RchiOBHm_Chr5g0026331

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003409
Physical Location & Seq
Forward (+)
20334819 .. 20338017
3199 bp
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UTR
Exon/CDS
Intron
PRQ30591

Sequence Viewer

Length: 615 bp
ATGGAGCGCAAAGACAATCAGTCTCCTCAAGATGAAACCGCTCGGATTCTGGTCGAGTTCTTGGAGGTGGCAATCACTTCAATCGTTTTCCTCAAAGGAATTTACCCCCCAGGTGCTTTTGAGAGAAGGAAGTATATGAATTTGGTGGTTCATAGAGCTCGGCACCCTGAGCTCAGAGATTATATCCATTCTGCTGTCTCAGGACTTCTTCCTTTTATCAAAAAGGGTCTGGTGGAGAGAGTAGCAGTTATTTTCTTCGACAGTGACAACAACCCTATGGAAAGATTTATGTTTAAGCTCACTGTGGATCAGTCTTGTGGCTCAAACGTCAAAGAAGCTGACCTGGAGTTCTCACTTAGGTCATTCTTTATCAAGCTTCCTCTCATAGAATCCGCTACTAAGGTTCTTCCTCAGAATTGCAGGTGGGAGATAACGGCATACTTCCAGTCCCTTCCTCAGGAAATTTCAAGCAAGAATGAAGGGTTGTGGCTTCCAACAGACACAAAGCAGTGGCAACAACCTCCTCTAATAACTCCTATTAAGTCGATGAGTAGCCAACCTCTCTCTGTACAGTTGTATCTAGAACATCCAAGTGTATATGAACCAAAGCCATGA

Protein Analysis

204

Amino Acids

23.56

Weight (kDa)

