Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002185

Physical Location & Seq

Genomic Coordinates Forward (+)

61550362 .. 61558284

Gene Structure 7923 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G037100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3129 bp

ATGGGAGTTCCAGCTTTCTACAGATGGCTAGCGGAGAAGTATCCACTCGTCGTCGTCGATGTGGTGGAAGAAGAACCGGTTGTAATCGACGAAGTTTCCATTCCGGTCGACACCAGTAAGCCAAACCCTAACGGCGAGTATGACAATCTGTACCTCGACATGAATGGGATTATTCACCCCTGTTTTCACCCTGAGGATAGGCCTTCGCCAACTACCTTTGATGAGGTATTTCAGTGCATGTTTGACTACATAGATAGGCTTTTTGTGATGGTGCGGCCTCGGAAGCTACTGTACATGGCAATTGATGGTGTTGCCCCTAGGGCGAAAATGAATCAGCAGAGATCCCGGCGGTTTAGAGCTGCTAAAGATGCAGCAGATGCGGCAGCTGAAGAAGAAAGGCTGCGTGAGGAGTTTGAAATGGAGGGAAGAAAGCTTCCACCTAAACTGCATTCGCAAACTTTTGATTCCAATGTCATTACTCCTGGAACTCCATTTATGGCTGTTTTGTCAATTGCATTGCAGTACTATATTCATCTTAGATTAAATAACGATCCTGGATGGAAAAATGTTAAGGTCATTCTTTCCGATGCAAATGTTCCGGGTGAAGGGGAGCACAAGGTTATGTCATACATTCGTCTTCAAAGAAATCTTCCTGGTTATGATCCAAATACACGTCATTGCCTGTATGGTTTGGATGCCGATTTAATTATGCTTGCCTTGGCCACCCATGAAGTTCATTTCTCCATTCTTCGAGAGGTTGTTTTCGTCGCTGGGCAGCAGGACAAGTGCTTCCTGTGTGGTCAAGTGGGTCATTTAGCTGCCAGTTGTGAAGGAAAGGCAAAACGGAAGGCTGGAGAGCTTGATGAGAAAGGTGATGCCGAGGCTGTGGCCAAGAAGCCGTACCAGTTTCTCAATATTTGGACCCTTAGAGAATATTTGGAATATGAGATGCGGATTCCAGGTGCCCCATTTGAGATTGATATTGAGCGCATTGTGGATGACTTTATATTCCTTTGTTTCTTTGTCGGAAATGATTTCTTACCTCATATGCCTACACTGGAGATTCGGGAGGGTGCAATCAACTTGCTTTTGGCTGTATATAAAAAGGAATTTAGGGGAATGGGGGGATATCTGACTAATGCAAGCAAGCCAAATTTGAGGCGGGTGGAGCATTTCATTCAGGCAGTTGGAAGTTATGAAGATAAAATATTCCAGAAAAGAGCTCAACTGCATCTGAAGCAAGCAGAAAGAATAAAGCGTCAAAAGGCACAAGCTCGAAGAGGAGATGACGCAGAGCCCCAAGTGCAACCAGATTCTTTAGTTGCAGTTAATCGGTTTCATGGTTCTCGTCTTGCTTCAGGTCCTTCACCTGTGCCGTTTCAACAATCTGGATCTGGTAGAGGAACTCCTCAAAAGGTTGCACGCTTGTCTTCAGATGCATCAACTGTCGGTGCTGCTATTGTTGAAGCTGAGATCATTCTTGAAGATGTTCATGAAAACAAAGAAGATTTGAAAATAAAGCTCAAGGAGGTTCTTCGTGAGAAATCTGATGCTTTTAACTCTAAAAATCCTGAAGAAGACAAGGTTAAATTGGGTGAACCTGGATGGAGAGAGAGGTATTATGAAGAGAAGTTTAATGCAAAAACTCCCGAAGAACGTGAAGCAATTCGAAGAGATGTTGTTTTGCGTTACACTGAAGGTCTGTGTTGGGTGATGCACTATTATTATGAAGGGGTTTGTTCATGGCAGTGGTTTTACCCTTACCATTATGCGCCCTTTGCTTCTGATCTCAAGGACCTCGGTGAACTAGATATAAGTTTTGAATTGGGCAATCCCTTTAAACCGTTCAATCAGCTGTTAGGGGTTTTTCCTGCTGCAAGTGCCCATGCCCTTCCTGAGGCATACAGGAAGCTGATGACTGATCCAAACTCACCTCTTATTGATTTTTACCCTACTGACTTTGAAGTGGATATGAATGGGAAACGATTTGCATGGCAGGGTATTGCAAAACTCCCTTTCATTGATGAAGTCCGTCTTCTTTCTGAGGTTGAAAAACTTGAACATACATTAACGGAGGAGGAATCACGCAGAAACAGTGTGATGTTTGAGATGCTCTTTGTTACATCTTCTCATCCTCTCTCTGTGTGCATATATTCTCTTGACAATCGCTGTAAACAATTGACAGACAAAGAACGTGTTGAGTTTAAGGAACAAATTGATCCCAAATACAGGGATACATGCAGTGAAGGGATGAACGGCTATCTATCTCCATGTGCTGGAGAGCCTTGTCCGCCCGTTTTCCGATCTCCTGTTGAAAGCATGGAGGATATAATGGACAACCAAGTCATATGTGCAATATACAGACTACCAGATGTACACGAGCATATAACTCGACCACCAGCAGGGGTCATTTTCCCTAAGAAGATTGTGTCATCGGGGGATATGAAGCCTGCACCTGTTTTGTGGCATGAGGATTCAGGGAGGAAGCCATGGCATGACAATTCGGGGAGGAGGCCATATGAAAGTTCTAGACAGCACCCTCCTGGAGCCATTTCTGGAAGACAGCTTGGCGATGCCGCTCATCGACTTGTTGTTAATAGTTTACATGTTAAGGCAAACGGCAATGGGTATGGCAATCAGATGGCTGCCCATCGACCATATGCCGAACATCCATATTACCCACCACGGGCTTCCTACGCAAGTAATGGAAGCTATGATCAGGGACACCACAGTATGGCACCACCTAGAACATTCCAGCATCCTCAAGTGCAACACCAAAATTCCTACCACCCTCCGAATAGACATCATTCTCAACATTATGAAAGAAATTCTGGTCACTCGTACTCTCAGTCGGCCCGTCATCAGAATGGTGGAATTGCTTACCCCCAGAGACCAATTACACCAGCTCCACCAGGGGTCAATCCAGGTCCTTATCCAGTTGGCTATAACAACTATCAGAGTTACCAACAAGCAGGGCCGCCTGGTTATCAAGGCATTGATGCGTGGGTTCCACCGCCCCAAAATATGGGCAGAGGATATGGTCGTCCTCAGCAATCTGGAAACCCGTACTCTGGATTGGACAGGAGAGGAAATAGGAGACCACCTCCATCCCAGCATGGCCGCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000966 GO:0000967 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004534 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008409 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010604 GO:0010628 GO:0016070 GO:0016072 GO:0016075 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0022613 GO:0034470 GO:0034471 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0043633 GO:0043634 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0071025 GO:0071027 GO:0071029 GO:0071035 GO:0071046 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03008 ko03018 map03008 map03018

