MD17G1039200.v1.1

Gibberellin-regulated protein 14-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001842
Physical Location & Seq
Forward (+)
2862655 .. 2864327
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1039200.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGCTTCCAAAACCATGCTCCTTCTCTTTGCAACTGTTCTCCTTTTCACTGCTAGGGCTTCATCACTTGACGATCATGAGGAACTCAAGATTAAGGTTACTTATGTCAAGGCACCAGCTCCACAACCTCTAGTCAAGGCTCCAGCTCCGCCGCCTCTTGTAGTCAAGGCACCCAGTCCACCACTTTCCAAGCCACCGTCTCCACCACTTGCCAAGCCACCATCTCCACCATATACCAAGCCACCAACTCCTGTCAGGCCCCCAGTGAAGCCACCAACTCCTGTCAGTCCCCCAGTGATGCCACCCACTTATCCACCACTTCCTCCGGTTAGGTCCAAAGCAGATTGCATCCCATTGTGTGACAAGAGGTGCATGTTGCATTCAAGGAAGAGGGTGTGCATGAGAGCTTGCATGACGTGCTGCGACCGCTGCCGCTGCGTTCCTCCGGGAACATTCGGCAACCAGGAAAGATGCGGCAAGTGCTACACCGATATGGTCACCCATGGCAACAGAACCAAATGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GASA

Protein Analysis

176

Amino Acids

19.04

Weight (kDa)

9.67

Isoelectric Point (pI)

