTCCGGTCGACACCAGTAAACCAAACCCTAATGGGTTGGAATACGATAATCTGTATCTCGACATGAACGGGATTATTCACCCCTGTTTCCACCCAGAAGATAGGCCTTCTCCCTCGACATTCGACGAGGTATTCCAGTGCATGTTTGATTATATTGACAGACTGTTTGTGATGGTTCGGCCTCGAAAGCTATTGTACATGGCTATTGATGGTGTTGCCCCCAGGGCAAAAATGAATCAACAGCGGTCTCGGCGATTTAGAGCTGCGAAAGATGCATCAGACGCGGCAGCTGAAGAAGAAAGGCTGCGTGAGGAGTTTGAAATGGAGGGGAGAAAACTTCCTCCAAAACAGCAGTCGCAAGTTGTTGATTCCAATGTCATCACTCCTGGAACTCAATTTATGGCTGTTTTGTCAGTAGCATTGCAGTATTACATTCATCTCAGATTAAACAACGACCCAGGATGGAAAAATGTCAAGGTTATTCTTTCCGATGCAAATGTTCCAGGTGAAGGTGAACACAAAATTATGTCTTATGTTCGTCTTCAAAGAAATCTTCCTGGTTATGATCCAAATACACGCCATTGTCTCTATGGTTTGGATGCTGATTTGATTATGCTTGCTTTGGCTACCCATGAAGTTCATTTTTCAATTCTTCGAGAGGTTGTATTCACCCCCGGACAGCAAGACAAATGCTTCATATGTGGTCAGGTGGGTCATTTAGCAGCAAATTGTGAAGGTAAGGCGAAACGGAAGGCTGGAGAGTTTGATGAGAAAGCTGATGCTGAGGCCATTCCCAAGAAGCCGTACCAGTTTCTTAACATCTGGACTCTTAGAGAATATTTGGAATATGAGATGAGAATTCCCAATACCCAATTCGAGATTGATTTTGAGTGCATTGTGGATGATTTCATATTCATGTGTTTCTTTGTTGGCAACGATTTCTTACCACACATGCCCACACTGGAAATTCGTGAGGGTGCAATCAATTTGCTTTTGGCTGTATACAAGAAAGAATTTAAGTCATTGGGTGGATACCTGACTGATGGAAGCAAGCCAAATTTGAGTCGGGTAGAGCATTTCATCCAAGCAGTTGGATCTTATGAAGATAAAATATTTCAGAAAAGAGCTCGATTGCATCAGCGTCAGGCAGACAGAATAAAGCGTGAAAAAGGGCAATTAAGAAGAGGAGATGATGCAGCGCCTCAAGTGCAACCAGATTCATTGGTTCCAGTTTCTCGGTTTCAGGGATCTCGTCTTGCTTCAGGTCATACAGCTTCACCCTATCAACAATCTGGAAAATCTAGTGATCAAGGAAATCCTCGAAAGGTTGCACGTTTGTCTTCAGAGATGTCAACTGTCGGTGCTGCTATTGTTGAAGCTGAGGACAGTCTTGAACTAGATGAACATGGAAACAAAGAAGACATGAAAGCAAAGCTGAAGGAGATTCTTCGTGAGAAATCTGATGCTTTTAACTCTGGAAATCCCAACGTAGACAAGGTTAAATTGGGAGAACCTGGATGGAAAGATAGGTATTATGAAGAGAAGTTTTCTGCAAAAACTCCTGAAGAACGTGATGCAATTCGAAAAGATGTTGTTTTGCGATACACTGAAGGTCTGTGTTGGGTGATGCACTATTATTATGAAGGTGTTTGCTCGTGGCAGTGGTTTTACCCCTATCACTATGCACCCTTTGCTTCTGATCTGAAGGACCTTGGTCAGCTAAATATAAGTTTCGATTTGGGCTCTCCTTTTAAACCGTTCAATCAGCTATTGGGAGTTTTTCCTGCTGCAAGCTCCCATGCTCTTCCTGAGCAGTACAGGAAGTTGATGACTGATCCAAATTCACCTCTGATTGATTTTTACCCTATTGACTTTGAAGTGGATATGAATGGGAAGCGGTATGCATGGCAGGGTATTGCAAAGCTGCCTTTTATTGATGAAGACCGCCTTCTATGTGAGGTTGAAAAACTCGAACATACATTGACGGAGGAGGAGACAAGGAGAAATAGTGTGATGTTCGAGATGCTTTTTGTGACATCGTCTCATCCTCTATCTGTAAGCATATATTCTCTGGACGATCGATGCAAGCAGTTGACAGACAAAGAACGAGTTGAAGTTAAGGAGCAGATTGATCCCAAGTACAGGGATGCATGCAGTGAAGGGATGAACGGTTATTTGTCTCCATGTACTGGAGAGCCTTGCCCTCCGGTTTTCCGGTCTCCTGTTGAAAGCATGGAGGATATTGTGGACAACCAAGTAATATGTGCGATATACAGACTACCTGATGTACATAAGCATATAACTCGACCACCGGCAGGTGTTATATTCCCGAAGAAGACTGTTACAGTCGGGGATCTGAAGCCATTACCTCCTTTATGGCATGAAGACACCGGGCGAAAGCCTTGGCATGAAGATTCTGGGCGGAAGCCATATGAAAATTCGAGTTCCAGGCAGAACCCAGGAGCTATTTCTGGAAGACAGCTCGGGGAATCGGCACATCGCCTTGTTGTTAATAGCTTACAGGCTAATGGGTATGGCAATCAGATGCATTCCCATCGACCATCTTATGCCGAGCCTCAGTATTATCCAGCACACCCTTCCTATGCAAATAACAGAGGCTATGATCAGGGACACAATAGAATGCCACCACCTAGGGCAATCCACCATCCTCAACATCAACACCGAGCTTCGAATATTAGTACATTCCCAAGTCACGGTGACCGTGGGTATAATGTACCGCTTTCTTCGGCAGCTGGCCATCGCTTTCAGAACAGGTCTCGTACCCAGCACCACGAAAGGAATTCTGGGCATGTATACTACGAATCAGGAAATCACCAGAACGATGGGCCAATCTACCCTGAGAGACCAATTCTACAAACTCCACCGGGGGTCAGTGCTGCTCCTTATCCAGGTGGCTATAACAACTATCAGAGTTACCAACCATCTGGAACGCCTAGCCATCATGGTATTGATGGCCGGGTTCCACCGGTCAGCCATAACCCGGGCAGAGGTCATAGCCGTCCTCAACAATCCAGAAACCAATACTCTGGATTGGACAGAAGAGGAAATAGGAGACCACCTCCACCCCAGGCTGGGTATGGCCACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000966 GO:0000967 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004534 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008409 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0022613 GO:0034470 GO:0034471 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0071025 GO:0071027 GO:0071029 GO:0071035 GO:0071046 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03008 ko03018 map03008 map03018

Pfam Domains

Protein Families

XRN_N zf-CCHC

Protein Analysis

1070

Amino Acids

121.72

Weight (kDa)

6.52

Isoelectric Point (pI)

49.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XRN_N	PF03159	1 - 253	2.7e-101	XRN 5'-3' exonuclease N-terminus
XRN_M	PF17846	327 - 818	6e-164	Xrn1 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002185)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75660 AT5G42540 AT5G42540
fragaria_vesca	FvH4_2g38700 FvH4_2g38700 FvH4_5g21470
malus_domestica	MD00G1128500.v1.1 MD08G1014700.v1.1 MD15G1014000.v1.1
prunus_persica	Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1
pyrus_communis	pycom08g01310 pycom15g01210
rosa_chinensis	RchiOBHm_Chr5g0009961 RchiOBHm_Chr6g0302611