Pfam Domains

Protein Families

XRN_N zf-CCHC

Protein Analysis

1042

Amino Acids

118.56

Weight (kDa)

6.53

Isoelectric Point (pI)

47.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
XRN_N	PF03159	1 - 252	7.5e-102	XRN 5'-3' exonuclease N-terminus
XRN_M	PF17846	326 - 815	4.6e-164	Xrn1 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002185)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75660 AT5G42540 AT5G42540
fragaria_vesca	FvH4_2g38700 FvH4_2g38700 FvH4_5g21470
malus_domestica	MD00G1128500.v1.1 MD08G1014700.v1.1 MD15G1014000.v1.1
prunus_persica	Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1 Prupe.1G367600_v2.0.a1
pyrus_communis	pycom08g01310 pycom15g01210
rosa_chinensis	RchiOBHm_Chr5g0009961 RchiOBHm_Chr6g0302611
rosa_laevigata	RLG00000011157 RLG00000031714
rosa_multiflora	Rmu_sc0027322.1_g000001 Rmu_sc0027323.1_g000001 Rmu_sc0039467.1_g000001 Rmu_sc0039989.1_g000001 Rmu_sc0041627.1_g000001
rosa_roxburghii	Rroxscaffold_1G00066230 Rroxscaffold_7G00165520
rosa_rugosa	Rorug04G0447300 Rorug06G0315300 Rorug06G0315400
rosa_samantha	Rh5AG078800 Rh5BG073500 Rh5CG086100 Rh5DG074200 Rh5DG336700 Rh6AG428700 Rh6BG473800 Rh6CG441000 Rh6DG427700
rosa_wichuraiana	Rw6G037100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2342
AccB1I	GGYRCC	2 cut(s)	962, 2737
AccB7I	CCANNNNNTGG	3 cut(s)	2276, 2734, 3025
AccBSI	CCGCTC	1 cut(s)	2579
AccI	GTMKAC	1 cut(s)	108
AclWI	GGATC	6 cut(s)	336, 545, 656, 1399, 1916, 2213
AcoI	YGGCCR	3 cut(s)	720, 888, 3118
AcsI	RAATTY	4 cut(s)	1109, 1153, 2779, 2827
AcuI	CTGAAG	6 cut(s)	408, 1256, 1341, 1416, 1593, 1716
AfaI	GTAC	7 cut(s)	152, 293, 524, 902, 2376, 2843, 3068
AfiI	CCNNNNNNNGG	9 cut(s)	605, 1897, 2229, 2276, 2402, 2686, 2734, 3025, 3071
AflIII	ACRYGT	3 cut(s)	671, 2194, 2605
AgeI	ACCGGT	1 cut(s)	76
AjiI	CACGTC	1 cut(s)	674
AjnI	CCWGG	9 cut(s)	481, 553, 652, 958, 1600, 2542, 2911, 2923, 2980
Alw21I	GWGCWC	2 cut(s)	615, 1225
Alw26I	GTCTC	2 cut(s)	2884, 3091
AlwI	GGATC	6 cut(s)	336, 545, 656, 1399, 1916, 2213
AoxI	GGCC	8 cut(s)	200, 275, 720, 888, 2513, 2853, 2975, 3118
ApeKI	GCWGC	9 cut(s)	359, 371, 383, 400, 775, 818, 1454, 1874, 2645
ApoI	RAATTY	4 cut(s)	1109, 1153, 2779, 2827
AsiGI	ACCGGT	1 cut(s)	76
Asp700I	GAANNNNTTC	2 cut(s)	1488, 1665
AspA2I	CCTAGG	1 cut(s)	317
AspLEI	GCGC	2 cut(s)	990, 1774
AspS9I	GGNCC	6 cut(s)	921, 1361, 1795, 2854, 2927, 2975
AsuC2I	CCSGG	2 cut(s)	346, 600
