MD10G1203100.v1.1

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003409
Physical Location & Seq
Reverse (-)
30087736 .. 30089827
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1203100.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGAGCGCAAAGACAGTCAGTCTCCTCAAGGTGAGATAGCTCGGATTCTGGTAGAATTCCTGGAGGTTGCAATCACTTCAGTCGTTTTCCTCAAAGGAATTTACCCTCCAGGTACTTTTGAAAGAAGGAAATATATGAATTTGGTGGTTCATAGAGCTCGTCACCTTGAACTCAGAGATTACATCCATTCCGCTGTCTCGGGACTTCTTCCTTTTATCCAAAAGGGTCTCGTGGAGAGAGTAGCTGTTATTTTCTTTAACGGTGACAACATCCCTGTGGAAAGATTTATGTTTAAGCTCAATGTGAATCAATCGTATGGCTCGAGGGTGGAAGAATCTGACCTGGAGTTCTCACTAAGGTCATTTTTTATCAAGCTTCCTATCTCAGAACCCCTTACCAAGGCTCTTCCCCAGAATTGCAGGTGGGAGATAACTGCTTACTTTCGATCACTCCCTCAGGCTTGTACAAGTAAGGATGCAGAGTCGTGGATTCCAACAGATACAAAGCAGTGGCAACAACCTCCGTTAATAACCCCTATTAAGTCAATGAGTAGCCAACCTCTATCTGTGCAGTTATATTTGGAACATCCAAGTTTATCTGAACCAAGGGCTTGA

Protein Analysis

205

Amino Acids

23.48

Weight (kDa)

8.53

Isoelectric Point (pI)

