Rh5DG184000

Mitotic spindle assembly checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003409
Physical Location & Seq
Forward (+)
20380954 .. 20385307
4354 bp
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UTR
Exon/CDS
Intron
Rh5DG184000.1

Sequence Viewer

Length: 678 bp
ATGGCCATATCCAGATTCATATATTTTGTTAACCATCACAAATTGTCTTTTGATTGTTTTCGTGTTTTTGGTATGCTTCAGCAACTAAAGCTTGTCCTGGGTTCAATTATTTGGGCATTTCGGCACACAGAAAGAAATATTGCTGAAACTGGACTGAACCCGTTATTGGAGATGCTGAAGAACTTTCAGGTCAATCAATTTTTGAAAGGACTATTCAAGTCCAAGAATGACTGCTTGGCCAGATTTAAGGGAGACTTTCTGTTGCAATTAAAAGAATTTTCAGCACAGGGTCTGGTGGAGAGAGTAGCAGTTATTTTCTTCGACAGTGACAACAACCCTATGGAAAGATTTATGTTTAAGCTCACTGTGGATCAGTCTTGTGGCTCAAACGTCAAAGAAGCTGACCTGGAGTTCTCACTTAGGTCATTCTTTATCAAGCTTCCTCTCATAGAATCCGCTACTAAGGTTCTTCCTCAGAATTGCAGGTGGGAGATAACGGCATACTTCCAGTCCCTTCCTCAGGAAATTTCAAGCAAGAATGAAGGGTTGTGGCTTCCAACAGACACAAAGCAGTGGCAACAACCTCCTCTAATAACTCCTATTAAGTCGATGAGTAGCCAACCTCTCTCTGTACAGTTGTATCTAGAACATCCAAGTGTATATGAACCAAAGCCATGA

Protein Analysis

225

Amino Acids

26.12

Weight (kDa)