6.91

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HORMA PF02301 18 - 111 5.5e-09 HORMA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 411
Acc36I ACCTGC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 41
AciI CCGC 2 cut(s) 39, 393
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 3 cut(s) 99, 139, 462
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 2 cut(s) 81, 468
AhdI GACNNNNNGTC 1 cut(s) 19
AjnI CCWGG 2 cut(s) 109, 342
AluBI AGCT 5 cut(s) 158, 172, 298, 338, 376
AluI AGCT 5 cut(s) 158, 172, 298, 338, 376
Alw21I GWGCWC 2 cut(s) 160, 174
Alw26I GTCTC 2 cut(s) 27, 202
AlwI GGATC 1 cut(s) 315
ApoI RAATTY 3 cut(s) 99, 139, 462
AspLEI GCGC 1 cut(s) 9
AxyI CCTNAGG 1 cut(s) 456
BaeI ACNNNNGTAYC 2 cut(s) 560, 593
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 2 cut(s) 160, 174
Bbv12I GWGCWC 2 cut(s) 160, 174
BceAI ACGGC 1 cut(s) 450
BciT130I CCWGG 2 cut(s) 111, 344
BcoDI GTCTC 2 cut(s) 27, 202
BfaI CTAG 1 cut(s) 581
BfuAI ACCTGC 1 cut(s) 411
Bme1390I CCNGG 2 cut(s) 111, 344
BmeRI GACNNNNNGTC 1 cut(s) 19
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 111, 344
BpmI CTGGAG 1 cut(s) 365
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 445
Bse21I CCTNAGG 1 cut(s) 456
BseBI CCWGG 2 cut(s) 111, 344
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMII CTCAG 5 cut(s) 159, 187, 213, 425, 470
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 2 cut(s) 15, 513
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 2 cut(s) 160, 174
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 2 cut(s) 27, 202
BsmFI GGGAC 1 cut(s) 433
Bsp1286I GDGCHC 2 cut(s) 160, 174
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 2 cut(s) 39, 393
BspCNI CTCAG 5 cut(s) 160, 186, 212, 424, 469
BspLI GGNNCC 1 cut(s) 164
BspMI ACCTGC 1 cut(s) 411
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 568
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 1 cut(s) 307
Bst2UI CCWGG 2 cut(s) 111, 344
Bst4CI ACNGT 3 cut(s) 263, 304, 573
BstAUI TGTACA 1 cut(s) 568
BstDEI CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 1 cut(s) 586
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 2 cut(s) 27, 202
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 2 cut(s) 111, 344
BstSCI CCNGG 2 cut(s) 109, 342
Bsu36I CCTNAGG 1 cut(s) 456
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 3 cut(s) 268, 300, 515
BveI ACCTGC 1 cut(s) 411
CfoI GCGC 1 cut(s) 9
Csp6I GTAC 1 cut(s) 569
CviAII CATG 1 cut(s) 612
CviJI RGCY 9 cut(s) 158, 172, 298, 321, 338, 376, 490, 555, 610
CviKI_1 RGCY 9 cut(s) 158, 172, 298, 321, 338, 376, 490, 555, 610
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
DriI GACNNNNNGTC 1 cut(s) 19
Eam1105I GACNNNNNGTC 1 cut(s) 19
Ecl136II GAGCTC 2 cut(s) 158, 172
Eco24I GRGCYC 2 cut(s) 160, 174
Eco53kI GAGCTC 2 cut(s) 158, 172
Eco81I CCTNAGG 1 cut(s) 456
EcoICRI GAGCTC 2 cut(s) 158, 172
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 109, 342
EcoT38I GRGCYC 2 cut(s) 160, 174
FaeI CATG 1 cut(s) 615
FaqI GGGAC 1 cut(s) 433
FatI CATG 1 cut(s) 611
FokI GGATG 1 cut(s) 573
FriOI GRGCYC 2 cut(s) 160, 174
FspBI CTAG 1 cut(s) 581
GlaI GCGC 1 cut(s) 8
GsuI CTGGAG 1 cut(s) 365
HhaI GCGC 1 cut(s) 9
Hin1II CATG 1 cut(s) 615
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 46, 389
Hpy188I TCNGA 3 cut(s) 45, 176, 414
Hpy188III TCNNGA 4 cut(s) 29, 201, 458, 581
HpyAV CCTTC 3 cut(s) 120, 461, 473
HpyCH4III ACNGT 3 cut(s) 263, 304, 573
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 7 cut(s) 168, 173, 199, 356, 399, 411, 456
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 1 cut(s) 615
HspAI GCGC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 4
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 263
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 307
MboII GAAGA 3 cut(s) 200, 247, 398
MhlI GDGCHC 2 cut(s) 160, 174
MluCI AATT 4 cut(s) 99, 139, 415, 462
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 9 cut(s) 36, 58, 101, 390, 420, 465, 531, 534, 570
MseI TTAA 2 cut(s) 294, 540
MslI CAYNNNNRTG 1 cut(s) 591
MspR9I CCNGG 2 cut(s) 111, 344
MvaI CCWGG 2 cut(s) 111, 344
MwoI GCNNNNNNNGC 1 cut(s) 169
NdeII GATC 1 cut(s) 307
NlaIII CATG 1 cut(s) 615
NlaIV GGNNCC 1 cut(s) 164
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 263
PaqCI CACCTGC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 46, 389
Psp124BI GAGCTC 2 cut(s) 160, 174
Psp6I CCWGG 2 cut(s) 109, 342
PspGI CCWGG 2 cut(s) 109, 342
PspN4I GGNNCC 1 cut(s) 164
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 591
SacI GAGCTC 2 cut(s) 160, 174
SaqAI TTAA 2 cut(s) 294, 540
Sau3AI GATC 1 cut(s) 307
ScrFI CCNGG 2 cut(s) 111, 344
SduI GDGCHC 2 cut(s) 160, 174
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 4 cut(s) 99, 139, 415, 462
SsiI CCGC 2 cut(s) 39, 393
SspMI CTAG 1 cut(s) 581
SstI GAGCTC 2 cut(s) 160, 174
StyD4I CCNGG 2 cut(s) 109, 342
TaaI ACNGT 3 cut(s) 263, 304, 573
TaiI ACGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 54, 258, 545
TasI AATT 4 cut(s) 99, 139, 415, 462
TatI WGTACW 1 cut(s) 568
TfiI GAWTC 2 cut(s) 46, 389
Tru1I TTAA 2 cut(s) 294, 540
Tru9I TTAA 2 cut(s) 294, 540
TscAI CASTG 3 cut(s) 268, 307, 515
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 5 cut(s) 48, 140, 152, 492, 615
TspRI CASTG 3 cut(s) 268, 307, 515
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 3 cut(s) 99, 139, 462
XbaI TCTAGA 1 cut(s) 580
XspI CTAG 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.