rosa_laevigata	RLG00000011157 RLG00000031714
rosa_multiflora	Rmu_sc0027322.1_g000001 Rmu_sc0027323.1_g000001 Rmu_sc0039467.1_g000001 Rmu_sc0039989.1_g000001 Rmu_sc0041627.1_g000001
rosa_roxburghii	Rroxscaffold_1G00066230 Rroxscaffold_7G00165520
rosa_rugosa	Rorug04G0447300 Rorug06G0315300 Rorug06G0315400
rosa_samantha	Rh5AG078800 Rh5BG073500 Rh5CG086100 Rh5DG074200 Rh5DG336700 Rh6AG428700 Rh6BG473800 Rh6CG441000 Rh6DG427700
rosa_wichuraiana	Rw6G037100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2405
AatII	GACGTC	1 cut(s)	63
Acc36I	ACCTGC	1 cut(s)	2405
AccB7I	CCANNNNNTGG	1 cut(s)	2288
AccI	GTMKAC	5 cut(s)	57, 108, 1101, 1590, 2913
AccII	CGCG	1 cut(s)	383
AciI	CCGC	8 cut(s)	32, 44, 343, 383, 1996, 2044, 2521, 2837
AclWI	GGATC	6 cut(s)	659, 1201, 1354, 1928, 2225, 2460
AcoI	YGGCCR	3 cut(s)	2854, 3073, 3199
AcsI	RAATTY	7 cut(s)	957, 1065, 1112, 1156, 1939, 2536, 2899
AcuI	CTGAAG	8 cut(s)	411, 1344, 1425, 1556, 1683, 1728, 1823, 2477
AcyI	GRCGYC	1 cut(s)	60
AgeI	ACCGGT	2 cut(s)	21, 3085
AjuI	GAANNNNNNNTTGG	2 cut(s)	956, 988
Alw21I	GWGCWC	1 cut(s)	1228
Alw26I	GTCTC	9 cut(s)	351, 689, 2087, 2145, 2283, 2322, 2880, 2956, 3166
AlwI	GGATC	6 cut(s)	659, 1201, 1354, 1928, 2225, 2460
Ama87I	CYCGRG	2 cut(s)	2582, 3100
AoxI	GGCC	7 cut(s)	203, 278, 885, 2854, 2945, 3073, 3199
ApoI	RAATTY	7 cut(s)	957, 1065, 1112, 1156, 1939, 2536, 2899
ArsI	GACNNNNNNTTYG	4 cut(s)	248, 280, 437, 469
AsiGI	ACCGGT	2 cut(s)	21, 3085
AspA2I	CCTAGG	1 cut(s)	2750
AspLEI	GCGC	1 cut(s)	1300
AspS9I	GGNCC	2 cut(s)	1807, 2945
AsuC2I	CCSGG	6 cut(s)	774, 2491, 2985, 3077, 3101, 3102
AsuHPI	GGTGA	9 cut(s)	170, 617, 623, 760, 1368, 1735, 1935, 2828, 2924
AsuII	TTCGAA	2 cut(s)	1681, 2789
AvaI	CYCGRG	2 cut(s)	2582, 3100
AvaII	GGWCC	1 cut(s)	1807
AvrII	CCTAGG	1 cut(s)	2750
BalI	TGGCCA	2 cut(s)	2856, 3201
BanII	GRGCYC	3 cut(s)	78, 1228, 1844
BauI	CACGAG	1 cut(s)	1753
BbsI	GAAGAC	7 cut(s)	632, 1431, 1524, 2046, 2441, 2490, 2581
Bbv12I	GWGCWC	1 cut(s)	1228
BbvCI	CCTCAGC	2 cut(s)	882, 1479
BceAI	ACGGC	2 cut(s)	886, 3102
BcgI	CGANNNNNNTGC	4 cut(s)	2384, 2418, 2636, 2670
BciVI	GTATCC	1 cut(s)	1124
BclI	TGATCA	2 cut(s)	1405, 2722
BcnI	CCSGG	6 cut(s)	774, 2491, 2985, 3077, 3101, 3102