74.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GASA PF02704 115 - 175 6.2e-20 Gibberellin regulated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 112, 169
AciI CCGC 5 cut(s) 149, 152, 427, 433, 474
AgsI TTSAA 1 cut(s) 384
AjiI CACGTC 1 cut(s) 417
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 3 cut(s) 119, 146, 407
AluI AGCT 3 cut(s) 119, 146, 407
Alw26I GTCTC 1 cut(s) 204
AoxI GGCC 1 cut(s) 257
ApeKI GCWGC 3 cut(s) 420, 429, 435
AspS9I GGNCC 2 cut(s) 258, 333
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 490
AvaII GGWCC 1 cut(s) 333
BanI GGYRCC 2 cut(s) 112, 169
BbvI GCAGC 3 cut(s) 407, 416, 422
BccI CCATC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 464
BcnI CCSGG 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 2 cut(s) 54, 131
BisI GCNGC 6 cut(s) 152, 421, 430, 433, 436, 475
BlsI GCNGC 6 cut(s) 153, 422, 431, 434, 437, 476
Bme1390I CCNGG 2 cut(s) 447, 464
Bme18I GGWCC 1 cut(s) 333
BmgBI CACGTC 1 cut(s) 417
BmgT120I GGNCC 2 cut(s) 258, 333
BmiI GGNNCC 4 cut(s) 114, 141, 171, 260
BmrFI CCNGG 2 cut(s) 447, 464
BmrI ACTGGG 3 cut(s) 168, 257, 287
BmsI GCATC 3 cut(s) 288, 357, 461
BmuI ACTGGG 3 cut(s) 168, 257, 287
BpmI CTGGAG 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 502
BsaWI WCCGGW 1 cut(s) 325
Bse1I ACTGG 3 cut(s) 174, 263, 293
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 502
BseGI GGATG 1 cut(s) 348
BseNI ACTGG 3 cut(s) 174, 263, 293
BseXI GCAGC 3 cut(s) 407, 416, 422
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 1 cut(s) 259
BshNI GGYRCC 2 cut(s) 112, 169
BsiEI CGRYCG 1 cut(s) 427
BsiSI CCGG 2 cut(s) 326, 446
BslFI GGGAC 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 204
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 273
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 1 cut(s) 259
Bsp143I GATC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 5 cut(s) 149, 152, 427, 433, 474
BspANI GGCC 1 cut(s) 259
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 114, 141, 171, 260
BspT107I GGYRCC 2 cut(s) 112, 169
BsrI ACTGG 3 cut(s) 174, 263, 293
BssECI CCNNGG 1 cut(s) 502
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 502
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 37, 198
Bst6I CTCTTC 1 cut(s) 383
BstAPI GCANNNNNTGC 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 409
BstDSI CCRYGG 1 cut(s) 502
BstEII GGTNACC 1 cut(s) 496
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 1 cut(s) 73
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 5 cut(s) 417, 426, 429, 435, 480
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 1 cut(s) 376
BstPI GGTNACC 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 445, 462
BstV1I GCAGC 3 cut(s) 407, 416, 422
BsuRI GGCC 1 cut(s) 259
BtgI CCRYGG 1 cut(s) 502
BtrI CACGTC 1 cut(s) 417
BtsCI GGATG 1 cut(s) 348
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 3 cut(s) 48, 270, 300
Cac8I GCNNGC 1 cut(s) 409
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 258, 333
CviAII CATG 6 cut(s) 16, 77, 373, 400, 412, 503
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
EciI GGCGGA 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 333
Eco91I GGTNACC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 6 cut(s) 19, 80, 376, 403, 415, 506
FaqI GGGAC 1 cut(s) 273
FatI CATG 6 cut(s) 15, 76, 372, 399, 411, 502
Fnu4HI GCNGC 6 cut(s) 152, 421, 430, 433, 436, 475
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 6 cut(s) 152, 421, 430, 433, 436, 475
FspBI CTAG 2 cut(s) 54, 131
GluI GCNGC 6 cut(s) 152, 421, 430, 433, 436, 475
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 1 cut(s) 259
HapII CCGG 2 cut(s) 326, 446
Hin1II CATG 6 cut(s) 19, 80, 376, 403, 415, 506
HpaII CCGG 2 cut(s) 326, 446
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 1 cut(s) 179
Hpy188III TCNNGA 2 cut(s) 77, 88
Hpy8I GTNNAC 1 cut(s) 179
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 2 cut(s) 37, 198
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 6 cut(s) 32, 348, 372, 379, 399, 411
HpyF10VI GCNNNNNNNGC 5 cut(s) 417, 426, 429, 435, 480
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 6 cut(s) 19, 80, 376, 403, 415, 506
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 4 cut(s) 24, 124, 145, 151
Lsp1109I GCAGC 3 cut(s) 407, 416, 422
LweI GCATC 3 cut(s) 288, 357, 461
MaeI CTAG 2 cut(s) 54, 131
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 3 cut(s) 97, 359, 496
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 1 cut(s) 400
MnlI CCTC 7 cut(s) 73, 138, 165, 333, 360, 384, 453
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 2 cut(s) 429, 435
MspI CCGG 2 cut(s) 326, 446
MspR9I CCNGG 2 cut(s) 447, 464
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 5 cut(s) 417, 426, 429, 435, 480
NciI CCSGG 1 cut(s) 447
NcoI CCATGG 1 cut(s) 502
NdeII GATC 1 cut(s) 73
NlaIII CATG 6 cut(s) 19, 80, 376, 403, 415, 506
NlaIV GGNNCC 4 cut(s) 114, 141, 171, 260
NmuCI GTSAC 2 cut(s) 359, 496
NspI RCATGY 1 cut(s) 376
PagI TCATGA 1 cut(s) 76
PctI GAATGC 1 cut(s) 379
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 6 cut(s) 153, 422, 431, 434, 437, 476
Psp6I CCWGG 1 cut(s) 462
PspEI GGTNACC 1 cut(s) 496
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 4 cut(s) 114, 141, 171, 260
PspPI GGNCC 2 cut(s) 258, 333
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 6 cut(s) 152, 421, 430, 433, 436, 475
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 2 cut(s) 258, 333
ScrFI CCNGG 2 cut(s) 447, 464
SetI ASST 8 cut(s) 99, 121, 130, 148, 335, 371, 409, 419
SfaNI GCATC 3 cut(s) 288, 357, 461
SinI GGWCC 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SsiI CCGC 5 cut(s) 149, 152, 427, 433, 474
SspMI CTAG 2 cut(s) 54, 131
StyD4I CCNGG 2 cut(s) 445, 462
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 2 cut(s) 37, 198
TaiI ACGT 1 cut(s) 419
TauI GCSGC 3 cut(s) 154, 435, 477
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 3 cut(s) 55, 270, 300
TseFI GTSAC 2 cut(s) 359, 496
TseI GCWGC 3 cut(s) 420, 429, 435
Tsp45I GTSAC 2 cut(s) 359, 496
TspDTI ATGAA 1 cut(s) 51
TspRI CASTG 3 cut(s) 55, 270, 300
VpaK11BI GGWCC 1 cut(s) 333
XceI RCATGY 1 cut(s) 376
XspI CTAG 2 cut(s) 54, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.