AsuHPI	GGTGA	9 cut(s)	167, 179, 614, 884, 1359, 1607, 1723, 1814, 1923
AsuII	TTCGAA	1 cut(s)	1669
AsuNHI	GCTAGC	1 cut(s)	28
AvaII	GGWCC	4 cut(s)	921, 1361, 1795, 2927
AvrII	CCTAGG	1 cut(s)	317
AxyI	CCTNAGG	2 cut(s)	192, 1896
BaeGI	GKGCMC	2 cut(s)	967, 1885
BalI	TGGCCA	2 cut(s)	722, 890
BanI	GGYRCC	2 cut(s)	962, 2737
BanII	GRGCYC	2 cut(s)	1225, 1299
BarI	GAAGNNNNNNTAC	2 cut(s)	275, 307
BauI	CACGAG	1 cut(s)	2378
BbsI	GAAGAC	5 cut(s)	629, 1422, 1584, 2027, 2566
Bbv12I	GWGCWC	2 cut(s)	615, 1225
BbvCI	CCTCAGC	1 cut(s)	3048
BbvI	GCAGC	9 cut(s)	346, 383, 387, 395, 787, 805, 1441, 1861, 2632
BccI	CCATC	8 cut(s)	18, 262, 299, 552, 1599, 2635, 2658, 3115
BceAI	ACGGC	5 cut(s)	148, 883, 1360, 2272, 2635
BcgI	CGANNNNNNTGC	2 cut(s)	2372, 2406
BciT130I	CCWGG	9 cut(s)	483, 555, 654, 960, 1602, 2544, 2913, 2925, 2982
BciVI	GTATCC	2 cut(s)	51, 2227
BclI	TGATCA	1 cut(s)	2716
BcnI	CCSGG	2 cut(s)	346, 600
BcoDI	GTCTC	2 cut(s)	2884, 3091
BfaI	CTAG	5 cut(s)	29, 318, 1808, 2529, 2745
BfmI	CTRYAG	1 cut(s)	19
BfuI	GTATCC	2 cut(s)	51, 2227
BglI	GCCNNNNNGGC	1 cut(s)	320
BlnI	CCTAGG	1 cut(s)	317
BmcAI	AGTACT	1 cut(s)	524
Bme18I	GGWCC	4 cut(s)	921, 1361, 1795, 2927
BmgBI	CACGTC	1 cut(s)	674
BmgT120I	GGNCC	6 cut(s)	921, 1361, 1795, 2854, 2927, 2975
BmiI	GGNNCC	5 cut(s)	923, 964, 2548, 2739, 3009
BmtI	GCTAGC	1 cut(s)	32
BpiI	GAAGAC	5 cut(s)	629, 1422, 1584, 2027, 2566
BplI	GAGNNNNNCTC	2 cut(s)	3088, 3120
BpmI	CTGGAG	4 cut(s)	873, 1079, 2298, 2565
Bpu10I	CCTNAGC	1 cut(s)	3048
Bpu14I	TTCGAA	1 cut(s)	1669
BpuEI	CTTGAG	3 cut(s)	1508, 1775, 2748
BpuMI	CCSGG	2 cut(s)	346, 600
BsaI	GGTCTC	2 cut(s)	2884, 3091
BsaJI	CCNNGG	8 cut(s)	278, 317, 717, 879, 1798, 2489, 2684, 2912
BsaWI	WCCGGW	2 cut(s)	76, 103
BsaXI	ACNNNNNCTCC	2 cut(s)	2890, 2920
Bsc4I	CCNNNNNNNGG	9 cut(s)	605, 1897, 2229, 2276, 2402, 2686, 2734, 3025, 3071
Bse118I	RCCGGY	1 cut(s)	76
Bse1I	ACTGG	5 cut(s)	114, 822, 904, 1062, 2936
Bse21I	CCTNAGG	2 cut(s)	192, 1896
Bse3DI	GCAATG	3 cut(s)	515, 676, 2629
BseBI	CCWGG	9 cut(s)	483, 555, 654, 960, 1602, 2544, 2913, 2925, 2982
BseDI	CCNNGG	8 cut(s)	278, 317, 717, 879, 1798, 2489, 2684, 2912
BseGI	GGATG	9 cut(s)	563, 700, 1003, 1610, 2131, 2256, 2668, 2758, 3107
BseLI	CCNNNNNNNGG	9 cut(s)	605, 1897, 2229, 2276, 2402, 2686, 2734, 3025, 3071
BseMI	GCAATG	3 cut(s)	515, 676, 2629
BseMII	CTCAG	6 cut(s)	183, 1461, 1887, 2034, 2861, 3062