BcoDI	GTCTC	9 cut(s)	351, 689, 2087, 2145, 2283, 2322, 2880, 2956, 3166
BfaI	CTAG	4 cut(s)	1401, 1496, 2751, 3054
BfoI	RGCGCY	1 cut(s)	1301
BfuAI	ACCTGC	1 cut(s)	2405
BfuI	GTATCC	1 cut(s)	1124
BglI	GCCNNNNNGGC	1 cut(s)	323
BlnI	CCTAGG	1 cut(s)	2750
Bme18I	GGWCC	1 cut(s)	1807
BmeT110I	CYCGRG	2 cut(s)	2582, 3100
BmgT120I	GGNCC	2 cut(s)	1807, 2945
BmiI	GGNNCC	2 cut(s)	1326, 3081
BoxI	GACNNNNGTC	1 cut(s)	1812
BpiI	GAAGAC	7 cut(s)	632, 1431, 1524, 2046, 2441, 2490, 2581
BplI	GAGNNNNNCTC	2 cut(s)	3163, 3195
BpmI	CTGGAG	2 cut(s)	876, 2310
Bpu10I	CCTNAGC	3 cut(s)	882, 1479, 1908
Bpu14I	TTCGAA	2 cut(s)	1681, 2789
BpuEI	CTTGAG	1 cut(s)	1287
BpuMI	CCSGG	6 cut(s)	774, 2491, 2985, 3077, 3101, 3102
Bsa29I	ATCGAT	2 cut(s)	98, 2179
BsaHI	GRCGYC	1 cut(s)	60
BsaI	GGTCTC	5 cut(s)	351, 2322, 2880, 2956, 3166
BsaWI	WCCGGW	5 cut(s)	21, 103, 2305, 2313, 3085
BsaXI	ACNNNNNCTCC	2 cut(s)	2092, 2122
Bse118I	RCCGGY	3 cut(s)	21, 2410, 3085
Bse1I	ACTGG	6 cut(s)	114, 235, 907, 1065, 1328, 2293
Bse3DI	GCAATG	1 cut(s)	518
BseCI	ATCGAT	2 cut(s)	98, 2179
BseGI	GGATG	9 cut(s)	566, 703, 1006, 1179, 1622, 2143, 2251, 2268, 2764
BseMI	GCAATG	1 cut(s)	518
BseMII	CTCAG	6 cut(s)	553, 873, 1470, 1899, 2690, 2949
BseNI	ACTGG	6 cut(s)	114, 235, 907, 1065, 1328, 2293
BseRI	GAGGAG	4 cut(s)	425, 1299, 2102, 2105
BseYI	CCCAGC	2 cut(s)	2883, 3191
Bsh1236I	CGCG	1 cut(s)	383
Bsh1285I	CGRYCG	2 cut(s)	108, 2179
BshFI	GGCC	7 cut(s)	205, 280, 887, 2856, 2947, 3075, 3201
BshTI	ACCGGT	2 cut(s)	21, 3085
BshVI	ATCGAT	2 cut(s)	98, 2179
BsiEI	CGRYCG	2 cut(s)	108, 2179
BsiHKAI	GWGCWC	1 cut(s)	1228
BsiHKCI	CYCGRG	2 cut(s)	2582, 3100
BslFI	GGGAC	1 cut(s)	2742
BsmAI	GTCTC	9 cut(s)	351, 689, 2087, 2145, 2283, 2322, 2880, 2956, 3166
BsmBI	CGTCTC	1 cut(s)	2145
BsmFI	GGGAC	1 cut(s)	2742
BsmI	GAATGC	2 cut(s)	2647, 2745
BsnI	GGCC	7 cut(s)	205, 280, 887, 2856, 2947, 3075, 3201
Bso31I	GGTCTC	5 cut(s)	351, 2322, 2880, 2956, 3166
BsoBI	CYCGRG	2 cut(s)	2582, 3100
Bsp119I	TTCGAA	2 cut(s)	1681, 2789
Bsp1286I	GDGCHC	3 cut(s)	78, 1228, 1844
Bsp1407I	TGTACA	2 cut(s)	294, 2386
BspACI	CCGC	8 cut(s)	32, 44, 343, 383, 1996, 2044, 2521, 2837
BspANI	GGCC	7 cut(s)	205, 280, 887, 2856, 2947, 3075, 3201
