MD09G1038100.v1.1

Gibberellin-regulated protein 14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001842
Physical Location & Seq
Forward (+)
2324761 .. 2326415
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1038100.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGCTTCCAAAGGCATGCTCCTTCTCTTTGCAACCATTCTCCTTTTCACTGCGAGGGCTTCATCACTTGATCATCATGACGAACTCAAGATTAAGATTAATTATGTCAAGGCAACAGCTCCACCACCTCTATCCAAGGCTCCAGCGCCGCCGCCTCTTGTGGCCAAACCACCCACTCCACCAATTGCCAAGCCGCCAACTCCACCACTTACCAAGCCATCACCACCAATTCACAAGCTCCCTACTCCTCCTTGCCCCCACCACCCCCACAGCCACCAAGCCACCAACTCCACCACTTACCAAGCCATCACCACCAATTCACAAGCTCCCTACTCCTCCCCTTGCCAAGCCACCAACTCCACCACTTACCAAGCCACCAACTCCACCACTTACCAAGCCATCACCACCAATTTACAAGCCACCTGCTCCTCCACTTACCAAGCCACCATCTCCTCCGCTTGCCAAGCCACCATCTCCTCCGTATACCAAGCCAACTCCTGTCATCCCACCAGTGAAGCCACCAACTCCGGTCATCCCCCCAGTGAATCCACCAAGTCCTGTCAGTCCCCCAGTGAAGCCACCCACTTATCCACCGCTTCCCCCGATTGCATCCCACTGTGTGACAAGAGGTGCATGCTGCATTCAAGGAAGAGGGTGTGCATGAGAGCTTGCACGACGTGCTGCGACCGCTGCCGCTGCGTTCCTCCGGGAACATTCGGCAACCGGGAAAGATGCGGCAAGTGCTACACCGATATGGTCACCCATGGCAACAGAAGCAAGTGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GASA

Protein Analysis

263

Amino Acids

28.06

Weight (kDa)

9.07

Isoelectric Point (pI)