BseNI	ACTGG	5 cut(s)	114, 822, 904, 1062, 2936
BseRI	GAGGAG	5 cut(s)	422, 1296, 1398, 2090, 2524
BseSI	GKGCMC	2 cut(s)	967, 1885
BseXI	GCAGC	9 cut(s)	346, 383, 387, 395, 787, 805, 1441, 1861, 2632
BseYI	CCCAGC	2 cut(s)	770, 3111
BsgI	GTGCAG	1 cut(s)	2436
Bsh1285I	CGRYCG	1 cut(s)	108
BshFI	GGCC	8 cut(s)	202, 277, 722, 890, 2515, 2855, 2977, 3120
BshNI	GGYRCC	2 cut(s)	962, 2737
BshTI	ACCGGT	1 cut(s)	76
BsiEI	CGRYCG	1 cut(s)	108
BsiHKAI	GWGCWC	2 cut(s)	615, 1225
BsiSI	CCGG	4 cut(s)	77, 104, 346, 599
BslFI	GGGAC	1 cut(s)	2736
BslI	CCNNNNNNNGG	9 cut(s)	605, 1897, 2229, 2276, 2402, 2686, 2734, 3025, 3071
BsmAI	GTCTC	2 cut(s)	2884, 3091
BsmFI	GGGAC	1 cut(s)	2736
BsmI	GAATGC	1 cut(s)	448
BsnI	GGCC	8 cut(s)	202, 277, 722, 890, 2515, 2855, 2977, 3120
Bso31I	GGTCTC	2 cut(s)	2884, 3091
Bsp119I	TTCGAA	1 cut(s)	1669
Bsp1286I	GDGCHC	5 cut(s)	615, 967, 1225, 1299, 1885
Bsp1407I	TGTACA	2 cut(s)	291, 2374
Bsp19I	CCATGG	1 cut(s)	2489
BspANI	GGCC	8 cut(s)	202, 277, 722, 890, 2515, 2855, 2977, 3120
BspCNI	CTCAG	6 cut(s)	184, 1462, 1888, 2035, 2860, 3061
BspHI	TCATGA	1 cut(s)	1492
BspLI	GGNNCC	5 cut(s)	923, 964, 2548, 2739, 3009
BspOI	GCTAGC	1 cut(s)	32
BspPI	GGATC	6 cut(s)	336, 545, 656, 1399, 1916, 2213
BspT104I	TTCGAA	1 cut(s)	1669
BspT107I	GGYRCC	2 cut(s)	962, 2737
BspTNI	GGTCTC	2 cut(s)	2884, 3091
BsrBI	CCGCTC	1 cut(s)	2579
BsrDI	GCAATG	3 cut(s)	515, 676, 2629
BsrFI	RCCGGY	1 cut(s)	76
BsrGI	TGTACA	2 cut(s)	291, 2374
BsrI	ACTGG	5 cut(s)	114, 822, 904, 1062, 2936
BssAI	RCCGGY	1 cut(s)	76
BssECI	CCNNGG	8 cut(s)	278, 317, 717, 879, 1798, 2489, 2684, 2912
BssSI	CACGAG	1 cut(s)	2378
BssT1I	CCWWGG	3 cut(s)	317, 717, 2489
Bst2BI	CACGAG	1 cut(s)	2378
Bst2UI	CCWGG	9 cut(s)	483, 555, 654, 960, 1602, 2544, 2913, 2925, 2982
Bst4CI	ACNGT	5 cut(s)	291, 1447, 1845, 2096, 2732
Bst6I	CTCTTC	3 cut(s)	1273, 1620, 1666
BstAPI	GCANNNNNTGC	1 cut(s)	377
BstAUI	TGTACA	2 cut(s)	291, 2374
BstBI	TTCGAA	1 cut(s)	1669
BstC8I	GCNNGC	7 cut(s)	30, 714, 1144, 1148, 1242, 1423, 2451
BstDEI	CTNAG	9 cut(s)	192, 536, 926, 1470, 1896, 2043, 2418, 2847, 3048
BstDSI	CCRYGG	2 cut(s)	2489, 2684
BstENI	CCTNNNNNAGG	1 cut(s)	1895
BstF5I	GGATG	9 cut(s)	563, 700, 1003, 1610, 2131, 2256, 2668, 2758, 3107
BstHHI	GCGC	2 cut(s)	990, 1774
BstMAI	GTCTC	2 cut(s)	2884, 3091
BstMCI	CGRYCG	1 cut(s)	108
BstNI	CCWGG	9 cut(s)	483, 555, 654, 960, 1602, 2544, 2913, 2925, 2982
BstNSI	RCATGY	3 cut(s)	241, 2241, 2609