BspCNI	CTCAG	6 cut(s)	552, 874, 1471, 1900, 2689, 2950
BspDI	ATCGAT	2 cut(s)	98, 2179
BspFNI	CGCG	1 cut(s)	383
BspLI	GGNNCC	2 cut(s)	1326, 3081
BspMI	ACCTGC	1 cut(s)	2405
BspPI	GGATC	6 cut(s)	659, 1201, 1354, 1928, 2225, 2460
BspQI	GCTCTTC	2 cut(s)	66, 1908
BspT104I	TTCGAA	2 cut(s)	1681, 2789
BspTNI	GGTCTC	5 cut(s)	351, 2322, 2880, 2956, 3166
BsrDI	GCAATG	1 cut(s)	518
BsrFI	RCCGGY	3 cut(s)	21, 2410, 3085
BsrGI	TGTACA	2 cut(s)	294, 2386
BsrI	ACTGG	6 cut(s)	114, 235, 907, 1065, 1328, 2293
BssAI	RCCGGY	3 cut(s)	21, 2410, 3085
BssNAI	GTATAC	2 cut(s)	1102, 2914
BssNI	GRCGYC	1 cut(s)	60
BssSI	CACGAG	1 cut(s)	1753
BssT1I	CCWWGG	3 cut(s)	1810, 2501, 2750
Bst1107I	GTATAC	2 cut(s)	1102, 2914
Bst2BI	CACGAG	1 cut(s)	1753
Bst4CI	ACNGT	9 cut(s)	264, 1456, 1487, 1857, 2270, 2440, 2446, 2816, 2822
Bst6I	CTCTTC	5 cut(s)	66, 1276, 1632, 1908, 3154
BstACI	GRCGYC	1 cut(s)	60
BstAPI	GCANNNNNTGC	1 cut(s)	1790
BstAUI	TGTACA	2 cut(s)	294, 2386
BstBI	TTCGAA	2 cut(s)	1681, 2789
BstC8I	GCNNGC	6 cut(s)	717, 1151, 1891, 2186, 2251, 2854
BstDEI	CTNAG	7 cut(s)	539, 882, 929, 1479, 1908, 2676, 2958
BstDSI	CCRYGG	1 cut(s)	2821
BstEII	GGTNACC	1 cut(s)	2816
BstENI	CCTNNNNNAGG	1 cut(s)	3006
BstF5I	GGATG	9 cut(s)	566, 703, 1006, 1179, 1622, 2143, 2251, 2268, 2764
BstFNI	CGCG	1 cut(s)	383
BstH2I	RGCGCY	1 cut(s)	1301
BstHHI	GCGC	1 cut(s)	1300
BstMAI	GTCTC	9 cut(s)	351, 689, 2087, 2145, 2283, 2322, 2880, 2956, 3166
BstMCI	CGRYCG	2 cut(s)	108, 2179
BstMWI	GCNNNNNNNGC	7 cut(s)	286, 323, 349, 371, 380, 1306, 1790
BstNSI	RCATGY	4 cut(s)	244, 1054, 2253, 2912
BstPAI	GACNNNNGTC	1 cut(s)	1812
BstPI	GGTNACC	1 cut(s)	2816
BstUI	CGCG	1 cut(s)	383
BstV2I	GAAGAC	7 cut(s)	632, 1431, 1524, 2046, 2441, 2490, 2581
BstX2I	RGATCY	3 cut(s)	1193, 1346, 2452
BstXI	CCANNNNNNTGG	4 cut(s)	1190, 2942, 3044, 3146
BstYI	RGATCY	3 cut(s)	1193, 1346, 2452
BstZ17I	GTATAC	2 cut(s)	1102, 2914
Bsu15I	ATCGAT	2 cut(s)	98, 2179
BsuI	GTATCC	1 cut(s)	1124
BsuRI	GGCC	7 cut(s)	205, 280, 887, 2856, 2947, 3075, 3201
BsuTUI	ATCGAT	2 cut(s)	98, 2179
BtgI	CCRYGG	1 cut(s)	2821
BtgZI	GCGATG	2 cut(s)	2582, 2843
BtsCI	GGATG	9 cut(s)	566, 703, 1006, 1179, 1622, 2143, 2251, 2268, 2764
