MD17G1039400.v1.1

Gibberellin-regulated protein 14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001842
Physical Location & Seq
Forward (+)
2888529 .. 2889943
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1039400.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGCTTCCAAAACCGTGCTCCTTCTCTTCGCAACTGTTCTCCTTTTCACTGCTAGGGCTTCATCACTTGATGATCATGAGGAACTCGCGATTAAGTCTACTTATGGCAATGCACCATCGCCACAACCTCTGGTCAAGGCACCCAGTCCACCCCTTGCCAAGCCACCGTGTCCACCACTAGCCAAGCCACCATCTCCACTATATACCAAGCCTCCATCTCCTGTCAGCCCCCCAGTGAAGCCACCAACTCCTGACAGTCCCCCAGTGATGCCACCCACTTATCCACCACTTCCCCCGGTCAGGTCCAAATCAGATTGCGTCCCATTGTGTGAGAAGAGGTGCATGCAGCATTCAAGGAAGAGGCCGTGCATGAGAGCTTGCACGGCGTGCTGCGACCGCTGCCGCTGCGTTCCTCCGGGAACATTCAGCAACGAGGAAAAATGCGGCCAGTGCTACACCGATATTGTCATCCATGGCATCAGAACCAAATGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GASA

Protein Analysis

166

Amino Acids

17.84

Weight (kDa)

9.1

Isoelectric Point (pI)