BstSFI	CTRYAG	1 cut(s)	19
BstSLI	GKGCMC	2 cut(s)	967, 1885
BstV1I	GCAGC	9 cut(s)	346, 383, 387, 395, 787, 805, 1441, 1861, 2632
BstV2I	GAAGAC	5 cut(s)	629, 1422, 1584, 2027, 2566
BstX2I	RGATCY	2 cut(s)	341, 1391
BstYI	RGATCY	2 cut(s)	341, 1391
Bsu36I	CCTNAGG	2 cut(s)	192, 1896
BsuI	GTATCC	2 cut(s)	51, 2227
BsuRI	GGCC	8 cut(s)	202, 277, 722, 890, 2515, 2855, 2977, 3120
BtgI	CCRYGG	2 cut(s)	2489, 2684
BtgZI	GCGATG	1 cut(s)	2586
BtrI	CACGTC	1 cut(s)	674
BtsCI	GGATG	9 cut(s)	563, 700, 1003, 1610, 2131, 2256, 2668, 2758, 3107
BtsI	GCAGTG	2 cut(s)	1754, 2248
BtsIMutI	CAGTG	6 cut(s)	239, 1055, 1692, 1754, 2101, 2248
Cac8I	GCNNGC	7 cut(s)	30, 714, 1144, 1148, 1242, 1423, 2451
CciI	TCATGA	1 cut(s)	1492
CfoI	GCGC	2 cut(s)	990, 1774
Cfr10I	RCCGGY	1 cut(s)	76
Cfr13I	GGNCC	6 cut(s)	921, 1361, 1795, 2854, 2927, 2975
CseI	GACGC	2 cut(s)	1247, 1298
Csp6I	GTAC	7 cut(s)	151, 292, 523, 901, 2375, 2842, 3067
CspAI	ACCGGT	1 cut(s)	76
CviQI	GTAC	7 cut(s)	151, 292, 523, 901, 2375, 2842, 3067
DdeI	CTNAG	9 cut(s)	192, 536, 926, 1470, 1896, 2043, 2418, 2847, 3048
DraI	TTTAAA	1 cut(s)	1840
DrdI	GACNNNNNNGTC	1 cut(s)	2342
DseDI	GACNNNNNNGTC	1 cut(s)	2342
EaeI	YGGCCR	3 cut(s)	720, 888, 3118
Eam1104I	CTCTTC	3 cut(s)	1273, 1620, 1666
EarI	CTCTTC	3 cut(s)	1273, 1620, 1666
EciI	GGCGGA	1 cut(s)	2280
Ecl136II	GAGCTC	1 cut(s)	1223
Eco130I	CCWWGG	3 cut(s)	317, 717, 2489
Eco147I	AGGCCT	1 cut(s)	202
Eco24I	GRGCYC	2 cut(s)	1225, 1299
Eco31I	GGTCTC	2 cut(s)	2884, 3091
Eco32I	GATATC	1 cut(s)	1130
Eco47I	GGWCC	4 cut(s)	921, 1361, 1795, 2927
Eco53kI	GAGCTC	1 cut(s)	1223
Eco57I	CTGAAG	6 cut(s)	408, 1256, 1341, 1416, 1593, 1716
Eco81I	CCTNAGG	2 cut(s)	192, 1896
EcoICRI	GAGCTC	1 cut(s)	1223
EcoNI	CCTNNNNNAGG	1 cut(s)	1895
EcoO109I	RGGNCCY	3 cut(s)	1361, 1795, 2927
EcoRII	CCWGG	9 cut(s)	481, 553, 652, 958, 1600, 2542, 2911, 2923, 2980
EcoRV	GATATC	1 cut(s)	1130
EcoT14I	CCWWGG	3 cut(s)	317, 717, 2489
EcoT22I	ATGCAT	1 cut(s)	1441
EcoT38I	GRGCYC	2 cut(s)	1225, 1299
ErhI	CCWWGG	3 cut(s)	317, 717, 2489
FalI	AAGNNNNNCTT	2 cut(s)	2019, 2051
FaqI	GGGAC	1 cut(s)	2736
FauI	CCCGC	1 cut(s)	1155
FauNDI	CATATG	4 cut(s)	1047, 2348, 2518, 2659
FbaI	TGATCA	1 cut(s)	2716
FblI	GTMKAC	1 cut(s)	108
FokI	GGATG	9 cut(s)	570, 707, 1010, 1617, 2118, 2263, 2655, 2745, 3094
FriOI	GRGCYC	2 cut(s)	1225, 1299
FspBI	CTAG	5 cut(s)	29, 318, 1808, 2529, 2745
GlaI	GCGC	2 cut(s)	989, 1773
GsaI	CCCAGC	2 cut(s)	774, 3115