BtsI	GCAGTG	2 cut(s)	1766, 2260
BtsIMutI	CAGTG	6 cut(s)	242, 1058, 1704, 1766, 2260, 2998
BveI	ACCTGC	1 cut(s)	2405
Cac8I	GCNNGC	6 cut(s)	717, 1151, 1891, 2186, 2251, 2854
CfoI	GCGC	1 cut(s)	1300
Cfr10I	RCCGGY	3 cut(s)	21, 2410, 3085
Cfr13I	GGNCC	2 cut(s)	1807, 2945
Cfr9I	CCCGGG	1 cut(s)	3100
ClaI	ATCGAT	2 cut(s)	98, 2179
CseI	GACGC	2 cut(s)	389, 1229
CspAI	ACCGGT	2 cut(s)	21, 3085
DdeI	CTNAG	7 cut(s)	539, 882, 929, 1479, 1908, 2676, 2958
DraI	TTTAAA	1 cut(s)	1852
EaeI	YGGCCR	3 cut(s)	2854, 3073, 3199
Eam1104I	CTCTTC	5 cut(s)	66, 1276, 1632, 1908, 3154
EarI	CTCTTC	5 cut(s)	66, 1276, 1632, 1908, 3154
EciI	GGCGGA	2 cut(s)	47, 2536
Ecl136II	GAGCTC	1 cut(s)	1226
Eco130I	CCWWGG	3 cut(s)	1810, 2501, 2750
Eco147I	AGGCCT	1 cut(s)	205
Eco24I	GRGCYC	3 cut(s)	78, 1228, 1844
Eco31I	GGTCTC	5 cut(s)	351, 2322, 2880, 2956, 3166
Eco47I	GGWCC	1 cut(s)	1807
Eco53kI	GAGCTC	1 cut(s)	1226
Eco57I	CTGAAG	8 cut(s)	411, 1344, 1425, 1556, 1683, 1728, 1823, 2477
Eco88I	CYCGRG	2 cut(s)	2582, 3100
Eco91I	GGTNACC	1 cut(s)	2816
EcoICRI	GAGCTC	1 cut(s)	1226
EcoNI	CCTNNNNNAGG	1 cut(s)	3006
EcoO109I	RGGNCCY	1 cut(s)	1807
EcoO65I	GGTNACC	1 cut(s)	2816
EcoRI	GAATTC	2 cut(s)	957, 2899
EcoT14I	CCWWGG	3 cut(s)	1810, 2501, 2750
EcoT22I	ATGCAT	4 cut(s)	376, 2005, 2251, 2649
EcoT38I	GRGCYC	3 cut(s)	78, 1228, 1844
ErhI	CCWWGG	3 cut(s)	1810, 2501, 2750
Esp3I	CGTCTC	1 cut(s)	2145
FalI	AAGNNNNNCTT	2 cut(s)	2031, 2063
FaqI	GGGAC	1 cut(s)	2742
FauI	CCCGC	1 cut(s)	51
FauNDI	CATATG	2 cut(s)	797, 2530
FbaI	TGATCA	2 cut(s)	1405, 2722
FblI	GTMKAC	5 cut(s)	57, 108, 1101, 1590, 2913
FokI	GGATG	9 cut(s)	573, 710, 1013, 1166, 1629, 2130, 2258, 2275, 2751
FriOI	GRGCYC	3 cut(s)	78, 1228, 1844
FspBI	CTAG	4 cut(s)	1401, 1496, 2751, 3054
GlaI	GCGC	1 cut(s)	1299
GsaI	CCCAGC	2 cut(s)	2887, 3195
GsuI	CTGGAG	2 cut(s)	876, 2310
HaeII	RGCGCY	1 cut(s)	1301
HaeIII	GGCC	7 cut(s)	205, 280, 887, 2856, 2947, 3075, 3201
HgaI	GACGC	2 cut(s)	389, 1229
HhaI	GCGC	1 cut(s)	1300
Hin1I	GRCGYC	1 cut(s)	60
Hin6I	GCGC	1 cut(s)	1298
HinP1I	GCGC	1 cut(s)	1298
HincII	GTYRAC	4 cut(s)	58, 109, 1452, 2193
HindII	GTYRAC	4 cut(s)	58, 109, 1452, 2193
HphI	GGTGA	9 cut(s)	170, 617, 623, 760, 1368, 1735, 1935, 2828, 2924