52.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GASA PF02704 202 - 262 1.3e-19 Gibberellin regulated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 431
AccI GTMKAC 1 cut(s) 483
AciI CCGC 8 cut(s) 149, 152, 194, 456, 594, 688, 694, 735
AcoI YGGCCR 1 cut(s) 162
AdeI CACNNNGTG 2 cut(s) 620, 678
AgsI TTSAA 1 cut(s) 645
AjiI CACGTC 1 cut(s) 678
AluBI AGCT 4 cut(s) 119, 238, 326, 668
AluI AGCT 4 cut(s) 119, 238, 326, 668
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 4 cut(s) 637, 681, 690, 696
AseI ATTAAT 1 cut(s) 99
AspLEI GCGC 1 cut(s) 148
AsuC2I CCSGG 2 cut(s) 708, 725
AsuHPI GGTGA 4 cut(s) 213, 301, 394, 751
BaeGI GKGCMC 1 cut(s) 785
BalI TGGCCA 1 cut(s) 164
BbvI GCAGC 4 cut(s) 624, 668, 677, 683
BccI CCATC 5 cut(s) 226, 314, 407, 455, 479
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 2 cut(s) 708, 725
BfoI RGCGCY 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 431
BisI GCNGC 9 cut(s) 149, 152, 194, 638, 682, 691, 694, 697, 736
BlsI GCNGC 9 cut(s) 150, 153, 195, 639, 683, 692, 695, 698, 737
Bme1390I CCNGG 2 cut(s) 708, 725
BmgBI CACGTC 1 cut(s) 678
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 2 cut(s) 708, 725
BmrI ACTGGG 2 cut(s) 534, 564
BmsI GCATC 2 cut(s) 618, 722
BmuI ACTGGG 2 cut(s) 534, 564
BpmI CTGGAG 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 708, 725
BsaJI CCNNGG 2 cut(s) 135, 763
BsaWI WCCGGW 1 cut(s) 527
Bse1I ACTGG 3 cut(s) 510, 540, 570
BseDI CCNNGG 2 cut(s) 135, 763
BseGI GGATG 3 cut(s) 502, 532, 609
BseNI ACTGG 3 cut(s) 510, 540, 570
BseRI GAGGAG 5 cut(s) 237, 325, 418, 442, 466
BseSI GKGCMC 1 cut(s) 785
BseXI GCAGC 4 cut(s) 624, 668, 677, 683
Bsh1285I CGRYCG 1 cut(s) 688
BshFI GGCC 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 688
BsiSI CCGG 3 cut(s) 528, 707, 724
BslFI GGGAC 1 cut(s) 550
BsmFI GGGAC 1 cut(s) 550
BsmI GAATGC 1 cut(s) 640
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 785
Bsp143I GATC 1 cut(s) 70
Bsp19I CCATGG 1 cut(s) 763
BspACI CCGC 8 cut(s) 149, 152, 194, 456, 594, 688, 694, 735
BspANI GGCC 1 cut(s) 164
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 431
BsrI ACTGG 3 cut(s) 510, 540, 570
BssECI CCNNGG 2 cut(s) 135, 763
BssMI GATC 1 cut(s) 70
BssNAI GTATAC 1 cut(s) 484
BssT1I CCWWGG 2 cut(s) 135, 763
Bst1107I GTATAC 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 618
Bst6I CTCTTC 1 cut(s) 644
BstAPI GCANNNNNTGC 1 cut(s) 678
BstC8I GCNNGC 4 cut(s) 17, 460, 635, 670
BstDSI CCRYGG 1 cut(s) 763
BstEII GGTNACC 1 cut(s) 757
BstF5I GGATG 3 cut(s) 502, 532, 609
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 1 cut(s) 73
BstMBI GATC 1 cut(s) 70
BstMCI CGRYCG 1 cut(s) 688
BstMWI GCNNNNNNNGC 7 cut(s) 464, 678, 687, 690, 696, 741, 774
BstNSI RCATGY 2 cut(s) 19, 637
BstPI GGTNACC 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 706, 723
BstSLI GKGCMC 1 cut(s) 785
BstV1I GCAGC 4 cut(s) 624, 668, 677, 683
BstZ17I GTATAC 1 cut(s) 484
BsuRI GGCC 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 763
BtrI CACGTC 1 cut(s) 678
BtsCI GGATG 3 cut(s) 502, 532, 609
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 5 cut(s) 48, 517, 547, 577, 614
BveI ACCTGC 1 cut(s) 431
Cac8I GCNNGC 4 cut(s) 17, 460, 635, 670
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 148
CviAII CATG 5 cut(s) 16, 77, 634, 661, 764
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
DraIII CACNNNGTG 2 cut(s) 620, 678
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 135, 763
Eco91I GGTNACC 1 cut(s) 757
EcoO65I GGTNACC 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 135, 763
ErhI CCWWGG 2 cut(s) 135, 763
FaeI CATG 5 cut(s) 19, 80, 637, 664, 767