GsuI	CTGGAG	4 cut(s)	873, 1079, 2298, 2565
HaeIII	GGCC	8 cut(s)	202, 277, 722, 890, 2515, 2855, 2977, 3120
HapII	CCGG	4 cut(s)	77, 104, 346, 599
HgaI	GACGC	2 cut(s)	1247, 1298
HhaI	GCGC	2 cut(s)	990, 1774
Hin6I	GCGC	2 cut(s)	988, 1772
HinP1I	GCGC	2 cut(s)	988, 1772
HincII	GTYRAC	1 cut(s)	109
HindII	GTYRAC	1 cut(s)	109
HindIII	AAGCTT	1 cut(s)	431
HinfI	GANTC	7 cut(s)	331, 464, 955, 1063, 1313, 2081, 2474
HpaII	CCGG	4 cut(s)	77, 104, 346, 599
HphI	GGTGA	9 cut(s)	167, 179, 614, 884, 1359, 1607, 1723, 1814, 1923
Hpy166II	GTNNAC	6 cut(s)	109, 1598, 1805, 2174, 2377, 2603
Hpy8I	GTNNAC	6 cut(s)	109, 1598, 1805, 2174, 2377, 2603
Hpy99I	CGWCG	5 cut(s)	53, 56, 59, 92, 770
HpyAV	CCTTC	9 cut(s)	213, 599, 824, 841, 1374, 1691, 1724, 1901, 2240
HpyCH4III	ACNGT	5 cut(s)	291, 1447, 1845, 2096, 2732
HpyCH4IV	ACGT	3 cut(s)	673, 1657, 2194
HpyF3I	CTNAG	9 cut(s)	192, 536, 926, 1470, 1896, 2043, 2418, 2847, 3048
HpySE526I	ACGT	3 cut(s)	673, 1657, 2194
HspAI	GCGC	2 cut(s)	988, 1772
Ksp22I	TGATCA	1 cut(s)	2716
LmnI	GCTCC	4 cut(s)	610, 1168, 2546, 2911
Lsp1109I	GCAGC	9 cut(s)	346, 383, 387, 395, 787, 805, 1441, 1861, 2632
MaeI	CTAG	5 cut(s)	29, 318, 1808, 2529, 2745
MaeII	ACGT	3 cut(s)	673, 1657, 2194
MaeIII	GTNAC	4 cut(s)	1688, 2119, 2834, 2960
MbiI	CCGCTC	1 cut(s)	2579
MfeI	CAATTG	3 cut(s)	300, 510, 2177
MflI	RGATCY	2 cut(s)	341, 1391
MhlI	GDGCHC	5 cut(s)	615, 967, 1225, 1299, 1885
MlsI	TGGCCA	2 cut(s)	722, 890
MluNI	TGGCCA	2 cut(s)	722, 890
MmeI	TCCRAC	2 cut(s)	1006, 1168
Mox20I	TGGCCA	2 cut(s)	722, 890
Mph1103I	ATGCAT	1 cut(s)	1441
MroXI	GAANNNNTTC	2 cut(s)	1488, 1665
MscI	TGGCCA	2 cut(s)	722, 890
MslI	CAYNNNNRTG	2 cut(s)	1747, 2865
Msp20I	TGGCCA	2 cut(s)	722, 890
MspA1I	CMGCKG	2 cut(s)	386, 1855
MspI	CCGG	4 cut(s)	77, 104, 346, 599
MunI	CAATTG	3 cut(s)	300, 510, 2177
Mva1269I	GAATGC	1 cut(s)	448
MvaI	CCWGG	9 cut(s)	483, 555, 654, 960, 1602, 2544, 2913, 2925, 2982
NciI	CCSGG	2 cut(s)	346, 600
NcoI	CCATGG	1 cut(s)	2489
NdeI	CATATG	4 cut(s)	1047, 2348, 2518, 2659
NheI	GCTAGC	1 cut(s)	28
NlaIV	GGNNCC	5 cut(s)	923, 964, 2548, 2739, 3009
NmeAIII	GCCGAG	1 cut(s)	904
NmuCI	GTSAC	1 cut(s)	2834
NsiI	ATGCAT	1 cut(s)	1441
NspI	RCATGY	3 cut(s)	241, 2241, 2609
NspV	TTCGAA	1 cut(s)	1669
PagI	TCATGA	1 cut(s)	1492
PceI	AGGCCT	1 cut(s)	202
PciI	ACATGT	1 cut(s)	2605
PcsI	WCGNNNNNNNCGW	1 cut(s)	54
PctI	GAATGC	1 cut(s)	448
PdmI	GAANNNNTTC	2 cut(s)	1488, 1665