62.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HORMA PF02301 18 - 157 9.3e-08 HORMA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 411
Acc36I ACCTGC 1 cut(s) 411
AciI CCGC 1 cut(s) 192
AcsI RAATTY 3 cut(s) 56, 99, 139
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 2 cut(s) 115, 466
AfiI CCNNNNNNNGG 1 cut(s) 400
AgsI TTSAA 2 cut(s) 122, 170
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 3 cut(s) 60, 109, 342
AluBI AGCT 5 cut(s) 41, 158, 245, 298, 376
AluI AGCT 5 cut(s) 41, 158, 245, 298, 376
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 3 cut(s) 27, 202, 233
Ama87I CYCGRG 2 cut(s) 199, 322
ApoI RAATTY 3 cut(s) 56, 99, 139
AspLEI GCGC 1 cut(s) 9
AsuHPI GGTGA 3 cut(s) 44, 155, 275
AvaI CYCGRG 2 cut(s) 199, 322
AxyI CCTNAGG 1 cut(s) 456
BanII GRGCYC 1 cut(s) 160
BauI CACGAG 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 62, 111, 344
BcoDI GTCTC 3 cut(s) 27, 202, 233
BfuAI ACCTGC 1 cut(s) 411
Bme1390I CCNGG 3 cut(s) 62, 111, 344
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 2 cut(s) 199, 322
BmrFI CCNGG 3 cut(s) 62, 111, 344
BmsI GCATC 1 cut(s) 466
BpmI CTGGAG 3 cut(s) 83, 93, 365
BpuEI CTTGAG 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 399, 605
Bsc4I CCNNNNNNNGG 1 cut(s) 400
Bse21I CCTNAGG 1 cut(s) 456
BseBI CCWGG 3 cut(s) 62, 111, 344
BseDI CCNNGG 2 cut(s) 399, 605
BseGI GGATG 4 cut(s) 183, 270, 481, 586
BseLI CCNNNNNNNGG 1 cut(s) 400
BseMII CTCAG 3 cut(s) 187, 399, 470
BseRI GAGGAG 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 590
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 2 cut(s) 199, 322
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 400
BsmAI GTCTC 3 cut(s) 27, 202, 233
BsmFI GGGAC 1 cut(s) 216
Bso31I GGTCTC 1 cut(s) 233
BsoBI CYCGRG 2 cut(s) 199, 322
Bsp1286I GDGCHC 1 cut(s) 160
Bsp1407I TGTACA 1 cut(s) 464
Bsp143I GATC 1 cut(s) 446
BspACI CCGC 1 cut(s) 192
BspCNI CTCAG 3 cut(s) 186, 398, 469
BspMI ACCTGC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 411
BspTNI GGTCTC 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 464
BssECI CCNNGG 2 cut(s) 399, 605
BssMI GATC 1 cut(s) 446
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 2 cut(s) 399, 605
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 3 cut(s) 62, 111, 344
Bst4CI ACNGT 2 cut(s) 17, 263
Bst6I CTCTTC 1 cut(s) 411
BstAUI TGTACA 1 cut(s) 464
BstDEI CTNAG 4 cut(s) 173, 356, 385, 456
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 4 cut(s) 183, 270, 481, 586
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 449
BstMAI GTCTC 3 cut(s) 27, 202, 233
BstMBI GATC 1 cut(s) 446
BstNI CCWGG 3 cut(s) 62, 111, 344
BstSCI CCNGG 3 cut(s) 60, 109, 342
Bsu36I CCTNAGG 1 cut(s) 456
BtsCI GGATG 4 cut(s) 183, 270, 481, 586
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
BveI ACCTGC 1 cut(s) 411
CfoI GCGC 1 cut(s) 9
Csp6I GTAC 2 cut(s) 114, 465
CviQI GTAC 2 cut(s) 114, 465
DdeI CTNAG 4 cut(s) 173, 356, 385, 456
DpnI GATC 1 cut(s) 448
DpnII GATC 1 cut(s) 446
DriI GACNNNNNGTC 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 411
Eam1105I GACNNNNNGTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 399, 605
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 63
Eco81I CCTNAGG 1 cut(s) 456
Eco88I CYCGRG 2 cut(s) 199, 322
EcoICRI GAGCTC 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 3 cut(s) 60, 109, 342
EcoT14I CCWWGG 2 cut(s) 399, 605
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 399, 605
FaiI YATR 6 cut(s) 135, 137, 153, 290, 318, 577
FaqI GGGAC 1 cut(s) 216
FokI GGATG 4 cut(s) 170, 257, 488, 573
FriOI GRGCYC 1 cut(s) 160
GlaI GCGC 1 cut(s) 8
GsuI CTGGAG 3 cut(s) 83, 93, 365
HhaI GCGC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 5 cut(s) 46, 307, 335, 482, 490
HphI GGTGA 3 cut(s) 44, 155, 275
Hpy188I TCNGA 5 cut(s) 45, 176, 340, 388, 601
Hpy188III TCNNGA 1 cut(s) 201
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 2 cut(s) 17, 263
HpyCH4V TGCA 4 cut(s) 71, 420, 479, 571
HpyF3I CTNAG 4 cut(s) 173, 356, 385, 456
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 446
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 4
LweI GCATC 1 cut(s) 466
MaeIII GTNAC 2 cut(s) 161, 263
MalI GATC 1 cut(s) 448
MboI GATC 1 cut(s) 446
MboII GAAGA 3 cut(s) 200, 344, 398
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 4 cut(s) 56, 99, 139, 415
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 8 cut(s) 36, 58, 101, 117, 318, 465, 531, 570
MseI TTAA 4 cut(s) 258, 294, 527, 540
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 62, 111, 344
MvaI CCWGG 3 cut(s) 62, 111, 344
NdeII GATC 1 cut(s) 446
NmuCI GTSAC 2 cut(s) 161, 263
PaeR7I CTCGAG 1 cut(s) 322
PaqCI CACCTGC 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PfeI GAWTC 4 cut(s) 46, 307, 335, 490
PfoI TCCNGGA 1 cut(s) 60
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 3 cut(s) 60, 109, 342
PspGI CCWGG 3 cut(s) 60, 109, 342
PspXI VCTCGAGB 1 cut(s) 322
RsaI GTAC 2 cut(s) 115, 466
RsaNI GTAC 2 cut(s) 114, 465
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 160
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 4 cut(s) 258, 294, 527, 540
Sau3AI GATC 1 cut(s) 446
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 3 cut(s) 62, 111, 344
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 1 cut(s) 466
Sfr274I CTCGAG 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 27, 322
SmoI CTYRAG 2 cut(s) 27, 322
Sse9I AATT 4 cut(s) 56, 99, 139, 415
SsiI CCGC 1 cut(s) 192
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 3 cut(s) 60, 109, 342
StyI CCWWGG 2 cut(s) 399, 605
TaaI ACNGT 2 cut(s) 17, 263
TaqI TCGA 2 cut(s) 323, 445
TasI AATT 4 cut(s) 56, 99, 139, 415
TatI WGTACW 1 cut(s) 464
TfiI GAWTC 4 cut(s) 46, 307, 335, 490
Tru1I TTAA 4 cut(s) 258, 294, 527, 540
Tru9I TTAA 4 cut(s) 258, 294, 527, 540
TscAI CASTG 1 cut(s) 515
TseFI GTSAC 2 cut(s) 161, 263
Tsp45I GTSAC 2 cut(s) 161, 263
TspDTI ATGAA 2 cut(s) 140, 152
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 1 cut(s) 515
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 3 cut(s) 56, 99, 139
XhoI CTCGAG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.