82.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GASA PF02704 105 - 165 6.1e-18 Gibberellin regulated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 98
AccII CGCG 1 cut(s) 89
AciI CCGC 3 cut(s) 397, 403, 444
AcoI YGGCCR 1 cut(s) 445
AdeI CACNNNGTG 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 300
AgsI TTSAA 1 cut(s) 354
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw21I GWGCWC 1 cut(s) 21
AoxI GGCC 2 cut(s) 362, 445
ApeKI GCWGC 4 cut(s) 346, 390, 399, 405
AspS9I GGNCC 1 cut(s) 303
AsuC2I CCSGG 2 cut(s) 296, 417
AvaII GGWCC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 21
BbvI GCAGC 4 cut(s) 358, 377, 386, 392
BccI CCATC 3 cut(s) 124, 199, 223
BceAI ACGGC 2 cut(s) 349, 399
BclI TGATCA 1 cut(s) 73
BcnI CCSGG 2 cut(s) 296, 417
BfaI CTAG 2 cut(s) 54, 179
BisI GCNGC 6 cut(s) 347, 391, 400, 403, 406, 445
BlsI GCNGC 6 cut(s) 348, 392, 401, 404, 407, 446
Bme1390I CCNGG 2 cut(s) 296, 417
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 2 cut(s) 296, 417
BmrI ACTGGG 3 cut(s) 138, 227, 257
BmsI GCATC 2 cut(s) 258, 486
BmuI ACTGGG 3 cut(s) 138, 227, 257
BpuMI CCSGG 2 cut(s) 296, 417
BsaJI CCNNGG 2 cut(s) 294, 472
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 300
Bse1I ACTGG 4 cut(s) 144, 233, 263, 448
Bse3DI GCAATG 1 cut(s) 115
BseDI CCNNGG 2 cut(s) 294, 472
BseGI GGATG 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 300
BseMI GCAATG 1 cut(s) 115
BseNI ACTGG 4 cut(s) 144, 233, 263, 448
BseXI GCAGC 4 cut(s) 358, 377, 386, 392
Bsh1236I CGCG 1 cut(s) 89
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 364, 447
BshNI GGYRCC 1 cut(s) 139
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 21
BsiSI CCGG 2 cut(s) 296, 416
BslFI GGGAC 2 cut(s) 243, 305
BslI CCNNNNNNNGG 1 cut(s) 300
BsmFI GGGAC 2 cut(s) 243, 305
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 2 cut(s) 364, 447
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 472
Bsp68I TCGCGA 1 cut(s) 89
BspACI CCGC 3 cut(s) 397, 403, 444
BspANI GGCC 2 cut(s) 364, 447
BspFNI CGCG 1 cut(s) 89
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 115
BsrI ACTGG 4 cut(s) 144, 233, 263, 448
BssECI CCNNGG 2 cut(s) 294, 472
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 472
Bst4CI ACNGT 4 cut(s) 16, 37, 168, 257
Bst6I CTCTTC 3 cut(s) 32, 329, 353
BstAPI GCANNNNNTGC 1 cut(s) 387
BstC8I GCNNGC 3 cut(s) 344, 379, 388
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 1 cut(s) 468
BstFNI CGCG 1 cut(s) 89
BstKTI GATC 1 cut(s) 76
BstMBI GATC 1 cut(s) 73
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 6 cut(s) 383, 387, 396, 399, 405, 450
BstNSI RCATGY 1 cut(s) 346
BstSCI CCNGG 2 cut(s) 294, 415
BstUI CGCG 1 cut(s) 89
BstV1I GCAGC 4 cut(s) 358, 377, 386, 392
BsuRI GGCC 2 cut(s) 364, 447
BtgI CCRYGG 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 1 cut(s) 468
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 4 cut(s) 48, 240, 270, 455
BtuMI TCGCGA 1 cut(s) 89
Cac8I GCNNGC 3 cut(s) 344, 379, 388
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 303
CseI GACGC 1 cut(s) 307
CviAII CATG 4 cut(s) 77, 343, 370, 473
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 387
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 3 cut(s) 32, 329, 353
EarI CTCTTC 3 cut(s) 32, 329, 353
Eco130I CCWWGG 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 472
FaeI CATG 4 cut(s) 80, 346, 373, 476
FaiI YATR 7 cut(s) 78, 105, 202, 204, 344, 371, 474
FaqI GGGAC 2 cut(s) 243, 305
FatI CATG 4 cut(s) 76, 342, 369, 472
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 6 cut(s) 347, 391, 400, 403, 406, 445
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 6 cut(s) 347, 391, 400, 403, 406, 445
FspBI CTAG 2 cut(s) 54, 179
GluI GCNGC 6 cut(s) 347, 391, 400, 403, 406, 445
HaeIII GGCC 2 cut(s) 364, 447
HapII CCGG 2 cut(s) 296, 416
HgaI GACGC 1 cut(s) 307
Hin1II CATG 4 cut(s) 80, 346, 373, 476
HpaII CCGG 2 cut(s) 296, 416
Hpy166II GTNNAC 3 cut(s) 99, 149, 173
Hpy188I TCNGA 2 cut(s) 313, 482
Hpy188III TCNNGA 3 cut(s) 77, 88, 251
Hpy8I GTNNAC 3 cut(s) 99, 149, 173
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 4 cut(s) 16, 37, 168, 257
HpyCH4V TGCA 5 cut(s) 113, 342, 346, 369, 381
HpyF10VI GCNNNNNNNGC 6 cut(s) 383, 387, 396, 399, 405, 450
Hsp92II CATG 4 cut(s) 80, 346, 373, 476
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 1 cut(s) 24
Lsp1109I GCAGC 4 cut(s) 358, 377, 386, 392
LweI GCATC 2 cut(s) 258, 486
MaeI CTAG 2 cut(s) 54, 179
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 3 cut(s) 19, 346, 370
MhlI GDGCHC 1 cut(s) 21
MnlI CCTC 7 cut(s) 73, 138, 222, 330, 354, 423, 427
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 2 cut(s) 399, 405
MspI CCGG 2 cut(s) 296, 416
MspR9I CCNGG 2 cut(s) 296, 417
Mva1269I GAATGC 1 cut(s) 349
MvnI CGCG 1 cut(s) 89
MwoI GCNNNNNNNGC 6 cut(s) 383, 387, 396, 399, 405, 450
NciI CCSGG 2 cut(s) 296, 417
NcoI CCATGG 1 cut(s) 472
NdeII GATC 1 cut(s) 73
NlaIII CATG 4 cut(s) 80, 346, 373, 476
NlaIV GGNNCC 1 cut(s) 141
NruI TCGCGA 1 cut(s) 89
NspI RCATGY 1 cut(s) 346
PaeI GCATGC 1 cut(s) 346
PagI TCATGA 1 cut(s) 76
PctI GAATGC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 6 cut(s) 348, 392, 401, 404, 407, 446
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 303
RruI TCGCGA 1 cut(s) 89
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 6 cut(s) 347, 391, 400, 403, 406, 445
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 1 cut(s) 303
ScrFI CCNGG 2 cut(s) 296, 417
SduI GDGCHC 1 cut(s) 21
SetI ASST 4 cut(s) 130, 305, 341, 379
SfaNI GCATC 2 cut(s) 258, 486
SinI GGWCC 1 cut(s) 303
SphI GCATGC 1 cut(s) 346
SsiI CCGC 3 cut(s) 397, 403, 444
SspMI CTAG 2 cut(s) 54, 179
StyD4I CCNGG 2 cut(s) 294, 415
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 4 cut(s) 16, 37, 168, 257
TauI GCSGC 2 cut(s) 405, 447
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 4 cut(s) 55, 240, 270, 455
TseI GCWGC 4 cut(s) 346, 390, 399, 405
TspDTI ATGAA 1 cut(s) 51
TspRI CASTG 4 cut(s) 55, 240, 270, 455
VpaK11BI GGWCC 1 cut(s) 303
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 98
XspI CTAG 2 cut(s) 54, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.