Hpy99I	CGWCG	4 cut(s)	62, 65, 83, 227
HpyCH4III	ACNGT	9 cut(s)	264, 1456, 1487, 1857, 2270, 2440, 2446, 2816, 2822
HpyCH4IV	ACGT	5 cut(s)	60, 93, 1432, 1587, 1669
HpyF10VI	GCNNNNNNNGC	7 cut(s)	286, 323, 349, 371, 380, 1306, 1790
HpyF3I	CTNAG	7 cut(s)	539, 882, 929, 1479, 1908, 2676, 2958
HpySE526I	ACGT	5 cut(s)	60, 93, 1432, 1587, 1669
Hsp92I	GRCGYC	1 cut(s)	60
HspAI	GCGC	1 cut(s)	1298
Ksp22I	TGATCA	2 cut(s)	1405, 2722
LguI	GCTCTTC	2 cut(s)	66, 1908
LmnI	GCTCC	4 cut(s)	1898, 2221, 2561, 3004
MaeI	CTAG	4 cut(s)	1401, 1496, 2751, 3054
MaeII	ACGT	5 cut(s)	60, 93, 1432, 1587, 1669
MaeIII	GTNAC	5 cut(s)	2131, 2440, 2810, 2816, 3032
MflI	RGATCY	3 cut(s)	1193, 1346, 2452
MhlI	GDGCHC	3 cut(s)	78, 1228, 1844
MlsI	TGGCCA	2 cut(s)	2856, 3201
MluNI	TGGCCA	2 cut(s)	2856, 3201
MlyI	GAGTC	2 cut(s)	919, 1171
MmeI	TCCRAC	2 cut(s)	117, 1171
Mox20I	TGGCCA	2 cut(s)	2856, 3201
Mph1103I	ATGCAT	4 cut(s)	376, 2005, 2251, 2649
MscI	TGGCCA	2 cut(s)	2856, 3201
MseI	TTAA	9 cut(s)	545, 915, 1116, 1277, 1569, 1599, 1851, 2217, 2610
MslI	CAYNNNNRTG	1 cut(s)	1013
Msp20I	TGGCCA	2 cut(s)	2856, 3201
MspA1I	CMGCKG	3 cut(s)	343, 389, 2852
Mva1269I	GAATGC	2 cut(s)	2647, 2745
MvnI	CGCG	1 cut(s)	383
MwoI	GCNNNNNNNGC	7 cut(s)	286, 323, 349, 371, 380, 1306, 1790
NciI	CCSGG	6 cut(s)	774, 2491, 2985, 3077, 3101, 3102
NdeI	CATATG	2 cut(s)	797, 2530
NlaIV	GGNNCC	2 cut(s)	1326, 3081
NmeAIII	GCCGAG	2 cut(s)	328, 2695
NmuCI	GTSAC	3 cut(s)	2131, 2810, 2816
NsiI	ATGCAT	4 cut(s)	376, 2005, 2251, 2649
NspI	RCATGY	4 cut(s)	244, 1054, 2253, 2912
NspV	TTCGAA	2 cut(s)	1681, 2789
PaeI	GCATGC	1 cut(s)	2253
PaqCI	CACCTGC	1 cut(s)	2405
PasI	CCCWGGG	1 cut(s)	321
PceI	AGGCCT	1 cut(s)	205
PciSI	GCTCTTC	2 cut(s)	66, 1908
PctI	GAATGC	2 cut(s)	2647, 2745
PfeI	GAWTC	8 cut(s)	100, 334, 467, 1316, 1543, 2513, 2588, 2921
PflFI	GACNNNGTC	1 cut(s)	2137
PflMI	CCANNNNNTGG	1 cut(s)	2288
PfoI	TCCNGGA	1 cut(s)	484
PinAI	ACCGGT	2 cut(s)	21, 3085
Ple19I	CGATCG	1 cut(s)	2179
PleI	GAGTC	2 cut(s)	919, 1170
PpsI	GAGTC	2 cut(s)	919, 1170
PpuMI	RGGWCCY	1 cut(s)	1807
PshAI	GACNNNNGTC	1 cut(s)	1812
Psp124BI	GAGCTC	1 cut(s)	1228
Psp5II	RGGWCCY	1 cut(s)	1807
PspEI	GGTNACC	1 cut(s)	2816