8.63

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRM1_C PF08767 16 - 64 3.2e-10 CRM1 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 474
Acc36I ACCTGC 1 cut(s) 474
AciI CCGC 1 cut(s) 456
AclWI GGATC 1 cut(s) 378
AcoI YGGCCR 2 cut(s) 3, 237
AcsI RAATTY 2 cut(s) 275, 525
AcuI CTGAAG 2 cut(s) 62, 197
AfaI GTAC 1 cut(s) 633
AfiI CCNNNNNNNGG 2 cut(s) 166, 520
AgsI TTSAA 4 cut(s) 105, 205, 217, 531
AjnI CCWGG 2 cut(s) 96, 405
AjuI GAANNNNNNNTTGG 4 cut(s) 149, 181, 218, 250
AluBI AGCT 4 cut(s) 91, 361, 401, 439
AluI AGCT 4 cut(s) 91, 361, 401, 439
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 378
AlwNI CAGNNNCTG 1 cut(s) 292
AoxI GGCC 2 cut(s) 3, 237
ApoI RAATTY 2 cut(s) 275, 525
AxyI CCTNAGG 1 cut(s) 519
BaeI ACNNNNGTAYC 2 cut(s) 623, 656
BalI TGGCCA 2 cut(s) 5, 239
BccI CCATC 1 cut(s) 42
BceAI ACGGC 1 cut(s) 513
BciT130I CCWGG 2 cut(s) 98, 407
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 644
BfuAI ACCTGC 1 cut(s) 474
Bme1390I CCNGG 2 cut(s) 98, 407
BmrFI CCNGG 2 cut(s) 98, 407
BmsI GCATC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 97
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 520
Bse1I ACTGG 2 cut(s) 154, 508
Bse21I CCTNAGG 1 cut(s) 519
BseBI CCWGG 2 cut(s) 98, 407
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 2 cut(s) 166, 520
BseMII CTCAG 2 cut(s) 488, 533
BseNI ACTGG 2 cut(s) 154, 508
BseRI GAGGAG 1 cut(s) 576
BshFI GGCC 2 cut(s) 5, 239
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 2 cut(s) 166, 520
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 496
BsnI GGCC 2 cut(s) 5, 239
Bsp1407I TGTACA 1 cut(s) 631
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 5, 239
BspCNI CTCAG 2 cut(s) 487, 532
BspMI ACCTGC 1 cut(s) 474
BspPI GGATC 1 cut(s) 378
BsrGI TGTACA 1 cut(s) 631
BsrI ACTGG 2 cut(s) 154, 508
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 1 cut(s) 370
Bst2UI CCWGG 2 cut(s) 98, 407
Bst4CI ACNGT 3 cut(s) 326, 367, 636
BstAUI TGTACA 1 cut(s) 631
BstDEI CTNAG 4 cut(s) 419, 462, 474, 519
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 1 cut(s) 649
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 2 cut(s) 98, 407
BstSCI CCNGG 2 cut(s) 96, 405
Bsu36I CCTNAGG 1 cut(s) 519
BsuRI GGCC 2 cut(s) 5, 239
BtsCI GGATG 1 cut(s) 649
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 3 cut(s) 331, 363, 578
BveI ACCTGC 1 cut(s) 474
CaiI CAGNNNCTG 1 cut(s) 292
Csp6I GTAC 1 cut(s) 632
CviAII CATG 1 cut(s) 675
CviQI GTAC 1 cut(s) 632
DdeI CTNAG 4 cut(s) 419, 462, 474, 519
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
EaeI YGGCCR 2 cut(s) 3, 237
Eco57I CTGAAG 2 cut(s) 62, 197
Eco81I CCTNAGG 1 cut(s) 519
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoRII CCWGG 2 cut(s) 96, 405
FaeI CATG 1 cut(s) 678
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 496
FatI CATG 1 cut(s) 674
FokI GGATG 1 cut(s) 636
FspBI CTAG 1 cut(s) 644
GsuI CTGGAG 1 cut(s) 428
HaeIII GGCC 2 cut(s) 5, 239
Hin1II CATG 1 cut(s) 678
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 2 cut(s) 89, 437
HinfI GANTC 2 cut(s) 15, 452
HpaI GTTAAC 1 cut(s) 31
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 477
Hpy188III TCNNGA 3 cut(s) 12, 521, 644
Hpy8I GTNNAC 1 cut(s) 31
HpyAV CCTTC 2 cut(s) 524, 536
HpyCH4III ACNGT 3 cut(s) 326, 367, 636
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 2 cut(s) 265, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 4 cut(s) 419, 462, 474, 519
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 1 cut(s) 678
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 370
LweI GCATC 1 cut(s) 162
MaeI CTAG 1 cut(s) 644
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 1 cut(s) 326
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 3 cut(s) 190, 310, 461
MlsI TGGCCA 2 cut(s) 5, 239
MluCI AATT 7 cut(s) 41, 105, 197, 266, 275, 478, 525
MluNI TGGCCA 2 cut(s) 5, 239
MmeI TCCRAC 1 cut(s) 581
MnlI CCTC 6 cut(s) 453, 483, 528, 594, 597, 633
Mox20I TGGCCA 2 cut(s) 5, 239
MscI TGGCCA 2 cut(s) 5, 239
MseI TTAA 5 cut(s) 30, 246, 269, 357, 603
MslI CAYNNNNRTG 1 cut(s) 654
Msp20I TGGCCA 2 cut(s) 5, 239
MspR9I CCNGG 2 cut(s) 98, 407
MvaI CCWGG 2 cut(s) 98, 407
MwoI GCNNNNNNNGC 1 cut(s) 88
NdeII GATC 1 cut(s) 370
NlaIII CATG 1 cut(s) 678
NmuCI GTSAC 1 cut(s) 326
PaqCI CACCTGC 1 cut(s) 474
PfeI GAWTC 2 cut(s) 15, 452
Psp6I CCWGG 2 cut(s) 96, 405
PspGI CCWGG 2 cut(s) 96, 405
PstNI CAGNNNCTG 1 cut(s) 292
RsaI GTAC 1 cut(s) 633
RsaNI GTAC 1 cut(s) 632
RseI CAYNNNNRTG 1 cut(s) 654
SaqAI TTAA 5 cut(s) 30, 246, 269, 357, 603
Sau3AI GATC 1 cut(s) 370
ScrFI CCNGG 2 cut(s) 98, 407
SfaNI GCATC 1 cut(s) 162
SmiMI CAYNNNNRTG 1 cut(s) 654
Sse9I AATT 7 cut(s) 41, 105, 197, 266, 275, 478, 525
SsiI CCGC 1 cut(s) 456
SspI AATATT 1 cut(s) 139
SspMI CTAG 1 cut(s) 644
StyD4I CCNGG 2 cut(s) 96, 405
TaaI ACNGT 3 cut(s) 326, 367, 636
TaiI ACGT 1 cut(s) 393
TaqI TCGA 2 cut(s) 321, 608
TasI AATT 7 cut(s) 41, 105, 197, 266, 275, 478, 525
TatI WGTACW 1 cut(s) 631
TfiI GAWTC 2 cut(s) 15, 452
Tru1I TTAA 5 cut(s) 30, 246, 269, 357, 603
Tru9I TTAA 5 cut(s) 30, 246, 269, 357, 603
TscAI CASTG 3 cut(s) 331, 370, 578
TseFI GTSAC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 3 cut(s) 7, 555, 678
TspRI CASTG 3 cut(s) 331, 370, 578
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 2 cut(s) 275, 525
XbaI TCTAGA 1 cut(s) 643
XspI CTAG 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.