FaiI YATR 8 cut(s) 17, 78, 105, 484, 635, 662, 755, 765
FaqI GGGAC 1 cut(s) 550
FatI CATG 5 cut(s) 15, 76, 633, 660, 763
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 9 cut(s) 149, 152, 194, 638, 682, 691, 694, 697, 736
FokI GGATG 3 cut(s) 489, 519, 596
Fsp4HI GCNGC 9 cut(s) 149, 152, 194, 638, 682, 691, 694, 697, 736
GlaI GCGC 1 cut(s) 147
GluI GCNGC 9 cut(s) 149, 152, 194, 638, 682, 691, 694, 697, 736
GsuI CTGGAG 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 164
HapII CCGG 3 cut(s) 528, 707, 724
HhaI GCGC 1 cut(s) 148
Hin1II CATG 5 cut(s) 19, 80, 637, 664, 767
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 1 cut(s) 545
HpaII CCGG 3 cut(s) 528, 707, 724
HphI GGTGA 4 cut(s) 213, 301, 394, 751
Hpy166II GTNNAC 1 cut(s) 484
Hpy188III TCNNGA 2 cut(s) 77, 88
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 1 cut(s) 679
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 1 cut(s) 618
HpyCH4IV ACGT 1 cut(s) 677
HpyCH4V TGCA 6 cut(s) 32, 609, 633, 640, 660, 672
HpyF10VI GCNNNNNNNGC 7 cut(s) 464, 678, 687, 690, 696, 741, 774
HpySE526I ACGT 1 cut(s) 677
Hsp92II CATG 5 cut(s) 19, 80, 637, 664, 767
HspAI GCGC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 6 cut(s) 24, 124, 145, 243, 331, 431
Lsp1109I GCAGC 4 cut(s) 624, 668, 677, 683
LweI GCATC 2 cut(s) 618, 722
MaeII ACGT 1 cut(s) 677
MaeIII GTNAC 2 cut(s) 620, 757
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 1 cut(s) 661
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 785
MlsI TGGCCA 1 cut(s) 164
MluCI AATT 5 cut(s) 100, 183, 228, 316, 409
MluNI TGGCCA 1 cut(s) 164
Mox20I TGGCCA 1 cut(s) 164
MscI TGGCCA 1 cut(s) 164
MseI TTAA 2 cut(s) 93, 99
MslI CAYNNNNRTG 1 cut(s) 752
Msp20I TGGCCA 1 cut(s) 164
MspA1I CMGCKG 2 cut(s) 690, 696
MspI CCGG 3 cut(s) 528, 707, 724
MspR9I CCNGG 2 cut(s) 708, 725
MunI CAATTG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 640
MwoI GCNNNNNNNGC 7 cut(s) 464, 678, 687, 690, 696, 741, 774
NciI CCSGG 2 cut(s) 708, 725
NcoI CCATGG 1 cut(s) 763
NdeII GATC 1 cut(s) 70
NlaIII CATG 5 cut(s) 19, 80, 637, 664, 767
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 620, 757
NspI RCATGY 2 cut(s) 19, 637
PaeI GCATGC 2 cut(s) 19, 637
PagI TCATGA 1 cut(s) 76
PaqCI CACCTGC 1 cut(s) 431
PctI GAATGC 1 cut(s) 640
PfeI GAWTC 1 cut(s) 545
PfoI TCCNGGA 1 cut(s) 706
PkrI GCNGC 9 cut(s) 150, 153, 195, 639, 683, 692, 695, 698, 737
PshBI ATTAAT 1 cut(s) 99
PspEI GGTNACC 1 cut(s) 757
PspN4I GGNNCC 1 cut(s) 141
RseI CAYNNNNRTG 1 cut(s) 752
SaqAI TTAA 2 cut(s) 93, 99
SatI GCNGC 9 cut(s) 149, 152, 194, 638, 682, 691, 694, 697, 736
Sau3AI GATC 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 708, 725
SduI GDGCHC 1 cut(s) 785
SetI ASST 8 cut(s) 121, 130, 240, 328, 425, 632, 670, 680
SfaNI GCATC 2 cut(s) 618, 722
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SphI GCATGC 2 cut(s) 19, 637
Sse9I AATT 5 cut(s) 100, 183, 228, 316, 409
SsiI CCGC 8 cut(s) 149, 152, 194, 456, 594, 688, 694, 735
StyD4I CCNGG 2 cut(s) 706, 723
StyI CCWWGG 2 cut(s) 135, 763
TaaI ACNGT 1 cut(s) 618
TaiI ACGT 1 cut(s) 680
TasI AATT 5 cut(s) 100, 183, 228, 316, 409
TauI GCSGC 5 cut(s) 151, 154, 196, 696, 738
TfiI GAWTC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 93, 99
Tru9I TTAA 2 cut(s) 93, 99
TscAI CASTG 5 cut(s) 55, 517, 547, 577, 621
TseFI GTSAC 2 cut(s) 620, 757
TseI GCWGC 4 cut(s) 637, 681, 690, 696
Tsp45I GTSAC 2 cut(s) 620, 757
TspDTI ATGAA 1 cut(s) 51
TspGWI ACGGA 1 cut(s) 469
TspRI CASTG 5 cut(s) 55, 517, 547, 577, 621
VspI ATTAAT 1 cut(s) 99
XceI RCATGY 2 cut(s) 19, 637
XmiI GTMKAC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.