PfeI	GAWTC	7 cut(s)	331, 464, 955, 1063, 1313, 2081, 2474
PflMI	CCANNNNNTGG	3 cut(s)	2276, 2734, 3025
PfoI	TCCNGGA	3 cut(s)	481, 553, 2542
PinAI	ACCGGT	1 cut(s)	76
PpuMI	RGGWCCY	3 cut(s)	1361, 1795, 2927
PscI	ACATGT	1 cut(s)	2605
Psp124BI	GAGCTC	1 cut(s)	1225
Psp5II	RGGWCCY	3 cut(s)	1361, 1795, 2927
Psp6I	CCWGG	9 cut(s)	481, 553, 652, 958, 1600, 2542, 2911, 2923, 2980
PspFI	CCCAGC	2 cut(s)	770, 3111
PspGI	CCWGG	9 cut(s)	481, 553, 652, 958, 1600, 2542, 2911, 2923, 2980
PspN4I	GGNNCC	5 cut(s)	923, 964, 2548, 2739, 3009
PspPI	GGNCC	6 cut(s)	921, 1361, 1795, 2854, 2927, 2975
PspPPI	RGGWCCY	3 cut(s)	1361, 1795, 2927
PsuI	RGATCY	2 cut(s)	341, 1391
PvuII	CAGCTG	2 cut(s)	386, 1855
RsaI	GTAC	7 cut(s)	152, 293, 524, 902, 2376, 2843, 3068
RsaNI	GTAC	7 cut(s)	151, 292, 523, 901, 2375, 2842, 3067
RseI	CAYNNNNRTG	2 cut(s)	1747, 2865
SacI	GAGCTC	1 cut(s)	1225
SalI	GTCGAC	1 cut(s)	107
Sau96I	GGNCC	6 cut(s)	921, 1361, 1795, 2854, 2927, 2975
ScaI	AGTACT	1 cut(s)	524
SduI	GDGCHC	5 cut(s)	615, 967, 1225, 1299, 1885
SfcI	CTRYAG	1 cut(s)	19
SfuI	TTCGAA	1 cut(s)	1669
SinI	GGWCC	4 cut(s)	921, 1361, 1795, 2927
SmiMI	CAYNNNNRTG	2 cut(s)	1747, 2865
SmlI	CTYRAG	3 cut(s)	1523, 1790, 2763
SmoI	CTYRAG	3 cut(s)	1523, 1790, 2763
SseBI	AGGCCT	1 cut(s)	202
SspI	AATATT	3 cut(s)	916, 935, 1209
SspMI	CTAG	5 cut(s)	29, 318, 1808, 2529, 2745
SstI	GAGCTC	1 cut(s)	1225
StuI	AGGCCT	1 cut(s)	202
StyI	CCWWGG	3 cut(s)	317, 717, 2489
TaaI	ACNGT	5 cut(s)	291, 1447, 1845, 2096, 2732
TaiI	ACGT	3 cut(s)	676, 1660, 2197
TatI	WGTACW	3 cut(s)	291, 522, 2374
TauI	GCSGC	5 cut(s)	277, 383, 2579, 2980, 3123
TfiI	GAWTC	7 cut(s)	331, 464, 955, 1063, 1313, 2081, 2474
TscAI	CASTG	6 cut(s)	239, 1062, 1699, 1754, 2101, 2248
TseFI	GTSAC	1 cut(s)	2834
TseI	GCWGC	9 cut(s)	359, 371, 383, 400, 775, 818, 1454, 1874, 2645
Tsp45I	GTSAC	1 cut(s)	2834
TspGWI	ACGGA	3 cut(s)	859, 2021, 2087
TspRI	CASTG	6 cut(s)	239, 1062, 1699, 1754, 2101, 2248
Van91I	CCANNNNNTGG	3 cut(s)	2276, 2734, 3025
VpaK11BI	GGWCC	4 cut(s)	921, 1361, 1795, 2927
XagI	CCTNNNNNAGG	1 cut(s)	1895
XapI	RAATTY	4 cut(s)	1109, 1153, 2779, 2827
XbaI	TCTAGA	1 cut(s)	2528
XceI	RCATGY	3 cut(s)	241, 2241, 2609
XmaJI	CCTAGG	1 cut(s)	317
XmiI	GTMKAC	1 cut(s)	108
XmnI	GAANNNNTTC	2 cut(s)	1488, 1665
XspI	CTAG	5 cut(s)	29, 318, 1808, 2529, 2745
ZrmI	AGTACT	1 cut(s)	524
Zsp2I	ATGCAT	1 cut(s)	1441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.