PspFI	CCCAGC	2 cut(s)	2883, 3191
PspN4I	GGNNCC	2 cut(s)	1326, 3081
PspPI	GGNCC	2 cut(s)	1807, 2945
PspPPI	RGGWCCY	1 cut(s)	1807
PsuI	RGATCY	3 cut(s)	1193, 1346, 2452
PsyI	GACNNNGTC	1 cut(s)	2137
PvuI	CGATCG	1 cut(s)	2179
PvuII	CAGCTG	2 cut(s)	389, 2852
RseI	CAYNNNNRTG	1 cut(s)	1013
SacI	GAGCTC	1 cut(s)	1228
SalI	GTCGAC	2 cut(s)	56, 107
SapI	GCTCTTC	2 cut(s)	66, 1908
SaqAI	TTAA	9 cut(s)	545, 915, 1116, 1277, 1569, 1599, 1851, 2217, 2610
Sau96I	GGNCC	2 cut(s)	1807, 2945
SchI	GAGTC	2 cut(s)	919, 1171
SduI	GDGCHC	3 cut(s)	78, 1228, 1844
SfuI	TTCGAA	2 cut(s)	1681, 2789
SinI	GGWCC	1 cut(s)	1807
SmaI	CCCGGG	1 cut(s)	3102
SmiMI	CAYNNNNRTG	1 cut(s)	1013
SmlI	CTYRAG	1 cut(s)	1302
SmoI	CTYRAG	1 cut(s)	1302
SphI	GCATGC	1 cut(s)	2253
SseBI	AGGCCT	1 cut(s)	205
SsiI	CCGC	8 cut(s)	32, 44, 343, 383, 1996, 2044, 2521, 2837
SspI	AATATT	3 cut(s)	938, 1212, 2794
SspMI	CTAG	4 cut(s)	1401, 1496, 2751, 3054
SstI	GAGCTC	1 cut(s)	1228
StuI	AGGCCT	1 cut(s)	205
StyI	CCWWGG	3 cut(s)	1810, 2501, 2750
TaaI	ACNGT	9 cut(s)	264, 1456, 1487, 1857, 2270, 2440, 2446, 2816, 2822
TaiI	ACGT	5 cut(s)	63, 96, 1435, 1590, 1672
TatI	WGTACW	6 cut(s)	294, 1914, 2238, 2285, 2386, 2798
TauI	GCSGC	1 cut(s)	386
TfiI	GAWTC	8 cut(s)	100, 334, 467, 1316, 1543, 2513, 2588, 2921
Tru1I	TTAA	9 cut(s)	545, 915, 1116, 1277, 1569, 1599, 1851, 2217, 2610
Tru9I	TTAA	9 cut(s)	545, 915, 1116, 1277, 1569, 1599, 1851, 2217, 2610
TscAI	CASTG	6 cut(s)	242, 1065, 1711, 1766, 2260, 2998
TseFI	GTSAC	3 cut(s)	2131, 2810, 2816
Tsp45I	GTSAC	3 cut(s)	2131, 2810, 2816
TspGWI	ACGGA	2 cut(s)	862, 2099
TspMI	CCCGGG	1 cut(s)	3100
TspRI	CASTG	6 cut(s)	242, 1065, 1711, 1766, 2260, 2998
Tth111I	GACNNNGTC	1 cut(s)	2137
Van91I	CCANNNNNTGG	1 cut(s)	2288
VpaK11BI	GGWCC	1 cut(s)	1807
XagI	CCTNNNNNAGG	1 cut(s)	3006
XapI	RAATTY	7 cut(s)	957, 1065, 1112, 1156, 1939, 2536, 2899
XceI	RCATGY	4 cut(s)	244, 1054, 2253, 2912
XcmI	CCANNNNNNNNNTGG	2 cut(s)	686, 3194
XmaI	CCCGGG	1 cut(s)	3100
XmaJI	CCTAGG	1 cut(s)	2750
XmiI	GTMKAC	5 cut(s)	57, 108, 1101, 1590, 2913
XspI	CTAG	4 cut(s)	1401, 1496, 2751, 3054
ZraI	GACGTC	1 cut(s)	61
Zsp2I	ATGCAT	4 cut(s)	376, 2005, 2251, 2649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.